SitesBLAST
Comparing WP_103231942.1 NCBI__GCF_002899945.2:WP_103231942.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2qm1B Crystal structure of glucokinase from enterococcus faecalis
36% identity, 96% coverage: 12:321/322 of query aligns to 9:321/325 of 2qm1B
3vglA Crystal structure of a rok family glucokinase from streptomyces griseus in complex with glucose and amppnp (see paper)
34% identity, 95% coverage: 12:318/322 of query aligns to 4:308/312 of 3vglA
- binding phosphoaminophosphonic acid-adenylate ester: G9 (= G17), T11 (= T19), K12 (≠ N20), G130 (= G144), T131 (= T145), G180 (≠ A195), G214 (≠ S224), S218 (≠ Y228), G260 (= G270), V261 (= V271), E264 (≠ A274)
- binding beta-D-glucopyranose: G65 (≠ N79), P78 (= P91), N103 (= N117), D104 (= D118), L133 (≠ V147), G134 (= G148), E153 (= E167), H156 (= H170), E175 (= E190)
- binding zinc ion: H156 (= H170), C166 (≠ H180), C168 (≠ T183), C173 (≠ S188)
3vgkB Crystal structure of a rok family glucokinase from streptomyces griseus (see paper)
34% identity, 95% coverage: 12:318/322 of query aligns to 4:308/312 of 3vgkB
1z05A Crystal structure of the rok family transcriptional regulator, homolog of e.Coli mlc protein.
29% identity, 85% coverage: 30:304/322 of query aligns to 96:367/396 of 1z05A
5f7qE Rok repressor lmo0178 from listeria monocytogenes bound to operator (see paper)
27% identity, 80% coverage: 23:279/322 of query aligns to 96:346/396 of 5f7qE
Sites not aligning to the query:
- binding : 5, 8, 12, 15, 32, 43, 44, 67, 68, 68, 69, 69, 70, 70, 71, 72, 73
5f7rA Rok repressor lmo0178 from listeria monocytogenes bound to inducer (see paper)
26% identity, 80% coverage: 23:279/322 of query aligns to 15:262/306 of 5f7rA
- binding alpha-D-glucopyranose: G70 (≠ N79), N110 (≠ D118), N110 (≠ D118), S134 (≠ T142), V135 (≠ L143), G138 (= G146), L139 (≠ V147), G140 (= G148), E159 (= E167), H162 (= H170), E181 (= E190), E253 (≠ G270)
- binding zinc ion: H162 (= H170), C172 (≠ W181), C174 (≠ T183), C179 (≠ S188)
Sites not aligning to the query:
Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 26 papers)
31% identity, 80% coverage: 11:269/322 of query aligns to 409:669/722 of Q9Y223
- D413 (= D15) binding Mg(2+); to Y: in THC12; likely pathogenic; dbSNP:rs1280775456
- G416 (= G18) binding an N-acyl-D-mannosamine 6-phosphate
- T417 (= T19) binding ADP; to M: in THC12; likely pathogenic; dbSNP:rs1554659711
- N418 (= N20) binding ADP
- R420 (≠ K22) binding ADP; to Q: in THC12; likely pathogenic; dbSNP:rs780092539
- I472 (≠ V75) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity corresponding to less than 10% of wild-type activity
- G475 (≠ P78) to F: in THC12; likely pathogenic; requires 2 nucleotide substitutions
- G476 (vs. gap) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- R477 (≠ N79) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- V485 (≠ I87) to R: in THC12; uncertain significance; reduced protein abundance in homozygous patient cells; requires 2 nucleotide substitutions
- L486 (≠ E88) to P: in THC12; uncertain significance; dbSNP:rs774867424
- T489 (≠ P91) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- N516 (= N117) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- D517 (= D118) active site; binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
- N519 (= N120) to S: in NM and THC12; likely pathogenic; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910
- A524 (≠ G125) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
- F528 (= F129) to C: in NM; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986
- T544 (= T145) to R: in THC12; uncertain significance; reduced protein abundance in homozygous patient cells
- G545 (= G146) binding an N-acyl-D-mannosamine 6-phosphate
- C563 (≠ F164) to Y: in THC12; likely pathogenic; results in severely decreased cell surface sialylation
- E566 (= E167) binding an N-acyl-D-mannosamine
- H569 (= H170) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; binding Zn(2+)
- V572 (= V173) to L: in NM and THC12; likely pathogenic; mildly decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
- G576 (= G177) to E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
- C579 (≠ W181) binding Zn(2+)
- C581 (≠ T183) binding Zn(2+)
- C586 (≠ S188) binding Zn(2+)
- I587 (≠ L189) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603
- E588 (= E190) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- A630 (≠ C230) to T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
- A631 (= A231) to V: in NM; does not affect homohexamers formation; dbSNP:rs62541771
Sites not aligning to the query:
- 13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
- 19 binding UDP
- 23 binding UDP
- 113 binding UDP
- 132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation
- 157 H → Y: in THC12; likely pathogenic
- 176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
- 177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation; dbSNP:rs539332585
- 200 I → F: in NM; uncertain significance; dbSNP:rs369328625
- 206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
- 220 binding UDP
- 253 binding UDP
- 259 binding CMP-N-acetyl-beta-neuraminate
- 263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
- 266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
- 271 binding CMP-N-acetyl-beta-neuraminate
- 280 binding CMP-N-acetyl-beta-neuraminate
- 281 binding CMP-N-acetyl-beta-neuraminate
- 282 binding UDP
- 301 binding UDP
- 302 binding UDP
- 303 C → V: in NM; requires 2 nucleotide substitutions; dbSNP:rs121908633
- 307 binding UDP
- 321 binding UDP
- 331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
- 378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation; dbSNP:rs199877522
- 420:722 natural variant: Missing (in THC12; likely pathogenic)
- 704 P → R: in THC12; likely pathogenic; the orthologous mutation in mouse embryos results in cerebrospinal hemorrhages and defective angiogenesis; results in loss of cell surface sialylation
- 708 G → S: in NM and THC12; likely pathogenic; decreased UDP-N-acetylglucosamine 2-epimerase activity; severely decreased N-acylmannosamine kinase activity; dbSNP:rs1554657922
- 712 M → T: in NM; decreased N-acylmannosamine kinase activity; dbSNP:rs28937594
P50456 DNA-binding transcriptional repressor Mlc; Making large colonies protein; Membrane linked control from Escherichia coli (strain K12) (see 4 papers)
27% identity, 63% coverage: 99:300/322 of query aligns to 176:373/406 of P50456
- H247 (= H170) binding Zn(2+)
- C257 (≠ H180) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-259.; mutation to S: Strongly reduced activity; when associated with S-259.
- C259 (≠ T183) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-257.; mutation to S: Strongly reduced activity; when associated with S-257.
- C264 (≠ S188) binding Zn(2+)
- R306 (≠ K233) mutation to G: Forms dimers but not tetramers; when associated with G-310.
- L310 (≠ I237) mutation to G: Forms dimers but not tetramers; when associated with G-306.
Sites not aligning to the query:
- 52 R→H: Shows increased expression and forms larger colonies.
- 86 H→R: Can be bound and inactivated by MtfA.
- 136 F→A: Decreases association with PtsG EIIB domain.
1z6rA Crystal structure of mlc from escherichia coli (see paper)
28% identity, 62% coverage: 99:298/322 of query aligns to 152:347/382 of 1z6rA
O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
30% identity, 87% coverage: 11:291/322 of query aligns to 409:683/722 of O35826
- D413 (= D15) mutation D->K,N: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
- R420 (≠ K22) mutation to M: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
Sites not aligning to the query:
- 1 UDP-N-acetylglucosamine 2-epimerase
- 49 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Does not interfere with enzyme oligomerization.
- 110 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
- 132 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
- 155 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
- 157 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
- 406:722 N-acetylmannosamine kinase
2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
30% identity, 80% coverage: 11:269/322 of query aligns to 5:261/309 of 2yhwA
Q91WG8 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Mus musculus (Mouse) (see paper)
31% identity, 80% coverage: 11:269/322 of query aligns to 409:669/722 of Q91WG8
- C563 (≠ F164) mutation to Y: Loss-of-function mutant resulting in impaired sialic acid biosynthesis.
Sites not aligning to the query:
- 704 P→R: Homozygous embryos exhibit cerebrospinal hemorrhages and defective angiogenesis in the diencephalon at 11 dpc, and become non-viable between 11.5 and 12.5 dpc. Loss-of-function mutant resulting in impaired sialic acid biosynthesis.
2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
30% identity, 80% coverage: 11:269/322 of query aligns to 5:260/308 of 2yi1A
- binding adenosine-5'-diphosphate: G11 (= G17), T13 (= T19), N14 (= N20), R16 (≠ K22), T140 (= T145), G189 (≠ A195), L216 (≠ K225)
- binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G12 (= G18), G71 (≠ P78), G72 (vs. gap), R73 (≠ N79), S84 (≠ A90), T85 (≠ P91), L87 (= L93), N112 (= N117), D113 (= D118), G139 (= G144), T140 (= T145), G141 (= G146), I142 (≠ V147), E162 (= E167), H165 (= H170), E184 (= E190)
- binding calcium ion: N112 (= N117), N115 (= N120), G144 (≠ S149), A161 (≠ G166)
- binding zinc ion: H165 (= H170), C175 (≠ W181), C177 (≠ T183), C182 (≠ S188)
Sites not aligning to the query:
2yhyA Structure of n-acetylmannosamine kinase in complex with n- acetylmannosamine and adp (see paper)
30% identity, 80% coverage: 11:269/322 of query aligns to 5:260/308 of 2yhyA