SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
26% identity, 88% coverage: 14:239/257 of query aligns to 4:231/254 of 1g6hA

query
sites
1g6hA

V

I

L

L

S

R

V

T

S

E

G

N

A

I

C

V

K

K

R

Y

F
|
F

G

G

S

E

V
x
F

V

K

A

A

L

L

D

D

N

G

V

V

S

S

I

I

D

S

V

V

H

N

R

K

G

G

E

D

I

V

L

T

A

L

L

I

G

G

D

P

N
|
N

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

N

C

V

I

I

S

T

G

G

V

F

H

L

R

K

L

A

D

D

A

E

G

G

D

R

V

V

V

Y

I

F

D

E

G

N

A

K

A

D

N

I

V

T

L

N

R

K

S

E

P

P

A

A

D

E

A

L

R

Y

L

H

A

Y

G

G

I

I

E

V

T

R

V

T

Y

F

Q

Q

D

T

L

P

A

Q

L

P

F

L

D

K

N

E

L

M

T

T

P

V

A

L

Q

E

N

N

F

L

A

I

G

G

R

E

E

I

I

C

A

P

G

G

-

E

-

S

-

P

-

L

-

N

-

S

-

L

-

F

-

Y

P

K

R

K

W

W

F

I

P

P

R

K

A

-

M

-

R

-

F

-

L

-

N

-

K

E

R

E

K

E

M

M

D

V

E

E

Q

K

T

A

R

F

S

K

L

I

I

L

Q

E

S

F

L

L

N

K

V

L

S

S

L

-

P

-

H

H

L

L

-

Y

D

D

A

R

V

K

V

A

A

G

L

E

M

L

S

S

G

G

Q

Q

R

M

Q

K

A

L

I

V

A

E

V

I

A

G

R

R

S

A

A

L

V

M

F

T

A

N

R

P

K

K

V

M

V

I

I

V

L

M

D

D

E

E

P

P

T

I

A

A

A

G

L

V

G

A

Q

P

R

G

E

L

S

A

R

H

K

D

V

I

L

F

D

N

L

H

I

V

G

L

A

E

L

L

R

K

D

A

R

K

G

G

N

I

A

T

I

F

I

L

L

I

I

I

T

E

H

H

N

R

M

L

E

D

H

I

V

V

T

L

E

N

L

Y

A

I

C

D

R

H

A

L

V

Y

V

V

L

M

R

F

Q

N

G

G

R

Q

R

I

V

I

G

A

E

E

binding adenosine-5'-diphosphate: F14 (= F24), F17 (≠ V27), N39 (= N49), G40 (= G50), G42 (= G52), K43 (= K53), S44 (= S54), T45 (= T55)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 86% coverage: 15:235/257 of query aligns to 3:210/371 of 3puyA

query
sites
3puyA

L

V

S

Q

V

L

S

Q

G

N

A

V

C

T

K

K

R

A

F
x
W

G

G

S

E

V

V

A

V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

E

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

K

N

R

V

M

L

N

R

D

S

T

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R

K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

I

D

N

E

Q

Q

R

T

V

R

N

S

Q

L

V

I

A

Q

E

S

V

L

L

N

Q

V

-

S

-

L

L

P

A

H

H

-

L

L

L

D

D

A
x
R

V

K

V

P

A

K

L
x
A

M

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

S

V

V

V

F

I

L

L

L

D

D

E
|
E

P

P

T

L

A

S

A

N

L

L

G

D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H
|
H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F24), S37 (≠ N49), G38 (= G50), C39 (≠ A51), G40 (= G52), K41 (= K53), S42 (= S54), T43 (= T55), Q81 (= Q96), R128 (≠ A154), A132 (≠ L158), S134 (= S160), G136 (= G162), Q137 (= Q163), E158 (= E184), H191 (= H216)
binding magnesium ion: S42 (= S54), Q81 (= Q96)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 86% coverage: 15:235/257 of query aligns to 3:210/371 of 3puxA

query
sites
3puxA

L

V

S

Q

V

L

S

Q

G

N

A

V

C

T

K

K

R

A

F
x
W

G

G

S

E

V

V

A

V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

E

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

K

N

R

V

M

L

N

R

D

S

T

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R

K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

I

D

N

E

Q

Q

R

T

V

R

N

S

Q

L

V

I

A

Q

E

S

V

L

L

N

Q

V

-

S

-

L

L

P

A

H

H

-

L

L

L

D

D

A
x
R

V

K

V

P

A

K

L

A

M

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

S

V

V

V

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H
|
H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

binding adenosine-5'-diphosphate: W12 (≠ F24), G38 (= G50), C39 (≠ A51), G40 (= G52), K41 (= K53), S42 (= S54), T43 (= T55), R128 (≠ A154), S134 (= S160), Q137 (= Q163)
binding beryllium trifluoride ion: S37 (≠ N49), G38 (= G50), K41 (= K53), Q81 (= Q96), S134 (= S160), G136 (= G162), H191 (= H216)
binding magnesium ion: S42 (= S54), Q81 (= Q96)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
31% identity, 86% coverage: 15:235/257 of query aligns to 3:210/371 of 3puwA

query
sites
3puwA

L

V

S

Q

V

L

S

Q

G

N

A

V

C

T

K

K

R

A

F
x
W

G

G

S

E

V

V

A
x
V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

E

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

K

N

R

V

M

L

N

R

D

S

T

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R

K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

I

D

N

E

Q

Q

R

T

V

R

N

S

Q

L

V

I

A

Q

E

S

V

L

L

N

Q

V

-

S

-

L

L

P

A

H

H

-

L

L

L

D

D

A
x
R

V

K

V

P

A

K

L
x
A

M

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

S

V

V

V

F

I

L

L

L

D

D

E
|
E

P

P

T

L

A

S

A

N

L

L

G

D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H
|
H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

binding adenosine-5'-diphosphate: W12 (≠ F24), V17 (≠ A29), G38 (= G50), C39 (≠ A51), G40 (= G52), K41 (= K53), S42 (= S54), T43 (= T55), R128 (≠ A154), A132 (≠ L158), S134 (= S160), Q137 (= Q163)
binding tetrafluoroaluminate ion: S37 (≠ N49), G38 (= G50), K41 (= K53), Q81 (= Q96), S134 (= S160), G135 (= G161), G136 (= G162), E158 (= E184), H191 (= H216)
binding magnesium ion: S42 (= S54), Q81 (= Q96)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
31% identity, 86% coverage: 15:235/257 of query aligns to 3:210/371 of 3puvA

query
sites
3puvA

L

V

S

Q

V

L

S

Q

G

N

A

V

C

T

K

K

R

A

F
x
W

G

G

S

E

V

V

A
x
V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

E

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

K

N

R

V

M

L

N

R

D

S

T

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R

K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

I

D

N

E

Q

Q

R

T

V

R

N

S

Q

L

V

I

A

Q

E

S

V

L

L

N

Q

V

-

S

-

L

L

P

A

H

H

-

L

L

L

D

D

A
x
R

V

K

V

P

A

K

L
x
A

M

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

S

V

V

V

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H

H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

binding adenosine-5'-diphosphate: W12 (≠ F24), V17 (≠ A29), G38 (= G50), C39 (≠ A51), G40 (= G52), K41 (= K53), S42 (= S54), T43 (= T55), R128 (≠ A154), A132 (≠ L158), S134 (= S160), Q137 (= Q163)
binding magnesium ion: S42 (= S54), Q81 (= Q96)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 86% coverage: 15:235/257 of query aligns to 4:211/371 of P68187

query
sites
P68187

L

V

S

Q

V

L

S

Q

G

N

A

V

C

T

K

K

R

A

F

W

G

G

S

E

V

V

A

V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

E

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

K

N

R

V

M

L

N

R

D

S

T

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q

Q

D

S

L

Y

A
|
A

L

L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R
x
K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

I

D

N

E

Q

Q

R

T
x
V

R

N

S

Q

L
x
V

I

A

Q
x
E

S

V

L

L

N

Q

V

-

S

-

L

L

P
x
A

H

H

-

L

L

L

D

D

A

R

V

K

V

P

A

K

L

A

M

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

S

V

V

V

F

I

L

L

L

D
|
D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H

H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

A85 (= A99) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ R120) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ T138) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L141) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ Q143) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ P150) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G162) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D183) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
31% identity, 86% coverage: 15:235/257 of query aligns to 1:208/367 of 1q12A

query
sites
1q12A

L

V

S

Q

V

L

S

Q

G

N

A

V

C

T

K

K

R

A

F
x
W

G

G

S

E

V

V

A

V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

E

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

K

N

R

V

M

L

N

R

D

S

T

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q

Q

D

S

L

Y

A

A

L

L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R

K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

I

D

N

E

Q

Q

R

T

V

R

N

S

Q

L

V

I

A

Q

E

S

V

L

L

N

Q

V

-

S

-

L

L

P

A

H

H

-

L

L

L

D

D

A
x
R

V

K

V

P

A

K

L
x
A

M

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

S

V

V

V

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H

H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

binding adenosine-5'-triphosphate: W10 (≠ F24), S35 (≠ N49), G36 (= G50), C37 (≠ A51), G38 (= G52), K39 (= K53), S40 (= S54), T41 (= T55), R126 (≠ A154), A130 (≠ L158), S132 (= S160), G134 (= G162), Q135 (= Q163)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
31% identity, 86% coverage: 15:235/257 of query aligns to 3:210/374 of 2awnB

query
sites
2awnB

L

V

S

Q

V

L

S

Q

G

N

A

V

C

T

K

K

R

A

F
x
W

G

G

S

E

V

V

A
x
V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

E

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

K

N

R

V

M

L

N

R

D

S

T

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q

Q

D

S

L

Y

A

A

L

L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R

K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

I

D

N

E

Q

Q

R

T

V

R

N

S

Q

L

V

I

A

Q

E

S

V

L

L

N

Q

V

-

S

-

L

L

P

A

H

H

-

L

L

L

D

D

A

R

V

K

V

P

A

K

L

A

M

L

S

S

G

G

Q

Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

S

V

V

V

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H

H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

binding adenosine-5'-diphosphate: W12 (≠ F24), V17 (≠ A29), S37 (≠ N49), G38 (= G50), G40 (= G52), K41 (= K53), S42 (= S54), T43 (= T55)

7mdyC Lolcde nucleotide-bound
33% identity, 88% coverage: 14:240/257 of query aligns to 2:224/226 of 7mdyC

query
sites
7mdyC

V

L

L

L

S

Q

V

C

S

D

G

N

A

L

C

C

K

K

R

R

F
x
Y

-

Q

-

E

G

G

S

S

V

V

V

Q

A

T

-

D

-

V

L

L

D

H

N

N

V

V

S

S

I

F

D

S

V

V

H

G

R

E

G

G

E

E

I

M

L

M

A

A

L

I

L

V

G

G

D

S

N

S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

H

C

L

I

L

S

G

G

G

V

L

H

D

R

T

L

P

D

T

A

S

G

G

D

D

V

V

V

I

I

F

D

N

G

G

A

Q

A

P

-

M

N

S

V

K

L

L

R

S

S

S

P

A

A

A

D

K

A

A

R

E

L

L

A

R

G

N

-

Q

-

K

I

L

E

G

T

F

V

I

Y

Y

Q
|
Q

D

-

L

-

A

-

L

-

F

F

D

H

N

H

L

L

T

L

P

P

A

-

Q

-

N

D

F

F

F

T

A

A

G

L

R

E

E

N

I

V

A

A

G

M

P

P

R

L

W

L

F

I

P

G

R

K

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

K

K

P

M

A

D

E

E

I

Q

N

T

S

R

R

S

A

L

L

I

E

Q

M

S

L

L

K

N

A

V

V

S

G

L

L

P

D

H

H

-

R

L

A

D

N

A
x
H

V

R

V

P

A

S

L
x
E

M

L

S
|
S

G
|
G

Q

E

R

R

Q

Q

A

R

I

V

A

A

V

I

A

A

R

R

S

A

A

L

V

V

F

N

A

N

R

P

K

R

V

L

V

V

I

L

L

A

D

D

E
|
E

P

P

T

T

A

G

A
x
N

L

L

G

D

Q

A

R

R

E

N

S

A

R

D

K

S

V

I

L

F

D

Q

L

L

I

L

G

G

A

E

L

L

-

N

R

R

D

L

R

Q

G

G

N

T

A

A

I

F

I

L

L

V

I

V

T

T

H
|
H

N

D

M

L

E

Q

H

L

V

A

T

K

E

R

L

M

A

S

C

-

R

R

A

Q

V

L

V

E

L

M

R

R

Q

D

G

G

R

R

R

L

V

T

G

A

E

E

L

L

binding adp orthovanadate: Y12 (≠ F24), G42 (= G50), S43 (≠ A51), G44 (= G52), K45 (= K53), S46 (= S54), T47 (= T55), Q91 (= Q96), H138 (≠ A154), E142 (≠ L158), S144 (= S160), G145 (= G161), G146 (= G162), E168 (= E184), N172 (≠ A188), H201 (= H216)
binding magnesium ion: S46 (= S54), Q91 (= Q96)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
33% identity, 88% coverage: 14:240/257 of query aligns to 5:227/233 of P75957

query
sites
P75957

V

L

L

L

S

Q

V

C

S

D

G

N

A

L

C

C

K

K

R

R

F

Y

-

Q

-

E

G

G

S

S

V

V

V

Q

A

T

-

D

-

V

L

L

D

H

N

N

V

V

S

S

I

F

D

S

V

V

H

G

R

E

G

G

E

E

I

M

L

M

A

A

L

I

L

V

G
|
G

D

S

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

I

L

K

H

C

L

I

L

S

G

G

G

V

L

H

D

R

T

L

P

D

T

A

S

G

G

D

D

V

V

V

I

I

F

D

N

G

G

A

Q

A

P

-

M

N

S

V

K

L

L

R

S

S

S

P

A

A

A

D

K

A

A

R

E

L

L

A

R

G

N

-

Q

-

K

I

L

E

G

T

F

V

I

Y

Y

Q

Q

D

-

L

-

A

-

L

-

F

F

D

H

N

H

L

L

T

L

P

P

A

-

Q

-

N

D

F

F

F

T

A

A

G

L

R

E

E

N

I

V

A

A

G

M

P

P

R

L

W

L

F

I

P

G

R

K

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

K

K

P

M

A

D

E

E

I

Q

N

T

S

R

R

S

A

L

L

I

E

Q

M

S

L

L

K

N

A

V

V

S

G

L

L

P

D

H

H

-

R

L

A

D

N

A

H

V

R

V

P

A

S

L

E

M

L

S

S

G

G

Q

E

R

R

Q

Q

A

R

I

V

A

A

V

I

A

A

R

R

S

A

A

L

V

V

F

N

A

N

R

P

K

R

V

L

V

V

I

L

L

A

D

D

E

E

P

P

T

T

A

G

A

N

L

L

G

D

Q

A

R

R

E

N

S

A

R

D

K

S

V

I

L

F

D

Q

L

L

I

L

G

G

A

E

L

L

-

N

R

R

D

L

R

Q

G

G

N

T

A

A

I

F

I

L

L

V

I

V

T

T

H

H

N

D

M

L

E

Q

H

L

V

A

T

K

E

R

L

M

A

S

C

-

R

R

A

Q

V

L

V

E

L

M

R

R

Q

D

G

G

R

R

R

L

V

T

G

A

E

E

L

L

G42 (= G47) mutation to D: Loss of lipoprotein release when overexpressed.

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 84% coverage: 21:235/257 of query aligns to 10:211/369 of P19566

query
sites
P19566

C

T

K

K

R

A

F

W

G

G

S

D

V

V

A

V

L

S

D

K

N

D

V

I

S

N

I

L

D

D

V

I

H

H

R

D

G

G

E

E

I

F

L

V

A

V

L

F

L

V

G

G

D

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

I

L

K

R

C

M

I

I

S

A

G

G

V

L

H

E

R

T

L

I

D

T

A

S

G

G

D

D

V

L

V

F

I

I

D

-

G

G

A

E

A

T

N

R

V

M

L

N

R

D

S

I

P

P

A

P

D

A

A

E

R

R

L

-

A

-

G

G

I

V

E

G

T

M

V

V

Y

F

Q

Q

D

S

L

Y

A

A

L
|
L

F

Y

D

P

N

H

L

L

T

S

P

V

A

A

Q

E

N

N

F

M

F

S

A

F

G

G

R

L

E

K

I

L

A

A

G

G

P

A

R

K

W

-

F

-

P

-

R

-

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

E

K

V

M

M

D

N

E

Q

Q

R

T

V

R

N

S

Q

L

V

I

A

Q

E

S

V

L

L

N

Q

V

-

S

-

L

L

P

A

H

H

-

L

L

L

D

E

A

R

V

K

V

P

A

K

L

A

M

L

S

S

G

G

Q

Q

R

R

Q

Q

A

R

I

V

A

A

V

I

A

G

R

R

S

T

A

L

V

V

F

A

A

E

R

P

K

R

V

V

V

F

I

L

L

L

D

D

E

E

P
|
P

T

L

A

S

A

N

L

L

G
x
D

Q

A

R

A

E

L

S

R

R

V

K

Q

V

M

L

R

D

I

L

E

I

I

G

S

A

R

L

L

R

H

D

K

R

R

-

L

G

G

N

R

A

T

I

M

I

I

L

Y

I

V

T

T

H

H

N

D

M

Q

E

V

H

E

V

A

T

M

E

T

L

L

A

A

C

D

R

K

A

I

V

V

L

L

R

D

Q

A

G

G

R

R

L86 (= L100) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P185) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (≠ G190) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
26% identity, 88% coverage: 14:239/257 of query aligns to 4:231/253 of 1g9xB

query
sites
1g9xB

V

I

L

L

S

R

V

T

S

E

G

N

A

I

C

V

K

K

R

Y

F
|
F

G

G

S

E

V
x
F

V

K

A

A

L

L

D

D

N

G

V

V

S

S

I

I

D

S

V

V

H

C

R

K

G

G

E

D

I

V

L

T

A

L

L

I

G

G

D

P

N

N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

N

C

V

I

I

S

T

G

G

V

F

H

L

R

K

L

A

D

D

A

E

G

G

D

R

V

V

V

Y

I

F

D

E

G

N

A

K

A

D

N

I

V

T

L

N

R

K

S

E

P

P

A

A

D

E

A

L

R

Y

L

H

A

Y

G

G

I

I

E

V

T

R

V

T

Y

F

Q

Q

D

T

L

P

A

Q

L

P

F

L

D

K

N

E

L

M

T

T

P

V

A

L

Q

E

N

N

F

L

A

I

G

G

R

E

E

I

I

N

A

P

G

G

-

E

-

S

-

P

-

L

-

N

-

S

-

L

-

F

-

Y

P

K

R

K

W

W

F

I

P

P

R

K

A

-

M

-

R

-

F

-

L

-

N

-

K

E

R

E

K

E

M

M

D

V

E

E

Q

K

T

A

R

F

S

K

L

I

I

L

Q

E

S

F

L

L

N

K

V

L

S

S

L

-

P

-

H

H

L

L

-

Y

D

D

A

R

V

K

V

A

A

G

L

E

M

L

S

S

G

G

Q

Q

R

M

Q

K

A

L

I

V

A

E

V

I

A

G

R

R

S

A

A

L

V

M

F

T

A

N

R

P

K

K

V

M

V

I

I

V

L

M

D

D

E
|
E

P

P

T

I

A

A

A

G

L

V

G

A

Q

P

R

G

E

L

S

A

R

H

K

D

V

I

L

F

D

N

L

H

I

V

G

L

A

E

L

L

R

K

D

A

R

K

G

G

N

I

A

T

I

F

I

L

L

I

I

I

T

E

H

H

N

R

M

L

E

D

H

I

V

V

T

L

E

N

L

Y

A

I

C

D

R

H

A

L

V

Y

V

V

L

M

R

F

Q

N

G

G

R

Q

R

I

V

I

G

A

E

E

binding adenosine-5'-diphosphate: F14 (= F24), F17 (≠ V27), G40 (= G50), S41 (≠ A51), G42 (= G52), K43 (= K53), S44 (= S54), T45 (= T55)
binding magnesium ion: S44 (= S54), E176 (= E184)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
32% identity, 88% coverage: 14:240/257 of query aligns to 4:226/229 of 7v8iD

query
sites
7v8iD

V

L

L

L

S

Q

V

C

S

D

G

N

A

L

C

C

K

K

R

R

F

Y

-

Q

-

E

G

G

S

S

V

V

V

Q

A

T

-

D

-
x
V

L

L

D

H

N

N

V

V

S

S

I

F

D

S

V

V

H

G

R

E

G

G

E

E

I

M

L

M

A

A

L

I

L

V

G

G

D

S

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

H

C

L

I

L

S

G

G

G

V

L

H

D

R

T

L

P

D

T

A

S

G

G

D

D

V

V

V

I

I

F

D

N

G

G

A

Q

A

P

-

M

N

S

V

K

L

L

R

S

S

S

P

A

A

A

D

K

A

A

R

E

L

L

A

R

G

N

-

Q

-

K

I

L

E

G

T

F

V

I

Y

Y

Q
|
Q

D

-

L

-

A

-

L

-

F

F

D

H

N

H

L

L

T

L

P

P

A

-

Q

-

N

D

F

F

F

T

A

A

G

L

R

E

E

N

I

V

A

A

G

M

P

P

R

L

W

L

F

I

P

G

R

K

A

-

M

-

R

-

F

-

L

-

N

-

K

K

R

K

K

P

M

A

D

E

E

I

Q

N

T

S

R

R

S

A

L

L

I

E

Q

M

S

L

L

K

N

A

V

V

S

G

L

L

P

D

H

H

-
x
R

L

A

D

N

A
x
H

V

R

V

P

A

S

L
x
E

M

L

S
|
S

G

G

G
|
G

Q
x
E

R

R

Q

Q

A

R

I

V

A

A

V

I

A

A

R

R

S

A

A

L

V

V

F

N

A

N

R

P

K

R

V

L

V

V

I

L

L

A

D

D

E

Q

P

P

T

T

A

G

A

N

L

L

G

D

Q

A

R

R

E

N

S

A

R

D

K

S

V

I

L

F

D

Q

L

L

I

L

G

G

A

E

L

L

-

N

R

R

D

L

R

Q

G

G

N

T

A

A

I

F

I

L

L

V

I

V

T

T

H
|
H

N

D

M

L

E

Q

H

L

V

A

T

K

E

R

L

M

A

S

C

-

R

R

A

Q

V

L

V

E

L

M

R

R

Q

D

G

G

R

R

R

L

V

T

G

A

E

E

L

L

binding phosphoaminophosphonic acid-adenylate ester: V23 (vs. gap), S43 (≠ N49), G44 (= G50), G46 (= G52), K47 (= K53), S48 (= S54), T49 (= T55), Q93 (= Q96), R137 (vs. gap), H140 (≠ A154), E144 (≠ L158), S146 (= S160), G148 (= G162), E149 (≠ Q163), H203 (= H216)
binding magnesium ion: S48 (= S54), Q93 (= Q96)

3c4jA Abc protein artp in complex with atp-gamma-s
27% identity, 89% coverage: 14:242/257 of query aligns to 3:222/242 of 3c4jA

query
sites
3c4jA

V

M

L

I

S

D

V

V

S

H

G

Q

A

L

C

K

K

K

R

S

F
|
F

G

G

S

S

V

L

V

E

A
x
V

L

L

D

K

N

G

V

I

S

N

I

V

D

H

V

I

H

R

R

E

G

G

E

E

I

V

L

V

A

V

L

V

L

I

G

G

D

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

C

C

I

L

S

N

G

L

V

L

H

E

R

D

L

F

D

D

A

E

G

G

D

E

V

I

I

I

D

D

G

G

A

-

N

-

V

I

L

N

R

L

S

K

P

A

A

K

D

D

A

T

R

N

L

L

A

N

G

K

I

V

E

R

-

E

-

V

-

G

T

M

V

V

Y

F

Q

Q

D

R

L

F

A

N

L

L

F

F

D

P

N

H

L

M

T

T

P

V

A

L

Q

N

N

N

F

I

-

T

F

L

A

A

G

P

R

M

E

K

I

V

A

-

G

-

P

-

R

R

W

K

F

W

P

P

R

R

-

E

-

K

-

A

-

E

-

A

-

K

A

A

M

M

R

E

F

L

L

L

N

D

K

K

R

V

K

G

M

L

D

K

E

D

Q

K

T

A

R

H

S

A

L

Y

I

P

Q

D

S

S

L

L

N

-

V

-

S

-

L

-

P

-

H

-

L

-

D

-

A

-

V

-

A

-

L

-

M

-

S

S

G

G

Q

Q

R

A

Q

Q

A

R

I

V

A

A

V

I

A

A

R

R

S

A

A

L

V

A

F

M

A

E

R

P

K

K

V

I

V

M

I

L

L

F

D

D

E

E

P

P

T

T

A

S

A

A

L

L

G

D

Q

P

R

E

E

M

S

V

R

G

K

E

V

V

L

L

D

S

L

V

I

M

G

K

A

Q

L

L

R

A

D

N

R

E

G

G

N

M

A

T

I

M

I

V

L

V

I

V

T

T

H

H

N

E

M

M

E

G

H

F

V

A

T

R

E

E

L

V

A

G

C

D

R

R

A

V

V

L

V

F

L

M

R

D

Q

G

G

G

R

Y

R

I

V

I

G

E

E

E

L

G

V

K

P

P

binding phosphothiophosphoric acid-adenylate ester: F13 (= F24), V18 (≠ A29), S38 (≠ N49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55)

3c41J Abc protein artp in complex with amp-pnp/mg2+
27% identity, 89% coverage: 14:242/257 of query aligns to 3:222/242 of 3c41J

query
sites
3c41J

V

M

L

I

S

D

V

V

S

H

G

Q

A

L

C

K

K

K

R

S

F
|
F

G

G

S

S

V

L

V

E

A
x
V

L

L

D

K

N

G

V

I

S

N

I

V

D

H

V

I

H

R

R

E

G

G

E

E

I

V

L

V

A

V

L

V

L

I

G

G

D

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

C

C

I

L

S

N

G

L

V

L

H

E

R

D

L

F

D

D

A

E

G

G

D

E

V

I

I

I

D

D

G

G

A

-

N

-

V

I

L

N

R

L

S

K

P

A

A

K

D

D

A

T

R

N

L

L

A

N

G

K

I

V

E

R

-

E

-

V

-

G

T

M

V

V

Y

F

Q

Q

D

R

L

F

A

N

L

L

F

F

D

P

N

H

L

M

T

T

P

V

A

L

Q

N

N

N

F

I

-

T

F

L

A

A

G

P

R

M

E

K

I

V

A

-

G

-

P

-

R

R

W

K

F

W

P

P

R

R

-

E

-

K

-

A

-

E

-

A

-

K

A

A

M

M

R

E

F

L

L

L

N

D

K

K

R

V

K

G

M

L

D

K

E

D

Q

K

T

A

R

H

S

A

L

Y

I

P

Q

D

S

S

L

L

N

-

V

-

S

-

L

-

P

-

H

-

L

-

D

-

A

-

V

-

A

-

L

-

M

-

S

S

G

G

Q

Q

R

A

Q

Q

A

R

I

V

A

A

V

I

A

A

R

R

S

A

A

L

V

A

F

M

A

E

R

P

K

K

V

I

V

M

I

L

L

F

D
|
D

E

E

P

P

T

T

A

S

A

A

L

L

G

D

Q

P

R

E

E

M

S

V

R

G

K

E

V

V

L

L

D

S

L

V

I

M

G

K

A

Q

L

L

R

A

D

N

R

E

G

G

N

M

A

T

I

M

I

V

L

V

I

V

T

T

H

H

N

E

M

M

E

G

H

F

V

A

T

R

E

E

L

V

A

G

C

D

R

R

A

V

V

L

V

F

L

M

R

D

Q

G

G

G

R

Y

R

I

V

I

G

E

E

E

L

G

V

K

P

P

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F24), V18 (≠ A29), S38 (≠ N49), G39 (= G50), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55)
binding magnesium ion: S43 (= S54), D163 (= D183)

2olkA Abc protein artp in complex with adp-beta-s
27% identity, 89% coverage: 14:242/257 of query aligns to 3:222/242 of 2olkA

query
sites
2olkA

V

M

L

I

S

D

V

V

S

H

G

Q

A

L

C

K

K

K

R

S

F
|
F

G

G

S

S

V

L

V

E