SitesBLAST
Comparing WP_106709268.1 NCBI__GCF_003010955.1:WP_106709268.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 47% coverage: 4:191/403 of query aligns to 49:228/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (= V28), G75 (= G30), S76 (≠ Y31), G77 (= G32), T78 (≠ S33), G79 (≠ K34), L80 (≠ R35), A83 (≠ R39), C84 (≠ P40), P137 (≠ Y94), G138 (= G95), E139 (≠ P96), A142 (≠ G105), T143 (≠ S106), G146 (= G109), N147 (≠ L110), S149 (≠ A112), T150 (= T113), A152 (≠ L115), G153 (≠ C116), E203 (≠ W166), G204 (= G167), I209 (≠ A172)
Sites not aligning to the query:
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 44% coverage: 6:181/403 of query aligns to 46:213/459 of P9WIT1
Sites not aligning to the query:
- 354 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
28% identity, 52% coverage: 3:210/403 of query aligns to 44:244/465 of 3pm9A
- active site: A149 (≠ L115), L159 (≠ S125)
- binding flavin-adenine dinucleotide: P69 (≠ G30), Q70 (≠ Y31), G71 (= G32), G72 (≠ S33), N73 (≠ K34), T74 (≠ R35), G75 (≠ D36), L76 (= L37), G79 (vs. gap), Q80 (≠ G38), L91 (= L49), L133 (≠ Y94), G134 (= G95), A135 (≠ P96), C139 (≠ G105), T140 (≠ S106), G142 (= G108), G143 (= G109), S146 (≠ A112), T147 (= T113), A149 (≠ L115), G150 (≠ C116), E200 (≠ W166), G201 (= G167), I205 (= I171), I206 (≠ A172)
Sites not aligning to the query:
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
26% identity, 83% coverage: 64:396/403 of query aligns to 112:460/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R282), T337 (≠ P286), K348 (≠ R297), Y379 (≠ F321), H381 (≠ D323), H388 (≠ W330), H423 (≠ A361)
- binding flavin-adenine dinucleotide: L139 (≠ Y94), G140 (= G95), A141 (≠ P96), C145 (≠ G105), G149 (= G109), N150 (≠ L110), A152 (= A112), T153 (= T113), G157 (= G117), G207 (= G167), I212 (≠ A172), E422 (≠ P360), N459 (= N395)
- binding zinc ion: H381 (≠ D323), H388 (≠ W330), E422 (≠ P360)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 39, 75, 76, 77, 78, 79, 80, 81, 82, 85, 86
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
26% identity, 83% coverage: 64:396/403 of query aligns to 112:460/466 of 6lpuA
- binding flavin-adenine dinucleotide: L139 (≠ Y94), G140 (= G95), A141 (≠ P96), C145 (≠ G105), H146 (≠ S106), G148 (= G108), G149 (= G109), N150 (≠ L110), A152 (= A112), T153 (= T113), A155 (≠ L115), E206 (≠ W166), G207 (= G167), I211 (= I171), I212 (≠ A172), E422 (≠ P360), N459 (= N395)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R282), T337 (≠ P286), K348 (≠ R297), Y379 (≠ F321), H381 (≠ D323), H388 (≠ W330), H423 (≠ A361)
- binding zinc ion: H381 (≠ D323), H388 (≠ W330), E422 (≠ P360)
Sites not aligning to the query:
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
26% identity, 83% coverage: 64:396/403 of query aligns to 112:460/466 of 6lpqA
- binding flavin-adenine dinucleotide: L139 (≠ Y94), G140 (= G95), A141 (≠ P96), C145 (≠ G105), H146 (≠ S106), G149 (= G109), N150 (≠ L110), A152 (= A112), T153 (= T113), A155 (≠ L115), G157 (= G117), E206 (≠ W166), G207 (= G167), I211 (= I171), I212 (≠ A172), E422 (≠ P360), N459 (= N395)
- binding d-malate: R333 (= R282), T337 (≠ P286), K348 (≠ R297), Y379 (≠ F321), H381 (≠ D323), H388 (≠ W330), E422 (≠ P360), H423 (≠ A361)
- binding zinc ion: H381 (≠ D323), H388 (≠ W330), E422 (≠ P360)
Sites not aligning to the query:
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
26% identity, 83% coverage: 64:396/403 of query aligns to 112:460/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R282), T337 (≠ P286), K348 (≠ R297), Y379 (≠ F321), H381 (≠ D323), H388 (≠ W330), N390 (≠ R332), H423 (≠ A361)
- binding flavin-adenine dinucleotide: L139 (≠ Y94), G140 (= G95), A141 (≠ P96), C145 (≠ G105), G149 (= G109), N150 (≠ L110), A152 (= A112), T153 (= T113), A155 (≠ L115), G157 (= G117), G207 (= G167), I212 (≠ A172), E422 (≠ P360), H423 (≠ A361)
- binding zinc ion: H381 (≠ D323), H388 (≠ W330), E422 (≠ P360)
Sites not aligning to the query:
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
26% identity, 83% coverage: 64:396/403 of query aligns to 113:461/467 of 6lpnB
- binding flavin-adenine dinucleotide: L140 (≠ Y94), A142 (≠ P96), C146 (≠ G105), H147 (≠ S106), G150 (= G109), N151 (≠ L110), A153 (= A112), T154 (= T113), G208 (= G167), I212 (= I171), I213 (≠ A172), E423 (≠ P360), N460 (= N395)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
29% identity, 43% coverage: 64:236/403 of query aligns to 165:329/521 of Q8N465
- C172 (≠ T71) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P91) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A112) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ G138) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G140) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 109 S → W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- 127 N → K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- 131 G → V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- 147 I → S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- 153 M → T: in D2HGA1; uncertain significance; significant loss of catalytic activity; M → V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 375 D → Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- 386 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; R→A: Loss of catalytic activity.
- 390 binding (R)-2-hydroxyglutarate; binding (R)-malate; T→A: Significantly reduced catalytic activity.
- 399 V → M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
- 401 binding (R)-2-hydroxyglutarate; binding (R)-malate; K→A: Loss of catalytic activity.
- 419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- 426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- 434 binding Zn(2+); H→A: Loss of catalytic activity.
- 436 G → V: slight reduction in catalytic activity
- 439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- 441 binding Zn(2+); H→A: Loss of catalytic activity.
- 443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
- 444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- 446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- 475 binding Zn(2+); E→A: Loss of catalytic activity.
- 476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
- 477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/455 of 8jdpA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 369, 413, 450
- binding deaminohydroxyvaline: 319, 414
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/455 of 8jdtA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding 2-ketobutyric acid: 318, 369, 376, 414
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
- binding manganese (ii) ion: 369, 376, 413
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/455 of 8jdeA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
- binding lactic acid: 318, 369, 376, 414
- binding manganese (ii) ion: 369, 376, 413
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/455 of 8jdxA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
- binding 3-methyl-2-oxobutanoic acid: 318, 369, 376, 414
- binding manganese (ii) ion: 369, 376, 413
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/456 of 8jdsA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 323, 414, 415, 451
- binding manganese (ii) ion: 370, 377, 414
- binding pyruvic acid: 319, 370, 377, 415
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/454 of 8jdzA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding (3S)-3-methyl-2-oxopentanoic acid: 318, 322, 369, 376, 413
- binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 322, 412, 413, 449
- binding manganese (ii) ion: 369, 376, 412
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/454 of 8jdyA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding 2-oxo-4-methylpentanoic acid: 318, 322, 336, 369, 376, 413
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 412, 449
- binding manganese (ii) ion: 369, 376, 412
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/455 of 8jduA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding 2-oxopentanoic acid: 318, 322, 369, 376, 414
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 450
- binding manganese (ii) ion: 369, 376, 413
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/456 of 8jdrA
- binding flavin-adenine dinucleotide: L90 (= L49), P132 (= P101), G133 (≠ A102), A134 (≠ G103), G140 (= G109), M141 (≠ L110), A143 (= A112), T144 (= T113), A146 (≠ L115), S147 (≠ C116), E200 (≠ W166), G201 (= G167), I206 (≠ A172)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 324, 370, 414, 451
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: 319, 323, 415
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
30% identity, 34% coverage: 46:184/403 of query aligns to 87:218/456 of 8jdqA