SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_104485574.1 NCBI__GCF_002936955.1:WP_104485574.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
24% identity, 54% coverage: 12:513/933 of query aligns to 70:513/521 of Q8N465

query
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Q8N465

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S109 (≠ T51) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
N127 (≠ T69) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
G131 (≠ A73) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
I147 (≠ L87) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (≠ D93) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (≠ V112) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (≠ G129) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (= A145) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (= A171) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
G233 (= G173) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
D375 (= D366) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
R386 (= R377) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
T390 (≠ F381) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
V399 (= V394) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
K401 (≠ I396) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
R419 (≠ F417) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
A426 (≠ Y422) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
H434 (= H430) binding Zn(2+); mutation to A: Loss of catalytic activity.
G436 (≠ L432) to V: slight reduction in catalytic activity
N439 (= N435) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
H441 (= H437) binding Zn(2+); mutation to A: Loss of catalytic activity.
N443 (≠ V439) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
V444 (≠ F440) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
A446 (≠ Q442) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
E475 (= E475) binding Zn(2+); mutation to A: Loss of catalytic activity.
H476 (= H476) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
G477 (= G477) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
169:521 natural variant: Missing (in D2HGA1; uncertain significance)
400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
24% identity, 51% coverage: 42:513/933 of query aligns to 45:460/467 of 7qh2C

query
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I

I

K

K

A

Q

L

T

F

F

D

D

P

P

Q

K

G

N

I

L

L

L

N

N

P

P

binding flavin-adenine dinucleotide: V73 (≠ F70), G75 (≠ A72), S76 (≠ A73), G77 (= G74), T78 (= T75), G79 (≠ S76), L80 (= L77), A83 (≠ Q80), C84 (≠ A81), P137 (= P132), G138 (≠ A133), E139 (≠ S134), A142 (≠ C138), T143 (≠ K139), G146 (= G142), N147 (≠ I143), S149 (≠ A145), T150 (≠ N146), A152 (≠ S148), G153 (≠ S149), E203 (= E250), G204 (= G251), I209 (= I256), E422 (= E475), H423 (= H476)
binding fe (iii) ion: H377 (= H430), H384 (= H437), E422 (= E475)

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
24% identity, 55% coverage: 8:517/933 of query aligns to 5:455/455 of 8jdeA

query
sites
8jdeA

L

F

V

V

D

E

E

A

L

L

S

K

R

A

F

V

L

V

P

G

D

S

N

P

R

H

I

V

I

S

T

T

D

A

P

S

S

A

L

V

C

R

L

E

A

Q

Y

H

G

G

T

H

D

D

A

E

S

S

F

M

Y

H

R

R

L

C

L

Q

P

P

-

P

Q

D

L

A

V

V

L

V

R

W

L

P

A

Q

S

N

T

V

D

D

E

Q

V

V

V

S

R

R

V

V

L

A

Q

S

I

L

C

C

G

Y

E

N

H

Q

G

G

I

V

H

P

C

I

T

I

-
x
P

F

F

R
x
G

A
x
T

A
x
G

G
x
T

T
x
G

S

V

L

E

S

G

G
|
G

Q
x
V

-

C

A

A

V

V

S

Q

D

G

S

G

V

V

L

C

I

I

T

N

L
|
L

T

T

N

H

D

M

W

D

R

Q

H

I

H

T

E

E

V

L

L

N

D

T

D

E

G

D

H

F

R

S

I

V

A

V

L

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

D

A

A

L

N

N

K

T

Y

H

L

L

A

R

P

D

F

S

G

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

D

N

A

S

S

-

L

C

C

K
x
G

I
x
M

G

A

G
x
A

I
x
T

A

G

A
|
A

N
x
S

N

G

S

T

S

N

G

A

M

V

C

R

C

Y

G

G

T

T

A

M

Q

R

N

D

S

N

Y

-

R

-

T

-

L

V

A

I

G

N

M

L

S

E

L

V

V

V

L

L

A

P

D

D

G

G

S

R

L

L

D

H

T

T

R

-

D

-

E

-

Q

-

S

-

R

-

A

-

L

-

F

-

A

-

E

-

Q

-

K

-

P

-

E

-

L

-

A

-

G

-

L

-

R

-

Q

-

L

-

A

-

D

-

E

-

V

-

K

-

N

-

A

-

T

-

L

-

A

A

E

G

R

R

I

G

H

R

H

H

K

-

Y

Y

R

R

L

-

K

K

N

S

T

A

G

G

Y

Y

S

N

L

L

N

T

A

G

L

L

I

-

D

-

F

-

D

-

P

-

F

-

D

-

M

-

L

-

A

-

H

-

L

-

M

F

I

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

N

T

E

S

V

T

T

T

Y

L

-

R

-

L

H

H

T

P

V

A

P

P

D

E

H

A

A

T

F

V

K

A

A

A

S

T

C

C

L

-

V

A

Y

F

P

P

D

S

I

V

E

Q

T

A

C

V

L

D

A

S

V

T

T

V

E

Q

L

I

-

L

-

Q

A

A

N

A

T

V

Q

P

V

V

A

A

A

R

V

I

E

E

L

F

M

L

D

D

G

D

R

V

A

M

L

M

R

D

S

A

I

C

A

N

D

R

N

H

A

S

A

K

M

L

P

N

D

C

F

P

I

V

K

-

E

-

L

-

D

-

L

-

E

-

A

A

A

P

A

T

L

L

L

F

I

L

E

E

V

F

H

H

G

G

E

S

S

Q

E

-

A

-

Q

Q

L

T

L

L

A

A

R

E

C

Q

E

L

Q

Q

A

R

M

T

A

E

A

A

I

I

V

T

D

Q

F

D

A

N

R

G

L

G

N

S

E

H

V

F

P

S

F

W

T

A

Q

A

D

E

K

K

A

N

V

-

C

-

A

-

T

E

L

L

W

W

A

A

M

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

E

S

T

P

G

G

T

S

T

K

V

A

I

Y

I

S

E

T

D

D

V

V

A

C

F

V

H

P

V

I

E

S

Q

R

L

L

A

P

S

E

A

I

V

L

R

V

R

E

L

T

T

K

E

E

L

E

F

I

D

K

R

A

F

S

G

K

Y

L

D

T

E

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

L

G

A

D

G

G

N

N

L

F

H
|
H

F

C

V

I

F

L

T

L

Q

V

G

D

F

P

D

D

S

D

Q

A

A

E

E

E

L

Q

D

R

R

R

Y

V

G

K

G

A

F

F

M

A

D

E

A

N

V

L

A

G

Q

R

L

R

V

-

A

A

V

L

E

A

Y

L

Q

G

G

G

S

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

Y

L

V

L

E

Q

L

E

E

E

W

V

G

G

Q

P

D

V

G

G

Y

V

R

E

L

T

M

M

Q

R

R

Q

I

L

K

K

A

N

L

T

F

L

D

D

P

P

Q

R

G

G

I

L

L

M

N
|
N

P

P

G

G

V

K

I

V

L

L

binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ R71), T71 (≠ A72), G72 (≠ A73), T73 (≠ G74), G74 (≠ T75), G78 (= G79), V79 (≠ Q80), L90 (= L90), P132 (= P132), G133 (≠ A133), A134 (≠ S134), G140 (≠ K139), M141 (≠ I140), A143 (≠ G142), T144 (≠ I143), A146 (= A145), S147 (≠ N146), E200 (= E250), G201 (= G251), I206 (= I256), W322 (≠ F381), E413 (= E475), H414 (= H476), N450 (= N512)
binding lactic acid: R318 (= R377), H369 (= H430), H376 (= H437), H414 (= H476)
binding manganese (ii) ion: H369 (= H430), H376 (= H437), E413 (= E475)

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
24% identity, 55% coverage: 8:517/933 of query aligns to 5:456/456 of 8jdsA

query
sites
8jdsA

L

F

V

V

D

E

E

A

L

L

S

K

R

A

F

V

L

V

P

G

D

S

N

P

R

H

I

V

I

S

T

T

D

A

P

S

S

A

L

V

C

R

L

E

A

Q

Y

H

G

G

T

H

D

D

A
x
E

S

S

F

M

Y

H

R

R

L

C

L

Q

P

P

-

P

Q

D

L

A

V

V

L

V

R

W

L

P

A

Q

S

N

T

V

D

D

E

Q

V

V

V

S

R

R

V

V

L

A

Q

S

I

L

C

C

G

Y

E

N

H

Q

G

G

I

V

H

P

C

I

T

I

-
x
P

F

F

R
x
G

A
x
T

A
x
G

G
x
T

T
x
G

S

V

L

E

S

G

G
|
G

Q
x
V

-

C

A

A

V

V

S

Q

D

G

S

G

V

V

L

C

I

I

T

N

L
|
L

T

T

N

H

D

M

W

D

R

Q

H

I

H

T

E

E

V

L

L

N

D

T

D

E

G

D

H

F

R

S

I

V

A

V

L

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

D

A

A

L

N

N

K

T

Y

H

L

L

A

R

P

D

F

S

G

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

D

N

A

S

S

-

L

C

C

K
x
G

I
x
M

G

A

G
x
A

I
x
T

A

G

A
|
A

N
x
S

N

G

S

T

S

N

G

A

M

V

C

R

C

Y

G

G

T

T

A

M

Q

R

N

D

S

N

Y

-

R

-

T

-

L

V

A

I

G

N

M

L

S

E

L

V

V

V

L

L

A

P

D

D

G

G

S

R

L

L

D

H

T

T

R

-

D

-

E

-

Q

-

S

-

R

-

A

-

L

-

F

-

A

-

E

-

Q

-

K

-

P

-

E

-

L

-

A

-

G

-

L

-

R

-

Q

-

L

-

A

-

D

-

E

-

V

-

K

-

N

-

A

-

T

-

L

-

A

A

E

G

R

R

I

G

H

R

H

H

K

-

Y

Y

R

R

L

-

K

K

N

S

T

A

G

G

Y

Y

S

N

L

L

N

T

A

G

L

L

I

-

D

-

F

-

D

-

P

-

F

-

D

-

M

-

L

-

A

-

H

-

L

-

M

F

I

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

N

T

E

S

V

T

T

T

Y

L

-

R

-

L

H

H

T

P

V

A

P

P

D

E

H

A

A

T

F

V

K

A

A

A

S

T

C

C

L

-

V

A

Y

F

P

P

D

S

I

V

E

Q

T

A

C

V

L

D

A

S

V

T

T

V

E

Q

L

I

-

L

-

Q

A

A

N

A

T

V

Q

P

V

V

A

A

A

R

V

I

E

E

L

F

M

L

D

D

G

D

R

V

A

M

L

M

R

D

S

A

I

C

A

N

D

R

N

H

A

S

A

K

M

L

P

N

D

C

F

P

I

V

K

-

E

-

L

-

D

-

L

-

E

-

A

A

A

P

A

T

L

L

L

F

I

L

E

E

V

F

H

H

G

G

E

S

S

Q

E

-

A

-

Q

Q

L

T

L

L

A

A

R

E

C

Q

E

L

Q

Q

A

R

M

T

A

E

A

A

I

I

V

T

D

Q

F

D

A

N

R

G

L

G

N

S

E

H

V

F

P

S

F

W

T

A

Q

A

D

E

K

K

A

R

V

-

C

-

A

N

T

E

L

L

W

W

A

A

M

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

E

S

T

P

G

G

T

S

T

K

V

A

I

Y

I

S

E

T

D

D

V

V

A

C

F

V

H

P

V

I

E

S

Q

R

L

L

A

P

S

E

A

I

V

L

R

V

R

E

L

T

T

K

E

E

L

E

F

I

D

K

R

A

F

S

G

K

Y

L

D

T

E

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

L

G

A

D

G

G

N

N

L

F

H
|
H

F

C

V

I

F

L

T

L

Q

V

G

D

F

P

D

D

S

D

Q

A

A

E

E

E

L

Q

D

R

R

R

Y

V

G

K

G

A

F

F

M

A

D

E

A

N

V

L

A

G

Q

R

L

R

V

-

A

A

V

L

E

A

Y

L

Q

G

G

G

S

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

Y

L

V

L

E

Q

L

E

E

E

W

V

G

G

Q

P

D

V

G

G

Y

V

R

E

L

T

M

M

Q

R

R

Q

I

L

K

K

A

N

L

T

F

L

D

D

P

P

Q

R

G

G

I

L

L

M

N
|
N

P

P

G

G

V

K

I

V

L

L

binding flavin-adenine dinucleotide: E32 (≠ A35), P68 (vs. gap), G70 (≠ R71), T71 (≠ A72), G72 (≠ A73), T73 (≠ G74), G74 (≠ T75), G78 (= G79), V79 (≠ Q80), L90 (= L90), P132 (= P132), G133 (≠ A133), A134 (≠ S134), G140 (≠ K139), M141 (≠ I140), A143 (≠ G142), T144 (≠ I143), A146 (= A145), S147 (≠ N146), E200 (= E250), G201 (= G251), I206 (= I256), W323 (≠ F381), E414 (= E475), H415 (= H476), N451 (= N512)
binding manganese (ii) ion: H370 (= H430), H377 (= H437), E414 (= E475)
binding pyruvic acid: R319 (= R377), H370 (= H430), H377 (= H437), H415 (= H476)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
24% identity, 55% coverage: 8:517/933 of query aligns to 5:455/455 of 8jdtA

query
sites
8jdtA

L

F

V

V

D

E

E

A

L

L

S

K

R

A

F

V

L

V

P

G

D

S

N

P

R

H

I

V

I

S

T

T

D

A

P

S

S

A

L

V

C

R

L

E

A

Q

Y

H

G

G

T

H

D

D

A

E

S

S

F

M

Y

H

R

R

L

C

L

Q

P

P

-

P

Q

D

L

A

V

V

L

V

R

W

L

P

A

Q

S

N

T

V

D

D

E

Q

V

V

V

S

R

R

V

V

L

A

Q

S

I

L

C

C

G

Y

E

N

H

Q

G

G

I

V

H

P

C

I

T

I

-
x
P

F

F

R
x
G

A
x
T

A
x
G

G
x
T

T
x
G

S

V

L

E

S

G

G
|
G

Q
x
V

-

C

A

A

V

V

S

Q

D

G

S

G

V

V

L

C

I

I

T

N

L
|
L

T

T

N

H

D

M

W

D

R

Q

H

I

H

T

E

E

V

L

L

N

D

T

D

E

G

D

H

F

R

S

I

V

A

V

L

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

D

A

A

L

N

N

K

T

Y

H

L

L

A

R

P

D

F

S

G

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

D

N

A

S

S

-

L

C

C

K
x
G

I
x
M

G

A

G
x
A

I
x
T

A

G

A
|
A

N
x
S

N

G

S

T

S

N

G

A

M

V

C

R

C

Y

G

G

T

T

A

M

Q

R

N

D

S

N

Y

-

R

-

T

-

L

V

A

I

G

N

M

L

S

E

L

V

V

V

L

L

A

P

D

D

G

G

S

R

L

L

D

H

T

T

R

-

D

-

E

-

Q

-

S

-

R

-

A

-

L

-

F

-

A

-

E

-

Q

-

K

-

P

-

E

-

L

-

A

-

G

-

L

-

R

-

Q

-

L

-

A

-

D

-

E

-

V

-

K

-

N

-

A

-

T

-

L

-

A

A

E

G

R

R

I

G

H

R

H

H

K

-

Y

Y

R

R

L

-

K

K

N

S

T

A

G

G

Y

Y

S

N

L

L

N

T

A

G

L

L

I

-

D

-

F

-

D

-

P

-

F

-

D

-

M

-

L

-

A

-

H

-

L

-

M

F

I

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

N

T

E

S

V

T

T

T

Y

L

-

R

-

L

H

H

T

P

V

A

P

P

D

E

H

A

A

T

F

V

K

A

A

A

S

T

C

C

L

-

V

A

Y

F

P

P

D

S

I

V

E

Q

T

A

C

V

L

D

A

S

V

T

T

V

E

Q

L

I

-

L

-

Q

A

A

N

A

T

V

Q

P

V

V

A

A

A

R

V

I

E

E

L

F

M

L

D

D

G

D

R

V

A

M

L

M

R

D

S

A

I

C

A

N

D

R

N

H

A

S

A

K

M

L

P

N

D

C

F

P

I

V

K

-

E

-

L

-

D

-

L

-

E

-

A

A

A

P

A

T

L

L

L

F

I

L

E

E

V

F

H

H

G

G

E

S

S

Q

E

-

A

-

Q

Q

L

T

L

L

A

A

R

E

C

Q

E

L

Q

Q

A

R

M

T

A

E

A

A

I

I

V

T

D

Q

F

D

A

N

R

G

L

G

N

S

E

H

V

F

P

S

F

W

T

A

Q

E

D

K

K

R

A

N

V

-

C

-

A

-

T

E

L

L

W

W

A

A

M

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

E

S

T

P

G

G

T

S

T

K

V

A

I

Y

I

S

E

T

D

D

V

V

A

C

F

V

H

P

V

I

E

S

Q

R

L

L

A

P

S

E

A

I

V

L

R

V

R

E

L

T

T

K

E

E

L

E

F

I

D

K

R

A

F

S

G

K

Y

L

D

T

E

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

L

G

A

D

G

G

N

N

L

F

H
|
H

F

C

V

I

F

L

T

L

Q

V

G

D

F

P

D

D

S

D

Q

A

A

E

E

E

L

Q

D

R

R

R

Y

V

G

K

G

A

F

F

M

A

D

E

A

N

V

L

A

G

Q

R

L

R

V

-

A

A

V

L

E

A

Y

L

Q

G

G

G

S

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

Y

L

V

L

E

Q

L

E

E

E

W

V

G

G

Q

P

D

V

G

G

Y

V

R

E

L

T

M

M

Q

R

R

Q

I

L

K

K

A

N

L

T

F

L

D

D

P

P

Q

R

G

G

I

L

L

M

N
|
N

P

P

G

G

V

K

I

V

L

L

binding 2-ketobutyric acid: R318 (= R377), H369 (= H430), H376 (= H437), H414 (= H476)
binding flavin-adenine dinucleotide: P68 (vs. gap), G70 (≠ R71), T71 (≠ A72), G72 (≠ A73), T73 (≠ G74), G74 (≠ T75), G78 (= G79), V79 (≠ Q80), L90 (= L90), P132 (= P132), G133 (≠ A133), A134 (≠ S134), G140 (≠ K139), M141 (≠ I140), A143 (≠ G142), T144 (≠ I143), A146 (= A145), S147 (≠ N146), E200 (= E250), G201 (= G251), I206 (= I256), W322 (≠ F381), E413 (= E475), H414 (= H476), N450 (= N512)
binding manganese (ii) ion: H369 (= H430), H376 (= H437), E413 (= E475)

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
25% identity, 55% coverage: 8:517/933 of query aligns to 5:455/455 of 8jdxA

query
sites
8jdxA

L

F

V

V

D

E

E

A

L

L

S

K

R

A

F

V

L

V

P

G

D

S

N

P

R

H

I

V

I

S

T

T

D

A

P

S

S

A

L

V

C

R

L

E

A

Q

Y

H

G

G

T

H

D

D

A
x
E

S

S

F

M

Y

H

R

R

L

C

L

Q

P

P

-

P

Q

D

L

A

V

V

L

V

R

W

L

P

A

Q

S

N

T

V

D

D

E

Q

V

V

V

S

R

R

V

V

L

A

Q

S

I

L

C

C

G

Y

E

N

H

Q

G

G

I

V

H

P

C

I

T

I

-
x
P

F

F

R
x
G

A
x
T

A
x
G

G
x
T

T
x
G

S

V

L

E

S

G

G
|
G

Q
x
V

-

C

A

A

V

V

S

Q

D

G

S

G

V

V

L

C

I

I

T

N

L
|
L

T

T

N

H

D

M

W

D

R

Q

H

I

H

T

E

E

V

L

L

N

D

T

D

E

G

D

H

F

R

S

I

V

A

V

L

V

Q

E

P

P

G

G

V

V

I

T

G

R

A

K

D

A

A

L

N

N

K

T

Y

H

L

L

A

R

P

D

F

S

G

G

R

L

K

W

I

F

G

P

P

V

D

D

P
|
P

A
x
G

S
x
A

I

D

N

A

S

S

-

L

C

C

K
x
G

I
x
M

G

A

G
x
A

I
x
T

A

G

A
|
A

N
x
S

N

G

S

T

S

N

G

A

M

V

C

R

C

Y

G

G

T

T

A

M

Q

R

N

D

S

N

Y

-

R

-

T

-

L

V

A

I

G

N

M

L

S

E

L

V

V

V

L

L

A

P

D

D

G

G

S

R

L

L

D

H

T

T

R

-

D

-

E

-

Q

-

S

-

R

-

A

-

L

-

F

-

A

-

E

-

Q

-

K

-

P

-

E

-

L

-

A

-

G

-

L

-

R

-

Q

-

L

-

A

-

D

-

E

-

V

-

K

-

N

-

A

-

T

-

L

-

A

A

E

G

R

R

I

G

H

R

H

H

K

-

Y

Y

R

R

L

-

K

K

N

S

T

A

G

G

Y

Y

S

N

L

L

N

T

A

G

L

L

I

-

D

-

F

-

D

-

P

-

F

-

D

-

M

-

L

-

A

-

H

-

L

-

M

F

I

V

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

F

I

I
|
I

N

T

E

S

V

T

T

T

Y

L

-

R

-

L

H

H

T

P

V

A

P

P

D

E

H

A

A

T

F

V

K

A

A

A

S

T

C

C

L

-

V

A

Y

F

P

P

D

S

I

V

E

Q

T

A

C

V

L

D

A

S

V

T

T

V

E

Q

L

I

-

L

-

Q

A

A

N

A

T

V

Q

P

V

V

A

A

A

R

V

I

E

E

L

F

M

L

D

D

G

D

R

-

A

-

L

-

R

-

S

-

I

-

A

-

D

-

N

-

A

-

A

V

M

M

P

M

D

D

F

A

I

C

K

N

E

R

L

H

D

S

L

L

E

N

-

C

-

P

-

V

A

A

A

P

A

T

L

L

L

F

I

L

E

E

V

F

H

H

G

G

E

S

S

Q

E

-

A

-

Q

Q

L

T

L

L

A

A

R

E

C

Q

E

L

Q

Q

A

R

M

T

A

E

A

A

I

I

V

T

D

Q

F

D

A

N

R

G

L

G

N

S

E

H

V

F

P

S

F

W

T

A

Q

A

D

E

K

K

A

R

V

-

C

-

A

N

T

E

L

L

W

W

A

A

M

A

R
|
R

K

H

G

N

M

A

F
x
W

P

Y

A

A

V

A

G

L

A

A

V

L

R

-

E

S

T

P

G

G

T

S

T

K

V

A

I

Y

I

S

E

T

D

D

V

V

A

C

F

V

H

P

V

I

E

S

Q

R

L

L

A

P

S

E

A

I

V

L

R

V

R

E

L

T

T

K

E

E

L

E

F

I

D

K

R

A

F

S

G

K

Y

L

D

T

E

G

A

A

I

I

I

V

F

-

G

G

H
|
H

A

V

L

G

A

D

G

G

N

N

L

F

H
|
H

F

C

V

I

F

L

T

L

Q

V

G

D

F

P

D

D

S

D

Q

A

A

E

E

E

L

Q

D

R

R

R

Y

V

G

K

G

A

F

F

M

A

D

E

A

N

V

L

A

G

Q

R

L

R

V

-

A

A

V

L

E

A

Y

L

Q

G

G

G

S

T

L

C

K

T

A

G

E
|
E

H
|
H

G

G

T

I

G

G

R

L

N

G

M

K

A

R

P

Q

Y

L

V

L

E

Q

L

E

E

E

W

V

G

G

Q

P

D

V

G

G

Y

V

R

E

L

T

M

M

Q

R

R

Q

I

L

K

K

A

N

L

T

F

L

D

D

P

P

Q

R

G

G

I

L

L

M

N
|
N

P

P

G

G

V

K

I

V

L

L

binding flavin-adenine dinucleotide: E32 (≠ A35), P68 (vs. gap), G70 (≠ R71), T71 (≠ A72), G72 (≠ A73), T73 (≠ G74), G74 (≠ T75), G78 (= G79), V79 (≠ Q80), L90 (= L90), P132 (= P132), G133 (≠ A133), A134 (≠ S134), G140 (≠ K139), M141 (≠ I140), A143 (≠ G142), T144 (≠ I143), A146 (= A145), S147 (≠ N146), E200 (= E250), G201 (= G251), I206 (= I256), W322 (≠ F381), E413 (= E475), H414 (= H476), N450 (= N512)
binding 3-methyl-2-oxobutanoic acid: R318 (= R377), H369 (= H430), H376 (= H437), H414 (= H476)
binding manganese (ii) ion: H369 (= H430), H376 (= H437), E413 (= E475)

6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
24% identity, 54% coverage: 12:513/933 of query aligns to 18:461/467 of 6lpnB

query
sites
6lpnB

L

F

S

E

R

R

F

I

L

V

P

P

D

G

N

G

R

-

I

V

T

T

D

D

P

P

S

E

L

A

C

L

L

Q

A

A

Y

P

G

N

T

V

D

D

-
x
W

A

L

S

R

F

T

Y

L

R

R

L

G

L

C

P

S

Q

K

L

V

V

L

L

L

R

R

L

P

A

R

S

T

T

S

D

E

E

E

V

V

V

S

R

H

V

I

L

L

Q

R

I

H

C

C

G

H

E

E

H

R

G

N

I

L

H

A

C

V

T

N

F
x
P

R

Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

S

P

-

V

-

F

D

D

S

E

V

I

L

I

I

L

T

S

L

T

T

A

N

R

D

M

W

N

R

R

H

V

H

L

E

S

V

F

L

H

D

S

D

V

G

S

H

G

R

I

I

L

A

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

D

E

A

L

N

S

K

R

Y

Y

L

V

A

E

P

E

F

R

G

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A

G

S
x
A

I

K

N

G

S

S

C
|
C

K
x
H

I

I

G

G

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

S

A

S

G

G

G

M

L

C

R

C

F

G

L

T

R

A

Y

Q

G

N

S

S

L

Y

H

R

G

T

T

L

V

A

L

G

G

M

L

S

E

L

V

V

V

L

L

A

A

D

D

G

G

S

T

L

V

L

L

D

D

T

C

R

-

D

-

E

-

Q

-

S

-

R

-

A

-

L

-

F

-

A

-

E

-

Q

-

K

-

P

-

E

-

L

-

L

L

A

T

G

S

L

L

R

R

Q

-

L

-

A

-

D

-

E

-

V

-

K

-

N

-

A

-

T

-

L

-

A

-

E

-

R

-

I

-

H

-

K

-

Y

-

R

-

L

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

K

A

-

L

-

I

-

D

-

F

-

D

-

P

-

F

-

D

-

M

-

L

-

A

-

H

Q

L

L

M

F

I

I

G

G

S

S

E

E

G
|
G

T

T

L

L

G

G

F
x
I

I
|
I

N

T

E

T

V

V

T

S

Y

I

H

L

T

C

V

P

P

P

D

K

H

P

A

R

F

A

K

V

A

N

S

V

C

A

L

F

L

L

V

G

Y

C

P

P

-

G

-

F

-

A

D

E

I

V

E

L

T

Q

T

T

C

F

L

S

A

T

V

C

T

K

E

G

L

M

A

L

N

G

T

E

Q

I

V

L

A

S

A

A

V

F

E

E

L

F

M

M

D

D

G

A

R

V

A

C

L

M

R

Q

S

L

I

V

A

G

D

R

N

H

A

L

A

H

M

L

P

A

D

S

F

P

I

V

K

Q

E

E

L

-

D

-

L

-

E

S

A

P

A

F

A

Y

L

V

L

L

I

I

E

E

V

T

H

S

G

G

E

S

S

N

E

A

A

G

Q

H

L

D

L

A

A

E

R

K

C

L

E

G

Q

H

A

F

M

L

A

E

A

H

I

A

V

L

D

G

F

S

A

G

R

L

L

V

N

T

E

D

V

G

P

T

F

M

T

A

Q

T

D

D

K

Q

A

R

V

K

C

V

A

K

T

M

L

L

W

W

A

A

M

L

R

R

K

E

G

R

M

I

F

T

P

E

A

A

V

L

G

-

A

-

V

-

R

-

E

S

T

R

G

D

T

G

T

Y

V

V

I

Y

I

K

E

Y

D

D

V

L

A

S

F

L

H

P

V

V

E

E

Q

R

L

L

A

Y

S

D

A

I

V

V

R

-

L

-

T

T

E

D

L

L

F

R

D

A

R

R

F

L

G

G

-

P

-

H

Y

A

D

K

E

H

A

V

I

V

I

G

F

Y

G

G

H

H

A

L

L

G

A

D

G

G

N

N

L

L

H

H

F

L

V

N

F

V

T

T

Q

A

G

E

F

A

D

F

S

S

Q

P

A

S

E

L

L

L

D

-

R

-

Y

-

G

A

F

L

M

E

D

P

A

H

V

V

A

Y

Q

E

L

W

V

T

A

A

V

G

E

Q

Y

-

Q

Q

G

G

S

S

L

V

K

S

A

A

E
|
E

H

H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

Y

V

V

L

E

G

L

Y

E

S

W

K

G

P

Q

P

D

G

G

A

Y

L

R

Q

L

L

M

M

Q

Q

R

Q

I

L

K

K

A

A

L

L

F

L

D

D

P

P

Q

K

G

G

I

I

L

L

N
|
N

P

P

binding flavin-adenine dinucleotide: W40 (vs. gap), P76 (≠ F70), G78 (≠ A72), G79 (≠ A73), N80 (≠ G74), T81 (= T75), G82 (≠ S76), M83 (≠ L77), G86 (≠ Q80), S87 (≠ A81), L140 (≠ P132), A142 (≠ S134), C146 (= C138), H147 (≠ K139), G150 (= G142), N151 (≠ I143), A153 (= A145), T154 (≠ N146), G208 (= G251), I212 (≠ F255), I213 (= I256), E423 (= E475), N460 (= N512)

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
24% identity, 54% coverage: 12:513/933 of query aligns to 17:460/466 of 6lpxA

query
sites
6lpxA

L

F

S

E

R

R

F

I

L

V

P

P

D

G

N

G

R

-

I

V

T

T

D

D

P

P

S

E

L

A

C

L

L

Q

A

A

Y

P

G

N

T

V

D

D

-
x
W

A

L

S

R

F

T

Y

L

R

R

L

G

L

C

P

S

Q

K

L

V

V

L

L

L

R

R

L

P

A

R

S

T

T

S

D

E

E

E

V

V

V

S

R

H

V

I

L

L

Q

R

I

H

C

C

G

H

E

E

H

R

G

N

I

L

H

A

C

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

S

P

-

V

-

F

D

D

S

E

V

I

L

I

I

L

T

S

L

T

T

A

N

R

D

M

W

N

R

R

H

V

H

L

E

S

V

F

L

H

D

S

D

V

G

S

H

G

R

I

I

L

A

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

D

E

A

L

N

S

K

R

Y

Y

L

V

A

E

P

E

F

R

G

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

N

G

S

S

C
|
C

K

H

I

I

G

G

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

S

A

S

G

G
|
G

M

L

C

R

C

F

G

L

T

R

A

Y

Q

G

N

S

S

L

Y

H

R

G

T

T

L

V

A

L

G

G

M

L

S

E

L

V

V

V

L

L

A

A

D

D

G

G

S

T

L

V

L

L

D

D

T

C

R

-

D

-

E

-

Q

-

S

-

R

-

A

-

L

-

F

-

A

-

E

-

Q

-

K

-

P

-

E

-

L

-

L

L

A

T

G

S

L

L

R

R

Q

-

L

-

A

-

D

-

E

-

V

-

K

-

N

-

A

-

T

-

L

-

A

-

E

-

R

-

I

-

H

-

K

-

Y

-

R

-

L

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

K

A

-

L

-

I

-

D

-

F

-

D

-

P

-