SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_106713177.1 NCBI__GCF_003010955.1:WP_106713177.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
63% identity, 97% coverage: 35:1252/1262 of query aligns to 14:1224/1227 of P13009

query
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P13009

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C247 (= C267) binding Zn(2+)
C310 (= C330) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
C311 (= C331) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
E694 (= E713) binding methylcob(III)alamin
GDVHD 756:760 (= GDVHD 781:785) binding methylcob(III)alamin
D757 (= D782) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H784) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S829) binding methylcob(III)alamin
T808 (= T833) binding methylcob(III)alamin
S810 (= S835) mutation to A: Decreases activity by about 40%.
A860 (= A886) binding methylcob(III)alamin
D946 (= D973) binding S-adenosyl-L-methionine
R1134 (= R1162) binding S-adenosyl-L-methionine
YY 1189:1190 (≠ YF 1217:1218) binding S-adenosyl-L-methionine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
56% identity, 97% coverage: 34:1252/1262 of query aligns to 27:1262/1265 of Q99707

query
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Q99707

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R61 (≠ C68) natural variant: R -> K
C255 (≠ T262) to Y: in dbSNP:rs1140598
GSR 382:384 (≠ GSA 387:389) binding (6S)-5,6,7,8-tetrahydrofolate
D449 (= D454) binding (6S)-5,6,7,8-tetrahydrofolate
N470 (= N475) binding (6S)-5,6,7,8-tetrahydrofolate
D537 (= D542) binding (6S)-5,6,7,8-tetrahydrofolate
N579 (= N584) binding (6S)-5,6,7,8-tetrahydrofolate
R585 (= R590) binding (6S)-5,6,7,8-tetrahydrofolate
R591 (= R596) binding (6S)-5,6,7,8-tetrahydrofolate
D919 (≠ A919) to G: in dbSNP:rs1805087
D963 (≠ N962) mutation to E: Decreases binding to MTRR; when associated with N-1071.
K1071 (= K1062) mutation to N: Decreases binding to MTRR; when associated with E-963.

4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
57% identity, 49% coverage: 34:656/1262 of query aligns to 11:611/611 of 4cczA

query
sites
4cczA

R

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R

K

G

G

L

L

I

L

D

D

G

G

A

V

D

D

I

I

I

L

L

L

I

I

E

E

T

T

I

I

F

F

D

D

T

T

L

A

N

N

A

A

K

K

A

A

I

L

F

F

A

A

T

L

Q

Q

E

N

V

L

F

F

E

E

K

K

G

Y

I

A

T

P

L

R

P

P

I

I

M

F

I

I

S

S

G

G

T

T

I

I

T

V

D

D

R

K

S

S

G

G

R

R

T

T

L

L

S

S

G

G

Q

Q

T

T

P

G

T

E

A

G

F

F

W

V

Y

I

S

S

V

V

R

S

H

H

A

G

K

E

P

P

F

L

T

C

I

I

G

G

L

L

N

N

C

C

A

A

L

L

G

G

A

A

N

A

A

E

M

M

R

R

D

P

H

F

L

I

A

E

E

I

I

I

A

G

S

K

V

C

A

T

D

T

A

A

F

Y

V

V

C

L

A

C

Y

Y

P

P

N

N

A

A

G

G

L

-

P

-

N

-

E

-

F

-

G

-

Q

-

Y

-

D

-

E

-

S

-

P

-

E

-

A

-

M

-

A

-

Q

-

L

-

E

-

F

F

A

A

R

M

E

D

G

G

L

L

L

V

N

N

I

I

V

V

G

G

C

C

G

G

S

S

T

T

P

P

E

D

H

H

I

I

K

R

A

E

I

I

A

A

E

E

A

A

V

V

G

K

K

N

Y

C

G

K

P

P

R

R

E

V

I

P

P

P

E

A

T

T

-

A

-

F

E

E

I

G

H

H

M

M

R

L

L

L

S

S

G

G

L

L

E

E

P

P

F

F

T

R

L

I

T

G

K

P

D

Y

I

T

A

N

F

F

V

V

N

N

V

I

G

G

E
|
E

R

R

T

C

N

N

I

V

T

A

G
|
G

S

S

A
x
R

K

K

F

F

R

A

K

K

L

L

I

I

T

M

S

A

G

G

D

N

F

Y

A

E

T

E

A

A

L

L

D

C

V

V

A

A

R

K

D

V

Q

Q

V

V

A

E

N

M

G

G

A

A

Q

Q

I

V

I

L

D

D

I

V

N

N

M

M

D

D

E

D

G

G

L

M

I

L

D

D

S

G

E

P

K

S

A

A

M

M

V

T

E

R

F

F

L

C

N

N

L

L

I

I

A

A

A

S

E

E

P

P

D

D

I

I

A

A

R

K

V

V

P

P

V

L

M

C

I

I

D

D

S

S

K

N

W

F

D

A

V

V

I

I

E

E

A

A

G

G

L

L

K

K

C

C

V

C

Q

Q

G

G

K

K

P

C

L

I

V

V

N
|
N

S

S

I

I

S

S

M

L

K

K

E

E

G

G

E

E

Q

D

A

D

F

F

I

L

D

E

H

K

A

A

K

R

R

K

V

I

R

K

A

K

Y

Y

G

G

A

A

V

M

V

V

M
|
M

A

A

F

F

D

D

T

E

Q

E

G

G

Q

Q

A

A

D

T

T

E

L

T

E

D

R

T

K

K

V

I

A

R

I

V

S

C

T

T

R

R

A

A

Y

Y

E

H

L

L

T

V

R

K

E

K

A

L

G

G

F

F

P

N

P

P

E

N

D

D

I

I

V

I

F

F

D
|
D

P

P

N

N

V

I

F

L

A

T

V

I

A

G

T

T

G

G

I

M

E

E

H

H

N

N

G

L

Y

Y

G

A

V

I

A

N

F

F

I

I

E

H

A

A

T

T

R

K

Q

V

I

I

T

K

E

E

T

T

L

L

P

P

H

G

V

A

H

R

I

I

S

S

G

G

G
|
G

I

L

S
|
S

N
|
N

L

L

S

S

F
|
F

S

S

F

F

R
|
R

G

G

N

M

E

E

P

A

V

I

R
|
R

E

E

A

A

M

M

H

H

A

G

V

V

F

F

L

L

Y

Y

H

H

A

A

I

I

Q

K

V

S

G

G

M

M

D

D

M

M

G

G

I

I

V

V

N

N

A

A

G

G

Q

N

L

L

A

P

V

V

Y

Y

E

D

S

D

I

I

D

H

P

K

E

E

L

L

R

L

E

Q

A

L

C

C

E

E

D

D

V

L

V

I

L

W

N

N

R

K

R

D

D

P

D

E

A

A

T

T

E

E

R

K

L

L

D

R

L

Y

A

A

E

Q

binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E381), G342 (= G387), R344 (≠ A389), N430 (= N475), M458 (= M503), D497 (= D542), G536 (= G581), S538 (= S583), N539 (= N584), F542 (= F587), R545 (= R590), R551 (= R596)

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
61% identity, 46% coverage: 673:1252/1262 of query aligns to 4:574/577 of 3bulA

query
sites
3bulA

W

W

R

R

E

S

W

W

S

E

V

V

E

N

K

K

R

R

I

L

E

E

H

Y

A

S

L

L

V

V

N

K

G

G

I

I

T

T

E

E

F

F

I

I

E

E

A

Q

D

D

T

T

E

E

A

A

R

R

L

Q

E

Q

A

A

E

T

R

R

P

P

L

C

H

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

A

D

G

G

M

M

N

N

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

V

Y

L

L

L

E

P

P

Y

F

M

I

E

E

A

A

E

S

K

K

L

-

A

-

N

-

G

-

S

-

G

E

Q

Q

R

C

E

K

S

T

A

N

G

G

K

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G

G

K

K

N

N

I

I

V
|
V

G

G

V

V

L

L

A

Q

C

C

N

N

Y

Y

E

E

I

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

S

A

A

E

K

K

I

I

L

L

Q

R

T

T

A

A

R

K

D

E

E

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

H

N

V

V

A

A

A

K

E

E

M

M

E

E

R

R

E

Q

G

G

F

F

D

T

I

I

P

P

L

L

I

I

G
|
G

A

A

T

T

S

S

R

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

N

E

P

Q

R

N

Y

Y

H

-

K

S

G

G

Q

P

T

T

V

V

Y

Y

V

V

T
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

S

A

N

A

L

L

S

S

L

D

E

T

A

Q

K

R

P

D

G

D

Y

F

V

V

D

A

G

R

V

T

R

R

A

K

E

E

Y

Y

T

E

K

T

V

V

A

R

E

I

A

Q

H

H

A

G

R

R

N

K

E

K

A

P

D

R

K

T

Q

P

R

P

L

V

S

T

L

L

A

E

K

A

A

A

R

R

A

D

N

N

A

D

Q

F

M

A

V

F

D

D

W

W

V

Q

S

A

Y

Y

T

T

P

P

K

V

P

A

S

H

F

R

L

L

G

G

T

V

R

Q

V

E

F

V

E

E

N

A

Y

-

D

S

L

I

A

E

E

T

I

L

A

R

R

N

Y

Y

I

I

D

D

W

W

T

T

P

P

F

F

Q
x
M

T

T

W

W

E

S

L

L

K

A

G

G

R

K

Y

Y

P

P

A

R

I

I

L

L

E

E

D

D

E

E

K

V

Q

V

G

G

P

V

A

E

A

A

R

Q

Q

R

L

L

F

F

D

K

D

D

A

A

Q

N

A

D

M

M

L

L

K

D

K

K

I

L

I

S

A

A

E

E

K

K

W

T

F

L

N

N

P

P

K

R

A

G

V

V

I

V

G

G

L

L

W

F

P

P

A

A

G

N

A

R

V

V

G

G

D

D

I

I

R

E

L

I

F

Y

T

R

D

D

E

E

A

T

R

R

G

T

Q

H

E

V

L

I

A

N

T

V

F

S

F

H

T

H

L

L

R

R

Q

Q

L

-

S

T

K

E

R

K

D

T

G

G

R

F

P

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

V

K

D

L

S

S

G

G

K

K

E

A

D

D

Y

Y

V

I

G

G

G

A

F

F

V

A

V

V

T

T

A

G

G

G

I

L

E

E

V

D

A

A

I

L

A

A

E

D

R

A

F

F

E

E

R

A

A

Q

N

H

D

D

Y

Y

S

N

S

K

I

I

L

M

V

V

K

K

A

A

L

L

A

A

D
|
D

R

R

F

L

A

A

E

E

A

A

F

F

A

A

E

E

L

Y

M

L

H

H

Q

E

R

R

V

V

R

R

K

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

P

P

E

N

E

E

N

N

L

L

A

S

S

N

D

E

L

L

I

I

G

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R

R

P

P

A
|
A

P
|
P

G
|
G

Y
|
Y

P

P

A

A

Q

C

P

P

D

E

H
|
H

T

T

E

E

K
|
K

E

A

T

T

L

I

F

W

R

E

L

L

D

E

A

V

E

E

A

K

K

H

I

T

D

G

V

M

R

K

L

L

T

T

E

E

S

S

F

F

A

A

M
|
M

W

W

P

P

G
|
G

S

A

S

S

V
|
V

S
|
S

G

G

I

W

Y

Y

I

F

A

S

H

H

P

P

D

D

S

S

Y

K

Y

Y

F

Y

G

A

V

V

A

A

K

Q

V

I

E

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

A

R

R

R

K

K

S

G

M

M

P

S

V

V

A

T

D

E

V

V

E

E

R

R

W

W

L

L

G

A

P

P

I

N

L

L

N

G

Y

Y

active site: D107 (= D782), H109 (= H784), S160 (= S835)
binding cobalamin: H109 (= H784), V116 (= V791), G152 (= G827), L153 (= L828), S154 (= S829), L156 (= L831), I157 (= I832), T158 (= T833), G183 (= G858), G184 (= G859), Q208 (≠ T884), N209 (≠ D885), A210 (= A886), T213 (≠ A889), M302 (≠ Q979), D443 (= D1121), A486 (= A1164), P487 (= P1165), G488 (= G1166), Y489 (= Y1167), H495 (= H1173), K498 (= K1176), M521 (= M1199), G524 (= G1202), V527 (= V1205), S528 (= S1206)

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
61% identity, 46% coverage: 673:1252/1262 of query aligns to 4:574/576 of 3ivaA

query
sites
3ivaA

W

W

R

R

E

S

W

W

S

E

V

V

E

N

K

K

R

R

I

L

E

E

H

Y

A

S

L

L

V

V

N

K

G

G

I

I

T

T

E

E

F

F

I

I

E

E

A

Q

D

D

T

T

E

E

A

A

R

R

L

Q

E

Q

A

A

E

T

R

R

P

P

L

C

H

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

A

D

G

G

M

M

N

N

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

V

Y

L

L

L

E

P

P

Y

F

M

I

E

E

A

A

E

S

K

K

L

-

A

-

N

-

G

-

S

-

G

E

Q

Q

R

C

E

K

S

T

A

N

G

G

K

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

I

V
|
V

G

G

V

V

L

L

A

Q

C

C

N

N

Y

Y

E

E

I

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

S

A

A

E

K

K

I

I

L

L

Q

R

T

T

A

A

R

K

D

E

E

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

H

N

V

V

A

A

A

K

E

E

M

M

E

E

R

R

E

Q

G

G

F

F

D

T

I

I

P

P

L

L

I

I

G
|
G

A

A

T

T

S

S

R

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

N

E

P

Q

R

N

Y

Y

H

-

K

S

G

G

Q

P

T

T

V

V

Y

Y

V

V

T
x
Q

D
x
N

A

A

S

S

R

R

A

T

V

V

G

G

V

V

S

A

N

A

L

L

S

S

L

D

E

T

A

Q

K

R

P

D

G

D

Y

F

V

V

D

A

G

R

V

T

R

R

A

K

E

E

Y

Y

T

E

K

T

V

V

A

R

E

I

A

Q

H

H

A

G

R

R

N

K

E

K

A

P

D

R

K

T

Q

P

R

P

L

V

S

T

L

L

A

E

K

A

A

A

R

R

A

D

N

N

A

D

Q

F

M

A

V

F

D

D

W

W

V

Q

S

A

Y

Y

T

T

P

P

K

V

P

A

S

H

F

R

L

L

G

G

T

V

R

Q

V

E

F

V

E

E

N

A

Y

-

D

S

L

I

A

E

E

T

I

L

A

R

R

N

Y

Y

I

I

D

D

W

W

T

T

P

P

F

F

Q

M

T
|
T

W

W

E

S

L

L

K

A

G

G

R

K

Y

Y

P

P

A

R

I

I

L

L

E

E

D

D

E

E

K

V

Q

V

G

G

P

V

A

E

A

A

R

Q

Q

R

L

L

F

F

D

K

D

D

A

A

Q

N

A

D

M

M

L

L

K

D

K

K

I

L

I

S

A

A

E

E

K

K

W

T

F

L

N

N

P

P

K

R

A

G

V

V

I

V

G

G

L

L

W

F

P

P

A

A

G

N

A

R

V

V

G

G

D

D

I

I

R

E

L

I

F

Y

T

R

D

D

E

E

A

T

R

R

G

T

Q

H

E

V

L

I

A

N

T

V

F

S

F

H

T

H

L

L

R

R

Q

Q

L

-

S

T

K

E

R

K

D

T

G

G

R

F

P

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

V

K

D

L

S

S

G

G

K

K

E

A

D

D

Y

Y

V

I

G

G

G

A

F

F

V

A

V

V

T

T

A

G

G

G

I

L

E

E

V

D

A

A

I

L

A

A

E

D

R

A

F

F

E

E

R

A

A

Q

N

H

D

D

Y

Y

S

N

S

K

I

I

L

M

V

V

K

K

A

A

L

L

A

A

D
|
D

R

R

F

L

A

A

E
|
E

A

A

F

F

A

A

E

E

L

Y

M

L

H

H

Q

E

R

R

V

V

R

R

K

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

P

P

E

N

E

E

N

N

L

L

A

S

S

N

D

E

L

L

I

I

G

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G
|
G

Y
|
Y

P

P

A
|
A

Q

C

P

P

D

E

H
|
H

T

T

E

E

K

K

E

A

T

T

L

I

F

W

R

E

L

L

D

E

A

V

E

E

A

K

K

H

I

T

D

G

V

M

R

K

L

L

T

T

E

E

S

S

F

F

A
|
A

M
|
M

W

W

P

P

G
|
G

S

A

S

S

V
|
V

S
|
S

G

G

I

W

Y

Y

I

F

A

S

H

H

P

P

D

D

S

S

Y

K

Y
|
Y

F

Y

G

A

V

V

A

A

K

Q

V

I

E

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

A

R

R

R

K

K

S

G

M

M

P

S

V

V

A

T

D

E

V

V

E

E

R

R

W

W

L

L

G

A

P

P

I

N

L

L

N

G

Y

Y

active site: D107 (= D782), H109 (= H784), S160 (= S835)
binding cobalamin: H109 (= H784), G112 (= G787), V116 (= V791), G152 (= G827), L153 (= L828), S154 (= S829), L156 (= L831), I157 (= I832), T158 (= T833), G183 (= G858), G184 (= G859), Q208 (≠ T884), N209 (≠ D885), T303 (= T980), D443 (= D1121), A486 (= A1164), G488 (= G1166), Y489 (= Y1167), H495 (= H1173), A520 (= A1198), M521 (= M1199), G524 (= G1202), V527 (= V1205), S528 (= S1206)
binding s-adenosyl-l-homocysteine: E447 (= E1125), R484 (= R1162), P485 (= P1163), Y489 (= Y1167), A491 (= A1169), Y539 (= Y1217)

8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
35% identity, 66% coverage: 31:867/1262 of query aligns to 7:806/841 of 8g3hA

query
sites
8g3hA

K

R

A

A

A

L

R

S

E

Q

R

R

I

V

L

L

I

V

L

F

D

D

G

G

A

A

M

M

G

G

T

T

Q

E

I

L

Q

Q

G

K

L

R

N

D

L

L

N

T

E

P

E

E

S

D

F

Y

R

G

G

E

D

E

R

A

F

Y

A

Y

D

G

C

C

S

P

C

-

H

-

L

-

Q

-

G

-

N

-

D

E

L

V

L

L

I

N

L

R

T

T

Q

R

P

P

Q

D

A

V

I

V

E

R

D

E

I

I

H

H

Y

L

A

S

Y

Y

A

L

M

E

A

A

G

G

A

A

D

E

I

V

L

I

E

E

T

T

N

N

T

S

F

F

S

G

S

A

T

L

T

R

I

H

A

V

Q

L

A

A

D

E

Y

Y

S

G

M

L

E

G

E

E

V

E

V

A

Y

E

E

E

L

L

N

A

R

Y

D

R

G

A

A

A

R

R

L

I

A

A

R

K

R

E

A

A

I

-

K

-

A

-

E

-

Q

-

K

-

D

E

G

P

K

Y

R

G

R

A

F

F

V

V

A

A

G

G

A

A

L

L

G

G

P

P

T

G

N

T

R

K

T

L

A

V

S

S

I

L

S

G

P

-

D

-

V

-

N

-

P

-

G

-

Y

-

R

-

A

Q

V

I

T

S

F

W

D

E

L

L

R

F

I

S

A

A

Y

Y

A

K

E

E

Q

A

V

V

R

R

G

G

L

L

I

V

D

R

G

G

A

V

D

D

I

L

I

I

L

L

I

L

E

E

T

T

I

A

F

Q

D

D

T

I

L

L

N

Q

A

V

K

R

A

C

A

A

I

V

F

L

A

A

T

A

Q

R

E

E

V

A

F

M

E

A

E

E

K

L

G

G

I

V

T

E

L

L

P

P

I

L

M

Q

I

V

S

Q

G

V

T

T

I

F

T

-

D

E

R

A

S

T

G

G

R

T

T

M

L

L

S

V

G

G

Q

T

T

D

P

E

T

Q

A

A

F

A

W

L

Y

A

S

A

V

L

R

E

H

S

A

L

K

P

P

I

F

D

T

V

I

V

G

G

L

M

N

N

C

C

A

A

L

T

G

G

A

P

N

D

A

L

M

M

R

D

D

A

H

K

L

I

A

R

E

Y

I

F

A

A

S

Q

V

E

A

S

D

T

A

R

F

F

V

V

C

A

A

C

Y

L

P

P

N

N

A

A

G

G

L

L

P

P

-

R

N

N

E

E

F

G

G

G

Q

R

-

V

Y

Y

D

D

E

L

S

T

P

P

E

E

A

E

M

L

A

A

A

R

Q

W

L

H

E

L

E

K

F

F

A

V

R

T

E

E

G

Y

L

G

L

V

N

N

I

A

V

V

G

G

C

C

G

G

S

T

T

G

P

P

E

E

H

H

I

I

K

R

A

K

I

V

A

A

E

E

A

A

V

V

G

G

K

G

Y

R

G

-

P

P

R

S

E

P

I

V

P

H

E

K

T

T

E

A

I

F

H

P

M

P

R

Q

L

V

S

A

G

S

L

L

-

Y

E

Q

P

A

F

V

T

P

L

L

T

R

K
x
Q

D

E

I
x
T

A
x
S

F

L

V

L

N

L

V

V

G

G

E

E

R

R

T

L

N

N

I

A

T

T

G

G

S

S

A

K

K

R

F

F

R

R

K

E

L

L

I

L

T

F

S

A

G

G

D

D

F

L

A

E

T

G

A

I

L

L

D

A

V

L

A

A

R

Q

D

E

Q

Q

V

V

A

A

N

E

G

G

A

A

Q

H

I

V

I

L

D

D

I

L

N

S

M

V

D

A

E

W

G

T

L

G

I

R

D

D

S

E

E

L

K

E

A

D

M

M

V

K

E

R

F

V

L

L

N

S

L

K

I

L

A

A

A

T

E

G

P

-

D

-

I

-

A

V

R

T

V

V

P

P

V

F

M

M

I

V

D

D

S

S

S

T

K

S

W

P

D

E

V

V

I

M

E

E

A

E

G

A

L

L

K

K

C

R

V

L

Q

P

G

G

K

R

P

A

L

I

V

L

N

N

S

S

I

A

S

N

M

L

K

E

E

D

G

G

E

L

Q

E

A

K

F

F

I

D

D

R

H

V

A

A

K

S

R

L

V

A

R

K

A

A

Y

H

G

G

A

A

V

L

V

V

V

A

M

L

A

A

F

I

D

D

T

E

Q

E

G

G

Q

M

A

A

D

K

T

T

L

R

E

V

R

K

K

K

V

V

A

E

I

V

S

A

T

L

R

R

A

M

Y

Y

E

E

L

R

L

L

T

T

R

E

E

H

A

H

G

G

F

L

P

R

P

P

E

E

D

D

I

L

V

L

F

F

D

D

P

L

N

L

V

T

F

F

A

P

V

I

A

T

T

Q

G

G

I

D

E

E

H

T

N

R

G

P

Y

L

G

A

V

R

A

E

F

T

I

L

E

L

A

A

T

L

R

E

Q

E

I

L

T

R

E

E

T

R

L

L

P

P

H

G

V

V

H

G

I

F

S

V

G

L

G

G

I

V

S

S

N

N

L

V

S

S

F

F

S

G

F

L

R

K

G

P

N

K

E

-

P

-

V

A

R

R

E

R

A

V

M

L

H

N

A

S

V

V

F

F

L

L

Y

D

H

E

A

A

I

R

Q

K

V

K

G

G

M

L

D

T

M

A

G

A

I

I

V

V

N

D

A

A

G

G

Q

K

L

I

A

L

V

P

Y

I

E

S

S

Q

I

I

D

P

P

E

E

E

L

A

R

Y

E

A

A

L

C

A

E

L

D

D

V

L

V

I

L

Y

N

D

R

R

D

E

-

G

-

Q

D

D

A

P

T

L

E

F

R

A

L

F

L

I

D

R

L

F

A

F

E

E

R

E

Y

H

K

K

G

E

A

V

A

L

G

A

K

E

E

D

A

K

R

E

E

-

R

-

D

-

L

-

A

A

W

F

R

Q

E

A

W

L

S

P

V

V

E

E

K

E

R

R

I

L

E

R

H

R

A

R

L

V

V

L

N

E

G

G

I

K

T

R

E

V

F

G

I

L

E

E

A

E

D

D

T

L

E

A

E

E

A

A

R

-

L

L

E

G

A

R

E

M

R

R

P

P

L

L

H

E

V

I

I

I

E

N

G

G

P

P

L

L

M

L

A

E

G

A

M

M

N

K

V

E

V

V

G

G

D

E

L

L

F
|
F

G

G

A

A

G

G

K

K

M

M

F

Q

L

L

P

P

Q

F

V
|
V

V

L

K

Q

S

A

A

A

R

E

V

V

M

M

K
|
K

Q

Q

A

A

V

V

A

A

V

Y

L

L

L

E

P

P

Y

H

M

M

E

E

A

K

E

K

K

-

L

-

A

-

N

-

G

-

G

G

S

E

G

G

Q

K

R

-

E

-

S

-

A

-

G

G

K

K

V

L

L

V

M

L

A

A

T

T

V

V

K

K

G
|
G

D

D

V
|
V

H
|
H

D
|
D

I
|
I

G
|
G

K

K

N

N

I

L

V
|
V

G

D

V

I

L

L

A

T

C

N

N

N

N

G

Y

Y

E

T

I

V

I

Y

D

N

L

L

G

G

V

I

M

K

V

V

P

P

S

I

A

E

K

E

I

M

L

L

Q

K

T

A

A

V

R

D

D

E

E

V

K

K

V

P

D

H

I

A

I

L

G

G

L
x
M

S
|
S

G

G

L
|
L

I

L

T
x
V

P

K

S

S

L

T

D

Q

E

V

M

M

V

K

H

E

V

N

A

L

A

E

E

Y

M

M

E

R

R

A

E

R

G

G

F

Y

D

T

I

L

P

P

L

V

L
x
I

I
x
L

G
|
G

A
|
A

T

A

T

L

S

T

R

R

V

A

H

Y

T

V

binding cobalamin: Q328 (≠ K372), T330 (≠ I374), S331 (≠ A375), F675 (= F727), V685 (= V737), K693 (= K745), G720 (= G781), V722 (= V783), H723 (= H784), D724 (= D785), I725 (= I786), G726 (= G787), V730 (= V791), M767 (≠ L828), S768 (= S829), L770 (= L831), V772 (≠ T833), I795 (≠ L856), L796 (≠ I857), G797 (= G858), G798 (= G859), A799 (= A860)

Sites not aligning to the query:

binding cobalamin: 818, 819, 820, 821

3k13C Structure of the pterin-binding domain metr of 5- methyltetrahydrofolate-homocysteine methyltransferase from bacteroides thetaiotaomicron
63% identity, 23% coverage: 373:659/1262 of query aligns to 1:286/287 of 3k13C

query
sites
3k13C

D

E

I

I

A

N

F

F

V

V

N

N

V

I

G

G

E
|
E

R

R

T

C

N

N

I

V

T

A

G
|
G

S

S

A
x
R

K

K

F

F

R

L

K

R

L

L

I

V

T

N

S

E

G

K

D

K

F

Y

A

D

T

E

A

A

L

L

D

S

V

I

A

A

R

R

D

Q

Q

Q

V

V

A

E

N

D

G

G

A

A

Q

L

I

V

I

I

D

D

I

V

N

N

M

M

D

D

E

D

G

G

L

L

I

L

D

D

S

A

E

R

K

T

A

E

M

M

V

T

E

T

F

F

L

L

N

N

L

L

I

I

A

M

A

S

E

E

P

P

D

E

I

I

A

A

R

R

V

V

P

P

V

V

M

M

I

I

D

D

S

S

K

K

W

W

D

E

V

V

I

I

E

E

A

A

G

G

L

L

K

K

C

C

V

L

Q

Q

G

G

K

K

P

S

L

I

V

V

N
|
N

S

S

I

I

S

S

M

L

K

K

E

E

G

G

E

E

Q

E

A

V

F

F

I

L

D

E

H

H

A

A

K

R

R

I

V

I

R

K

A

Q

Y

Y

G

G

A

A

V

T

V

V

M

M

A

A

F

F

D

D

T

E

Q

K

G

G

Q

Q

A

A

D

D

T

T

L

A

E

A

R

R

K

K

V

I

A

E

I

V

S

C

T

E

R

R

A

A

Y

Y

E

R

L

L

T

V

R

D

E

K

A

V

G

G

F

F

P

N

P

P

E

H

D

D

I

I

V

I

F

F

D
|
D

P

P

N

N

V

V

F

L

A

A

V

V

A

A

T

T

G

G

I

I

E

E

H

H

N

N

G

N

Y

Y

G

A

V

V

A

D

F

F

I

I

E

E

A

A

T

T

R

G

Q

W

I

I

T

R

E

K

T

N

L

L

P

P

H

G

V

A

H

H

I

V

S

S

G

G

G
|
G

I

V

S
|
S

N
|
N

L

L

S

S

F

F

S

S

F

F

R
|
R

G

G

N

N

E

N

P

Y

V

I

R
|
R

E

E

A

A

M

M

H

H

A

A

V

V

F

F

L

L

Y

Y

H

H

A

A

I

I

Q

Q

V

Q

G

G

M

M

D

D

M

M

G

G

I
|
I

V

V

N

N

A

P

G

G

Q

S

L

V

A

-

V

L

Y

Y

E

S

S

D

I

I

D

P

P

A

E

D

L

T

R

L

E

E

A

K

C

I

E

E

D

D

V

V

L

L

N

N

R

R

D

P

D

D

A

A

T

A

E

E

R

R

L

L

L

I

D

E

L

L

A

A

E

E

R

A

Y

L

K

K

binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E9 (= E381), G15 (= G387), R17 (≠ A389), N103 (= N475), D170 (= D542), G209 (= G581), S211 (= S583), N212 (= N584), R218 (= R590), R224 (= R596), I244 (= I616)

6bdyA Crystal structure of the meth reactivation domain bound to sinefungin (see paper)
56% identity, 26% coverage: 930:1252/1262 of query aligns to 4:324/326 of 6bdyA

query
sites
6bdyA

L

V

S

T

L

L

A

E

K

A

A

A

R

R

A

D

N

N

A

D

Q

F

M

A

V

F

D

D

W

W

V

Q

S

A

Y

Y

T

T

P

P

K

V

P

A

S

H

F

R

L

L

G

G

T

V

R

Q

V

E

F

V

E

E

N

A

Y

-

D

S

L

I

A

E

E

T

I

L

A

R

R

N

Y

Y

I

I

D
|
D

W

W

T

T

P

P

F

F

Q

M

T

T

W

W

E

S

L

L

K

A

G

G

R

K

Y

Y

P

P

A

R

I

I

L

L

E

E

D

D

E

E

K

V

Q

V

G

G

P

V

A

E

A

A

R

Q

Q

R

L

L

F

F

D

K

D

D

A

A

Q

N

A

D

M

M

L

L

K

D

K

K

I

L

I

S

A

A

E

E

K

K

W

T

F

L

N

N

P

P

K

R

A

G

V

V

I

V

G

G

L

L

W

F

P

P

A

A

G

N

A

R

V

V

G

G

D

D

I

I

R

E

L

I

F

Y

T

R

D

D

E

E

A

T

R

R

G

T

Q

H

E

V

L

I

A

N

T

V

F

S

F

H

T

H

L

L

R

R

Q

Q

L

-

S

T

K

E

R

K

D

T

G

G

R

F

P

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

V

K

D

L

S

S

G

G

K

K

E

A

D

D

Y

Y

V

I

G

G

G

A

F

F

V

A

V

V

T

T

A

G

G

G

I

L

E

E

V

D

A

A

I

L

A

A

E

D

R

A

F

F

E

E

R

A

A

Q

N

H

D

D

Y

Y

S

N

S

K

I

I

L

M

V

V

K

K

A

A

L

L

A

A

D

D

R

R

F

L

A

A

E

E

A

A

F

F

A

A

E

E

L

Y

M

L

H

H

Q

E

R

R

V

V

R

R

K

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

P

P

E

N

E

E

N

N

L

L

A

S

S

N

D

E

L

L

I

I

G

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G

G

Y
|
Y

P
|
P

A
|
A

Q

C

P

P

D

E

H

H

T

T

E

E

K

K

E

A

T

T

L

I

F

W

R

E

L

L

D

E

A

V

E

E

A

K

K

H

I

T

D

G

V

M

R

K

L

L

T

T

E

E

S

S

F

F

A

A

M

M

W

W

P

P

G

G

S

A

S

S

V

V

S

S

G

G

I

W

Y

Y

I

F

A

S

H

H

P

P

D

D

S

S

Y

K

Y
|
Y

F

Y

G

A

V

V

A

A

K

Q

V

I

E

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

A

R

R

R

K

K

S

G

M

M

P

S

V

V

A

T

D

E

V

V

E

E

R

R

W

W

L

L

G

A

P

P

I

N

L

L

N

G

Y

Y

binding sinefungin: D46 (= D973), R234 (= R1162), P235 (= P1163), A236 (= A1164), Y239 (= Y1167), P240 (= P1168), A241 (= A1169), Y289 (= Y1217)

1mskA Methionine synthase (activation domain) (see paper)
56% identity, 26% coverage: 930:1252/1262 of query aligns to 4:324/327 of 1mskA

query
sites
1mskA

L

V

S

T

L

L

A

E

K

A

A

A

R

R

A

D

N

N

A

D

Q

F

M

A

V

F

D

D

W

W

V

Q

S

A

Y

Y

T

T

P

P

K

V

P

A

S

H

F

R

L

L

G

G

T

V

R

Q

V

E

F

V

E

E

N

A

Y

-

D

S

L

I

A

E

E

T

I

L

A

R

R

N

Y

Y

I

I

D
|
D

W

W

T

T

P

P

F

F

Q

M

T

T

W

W

E

S

L

L

K

A

G

G

R

K

Y

Y

P

P

A

R

I

I

L

L

E

E

D

D

E

E

K

V

Q

V

G

G

P

V

A

E

A

A

R

Q

Q

R

L

L

F

F

D

K

D

D

A

A

Q

N

A

D

M

M

L

L

K

D

K

K

I

L

I

S

A

A

E

E

K

K

W

T

F

L

N

N

P

P

K

R

A

G

V

V

I

V

G

G

L

L

W

F

P

P

A

A

G

N

A

R

V

V

G

G

D

D

I

I

R

E

L

I

F

Y

T

R

D

D

E

E

A

T

R

R

G

T

Q

H

E

V

L

I

A

N

T

V

F

S

F

H

T

H

L

L

R

R

Q

Q

L

-

S

T

K

E

R

K

D

T

G

G

R

F

P

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

V

K

D

L

S

S

G

G

K

K

E

A

D

D

Y

Y

V

I

G

G

G

A

F

F

V

A

V

V

T

T

A

G

G

G

I

L

E

E

V

D

A

A

I

L

A

A

E

D

R

A

F

F

E

E

R

A

A

Q

N

H

D

D

Y

Y

S

N

S

K

I

I

L

M

V

V

K

K

A

A

L

L

A

A

D

D

R

R

F

L

A

A

E

E

A

A

F

F

A

A

E

E

L

Y

M

L

H

H

Q

E

R

R

V

V

R

R

K

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

P

P

E

N

E

E

N

N

L

L

A

S

S

N

D

E

L

L

I

I

G

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A

A

P

P

G

G

Y
|
Y

P

P

A
|
A

Q

C

P

P

D

E

H

H

T

T

E

E

K

K

E

A

T

T

L

I

F

W

R

E

L

L

D

E

A

V

E

E

A

K

K

H

I

T

D

G

V

M

R

K

L

L

T

T

E

E

S

S

F

F

A

A

M

M

W

W

P

P

G

G

S

A

S

S

V

V

S

S

G

G

I

W

Y

Y

I

F

A

S

H

H

P

P

D

D

S

S

Y

K

Y
|
Y

F

Y

G

A

V

V

A

A

K

Q

V

I

E

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

A

R

R

R

K

K

S

G

M

M

P

S

V

V

A

T

D

E

V

V

E

E

R

R

W

W

L

L

G

A

P

P

I

N

L

L

N

G

Y

Y

binding s-adenosylmethionine: D46 (= D973), R234 (= R1162), P235 (= P1163), Y239 (= Y1167), A241 (= A1169), Y289 (= Y1217)

1bmtA How a protein binds b12: a 3.O angstrom x-ray structure of the b12- binding domains of methionine synthase (see paper)
68% identity, 20% coverage: 673:922/1262 of query aligns to 4:246/246 of 1bmtA

query
sites
1bmtA

W

W

R

R

E

S

W

W

S

E

V

V

E

N

K

K

R

R

I

L

E

E

H

Y

A

S

L

L

V

V

N

K

G

G

I

I

T

T

E

E

F

F

I

I

E

E

A

Q

D

D

T

T

E

E

A

A

R

R

L

Q

E

Q

A

A

E

T

R

R

P

P

L

I

H

E

V

V

I

I

E
|
E

G

G

P

P

L

L

M
|
M

A

D

G

G

M
|
M

N

N

V

V

G
|
G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L
|
L

P

P

Q

Q

V
|
V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

V

Y

L

L

L

E

P

P

Y

F

M

I

E

E

A

A

E

S

K

K

L

-

A

-

N

-

G

-

S

-

G

E

Q

Q

R

G

E

K

S

T

A

N

G

G

K

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V
|
V

H
|
H

D
|
D

I
|
I

G

G

K

K

N

N

I
|
I

V

V

G

G

V

V

L

L

A

Q

C

C

N

N

Y

Y

E

E

I

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

S

A

A

E

K

K

I

I

L

L

Q

R

T

T

A

A

R

K

D

E

E

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

H

N

V

V

A

A

A

K

E

E

M

M

E

E

R

R

E

Q

G

G

F

F

D

T

I

I

P

P

L

L

I

I

G
|
G

A
|
A

T

T

S

S

R

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

N

E

P

Q

R

N

Y

Y

H

-

K

S

G

G

Q

P

T

T

V

V

Y

Y

V
|
V

T

Q

D
x
N

A
|
A

S

S

R

R

A

T

V

V

G

G

V

V

S

A

N

A

L

L

S

S

L

D

E

T

A

Q

K

R

P

D

G

D

Y

F

V

V

D

A

G

R

V

T

R

R

A

K

E

E

Y

Y

T

E

K

T

V

V

A

R

E

I

A

Q

H

H

A

G

R

R

active site: D107 (= D782), H109 (= H784), S160 (= S835)
binding co-methylcobalamin: E44 (= E713), M48 (= M717), M51 (= M720), G55 (= G724), L65 (= L734), V68 (= V737), D107 (= D782), V108 (= V783), H109 (= H784), D110 (= D785), I111 (= I786), I115 (= I790), G152 (= G827), L153 (= L828), S154 (= S829), L156 (= L831), I157 (= I832), T158 (= T833), G183 (= G858), G184 (= G859), A185 (= A860), V207 (= V883), N209 (≠ D885), A210 (= A886)

8sseA Methionine synthase, c-terminal fragment, cobalamin and reactivation domains from thermus thermophilus hb8 (see paper)
33% identity, 43% coverage: 680:1220/1262 of query aligns to 4:506/507 of 8sseA

query
sites
8sseA

K

E

R

R

I

L

E

K

H

R

A

R

L

V

V

V

N

E

G

G

I

R

T

K

E

Q

F

G

I

L

E

E

A

A

D

D

T

L

E

E

A

A

R

L

L

K

E

A

A

G

E

H

R

K

P

P

L

L

H

D

V

L

I

I

E

N

G

G

P

P

L

L

M

L

A

A

G

G

M

M

N

K

V

E

V

V

G

G

D

D

L

L

F

F

G

G

A

A

G

G

K

K

M

M

F

Q

L

L

P

P

Q

F

V

V

V

L

K

Q

S

A

A

A

R

E

V

V

M

M

K

K

Q

R

A

A

V

V

A

A

V

Y

L

L

L

E

P

P

Y

H

M

M

E

E

A

-

E

-

K

-

L

-

A

-

N

-

G

-

S

-

G

-

Q

-

R

K

E

E

S

G

A

K

G

G

K

T

V

L

L

V

M

L

A

A

T

T

V

V

K

K

G

G

D

D

V

V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

L

V
|
V

G

D

V

I

L

L

A

S

C

N

N

N

N

G

Y

Y

E

R

I

V

D

N

L

L

G

G

V

I

M

K

V

V

P

P

S

I

A

E

K

E

I

I

L

L

Q

K

T

A

A

V

R

E

D

A

E

H

K

K

V

P

D

H

I

A

I

V

G

G

L

M

S
|
S

G

G

L

L

I
x
L

T
x
V

P

K

S

S

L

T

D

L

E

V

M

M

V

K

H

E

V

N

A

L

A

E

E

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M

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G

G

F

Y

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I

L

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P

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x
I

I

L

G
|
G

A
|
A

-

A

-

L

T

T

T

R

S

S

R

Y

V

V

H

E

T

E

A

L

V

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K

A

I

I

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Y

P

P

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N

Y

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H

Y

K

-

G

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Q

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Y

Y

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D

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L

L

L

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L

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Y

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D

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A

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L

L

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x
H

A

G

L

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x
V

R

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F

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T

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E

A

A

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L

A

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E

L

Y

M

W

H

H

Q

K

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W

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G

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A

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A

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Q

D

K

L

L

I

F

G

Q

E

Q

A

G

Y

Y

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Q

G

G

I

A

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P

Y

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x
S

P
x
F

G
|
G

Y
|
Y

P

P

A

A

Q

C

P

P

D

D

H

L

T

A

E

D

K
x
Q

E

A

T

K

L

L

F

D

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R

L

L

L

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D

G

A

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A

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K

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I

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G

V

V

R

R

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L

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F

F

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x
L

W

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P

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x
E

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H

S
x
A

V

T

S
|
S

G

A

I

L

Y

V

I

V

A

H

H

H

P

P

D

E

S

A

Y

R

Y

Y

F

F

G

S

V

V

binding cobalamin: H97 (= H784), G100 (= G787), V104 (= V791), S142 (= S829), L145 (≠ I832), V146 (≠ T833), I169 (≠ L856), G171 (= G858), G172 (= G859), A173 (= A860), H405 (≠ K1117), V409 (≠ D1121), S451 (≠ A1164), F452 (≠ P1165), G453 (= G1166), Y454 (= Y1167), Q463 (≠ K1176), L485 (≠ M1199), E488 (≠ G1202), A490 (≠ S1204), S492 (= S1206)

3bofA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima complexed with zn2+ and homocysteine (see paper)
27% identity, 47% coverage: 29:618/1262 of query aligns to 7:538/560 of 3bofA

query
sites
3bofA

L

V

T

S

K

K

A

L

R

S

E

E

R

R

I

V

L

L

I

L

L

L

D

D

G

G

A

A

M

Y

G

G

T

T

Q

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I

F

Q

M

G

K

L

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N

-

L

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F

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G

A

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D

I

V

L

I

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T

N

N

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F

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G

S

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|
C

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D

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F

F

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C

V

A

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P

P

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N

A

A

G

G

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K

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I

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N

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|
C

C

C

G

G

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H

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K

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G

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L

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N

S

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M

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K

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D

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H

K

A

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L

V

L

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A

K

Y

Y

G

G

A

G

A

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V

L

V

I

V

V

M

L

A

L

F

M

D

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D

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K

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V

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F

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D

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P

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G

V

V

F

L

A

P

V

L

A

G

T

A

G

-

I

-

E

-

H

-

N

E

G

G

Y

K

G

P

V

V

A

E

F

V

I

L

E

K

A

T

T

I

R

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F

I

I

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S

E

S

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K

L

-

P

-

H

G

V

F

H

N

I

T

S

T

G

V

G

G

I

L

S

S

N

N

L

L

S

S

F

F

S

G

F

L

R

-

G

-

N

-

E

-

P

P

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D

R

R

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S

A

Y

M

Y

H

N

A

T

V

A

F

F

L

L

Y

V

H

L

A

G

I

I

Q

S

V

K

G

G

M

L

D

S

M

S

G

A

I

I

V

M

N

N

binding zinc ion: C207 (= C267), C272 (= C330)

1q8jA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima (cd2+, hcy, methyltetrahydrofolate complex) (see paper)
27% identity, 47% coverage: 29:618/1262 of query aligns to 7:538/559 of 1q8jA

query
sites
1q8jA

L

V

T

S

K

K

A

L

R

S

E

E

R

R

I

V

L

L

I

L

L

L

D

D

G

G

A

A

M

Y

G

G

T

T

Q

E

I

F

Q

M

G

K

L

-

N

-

L

-

N

-

E

-

S

-

F

-

R

-

G

-

D

-

R

-

F

-

A

-

D

-

C

-

S

-

C

-

H

-

L

-

Q

Y

G

G

N

Y

N

D

D

D

L

L

-

P

-

E

L

L

I

N

L

I

T

K

Q

A

P

P

Q

D

A

V

I

V

E

L

D

K

I

V

H

H

Y

R

A

S

Y

Y

A

I

M

E

A

S

G

G

A

S

D

D

I

V

L

I

E

L

T

T

N

N

T

T

F

F

S

G

S

A

T

T

T

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I

M

A

K

Q

L

A

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D

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Y

H

S

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M

L

E

E

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D

V

K

V

L

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D

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P

L

I

N

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R

D

N

G

A

A

V

R

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L

I

A

A

R

R

A

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I

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K

-

A

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-

G

-

K

G

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E

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K

F

L

V

V

A

F

G

G

A

D

L

I

G

G

P

P

T

T

N

G

R

-

T

-

A

-

S

-

I

-

S

-

P

-

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E

V

L

N

P

N

Y

P

P

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L

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G

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K

A

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L

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D

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G

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L

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G

Q

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P

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A

A

N

F

F

W

A

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T

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D

H

E

A

L

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D

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I

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A

I

L

G

G

L

I

N

N

C

C

A

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L

L

G

G

A

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A

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M

I

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H

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D

D

A

K

F

F

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L

C

V

A

V

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E

P

P

N

N

A

A

G

G

L

K

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P

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I

-

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-

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N

N

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G

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A

A

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A

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L

G

G

L

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L

V

N

N

I

I

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F

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G

C

C

G

G

S

T

T

T

P

P

E

E

H

H

I

V

K

K

A

L

I

F

A

R

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A

V

V

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G

G

K

N

Y

R

G

K

P

P

R

L

E

Q

I

R

P

K

E

K

T

K

E

R

I

I

H

F

M

A

R

V

L

S

S

S

G

-

L

-

E

-

P

P

F

S

T

K

L

L

T

V

K

T

D

F

I

D

A

H

F

F

V

V

N

V

V

I

G

G

E
|
E

R

R

T

I

N

N

I

P

T

A

G

G

S

R

A

K

K

K

F

L

R

W

K

A

L

E

I

M

T

Q

S

K

G

G

D

N

F

E

A

E

T

I

A

V

L

I

D

K

V

E

A

A

R

K

D

T

Q

Q

V

V

A

E

N

K

G

G

A

A

Q

E

I

V

I

L

D

D

I

V

N

N

M

F

D

G

E

-

G

-

L

-

I

I

D

E

S

S

E

Q

K

I

A

D

M

V

V

R

E

Y

F

V

L

E

N

K

L

I

I

V

A

Q

A

T

E

L

P

P

D

Y

I

V

A

S

R

N

V

V

P

P

V

L

M

S

I

L

D
|
D

S

I

S

Q

K

N

W

V

D

D

V

L

I

T

E

E

A

R

G

A

L

L

K

R

C

A

V

Y

Q

P

G

G

K

R

P

S

L

L

V

F

N
|
N

S

S

I

A

S

K

M

V

K

D

E

E

G

E

E

E

Q

L

A

E

F

M

I

-

D

-

H

K

A

I

K

N

R

L

V

L

R

K

A

K

Y

Y

G

G

A

G

A

T

V

L

V

I

V

V

M

L

A

L

F

M

D

G

T

K

Q

D

G

V

Q

P

A

K

D

S

T

F

L

E

E

E

R

R

K

K

V

E

A

Y

I

F

S

E

T

-

R

K

A

A

Y

L

E

K

L

I

L

L

T

E

R

R

E

H

A

D

G

F

F

-

P

-

P

S

E

D

D

R

I

V

V

I

F

F

D
|
D

P

P

N

G

V

V

F

L

A

P

V

L

A

G

T

A

G

-

I

-

E

-

H

-

N

E

G

G

Y

K

G

P

V

V

A

E

F

V

I

L

E

K

A

T

T

I

R

E

Q

F

I

I

T

S

E

S

T

K

L

-

P

-

H

G

V

F

H

N

I

T

S

T

G

V

G
|
G

I

L

S

S

N
|
N

L

L

S

S

F
|
F

S

G

F

L

R

-

G

-

N

-

E

-

P

P

V

D

R
|
R

E

S

A

Y

M

Y

H

N

A

T

V

A

F

F

L

L

Y

V

H

L

A

G

I

I

Q

S

V

K

G

G

M

L

D

S

M

S

G

A

I
|
I

V

M

N

N

binding 5-methyl-5,6,7,8-tetrahydrofolic acid: E320 (= E381), D390 (= D454), N411 (= N475), D473 (= D542), G505 (= G581), N508 (= N584), F511 (= F587), R516 (= R596), I536 (= I616)

5vooA Methionine synthase folate-binding domain with methyltetrahydrofolate from thermus thermophilus hb8 (see paper)
34% identity, 22% coverage: 379:652/1262 of query aligns to 5:273/282 of 5vooA

query
sites
5vooA

V

V

G

G

E
|
E

R
|
R

T

L

N

N

I

A

T

T

G
|
G

S
|
S

A
x
K

K

R

F

F

R

R

K

E

L

M

I

L

T

F

S

A

G

R

D

D

F

L

A

E

T

G

A

I

L

L

D

A

V

L

A

A

R

R

D

E

Q

Q

V

V

A

E

N

E

G

G

A

A

Q

H

I

A

I

L

D

D

I

L

N

S

M

V

D

A

E

W

G

T

L

G

I

R

D

D

S

E

E

L

K

E

A

D

M

L

V

R

E

W

F

L

L

L

N

P

L

H

I

L

A

A

A

T

E

A

P

-

D

-

I

-

A

L

R

T

V

V

P

P

V

V

M

M

I

V

D
|
D

S

S

S

T

K

S

W

P

D

E

V

A

I

M

E

E

A

L

G

A

L

L

K

K

C

Y

V

L

Q

P

G

G

K

R

P

V

L

L

V

L

N
|
N

S

S

I

A

S

N

M

L

K

E

E

D

G

G

E

L

Q

E

A

R

F

F

I

D

D

R

H

V

A

A

K

S

R

L

V

A

R

K

A

A

Y

H

G

G

A

A

V

L

V

V

M

L

A

A

F

I

D

D

T

E

Q

K

G

G

Q

M

A

A

D

K

T

T

L

R

E

E

R

E

K

K

V

V

A

R

I

V

S

A

T

L

R

R

A

M

Y

Y

E

E

L

R

L

L

T

T

R

E

E

H

A

H

G

G

F

L

P

R

P

P

E

E

D

D

I

L

V

L

F

F

D
|
D

P

L

N

L

V

T

F

F

A

P

V

I

A

T

T

Q

G

G

I

D

E

E

H

S

N

R

G

P

Y

L

G

A

V

K

A

E

F

T

I

L

E

L

A

A

T

M

R

E

Q

E

I

L

T

R

E

E

T

R

L

L

P

P

H

G

V

V

H

G

I

F

S

V

G

L

G
|
G

I

V

S

S

N
|
N

L

V

S

S

F
|
F

S

G

F

L

R

K

G

P

N

R

E

-

P

-

V

A

R
|
R

E

R

A

V

M

L

H

N

A

S

V

V

F

F

L

L

Y

D

H

E

A

A

I

R

Q

K

V

R

G

G

M

L

D

T

M

A

G

A

I
|
I

V

V

N

D

A

A

G

G

Q

K

L

I

A

L

V

P

Y

I

E

S

S

Q

I

I

D

P

P

E

E

E

L

A

R

Y

E

A

A

L

C

A

E

L

D

D

V

L

V

I

L

Y

N

D

R

R

D

K

D

E

A

G

T

F

E

D

R

P

L

L

binding 5-methyl-5,6,7,8-tetrahydrofolic acid: E7 (= E381), R8 (= R382), G13 (= G387), S14 (= S388), K15 (≠ A389), D77 (= D454), N98 (= N475), D165 (= D542), G204 (= G581), N207 (= N584), F210 (= F587), R217 (= R596), I237 (= I616)

7xcnP Crystal structure of the mttb-mttc complex at 2.7 a resolution (see paper)
31% identity, 14% coverage: 690:864/1262 of query aligns to 14:188/215 of 7xcnP

query
sites
7xcnP

I

I

T

T

E

D

F

F

I

D

E

D

A

E

D

L

T

A

E

E

A

V

R

A

L

N

E

E

A

A

E

L

R

A

-

A

-

G

-

I

-

D

P

P

L

V

H

E

V

L

I

I

E

E

G

K

P

G

L

F

M

T

A

A

G

G

M

M

N

E

V

E

V

V

G

G

D

E

L

K

F

F

G

G

A

Q

G

G

K

E

M

L

F

F

L

L

P

P

Q

H

V

V

V

L

K

A

S

A

A

A

R

E

V

A

M

M

K

N

Q

S

A

G

V

I

A

K

V

V

L

I

L

T

P

P

Y

E

M

M

E

E

A

K

E

R

K

K

L

-

A

-

N

-

G

-

S

-

G

S

Q

Q

R

T

E

K

S

S

A

L

G

G

K

T

V

V

L

A

M

I

A

G

T

T

V

I

K

E

G

G

D
|
D

V
x
I

H
|
H

D

S

I
|
I

G
|
G

K

K

N

D

I

I

V
|
V

G

A

V

S

V

M

L

L

A

N

C

I

N

A

N

G

Y

F

E

K

I

V

D

D

L

L

G

G

V

R

M

D

V

V

P

P

S

I

A

N

K

T

I

F

L

V

Q

E

T

K

A

V

R

K

D

E

E

L

K

K

V

P

D

Q

I

V

G

A

L
x
S

S
|
S

G

A

L
|
L

I
x
M

T
|
T

P

T

S

T

L

M

D

V

E

N

M

Q

V

I

H

Q

V

I

A

E

E

Q

M

L

E

K

R

E

E

A

G

G

F

V

-

R

-

D

D

Q

I

V

P

K

L

T

L
x
M

I

V

G
|
G

A

A

T

P

T

V

S

T

R

Q

binding 5-hydroxybenzimidazolylcobamide: D104 (= D782), I105 (≠ V783), H106 (= H784), I108 (= I786), G109 (= G787), V113 (= V791), S150 (≠ L828), S151 (= S829), L153 (= L831), M154 (≠ I832), T155 (= T833), M180 (≠ L856), G182 (= G858), G183 (= G859)

Sites not aligning to the query:

binding 5-hydroxybenzimidazolylcobamide: 200, 202, 203

2i2xB Crystal structure of methanol:cobalamin methyltransferase complex mtabc from methanosarcina barkeri (see paper)
32% identity, 13% coverage: 700:864/1262 of query aligns to 60:216/258 of 2i2xB

query
sites
2i2xB

E

Q

A

A

R

A

L

I

E

E

A

A

E

G

R

K

-

D

P

P

L

I

H

D

V

L

I

I

E

D

G

D

P

A

L

L

M

M

A

V

G

G

M

M

N

G

V

V

G

I

D

R

L

L

F

Y

G

D

A

E

G

G

K

V

M

I

F

F

L

L

P

P

Q

N

V

V

V

M

K

M

S

S

A

A

R

D

V

A

M

M

K

L

Q

E

A

G

V

I

A

-

V

-

L

-

P

-

Y

-

M

-

E

-

A

-

E

E

K

Y

L

C

A

K

N

E

G

N

G

S

S

G

G

A

Q

T

R

P

E

K

S

T

A

K

G

G

K

T

V

V

L

V

M

C

A

H

T

V

V

A

K

E

G
|
G

D
|
D

V
|
V

H
|
H

D
|
D

I
|
I

G
|
G

K

K

N

N

I

I

V
|
V

G

T

V

A

V

L

L

L

A

R

C

A

N

N

N

G

Y

Y

E

N

I

V

D

D

L

L

G

G

V

R

M

D

V

V

P

P

S

A

A

E

K

E

I

V

L

L

Q

A

T

A

A

V

R

Q

D

K

E

E

K

K

V

P

D

I

I

M

I

L

G
x
T

L

G

S
x
T

G

A

L
|
L

I
x
M

T
|
T

P

T

S
x
T

L

M

D

Y

E

A

M

F

V

K

H

E

V

V

A

N

A

D

E

M

M

L

E

L

R

E

E

N

G

G

F

I

D

K

I

I

P

P

L

F

L
x
A

I

C

G
|
G

A
x
G

T

A

T

V

S

N

R

Q

active site: D134 (= D782), H136 (= H784), T187 (≠ S835)
binding 5-hydroxybenzimidazolylcob(iii)amide: G133 (= G781), D134 (= D782), V135 (= V783), H136 (= H784), D137 (= D785), I138 (= I786), G139 (= G787), V143 (= V791), T179 (≠ G827), T181 (≠ S829), L183 (= L831), M184 (≠ I832), T185 (= T833), A208 (≠ L856), G210 (= G858), G211 (= G859), G212 (≠ A860)

Sites not aligning to the query:

binding 5-hydroxybenzimidazolylcob(iii)amide: 228, 229, 230, 231

Q46EH4 Methanol--corrinoid protein; Methanol:corrinoid methyltransferase 1 subunit of 27 kDa; MT1 subunit 27 kDa from Methanosarcina barkeri (strain Fusaro / DSM 804) (see paper)
32% identity, 13% coverage: 700:864/1262 of query aligns to 60:216/258 of Q46EH4

query
sites
Q46EH4

E

Q

A

A

R

A

L

I

E

E

A

A

E

G

R

K

-

D

P

P

L

I

H

D

V

L

I

I

E

D

G

D

P

A

L

L

M

M

A

V

G

G

M

M

N

G

V

V

G

I

D

R

L

L

F

Y

G

D

A

E

G

G

K

V

M

I

F

F

L

L

P

P

Q

N

V

V

V

M

K

M

S

S

A

A

R

D

V

A

M

M

K

L

Q

E

A

G

V

I

A

-

V

-

L

-

P

-

Y

-

M

-

E

-

A

-

E

E

K

Y

L

C

A

K

N

E

G

N

G

S

S

G

G

A

Q

T

R

P

E

K

S

T

A

K

G

G

K

T

V

V

L

V

M

C

A
x
H

T

V

V

A

K

E

G

G

D

D

V

V

H
|
H

D

D

I

I

G

G

K

K

N

N

I

I

V

V

G

T

V

A

V

L

L

L

A

R

C

A

N

N

N

G

Y

Y

E

N

I

V

D

D

L

L

G

G

V

R

M

D

V

V

P

P

S

A

A

E

K

E

I

V

L

L

Q

A

T

A

A

V

R

Q

D

K

E

E

K

K

V

P

D

I

I

M

I

L

G

T

L

G

S

T

G

A

L

L

I

M

T

T

P

T

S

T

L

M

D

Y

E

A

M

F

V

K

H

E

V

V

A

N

A

D

E

M

M

L

E

L

R

E

E

N

G

G

F

I

D

K

I

I

P

P

L

F

L

A

I

C

G

G

A

G

T

A

T

V

S

N

R

Q

H129 (≠ A777) mutation to K: Does not affect cobalamin-binding.
H136 (= H784) mutation H->G,K: Abolishes cobalamin-binding.

Sites not aligning to the query:

256:258 HKH→KKK: Does not affect cobalamin-binding.

4jgiB 1.5 angstrom crystal structure of a novel cobalamin-binding protein from desulfitobacterium hafniense dcb-2 (see paper)
32% identity, 11% coverage: 719:859/1262 of query aligns to 45:174/206 of 4jgiB

query
sites
4jgiB

G

G

M

M

N

T

V

K

V

V

G

G

D

E

L

R

F

F

G

D

A

S

G

G

K

E

M

Y

F

F

L

I

P

G

Q

D

V
x
L

V

I

K

F

S

A

A

G

R

E

V

I

M

L

K

Q

Q

A

A

A

V

M

A

D

V

K

L

L

L

K

P

P

Y

A

M

L

E

E

A

K

E

R

K

-

L

-

A

-

N

-

G

-

S

-

G

-

Q

-

R

-

E

-

S

-

A

-

G

A

K

K

V

I

L

V

M

L

A

A

T

T

V

V

K

E

G

G

D
|
D

V
x
L

H
|
H

D
|
D

I
|
I

G
|
G

K

K

N

N

I

I

V
x
F

G

R

V

T

V

M

L

A

A

E

C

A

N

S

N

G

Y

F

E

E

I

V

I

F

D

D

L

L

G

G

V

I

M

D

V

V

P

P

S

V

A

K

K

I

I

I

L

V

Q

D

T

K

A

V

R

K

D

E

E

V

K

N

V

P

D

E

I

I

I

V

G
|
G

L

L

S
|
S

G

G

L

V

I
x
L

T

T

P

L

S
x
A

L

L

D

D

E

S

M

M

V

R

H

E

V

T

A

V

A

D

E

A

M

L

E

K

R

A

E

E

G

G

F

L

-

R

-

N

D

D

I

L

P

K

L

V

L

I

I

I

G
|
G

active site: D95 (= D782), H97 (= H784), A148 (≠ S835)
binding co-methylcobalamin: L63 (≠ V737), D95 (= D782), L96 (≠ V783), H97 (= H784), D98 (= D785), I99 (= I786), G100 (= G787), F104 (≠ V791), G140 (= G827), S142 (= S829), L145 (≠ I832), G173 (= G858), G174 (= G859)

Sites not aligning to the query:

binding co-methylcobalamin: 175, 191, 192, 193, 194

3ezxA Structure of methanosarcina barkeri monomethylamine corrinoid protein
35% identity, 12% coverage: 709:863/1262 of query aligns to 36:185/212 of 3ezxA

query
sites
3ezxA

L

L

H

D

V

I

I

I

E

T

G

K

P

G

L

L

M

S

A

V

G

G

M
|
M

N

K

V

I

V

V

G

G

D

D

L

K

F
|
F

G

E

A

A

G

A

K

E

M

I

F

F

L

L

P

P

Q

Q

V

I

V

M

K

M

S

S

A

G

R

K

V

A

M

M

K

S

Q

N

A

A

V

M

A

E

V

V

L

L

L

T

P

P

Y

E

M

L

E

E

A

K

E

N

K

K

L

-

A

-

N

-

G

-

S

-

G

-

Q

-

R

-

E

K

S

E

A

A

G

G

K

L

V

A

L

I

M

T

A

F

T

V

V

A

K

E

G

G

D
|
D

V
x
I

H
|
H

D
|
D

I
|
I

G

G

K

H

N

R

I

L

V
|
V

G

T

V

T

V

M

L

L

A

G

C

A

N

N

N

G

Y

F

E

Q

I

I

I

V

D

D

L

L

G

G

V

V

M

D

V

V

P

L

S

N

A

E

K

N

I

V

L

V

Q

E

T

E

A

A

-

A

-

K

-

H

R

K

D

G

E

E

K

K

V

V

D

L

I

L

I
x
V

G

G

L

-

S
|
S

G

A

L
|
L

I
x
M

T
|
T

P

T

S
|
S

L

M

-

L

-

G

-

Q

-

K

D

D

E

L

M

M

V

D

H

R

V

L

A

N

A

E

E

E

M

K

E

L

R

R

E

D

G

-

F

-

D

S

I

V

P

K

L

C

L
x
M

I

F

G
|
G

A

A

T

P

T

V

S

S

active site: D100 (= D782), H102 (= H784), S155 (= S835)
binding 5-hydroxybenzimidazolylcobamide: M47 (= M720), F54 (= F727), D100 (= D782), I101 (≠ V783), H102 (= H784), D103 (= D785), I104 (= I786), V109 (= V791), V147 (≠ I826), S149 (= S829), L151 (= L831), M152 (≠ I832), T153 (= T833), M178 (≠ L856), G180 (= G858), G181 (= G859)

Sites not aligning to the query:

binding 5-hydroxybenzimidazolylcobamide: 198, 200

4o1eA Structure of a methyltransferase component in complex with mthf involved in o-demethylation (see paper)
27% identity, 19% coverage: 376:618/1262 of query aligns to 5:232/271 of 4o1eA

query
sites
4o1eA

F

L

V

I

N

I

V

I

G

G

E
|
E

R
x
K

T

I

N

N

I

G

T

T

G
x
I

S

P

A

S

K

-

F

V

R

K

K

K

L

A

I

I

T

E

S

A

G

K

D

D

F

E

A

K

T

L

A

I

L

R

D

D

V

L

A

A

R

L

D

R

Q

Q

V

S

A

E

N

A

G

G

A

A

Q

D

I

Y

I

I

D

D

I

V

N

C

M

A

D

S

E

T

G

-

L

-

I

-

D

-

S

S

E

P

K

E

A

L

M

E

V

V

E

E

F

T

L

L

N

Q

-

W

L

L

I

M

A

D

A

I

E

V

P

Q

D

E

I

A

A

T

R

D

V

T

P

P

V

L

M

C

I

I

D

D

S

S

S

P

K

N

W

P

D

R

V

A

I

I

E

Q

A

Q

G

V

L

L

K

L

C

Y

V

A

Q

K

G

R

K

P

P

G

L

L

V

I

N
|
N

S

S

I

V

S

S

M

L

K

-

E

E

G

G

E

D

Q

K

A

C

F

E

I

V

D

I

H

F

A

P

K

-

R

L

V

I

R

Q

A

G

Y

T

G

S

A

W

A

Q

V

V

V

I

V

A

M

L

A

T

F

C

D

D

T

N

Q

S

G

G

Q

I

A

P

D

Q

T

D

L

V

E

Q

R

S

K

R

V

V

A

E

I

I

S

A

T

Q

R

A

A

L

Y

V

E

E

L

K

L

-

T

A

R

Q

E

S

A

Y

G

D

F

I

P

A

P

Q

E

E

D

R

I

I

V

H

F

I

D
|
D

P

P

N

L

V

V

F

I

A

A

V

L

A

S

T

A

G

-

I

-

E

-

H

D

N

N

G

G

Y

A

G

L

V

L

A

K

F

F

I

A

E

E

A

A

T

T

R

R

Q

Q

I

I

T

K

E

A

T

N

L

Y

P

P

H

M

V

I

H

N

I

V

S

T

G

S

G

G

I

L

S

S

N

N

L

I

S

S

F

F

S

G

F

M

R

-

G

-

N

-

E

-

P

P

V

L

R

R

E
x
K

A

V

M

V

H

N

A

Q

V

N

F

F

L

L

Y

T

H

L

A

A

I

M

Q

F

V

A

G

G

M

M

D

D

M

S

G

A

I
|
I

V

L

N
x
D

binding 5-methyl-5,6,7,8-tetrahydrofolic acid: E10 (= E381), K11 (≠ R382), I16 (≠ G387), N100 (= N475), D164 (= D542), K211 (≠ E597), I230 (= I616), D232 (≠ N618)

Sites not aligning to the query:

binding 5-methyl-5,6,7,8-tetrahydrofolic acid: 235

Query Sequence

>WP_106713177.1 NCBI__GCF_003010955.1:WP_106713177.1
MNIAVDSLGENLFGPMGSPRDGSEVFAALTKAARERILILDGAMGTQIQGLNLNEESFRG
DRFADCSCHLQGNNDLLILTQPQAIEDIHYAYAMAGADILETNTFSSTTIAQADYSMEEV
VYELNRDGARLARRAAIKAEQKDGKRRFVAGALGPTNRTASISPDVNNPGYRAVTFDDLR
IAYAEQVRGLIDGGADIILIETIFDTLNAKAAIFATQEVFEEKGITLPIMISGTITDRSG
RTLSGQTPTAFWYSVRHAKPFTIGLNCALGANAMRDHLAEIASVADAFVCAYPNAGLPNE
FGQYDESPEAMAAQLEEFAREGLLNIVGGCCGSTPEHIKAIAEAVGKYGPREIPETEIHM
RLSGLEPFTLTKDIAFVNVGERTNITGSAKFRKLITSGDFATALDVARDQVANGAQIIDI
NMDEGLIDSEKAMVEFLNLIAAEPDIARVPVMIDSSKWDVIEAGLKCVQGKPLVNSISMK
EGEQAFIDHAKRVRAYGAAVVVMAFDTQGQADTLERKVAISTRAYELLTREAGFPPEDIV
FDPNVFAVATGIEEHNGYGVAFIEATRQITETLPHVHISGGISNLSFSFRGNEPVREAMH
AVFLYHAIQVGMDMGIVNAGQLAVYESIDPELREACEDVVLNRRDDATERLLDLAERYKG
AAGKEARERDLAWREWSVEKRIEHALVNGITEFIEADTEEARLEAERPLHVIEGPLMAGM
NVVGDLFGAGKMFLPQVVKSARVMKQAVAVLLPYMEAEKLANGGSGQRESAGKVLMATVK
GDVHDIGKNIVGVVLACNNYEIIDLGVMVPSAKILQTARDEKVDIIGLSGLITPSLDEMV
HVAAEMEREGFDIPLLIGGATTSRVHTAVKINPRYHKGQTVYVTDASRAVGVVSNLLSLE
AKPGYVDGVRAEYTKVAEAHARNEADKQRLSLAKARANAQMVDWVSYTPPKPSFLGTRVF
ENYDLAEIARYIDWTPFFQTWELKGRYPAILEDEKQGPAARQLFDDAQAMLKKIIAEKWF
NPKAVIGLWPAGAVGDDIRLFTDEARGQELATFFTLRQQLSKRDGRPNVALSDFVAPVDS
GKEDYVGGFVVTAGIEEVAIAERFERANDDYSSILVKALADRFAEAFAELMHQRVRKEFW
GYAPEENLASDDLIGEAYRGIRPAPGYPAQPDHTEKETLFRLLDAEAKIDVRLTESFAMW
PGSSVSGIYIAHPDSYYFGVAKVERDQVEDYAARKSMPVADVERWLGPILNYVPAPRVQA
AE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory