SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_104485805.1 NCBI__GCF_002936955.1:WP_104485805.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
41% identity, 91% coverage: 12:412/442 of query aligns to 14:420/425 of O59010

query
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O59010

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S65 (= S69) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A273) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 273:275) binding L-aspartate
M311 (= M309) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T312) binding L-aspartate
V355 (= V353) binding L-aspartate
D394 (= D386) binding L-aspartate
M395 (= M387) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R389) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N393) binding L-aspartate
D405 (= D397) mutation to N: Strongly decreased affinity for aspartate.

2nwwA Crystal structure of gltph in complex with tboa (see paper)
40% identity, 90% coverage: 12:408/442 of query aligns to 5:407/407 of 2nwwA

query
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2nwwA

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binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S275), T305 (= T312), A349 (≠ V356), G350 (= G357), D385 (= D386), R388 (= R389), T389 (= T390), N392 (= N393)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
40% identity, 90% coverage: 12:411/442 of query aligns to 14:419/419 of 6x15A

query
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6x15A

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A

K

K

S

T

E

E

G

G

A

T

M

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ G50), F46 (≠ G50), P75 (≠ D79), L91 (≠ V96), F95 (≠ I100), L130 (= L134), I133 (≠ V137), I159 (≠ V163), Y167 (≠ L171), K196 (= K192), G200 (≠ L196), I207 (≠ Y203), F210 (= F206), L250 (≠ M246), I262 (≠ L259), M269 (≠ V266), T334 (= T332), V335 (≠ F333), G336 (≠ N334), T340 (≠ M338), L343 (≠ M341), M399 (≠ A391)
binding aspartic acid: S277 (= S274), S278 (= S275), T314 (= T312), G354 (= G352), A358 (≠ V356), G359 (= G357), D394 (= D386), R397 (= R389), T398 (= T390)
binding sodium ion: Y89 (= Y94), T92 (= T97), S93 (≠ T98), G306 (= G304), T308 (= T306), N310 (= N308), N310 (= N308), M311 (= M309), D312 (= D310), S349 (= S347), I350 (≠ V348), T352 (= T350), N401 (= N393), V402 (≠ I394), D405 (= D397)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
41% identity, 90% coverage: 12:409/442 of query aligns to 6:409/409 of 6bavA

query
sites
6bavA

S

Q

K

K

V

I

L

L

L

I

G

G

M

L

S

I

L

L

G

G

I

A

V

I

A

V

G

G

F

L

I

I

F

-

R

-

T

-

L

-

L

L

G

G

E

H

V

Y

A

G

F

Y

V

A

Q

D

E

A

Y

-

V

-

V

V

N

K

G

T

L

Y

L

V

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

F

V

A

S

S

L

L

V

V

C

V

G

G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

I

F

A

A

I

V

S

T

L

L

A

G

L

I

M

M

G

A

N

R

L

L

F

F

K

N

P

P

G

G

D

A

G

G

V

I

D

H

L

L

T

A

-

V

A

G

T

Q

F

F

A

Q

T

P

K

K

E

Q

A

A

P

P

S

P

L

L

G

V

D

K

V

I

I

L

V

L

G

D

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

G

S

A

M

L

A

A

Q

N

G

G

N

Q

T

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

S

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

D

M

A

F

I

G

N

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K

K

L

I

V

V

T

N

L

G

L

V

M

M

N

Q

V

Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

K

A

V

I

V

V

G

N

E

L

L

M

A

E

K

Y

V

F

T

L

A

V

A

L

V

A

Y

G

V

T

G

L

L

L

T

L

L

H

Q

G

I

L

L

V

L

T

V

Y

Y

S

F

I

V

M

L

F

L

R

K

A

I

F

Y

T

-

G

G

L

I

N

D

P

P

L

I

T

S

F

F

L

I

K

K

M

A

E

K

D

D

A

A

V

M

M

L

F

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E

R

T

V

A

A

T

-

R

K

R

E

M

M

G

G

V

I

D

S

N

E

K

G

V

I

A

Y

A

S

F

F

T

T

V

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

M
x
Y

Q

Q

G

G

V

V

A

C

T

T

A

F

F

F

I

I

A

A

Q

N

A

A

F

L

N

G

I

S

D

H

L

L

T

T

F

V

N

G

D

Q

Y

Q

L

L

M

T

V

I

I

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

V

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V

A

G

G

L

A

I

I

M

M

L

L

A

A

M

M

V

V

L

L

N

E

Q

S

V

V

G

G

L

L

P

P

V

L

-

T

-

D

-

P

-

N

-

V

-

A

E

A

G

A

I

Y

A

A

L

M

I

I

I

L

G

G

V

I

D
|
D

R

A

L

I

L

L

D
|
D

M

M

I

G

R
x
C

T
|
T

A

M

V

V

N

N

I

V

T

T

G

G

D

D

S

L

V

T

V

G

T

T

C

A

I

I

V

V

G

A

K

K

S

T

E

E

G

G

binding benzylcysteine: R268 (≠ A273), S270 (= S275), T306 (= T312), Y309 (≠ M315), D382 (= D382), D386 (= D386), C389 (≠ R389), T390 (= T390)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
41% identity, 90% coverage: 12:408/442 of query aligns to 6:408/408 of 6bauA

query
sites
6bauA

S

Q

K

K

V

I

L

L

L

I

G

G

M

L

S

I

L

L

G

G

I

A

V

I

A

V

G

G

F

L

I

I

F

-

R

-

T

-

L

-

L

L

G

G

E

H

V

Y

A

G

F

Y

V

A

Q

D

E

A

Y

-

V

-

V

V

N

K

G

T

L

Y

L

V

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

F

V

A

S

S

L

L

V

V

C

V

G

G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

I

F

A

A

I

V

S

T

L

L

A

G

L

I

M

M

G

A

N

R

L

L

F

F

K

N

P

P

G

G

D

A

G

G

V

I

D

H

L

L

T

A

-

V

A

G

T

Q

F

F

A

Q

T

P

K

K

E

Q

A

A

P

P

S

P

L

L

G

V

D

K

V

I

I

L

V

L

G

D

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

G

S

A

M

L

A

A

Q

N

G

G

N

Q

T

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

S

T

L

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

D

M

A

F

I

G

N

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K

K

L

I

V

V

T

N

L

G

L

V

M

M

N

Q

V

Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

K

A

V

I

V

V

G

N

E

L

L

M

A

E

K

Y

V

F

T

L

A

V

A

L

V

A

Y

G

V

T

G

L

L

L

T

L

L

H

Q

G

I

L

L

V

L

T

V

Y

Y

S

F

I

V

M

L

F

L

R

K

A

I

F

Y

T

-

G

G

L

I

N

D

P

P

L

I

T

S

F

F

L

I

K

K

M

A

E

K

D

D

A

A

V

M

M

L

F

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E

R

T

V

A

A

T

-

R

K

R

E

M

M

G

G

V

I

D

S

N

E

K

G

V

I

A

Y

A

S

F

F

T

T

V

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

C

T

T

A

F

F

F

I

I

A

A

Q

N

A

A

F

L

N

G

I

S

D

H

L

L

T

T

F

V

N

G

D

Q

Y

Q

L

L

M

T

V

I

I

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

V

I

G

G

T

T

A
|
A

G
|
G

V
|
V

P

P

G

G

V

A

G
|
G

L

A

I

I

M

M

L

L

A

A

M

M

V

V

L

L

N

E

Q

S

V

V

G

G

L

L

P

P

V

L

-

T

-

D

-

P

-

N

-

V

-

A

E

A

G

A

I

Y

A

A

L

M

I

I

I

L

G

G

V

I

D

D

R

A

L

I

L

L

D
|
D

M

M

I

G

R
x
C

T
|
T

A

M

V

V

N
|
N

I

V

T

T

G

G

D

D

S

L

V

T

V

G

T

T

C

A

I

I

V

V

G

A

K

K

S

T

E

E

binding cysteine: S270 (= S275), M303 (= M309), T306 (= T312), A345 (= A351), G346 (= G352), V347 (= V353), G351 (= G357), D386 (= D386), C389 (≠ R389), T390 (= T390), N393 (= N393)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
40% identity, 90% coverage: 12:408/442 of query aligns to 11:413/413 of 6x14A

query
sites
6x14A

S

Q

K

K

V
x
I

L

L

L

I

G

G

M

L

S

I

L

L

G

G

I

A

V

I

A

V

G

G

F

L

I

I

F

-

R

-

T

-

L

-

L

L

G

G

E

H

V

Y

A

G

F

Y

V

-

Q

-

E

A

Y

H

V

A

V

V

N

H

G

T

L

Y

L

V

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

C

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

F

V

A

S

S

L

L

V

V

C

V

G
|
G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L
x
V

A

V

F

Y

Y

Y

L

L

V

L

T

T

T

S

A

A

I

F

A

A

I

V

S

T

L

L

A

G

L

I

M

M

G

A

N

R

L

L

F

F

K

N

P

P

G

G

D

A

G

G

V

I

D

H

L

L

T

A

-

V

A

G

T

Q

F

F

A

Q

T

P

K

H

E

Q

A

A

P

P

S

P

L

L

G

V

D

H

V

I

I

L

V

L

G

D

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

G

S

A

M

L

A

A

Q

N

G

G

N

Q

T

V

L

L

Q

P

I

T

I

I

V

F

F

F

A

A

V

I

L
x
I

F

L

G

G

I

I

A

A

I

I

S

T

L
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

-

K

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

D

M

A

F

I

G

N

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K
|
K

L

I

V

V

T

N

L

G

L

V

M
|
M

N

Q

V

Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

H

A

V

I

V

V

G

N

E

L

L

M

A

E

K

Y

V

F

T

L

A

V

A

L

V

A

Y

G

V

T

G

L

L

L

T

L

L

H

Q

G

I

L

L

V

L

T

V

Y

Y

S

F

I

V

M

L

F

L

R

K

A

I

F

Y

T

-

G

G

L

I

N

D

P

P

L

I

T

S

F

F

L

I

K

K

K

H

M

A

E

K

D

D

A

A

V

M

M

L

F

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E

R

T

V

A

A

T

-

R

K

R

E

M

M

G

G

V

I

D

S

N

E

K

G

V

I

A

Y

A

S

F

F

T

T

V

L

P

P

L

L

G

G

A

A

T
|
T

I
|
I

N

N

M

M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

A

T

T

A

F

F

F

I

I

A

A

Q

N

A

A

F

L

N

G

I

S

D

H

L

L

T

T

F

V

N

G

D

Q

Y

Q

L

L

M

T

V

I

I

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

V
x
I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V

A

G
|
G

L

A

I

I

M

M

L

L

A

C

M

M

V

V

L

L

N

H

Q

S

V

V

G

G

L

L

P

P

V

L

-

T

-

D

-

P

-

N

-

V

-

A

E

A

G

A

I

Y

A

A

L

M

I

I

I
x
L

G

G

V

I

D

D

R

A

L

I

L

L

D
|
D

M

M

I

G

R
|
R

T

T

A

M

V

V

N

N

I

V

T

T

G

G

D

D

S

L

V

T

V

G

T

T

C

A

I

I

V

V

G

A

K

K

S

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G73), V83 (≠ L91), I157 (≠ L164), Y164 (≠ L171), K193 (= K192), T305 (= T306), I306 (= I307), I347 (≠ V348)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V14), M199 (= M198), S275 (= S275), T311 (= T312), G356 (= G357), L384 (≠ I379), D391 (= D386), R394 (= R389)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
41% identity, 91% coverage: 13:416/442 of query aligns to 11:422/425 of 6zgbA

query
sites
6zgbA

K

K

V

I

L

L

L

W

G

G

M

L

S

V

L

L

G

G

I

A

V

V

A

F

G

G

F

L

I

I

F

-

R

A

T

G

L

H

L

F

G

G

E

Y

V

A

A

G

F

A

V

V

Q

K

E

T

Y

Y

V

I

V

-

N

-

G

-

L

-

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

L

V

A

S

S

L

L

V

V

C

V

G

G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

V

A

T

T

T

S

A

A

I

M

A

A

I

V

S

F

L

F

A

G

L

L

L

I

M

V

G

G

N

R

L

L

F

F

K

N

P

V

G

G

D

A

G

N

V

V

D

N

L

L

T

G

A

S

A

G

T

T

T

G

F

K

A

A

T

I

K

E

-

A

E

Q

A

P

P

P

S

S

L

L

G

V

D

Q

V

T

I

L

V

L

G

N

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

A

S

S

M

L

A

A

Q

K

G

G

N

E

T

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

S

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

R

M

V

F

F

G

D

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K

L

L

I

V

V

T

G

L

G

L

V

M

M

N

Q

V

Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

R

A

V

I

V

V

G

N

P

L

L

M

A

E

K

Y

V

F

V

L

G

V

A

L

V

A

Y

G

T

T

G

L

L

L

F

L

L

H

Q

G

I

L

V

V

I

T

T

Y

Y

S

F

I

I

M

L

F

L

R

K

A

V

F

F

T

-

G

G

L

I

N

D

P

P

L

I

T

K

F

F

L

I

K

R

K

K

M

A

E

K

D

D

A

A

V

M

M

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E

R

T

V

A

A

T

E

R

E

M

M

G

G

V

V

D

D

N

K

K

G

V

I

A

F

A

S

F

F

T

T

V

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T

T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

A

L

F

F

I

V

A

A

Q

N

A

A

F

I

N

G

I

H

D

P

L

L

T

T

F

L

N

G

D

Q

Y

Q

L

L

M

V

V

V

I

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

V

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V

A

G

G

L

A

I

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

I

L

G

G

V

I

D

D

R

A

L

I

L

L

D
|
D

M

M

I

G

R
|
R

T
|
T

A

M

V

V

N

N

I

V

T

T

G

G

D

D

S

L

V

A

V

G

T

T

C

V

I

I

V

V

G

A

K

K

S

T

E

E

G

K

A

E

M

L

D

D

V

E

A

S

R

K

F

W

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A273), S273 (= S274), S274 (= S275), M308 (= M309), D392 (= D386), R395 (= R389), T396 (= T390)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
41% identity, 91% coverage: 13:416/442 of query aligns to 10:421/424 of 6zl4A

query
sites
6zl4A

K

K

V

I

L

L

L

W

G

G

M

L

S

V

L

L

G

G

I

A

V

V

A

F

G

G

F

L

I

I

F

-

R

A

T

G

L

H

L

F

G

G

E

Y

V

A

A

G

F

A

V

V

Q

K

E

T

Y

Y

V

I

V

-

N

-

G

-

L

-

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

L

V

A

S

S

L

L

V

V

C

V

G

G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

V

A

T

T

T

S

A

A

I

M

A

A

I

V

S

F

L

F

A

G

L

L

L

I

M

V

G

G

N

R

L

L

F

F

K

N

P

V

G

G

D

A

G

N

V

V

D

N

L

L

T

G

A

S

A

G

T

T

T

G

F

K

A

A

T

I

K

E

-

A

E

Q

A

P

P

P

S

S

L

L

G

V

D

Q

V

T

I

L

V

L

G

N

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

A

S

S

M

L

A

A

Q

K

G

G

N

E

T

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

S

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

R

M

V

F

F

G

D

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K
x
L

L

I

V

V

T

G

L
x
G

L

V

M

M

N

Q

V

Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

R

A

V

I

V

V

G

N

P

L

L

M

A

E

K

Y

V

F

V

L

G

V

A

L

V

A

Y

G

T

T

G

L

L

L

F

L

L

H

Q

G

I

L

V

V

I

T

T

Y

Y

S

F

I

I

M

L

F

L

R

K

A

V

F

F

T

-

G

G

L

I

N

D

P

P

L

I

T

K

F

F

L

I

K

R

K

K

M

A

E

K

D

D

A

A

V

M

M

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E
x
R

T

V

A

A

T

E

R

E

M

M

G

G

V

V

D

D

N

K

K

G

V

I

A

F

A

S

F

F

T

T

V

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

A

L

F

F

I

V

A

A

Q

N

A

A

F

I

N

G

I

H

D

P

L

L

T

T

F

L

N

G

D

Q

Y

Q

L

L

M

V

V

V

I

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

V

I

G

G

T

T

A

A

G

G

V

V

P

P

G
|
G

V
x
A

G

G

L

A

I

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

I

L

G

G

V

I

D

D

R

A

L

I

L

L

D

D

M

M

I

G

R
|
R

T
|
T

A

M

V

V

N

N

I

V

T

T

G

G

D

D

S

L

V

A

V

G

T

T

C

V

I

I

V

V

G

A

K

K

S

T

E

E

G

K

A

E

M

L

D

D

V

E

A

S

R

K

F

W

binding decyl-beta-d-maltopyranoside: L191 (≠ K192), G195 (≠ L196), R282 (≠ E284)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A273), S272 (= S274), S273 (= S275), M307 (= M309), T310 (= T312), G353 (= G355), A354 (≠ V356), R394 (= R389), T395 (= T390)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
41% identity, 91% coverage: 13:416/442 of query aligns to 13:424/427 of 5e9sA

query
sites
5e9sA

K

K

V

I

L

L

L

W

G

G

M

L

S

V

L

L

G

G

I

A

V

V

A

F

G

G

F

L

I

I

F

-

R

A

T

G

L

H

L

F

G

G

E

Y

V

A

A

G

F

A

V

V

Q

K

E

T

Y

Y

V

I

V

-

N

-

G

-

L

-

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

L

V

A

S

S

L

L

V

V

C

V

G

G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y
|
Y

L

L

V

A

T
|
T

T
x
S

A

A

I

M

A

A

I

V

S

F

L

F

A

G

L

L

L

I

M

V

G

G

N

R

L

L

F

F

K

N

P

V

G

G

D

A

G

N

V

V

D

N

L

L

T

G

A

S

A

G

T

T

T

G

F

K

A

A

T

I

K

E

-

A

E

Q

A

P

P

P

S

S

L

L

G

V

D

Q

V

T

I

L

V

L

G

N

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

A

S

S

M

L

A

A

Q

K

G

G

N

E

T

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

S

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

R

M

V

F

F

G

D

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K
x
L

L

I

V

V

T

G

L
x
G

L

V

M

M

N

Q

V
x
Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

R

A

V

I

V

V

G

N

P

L

L

M

A

E

K

Y

V

F

V

L

G

V

A

L

V

A

Y

G

T

T

G

L

L

L

F

L

L

H

Q

G

I

L

V

V

I

T

T

Y

Y

S

F

I

I

M

L

F

L

R

K

A

V

F

F

T

-

G

G

L

I

N

D

P

P

L

I

T

K

F

F

L

I

K

R

K

K

M

A

E

K

D

D

A

A

V

M

M

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E

R

T

V

A

A

T

E

R

E

M

M

G

G

V

V

D

D

N

K

K

G

V

I

A

F

A

S

F

F

T

T

V

L

P

P

L

L

G
|
G

A
|
A

T
|
T

I

I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

A

L

F

F

I

V

A

A

Q

N

A

A

F

I

N

G

I

H

D

P

L

L

T

T

F

L

N

G

D

Q

Y

Q

L

L

M

V

V

V

I

V

M

L

T

T

A

A

T

V

L

L

A

A

S
|
S

V
x
I

G
|
G

T
|
T

A

A

G
|
G

V
|
V

P

P

G

G

V
x
A

G
|
G

L

A

I

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

I

L

G

G

V

I

D

D

R

A

L

I

L

L

D
|
D

M

M

I

G

R
|
R

T
|
T

A

M

V

V

N
|
N

I
x
V

T

T

G

G

D
|
D

S

L

V

A

V

G

T

T

C

V

I

I

V

V

G

A

K

K

S

T

E

E

G

K

A

E

M

L

D

D

V

E

A

S

R

K

F

W

binding aspartic acid: R274 (≠ A273), S275 (= S274), S276 (= S275), T313 (= T312), G353 (= G352), V354 (= V353), A357 (≠ V356), G358 (= G357), D394 (= D386), R397 (= R389), T398 (= T390)
binding decyl-beta-d-maltopyranoside: L194 (≠ K192), G198 (≠ L196), Y202 (≠ V200)
binding sodium ion: Y87 (= Y94), T90 (= T97), S91 (≠ T98), S276 (= S275), G305 (= G304), A306 (= A305), T307 (= T306), N309 (= N308), N309 (= N308), M310 (= M309), D311 (= D310), S348 (= S347), I349 (≠ V348), G350 (= G349), T351 (= T350), N401 (= N393), V402 (≠ I394), D405 (= D397)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
41% identity, 91% coverage: 13:416/442 of query aligns to 13:424/426 of 6xwnB

query
sites
6xwnB

K

K

V

I

L

L

L

W

G

G

M

L

S

V

L

L

G

G

I

A

V

V

A

F

G

G

F

L

I

I

F

-

R

A

T

G

L

H

L

F

G

G

E

Y

V

A

A

G

F

A

V

V

Q

K

E

T

Y

Y

V

I

V

-

N

-

G

-

L

-

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

L

V

A

S

S

L

L

V

V

C

V

G

G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

V

A

T

T

T

S

A

A

I

M

A

A

I

V

S

F

L

F

A

G

L

L

L

I

M

V

G

G

N

R

L

L

F

F

K

N

P

V

G

G

D

A

G

N

V

V

D

N

L

L

T

G

A

S

A

G

T

T

T

G

F

K

A

A

T

I

K

E

-

A

E

Q

A

P

P

P

S

S

L

L

G

V

D

Q

V

T

I

L

V

L

G

N

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

A

S

S

M

L

A

A

Q

K

G

G

N

E

T

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

S

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

R

M

V

F

F

G

D

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K

L

L

I

V

V

T

G

L

G

L

V

M

M

N

Q

V

Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

R

A

V

I

V

V

G

N

P

L

L

M

A

E

K

Y

V

F

V

L

G

V

A

L

V

A

Y

G

T

T

G

L

L

L

F

L

L

H

Q

G

I

L

V

V

I

T

T

Y

Y

S

F

I

I

M

L

F

L

R

K

A

V

F

F

T

-

G

G

L

I

N

D

P

P

L

I

T

K

F

F

L

I

K

R

K

K

M

A

E

K

D

D

A

A

V

M

M

I

F

T

A

A

F

F

S

V

T

T

A

R

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E

R

T

V

A

A

T

E

R

E

M

M

G

G

V

V

D

D

N

K

K

G

V

I

A

F

A

S

F

F

T

T

V

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

A

L

F

F

I

V

A

A

Q

N

A

A

F

I

N

G

I

H

D

P

L

L

T

T

F

L

N

G

D

Q

Y

Q

L

L

M

V

V

V

I

V

M

L

T

T

A

A

T

V

L

L

A

A

S

S

V

I

G

G

T

T

A

A

G

G

V

V

P

P

G

G

V

A

G

G

L

A

I

I

M

M

L

L

A

A

M

M

V

V

L

L

N

Q

Q

S

V

V

G

G

L

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

V

V

E

A

-

L

G

A

I

Y

A

A

L

M

I

I

I

L

G

G

V

I

D

D

R

A

L

I

L

L

D
|
D

M

M

I

G

R
|
R

T

T

A

M

V

V

N

N

I

V

T

T

G

G

D

D

S

L

V

A

V

G

T

T

C

V

I

I

V

V

G

A

K

K

S

T

E

E

G

K

A

E

M

L

D

D

V

E

A

S

R

K

F

W

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S274), S276 (= S275), M310 (= M309), T313 (= T312), D394 (= D386), R397 (= R389)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
41% identity, 91% coverage: 13:416/442 of query aligns to 6:413/416 of 6r7rA

query
sites
6r7rA

K

K

V

I

L

L

L

W

G

G

M

L

S

V

L

L

G

G

I

A

V

V

A

F

G

G

F

L

I

I

F

-

R

A

T

G

L
x
H

L

F

G

G

E

Y

V

A

A

G

F

A

V

V

Q

K

E

T

Y

Y

V

I

V

-

N

-

G

-

L

-

Q

K

V

P

G

F

G

G

E

D

I

L

F

F

I

V

A

R

S

L

L

L

K

K

M

M

L

L

V

V

V

M

P

P

L

I

I

V

F

L

V

A

S

S

L

L

V

V

C

V

G

G

T

A

S

A

S

S

L

I

K

S

D

P

L

-

S

A

T

R

L

L

G

G

R

R

L

V

G

G

G

V

K

K

T

I

L

V

A

V

F

Y

Y

Y

L

L

V

A

T

T

T

S

A

A

I

M

A

A

I

V

S

F

L

F

A

G

L

L

L

I

M

V

G

G

N

R

L

L

F

F

K

N

P

V

G

G

D

A

G

N

V

V

D

N

L

L

T

G

A

S

A

G

T

T

T

G

F

-

A

-

T

-

K

A

E

Q

A

P

P

P

S

S

L

L

G

V

D

Q

V

T

I

L

V

L

G

N

M

I

F

V

P

P

T

T

N

N

P

P

I

F

N

A

S

S

M

L

A

A

Q

K

G

G

N

E

T

V

L

L

Q

P

I

V

I

I

V

F

F

F

A

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

S

T

L

Y

-

L

-

M

-

N

-

R

-

N

-

E

-

R

A

V

G

R

K

K

P

S

G

A

E

E

R

T

I

L

A

L

A

R

M

V

F

F

G

D

D

G

F

L

N

A

E

E

V

A

I

M

M

Y

K

L

L

I

V

V

T

G

L

G

L

V

M

M

N

Q

V

Y

A

A

P

P

Y

I

G

G

V

V

F

F

C

A

L

L

M

I

A

A

Q

Y

L

V

F

M

T

A

S

E

L

Q

G

G

L

V

D

R

A

V

I
x
V

V

G

N

P

L

L

M
x
A

E

K

Y

V

F

V

L

G

V

A

L

V

A

Y

G

T

T

G

L

L

L

F

L

L

H

Q

G

I

L

V

V

I

T

T

Y

Y

S

F

I

I

M

L

F

L

R

K

A

V

F

F

T

-

G

G

L

I

N

D

P

P

L

I

T

K

F

F

L

I

K

R

K

K

M

A

E

K

D

D

A

A

V

M

M

I

F

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

N

S

A

G

T

T

I

L

P

P

V

V

T

T

M

M

E

R

T

V

A

A

T

E

R

E

M

M

G

G

V

V

D

D

N

K

K

G

V

I

A

F

A

S

F

F

T

T

V

L

P

P

L

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

I

L

M

Y

Q

Q

G

G

V

V

A

T

T

V

A

L

F

F

I

V

A

A

Q

N

A

A

F

I

N

G

I

H