SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_106716016.1 NCBI__GCF_003010935.1:WP_106716016.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WGB5 O-succinylhomoserine sulfhydrylase; OSH sulfhydrylase; OSHS sulfhydrylase; EC 2.5.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
51% identity, 96% coverage: 12:394/397 of query aligns to 20:406/406 of P9WGB5

query
sites
P9WGB5

A

A

T

T

Q

V

L

G

V

V

H

R

A

G

G

G

T

M

L

L

R

R

S

S

G

G

F

F

G

E

E

E

T

T

S

A

E

E

A

A

L

M

F

Y

L

L

T

T

Q

S

G

G

F

Y

V

V

Y

Y

P

G

S

S

A

A

E

A

A

V

A

A

E

E

A

K

R

S

F

F

K

A

G

G

E

E

E

L

P

D

G

H

F

Y

I

V

Y

Y

S

S

R

R

Y

Y

A

G

N

N

P

P

T

T

T

V

D

S

M

V

F

F

E

E

K

E

R

R

M

L

C

R

A

L

L

I

E

E

G

G

A

A

E

P

D

A

A

A

R

F

A

A

T

T

A

A

S

S

G

G

M

M

A

A

V

V

A

F

A

T

A

S

I

L

I

G

C

A

Q

L

V

L

K

G

S

A

G

G

D

D

H

R

V

L

L

V

A

A

R

R

A

S

M

L

F

F

G

G

S

S

C

C

R

F

Y

V

I

V

I

C

E

S

T

E

L

I

L

L

P

P

R

R

Y

W

G

G

V

V

E

Q

F

T

T

V

L

F

I

V

D

D

G

G

S

D

K

D

I

L

E

S

N

Q

W

W

Q

E

K

R

A

A

V

L

R

S

P

V

N

P

T

T

K

Q

V

A

M

V

F

F

L

F

E

E

S

T

P

P

T

S

N

N

P

P

T

M

L

Q

E

S

V

L

I

V

D

D

I

I

A

A

V

V

A

T

N

E

I

L

A

A

N

H

S

A

V

A

G

G

A

A

K

K

L

V

I

V

V

L

D

D

N

N

V

V

F

F

A

A

T

T

P

P

L

L

F

L

Q

Q

K

Q

P

G

L

F

E

P

L

L

G

G

A

V

H

D

I

V

V

V

Y

Y

S

S

T

G

T

T

K
|
K

H

H

I

I

D

D

G

G

Q

Q

G

G

R

R

C

V

L

L

G

G

V

A

I

I

L

L

S

G

D

D

K

R

Q

E

W

Y

V

I

D

D

E

G

N

P

L

V

H

Q

E

K

Y

L

F

M

R

R

H

H

T

T

G

G

P

P

G

A

L

M

S

S

P

A

F

F

N

N

S

A

W

W

V

V

M

L

L

L

K

K

G

G

M

L

E

E

T

T

L

L

V

A

V

I

R

R

V

V

R

Q

Q

H

Q

S

T

N

E

A

S

S

A

A

G

Q

K

R

I

I

A

A

D

E

F

F

L

L

A

N

D

G

H

H

P

P

A

S

V

V

S

R

K

W

V

V

I

R

Y

Y

P

P

G

Y

R

L

P

P

D

S

H

H

P

P

Q

Q

A

Y

D

D

I

L

I

A

A

K

R

R

Q

Q

M

M

S

S

G

G

S

G

T

T

L

V

I

V

A

T

F

F

E

A

V

L

K

D

G

C

-

P

-

E

-

D

-

V

G

A

K

K

K

Q

A

R

T

A

F

F

N

E

A

V

E

L

N

D

K

K

L

M

E

R

I

L

I

I

Q

D

I

I

S

S

N

N

L

L

G

G

D

D

S

A

K

K

S

S

L

L

I

V

T

T

H

H

P

P

T

A

T

T

H

H

K

R

N

A

L

M

T

G

D

P

D

E

A

G

R

R

A

A

E

A

L

I

G

G

I

L

S

G

D

D

G

G

L

V

R

R

L

I

S

S

V

V

G

G

L

L

E

E

D

D

T

T

D

D

L

L

I

I

E

A

D

D

L

I

D

D

V

R

A

A

L

L

K

S

K219 (= K211) modified: N6-(pyridoxal phosphate)lysine

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
43% identity, 97% coverage: 13:396/397 of query aligns to 10:395/396 of 4omaA

query
sites
4omaA

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

P

S

G

G

F

Y

I

I

Y

Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

S

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

T

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

active site: R59 (= R61), Y112 (≠ F114), D184 (= D186), K209 (= K211)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G89), I88 (≠ M90), Y112 (≠ F114), D184 (= D186), S206 (= S208), T208 (= T210), K209 (= K211), V337 (≠ N338), S338 (≠ N339), R373 (= R374)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
43% identity, 97% coverage: 13:396/397 of query aligns to 10:395/396 of 3jwbA

query
sites
3jwbA

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

P

S

G

G

F

Y

I

I

Y

Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G

G

M

I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

S

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S

S

T

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N

V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

active site: R59 (= R61), Y112 (≠ F114), D184 (= D186), K209 (= K211)
binding norleucine: Y112 (≠ F114), K209 (= K211), S338 (≠ N339), R373 (= R374)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
43% identity, 97% coverage: 13:396/397 of query aligns to 10:395/396 of 3jwaA

query
sites
3jwaA

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

P

S

G

G

F

Y

I

I

Y

Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G

G

M

I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

S

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S

S

T

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

active site: R59 (= R61), Y112 (≠ F114), D184 (= D186), K209 (= K211)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (≠ F114), K209 (= K211), V337 (≠ N338), S338 (≠ N339), R373 (= R374)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
43% identity, 97% coverage: 13:396/397 of query aligns to 10:395/396 of 3jw9A

query
sites
3jw9A

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

P

S

G

G

F

Y

I

I

Y

Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G

G

M

I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

S

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S

S

T

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

active site: R59 (= R61), Y112 (≠ F114), D184 (= D186), K209 (= K211)
binding S-ethyl-L-cysteine: Y112 (≠ F114), K209 (= K211), V337 (≠ N338), S338 (≠ N339)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
43% identity, 97% coverage: 13:396/397 of query aligns to 9:394/395 of 5m3zA

query
sites
5m3zA

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

P

S

G

G

F

Y

I

I

Y

Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S
x
H

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

S

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V
x
T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

T

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

active site: R58 (= R61), Y111 (≠ F114), D183 (= D186), K208 (= K211)
binding norleucine: Y111 (≠ F114), H113 (≠ S116), K208 (= K211), V336 (≠ N338), S337 (≠ N339)
binding pyridoxal-5'-phosphate: G86 (= G89), I87 (≠ M90), Y111 (≠ F114), E154 (= E157), D183 (= D186), T185 (≠ V188), S205 (= S208), T207 (= T210), K208 (= K211)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G89), I87 (≠ M90), Y111 (≠ F114), D183 (= D186), S205 (= S208), T207 (= T210), K208 (= K211), V336 (≠ N338), S337 (≠ N339), R372 (= R374)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
43% identity, 97% coverage: 13:396/397 of query aligns to 10:395/396 of 6egrA

query
sites
6egrA

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

P

S

G

G

F

Y

I

I

Y

Y

S

T

R

R

Y

L

A

G

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

S

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V
x
T

F
|
F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

T

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N

V

N

S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

Y

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

binding pyridoxal-5'-phosphate: G87 (= G89), I88 (≠ M90), Y112 (≠ F114), E155 (= E157), D184 (= D186), T186 (≠ V188), F187 (= F189), S206 (= S208), T208 (= T210), K209 (= K211)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
43% identity, 97% coverage: 13:396/397 of query aligns to 10:395/396 of 4hf8A

query
sites
4hf8A

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

P

S

G

G

F

Y

I

I

Y

Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

R

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N
|
N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

T

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N

V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

active site: R59 (= R61), Y112 (≠ F114), D184 (= D186), K209 (= K211)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G89), I88 (≠ M90), Y112 (≠ F114), E155 (= E157), N159 (= N161), D184 (= D186), S206 (= S208), K209 (= K211), S338 (≠ N339), R373 (= R374)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
41% identity, 97% coverage: 13:396/397 of query aligns to 10:384/386 of 3mkjA

query
sites
3mkjA

T

T

Q

Q

L

I

V

V

H

H

A

A

G

G

T

Q

L

Q

R

P

S

D

-

P

G

S

F

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

L

G

G

E

-

P

-

G

-

F

-

I

-

Y

-

S

-

R

-

Y

-

A

-

N

N

P

P

T

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

A

T

I

L

C

T

Q

L

V

C

K

Q

S

Q

G

G

D

D

H

H

V

I

L

V

A

S

A

A

R

S

A

A

M

I

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

F

I

L

E

S

T

H

L

S

L

M

P

P

R

K

Y

F

G

G

V

I

E

N

F

V

T

S

L

F

I

V

D

D

G

A

S

A

K

K

I

P

E

E

N

E

W

I

Q

R

K

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

M

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

N

G

I

I

A

A

N

H

S

Q

V

Q

G

G

A

A

K

L

L

L

I

V

V

V

D
|
D

N

N

V

T

F
|
F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

T

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

D

K

K

Q

Q

E

W

F

V

I

D

D

E

Q

N

A

L

R

H

F

E

V

Y

G

F

L

R

K

H

D

-

I

T

T

G

G

P

G

G

C

L

M

S

S

P

P

F

F

N

N

S

A

W

W

V

L

M

T

L

L

K

R

G

G

M

V

E

K

T

T

L

L

V

G

V

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

G

L

K

K

I

I

A

A

D

R

F

F

L

L

A

E

D

G

H

H

P

P

A

S

V

I

S

T

K

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

R

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

I

I

A

S

F

F

E

E

V

I

K

A

G

G

K

L

K

E

A

A

T

G

F

R

N

R

A

M

E

I

N

N

K

S

L

V

E

E

I

L

I

C

Q

L

I

L

S

A

N

V

N

S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

D

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

P

D

E

D

D

L

I

I

I

E

N

D

D

L

L

D

E

V

H

A

A

L

I

K

R

G

K

A

A

active site: Y101 (≠ F114), D173 (= D186), K198 (= K211)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G89), I77 (≠ M90), Y101 (≠ F114), E144 (= E157), D173 (= D186), F176 (= F189), S195 (= S208), T197 (= T210), K198 (= K211)

5dx5A Crystal structure of methionine gamma-lyase from clostridium sporogenes (see paper)
40% identity, 97% coverage: 7:393/397 of query aligns to 4:394/399 of 5dx5A

query
sites
5dx5A

R

K

K

K

L

M

R

G

P

F

A

A

T

T

Q

K

L

A

V

I

H

H

A

G

G

G

T

H

L

I

-

G

R

D

S

K

G

Q

F

F

G

G

E

S

T

L

S

A

E

T

A

P

L

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

I

Y

F

P

D

S

S

A

A

E

E

A

Q

A

G

E

G

A

R

R

R

F

F

K

A

G

G

E

E

P

S

G

G

F

Y

I

I

Y
|
Y

S

S

R
|
R

Y

L

A

G

N

N

P

P

T

T

T

S

D

T

M

E

F

V

E

E

K

N

R

K

M

L

C

A

A

L

L

L

E

E

G

G

A

G

E

E

D

A

A

A

R

V

A

V

T

A

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

A

A

A

S

I

L

I

W

C

S

Q

A

V

L

K

K

S

S

G

G

D

D

H

H

V

V

L

V

A

A

A

S

R

D

A

T

M

L

F
x
Y

G

G

S

C

C

T

R

F

Y

A

I

L

E

N

T

H

L

G

L

L

P

T

R

R

Y

Y

G

G

V

V

E

E

F

V

T

T

L

F

I

V

D

D

G

V

S

S

K

N

I

L

E

D

N

E

W

V

Q

K

K

N

A

A

V

L

R

K

P

P

N

N

T

T

K

K

V

V

M

V

F

Y

L

L

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

K

V

V

I

T

D

D

I

I

A

R

A

K

V

I

A

S

N

N

I

M

A

V

N

H

S

E

V

S

G

N

A

K

K

E

-

C

-

F

L

V

I

F

V

V

D
|
D

N

N

V

T

F
|
F

A

C

T

T

P

P

L

Y

F

I

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

V

V

V

Y

H

S
|
S

T

A

T
|
T

K
|
K

H

Y

I

L

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

A

G

G

V

F

I

A

L

V

S

G

D

K

K

E

Q

E

W

F

V

I

D

N

E

Q

-

V

N

K

L

L

H

F

E

G

Y

I

F

K

R

D

H

M

T

T

G

G

P

S

G

V

L

T

S

G

P

P

F

F

N

E

S

S

W

F

V

L

M

I

L

I

K

R

G

G

M

M

E

K

T

T

L

L

V

Q

V

L

R

R

V

M

R

E

Q

K

Q

H

T

C

E

K

S

N

A

A

G

M

K

E

I

V

A

A

D

K

F

F

L

L

A

E

D

S

H

H

P

P

A

A

V

V

S

E

K

K

V

V

I

Y

Y

Y

P

P

G

G

R

L

P

E

D

S

H

F

P

K

Q

Y

A

Y

D

Q

I

L

I

A

A

R

R

E

Q

Q

M

M

S

K

G

L

G

P

S

G

T

A

L

M

I

I

A

S

F

F

E

E

V

L

K

K

G

G

K

V

K

E

A

E

T

G

F

K

N

I

A

V

E

M

N

N

K

N

L

V

E

K

I

L

I

A

Q

T

I

L

S

A

N

V

N

S

L

L

G

G

D

D

S

S

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

P

L

Y

T

T

D

A

D

E

A

E

R

R

A

K

E

A

L

A

G

G

I

I

S

S

D

D

G

G

L

L

L

V

R

R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

A

D

E

D

D

L

I

I

I

E

D

D

D

L

L

D

K

V

Q

A

A

L

L

active site: R59 (= R61), Y112 (≠ F114), D186 (= D186), K211 (= K211)
binding pyridoxal-5'-phosphate: Y57 (= Y59), R59 (= R61), S86 (= S88), G87 (= G89), M88 (= M90), Y112 (≠ F114), D186 (= D186), F189 (= F189), S208 (= S208), T210 (= T210), K211 (= K211)

3vk3A Crystal structure of l-methionine gamma-lyase from pseudomonas putida c116h mutant complexed with l-methionine (see paper)
39% identity, 92% coverage: 32:396/397 of query aligns to 31:396/397 of 3vk3A

query
sites
3vk3A

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

P

P

S

T

A

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

E

Q

P

A

G

G

F

H

I

F

Y

Y

S

S

R
|
R

Y

I

A

S

N

N

P

P

T

T

T

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

A

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G

G

M

M

A

G

A

A

V

I

A

T

A

S

A

T

I

L

I

W

C

T

Q

L

V

L

K

R

S

P

G

G

D

D

H

E

V

V

L

L

A

L

A

G

R

N

A

T

M

L

F
x
Y

G

G

S

H

C

T

R

F

Y

A

I

F

I

L

E

H

T

H

L

G

L

I

P

G

R

E

Y

F

G

G

V

V

E

K

F

L

T

R

L

H

I

V

D

D

G

M

S

A

K

D

I

L

E

Q

N

A

W

L

Q

E

K

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

M

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

N

K

I

I

A

A

N

R

S

K

V

H

G

G

A

A

K

T

L

V

I

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S

S

T

A

T

T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

D

S

K

Q

Q

A

W

L

V

V

D

D

E

R

-

I

N

R

L

L

H

Q

E

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

G

V

L

L

S

S

P

P

F

H

N

D

S

A

W

A

V

L

M

L

L

M

K

R

G

G

M

I

E

K

T

T

L

L

V

N

V

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

G

Q

K

V

I

L

A

A

D

E

F

F

L

L

A

A

D

R

H

Q

P

P

A

Q

V

V

S

E

K

L

V

I

I

H

Y

Y

P

P

G

G

R

L

P

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

R

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

I

I

A

A

F

F

E

E

V

L

K

K

G

G

K

I

K

G

A

A

T

G

F

R

N

R

A

F

E

M

N

N

K

A

L

L

E

Q

I

L

I

F

Q

S

I

R

S

A

N
x
V

N
x
S

L
|
L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

D

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

I

D

D

L

L

I

L

E

A

D

D

L

V

D

Q

V

Q

A

A

L

L

K

K

G

A

A

S

active site: R60 (= R61), Y113 (≠ F114), D185 (= D186), K210 (= K211)
binding methionine: Y113 (≠ F114), K210 (= K211), V338 (≠ N338), S339 (≠ N339), L340 (= L340), R374 (= R374)

5x2xA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-homocysteine intermediates (see paper)
39% identity, 92% coverage: 32:396/397 of query aligns to 26:391/392 of 5x2xA

query
sites
5x2xA

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

P

P

S

T

A

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

E

Q

P

A

G

G

F

H

I

F

Y
|
Y

S

S

R
|
R

Y

I

A

S

N

N

P

P

T

T

T

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

A

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G
|
G

M
|
M

A

G

A

A

V

I

A

T

A

S

A

T

I

L

I

W

C

T

Q

L

V

L

K

R

S

P

G

G

D

D

H

E

V

V

L

L

A

L

A

G

R

N

A

T

M

L

F
x
Y

G

G

S

C

C

T

R

F

Y

A

I

F

I

L

E

H

T

H

L

G

L

I

P

G

R

E

Y

F

G

G

V

V

E

K

F

L

T

R

L

H

I

V

D

D

G

M

S

A

K

D

I

L

E

Q

N

A

W

L

Q

E

K

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

M

I

F

Y

L

F

E

E

S

S

P

P

T

A

N
|
N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

N

K

I

I

A

A

N

R

S

K

V

H

G

G

A

A

K

T

L

V

I

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

T

A

T
|
T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

D

S

K

Q

Q

A

W

L

V

V

D

D

E

R

-

I

N

R

L

L

H

Q

E

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

G

V

L

L

S

S

P

P

F

H

N

D

S

A

W

A

V

L

M

L

L

M

K

R

G

G

M

I

E

K

T

T

L

L

V

N

V

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

G

Q

K

V

I

L

A

A

D

E

F

F

L

L

A

A

D

R

H

Q

P

P

A

Q

V

V

S

E

K

L

V

I

I

H

Y

Y

P

P

G

G

R

L

P

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

R

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

I

I

A

A

F

F

E

E

V

L

K

K

G

G

K

I

K

G

A

A

T

G

F

R

N

R

A

F

E

M

N

N

K

A

L

L

E

Q

I

L

I

F

Q

S

I

R

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

D

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

I

D

D

L

L

I

L

E

A

D

D

L

V

D

Q

V

Q

A

A

L

L

K

K

G

A

A

S

active site: R55 (= R61), Y108 (≠ F114), D180 (= D186), K205 (= K211)
binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: Y53 (= Y59), R55 (= R61), G83 (= G89), M84 (= M90), Y108 (≠ F114), N155 (= N161), D180 (= D186), S202 (= S208), T204 (= T210), K205 (= K211), V333 (≠ N338), S334 (≠ N339), R369 (= R374)

5x2wA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-methionine intermediates (see paper)
39% identity, 92% coverage: 32:396/397 of query aligns to 26:391/392 of 5x2wA

query
sites
5x2wA

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

P

P

S

T

A

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

E

Q

P

A

G

G

F

H

I

F

Y
|
Y

S

S

R
|
R

Y

I

A

S

N

N

P

P

T

T

T

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

A

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

A

T

A

S

A

T

I

L

I

W

C

T

Q

L

V

L

K

R

S

P

G

G

D

D

H

E

V

V

L

L

A

L

A

G

R

N

A

T

M

L

F
x
Y

G

G

S

C

C

T

R

F

Y

A

I

F

I

L

E

H

T

H

L

G

L

I

P

G

R

E

Y

F

G

G

V

V

E

K

F

L

T

R

L

H

I

V

D

D

G

M

S

A

K

D

I

L

E

Q

N

A

W

L

Q

E

K

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

M

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

N

K

I

I

A

A

N

R

S

K

V

H

G

G

A

A

K

T

L

V

I

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

T

A

T

T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

D

S

K

Q

Q

A

W

L

V

V

D

D

E

R

-

I

N

R

L

L

H

Q

E

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

G

V

L

L

S

S

P

P

F

H

N

D

S

A

W

A

V

L

M

L

L

M

K

R

G

G

M

I

E

K

T

T

L

L

V

N

V

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

G

Q

K

V

I

L

A

A

D

E

F

F

L

L

A

A

D

R

H

Q

P

P

A

Q

V

V

S

E

K

L

V

I

I

H

Y

Y

P

P

G

G

R

L

P

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

R

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

I

I

A

A

F

F

E

E

V

L

K

K

G

G

K

I

K

G

A

A

T

G

F

R

N

R

A

F

E

M

N

N

K

A

L

L

E

Q

I

L

I

F

Q

S

I

R

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

D

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

I

D

D

L

L

I

L

E

A

D

D

L

V

D

Q

V

Q

A

A

L

L

K

K

G

A

A

S

active site: R55 (= R61), Y108 (≠ F114), D180 (= D186), K205 (= K211)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid: Y53 (= Y59), R55 (= R61), S82 (= S88), G83 (= G89), M84 (= M90), Y108 (≠ F114), D180 (= D186), S202 (= S208), K205 (= K211), V333 (≠ N338), S334 (≠ N339), R369 (= R374)

1pg8A Crystal structure of l-methionine alpha-, gamma-lyase
39% identity, 92% coverage: 32:396/397 of query aligns to 32:397/398 of 1pg8A

query
sites
1pg8A

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

P

P

S

T

A

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

E

Q

P

A

G

G

F

H

I

F

Y
|
Y

S

S

R
|
R

Y

I

A

S

N

N

P

P

T

T

T

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

A

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

A

T

A

S

A

T

I

L

I

W

C

T

Q

L

V

L

K

R

S

P

G

G

D

D

H

E

V

V

L

L

A

L

A

G

R

N

A

T

M

L

F
x
Y

G

G

S

C

C

T

R

F

Y

A

I

F

I

L

E

H

T

H

L

G

L

I

P

G

R

E

Y

F

G

G

V

V

E

K

F

L

T

R

L

H

I

V

D

D

G

M

S

A

K

D

I

L

E

Q

N

A

W

L

Q

E

K

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

M

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

N

K

I

I

A

A

N

R

S

K

V

H

G

G

A

A

K

T

L

V

I

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

T

A

T
|
T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

D

S

K

Q

Q

A

W

L

V

V

D

D

E

R

-

I

N

R

L

L

H

Q

E

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

G

V

L

L

S

S

P

P

F

H

N

D

S

A

W

A

V

L

M

L

L

M

K

R

G

G

M

I

E

K

T

T

L

L

V

N

V

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

G

Q

K

V

I

L

A

A

D

E

F

F

L

L

A

A

D

R

H

Q

P

P

A

Q

V

V

S

E

K

L

V

I

I

H

Y

Y

P

P

G

G

R

L

P

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

R

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

I

I

A

A

F

F

E

E

V

L

K

K

G

G

K

I

K

G

A

A

T

G

F

R

N

R

A

F

E

M

N

N

K

A

L

L

E

Q

I

L

I

F

Q

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

D

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R

R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

I

D

D

L

L

I

L

E

A

D

D

L

V

D

Q

V

Q

A

A

L

L

K

K

G

A

A

S

active site: R61 (= R61), Y114 (≠ F114), D186 (= D186), K211 (= K211)
binding pyridoxal-5'-phosphate: Y59 (= Y59), R61 (= R61), S88 (= S88), G89 (= G89), M90 (= M90), Y114 (≠ F114), D186 (= D186), S208 (= S208), T210 (= T210), K211 (= K211)

P13254 L-methionine gamma-lyase; MGL; Homocysteine desulfhydrase; L-methioninase; EC 4.4.1.11; EC 4.4.1.2 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
39% identity, 92% coverage: 32:396/397 of query aligns to 32:397/398 of P13254

query
sites
P13254

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

P

P

S

T

A

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

E

Q

P

A

G

G

F

H

I

F

Y
|
Y

S
|
S

R
|
R

Y

I

A

S

N

N

P

P

T

T

T

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

A

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G
|
G

M
|
M

A

G

A

A

V

I

A

T

A

S

A

T

I

L

I

W

C

T

Q

L

V

L

K

R

S

P

G

G

D

D

H

E

V

V

L

L

A

L

A

G

R

N

A

T

M

L

F
x
Y

G

G

S
x
C

C

T

R

F

Y

A

I

F

I

L

E

H

T

H

L

G

L

I

P

G

R

E

Y

F

G

G

V

V

E

K

F

L

T

R

L

H

I

V

D

D

G

M

S

A

K

D

I

L

E

Q

N

A

W

L

Q

E

K

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

M

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

N

K

I

I

A

A

N

R

S

K

V

H

G

G

A

A

K

T

L

V

I

V

V

V

D

D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

T
x
A

T
|
T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

D

S

K

Q

Q

A

W

L

V

V

D

D

E

R

-

I

N

R

L

L

H

Q

E

G

Y

L

F
x
K

R
x
D

H

M

T

T

G

G

P

A

G

V

L

L

S

S

P

P

F

H

N

D

S

A

W

A

V

L

M

L

L

M

K

R

G

G

M

I

E

K

T

T

L

L

V

N

V

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

G

Q

K

V

I

L

A

A

D

E

F

F

L

L

A

A

D

R

H

Q

P

P

A

Q

V

V

S

E

K

L

V

I

I

H

Y

Y

P

P

G

G

R

L

P

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

R

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

I

I

A

A

F

F

E

E

V

L

K

K

G

G

K

I

K

G

A

A

T

G

F

R

N

R

A

F

E

M

N

N

K

A

L

L

E

Q

I

L

I

F

Q

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

D

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

I

D

D

L

L

I

L

E

A

D

D

L

V

D

Q

V

Q

A

A

L

L

K

K

G

A

A

S

YSR 59:61 (= YSR 59:61) binding pyridoxal 5'-phosphate
R61 (= R61) mutation R->A,E,F: Loss of elimination activity against L-methionine.
GM 89:90 (= GM 89:90) binding in other chain
Y114 (≠ F114) binding substrate
C116 (≠ S116) mutation to H: Drastic decrease of the catalytic efficiency of the elimination reaction with L-methionine, by 6700-fold, and increases that with L-cysteine by 7-fold, mainly due to changes in kcat. Loss of ability to catalyze replacement reaction between L-methionine and 2-mercaptoethanol.; mutation to S: 9% of wild-type elimination activity against L-methionine.; mutation to T: 40% of wild-type elimination activity against L-methionine.
SAT 208:210 (≠ STT 208:210) binding in other chain
K211 (= K211) modified: N6-(pyridoxal phosphate)lysine
K240 (≠ F239) mutation K->D,E: Marked decrease in elimination activity against both L-methionine and DL-homocysteine.; mutation to M: 50% reduction in alpha,gamma-elimination activity against DL-homocysteine, while retaining elimination activity against L-methionine and L-cysteine.
D241 (≠ R240) mutation D->H,R: 5 to 14-fold reduction in alpha,gamma-elimination activity against L-methionine, while no change in affinity for L-methionine.
R375 (= R374) binding substrate

5x30C Crystal structure of pseudomonas putida methionine gamma-lyase c116h mutant with l-homocysteine intermediates. (see paper)
39% identity, 92% coverage: 32:396/397 of query aligns to 27:392/393 of 5x30C

query
sites
5x30C

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

P

P

S

T

A

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

E

Q

P

A

G

G

F

H

I

F

Y
|
Y

S

S

R
|
R

Y

I

A

S

N

N

P

P

T

T

T

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

A

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G

G

M

M

A

G

A

A

V

I

A

T

A

S

A

T

I

L

I

W

C

T

Q

L

V

L

K

R

S

P

G

G

D

D

H

E

V

V

L

L

A

L

A

G

R

N

A

T

M

L

F
x
Y

G

G

S

H

C

T

R

F

Y

A

I

F

I

L

E

H

T

H

L

G

L

I

P

G

R

E

Y

F

G

G

V

V

E

K

F

L

T

R

L

H

I

V

D

D

G

M

S

A

K

D

I

L

E

Q

N

A

W

L

Q

E

K

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

M

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

N

K

I

I

A

A

N

R

S

K

V

H

G

G

A

A

K

T

L

V

I

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S

S

T

A

T

T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

D

S

K

Q

Q

A

W

L

V

V

D

D

E

R

-

I

N

R

L

L

H

Q

E

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

G

V

L

L

S

S

P

P

F

H

N

D

S

A

W

A

V

L

M

L

L

M

K

R

G

G

M

I

E

K

T

T

L

L

V

N

V

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

G

Q

K

V

I

L

A

A

D

E

F

F

L

L

A

A

D

R

H

Q

P

P

A

Q

V

V

S

E

K

L

V

I

I

H

Y

Y

P

P

G

G

R

L

P

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

R

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

I

I

A

A

F

F

E

E

V

L

K

K

G

G

K

I

K

G

A

A

T

G

F

R

N

R

A

F

E

M

N

N

K

A

L

L

E

Q

I

L

I

F

Q

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

D

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R

R

L

L

S

S

V

V

G

G

L

L

E

E

D

D

T

I

D

D

L

L

I

L

E

A

D

D

L

V

D

Q

V

Q

A

A

L

L

K

K

G

A

A

S

active site: R56 (= R61), Y109 (≠ F114), D181 (= D186), K206 (= K211)
binding (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid: Y54 (= Y59), R56 (= R61)

1e5fA Methionine gamma-lyase (mgl) from trichomonas vaginalis
35% identity, 98% coverage: 7:397/397 of query aligns to 1:393/393 of 1e5fA

query
sites
1e5fA

R

E

K

R

L

M

R

T

P

P

A

A

T

T

Q

A

L

C

V

I

H

H

A

A

G

N

T

P

L

Q

R

K

S

D

G

Q

F

F

G

G

E

A

T

A

S

I

E

P

A

P

L

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

N

A

C

E

Q

A

Q

A

G

E

G

A

N

R

R

F

F

K

A

G

G

E

Q

E

E

P

S

G

G

F

Y

I

I

Y
|
Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

T

V

D

S

M

N

F

L

E

E

K

G

R

K

M

I

C

A

A

F

L

L

E

E

G

K

A

T

E

E

D

A

A

C

R

V

A

A

T

T

A

S

S

S

G
|
G

M
|
M

A

G

A

A

V

I

A

A

A

T

I

V

I

L

C

T

Q

I

V

L

K

K

S

A

G

G

D

D

H

H

V

L

L

I

A

S

A

D

R

E

A

C

M

L

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

L

I

F

E

E

T

H

L

A

L

L

P

T

R

K

Y

F

G

G

V

I

E

Q

F

V

T

D

L

F

I

I

D

N

G

T

S

A

K

I

I

P

E

G

N

E

W

V

Q

K

K

K

A

H

V

M

R

K

P

P

N

N

T

T

K

K

V

I

M

V

F

Y

L

F

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

K

V

I

I

I

D

D

I

M

A

E

A

R

V

V

A

C

N

K

I

D

A

A

N

H

S

S

V

Q

-

E

G

G

A

V

K

L

L

V

I

I

V

A

D
|
D

N

N

V

T

F

F

A

C

T

S

P

P

L

M

F

I

Q

T

K

N

P

P

L

V

E

D

L

F

G

G

A

V

H

D

I

V

V

V

Y

H

S
|
S

T

A

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

T

R

D

C

V

L

V

G

A

G

G

V

L

I

I

L

C

S

G

D

K

K

A

Q

D

W

L

V

L

D

Q

E

Q

N

I

L

R

H

M

E

V

Y

G

F

I

R

K

H

D

-

I

T

T

G

G

P

S

G

V

L

I

S

S

P

P

F

H

N

D

S

A

W

W

V

L

M

I

L

T

K

R

G

G

M

L

E

S

T

T

L

L

V

N

V

I

R

R

V

M

R

K

Q

A

Q

E

T

S

E

E

S

N

A

A

G

M

K

K

I

V

A

A

D

E

F

Y

L

L

A

K

D

S

H

H

P

P

A

A

V

V

S

E

K

K

V

V

I

Y

Y

Y

P

P

G

G

R

F

P

E

D

D

H

H

P

E

Q

G

A

H

D

D

I

I

I

A

A

K

R

K

Q

Q

M

M

S

R

G

M

G

Y

S

G

T

S

L

M

I

I

A

T

F

F

E

I

V

L

K

K

G

S

G

G

K

F

K

E

A

G

T

A

F

K

N

K

A

L

E

L

N

D

K

N

L

L

E

K

I

L

I

I

Q

T

I

L

S

A

N

V

N

S

L

L

G

G

D

G

S

C

K

E

S

S

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T

T

H

H

K

A

N

V

L

V

T

P

D

K

D

E

A

E

R

R

A

E

E

A

L

A

G

G

I

I

S

T

D

D

G

G

L

M

L

I

R

R

L

L

S

S

V

V

G

G

L

I

E

E

D

D

T

A

D

D

D

E

L

L

I

I

E

A

D

D

L

F

D

K

V

Q

A

G

L

L

K

D

G

A

A

L

L

L

active site: R55 (= R61), Y108 (≠ F114), D181 (= D186), K206 (= K211)
binding pyridoxal-5'-phosphate: Y53 (= Y59), R55 (= R61), G83 (= G89), M84 (= M90), Y108 (≠ F114), D181 (= D186), S203 (= S208), K206 (= K211)

1e5eA Methionine gamma-lyase (mgl) from trichomonas vaginalis in complex with propargylglycine
35% identity, 98% coverage: 7:397/397 of query aligns to 1:393/394 of 1e5eA

query
sites
1e5eA

R

E

K

R

L

M

R

T

P

P

A

A

T

T

Q

A

L

C

V

I

H

H

A

A

G

N

T

P

L

Q

R

K

S

D

G

Q

F

F

G

G

E

A

T

A

S

I

E

P

A

P

L

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

P

D

S

N

A

C

E

Q

A

Q

A

G

E

G

A

N

R

R

F

F

K

A

G

G

E

Q

E

E

P

S

G

G

F

Y

I

I

Y
|
Y

S

T

R
|
R

Y

L

A

G

N

N

P

P

T

T

T

V

D

S

M

N

F

L

E

E

K

G

R

K

M

I

C

A

A

F

L

L

E

E

G

K

A

T

E

E

D

A

A

C

R

V

A

A

T

T

A

S

S

S

G
|
G

M
|
M

A

G

A

A

V

I

A

A

A

T

I

V

I

L

C

T

Q

I

V

L

K

K

S

A

G

G

D

D

H

H

V

L

L

I

A

S

A

D

R

E

A

C

M

L

F
x
Y

G

G

S

C

C

T

R

H

Y

A

I

L

I

F

E

E

T

H

L

A

L

L

P

T

R

K

Y

F

G

G

V

I

E

Q

F

V

T

D

L

F

I

I

D

N

G

T

S

A

K

I

I

P

E

G

N

E

W

V

Q

K

K

K

A

H

V

M

R

K

P

P

N

N

T

T

K

K

V

I

M

V

F

Y

L

F

E

E

S

T

P

P

T

A

N
|
N

P

P

T

T

L

L

E

K

V

I

I

I

D

D

I

M

A

E

A

R

V

V

A

C

N

K

I

D

A

A

N

H

S

S

V

Q

-

E

G

G

A

V

K

L

L

V

I

I

V

A

D
|
D

N

N

V

T

F

F

A

C

T

S

P

P

L

M

F

I

Q

T

K

N

P

P

L

V

E

D

L

F

G

G

A

V

H

D

I

V

V

V

Y

H

S
|
S

T

A

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

T

R

D

C

V

L

V

G

A

G

G

V

L

I

I

L

C

S

G

D

K

K

A

Q

D

W

L

V

L

D

Q

E

Q

N

I

L

R

H

M

E

V

Y

G

F

I

R

K

H

D

-

I

T

T

G

G

P

S

G

V

L

I

S

S

P

P

F

H

N

D

S

A

W

W

V

L

M

I

L

T

K

R

G

G

M

L

E

S

T

T

L

L

V

N

V

I

R

R

V

M

R

K

Q

A

Q

E

T

S

E

E

S

N

A

A

G

M

K

K

I

V

A

A

D

E

F

Y

L

L

A

K

D

S

H

H

P

P

A

A

V

V

S

E

K

K

V

V

I

Y

Y

Y

P

P

G

G

R

F

P

E

D

D

H

H

P

E

Q

G

A

H

D

D

I

I

I

A

A

K

R

K

Q

Q

M

M

S

R

G

M

G

Y

S

G

T

S

L

M

I

I

A

T

F

F

E

I

V

L

K

K

G

S

G

G

K

F

K

E

A

G

T

A

F

K

N

K

A

L

E

L

N

D

K

N

L

L

E

K

I

L

I

I

Q

T

I

L

S

A

N

V

N
x
S

L

L

G

G

D

G

S

C

K

E

S

S

L

L

I

I

T

Q

H

H

P

P

T

A

T

S

T

M

T
|
T

H

H

K

A

N

V

L

V

T

P

D

K

D

E

A

E

R

R

A

E

E

A

L

A

G

G

I

I

S

T

D

D

G

G

L

M

L

I

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

D

D

T

A

D

D

D

E

L

L

I

I

E

A

D

D

L

F

D

K

V

Q

A

G

L

L

K

D

G

A

A

L

L

L

active site: R55 (= R61), Y108 (≠ F114), D181 (= D186), K206 (= K211)
binding n-(hydroxy{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)norvaline: Y53 (= Y59), R55 (= R61), G83 (= G89), M84 (= M90), Y108 (≠ F114), N155 (= N161), D181 (= D186), S203 (= S208), T205 (= T210), K206 (= K211), S335 (≠ N339), T350 (= T354), R370 (= R374)

3aeoA Reaction intermediate structure of entamoeba histolytica methionine gamma-lyase 1 containing methionine alpha, beta-enamine-pyridoxamine- 5'-phosphate
37% identity, 91% coverage: 32:394/397 of query aligns to 24:385/387 of 3aeoA

query
sites
3aeoA

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

C

Y

F

P

D

S

S

A

T

E

Q

A

Q

A

G

E

A

A

D

R

L

F

F

K

M

G

G

E

K

E

G

P

E

G

G

F

H

I

I

Y
|
Y

S

S

R
|
R

Y

L

A

G

N

N

P

P

T

T

T

V

D

E

M

Q

F

F

E

E

K

E

R

M

M

V

C

C

A

S

L

I

E

E

G

G

A

A

E

A

D

G

A

S

R

A

A

A

T

F

A

G

S

S

G
|
G

M
|
M

A

G

A

A

V

I

A

S

I

T

I

L

C

A

Q

F

V

L

K

Q

S

K

G

G

D

D

H

H

V

L

L

I

A

A

A

G

R

D

A

T

M

L

F
x
Y

G

G

S

C

C

T

R

V

Y

S

I

L

I

F

E

T

T

H

L

W

L

L

P

P

R

R

Y

F

G

G

V

I

E

E

F

V

T

D

L

L

I

I

D

D

G

T

S

S

K

D

I

V

E

E

N

K

W

V

Q

K

K

A

A

A

V

W

R

K

P

P

N

N

T

T

K

K

V

M

M

V

F

Y

L

L

E
|
E

S

S

P

P

T

A

N
|
N

P

P

T

T

L

C

E

K

V

V

I

S

D

D

I

I

A

K

A

G

V

I

A

A

N

V

I

V

A

C

N

H

S

E

V

R

G

G

A

A

K

R

L

L

I

V

V

V

D
|
D

N

A

V

T

F

F

A

T

T

S

P

P

L

C

F

F

Q

L

K

K

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

I

V

A

V

L

Y

H

S
|
S

T

V

T
x
S

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

V

I

S

L

S

S

A

D

K

K

T

Q

A

W

E

V

D

D

I

E

A

N

T

L

I

H

K

E

F

Y

Y

F

R

R

K

H

D

T

A

G

G

P

S

G

L

L

M

S

A

P

P

F

M

N

D

S

A

W

F

V

L

M

C

L

A

K

R

G

G

M

M

E

K

T

T

L

L

V

P

V

I

R

R

V

M

R

Q

Q

I

Q

H

T

M

E

E

S

N

A

G

G

L

K

K

I

V

A

A

D

K

F

F

L

L

A

E

D

Q

H

H

P

E

A

K

V

I

S

V

K

K

V

V

I

N

Y

H

P

P

G

G

R

L

P

E

D

S

H

F

P

P

Q

G

A

H

D

D

I

I

I

A

A

K

R

K

Q

Q

M

M

S

T

G

G

G

Y

S

G

T

S

L

T

I

F

A

L

F

F

E

E

V

M

K

K

G

S

G

F

K

E

K

A

A

A

T

K

F

K

N

L

A

M

E

E

N

H

K

-

L

L

E

K

I

V

I

C

Q

T

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

C

S

V

K

D

S

T

L

L

I

I

T

E

H

H

P

P

T

A

T

S

T

M

T
|
T

H

H

K

A

N

A

L

V

T

P

D

E

D

N

A

I

R

M

A

R

E

K

L

Q

G

G

I

I

S

T

D

P

G

E

L

L

L

V

R
|
R

L

I

S

S

V

V

G

G

L

I

E

E

D

N

T

V

D

D

L

I

I

I

E

A

D

D

L

L

D

K

V

Q

A

A

L

L

K

E

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid: Y51 (= Y59), R53 (= R61), G81 (= G89), M82 (= M90), Y106 (≠ F114), E149 (= E157), N153 (= N161), D178 (= D186), S200 (= S208), S202 (≠ T210), K203 (= K211), V329 (≠ N338), S330 (≠ N339), T345 (= T354), R365 (= R374)

3aelA Reaction intermediate structure of entamoeba histolytica methionine gamma-lyase 1 containing methionine imine-pyridoxamine-5'-phosphate and alpha-amino-alpha, beta-butenoic acid-pyridoxal-5'-phosphate
37% identity, 91% coverage: 32:394/397 of query aligns to 24:385/387 of 3aelA

query
sites
3aelA

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

C

Y

F

P

D

S

S

A

T

E

Q

A

Q

A

G

E

A

A

D

R

L

F

F

K

M

G

G

E

K

E

G

P

E

G

G

F

H

I

I

Y
|
Y

S

S

R
|
R

Y

L

A

G

N

N

P

P

T

T

T

V

D

E

M

Q

F

F

E

E

K

E

R

M

M

V

C

C

A

S

L

I

E

E

G

G

A

A

E

A

D

G

A

S

R

A

A

A

T

F

A

G

S

S

G
|
G

M
|
M

A

G

A

A

V

I

A

S

I

T

I

L

C

A

Q

F

V

L

K

Q

S

K

G

G

D

D

H

H

V

L

L

I

A

A

A

G

R

D

A

T

M

L

F
x
Y

G

G

S

C

C

T

R

V

Y

S

I

L

I

F

E

T

T

H

L

W

L

L

P

P

R

R

Y

F

G

G

V

I

E

E

F

V

T

D

L

L

I

I

D

D

G

T

S

S

K

D

I

V

E

E

N

K

W

V

Q

K

K

A

A

A

V

W

R

K

P

P

N

N

T

T

K

K

V

M

M

V

F

Y

L

L

E
|
E

S

S

P

P

T

A

N
|
N

P

P

T

T

L

C

E

K

V

V

I

S

D

D

I

I

A

K

A

G

V

I

A

A

N

V

I

V

A

C

N

H

S

E

V

R

G

G

A

A

K

R

L

L

I

V

V

V

D
|
D

N

A

V
x
T

F

F

A

T

T

S

P

P

L

C

F

F

Q

L

K

K

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

I

V

A

V

L

Y

H

S
|
S

T

V

T
x
S

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

V

I

S

L

S

S

A

D

K

K

T

Q

A

W

E

V

D

D

I

E

A

N

T

L

I

H

K

E

F

Y

Y

F

R

R

K

H

D

T

A

G

G

P

S

G

L

L

M

S

A

P

P

F

M

N

D

S

A

W

F

V

L

M

C

L

A

K

R

G

G

M

M

E

K

T

T

L

L

V

P

V

I

R

R

V

M

R

Q

Q

I

Q

H

T

M

E

E

S

N

A

G

G

L

K

K

I

V

A

A

D

K

F

F

L

L

A

E

D

Q

H

H

P

E

A

K

V

I

S

V

K

K

V

V

I

N

Y

H

P

P

G

G

R

L

P

E

D

S

H

F

P

P

Q

G

A

H

D

D

I

I

I

A

A

K

R

K

Q

Q

M

M

S

T

G

G

G

Y

S

G

T

S

L

T

I

F

A

L

F

F

E

E

V

M

K

K

G

S

G

F

K

E

K

A

A

A

T

K

F

K

N

L

A

M

E

E

N

H

K

-

L

L

E

K

I

V

I

C

Q

T

I

L

S

A

N

V

N
x
S

L

L

G

G

D

C

S

V

K

D

S

T

L

L

I

I

T

E

H

H

P

P

T

A

T

S

T

M

T
|
T

H

H

K

A

N

A

L

V

T

P

D

E

D

N

A

I

R

M

A

R

E

K

L

Q

G

G

I

I

S

T

D

P

G

E

L

L

L

V

R
|
R

L

I

S

S

V

V

G

G

L

I

E

E

D

N

T

V

D

D

L

I

I

I

E

A

D

D

L

L

D

K

V

Q

A

A

L

L

K

E

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]-4-(methylsulfanyl)butanoic acid: Y51 (= Y59), R53 (= R61)
binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: G81 (= G89), M82 (= M90), Y106 (≠ F114), E149 (= E157), N153 (= N161), D178 (= D186), T180 (≠ V188), S200 (= S208), S202 (≠ T210), K203 (= K211), S330 (≠ N339), T345 (= T354), R365 (= R374)

Query Sequence

>WP_106716016.1 NCBI__GCF_003010935.1:WP_106716016.1
MTKNTTRKLRPATQLVHAGTLRSGFGETSEALFLTQGFVYPSAEAAEARFKGEEPGFIYS
RYANPTTDMFEKRMCALEGAEDARATASGMAAVAAAIICQVKSGDHVLAARAMFGSCRYI
IETLLPRYGVEFTLIDGSKIENWQKAVRPNTKVMFLESPTNPTLEVIDIAAVANIANSVG
AKLIVDNVFATPLFQKPLELGAHIVVYSTTKHIDGQGRCLGGVILSDKQWVDENLHEYFR
HTGPGLSPFNSWVMLKGMETLVVRVRQQTESAGKIADFLADHPAVSKVIYPGRPDHPQAD
IIARQMSGGSTLIAFEVKGGKKATFNAENKLEIIQISNNLGDSKSLITHPTTTTHKNLTD
DARAELGISDGLLRLSVGLEDTDDLIEDLDVALKGAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory