SitesBLAST
Comparing WP_108358556.1 NCBI__GCF_003063475.1:WP_108358556.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6aonA 1.72 angstrom resolution crystal structure of 2-oxoglutarate dehydrogenase complex subunit dihydrolipoamide dehydrogenase from bordetella pertussis in complex with fad
75% identity, 100% coverage: 3:475/475 of query aligns to 1:473/473 of 6aonA
- active site: P43 (= P45), C47 (= C49), C52 (= C54), S55 (= S57), V191 (= V193), E195 (= E197), H450 (= H452), H452 (= H454), E457 (= E459)
- binding calcium ion: A218 (≠ G220), A220 (≠ V222), Q222 (≠ E224)
- binding flavin-adenine dinucleotide: I8 (= I10), G11 (= G13), P12 (= P14), G13 (= G15), D32 (= D34), A33 (≠ E35), W34 (= W36), G45 (= G47), T46 (= T48), C47 (= C49), G51 (= G53), C52 (= C54), K56 (= K58), K119 (≠ R121), G120 (= G122), T151 (= T153), G152 (= G154), N171 (= N173), I192 (= I194), R280 (= R282), Y283 (≠ N285), G319 (= G321), D320 (= D322), M326 (= M328), L327 (= L329), A328 (= A330), H329 (= H331)
P14218 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex; EC 1.8.1.4 from Pseudomonas fluorescens (see 2 papers)
56% identity, 100% coverage: 1:475/475 of query aligns to 1:472/478 of P14218
- M1 (= M1) modified: Initiator methionine, Removed
- 34:49 (vs. 34:49, 38% identical) binding FAD
- C49 (= C49) modified: Disulfide link with 54, Redox-active
- C54 (= C54) modified: Disulfide link with 49, Redox-active
- K58 (= K58) binding FAD
- G122 (= G122) binding FAD
- D319 (= D322) binding FAD
- A327 (= A330) binding FAD
5u8uD Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa (see paper)
56% identity, 100% coverage: 1:475/475 of query aligns to 3:474/477 of 5u8uD
- active site: P16 (= P14), L47 (≠ P45), C51 (= C49), C56 (= C54), S59 (= S57), G85 (≠ D83), V86 (= V84), V193 (= V193), E197 (= E197), S333 (≠ E334), F451 (≠ H452), H453 (= H454), E458 (= E459)
- binding flavin-adenine dinucleotide: I12 (= I10), G15 (= G13), P16 (= P14), G17 (= G15), E36 (≠ D34), K37 (≠ E35), G49 (= G47), T50 (= T48), C51 (= C49), G55 (= G53), C56 (= C54), K60 (= K58), H123 (≠ R121), G124 (= G122), A152 (= A152), S153 (≠ T153), G154 (= G154), I194 (= I194), R281 (= R282), G320 (= G321), D321 (= D322), M327 (= M328), L328 (= L329), A329 (= A330), H330 (= H331), H453 (= H454), P454 (= P455)
Sites not aligning to the query:
5u8wA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to nadh (see paper)
56% identity, 100% coverage: 2:475/475 of query aligns to 1:471/473 of 5u8wA
- active site: P13 (= P14), L44 (≠ P45), C48 (= C49), C53 (= C54), S56 (= S57), G82 (≠ D83), V83 (= V84), V190 (= V193), E194 (= E197), S330 (≠ E334), F448 (≠ H452), H450 (= H454), E455 (= E459)
- binding flavin-adenine dinucleotide: I9 (= I10), G12 (= G13), P13 (= P14), G14 (= G15), E33 (≠ D34), K34 (≠ E35), G46 (= G47), T47 (= T48), C48 (= C49), G52 (= G53), C53 (= C54), K57 (= K58), H120 (≠ R121), G121 (= G122), A149 (= A152), S150 (≠ T153), G151 (= G154), S170 (≠ N173), G317 (= G321), D318 (= D322), M324 (= M328), L325 (= L329), A326 (= A330), H327 (= H331), Y357 (= Y361), H450 (= H454), P451 (= P455)
- binding 1,4-dihydronicotinamide adenine dinucleotide: I186 (= I189), G189 (= G192), V190 (= V193), I191 (= I194), E194 (= E197), E210 (= E213), A211 (≠ G214), L212 (= L215), A275 (≠ S279), V276 (≠ I280), G277 (= G281), R278 (= R282), M324 (= M328), L325 (= L329), V355 (= V359), Y357 (= Y361)
Sites not aligning to the query:
5u8vA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to NAD+ (see paper)
56% identity, 100% coverage: 3:475/475 of query aligns to 1:470/472 of 5u8vA
- active site: P12 (= P14), L43 (≠ P45), C47 (= C49), C52 (= C54), S55 (= S57), G81 (≠ D83), V82 (= V84), V189 (= V193), E193 (= E197), S329 (≠ E334), F447 (≠ H452), H449 (= H454), E454 (= E459)
- binding flavin-adenine dinucleotide: I8 (= I10), G11 (= G13), P12 (= P14), G13 (= G15), E32 (≠ D34), G45 (= G47), T46 (= T48), C47 (= C49), G51 (= G53), C52 (= C54), K56 (= K58), H119 (≠ R121), G120 (= G122), A148 (= A152), S149 (≠ T153), G150 (= G154), S169 (≠ N173), I190 (= I194), R277 (= R282), G316 (= G321), D317 (= D322), M323 (= M328), L324 (= L329), A325 (= A330), H326 (= H331), H449 (= H454), P450 (= P455)
- binding nicotinamide-adenine-dinucleotide: I185 (= I189), G186 (= G190), G188 (= G192), V189 (= V193), I190 (= I194), L208 (= L212), E209 (= E213), A210 (≠ G214), V243 (= V247), V275 (≠ I280), G276 (= G281)
Sites not aligning to the query:
6awaA 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from pseudomonas putida in complex with fad and adenosine-5'-monophosphate.
55% identity, 99% coverage: 1:469/475 of query aligns to 1:466/475 of 6awaA
- active site: L45 (≠ P45), C49 (= C49), C54 (= C54), S57 (= S57), V191 (= V193), E195 (= E197), F449 (≠ H452), H451 (= H454), E456 (= E459)
- binding adenosine monophosphate: I187 (= I189), E211 (= E213), A212 (≠ G214), L213 (= L215), V245 (= V247), V277 (≠ I280)
- binding flavin-adenine dinucleotide: I10 (= I10), G13 (= G13), P14 (= P14), G15 (= G15), E34 (≠ D34), K35 (≠ E35), T48 (= T48), C49 (= C49), G53 (= G53), C54 (= C54), K58 (= K58), H121 (≠ R121), G122 (= G122), S151 (≠ T153), G152 (= G154), I192 (= I194), R279 (= R282), G318 (= G321), D319 (= D322), M325 (= M328), L326 (= L329), A327 (= A330), Y358 (= Y361)
Sites not aligning to the query:
6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
53% identity, 99% coverage: 4:475/475 of query aligns to 6:470/470 of 6uziC
- active site: C45 (= C49), C50 (= C54), S53 (= S57), V187 (= V193), E191 (= E197), H448 (= H454), E453 (= E459)
- binding flavin-adenine dinucleotide: I12 (= I10), G13 (= G11), G15 (= G13), P16 (= P14), G17 (= G15), E36 (≠ D34), K37 (≠ E35), G43 (= G47), T44 (= T48), C45 (= C49), G49 (= G53), C50 (= C54), S53 (= S57), K54 (= K58), V117 (≠ R121), G118 (= G122), T147 (= T153), G148 (= G154), I188 (= I194), R276 (= R282), D316 (= D322), M322 (= M328), L323 (= L329), A324 (= A330)
- binding zinc ion: H448 (= H454), E453 (= E459)
P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
54% identity, 100% coverage: 1:474/475 of query aligns to 1:471/477 of P18925
- 34:49 (vs. 34:49, 44% identical) binding FAD
- C49 (= C49) modified: Disulfide link with 54, Redox-active
- C54 (= C54) modified: Disulfide link with 49, Redox-active
- K58 (= K58) binding FAD
- D319 (= D322) binding FAD
- A327 (= A330) binding FAD
3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
54% identity, 100% coverage: 2:474/475 of query aligns to 1:470/472 of 3ladA