SitesBLAST

V174 (≠ A152) binding FAD
H244 (≠ R226) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (≠ M285) binding substrate
327:333 (vs. 308:314, 14% identical) binding FAD
R329 (≠ G310) binding substrate

Sites not aligning to the query:

14:15 binding FAD
34:35 binding FAD
42:43 binding FAD
47:49 binding FAD
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
21% identity, 79% coverage: 69:327/328 of query aligns to 86:344/363 of 7cyxA

query
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7cyxA

G

G

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Y

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binding flavin-adenine dinucleotide: V171 (≠ A152), G200 (≠ S180), G201 (= G181), W203 (≠ Y183), G298 (= G283), R300 (≠ M285), P301 (≠ S286), Y326 (≠ G309), R327 (≠ G310), N328 (≠ H311), G329 (= G312), I330 (≠ L313)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 8, 10, 11, 12, 30, 31, 32, 38, 39, 40, 43, 45, 46

Query Sequence

>WP_106714347.1 NCBI__GCF_003010955.1:WP_106714347.1
LEAERIGWGASGRNGGFVSPGYANDGDAIERAVGADHARKLHALSIEGMKFVRDTIDELD
IVAAAPTHGIMSVLRYENSQALLARADYLQREFDYSVEYMSRDQVQNVLRSKRYFQALRD
PQAFHIHPLNYLRSAAREIRRLGGKIFEGSPALSCDFSRSEKIVRTARGEIRTRRVIVAS
GGYTTGLIPKMKRSFLPIATYVMMTEEAPDLIRQAITTTDAVGDNRRAGDYYRVIEGGKR
LLWGGRITTRAASPAALAVELHREMARTYPQLADLKIETSWSGLMSYARHLMPQIGQLHS
DVWYCTAFGGHGLNTTAIGGKVIAEAIL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory