SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_106711968.1 NCBI__GCF_003010955.1:WP_106711968.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53IZ1 Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; Asd; AsdP; EC 2.6.1.1; EC 4.1.1.12 from Pseudomonas sp. (see paper)
47% identity, 99% coverage: 5:534/536 of query aligns to 4:530/531 of Q53IZ1

query
sites
Q53IZ1

D

D

Y

Y

R

R

D

S

Y

L

E

A

K

N

M

L

S

S

P

P

F

F

E

E

I

L

K

K

D

D

A

E

L

L

I

I

K

K

L

V

A

A

K

-

R

-

S

-

S

S

Q

G

K

K

S

A

T

N

Q

R

V

L

L

M

L

L

N

N

A

A

G

G

R

R

G

G

N

N

P

P

N

N

W

F

I

L

A

A

T

T

K

T

P

P

R

R

E

R

G

A

F

F

L

R

F

L

G

G

Q

L

F

F

A

A

L

A

S

A

E

E

A

S

R

E

R

L

A

S

L

Y

D
x
S

N
x
Y

P
x
M

T

T

A

V

G

G

V

V

A

G

G

G

M

L

P

A

Q

K

Q

L

K

D

D

G

I

I

A

E

E

G

R

R

F

F

K

E

V

R

W

F

L

I

E

A

K

E

H

H

A

R

E

D

M

Q

E

E

G

G

A

V

E

K

F

F

L

L

S

G

K

K

M

S

V

L

D

S

H

Y

A

V

V

R

K

D

A

Q

F

L

G

G

F

L

D

D

A

P

D

A

A

A

F

F

V

L

F

H

E

E

L

M

T

V

D

D

S

G

I

I

I

L

G

G

D

C

N

N

Y

Y

P

P

V

V

P

P

D

P

R

R

M

M

L

L

V

T

H

V

A

S

E

E

Q

Q

I

I

A

V

H

R

R

Q

Y

Y

L

I

M

V

W

R

A

E

M

M

C

A

G

G

D

G

K

A

V

V

P

P

G

E

K

S

F

V

D

D

L

L

Y

F

A

A

V

V

E

E

G

G

T

T

A

A

M

M

C

A

Y

Y

I

I

F

F

K

E

S

S

L

L

I

R

A

I

N

S

R

G

I

L

L

L

K

K

K

A

G

G

D

D

T

K

I

V

A

A

L

I

G

G

T

M

P

P

I

V

F

F

T

T

P

P

Y

Y

I

I

E

E

I

I

A

P

E

E

L

L

E

A

D

Q

Y

Y

A

D

F

L

K

K

A

E

V

V

H

P

I

I

K

H

A

A

K

D

Q

P

E

D

N

N

R

G

F

W

Q

Q

Y

Y

S

S

D

D

E

A

E

E

I

L

K

D

K

K

L

L

E

K

D

D

P

P

K

D

I

V

K

K

A

I

F

F

V

C

V

V

N

N

P

P

G

S

N

N

P

P

T

P

S

S

M

V

A

K

I

M

D

D

A

Q

P

R

T

S

L

L

K

D

K

R

I

V

V

R

D

A

L

I

V

V

K

A

K

E

K

Q

R

R

P

P

D

D

L

L

L

I

L

L

T

T

D

D

V

V

Y

Y

G

G

T

T

F

F

V

A

P

D

G

E

F

F

R

Q

S

S

L

L

M

F

A

S

E

V

L

C

P

P

E

R

N

N

T

T

I

L

G

L

V

V

Y

Y

S

S

Y

F

S

S

K
|
K

Y

Y

F

F

G

G

C

A

T

T

G

G

W

W

R

R

L

L

G

G

V

V

I

I

A

A

I

A

H

H

E

K

K

D

N

N

I

V

L

F

D

D

S

H

T

A

I

L

A

S

K

Q

L

L

P

P

A

E

K

S

D

A

L

K

E

K

A

A

L

L

D

D

K

H

R

R

Y

Y

I

R

S

S

I

L

T

L

L

P

E

D

P

V

R

R

K

S

L

L

K

K

M

F

I

I

D

D

R

R

I

L

V

V

A

A

D

D

S

S

R

R

D

V

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

L

T

P

P

Q

Q

V

V

M

Q

M

M

T

V

M

L

F

F

S

S

M

L

S

F

E

A

L

L

M

M

D

D

T

E

E

A

K

D

L

A

Y

Y

Q

K

K

Q

A

A

C

L

M

K

D

Q

I

L

C

I

H

R

S

R

R

R

V

E

D

A

S

T

L

L

L

Y

E

R

G

E

L

L

P

G

L

M

D

P

V

P

Q

L

P

E

N

N

P

P

N

N

F

S

A

V

Y

N

Y

Y

G

T

T

L

I

I

D

D

F

L

E

Q

F

N

W

V

L

T

R

C

R

R

Y

L

V

Y

G

G

E

E

D

A

M

F

V

S

K

Q

W

W

L

A

K

V

K

Q

N

Q

V

S

H

S

P

T

L

G

D

D

L

M

V

L

F

F

K

R

L

V

A

A

E

D

D

E

H

T

A

G

I

I

V

V

L

L

N

P

G

G

G

R

G

G

F

F

D

G

A

S

P

D

N

R

W

P

S

S

V

G

R

R

V

A

S

S

F

L

A

A

N

N

L

L

T

N

D

E

D

Y

V

E

Y

Y

D

A

I

I

G

G

R

R

A

A

V

L

R

R

A

R

V

L

G

A

R

D

S

E

Y

L

V

Y

D

E

A

Q

F

Y

N

K

A

A

S

L

K

G

K

K

SYM 67:69 (≠ DNP 71:73) mutation to EEE: Loss of activity. Dissociation into dimers.
K314 (= K318) modified: N6-(pyridoxal phosphate)lysine

2zy2A Dodecameric l-aspartate beta-decarboxylase (see paper)
47% identity, 98% coverage: 13:536/536 of query aligns to 1:521/521 of 2zy2A

query
sites
2zy2A

S

S

P

P

F

F

E

E

I

L

K

K

D

D

A

E

L

L

I

I

K

K

L

V

A

A

K

-

R

-

S

-

S

S

Q

G

K

K

S

A

T

N

Q

R

V

L

L

M

L

L

N

N

A

A

G

G

R
|
R

G

G

N

N

P

P

N

N

W

F

I

L

A

A

T

T

K

T

P

P

R

R

E

R

G

A

F

F

L

R

F

L

G

G

Q

L

F

F

A

A

L

A

S

A

E

E

A

S

R

E

R

L

A

S

L

Y

D

S

N

Y

P

M

T

T

A

V

G

G

V

V

A

G

G

G

M

L

P

A

Q

K

Q

L

K

D

D

G

I

I

A

E

E

G

R

R

F

F

K

E

V

R

W

F

L

I

E

A

K

E

H

H

A

R

E

D

M

Q

E

E

G

G

A

V

E

K

F

F

L

L

S

G

K

K

M

S

V

L

D

S

H

Y

A

V

V

R

K

D

A

Q

F

L

G

G

F

L

D

D

A

P

D

A

A

A

F

F

V

L

F

H

E

E

L

M

T

V

D

D

S

G

I

I

I

L

G

G

D

C

N

N

Y

Y

P

P

V

V

P

P

D

P

R

R

M

M

L

L

V

T

H

V

A

S

E

E

Q

Q

I

I

A

V

H

R

R

Q

Y

Y

L

I

M

V

W

R

A

E

M

M

C

A

G

G

D

G

K

A

V

V

P

P

G

E

K

S

F

V

D

D

L

L

Y

F

A

A

V

V

E

E

G
|
G

T
|
T

A

A

M

M

C

A

Y

Y

I

I

F

F

K

E

S

S

L

L

I

R

A

I

N

S

R

G

I

L

L

L

K

K

K

A

G

G

D

D

T

K

I

V

A

A

L

I

G

G

T

M

P

P

I

V

F
|
F

T

T

P

P

Y

Y

I

I

E

E

I

I

A

P

E

E

L

L

E

A

D

Q

Y

Y

A

D

F

L

K

K

A

E

V

V

H

P

I

I

K

H

A

A

K

D

Q

P

E

D

N

N

R

G

F

W

Q

Q

Y

Y

S

S

D

D

E

A

E

E

I

L

K

D

K

K

L

L

E

K

D

D

P

P

K

D

I

V

K

K

A

I

F

F

V

C

V

V

N

N

P

P

G

S

N

N

P

P

T

P

S

S

M

V

A

K

I

M

D

D

A

Q

P

R

T

S

L

L

K

D

K

R

I

V

V

R

D

A

L

I

V

V

K

A

K

E

K

Q

R

R

P

P

D

D

L

L

L

I

L

L

T

T

D
|
D

D

D

V

V

Y
|
Y

G

G

T

T

F

F

V

A

P

D

G

E

F

F

R

Q

S

S

L

L

M

F

A

S

E

V

L

C

P

P

E

R

N

N

T

T

I

L

G

L

V

V

Y

Y

S
|
S

Y

F

S

S

K
|
K

Y

Y

F

F

G

G

C

A

T

T

G

G

W

W

R
|
R

L

L

G

G

V

V

I

I

A

A

I

A

H

H

E

K

K

D

N

N

I

V

L

F

D

D

S

H

T

A

I

L

A

S

K

Q

L

L

P

P

A

E

K

S

D

A

L

K

E

K

A

A

L

L

D

D

K

H

R

R

Y

Y

I

R

S

S

I

L

T

L

L

P

E

D

P

V

R

R

K

S

L

L

K

K

M

F

I

I

D

D

R

R

I

L

V

V

A

A

D

D

S

S

R

R

D

V

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

L

T

P

P

Q

Q

V

V

M

Q

M

M

T

V

M

L

F

F

S

S

M

L

S

F

E

A

L

L

M

M

D

D

T

E

E

A

K

D

L

A

Y

Y

Q

K

K

Q

A

A

C

L

M

K

D

Q

I

L

C

I

H

R

S

R

R

R

V

E

D

A

S

T

L

L

L

Y

E

R

G

E

L

L

P

G

L

M

D

P

V

P

Q

L

P

E

N

N

P

P

N

N

F

S

A

V

Y

N

Y

Y

G

T

T

L

I

I

D

D

F

L

E

Q

F

N

W

V

L

T

R

C

R

R

Y

L

V

Y

G

G

E

E

D

A

M

F

V

S

K

Q

W

W

L

A

K

V

K

Q

N

Q

V

S

H

S

P

T

L

G

D

D

L

M

V

L

F

F

K

R

L

V

A

A

E

D

D

E

H

T

A

G

I

I

V

V

L

L

N

P

G

G

G

R

G

G

F

F

D

G

A

S

P

D

N

R

W

P

S

S

V

G

R

R

V

A

S

S

F

L

A

A

N

N

L

L

T

N

D

E

D

Y

V

E

Y

Y

D

A

I

I

G

G

R

R

A

A

V

L

R

R

A

R

V

L

G

A

R

D

S

E

Y

L

V

Y

D

E

A

Q

F

Y

N

K

A

A

S

L

K

G

K

K

G

E

K

K

binding pyridoxal-5'-phosphate: R26 (= R41), G161 (= G176), G162 (= G177), T163 (= T178), F192 (= F207), D274 (= D289), Y277 (= Y292), S300 (= S315), K303 (= K318), R311 (= R326)

Q93QX0 Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; ASD; AsdA; EC 2.6.1.1; EC 4.1.1.12 from Comamonas testosteroni (Pseudomonas testosteroni) (see paper)
46% identity, 96% coverage: 5:519/536 of query aligns to 4:516/533 of Q93QX0

query
sites
Q93QX0

D

D

Y

Y

R

Q

D

S

Y

L

E

A

K

K

M

L

S

S

P

P

F

F

E

E

I

L

K

K

D

D

A

E

L

L

I

I

K

K

L

I

A

A

K

-

R

-

S

-

S

S

Q

S

K

D

S

G

T

N

Q

R

V

L

L

M

L

L

N

N

A

A

G

G

R

R

G

G

N

N

P

P

N

N

W

F

I

L

A

A

T

T

K

T

P

P

R

R

E

R

G

A

F

F

L

R

F

L

G

G

Q

L

F

F

A

A

L

A

S

A

E

E

A

S

R

E

R

L

A

S

L

Y

D

S

N

Y

-

M

P

T

T

T

A

V

G

G

V

V

A

G

G

G

M

L

P

A

Q

K

Q

I

K

D

D

G

I

I

A

E

E

G

R

R

F

F

K

E

V

R

W

Y

L

I

E

A

K

E

H

N

A

R

E

D

M

Q

E

E

G

G

A

V

E

R

F

F

L

L

S

G

K

K

M

S

V

L

D

S

H

Y

A

V

V

R

K

D

A

Q

F

L

G

G

F

L

D

D

A

P

D

A

A

A

F

F

V

L

F

H

E

E

L

M

T

V

D

D

S

G

I

I

I

L

G

G

D

C

N

N

Y
|
Y

P

P

V

V

P

P

D

P

R

R

M

M

L

L

V

N

H

I

A

S

E

E

Q

K

I

I

A

V

H

R

R

Q

Y

Y

L

I

M

I

W

R

A

E

M

M

C

G

G

A

D

D

K

A

V

I

P

P

P

S

G

E

K

S

F

V

D

N

L

L

Y

F

A

A

V

V

E

E

G

G

T

T

A

A

M

M

C

A

Y

Y

I

I

F

F

K

E

S

S

L

L

I

K

A

L

N

N

R

G

I

L

L

L

K

K

K

A

G

G

D

D

T

K

I

V

A

A

L

I

G

G

T

M

P

P

I

V

F

F

T

T

P

P

Y

Y

I

I

E

E

I

I

A

P

E

E

L

L

E

A

D

Q

Y

Y

A

A

F

L

K

E

A

E

V

V

H

A

I

I

K

N

A

A

K

D

Q

P

E

S

N

L

R

N

F

W

Q

Q

Y

Y

S

P

D

D

E

S

E

E

I

L

K

D

K

K

L

L

E

K

D

D

P

P

K

A

I

I

K

K

A

I

F

F

V

C

V

V

N

N

P

P

G

S

N

N

P

P

T

P

S

S

M

V

A

K

I

M

D

D

A

Q

P

R

T

S

L

L

K

E

K

R

I

V

V

R

D

N

L

I

V

V

K

A

K

E

K

H

R

R

P

P

D

D

L

L

L

M

L

I

L

L

T

T

D

D

V

V

Y

Y

G

G

T

T

F

F

V

A

P

D

G

D

F

F

R

Q

S

S

L

L

M

F

A

A

E

I

L

C

P

P

E

E

N

N

T

T

I

L

G

L

V

V

Y

Y

S

S

Y

F

S

S

K
|
K

Y

Y

F

F

G

G

C

A

T

T

G

G

W

W

R

R

L

L

G

G

V

V

I

V

A

A

I

A

H

H

E

Q

K

Q

N

N

I

V

L

F

D

D

S

L

T

A

I

L

A

D

K

K

L

L

P

Q

A

E

K

S

D

E

L

K

E

V

A

A

L

L

D

D

K

H

R

R

Y

Y

I

R

S

S

I

L

T

L

L

P

E

D

P

V

R

R

K

S

L

L

K

K

M

F

I

I

D

D

R

R

I

L

V

V

A

A

D

D

S

S

R

R

D

A

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

L

T

P

P

Q

Q

V

V

M

Q

M

M

T

A

M

L

F

F

S

S

M

L

S

F

E

A

L

L

M

M

D

D

T

E

E

A

K

D

L

E

Y

Y

Q

K

K

H

A

T

C

L

M

K

D

Q

I

L

C

I

H

R

S

R

R

R

V

E

D

T

S

T

L

L

L

Y

E

R

G

E

L

L

P

G

L

M

D

P

V

P

Q

L

P

R

N

D

P

E

N

N

F

A

A

V

Y

D

Y

Y

G

T

T

L

I

I

D

D

F

L

E

Q

F

D

W

V

L

T

R

A

R

K

Y

L

V

Y

G

G

E

E

D

A

M

F

V

S

K

E

W

W

L

A

K

V

K

K

N

Q

V

S

H

S

P

T

L

G

D

D

L

M

V

L

F

F

K

R

L

I

A

A

E

D

D

E

H

T

A

G

I

I

V

V

L

L

N

P

G

G

G
x
R

G

G

F

F

D

G

A

S

P

N

N

R

W

P

S

S

V

G

R

R

V

A

S

S

F

L

A

A

N

N

L

L

T

N

D

E

D

Y

V

E

Y

Y

D

A

I

I

G

G

R

R

A

A

V

L

R

R

Y134 (= Y137) mutation to F: Slightly reduced activity.
K315 (= K318) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Slightly reduced activity.
R487 (≠ G490) mutation to A: Loss of activity.

2zy4F Dodecameric l-aspartate beta-decarboxylase (see paper)
46% identity, 95% coverage: 11:519/536 of query aligns to 5:511/535 of 2zy4F

query
sites
2zy4F

K

K

M

L

S

S

P

P

F

F

E

E

I

L

K

K

D

D

A

E

L

L

I

I

K

K

L

I

A

A

K

-

R

-

S

-

S

S

Q

S

K

D

S

G

T

N

Q

R

V

L

L

M

L

L

N

N

A

A

G

G

R
|
R

G

G

N

N

P

P

N

N

W

F

I

L

A

A

T

T

K

T

P

P

R

R

E

R

G

A

F

F

L

R

F

L

G

G

Q

L

F

F

A

A

L

A

S

A

E

E

A

S

R

E

R

L

A

S

L

Y

D

S

N

Y

-

M

P

T

T

T

A

V

G

G

V

V

A

G

G

G

M

L

P

A

Q

K

Q

I

K

D

D

G

I

I

A

E

E

G

R

R

F

F

K

E

V

R

W

Y

L

I

E

A

K

E

H

N

A

R

E

D

M

Q

E

E

G

G

A

V

E

R

F

F

L

L

S

G

K

K

M

S

V

L

D

S

H

Y

A

V

V

R

K

D

A

Q

F

L

G

G

F

L

D

D

A

P

D

A

A

A

F

F

V

L

F

H

E

E

L

M

T

V

D

D

S

G

I

I

I

L

G

G

D

C

N

N

Y

Y

P

P

V

V

P

P

D

P

R

R

M

M

L

L

V

N

H

I

A

S

E

E

Q

K

I

I

A

V

H

R

R

Q

Y

Y

L

I

M

I

W

R

A

E

M

M

C

G

G

A

D

D

K

A

V

I

P

P

P

S

G

E

K

S

F

V

D

N

L

L

Y

F

A

A

V

V

E

E

G
|
G

T
|
T

A

A

M

M

C

A

Y

Y

I

I

F

F

K

E

S

S

L

L

I

K

A

L

N

N

R

G

I

L

L

L

K

K

K

A

G

G

D

D

T

K

I

V

A

A

L

I

G

G

T

M

P

P

I

V

F
|
F

T

T

P

P

Y

Y

I

I

E

E

I

I

A

P

E

E

L

L

E

A

D

Q

Y

Y

A

A

F

L

K

E

A

E

V

V

H

A

I

I

K

N

A

A

K

D

Q

P

E

S

N

L

R

N

F

W

Q

Q

Y

Y

S

P

D

D

E

S

E

E

I

L

K

D

K

K

L

L

E

K

D

D

P

P

K

A

I

I

K

K

A

I

F

F

V

C

V

V

N

N

P

P

G

S

N

N

P

P

T

P

S

S

M

V

A

K

I

M

D

D

A

Q

P

R

T

S

L

L

K

E

K

R

I

V

V

R

D

N

L

I

V

V

K

A

K

E

K

H

R

R

P

P

D

D

L

L

L

M

L

I

L

L

T

T

D
|
D

D

D

V
|
V

Y
|
Y

G

G

T

T

F

F

V

A

P

D

G

D

F

F

R

Q

S

S

L

L

M

F

A

A

E

I

L

C

P

P

E

E

N

N

T

T

I

L

G

L

V

V

Y

Y

S
|
S

Y

F

S
|
S

K
|
K

Y

Y

F

F

G

G

C

A

T

T

G

G

W

W

R
|
R

L

L

G

G

V

V

I

V

A

A

I

A

H

H

E

Q

K

Q

N

N

I

V

L

F

D

D

S

L

T

A

I

L

A

D

K

K

L

L

P

Q

A

E

K

S

D

E

L

K

E

V

A

A

L

L

D

D

K

H

R

R

Y

Y

I

R

S

S

I

L

T

L

L

P

E

D

P

V

R

R

K

S

L

L

K

K

M

F

I

I

D

D

R

R

I

L

V

V

A

A

D

D

S

S

R

R

D

A

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

L

T

P

P

Q

Q

V

V

M

Q

M

M

T

A

M

L

F

F

S

S

M

L

S

F

E

A

L

L

M

M

D

D

T

E

E

A

K

D

L

E

Y

Y

Q

K

K

H

A

T

C

L

M

K

D

Q

I

L

C

I

H

R

S

R

R

R

V

E

D

T

S

T

L

L

L

Y

E

R

G

E

L

L

P

G

L

M

D

P

V

P

Q

L

P

R

N

D

P

E

N

N

F

A

A

V

Y

D

Y

Y

G

T

T

L

I

I

D

D

F

L

E

Q

F

D

W

V

L

T

R

A

R

K

Y

L

V

Y

G

G

E

E

D

A

M

F

V

S

K

E

W

W

L

A

K

V

K

K

N

Q

V

S

H

S

P

T

L

G

D

D

L

M

V

L

F

F

K

R

L

I

A

A

E

D

D

E

H

T

A

G

I

I

V

V

L

L

N

P

G

G

G

R

G

G

F

F

D

G

A

S

P

N

N

R

W

P

S

S

V

G

R

R

V

A

S

S

F

L

A

A

N

N

L

L

T

N

D

E

D

Y

V

E

Y

Y

D

A

I

I

G

G

R

R

A

A

V

L

R

R

binding pyridoxal-5'-phosphate: R32 (= R41), G168 (= G176), G169 (= G177), T170 (= T178), F199 (= F207), D281 (= D289), V283 (= V291), Y284 (= Y292), S307 (= S315), S309 (= S317), K310 (= K318), R318 (= R326)

1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
33% identity, 26% coverage: 194:331/536 of query aligns to 107:245/388 of 1gdeA

query
sites
1gdeA

L

L

K

K

K

D

G

G

D

E

T

E

I

V

A

L

L

I

G

P

T

T

P

P

I

A

F
|
F

T

V

P

S

Y

Y

I

A

E

P

I

A

A

V

E

I

L

L

E

A

D

-

Y

-

A

G

F

G

K

K

A

P

V

V

H

E

I

V

K

P

A

T

K

Y

Q

E

E

E

N

D

R

E

F

F

Q

R

Y

L

S

N

D

V

E

D

E

E

I

L

K

K

L

Y

E

V

D

T

P

D

K

K

I

T

K

R

A

A

F

L

F

I

V

I

V
x
N

N

S

P

P

G

C

N
|
N

P

P

T

T

S

G

M

A

A

V

I

L

D

T

A

K

P

K

T

D

L

L

K

E

I

I

V

A

D

D

L

F

V

V

K

V

K

E

K

H

R

-

P

-

D

D

L

L

L

I

L

V

L

I

T

S

D
|
D

D

E

V

V

Y
|
Y

G

E

T

H

F

F

V

I

-

Y

-

D

P

D

G

A

F

R

R

H

S

Y

L

S

M

I

A

A

E

S

L

L

P

D

-

G

-

M

-

F

E

E

N

R

T

T

I

I

G

T

V

V

Y

N

S

G

Y

F

S
|
S

K
|
K

Y

T

F

F

G

A

C

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

F

I

V

A

A

active site: K232 (= K318)
binding glutamic acid: F120 (= F207), N170 (= N259)
binding pyridoxal-5'-phosphate: F120 (= F207), N166 (≠ V255), D198 (= D289), Y201 (= Y292), S231 (= S317), K232 (= K318), R240 (= R326)

Sites not aligning to the query:

1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
33% identity, 26% coverage: 194:331/536 of query aligns to 107:245/388 of 1gd9A

query
sites
1gd9A

L

L

K

K

K

D

G

G

D

E

T

E

I

V

A

L

L

I

G

P

T

T

P

P

I

A

F
|
F

T

V

P

S

Y

Y

I

A

E

P

I

A

A

V

E

I

L

L

E

A

D

-

Y

-

A

G

F

G

K

K

A

P

V

V

H

E

I

V

K

P

A

T

K

Y

Q

E

E

E

N

D

R

E

F

F

Q

R

Y

L

S

N

D

V

E

D

E

E

I

L

K

K

L

Y

E

V

D

T

P

D

K

K

I

T

K

R

A

A

F

L

F

I

V

I

V

N

N

S

P

P

G

C

N
|
N

P

P

T

T

S

G

M

A

A

V

I

L

D

T

A

K

P

K

T

D

L

L

K

E

I

I

V

A

D

D

L

F

V

V

K

V

K

E

K

H

R

-

P

-

D

D

L

L

L

I

L

V

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G

E

T

H

F

F

V

I

-

Y

-

D

P

D

G

A

F

R

R

H

S

Y

L

S

M

I

A

A

E

S

L

L

P

D

-

G

-

M

-

F

E

E

N

R

T

T

I

I

G

T

V

V

Y

N

S

G

Y

F

S
|
S

K
|
K

Y

T

F

F

G

A

C

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

F

I

V

A

A

active site: K232 (= K318)
binding pyridoxal-5'-phosphate: F120 (= F207), N170 (= N259), D198 (= D289), V200 (= V291), Y201 (= Y292), S231 (= S317), K232 (= K318), R240 (= R326)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 94, 95, 96

Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
30% identity, 29% coverage: 177:331/536 of query aligns to 97:252/400 of Q02635

query
sites
Q02635

G

G

T

T

A

G

M

K

C

Q

Y

I

I

L

F

F

K

N

S

A

L

F

I

M

A

A

N

T

R

-

I

-

L

L

K

N

K

P

G

G

D

D

T

E

I

V

A

V

L

I

G

P

T

A

P

P

I

Y

F

W

T

V

P

S

Y

Y

I

P

E

E

I

M

A

V

E

A

L

L

E

-

D

-

Y

C

A

G

F

G

K

T

A

P

V

V

H

F

I

V

K

P

A

T

K

R

Q

Q

E

E

N

N

R

N

F

F

Q

K

Y

L

S

K

D

A

E

E

E

D

I

L

K

D

K

R

L

A

E

I

D

T

P

P

K

K

I

T

K

K

A

W

F

F

F

V

V

F

V

N

N

S

P

P

G

S

N

N

P

P

T

S

S

G

M

A

A

A

I

Y

D

S

A

H

P

E

T

E

L

L

K

K

K

A

I

L

V

T

D

D

L

V

V

L

K

-

K

M

K

K

R

H

P

P

D

H

L

V

L

W

L

V

L

L

T

T

D

D

V

M

Y

Y

G

E

T

H

F

L

V

T

P

Y

G

G

-

D

F

F

R

R

-

F

-

A

-

T

-

P

-

V

S

E

L

V

M

E

A

P

E

G

L

L

P

Y

E

E

N

R

T

T

I

L

G

T

V

M

Y

N

S

G

Y

V

S

S

K

K

Y

A

F

Y

G

A

C

M

T

T

G

G

W

W

R

R

L

I

G

G

V

Y

I

A

A

A

Sites not aligning to the query:

12 Important for prephenate aminotransferase activity; K→G: 7-fold increase in Km for prephenate. Weakly affects Km for oxaloacetate.

6f77A Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
30% identity, 29% coverage: 177:331/536 of query aligns to 96:251/399 of 6f77A

query
sites
6f77A

G

G

T

T

A
x
G

M
x
K

C

Q

Y

I

I

L

F

F

K

N

S

A

L

F

I

M

A

A

N

T

R

-

I

-

L

L

K

N

K

P

G

G

D

D

T

E

I

V

A

V

L

I

G

P

T

A

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

P

E

E

I

M

A

V

E

A

L

L

E

-

D

-

Y

C

A

G

F

G

K

T

A

P

V

V

H

F

I

V

K

P

A

T

K

R

Q

Q

E

E

N

N

R

N

F

F

Q

K

Y

L

S

K

D

A

E

E

E

D

I

L

K

D

K

R

L

A

E

I

D

T

P

P

K

K

I

T

K

K

A

W

F

F

F

V

V

F

V
x
N

N

S

P

P

G

S

N
|
N

P

P

T

S

S

G

M

A

A

A

I

Y

D

S

A

H

P

E

T

E

L

L

K

K

K

A

I

L

V

T

D

D

L

V

V

L

K

-

K

M

K

K

R

H

P

P

D

H

L

V

L

W

L

V

L

L

T

T

D
|
D

D

D

V

M

Y
|
Y

G

E

T

H

F

L

V

T

P

Y

G

G

-

D

F

F

R

R

-

F

-

A

-

T

-

P

-

V

S

E

L

V

M

E

A

P

E

G

L

L

P

Y

E

E

N

R

T

T

I

L

G

T

V

M

Y

N

S

G

Y

V

S
|
S

K
|
K

Y

A

F

Y

G

A

C

M

T

T

G

G

W

W

R
|
R

L

I

G

G

V

Y

I

A

A

A

binding pyridoxal-5'-phosphate: G98 (≠ A179), G99 (≠ A180), K100 (≠ M181), W124 (≠ F207), N170 (≠ V255), N174 (= N259), D203 (= D289), Y206 (= Y292), S237 (= S317), K238 (= K318), R246 (= R326)

5wmiA Arabidopsis thaliana prephenate aminotransferase mutant- t84v (see paper)
31% identity, 26% coverage: 193:331/536 of query aligns to 109:249/402 of 5wmiA

query
sites
5wmiA

I

V

L

C

K

S

K

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

T

A

P

P

I

Y

F

W

T

V

P

S

Y

Y

I

T

E

E

I

Q

A

A

E

R

L

L

E

A

D

D

Y

A

A

T

F

-

K

-

A

P

V

V

H

V

I

I

K

P

A

T

K

K

Q

I

E

S

N

N

R

N

F

F

Q

L

Y

L

S

D

D

P

E

K

E

D

I

L

K

E

K

S

L

K

E

L

D

T

P

E

K

K

I

S

K

R

A

L

F

L

F

I

V

L

V

C

N

S

P

P

G

S

N
|
N

P

P

T

T

S

G

M

S

A

V

I

Y

D

P

A

K

P

S

T

L

L

L

K

E

I

I

V

A

D

R

L

I

V

I

K

A

K

K

K

-

R

H

P

P

D

R

L

L

L

V

L

L

T

S

D

D

D

E

V

I

Y

Y

G

E

-

H

-

I

T

I

F

Y

V

A

P

P

G

A

F

T

R

H

S

T

L

S

M

F

A

A

E

S

L

L

P

P

-

D

-

M

-

Y

E

E

N

R

T

T

I

L

G

T

V

V

Y

N

S

G

Y

F

S

S

K
|
K

Y

A

F

F

G

A

C

M

T

T

G

G

W

W

R

R

L

L

G

G

V

Y

I

L

A

A

binding 2-oxoglutaric acid: N173 (= N259), K236 (= K318)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 15, 36, 375

5wmhA Arabidopsis thaliana prephenate aminotransferase (see paper)
31% identity, 26% coverage: 193:331/536 of query aligns to 109:249/399 of 5wmhA

query
sites
5wmhA

I

V

L

C

K

S

K

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

T

A

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

T

E

E

I

Q

A

A

E

R

L

L

E

A

D

D

Y

A

A

T

F

-

K

-

A

P

V

V

H

V

I

I

K

P

A

T

K

K

Q

I

E

S

N

N

R

N

F

F

Q

L

Y

L

S

D

D

P

E

K

E

D

I

L

K

E

K

S

L

K

E

L

D

T

P

E

K

K

I

S

K

R

A

L

F

L

F

I

V

L

V

C

N

S

P

P

G

S

N
|
N

P

P

T

T

S

G

M

S

A

V

I

Y

D

P

A

K

P

S

T

L

L

L

K

E

I

I

V

A

D

R

L

I

V

I

K

A

K

K

K

-

R

H

P

P

D

R

L

L

L

V

L

L

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

E

-

H

-

I

T

I

F

Y

V

A

P

P

G

A

F

T

R

H

S

T

L

S

M

F

A

A

E

S

L

L

P

P

-

D

-

M

-

Y

E

E

N

R

T

T

I

L

G

T

V

V

Y

N

S

G

Y

F

S
|
S

K
|
K

Y

A

F

F

G

A

C

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

Y

I

L

A

A

binding pyridoxal-5'-phosphate: W123 (≠ F207), N173 (= N259), D202 (= D289), I204 (≠ V291), Y205 (= Y292), S235 (= S317), K236 (= K318), R244 (= R326)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 97, 98, 99

8wkjA The crystal structure of aspartate aminotransferases lpg0070 from legionella pneumophila (see paper)
29% identity, 26% coverage: 194:331/536 of query aligns to 112:251/391 of 8wkjA

query
sites
8wkjA

L

L

K

N

K

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

T

A

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

P

E

D

I

M

A

V

E

L

L

L

E

A

D

D

Y

G

A

V

F

-

K

-

A

P

V

V

H

I

I

I

K

E

A

T

K

T

Q

P

E

A

N

Q

R

R

F

Y

Q

K

Y

I

S

N

D

A

E

Q

I

L

K

E

K

Q

L

A

E

I

D

T

P

P

K

K

I

T

K

R

A

M

F

I

F

F

V

L

V
x
N

N

S

P

P

G

S

N
|
N

P

P

T

S

S

G

M

I

A

A

I

Y

D

T

A

Q

P

N

T

E

L

L

K

K

K

E

I

L

V

G

D

D

L

V

V

L

K

-

K

K

R

H

P

P

D

Q

L

I

L

L

L

I

L

A

T

T

D
|
D

D

D

V

M

Y
|
Y

G

E

T

H

F

I

V

I

-

W

-

S

P

Q

G

P

F

F

R

T

S

N

L

I

M

L

A

N

E

A

L

C

P

P

E

E

-

L

-

Y

-

D

N

R

T

T

I

I

G

V

V

L

Y

N

S

G

Y

V

S
|
S

K
|
K

Y

A

F

Y

G

A

C

M

T

T

G

G

W

W

R
|
R

L

I

G

G

V

Y

I

A

A

A

binding pyridoxal-5'-phosphate: W125 (≠ F207), N171 (≠ V255), N175 (= N259), D204 (= D289), Y207 (= Y292), S237 (= S317), K238 (= K318), R246 (= R326)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 99, 100, 101

5yhvB Crystal structure of an aminotransferase from mycobacterium tuberculosis
30% identity, 25% coverage: 197:328/536 of query aligns to 114:243/387 of 5yhvB

query
sites
5yhvB

G

G

D

D

T

R

I

V

A

A

L

M

G

A

T

S

P

P

I

G

F
x
Y

T

P

P

C

Y

Y

I

R

E

N

I

I

A

-

E

-

L

L

E

S

D

A

Y

L

A

G

F

C

K

E

A

V

V

V

H

E

I

I

K

P

A

C

K

G

Q

P

E

Q

N

T

R

R

F

F

Q

Q

Y

P

S

T

D

A

E

Q

E

M

I

L

K

A

K

E

L

I

E

-

D

D

P

P

K

P

I

L

K

R

A

G

F

V

V

V

V

A

N

S

P

P

G

A

N
|
N

P

P

T

T

S

G

M

T

A

V

I

I

D

P

A

P

P

E

T

E

L

L

K

A

I

I

V

A

D

S

L

W

V

C

K

D

K

A

K

S

R

-

P

-

D

D

L

V

L

R

L

L

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G
x
H

T

G

F

L

V

V

-

Y

-

Q

-

G

P

A

G

P

F

Q

R
x
T

S

S

L

C

M

A

A

W

E

Q

L

T

P

S

E

R

N

N

T

A

I

V

G

V

V

V

Y
x
N

S
|
S

Y

F

S
|
S

K
|
K

Y

Y

F

Y

G

A

C

M

T

T

G

G

W

W

R
|
R

L

L

G

G

binding 2-oxoglutaric acid: H205 (≠ G293), T215 (≠ R300), N229 (≠ Y314)
binding pyridoxal-5'-phosphate: Y124 (≠ F207), N173 (= N259), D201 (= D289), V203 (= V291), Y204 (= Y292), S230 (= S315), S232 (= S317), K233 (= K318), R241 (= R326)

Sites not aligning to the query:

5yhvA Crystal structure of an aminotransferase from mycobacterium tuberculosis
30% identity, 25% coverage: 197:328/536 of query aligns to 121:250/394 of 5yhvA

query
sites
5yhvA

G

G

D

D

T

R

I

V

A

A

L

M

G

A

T

S

P

P

I

G

F
x
Y

T

P

P

C

Y

Y

I

R

E

N

I

I

A

-

E

-

L

L

E

S

D

A

Y

L

A

G

F

C

K

E

A

V

V

V

H

E

I

I

K

P

A

C

K

G

Q

P

E

Q

N

T

R

R

F

F

Q

Q

Y

P

S

T

D

A

E

Q

E

M

I

L

K

A

K

E

L

I

E

-

D

D

P

P

K

P

I

L

K

R

A

G

F

V

V

V

V

A

N

S

P

P

G

A

N
|
N

P

P

T

T

S

G

M

T

A

V

I

I

D

P

A

P

P

E

T

E

L

L

K

A

I

I

V

A

D

S

L

W

V

C

K

D

K

A

K

S

R

-

P

-

D

D

L

V

L

R

L

L

L

I

T

S

D

D

D

E

V

V

Y
|
Y

G
x
H

T

G

F

L

V

V

-

Y

-

Q

-

G

P

A

G

P

F

Q

R

T

S

S

L

C

M

A

A

W

E

Q

L

T

P

S

E

R

N

N

T

A

I

V

G

V

V

V

Y

N

S
|
S

Y

F

S
|
S

K
|
K

Y

Y

F

Y

G

A

C

M

T

T

G

G

W

W

R
|
R

L

L

G

G

binding glutamic acid: H212 (≠ G293)
binding pyridoxal-5'-phosphate: Y131 (≠ F207), N180 (= N259), Y211 (= Y292), S237 (= S315), S239 (= S317), K240 (= K318), R248 (= R326)

Sites not aligning to the query:

P96847 Valine--pyruvate aminotransferase; Alanine--valine transaminase; EC 2.6.1.66 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
30% identity, 25% coverage: 197:328/536 of query aligns to 115:244/388 of P96847

query
sites
P96847

G

G

D

D

T

R

I

V

A

A

L

M

G

A

T

S

P

P

I

G

F

Y

T

P

P

C

Y

Y

I

R

E

N

I

I

A

-

E

-

L

L

E

S

D

A

Y

L

A

G

F

C

K

E

A

V

V

V

H

E

I

I

K

P

A

C

K

G

Q

P

E

Q

N

T

R

R

F

F

Q

Q

Y

P

S

T

D

A

E

Q

E

M

I

L

K

A

K

E

L

I

E

-

D

D

P

P

K

P

I

L

K

R

A

G

F

V

V

V

V

A

N

S

P

P

G

A

N

N

P

P

T

T

S

G

M

T

A

V

I

I

D

P

A

P

P

E

T

E

L

L

K

A

I

I

V

A

D

S

L

W

V

C

K

D

K

A

K

S

R

-

P

-

D

D

L

V

L

R

L

L

L

I

T

S

D

D

D

E

V

V

Y

Y

G

H

T

G

F

L

V

V

-

Y

-

Q

-

G

P

A

G

P

F

Q

R

T

S

S

L

C

M

A

A

W

E

Q

L

T

P

S

E

R

N

N

T

A

I

V

G

V

V

V

Y

N

S

S

Y

F

S

S

K
|
K

Y

Y

F

Y

G

A

C

M

T

T

G

G

W

W

R

R

L

L

G

G

K234 (= K318) modified: N6-(pyridoxal phosphate)lysine

5wmlA Arabidopsis thaliana prephenate aminotransferase mutant- k306a (see paper)
30% identity, 26% coverage: 193:331/536 of query aligns to 110:250/404 of 5wmlA

query
sites
5wmlA

I

V

L

C

K

S

K

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

T

A

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

T

E

E

I

Q

A

A

E

R

L

L

E

A

D

D

Y

A

A

T

F

-

K

-

A

P

V

V

H

V

I

I

K

P

A

T

K

K

Q

I

E

S

N

N

R

N

F

F

Q

L

Y

L

S

D

D

P

E

K

E

D

I

L

K

E

K

S

L

K

E

L

D

T

P

E

K

K

I

S

K

R

A

L

F

L

F

I

V

L

V

C

N

S

P

P

G

S

N
|
N

P

P

T

T

S

G

M

S

A

V

I

Y

D

P

A

K

P

S

T

L

L

L

K

E

I

I

V

A

D

R

L

I

V

I

K

A

K

K

K

-

R

H

P

P

D

R

L

L

L

V

L

L

T

S

D
|
D

D

E

V

I

Y
|
Y

G

E

-

H

-

I

T

I

F

Y

V

A

P

P

G

A

F

T

R

H

S

T

L

S

M

F

A

A

E

S

L

L

P

P

-

D

-

M

-

Y

E

E

N

R

T

T

I

L

G

T

V

V

Y

N

S

G

Y

F

S
|
S

K

A

Y

A

F

F

G

A

C

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

Y

I

L

A

A

binding glutamic acid: W124 (≠ F207)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: W124 (≠ F207), N174 (= N259), D203 (= D289), Y206 (= Y292), S236 (= S317), R245 (= R326)

Sites not aligning to the query:

binding glutamic acid: 15, 16, 37, 38, 100, 326, 376
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: 98, 99, 100, 103

P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
27% identity, 30% coverage: 169:328/536 of query aligns to 99:259/410 of P58350

query
sites
P58350

F

Y

D

E

L

L

Y

D

A

E

V

I

E

T

G

V

G

A

T

T

A

G

A

A

M

K

C

Q

Y

I

I

L

F

F

K

N

S

A

L

M

I

M

A

A

N

S

R

-

I

-

L

L

K

D

K

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

T

T

P

P

I

Y

F

W

T

T

P

S

Y

Y

I

S

E

D

I

I

A

V

E

H

L

I

E

C

D

E

Y

-

A

-

F

G

K

K

A

P

V

V

H

L

I

I

K

A

A

C

K

D

Q

A

E

S

N

S

R

G

F

F

Q

R

Y

L

S

T

D

A

E

E

E

K

I

L

K

E

K

A

L

A

E

I

D

T

P

P

K

R

I

T

K

R

A

W

F

V

F

L

V

L

V

N

N

S

P

P

G

S

N

N

P

P

T

S

S

G

M

A

A

A

I

Y

D

S

A

A

P

A

T

D

L

Y

K

R

K

P

I

L

V

L

D

E

L

V

V

L

K

L

K

R

K

-

R

H

P

P

D

H

L

V

L

W

L

L

T

V

D

D

V

M

Y

Y

G

E

T

H

F

I

V

V

-

Y

P

D

G

G

F

F

R

R

-

F

-

V

-

T

-

P

-

A

S

Q

L

L

M

E

A

P

E

G

L

L

P

K

E

N

N

R

T

T

I

L

G

T

V

V

Y

N

S

G

Y

V

S

S

K
|
K

Y

A

F

Y

G

A

C

M

T

T

G

G

W

W

R

R

L

I

G

G

K249 (= K318) modified: N6-(pyridoxal phosphate)lysine

6f35A Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
27% identity, 30% coverage: 169:328/536 of query aligns to 89:249/400 of 6f35A

query
sites
6f35A

F

Y

D

E

L

L

Y

D

A

E

V

I

E

T

G

V

G

A

T

T

A
x
G

A
|
A

M
x
K

C

Q

Y

I

I

L

F

F

K

N

S

A

L

M

I

M

A

A

N

S

R

-

I

-

L

L

K

D

K

P

G

G

D

D

T

E

I

V

A

I

L

I

G

P

T

T

P

P

I

Y

F

W

T

T

P

S

Y

Y

I

S

E

D

I

I

A

V

E

H

L

I

E

C

D

E

Y

-

A

-

F

G

K

K

A

P

V

V

H

L

I

I

K

A

A

C

K

D

Q

A

E

S

N

S

R

G

F

F

Q

R

Y

L

S

T

D

A

E

E

E

K

I

L

K

E

K

A

L

A

E

I

D

T

P

P

K

R

I

T

K

R

A

W

F

V

F

L

V

L

V

N

N

S

P

P

G

S

N

N

P

P

T

S

S

G

M

A

A

A

I

Y

D

S

A

A

P

A

T

D

L

Y

K

R

K

P

I

L

V

L

D

E

L

V

V

L

K

L

K

R

K

-

R

H

P

P

D

H

L

V

L

W

L

L

T

V

D
|
D

D

D

V
x
M

Y
|
Y

G

E

T

H

F

I

V

V

-

Y

P

D

G

G

F

F

R

R

-

F

-

V

-

T

-

P

-

A

S

Q

L

L

M

E

A

P

E

G

L

L

P

K

E

N

N

R

T

T

I

L

G

T

V

V

Y

N

S

G

Y

V

S
|
S

K
|
K

Y

A

F

Y

G

A

C

M

T

T

G

G

W

W

R
|
R

L

I

G

G

binding pyridoxal-5'-phosphate: G99 (≠ A179), A100 (= A180), K101 (≠ M181), D204 (= D289), M206 (≠ V291), Y207 (= Y292), S238 (= S317), K239 (= K318), R247 (= R326)

1xi9C Alanine aminotransferase from pyrococcus furiosus pfu-1397077-001
30% identity, 28% coverage: 178:328/536 of query aligns to 97:245/393 of 1xi9C

query
sites
1xi9C

T

T

A

A

A
|
A

M
x
V

C
x
T

Y

E

I

A

F

L

K

Q

S

-

L

L

I

I

A

F

N

G

R

A

I

L

L

L

K

D

K

P

G

G

D

D

T

E

I

I

A

L

L

V

G

P

T

G

P

P

I

S

F
x
Y

T

P

P

P

Y

Y

I

T

E

G

I

L

A

V

E

K

L

F

E

-

D

-

Y

Y

A

G

F

G

K

K

A

P

V

V

H

E

I

Y

K

R

A

T

K

I

Q

E

E

E

N

E

R

D

F

W

Q

Q

Y

P

S

D

D

I

E

D

I

I

K

R

K

K

L

K

E

I

D

T

P

D

K

R

I

T

K

K

A

A

F

I

F

A

V

V

V

I

N

N

P

P

G

N

N
|
N

P

P

T

T

S

G

M

A

A

L

I

Y

D

D

A

K

P

K

T

T

L

L

K

E

I

I

V

L

D

N

L

I

V

A

K

G

K

E

K

Y

R

-

P

-

D

E

L

I

L

P

L

V

L

I

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

D

T

L

F

M

V

T

-

Y

-

E

-

G

-

E

-

H

-

I

-

S

P

P

G

G

F

-

R

-

S

S

L

L

M

T

A

K

E

D

L

V

P

P

E

-

N

-

T

V

I

I

G

V

V

M

Y

N

S

G

Y

L

S
|
S

K
|
K

Y

V

F

Y

G

F

C

A

T

T

G

G

W

W

R
|
R

L

L

G

G

active site: Y125 (≠ F207), D203 (= D289), I205 (≠ V291), K235 (= K318)
binding pyridoxal-5'-phosphate: A99 (= A180), V100 (≠ M181), T101 (≠ C182), Y125 (≠ F207), N175 (= N259), D203 (= D289), I205 (≠ V291), Y206 (= Y292), S234 (= S317), K235 (= K318), R243 (= R326)

Q9C969 Aromatic aminotransferase ISS1; Methionine aminotransferase ISS1; Phenylalanine aminotransferase ISS1; Protein INDOLE SEVERE SENSITIVE 1; Protein REVERSAL OF SAV3 PHENOTYPE 1; Tryptophan aminotransferase ISS1; Tyrosine aminotransferase ISS1; EC 2.6.1.27; EC 2.6.1.5; EC 2.6.1.88 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
37% identity, 17% coverage: 247:335/536 of query aligns to 164:250/394 of Q9C969

query
sites
Q9C969

P

P

K

T

I

P

K

K

A

V

F

V

F

T

V

V

N

N

P

P

G

G

N

N

P

P

T

S

S
x
G

M

T

A

Y

I

V

D

P

A

E

P

P

T

L

L

L

K

K

K

R

I

I

V

A

D

Q

L

I

V

C

K

K

K

D

K

A

R

-

P

-

D

G

L

C

L

W

L

L

L

I

T

V

D

D

D

N

V

T

Y

Y

G

E

T

Y

F

F

V

M

-

Y

P

D

G

G

F

L

R

K

S

H

L

C

M
x
C

A

V

E

E

L

-

P

G

E

D

N

H

T

I

I

V

G

N

V

V

Y

F

S

S

Y

F

S

S

K

K

Y

T

F

Y

G

G

C

M

T

M

G

G

W

W

R

R

L

L

G

G

V

Y

I

I

A

A

I

Y

H

S

E

E

K

R

G179 (≠ S262) mutation to D: In vas1-2; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway. Increases levels of auxin (IAA) and indole-3-pyruvic acid (3-IPA).
C219 (≠ M303) mutation to Y: In vas1-7; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.

Sites not aligning to the query:

66 P→L: In vas1-4; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.
77 L→F: In vas1-5; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.
269 A→T: In vas1-8; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.
362 R→W: In iss1-2; indole-dependent auxin (IAA) overproduction phenotypes including leaf epinasty and adventitious rooting.

1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
27% identity, 29% coverage: 176:328/536 of query aligns to 99:244/382 of 1gc4A

query
sites
1gc4A

G
|
G

T
x
S

A

Q

A

A

M

L

C

F

Y

N

I

L

F

F

K

Q

S

A

L

-

I

-

A

-

N

-

R

-

I

I

L

L

K

D

K

P

G

G

D

D

T

E

I

V

A

I

L

V

G

L

T

S

P

P

I

Y

F
x
W

T

V

P

S

Y

Y

I

P

E

E

I

M

A

V

E

R

L

F

E

A

D

G

Y

G

A

V

F

V

K

-

A

-

V

V

H

E

I

V

K

E

A

T

K

L

Q

P

E

E

N

E

R

G

F

F

Q

V

Y

P

S

D

D

P

E

E

E

R

I

V

K

R

L

A

E

I

D

T

P

P

K

R

I

T

K

K

A

A

F

L

F

V

V

V

V

N

N

S

P

P

G

N

N
|
N

P

P

T

T

S

G

M

A

A

V

I

Y

D

P

A

K

P

E

T

V

L

L

K

E

K

A

I

L

V

A

D

R

L

L

V

A

K

V

K

E

K

H

R

-

P

-

D

D

L

F

L

Y

L

L

L

V

T

S

D
|
D

D

E

V

I

Y
|
Y

G

E

T

H

-

L

-

Y

-

E

-

G

-

E

-

H

F

F

V

S

P

P

G

G

F

R

R

V

S

A

L

-

M

-

A

-

E

-

L

-

P

P

E

E

N

H

T

T

I

L

G

T

V

V

Y

N

S

G

Y

A

S

A

K

K

Y

A

F

F

G

A

C

M

T

T

G

G

W

W

R
|
R

L

I

G

G

binding aspartic acid: W125 (≠ F207), N175 (= N259)
binding pyridoxal-5'-phosphate: G99 (= G176), G100 (= G177), S101 (≠ T178), W125 (≠ F207), N175 (= N259), D203 (= D289), Y206 (= Y292), R242 (= R326)

Sites not aligning to the query:

binding aspartic acid: 64, 261, 322, 361

Query Sequence

>WP_106711968.1 NCBI__GCF_003010955.1:WP_106711968.1
MTTIDYRDYEKMSPFEIKDALIKLAKRSSQKSTQVLLNAGRGNPNWIATKPREGFFLFGQ
FALSEARRALDNPTAGVAGMPQQKDIAERFKVWLEKHAEMEGAEFLSKMVDHAVKAFGFD
ADAFVFELTDSIIGDNYPVPDRMLVHAEQIAHRYLMWAMCGDKVPPGKFDLYAVEGGTAA
MCYIFKSLIANRILKKGDTIALGTPIFTPYIEIAELEDYAFKAVHIKAKQENRFQYSDEE
IKKLEDPKIKAFFVVNPGNPTSMAIDAPTLKKIVDLVKKKRPDLLLLTDDVYGTFVPGFR
SLMAELPENTIGVYSYSKYFGCTGWRLGVIAIHEKNILDSTIAKLPAKDLEALDKRYISI
TLEPRKLKMIDRIVADSRDVALNHTAGLSLPQQVMMTMFSMSELMDTEKLYQKACMDICH
SRVDSLLEGLPLDVQPNPNFAYYYGTIDFEFWLRRYVGEDMVKWLKKNVHPLDLVFKLAE
DHAIVLLNGGGFDAPNWSVRVSFANLTDDVYDDIGRAVRAVGRSYVDAFNASKKGK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory