SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_106710428.1 NCBI__GCF_003010955.1:WP_106710428.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

B0SR19 Sugar transporter SemiSWEET from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (see paper)
43% identity, 63% coverage: 48:127/127 of query aligns to 3:82/85 of B0SR19

query
sites
B0SR19

E

N

L

L

I

I

G

G

S

Y

L

V

A

A

A

A

V

F

I

L

T

T

T

T

L

V

C

S

W

F

V

L

P

P

Q

Q

I

V

I

L

K

R

I

V

V

V

R

M

H

T

R

K

E

Q

T

T

A

R

S

D

I

I

S

S

L

R

V

N

M

M

N

Y

V

I

S

M

L

F

V

F

L

L

G

G

V

V

F

V

L

L

W
|
W

F

F

I

V

Y

Y

G

G

M

I

M

L

I

R

G

S

S

D

W

L

P

P

V

I

I

I

A

L

A

A

N
|
N

G

V

V

V

T

T

L

L

L

F

L

F

I

V

L

T

T

I

I

I

L

L

G

Y

L

Y

K

K

L

L

R

T

Y

E

G

G

W48 (= W93) mutation to A: Impairs glucose transport.
N64 (= N109) mutation to A: Impairs glucose transport.

5uhsA Structure of a semisweet d57a mutant (see paper)
43% identity, 61% coverage: 48:124/127 of query aligns to 3:79/81 of 5uhsA

query
sites
5uhsA

E

N

L

L

I

I

G

G

S

Y

L

V

A

A

A

A

V

F

I

L

T

T

T

T

L

V

C

S

W

F

V

L

P

P

Q

Q

I

V

I

L

K

R

I

V

V

V

R

M

H

T

R

K

E

Q

T

T

A

R

S

D

I

I

S

S

L

R

V

N

M

M

N

Y

V

I

S

M

L
x
F

V

F

L

L

G

G

V

V

F

V

L

L

W
|
W

F

F

I

V

Y

Y

G

G

M

I

M

L

I

R

G

S

S

A

W

L

P

P

V

I

I

I

A

L

A

A

N

N

G

V

V

V

T

T

L

L

L

F

L

F

I

V

L

T

T

I

I

I

L

L

G

Y

L

Y

K

K

L

L

binding beta-D-glucopyranose: F41 (≠ L86), W48 (= W93)

P0DMV3 Sugar transporter SemiSWEET from Escherichia coli (strain UMEA 3162-1) (see paper)
46% identity, 41% coverage: 64:115/127 of query aligns to 21:72/89 of P0DMV3

query
sites
P0DMV3

P
|
P

Q
|
Q

I

S

I

I

K

K

I

T

V

I

R

R

H

T

R

R

E

N

T

T

A

E

S

G

I

I

S

S

L

V

V

V

M
|
M

N
x
Y

V

I

S

M

L

F

V

L

L

T

G

G

V

V

F

I

L

S

W
|
W

F

I

I

A

Y
|
Y

G

G

M

I

M

M

I
x
R

G

S

S

D

W

F

P

A

V

V

I

L

A

I

A

A

N
|
N

G

I

V

V

T

T

L

L

L

F

L

L

P21 (= P64) mutation to A: Strongly decreases sucrose transporter activity.
Q22 (= Q65) mutation to A: Slightly decreases sucrose transporter activity.
M39 (= M82) mutation to A: No effect on sucrose transporter activity.
Y40 (≠ N83) mutation to A: Decreases sucrose transporter activity.
W50 (= W93) mutation to A: Increases sucrose transporter activity.; mutation to F: No effect on sucrose transporter activity.
Y53 (= Y96) mutation to F: Increases sucrose transporter activity.
R57 (≠ I100) mutation to A: Increases sucrose transporter activity.
N66 (= N109) mutation to A: Impairs sucrose transporter activity.

Sites not aligning to the query:

19 F→A: Decreases sucrose transporter activity.

Query Sequence

>WP_106710428.1 NCBI__GCF_003010955.1:WP_106710428.1
MAQKARKIQNCRIQTLCHESAVFPRRICSYINCIPPVLKARSVPLHIELIGSLAAVITTL
CWVPQIIKIVRHRETASISLVMNVSLVLGVFLWFIYGMMIGSWPVIAANGVTLLLILTIL
GLKLRYG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory