SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_104486353.1 NCBI__GCF_002936955.1:WP_104486353.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 95% coverage: 2:244/256 of query aligns to 11:255/265 of P07821

query
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P07821

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K50 (= K41) mutation to Q: Lack of activity.
D172 (= D161) mutation to E: Lack of activity.
E173 (= E162) mutation to A: Lack of activity.

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
35% identity, 86% coverage: 1:219/256 of query aligns to 5:211/353 of 1vciA

query
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1vciA

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binding adenosine-5'-triphosphate: F16 (≠ Y12), F19 (≠ G15), S41 (≠ N37), G42 (= G38), G44 (= G40), K45 (= K41), T46 (≠ S42), T47 (= T43)

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
37% identity, 79% coverage: 18:219/256 of query aligns to 22:225/375 of 2d62A

query
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binding pyrophosphate 2-: G42 (= G38), C43 (≠ T39), G44 (= G40), K45 (= K41), T46 (≠ S42), T47 (= T43)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
38% identity, 88% coverage: 3:226/256 of query aligns to 5:229/280 of 5x40A

query
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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y12), V18 (≠ L16), A20 (≠ I17), N40 (= N37), G41 (= G38), G43 (= G40), K44 (= K41), S45 (= S42), T46 (= T43), Q88 (≠ P82), H139 (≠ E135), M140 (≠ Q136), L141 (= L137), S142 (= S138), G144 (= G140), Q145 (= Q141), Q166 (≠ E162), H198 (= H195)
binding magnesium ion: S45 (= S42), Q88 (≠ P82)

3d31A Modbc from methanosarcina acetivorans (see paper)
34% identity, 88% coverage: 3:226/256 of query aligns to 2:217/348 of 3d31A

query
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Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

1g291 Malk (see paper)
33% identity, 79% coverage: 18:219/256 of query aligns to 19:222/372 of 1g291

query
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1g291

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E

D

A

N

I

I

L

A

M

F

A

P

L

L

K

K

V

L

H

R

G

-

K

K

V

V

R

P

V

R

T

Q

D

E

A

I

D

D

L

-

A

Q

L

R

V

V

R

R

G

E

V

V

L

A

D

E

E

L

L

L

E

G

I

L

A

T

H

E

L

L

S

L

P

N

R

R

Y

K

L

P

E

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

Q

Q

M

R

V

V

A

A

I

L

A

G

Q

R

A

A

L

I

I

V

R

R

R

K

P

P

K

Q

V

V

L

F

L

L

L

M

D

D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

Y

K

H

L

S

R

L

V

H

R

I

M

M

R

A

A

V

E

L

L

R

K

L

L

T

Q

R

R

E

Q

Q

L

N

G

M

V

V

T

T

T

L

I

V

Y

A

V

I

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

R

T

C

M

A

G

D

D

D

R

V

I

L

A

A

V

V

M

K

N

G

R

G

G

K

V

L

L

Q

Q

A

Q

F

V

G

G

binding magnesium ion: D69 (≠ N68), E71 (≠ S70), K72 (≠ R71), K79 (vs. gap), D80 (vs. gap)
binding pyrophosphate 2-: S38 (≠ N37), G39 (= G38), C40 (≠ T39), G41 (= G40), K42 (= K41), T43 (≠ S42), T44 (= T43)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
29% identity, 91% coverage: 3:234/256 of query aligns to 3:233/240 of 6mjpA

query
sites
6mjpA

L

L

T

K

L

A

E

Q

H

H

L

L

S

A

L

K

G

S

Y

Y

R

K

Q

K

G

R

L

K

I

V

V

V

D

S

D

D

L

V

S

S

L

L

T

Q

A

V

E

E

A

S

G

G

R

Q

L

I

S

V

V

G

L

L

G

G

P

P

N

N

G

G

T

A

G

G

K

K

S

T

T

T

L

S

L

F

K

Y

G

M

I

I

A

V

G

G

L

L

L

V

D

A

P

R

Q

D

Q

E

G

G

R

T

V

I

L

T

L

I

G

D

Q

N

D

D

L

I

A

S

N

I

L

L

-

P

-

M

-

H

S

S

R

R

A

S

R

R

R

M

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P

P

Q

Q

T

E

T

A

P

S

V

I

G

F

V

R

R

K

I

L

T

S

V

V

F

E

E

D

A

N

I

I

L

M

M

A

A

V

L

L

K

Q

V

T

H

R

G

E

K

E

V

L

R

T

V

H

T
x
E

D

E

A

R

D

Q

L
x
D

A

K

L

L

V

-

R

E

G

D

V

L

L

L

D

E

E

E

L

F

E

H

I

I

A

Q

H

H

L

I

S

R

P

K

R

S

Y

A

L

G

E

M

Q

A

L

L

S

S

G

G

Q

E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q

R

A

A

L

L

I

A

R

A

R

N

P

P

K

Q

V

F

L

I

L

L

D

D

E

Q

P

P

T

F

S

A

A

G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

K

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

R

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H

H

D

N

L

V

N

R

L

E

A

T

A

L

R

D

C

V

A

C

D

E

D

K

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

R

L

L

Q

I

A

A

F

E

G

G

P

T

L

P

H

Q

Q

D

C

V

L

L

Q

N

A

N

E

E

L

Q

I

V

Q

K

T

Q

L

V

Y

Y

binding calcium ion: E111 (≠ T110), D115 (≠ L114)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
30% identity, 91% coverage: 2:234/256 of query aligns to 2:233/238 of 6s8nB

query
sites
6s8nB

S

T

L

L

T

T

L

A

E

K

H

N

L

L

S

A

L

K

G

A

Y

Y

R

K

Q

G

G

R

L

R

I

V

V

V

D

E

D

D

L

V

S

S

L

L

T

T

A

V

E

N

A

S

G

G

R

E

L

I

S

V

V

G

L

L

G

G

P

P

N

N

G

G

T

A

G

G

K

K

S

T

T

T

L

T

L

F

K

Y

G

M

I

V

A

V

G

G

L

I

L

V

D

P

P

R

Q

D

Q

A

G

G

R

N

V

I

L

I

G

D

Q

D

D

D

L

I

A

S

N

L

L

L

-

P

-

L

-

H

S

A

R

R

A

A

R

R

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P

P

Q

Q

T

E

T

A

P

S

V

I

G

F

V
x
R

R

R

I

L

T

S

V

V

F

Y

E

D

A

N

I

L

L

M

M

A

A

V

L

L

K

Q

V

I

H

R

G

D

K

D

V

L

R

S

V

A

T

E

D

Q

A

R

D

E

L

-

A

D

L

R

V

A

R

N

G

E

V

L

L

M

D

E

E

E

L

F

E

H

I

I

A

E

H

H

L

L

S

R

P

D

R

S

Y
x
M

L
x
G

E
x
Q

Q

S

L

L

S

S

G

G

Q

E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q

R

A

A

L

L

I

A

R

A

R

N

P

P

K

K

V

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

R

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

S

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H

H

D

N

L

V

N

R

L

E

A

T

A

L

R

A

C

V

A

C

D

E

D

R

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

H

L

L

Q

I

A

A

F

H

G

G

P

T

L

P

H

T

Q

E

C

I

L

L

Q

Q

A

D

E

E

L

H

I

V

Q

K

T

R

L

V

Y

Y

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ V89), M133 (≠ Y133), G134 (≠ L134), Q135 (≠ E135)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
30% identity, 91% coverage: 2:234/256 of query aligns to 2:233/238 of 6s8gA

query
sites
6s8gA

S

T

L

L

T

T

L

A

E

K

H

N

L

L

S

A

L

K

G

A

Y
|
Y

R

K

Q

G

G
x
R

L

R

I

V

V

V

D

E

D

D

L

V

S

S

L

L

T

T

A

V

E

N

A

S

G

G

R

E

L

I

S

V

V

G

L

L

G

G

P

P

N
|
N

G

G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

K

Y

G

M

I

V

A

V

G

G

L

I

L

V

D

P

P

R

Q

D

Q

A

G

G

R

N

V

I

L

I

G

D

Q

D

D

D

L

I

A

S

N

L

L

L

-

P

-

L

-

H

S

A

R

R

A

A

R

R

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P

P

Q
|
Q

T

E

T

A

P

S

V

I

G

F

V

R

R

R

I

L

T

S

V

V

F

Y

E

D

A

N

I

L

L

M

M

A

A

V

L

L

K

Q

V

I

H

R

G

D

K

D

V

L

R

S

V

A

T

E

D

Q

A

R

D

E

L

-

A

D

L

R

V

A

R

N

G

E

V

L

L

M

D

E

E

E

L

F

E

H

I

I

A

E

H

H

L

L

S

R

P

D

R

S

Y

M

L

G

E

Q

Q
x
S

L

L

S
|
S

G

G

Q
x
E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q

R

A

A

L

L

I

A

R

A

R

N

P

P

K

K

V

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

R

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

S

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H

H

D

N

L

V

N

R

L

E

A

T

A

L

R

A

C

V

A

C

D

E

D

R

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

H

L

L

Q

I

A

A

F

H

G

G

P

T

L

P

H

T

Q

E

C

I

L

L

Q

Q

A

D

E

E

L

H

I

V

Q

K

T

R

L

V

Y

Y

binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y12), R15 (≠ G15), N37 (= N37), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43), Q84 (= Q83), S136 (≠ Q136), S138 (= S138), E141 (≠ Q141)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
30% identity, 91% coverage: 2:234/256 of query aligns to 2:233/234 of 6b89A

query
sites
6b89A

S

T

L

L

T

T

L

A

E

K

H

N

L

L

S

A

L

K

G

A

Y
|
Y

R

K

Q

G

G
x
R

L

R

I
x
V

V

V

D

E

D

D

L

V

S

S

L

L

T

T

A

V

E

N

A

S

G

G

R

E

L

I

S

V

V

G

L

L

G

G

P

P

N
|
N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

K

Y

G

M

I

V

A

V

G

G

L

I

L

V

D

P

P

R

Q

D

Q

A

G

G

R

N

V

I

L

I

G

D

Q

D

D

D

L

I

A

S

N

L

L

L

-

P

-
x
L

-
x
H

S

A

R

R

A

A

R

R

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P
|
P

Q
|
Q

T

E

T
x
A

P
x
S

V

I

G
x
F

V
x
R

R
|
R

I
x
L

T

S

V

V

F

Y

E

D

A

N

I

L

L

M

M

A

A
x
V

L

L

K

Q

V

I

H

R

G

D

K

D

V

L

R

S

V

A

T

E

D

Q

A

R

D

E

L

-

A

D

L

R

V

A

R

N

G

E

V

L

L

M

D

E

E

E

L

F

E

H

I

I

A

E

H

H

L

L

S

R

P

D

R

S

Y

M

L

G

E
x
Q

Q

S

L

L

S

S

G

G

Q

E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q
x
R

A

A

L

L

I

A

R

A

R

N

P

P

K

K

V

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

R

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

S

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H

H

D

N

L

V

N

R

L

E

A

T

A

L

R

A

C

V

A

C

D

E

D

R

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

H

L

L

Q

I

A

A

F

H

G

G

P

T

L

P

H

T

Q

E

C

I

L

L

Q

Q

A

D

E

E

L

H

I

V

Q

K

T

R

L

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y12), R15 (≠ G15), V17 (≠ I17), N37 (= N37), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43)
binding magnesium ion: T42 (≠ S42), Q84 (= Q83)
binding novobiocin: L71 (vs. gap), H72 (vs. gap), P83 (= P82), A86 (≠ T85), S87 (≠ P86), F89 (≠ G88), R90 (≠ V89), R91 (= R90), L92 (≠ I91), V101 (≠ A100), Q135 (≠ E135), R149 (≠ Q149)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
30% identity, 91% coverage: 2:234/256 of query aligns to 2:233/234 of 4p31A

query
sites
4p31A

S

T

L

L

T

T

L

A

E

K

H

N

L

L

S

A

L

K

G

A

Y
|
Y

R

K

Q

G

G
x
R

L

R

I
x
V

V

V

D

E

D

D

L

V

S

S

L

L

T

T

A

V

E

N

A

S

G

G

R

E

L

I

S

V

V

G

L

L

G

G

P

P

N

N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

K

Y

G

M

I

V

A

V

G

G

L

I

L

V

D

P

P

R

Q

D

Q

A

G

G

R

N

V

I

L

I

G

D

Q

D

D

D

L

I

A

S

N

L

L

L

-

P

-

L

-

H

S

A

R

R

A

A

R

R

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P

P

Q
|
Q

T

E

T

A

P

S

V

I

G

F

V

R

R

R

I

L

T

S

V

V

F

Y

E

D

A

N

I

L

L

M

M

A

A

V

L

L

K

Q

V

I

H

R

G

D

K

D

V

L

R

S

V

A

T

E

D

Q

A

R

D

E

L

-

A

D

L

R

V

A

R

N

G

E

V

L

L

M

D

E

E

E

L

F

E

H

I

I

A

E

H

H

L

L

S

R

P

D

R

S

Y

M

L

G

E

Q

Q

S

L

L

S

S

G

G

Q

E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q

R

A

A

L

L

I

A

R

A

R

N

P

P

K

K

V

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

R

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

S

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H

H

D

N

L

V

N

R

L

E

A

T

A

L

R

A

C

V

A

C

D

E

D

R

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

H

L

L

Q

I

A

A

F

H

G

G

P

T

L

P

H

T

Q

E

C

I

L

L

Q

Q

A

D

E

E

L

H

I

V

Q

K

T

R

L

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y12), R15 (≠ G15), V17 (≠ I17), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43)
binding magnesium ion: T42 (≠ S42), Q84 (= Q83)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
30% identity, 91% coverage: 2:234/256 of query aligns to 2:233/235 of 6mhzA

query
sites
6mhzA

S

T

L

L

T

T

L

A

E

K

H

N

L

L

S

A

L

K

G

A

Y
|
Y

R

K

Q

G

G

R

L

R

I

V

V

V

D

E

D

D

L

V

S

S

L

L

T

T

A

V

E

N

A

S

G

G

R

E

L

I

S

V

V

G

L

L

G

G

P

P

N
|
N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

K

Y

G

M

I

V

A

V

G

G

L

I

L

V

D

P

P

R

Q

D

Q

A

G

G

R

N

V

I

L

I

G

D

Q

D

D

D

L

I

A

S

N

L

L

L

-

P

-

L

-

H

S

A

R

R

A

A

R

R

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P

P

Q
|
Q

T

E

T

A

P

S

V

I

G

F

V

R

R

R

I

L

T

S

V

V

F

Y

E

D

A

N

I

L

L

M

M

A

A

V

L

L

K

Q

V

I

H

R

G

D

K

D

V

L

R

S

V

A

T

E

D

Q

A

R

D

E

L

-

A

D

L

R

V

A

R

N

G

E

V

L

L

M

D

E

E

E

L

F

E

H

I

I

A

E

H

H

L

L

S

R

P

D

R

S

Y

M

L

G

E

Q

Q
x
S

L

L

S
|
S

G
|
G

Q

E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q

R

A

A

L

L

I

A

R

A

R

N

P

P

K

K

V

F

L

I

L

L

D

D

E
|
E

P

P

T

F

S

A

A
x
G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

R

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

S

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H
|
H

D

N

L

V

N

R

L

E

A

T

A

L

R

A

C

V

A

C

D

E

D

R

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

H

L

L

Q

I

A

A

F

H

G

G

P

T

L

P

H

T

Q

E

C

I

L

L

Q

Q

A

D

E

E

L

H

I

V

Q

K

T

R

L

V

Y

Y

binding adp orthovanadate: Y12 (= Y12), N37 (= N37), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43), Q84 (= Q83), S136 (≠ Q136), S138 (= S138), G139 (= G139), G140 (= G140), E162 (= E162), G166 (≠ A166), H194 (= H195)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
30% identity, 91% coverage: 2:234/256 of query aligns to 2:233/233 of 6b8bA

query
sites
6b8bA

S

T

L

L

T

T

L

A

E

K

H

N

L

L

S

A

L

K

G

A

Y
|
Y

R

K

Q

G

G
x
R

L

R

I
x
V

V

V

D

E

D

D

L

V

S

S

L

L

T

T

A

V

E

N

A

S

G

G

R

E

L

I

S

V

V

G

L

L

G

G

P

P

N

N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

K

Y

G

M

I

V

A

V

G

G

L

I

L

V

D

P

P

R

Q

D

Q

A

G

G

R

N

V

I

L

I

G

D

Q

D

D

D

L

I

A

S

N

L

L

L

-

P

-

L

-

H

S

A

R

R

A

A

R

R

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P

P

Q

Q

T

E

T

A

P

S

V

I

G
x
F

V
x
R

R
|
R

I

L

T

S

V

V

F

Y

E

D

A

N

I

L

L

M

M

A

A

V

L

L

K

Q

V

I

H

R

G

D

K

D

V

L

R

S

V

A

T

E

D

Q

A

R

D

E

L

-

A

D

L

R

V

A

R

N

G

E

V

L

L

M

D

E

E

E

L

F

E

H

I

I

A

E

H

H

L

L

S

R

P

D

R

S

Y

M

L

G

E

Q

Q

S

L

L

S

S

G

G

Q

E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q

R

A

A

L

L

I

A

R

A

R

N

P

P

K

K

V

F

L

I

L

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

R

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

S

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H

H

D

N

L

V

N

R

L

E

A

T

A

L

R

A

C

V

A

C

D

E

D

R

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

H

L

L

Q

I

A

A

F

H

G

G

P

T

L

P

H

T

Q

E

C

I

L

L

Q

Q

A

D

E

E

L

H

I

V

Q

K

T

R

L

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (= Y12), R15 (≠ G15), V17 (≠ I17), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (≠ G88), R90 (≠ V89), R91 (= R90)

O65934 ABC transporter ATP-binding/permease protein Rv1747; EC 7.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
35% identity, 81% coverage: 17:223/256 of query aligns to 334:539/865 of O65934

query
sites
O65934

I

L

V

L

D

D

D

G

L

I

S

S

L

L

T

T

A

A

E

R

A

P

G

G

R

M

L

L

S

T

V

A

L

V

L

I

G

G

P

P

N

S

G

G

T

A

G

G

K

K

S

S

T

T

L

L

L

A

K

R

G

L

I

V

A

A

G

G

L

Y

L

T

D

H

P

P

Q

T

Q

D

G

G

R

T

V

V

L

T

L

F

G

E

G

G

Q

H

D

N

L

V

A

H

N

A

L

E

S

Y

R

A

A

S

R

L

R

R

A

S

G

-

Q

R

L

I

V

G

Y

M

L

V

P

P

Q

Q

T

D

P

V

V

V

G

H

V

G

R

Q

I

L

T

T

V

V

F

K

E

H

A

A

I

L

L

M

M

Y

A

A

L

A

K

E

V

L

H

R

G

L

K

P

V

P

R

D

V

T

T

T

D

K

A

D

D

D

-

R

L

T

A

Q

L

V

V

V

R

A

G

R

V

V

L

L

D

E

E

E

L

L

E

E

I

M

A

S

-

K

H

H

L

I

S

D

P

T

R

R

Y

-

L

V

E

D

Q

K

L

L

S

S

G

G

Q

Q

R

R

Q

K

M

R

V

A

A

S

I

V

A

A

Q

L

A

E

L

L

I

L

R

T

R

G

P

P

K

S

V

L

L

L

L

I

L

L

D

D

E
|
E

P

P

T

T

S

S

A

G

L

L

D

D

L

P

Y

A

H

L

S

D

L

R

H

Q

I

V

M

M

A

T

V

M

L

L

R

R

R

Q

L

L

T

A

R

-

E

D

Q

A

N

G

M

R

V

V

T

V

L

L

V

V

A

V

I

T

H

H

D

S

L

L

N

T

L

Y

A

L

A

D

R

V

C

C

A

D

D

Q

D

V

V

L

L

L

A

L

V

A

K

P

G

G

K

K

L

T

Q

A

A

F

F

C

G

G

P

P

L

P

H

T

Q

Q

E479 (= E162) mutation to Q: Loss of ATPase activity.

Sites not aligning to the query:

33 R→A: Strong decrease in phosphorylation.
47 S→A: Strong decrease in phosphorylation. Lack of interaction with PknF. Attenuates growth in macrophages.
69 N→A: Strong decrease in phosphorylation.
152 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-210.
210 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-152.
234 R→A: Strong decrease in phosphorylation.
248 S→A: Strong decrease in phosphorylation. Decreases interaction with PknF.
270 N→A: Strong decrease in phosphorylation.

6mbnA Lptb e163q in complex with atp (see paper)
29% identity, 91% coverage: 2:234/256 of query aligns to 3:234/241 of 6mbnA

query
sites
6mbnA

S

T

L

L

T

T

L

A

E

K

H

N

L

L

S

A

L

K

G

A

Y
|
Y

R

K

Q

G

G
x
R

L

R

I
x
V

V

V

D

E

D

D

L

V

S

S

L

L

T

T

A

V

E

N

A

S

G

G

R

E

L

I

S

V

V

G

L

L

G

G

P

P

N
|
N

G
|
G

T

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

K

Y

G

M

I

V

A

V

G

G

L

I

L

V

D

P

P

R

Q

D

Q

A

G

G

R

N

V

I

L

I

G

D

Q

D

D

D

L

I

A

S

N

L

L

L

-

P

-

L

-

H

S

A

R

R

A

A

R

R

A

G

G

-

Q

-

L

I

V

G

Y

Y

L

L

P

P

Q

Q

T

E

T

A

P

S

V

I

G

F

V

R

R

R

I

L

T

S

V

V

F

Y

E

D

A

N

I

L

L

M

M

A

A

V

L

L

K

Q

V

I

H

R

G

D

K

D

V

L

R

S

V

A

T

E

D

Q

A

R

D

E

L

-

A

D

L

R

V

A

R

N

G

E

V

L

L

M

D

E

E

E

L

F

E

H

I

I

A

E

H

H

L

L

S

R

P

D

R

S

Y

M

L

G

E

Q

Q

S

L

L

S

S

G

G

Q

E

R

R

Q

R

M

R

V

V

A

E

I

I

A

A

Q

R

A

A

L

L

I

A

R

A

R

N

P

P

K

K

V

F

L

I

L

L

D

D

E

Q

P

P

T

F

S

A

A

G

L

V

D

D

L

P

Y

I

H

S

S

V

L

I

H

D

I

I

M

K

A

R

V

I

L

I

R

E

R

H

L

L

T

-

R

R

E

D

Q

S

N

G

M

L

V

G

T

V

L

L

V

I

A

T

I

D

H
|
H

D

N

L

V

N

R

L

E

A

T

A

L

R

A

C

V

A

C

D

E

D

R

V

A

L

Y

A

I

V

V

K

S

G

Q

G

G

K

H

L

L

Q

I

A

A

F

H

G

G

P

T

L

P

H

T

Q

E

C

I

L

L

Q

Q

A

D

E

E

L

H

I

V

Q

K

T

R

L

V

Y

Y

binding adenosine-5'-triphosphate: Y13 (= Y12), R16 (≠ G15), V18 (≠ I17), N38 (= N37), G39 (= G38), G41 (= G40), K42 (= K41), T43 (≠ S42), T44 (= T43), H195 (= H195)

4fwiB Crystal structure of the nucleotide-binding domain of a dipeptide abc transporter (see paper)
32% identity, 91% coverage: 13:246/256 of query aligns to 16:263/310 of 4fwiB

query
sites
4fwiB

R

R

Q

E

G

G

L

T

I

I

-

K

-

A

V

A

D

D

D

G

L

I

S

S

L

L

T

D

A

I

E

L

A

E

G

N

R

S

L

V

S

T

V

A

L

I

L

V

G

G

P

E

N
x
S

G
x
A

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

I

K

E

G

A

I

M

A

T

G

K

L

T

L

L

D

P

P

P

Q
x
N

-

G

-

R

-

I

-

L

Q

S

G

G

R

R

V

V

L

L

L

Y

G

K

G

G

Q

K

D

D

L

L

A

L

N

T

L

M

S

R

-

E

-

L

R

R

A

K

R

I

R

R

A

W

G

K

Q

E

L

I

V

A

Y

L

L

V

P

P

Q
|
Q

T

A

P

Q

V

Q

G

S

V

L

R

N

I

P

T

T

-

M

-

K

V

V

F

I

E

E

A

H

I

F

L

K

M

D

A

T

L

V

K

E

V

A

H

H

G

G

K

-

V

V

R

R

V

W

T

S

D

H

A

S

D

E

L

L

A

-

L

-

V

I

R

E

G

K

V

A

L

S

D

E

E

K

L

L

E

R

I

M

A

V

H

R

L

L

S

N

P

P

R

E

-

A

-

V

-

L

-

N

-

S

Y

Y

L

P

E

L

Q

Q

L

L

S

S

G

G

Q

M

R

K

Q

Q

M

R

V

V

A

L

I

I

A

A

Q

L

A

A

L

L

I

L

R

L

R

D

P

P

K

V

V

V

L

L

L

I

L

L

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

L

V

Y

L

H

T

S

Q

L

A

H

H

I

I

M

I

A

Q

V

L

L

L

R

K

R

E

L

L

T

K

R

K

E

M

Q

L

N

K

M

I

V

T

T

L

L

I

V

F

A

V

I

T

H

H

D

D

L

I

N

A

L

V

A

A

R

E

C

L

A

A

D

D

D

K

V

V

L

A

A

V

V

I

K

Y

G

G

K

N

L

L

Q

V

A

E

F

Y

G

N

P

S

L

T

H

F

Q

Q

C

I

L

F

Q

K

A

N

E

P

L

L

I
x
H

Q
x
P

T

Y

L

T

Y

R

G

G

V

L

E

I

A

N

E

S

I

I

L

M

P

P

G

I

P

P

D

G

D

D

binding adenosine-5'-triphosphate: S42 (≠ N37), A43 (≠ G38), S44 (≠ T39), G45 (= G40), K46 (= K41), S47 (= S42), T48 (= T43), N60 (≠ Q55), Q96 (= Q83)
binding magnesium ion: S47 (= S42), Q96 (= Q83)
binding iron/sulfur cluster: H247 (≠ I230), P248 (≠ Q231)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13
binding iron/sulfur cluster: 274, 277, 280, 282, 287, 305, 306

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 79% coverage: 17:219/256 of query aligns to 32:230/378 of P69874

query
sites
P69874

I

V

V

I

D

P

D

Q

L

L

S

D

L

L

T

T

A

I

E

N

A

N

G

G

R

E

L
x
F

S

L

V

T

L

L

G

G

P

P

N

S

G

G

T
x
C

G

G

K

K

S

T

T

T

L

V

L
|
L

K

R

G

L

I

I

A

A

G

G

L

L

L

E

D

T

P

V

Q

D

Q

S

G

G

R

R

V

I

L

M

L
|
L

G

D

G

N

Q

E

D

D

L

I

A

T

N

H

L

V

S

P

R

A

A

E

R

N

R

R

A

Y

G

V

Q

N

L

T

V

V

Y

F

L

-

P

-

Q

Q

T

S

T

Y

P

A

V

L

G

F

V

P

R

H

I

M

T

T

V

V

F

F

E

E

A

N

I

V

L

A

M

F

A

G

L

L

K

R

V

M

H

Q

G

K

K

T

-

P

-

A

V

A

R

E

V

I

T

T

D

P

A

R

D

V

L

M

A

E

L

A

V

L

R

R

G

M

V
|
V

L

-

D

-

E

-

L

-

E

Q

I

L

A

E

H

T

L

F

S

A

P

Q

R

R

Y

K

L

P

E

H

Q

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

M

R

V

V

A

A

I

I

A

A

Q

R

A

A

L

V

I

V

R

N

R

K

P

P

K

R

V

L

L

L

D
|
D

E

E

P

S

T

L

S

S

A

A

L

L

D

D

L

Y

Y

K

H

L

S

R

L

K

H

Q

I

M

M

Q

A

N

V

E

L

L

R

K

R

A

L

L

T

Q

R

R

E

K

Q

L

N

G

M

I

V

T

T

F

L

V

V

F

A

V

I

T

H

H

D

D

L

Q

N

E

L

E

A

A

A

L

R

T

C

M

A

S

D

D

D

R

V

I

L

V

A

V

V

M

K

R

G

D

G

G

K

R

L

I

Q

E

A

Q

F

D

G

G

F45 (≠ L30) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ T39) mutation to T: Loss of ATPase activity and transport.
L60 (= L45) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L61) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V120) mutation to M: Loss of ATPase activity and transport.
D172 (= D161) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 85% coverage: 2:219/256 of query aligns to 3:216/371 of P68187

query
sites
P68187

S

S

L

V

T

Q

L

L

E

Q

H

N

L

V

S

T

L

K

G

A

Y

W

R

G

Q

E

G

V

L

V

I

V

V

S

D

K

D

D

L

I

S

N

L

L

T

D

A

I

E

H

A

E

G

G

R

E

L

F

S

V

V

V

L

F

L

V

G

G

P

P

N

S

G

G

T

C

G

G

K

K

S

S

T

T

L

L

K

R

G

M

I

I

A

A

G

G

L

L

L

E

D

T

P

I

Q

T

Q

S

G

G

R

D

V

L

L

F

L

I

G

G

G

E

Q

K

D

R

L

M

A

N

N

D

L

T

S

P

R

P

A

A

R

E

R

R

A

G

G

V

Q

G

L

M

V

V

Y

F

L

-

P

-

Q

Q

T

S

T

Y

P
x
A

V

L

G

Y

V

P

R

H

I

L

T

S

V

V

F

A

E

E

A

N

I

M

L

S

M

F

A

G

L

L

K

K

V

L

H

A

G

G

-

A

K
|
K

V

K

R

E

V

V

T

I

D

N

A

Q

D

R

L

-

A

-

L

-

V
|
V

R

N

G

Q

V
|
V

L

A

D
x
E

E

V

L

L

E

Q

I

L

A
|
A

H

H

L

L

S

L

P

D

R

R

Y

K

L

P

E

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

M

R

V

V

A

A

I

I

A

G

Q

R

A

T

L

L

I

V

R

A

R

E

P

P

K

S

V

V

L

F

L

L

D
|
D

E

E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

Y

A

H

L

S

R

L

V

H

Q

I

M

M

R

A

I

V

E

L

I

R

S

R

R

L

L

T

H

R

K

E

R

Q

L

N

G

M

R

V

T

T

M

L

I

V

Y

A

V

I

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

R

T

C

L

A

A

D

D

D

K

V

I

L

V

A

V

V

L

K

D

G

A

G

G

K

R

L

V

Q

A

A

Q

F

V

G

G

A85 (≠ P86) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (= K106) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V117) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V120) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ D122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
32% identity, 85% coverage: 2:219/256 of query aligns to 2:215/371 of 3puyA

query
sites
3puyA

S

S

L

V

T

Q

L

L

E

Q

H

N

L

V

S

T

L

K

G

A

Y
x
W

R

G

Q

E

G

V

L

V

I

V

V

S

D

K

D

D

L

I

S

N

L

L

T

D

A

I

E

H

A

E

G

G

R

E

L

F

S

V

V

V

L

F

L

V

G

G

P

P

N
x
S

G
|
G

T
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

G

M

I

I

A

A

G

G

L

L

L

E

D

T

P

I

Q

T

Q

S

G

G

R

D

V

L

L

F

L

I

G

G

G

E

Q

K

D

R

L

M

A

N

N

D

L

T

S

P

R

P

A

A

R

E

R

R

A

G

G

V

Q

G

L

M

V

V

Y

F

L

-

P

-

Q
|
Q

T

S

T

Y

P

A

V

L

G

Y

V

P

R

H

I

L

T

S

V

V

F

A

E

E

A

N

I

M

L

S

M

F

A

G

L

L

K

K

V

L

H

A

G

G

-

A

K

K

V

K

R

E

V

V

T

I

D

N

A

Q

D

R

L

-

A

-

L

-

V

V

R

N

G

Q

V

V

L

A

D

E

E

V

L

L

E

Q

I

L

A

A

H

H

L

L

S

L

P

D

R
|
R

Y

K

L

P

E

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

M

R

V

V

A

A

I

I

A

G

Q

R

A

T

L

L

I

V

R

A

R

E

P

P

K

S

V

V

L

F

L

L

D

D

E
|
E

P

P

T

L

S

S

A

N

L

L

D

D

L

A

Y

A

H

L

S

R

L

V

H

Q

I

M

M

R

A

I

V

E

L

I

R

S

R

R

L

L

T

H

R

K

E

R

Q

L

N

G

M

R

V

T

T

M

L

I

V

Y

A

V

I

T

H
|
H

D

D

L

Q

N

V

L

E

A

A

A

M

R

T

C

L

A

A

D

D

D

K

V

I

L

V

A

V

V

L

K

D

G

A

G

G

K

R

L

V

Q

A

A

Q

F

V

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y12), S37 (≠ N37), G38 (= G38), C39 (≠ T39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (= T43), Q81 (= Q83), R128 (= R132), A132 (≠ Q136), S134 (= S138), G136 (= G140), Q137 (= Q141), E158 (= E162), H191 (= H195)
binding magnesium ion: S42 (= S42), Q81 (= Q83)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
32% identity, 85% coverage: 2:219/256 of query aligns to 2:215/371 of 3puxA

query
sites
3puxA

S

S

L

V

T

Q

L

L

E

Q

H

N

L

V

S

T

L

K

G

A

Y
x
W

R

G

Q

E

G

V

L

V

I

V

V

S

D

K

D

D

L

I

S

N

L

L

T

D

A

I

E

H

A

E

G

G

R

E

L

F

S

V

V

V

L

F

L

V

G

G

P

P

N
x
S

G
|
G

T
x
C

G
|
G

K
|
K

S
|
S