SitesBLAST
Comparing WP_102938052.1 NCBI__GCF_002117405.1:WP_102938052.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
49% identity, 98% coverage: 10:479/481 of query aligns to 2:454/456 of 5oqtA
- binding alanine: I38 (= I46), G40 (= G48), T41 (≠ A49), G42 (= G50), F226 (= F251), A227 (= A252), I229 (= I254)
- binding : E24 (≠ A32), G26 (= G34), F28 (≠ A36), D29 (≠ S37), M32 (≠ A40), A176 (≠ S193), R177 (≠ F194), A184 (≠ V201), A188 (≠ M205), L192 (≠ V209), Q294 (≠ V319), V297 (≠ L322)
6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
49% identity, 98% coverage: 10:479/481 of query aligns to 4:456/458 of 6f34A
- binding arginine: I40 (= I46), G42 (= G48), T43 (≠ A49), G44 (= G50), E115 (= E121), Y116 (= Y122), A119 (≠ G125), F228 (= F251), A229 (= A252), I231 (= I254), V314 (≠ A337)
- binding cholesterol: W201 (≠ A216), Y202 (≠ H217)
- binding : G28 (= G34), F30 (≠ A36), D31 (≠ S37), M34 (≠ A40), A178 (≠ S193), R179 (≠ F194), A186 (≠ V201), I187 (= I202), A190 (≠ M205), L194 (≠ V209), Q296 (≠ V319), V299 (≠ L322)
P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
35% identity, 86% coverage: 12:424/481 of query aligns to 11:439/629 of P30825
- N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
24% identity, 95% coverage: 26:480/481 of query aligns to 15:447/461 of P76037
- Y110 (= Y122) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.
P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
26% identity, 92% coverage: 17:458/481 of query aligns to 1:442/489 of P25737
- M1 (≠ L17) modified: Initiator methionine, Removed
- Y102 (≠ L118) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- W106 (≠ Y122) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- K163 (≠ V200) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- F216 (= F251) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- E222 (≠ D257) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
- E230 (= E265) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
- D275 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
- D278 (≠ E303) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
- E438 (= E454) mutation to A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
Sites not aligning to the query:
- 443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
- 446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.
O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
26% identity, 95% coverage: 25:479/481 of query aligns to 4:436/438 of O34739
- C94 (≠ I114) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
- C141 (≠ T182) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
- C168 (≠ V209) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
- C291 (≠ Q345) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
- C415 (≠ L459) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.
Q7YQK4 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; L-type amino acid transporter 1; LAT1; Solute carrier family 7 member 5 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
23% identity, 94% coverage: 20:472/481 of query aligns to 33:470/503 of Q7YQK4
- C88 (≠ S74) mutation to S: No significant effect on inhibition by HgCl(2). Decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-183.
- C98 (= C84) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Slightly less decreased KM and Vmax for Phe; when associated with S-183.
- C160 (≠ I146) mutation to S: No change to KM or Vmax for Phe.
- C172 (≠ A158) mutation to S: No change to KM or Vmax for Phe.
- C174 (≠ A160) mutation to S: No change to KM or Vmax for Phe.
- C183 (≠ V169) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-88. Slightly less decreased KM and Vmax for Phe; when associated with S-98.
- G219 (≠ N222) mutation to D: Decreased KM and Vmax for Trp. Increased KM and Vmax for Phe; when associated with L-234.
- W234 (≠ F237) mutation to L: Decreased KM and Vmax for Trp. Increased KM but decreased Vmax for Phe. Increased KM and Vmax for Phe; when associated with D-219.
- C331 (≠ L334) mutation to S: No significant effect on inhibition by HgCl(2). Increased KM and Vmax for Phe.
- C377 (≠ G380) mutation to S: No significant effect on inhibition by HgCl(2).
- C403 (≠ A406) mutation to S: No significant effect on inhibition by HgCl(2).
- C439 (= C444) mutation to S: Prevents insertion into the plasma membrane and possibly protein folding.
- C454 (≠ T455) mutation to S: No significant effect on inhibition by HgCl(2). Slightly increased KM but slightly decreased Vmax for Phe.
Sites not aligning to the query:
- 492 C→S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe.
P82251 b(0,+)-type amino acid transporter 1; b(0,+)AT1; Glycoprotein-associated amino acid transporter b0,+AT1; Solute carrier family 7 member 9 from Homo sapiens (Human) (see 11 papers)
23% identity, 96% coverage: 11:473/481 of query aligns to 8:454/487 of P82251
- V40 (≠ I43) to M: in CSNU; uncertain significance
- IIGSG 43:47 (≠ IIGAG 46:50) binding L-arginine
- I44 (= I47) to T: in CSNU; type I; dbSNP:rs121908485
- S51 (≠ L54) to F: in CSNU; uncertain significance
- P52 (≠ T55) to L: in CSNU; impairs protein stability and dimer formation; dbSNP:rs1198613438
- A70 (≠ L72) to V: in CSNU; partial loss of amino acid transport activity; dbSNP:rs769448665
- Y99 (= Y101) to H: in CSNU; uncertain significance
- G105 (= G107) to R: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908480
- W114 (= W116) to R: in CSNU; uncertain significance
- I120 (≠ E121) to L: in CSNU; uncertain significance
- T123 (≠ A124) to M: in CSNU; partial loss of amino acid transport activity; dbSNP:rs79987078
- V142 (≠ L144) to A: no effect on amino acid transport activity; dbSNP:rs12150889
- C144 (≠ I146) modified: Interchain (with C-114 in SLC3A1)
- V170 (≠ T189) to M: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908479
- A182 (≠ V201) to T: in CSNU; type III; partial loss of amino acid transport activity; dbSNP:rs79389353
- G195 (= G214) to R: in CSNU; type III; decreased amino acid transport activity; dbSNP:rs121908482
- L223 (≠ V248) to M: slightly decreased amino acid transport activity; dbSNP:rs1007160
- A224 (≠ V249) to V: in CSNU; non-classic type I; dbSNP:rs140873167
- N227 (vs. gap) to D: in CSNU; decreased amino acid transport activity
- W230 (vs. gap) to R: in CSNU; complete loss of amino acid transport activity; mutation to A: Abolishes amino acid transport activity.
- D233 (≠ I254) binding L-arginine; mutation to A: Complete loss of amino acid transport activity.
- W235 (≠ F256) mutation to A: Complete loss of amino acid transport activity.
- Q237 (≠ G258) mutation to A: Reduces amino acid transport activity.
- G259 (≠ S280) to R: in CSNU; type III; impairs protein stability and dimer formation; dbSNP:rs121908483
- P261 (≠ V282) to L: in CSNU; types I and III; dbSNP:rs121908486
- S286 (≠ P307) to F: in CSNU; uncertain significance; dbSNP:rs755135545
- C321 (≠ L342) mutation to S: Does not affect amino acid transport activity.
- A324 (≠ Q345) to E: in CSNU; uncertain significance
- V330 (≠ A351) to M: in CSNU; type III; dbSNP:rs201618022
- A331 (≠ M352) to V: in CSNU; non-classic type I; dbSNP:rs768466784
- R333 (≠ Q354) to Q: in CSNU; decreased amino acid transport activity; dbSNP:rs769576205; to W: in CSNU; severe loss of amino acid transport activity; dbSNP:rs121908484
- A354 (≠ S375) to T: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs939028046
- S379 (= S400) mutation to A: Markedly reduces amino acid transport activity.
- A382 (≠ T403) to T: in CSNU; severe loss of amino acid transport activity; dbSNP:rs774878350
- W383 (≠ L404) mutation to A: Complete loss of amino acid transport activity.
- Y386 (≠ F407) mutation to A: Loss of amino acid transport activity.
- K401 (≠ P422) to E: in CSNU; uncertain significance; dbSNP:rs760264924
- L426 (= L445) to P: in CSNU; uncertain significance
Sites not aligning to the query:
- 482 P → L: in CSNU; severe loss of amino acid transport activity; no effect on localization to the apical membrane; dbSNP:rs146815072; mutation P->A,G,S,V: No effect on amino acid transport activity.; mutation P->F,I,M,W: Decreased amino acid transport activity.
6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
24% identity, 91% coverage: 43:479/481 of query aligns to 14:433/433 of 6f2wA
Q01650 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter from Homo sapiens (Human) (see 3 papers)
22% identity, 94% coverage: 23:472/481 of query aligns to 40:474/507 of Q01650