SitesBLAST

451 Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cations; C→M: Transport activity strongly reduced.

O15244 Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2 from Homo sapiens (Human) (see 8 papers)
29% identity, 22% coverage: 71:179/489 of query aligns to 154:251/555 of O15244

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O15244

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M165 (≠ I85) to I: lower Vmax for MPP(+) transport; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177507
Y169 (≠ H89) mutation to F: No change in TEA uptake.
T201 (≠ E121) to M: in dbSNP:rs145450955
Y241 (≠ T169) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377.

Sites not aligning to the query:

54 P → S: in dbSNP:rs8177504
73 Y→F: No change in TEA uptake.
92 Y→F: No change in TEA uptake.
128 Y→F: No change in TEA uptake.
257 Y→F: No change in TEA uptake.
270 S → A: decreased Ki value for TBA inhibition of MPP(+); no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs316019
279 Y→F: No change in TEA uptake.
280 Y→F: No change in TEA uptake.
284 P→A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
284:288 Proline-rich sequence
286 S→A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation.
287 P→A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
362 Y→F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377.
377 Y→F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362.
400 R → C: lower Vmax and reduced Ki value for TBA inhibition of MPP(+); lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro); no change in transport efficiency (Vmax/Km) and clearance of salsolinol; dbSNP:rs8177516
432 K → Q: lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177517
458 Y→F: No change in TEA uptake.
544 Y→F: No change in TEA uptake.

Q8MJI6 Solute carrier family 22 member 2; Organic cation transporter 2 from Oryctolagus cuniculus (Rabbit) (see paper)
28% identity, 22% coverage: 71:179/489 of query aligns to 153:250/554 of Q8MJI6

query
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Sites not aligning to the query:

71 N→Q: Higher affinity for TEA. Loss of plasma membrane localization; when associated with Q-76. Loss of plasma membrane localization; when associated with Q-112. Loss of plasma membrane localization; when associated with Q-76 and Q-112.
96 N→Q: Higher affinity for TEA and lower Vmax.
112 N→Q: Higher affinity for TEA, lower Vmax and loss of plasma membrane localization.

8et9A Cryo-em structure of the organic cation transporter 2 in complex with 1-methyl-4-phenylpyridinium (see paper)
27% identity, 29% coverage: 40:179/489 of query aligns to 122:249/517 of 8et9A

query
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8et9A

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binding 1-methyl-4-phenylpyridin-1-ium: W216 (= W146), Q240 (= Q170), F243 (≠ S173)

Sites not aligning to the query:

binding 1-methyl-4-phenylpyridin-1-ium: 430

Query Sequence

>WP_101588932.1 NCBI__GCF_900169175.1:WP_101588932.1
MRGTVTAASAAEPSTARGPFRGKQRVLASAFVGTTIEWYDFYLYGTAAALVFNVQFFPMD
SELGSLLASFATLAVGFFARPLGGIIAGHLGDRVGRKALLVVSLVTMGIASTLIGLMPTY
EAIGWWAAVGLVVLRIFQGLSAGAEWGGSALLSVEHAPVGSRGLFGSFTQIGSAAGMLLA
TSAFFVVQSVLTDEQFAQFGWRIPFVASALLVVIGLWIRLGVSDAPEFLEHKESGNVRKQ
PLWEVVTRHPRVLLVTIGLRLAQNSVYYLITVYMLTYLADQRGDSTAGITAVMIASAIGL
VTGPFWGWVSDRLGRRTVTLGGIIGIGVFGWIFFAFLDNGPLILLPIIVVLGMNLAHDAV
YGPQAAWFAEQFPIEVRYSGVNMGYQLGTVIGGGIMPMVAALLFVAGGSTPWLICAYLTL
LCVLSGIAALAAHDPARAEALARRTDPGPAAAADSAEDPAADPAADSVATEVAAPAAADP
HRPIQETSH

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory