SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_106712589.1 NCBI__GCF_003010955.1:WP_106712589.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3uboA The crystal structure of adenosine kinase from sinorhizobium meliloti
73% identity, 100% coverage: 2:330/330 of query aligns to 1:329/338 of 3uboA

query
sites
3uboA

S

A

S

K

Y

Y

D

D

V

V

L

L

C

T

I

I

G

G

N
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N

A

A

I
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I

V

V

D
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D

I

I

I

I

A

A

R

R

T

C

D

D

D

D

D

S

F

F

I

L

V

E

K

E

N

N

G

G

I

I

I

I

K

K

N

G

A

A

M
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M

N

N

L

L

I

I

D

N

A

A

D

D

R

R

A

A

E

E

F

L

L

L

Y

Y

E

S

R

R

M

M

G

G

P

P

A

A

I

V

E

E

A

A

S

S

G
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G

G
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G

S
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S

A

A

G

G

N
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N

T

T

A

A

A

A

G

G

I

V

A

A

S

S

L

L

G

G

G

G

R

R

A

A

A

A

Y

Y

F

F

G

G

K

K

V

V

A

A

D

D

D

D

Q

Q

L

L

G

G

H

E

V

I

F

F

T

T

H

H

D

D

I

I

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R

S

A

Q

Q

G

G

V

V

A

H

F

F

D

Q

T

T

R

K

V

P

L

L

N

D

A

G

P

H

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
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R

S

S

M
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M

N

N

T
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T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

D

S

D

K

V

V

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A

E

Q

A

S

K

K

V

V

T

T

Y

Y

F

F

E
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E

G

G

Y
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Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

D

A

A

I

I

R

R

L

E

S

A

A

A

K

R

I

I

A

A

H

H

E

A

H

H

K

G

R

R

E

E

V

T

S

A

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

H

R

R

Y

Y

R

R

E

S

E

E

F

F

L

L

D

E

L

L

M

M

R

R

S

S

G

G

T

T

V

V

D

D

I

I

V

V

F

F

A

A

N
|
N

E

R

A

Q

E

E

L

A

K

L

S

A

L

L

Y

Y

Q

E

T

T

H

E

D

D

F

F

E

D

K

R

G

A

L

L

E

E

L

L

I

L

R

A

K

R

D

D

C

C

K

K

L

L

A

A

A

A

V

V

T
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T

R

L

S
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S

E
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E

K

E

G
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G

S

S

V

V

V

V

V

V

S

R

G

G

D

A

E

E

T

R

V

V

A

R

V

V

P

G

A

A

I

S

E

V

I
x
L

A

E

E

Q

L

V

V

V

D

D

T

T

T

T

G
|
G

A
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A

G
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G

D
|
D

L

L

Y

Y

A

A

A

A

G

G

F

F

L

L

F

F

G

G

Y

Y

T

T

T

S

G

G

R

R

T

S

L

L

N

E

D

E

C

C

A

S

R

K

L

L

G

G

S
x
N

L

L

T

A

A
|
A

G

G

L

I

V

V

I
|
I

Q

G

Q

Q

I

I

G

G

P
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P

R

R

P

P

Q

L

L

V

N

S

L

L

K

A

L

T

A

A

A

A

E

R

Q

Q

A

A

G

A

L

L

L

V

active site: R124 (= R125), G274 (= G275), A275 (= A276), G276 (= G277), D277 (= D278)
binding adenosine: N10 (= N11), I12 (= I13), D14 (= D15), M34 (= M35), G57 (= G58), G58 (= G59), S59 (= S60), N62 (= N63), M126 (= M127), T128 (= T129), E155 (= E156), Y157 (= Y158), G274 (= G275), D277 (= D278), P313 (= P314)
binding adenosine-5'-diphosphate: N214 (= N215), T244 (= T245), S246 (= S247), E247 (= E248), G249 (= G250), L266 (≠ I267), A275 (= A276), G276 (= G277), N301 (≠ S302), A304 (= A305), I308 (= I309)

4k8pA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 2-ethylbenzyl alcohol
71% identity, 100% coverage: 1:330/330 of query aligns to 3:332/332 of 4k8pA

query
sites
4k8pA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A
|
A

E

E

F

L

L

L

Y
|
Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
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G

G
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G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R
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R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
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R

S

S

M
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M

N

N

T
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T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

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E

E

S

A

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D

K

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S

A

E

D

A

A

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K

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V

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T

Y

Y

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E
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E

G

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Y
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Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

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L

D

S

C

A

A

K

R

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I

A

A

H

H

E

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H

H

K

G

R

R

E

E

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M

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S

M

M

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T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

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T

R

M

S

S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
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T

G
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G

A
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A

G
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G

D
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D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R127 (= R125), G277 (= G275), A278 (= A276), G279 (= G277), D280 (= D278)
binding (2-ethylphenyl)methanol: A45 (= A43), Y49 (= Y47), R116 (= R114)
binding adenosine: N13 (= N11), I15 (= I13), D17 (= D15), M37 (= M35), G60 (= G58), G61 (= G59), S62 (= S60), N65 (= N63), M129 (= M127), T131 (= T129), E158 (= E156), Y160 (= Y158), T276 (= T274), D280 (= D278), P316 (= P314)

4lbxA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with cytidine
71% identity, 100% coverage: 1:330/330 of query aligns to 2:331/331 of 4lbxA

query
sites
4lbxA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y

Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
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G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

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L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
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R

S

S

M
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M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
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E

G

G

Y
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Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T
|
T

R

M

S
|
S

E

E

K

N

G
|
G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S
x
C

L

L

T

A

A
|
A

G

G

L

I

V

V

I
|
I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R126 (= R125), G276 (= G275), A277 (= A276), G278 (= G277), D279 (= D278)
binding adenosine: N12 (= N11), I14 (= I13), D16 (= D15), M36 (= M35), G59 (= G58), G60 (= G59), S61 (= S60), N64 (= N63), M128 (= M127), T130 (= T129), E157 (= E156), Y159 (= Y158), T275 (= T274), D279 (= D278), P315 (= P314)
binding 4-amino-1-beta-d-ribofuranosyl-2(1h)-pyrimidinone: T246 (= T245), S248 (= S247), G251 (= G250), C303 (≠ S302), A306 (= A305), I310 (= I309)

4k8kA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 1-(4-methoxyphenyl)-1-cyclopropane and 2- aminoperimidine
71% identity, 100% coverage: 1:330/330 of query aligns to 2:331/331 of 4k8kA

query
sites
4k8kA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A
|
A

D

E

R

R

A
|
A

E
|
E

F

L

L

L

Y
|
Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D
x
A

D
|
D

Q

Q

L

L

G

G

H
x
D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P
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P

T

T

A

A

R
|
R

S

S

M
|
M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
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T

Y
|
Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
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E

G

G

Y
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Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T

T

R

M

S

S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R126 (= R125), G276 (= G275), A277 (= A276), G278 (= G277), D279 (= D278)
binding 1-(4-methoxyphenyl)cyclopropanecarboxylic acid: A41 (= A40), A44 (= A43), E45 (= E44), Y48 (= Y47), P112 (= P111), R115 (= R114), M117 (= M116), Y131 (= Y130)
binding 1H-perimidin-2-amine: A84 (≠ D83), D85 (= D84), D89 (≠ H88)
binding adenosine: N12 (= N11), I14 (= I13), D16 (= D15), M36 (= M35), G59 (= G58), G60 (= G59), S61 (= S60), N64 (= N63), M128 (= M127), T130 (= T129), E157 (= E156), Y159 (= Y158), T275 (= T274), D279 (= D278), P315 (= P314)

4lc4A Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with guanosine
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4lc4A

query
sites
4lc4A

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y

Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T
|
T

R

M

S
|
S

E
|
E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A
|
A

G

G

L

I

V

V

I
|
I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), S60 (= S60), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), T274 (= T274), D278 (= D278), P314 (= P314)
binding guanosine: T245 (= T245), S247 (= S247), E248 (= E248), A305 (= A305), I309 (= I309)

4kbeA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with benzoguanamine
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4kbeA

query
sites
4kbeA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I
|
I

A
x
S

R
|
R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y

Y

E

S

R

R

M

M

G

G

P
|
P

A
|
A

I
x
L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T

T

R

M

S

S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T

T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), S60 (= S60), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), D278 (= D278), P314 (= P314)
binding 6-phenyl-1,3,5-triazine-2,4-diamine: I17 (= I17), S18 (≠ A18), R19 (= R19), P52 (= P52), A53 (= A53), L54 (≠ I54)

4kanA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 2-(2,5-dimethyl-1,3-thiazol-4-yl)acetic acid
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4kanA

query
sites
4kanA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I
|
I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A
|
A

E
|
E

F

L

L

L

Y
|
Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R
|
R

S

S

M
|
M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N
|
N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T
|
T

R

M

S
|
S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), S60 (= S60), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), T274 (= T274), D278 (= D278), P314 (= P314)
binding (2,5-dimethyl-1,3-thiazol-4-yl)acetic acid: I16 (= I16), A43 (= A43), E44 (= E44), Y47 (= Y47), R114 (= R114), M116 (= M116), N215 (= N215), T245 (= T245), S247 (= S247), A276 (= A276)

4kalA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with quinoline-3-carboxylic acid
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4kalA

query
sites
4kalA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y

Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S

S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P
x
S

F
|
F

C

C

V

V

D
|
D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S
|
S

L

L

Y

Y

Q
|
Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T

T

R

M

S

S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P

P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), T274 (= T274), D278 (= D278)
binding quinoline-3-carboxylic acid: S190 (≠ P190), F191 (= F191), D194 (= D194), S221 (= S221), Q224 (= Q224)

4kahA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 4-bromo-1h-pyrazole
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4kahA

query
sites
4kahA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I
|
I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y
|
Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L
|
L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T

T

R

M

S

S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), S60 (= S60), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), T274 (= T274), D278 (= D278), P314 (= P314)
binding 4-bromo-1H-pyrazole: I16 (= I16), Y47 (= Y47), L86 (= L86)

4kadA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with n1-(2.3-dihydro-1h-inden-5-yl)acetam
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4kadA

query
sites
4kadA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y

Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P

S

F

F

C

C

V

V

D

D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q

Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T

T

R

M

S
|
S

E
|
E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A
|
A

G

G

L

I

V

V

I
|
I

Q

Q

Q

Q

I

I

G

G

P

P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding N-(4,7-dihydro-1H-inden-6-yl)acetamide: S247 (= S247), E248 (= E248), A305 (= A305), I309 (= I309)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), S60 (= S60), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), T274 (= T274), D278 (= D278)

4k9iA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with norharmane
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4k9iA

query
sites
4k9iA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y

Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q

Q

L

L

G

G

H

D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R

R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P
x
S

F
|
F

C

C

V

V

D
|
D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q
|
Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T

T

R

M

S

S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P

P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), S60 (= S60), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), T274 (= T274), D278 (= D278)
binding Norharmane: S190 (≠ P190), F191 (= F191), D194 (= D194), Q224 (= Q224)

4k9cA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with n-(hydroxymethyl)benzamide and 4-methyl-3,4- dihydro-2h-1,4-benzoxazine-7-carboxylic acid
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4k9cA

query
sites
4k9cA

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L

L

I

I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y
|
Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q
|
Q

L
|
L

G

G

H
x
D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P
|
P

T

T

A

A

R
|
R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L

L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E

E

S

A

S

D

K

V

V

V

S

A

E

D

A

A

K

K

V

V

T

T

Y

Y

F

F

E
|
E

G

G

Y
|
Y

L

L

W

W

D

D

P

P

P

P

R

R

A

A

K

K

E

E

A

A

I

I

R

L

L

D

S

C

A

A

K

R

I

I

A

A

H

H

E

Q

H

H

K

G

R

R

E

E

V

M

S

S

M

M

T

T

L

L

S

S

D

D

P
x
S

F
|
F

C

C

V

V

D
|
D

R

R

Y

Y

R

R

E

G

E

E

F

F

L

L

D

D

L

L

M

M

R

R

S

S

G

G

T

K

V

V

D

D

I

I

V

V

F

F

A

A

N

N

E

R

A

Q

E

E

L

A

K

L

S

S

L

L

Y

Y

Q
|
Q

T

T

H

D

D

D

F

F

E

E

K

E

G

A

L

L

E

N

L

R

I

I

R

A

K

A

D

D

C

C

K

K

L

I

A

A

A

A

V

V

T

T

R

M

S

S

E

E

K

N

G

G

S

A

V

V

V

I

V

L

S

K

G

G

D

R

E

E

T

R

V

Y

A

Y

V

V

P

N

A

A

I

I

E

R

I

I

A

R

E

E

L

V

V

V

D

D

T

T

T
|
T

G
|
G

A
|
A

G
|
G

D
|
D

L

L

Y

F

A

A

A

S

G

G

F

F

L

L

F

Y

G

G

Y

Y

T

T

T

Q

G

G

R

R

T

S

L

L

N

E

D

D

C

C

A

G

R

K

L

L

G

G

S

C

L

L

T

A

A

A

G

G

L

I

V

V

I

I

Q

Q

Q

Q

I

I

G

G

P
|
P

R

R

P

P

Q

M

L

T

N

S

L

L

K

S

L

E

A

A

A

A

E

K

Q

Q

A

A

G

G

L

L

L

I

active site: R125 (= R125), G275 (= G275), A276 (= A276), G277 (= G277), D278 (= D278)
binding N-(hydroxymethyl)benzamide: Y47 (= Y47), Q85 (= Q85), L86 (= L86), D88 (≠ H88), P111 (= P111), R114 (= R114)
binding adenosine: N11 (= N11), I13 (= I13), D15 (= D15), M35 (= M35), G58 (= G58), G59 (= G59), S60 (= S60), N63 (= N63), M127 (= M127), T129 (= T129), E156 (= E156), Y158 (= Y158), T274 (= T274), D278 (= D278), P314 (= P314)
binding 4-methyl-3,4-dihydro-2H-1,4-benzoxazine-7-carboxylic acid: S190 (≠ P190), F191 (= F191), D194 (= D194), Q224 (= Q224)

4k93A Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with n-(hydroxymethyl)benzamide
71% identity, 100% coverage: 1:330/330 of query aligns to 1:330/330 of 4k93A

query
sites
4k93A

M

M

S

T

S

R

Y

F

D

D

V

V

L

L

C

T

I

V

G

G

N
|
N

A

A

I
|
I

V

V

D
|
D

I

I

I

I

A

S

R

R

T

C

D

N

D

D

D

Q

F

F

I

L

V

I

K

D

N

N

G

Q

I

I

I

T

K

K

N

A

A

A

M
|
M

N

N

L
|
L

I
|
I

D

D

A

A

D

E

R

R

A

A

E

E

F

L

L

L

Y
|
Y

E

S

R

R

M

M

G

G

P

P

A

A

I

L

E

E

A

A

S

S

G
|
G

G
|
G

S
|
S

A

A

G

G

N
|
N

T

T

A

A

A

A

G

G

I

V

A

A

S

N

L

L

G

G

G

G

R

K

A

A

A

A

Y

Y

F

F

G

G

K

N

V

V

A

A

D

A

D

D

Q
|
Q

L

L

G

G

H
x
D

V

I

F

F

T

T

H

H

D

D

I

I

R

R

S

A

Q

Q

G

G

V

V

A

H

F

Y

D

Q

T

T

R

K

V

P

L

K

N

G

A

A

P

F

P

P

P

P

T

T

A

A

R
|
R

S

S

M

M

I

I

F

F

V

V

T

T

P

E

D

D

G

G

E

E

R
|
R

S

S

M
|
M

N

N

T
|
T

Y

Y

L
|
L

G

G

A

A

C

C

I

V

E

E

L

L

G

G

P

P

E

E

D

D

V

V

E