SitesBLAST
Comparing WP_102106154.1 NCBI__GCF_002869505.1:WP_102106154.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
58% identity, 97% coverage: 12:428/431 of query aligns to 6:421/429 of 6ed7A
- active site: Y17 (= Y23), Y144 (= Y151), D245 (= D252), K274 (= K281)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (= Y23), W52 (= W58), W52 (= W58), Y144 (= Y151), D147 (= D154), A217 (= A224), K274 (= K281), R391 (= R398), F393 (= F400), F393 (= F400)
- binding pyridoxal-5'-phosphate: G112 (= G118), S113 (= S119), Y144 (= Y151), H145 (= H152), D245 (= D252), I247 (= I254), K274 (= K281)
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
58% identity, 97% coverage: 12:428/431 of query aligns to 6:421/429 of P12995
- Y17 (= Y23) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W58) binding substrate
- GS 112:113 (= GS 118:119) binding pyridoxal 5'-phosphate
- Y144 (= Y151) mutation to F: Severely reduces the aminotransferase activity.
- D147 (= D154) mutation to N: Loss of aminotransferase activity.
- D245 (= D252) binding pyridoxal 5'-phosphate
- R253 (= R260) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K281) binding substrate; modified: N6-(pyridoxal phosphate)lysine
- G307 (= G314) binding substrate
- PT 308:309 (= PT 315:316) binding pyridoxal 5'-phosphate
- R391 (= R398) binding substrate; mutation to A: Reduces aminotransferase activity.
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
57% identity, 97% coverage: 12:428/431 of query aligns to 6:421/429 of 1dtyA
- active site: Y17 (= Y23), Y144 (= Y151), E211 (= E218), D245 (= D252), A248 (= A255), K274 (= K281), Y398 (= Y405)
- binding pyridoxal-5'-phosphate: G112 (= G118), S113 (= S119), Y144 (= Y151), H145 (= H152), D245 (= D252), I247 (= I254), K274 (= K281)
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
58% identity, 97% coverage: 12:428/431 of query aligns to 6:420/427 of 1mlzA
- active site: Y17 (= Y23), Y144 (= Y151), E210 (= E218), D244 (= D252), A247 (= A255), K273 (= K281), Y397 (= Y405)
- binding pyridoxal-5'-phosphate: G112 (= G118), S113 (= S119), Y144 (= Y151), H145 (= H152), D244 (= D252), I246 (= I254), K273 (= K281), P307 (= P315), T308 (= T316)
- binding trans-amiclenomycin: W52 (= W58), W53 (= W59), Y144 (= Y151), K273 (= K281), R390 (= R398), F392 (= F400)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
58% identity, 97% coverage: 12:428/431 of query aligns to 6:420/427 of 1mlyA
- active site: Y17 (= Y23), Y144 (= Y151), E210 (= E218), D244 (= D252), A247 (= A255), K273 (= K281), Y397 (= Y405)
- binding cis-amiclenomycin: W52 (= W58), W53 (= W59), K273 (= K281), R390 (= R398), F392 (= F400)
- binding pyridoxal-5'-phosphate: G112 (= G118), S113 (= S119), Y144 (= Y151), H145 (= H152), D244 (= D252), I246 (= I254), K273 (= K281), P307 (= P315), T308 (= T316)
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
57% identity, 97% coverage: 12:428/431 of query aligns to 6:409/416 of 1qj3A
- active site: Y17 (= Y23), Y144 (= Y151), E201 (= E218), D235 (= D252), A238 (= A255), K264 (= K281), Y386 (= Y405)
- binding 7-keto-8-aminopelargonic acid: Y17 (= Y23), W52 (= W58), Y144 (= Y151), K264 (= K281), R379 (= R398), F381 (= F400)
- binding pyridoxal-5'-phosphate: G112 (= G118), S113 (= S119), Y144 (= Y151), H145 (= H152), G146 (= G153), D235 (= D252), I237 (= I254), A238 (= A255), K264 (= K281)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
54% identity, 98% coverage: 9:431/431 of query aligns to 4:424/427 of 4cxqA
- active site: Y18 (= Y23), Y149 (= Y151), E212 (= E218), D246 (= D252), A249 (= A255), K275 (= K281), Y399 (= Y405)
- binding 7-keto-8-aminopelargonic acid: W56 (= W58), Y149 (= Y151), G308 (= G314), T310 (= T316), R392 (= R398)
- binding pyridoxal-5'-phosphate: G116 (= G118), S117 (= S119), Y149 (= Y151), H150 (= H152), G151 (= G153), E212 (= E218), D246 (= D252), I248 (= I254), K275 (= K281), P309 (= P315), T310 (= T316)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
54% identity, 98% coverage: 9:431/431 of query aligns to 4:422/425 of 4w1vA
- active site: Y18 (= Y23), Y147 (= Y151), E210 (= E218), D244 (= D252), A247 (= A255), K273 (= K281), Y397 (= Y405)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P22), Y18 (= Y23), W54 (= W58), M81 (= M85), G83 (= G87), Y147 (= Y151), G306 (= G314), P307 (= P315), T308 (= T316), F392 (= F400)
- binding pyridoxal-5'-phosphate: G114 (= G118), S115 (= S119), Y147 (= Y151), H148 (= H152), E210 (= E218), D244 (= D252), I246 (= I254), K273 (= K281)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
54% identity, 98% coverage: 9:431/431 of query aligns to 4:422/425 of 4cxrA
- active site: Y18 (= Y23), Y147 (= Y151), E210 (= E218), D244 (= D252), A247 (= A255), K273 (= K281), Y397 (= Y405)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (= Y23), W54 (= W58), W55 (= W59), A216 (= A224)
- binding pyridoxal-5'-phosphate: G114 (= G118), S115 (= S119), Y147 (= Y151), H148 (= H152), E210 (= E218), D244 (= D252), I246 (= I254), K273 (= K281), P307 (= P315), T308 (= T316)
P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
54% identity, 98% coverage: 9:431/431 of query aligns to 11:432/437 of P9WQ81
- Y25 (= Y23) mutation to A: Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
- W64 (= W58) binding S-adenosyl-L-methionine
- Y157 (= Y151) binding S-adenosyl-L-methionine
- K283 (= K281) modified: N6-(pyridoxal phosphate)lysine
- G316 (= G314) binding S-adenosyl-L-methionine
5te2A Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a mechanism-based inhibitor (see paper)
54% identity, 98% coverage: 9:431/431 of query aligns to 4:425/428 of 5te2A