SitesBLAST
Comparing WP_106713194.1 NCBI__GCF_003010955.1:WP_106713194.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
60% identity, 100% coverage: 1:258/259 of query aligns to 2:259/260 of 2ztlA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), L200 (≠ Q199)
- binding (3s)-3-hydroxybutanoic acid: Q94 (= Q93), S142 (= S141), H144 (= H143), K152 (= K151), Y155 (= Y154), Q196 (= Q195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), G15 (= G14), I16 (= I15), F36 (= F35), L64 (≠ M63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I112), Y155 (= Y154), K159 (= K158), P185 (= P184), W187 (≠ Y186), V188 (= V187), T190 (= T189), V193 (= V192)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
60% identity, 100% coverage: 1:258/259 of query aligns to 2:259/260 of 1wmbA
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
59% identity, 98% coverage: 4:258/259 of query aligns to 5:259/260 of 5b4tA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q93), S142 (= S141), H144 (= H143), K152 (= K151), Y155 (= Y154), W187 (≠ Y186), Q196 (= Q195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ N12), G15 (= G14), I16 (= I15), F36 (= F35), D63 (= D62), L64 (≠ M63), N90 (= N89), G92 (= G91), L113 (≠ I112), I140 (= I139), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ Y186), V188 (= V187), T190 (= T189), L192 (= L191), V193 (= V192)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
59% identity, 98% coverage: 4:258/259 of query aligns to 5:259/260 of 3w8dA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding methylmalonic acid: Q94 (= Q93), S142 (= S141), H144 (= H143), K152 (= K151), Y155 (= Y154), W187 (≠ Y186), Q196 (= Q195), W257 (= W256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ N12), S14 (= S13), G15 (= G14), I16 (= I15), F36 (= F35), A62 (= A61), D63 (= D62), L64 (≠ M63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I112), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ Y186), V188 (= V187), T190 (= T189), L192 (= L191), V193 (= V192)
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
59% identity, 98% coverage: 4:258/259 of query aligns to 5:259/260 of 3vdrA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q93), H144 (= H143), K152 (= K151), Y155 (= Y154), W187 (≠ Y186), Q196 (= Q195), W257 (= W256)
- binding acetoacetic acid: Q94 (= Q93), H144 (= H143), K152 (= K151), Y155 (= Y154), W187 (≠ Y186), Q196 (= Q195), W257 (= W256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ N12), I16 (= I15), F36 (= F35), D63 (= D62), L64 (≠ M63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I112), K159 (= K158), G186 (= G185), V188 (= V187), T190 (= T189), L192 (= L191), V193 (= V192)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G10), T13 (≠ N12), I16 (= I15), F36 (= F35), D63 (= D62), L64 (≠ M63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I112), S142 (= S141), Y155 (= Y154), K159 (= K158), G186 (= G185), V188 (= V187), T190 (= T189), L192 (= L191), V193 (= V192)
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
59% identity, 98% coverage: 4:258/259 of query aligns to 5:259/260 of 3vdqA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding acetate ion: Q94 (= Q93), H144 (= H143), K152 (= K151), W187 (≠ Y186), L192 (= L191), Q196 (= Q195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), S14 (= S13), I16 (= I15), F36 (= F35), D63 (= D62), L64 (≠ M63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I112), I140 (= I139), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ Y186), V188 (= V187), T190 (= T189), L192 (= L191), V193 (= V192)
1x1tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas fragi complexed with NAD+ (see paper)
56% identity, 100% coverage: 1:258/259 of query aligns to 2:235/236 of 1x1tA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158)
- binding cacodylate ion: S142 (= S141), H144 (= H143), Y155 (= Y154), W187 (≠ Y186), W233 (= W256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ N12), S14 (= S13), G15 (= G14), I16 (= I15), G35 (≠ S34), F36 (= F35), D63 (= D62), L64 (≠ M63), N90 (= N89), G92 (= G91), L113 (≠ I112), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), W187 (≠ Y186), V188 (= V187), T190 (≠ E213)
5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
52% identity, 100% coverage: 1:258/259 of query aligns to 2:254/255 of 5yssB
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ N12), S14 (= S13), G15 (= G14), I16 (= I15), G35 (≠ S34), F36 (= F35), L60 (≠ M63), N86 (= N89), G88 (= G91), I89 (= I92), A137 (= A140), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), V184 (= V187), T186 (= T189)
2q2qD Structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas putida (see paper)
53% identity, 100% coverage: 1:258/259 of query aligns to 2:254/255 of 2q2qD
- active site: G15 (= G14), S138 (= S141), Y151 (= Y154), K155 (= K158), R196 (≠ Q199)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ N12), S14 (= S13), G15 (= G14), I16 (= I15), F36 (= F35), D59 (= D62), L60 (≠ M63), N86 (= N89), G88 (= G91), L109 (≠ I112), I136 (= I139), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), W183 (≠ Y186), V184 (= V187), T186 (= T189), L188 (= L191), V189 (= V192)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
41% identity, 98% coverage: 4:258/259 of query aligns to 8:260/261 of 6zzsD
- active site: G18 (= G14), S143 (= S141), Y156 (= Y154)
- binding nicotinamide-adenine-dinucleotide: G14 (= G10), S17 (= S13), I19 (= I15), D38 (≠ S34), M39 (≠ F35), D64 (= D62), V65 (≠ M63), N91 (= N89), A92 (= A90), G93 (= G91), M141 (≠ I139), A142 (= A140), S143 (= S141), Y156 (= Y154), K160 (= K158), P186 (= P184), G187 (= G185), V189 (= V187), T191 (= T189), L193 (= L191)
- binding 3-oxidanylidenepentanoic acid: Q95 (= Q93), S143 (= S141), N145 (≠ H143), K153 (= K151), Y156 (= Y154), Q197 (= Q195)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
41% identity, 98% coverage: 4:258/259 of query aligns to 7:259/260 of 6zzqA
- active site: G17 (= G14), S142 (= S141), Y155 (= Y154)
- binding acetoacetic acid: Q94 (= Q93), S142 (= S141), K152 (= K151), Y155 (= Y154), Q196 (= Q195)
- binding nicotinamide-adenine-dinucleotide: G13 (= G10), S16 (= S13), G17 (= G14), I18 (= I15), D37 (≠ S34), M38 (≠ F35), D63 (= D62), V64 (≠ M63), N90 (= N89), A91 (= A90), G92 (= G91), M140 (≠ I139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), Y187 (= Y186), V188 (= V187), T190 (= T189)
8dt1C Crystal structure of a putative d-beta-hydroxybutyrate dehydrogenase from burkholderia cenocepacia j2315 in complex with NAD
43% identity, 98% coverage: 4:256/259 of query aligns to 5:256/259 of 8dt1C
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), S14 (= S13), G15 (= G14), I16 (= I15), D35 (= D37), L36 (≠ R38), M60 (≠ A61), V62 (≠ M63), N88 (= N89), A89 (= A90), M139 (≠ I139), Y154 (= Y154), K158 (= K158), P184 (= P184), G185 (= G185), F186 (≠ Y186), V187 (= V187), T189 (= T189)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
37% identity, 100% coverage: 1:258/259 of query aligns to 3:247/248 of 6ixmC
- active site: G16 (= G14), S142 (= S141), Y155 (= Y154), K159 (= K158)
- binding nicotinamide-adenine-dinucleotide: G12 (= G10), S15 (= S13), G16 (= G14), I17 (= I15), D36 (= D37), I37 (≠ R38), A61 (= A61), D62 (= D62), T63 (≠ M63), N89 (= N89), A90 (= A90), M140 (≠ I139), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), A186 (≠ G185), Y187 (= Y186), I188 (≠ V187), L192 (= L191)
6zzpA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and 3-oxovalerate (see paper)
39% identity, 99% coverage: 1:256/259 of query aligns to 8:262/265 of 6zzpA
- binding nicotinamide-adenine-dinucleotide: G17 (= G10), S20 (= S13), G21 (= G14), I22 (= I15), D41 (≠ S34), I42 (≠ F35), M66 (≠ A61), D67 (= D62), V68 (≠ M63), N94 (= N89), A95 (= A90), G96 (= G91), M145 (≠ I139), S147 (= S141), Y160 (= Y154), K164 (= K158), P190 (= P184), F192 (≠ Y186), V193 (= V187), T195 (= T189), L197 (= L191), V198 (= V192)
- binding 3-oxidanylidenepentanoic acid: Q98 (= Q93), S147 (= S141), H149 (= H143), K157 (= K151), Y160 (= Y154), F192 (≠ Y186), Q201 (= Q195)
6zzoC Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and acetoacetate (see paper)
39% identity, 99% coverage: 1:256/259 of query aligns to 8:262/265 of 6zzoC
- binding acetoacetic acid: Q98 (= Q93), H149 (= H143), K157 (= K151), F192 (≠ Y186), Q201 (= Q195)
- binding nicotinamide-adenine-dinucleotide: G17 (= G10), S20 (= S13), G21 (= G14), I22 (= I15), D41 (≠ S34), I42 (≠ F35), M66 (≠ A61), D67 (= D62), V68 (≠ M63), N94 (= N89), A95 (= A90), G96 (= G91), M145 (≠ I139), Y160 (= Y154), K164 (= K158), P190 (= P184), F192 (≠ Y186), V193 (= V187), T195 (= T189), L197 (= L191), V198 (= V192)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
35% identity, 100% coverage: 1:258/259 of query aligns to 5:254/255 of 5itvA