SitesBLAST
Comparing WP_108401501.1 NCBI__GCF_003063475.1:WP_108401501.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5wm3A Crystal structure of cahj in complex with salicyl adenylate (see paper)
32% identity, 95% coverage: 17:538/547 of query aligns to 20:530/537 of 5wm3A
- active site: S193 (≠ T205), N213 (= N225), H237 (= H249), A336 (≠ T347), E337 (= E348), N437 (≠ I446), K442 (≠ N451), K524 (= K532)
- binding 9-(5-O-{(S)-hydroxy[(2-hydroxybenzene-1-carbonyl)oxy]phosphoryl}-alpha-L-lyxofuranosyl)-9H-purin-6-amine: N238 (≠ Q250), F239 (≠ T251), G310 (= G321), S311 (≠ A322), K312 (≠ P323), V332 (≠ A343), F333 (≠ W344), G334 (= G345), M335 (= M346), A336 (≠ T347), D416 (= D425), K433 (= K442), K442 (≠ N451)
- binding magnesium ion: S301 (≠ P312), L303 (= L314), G326 (= G337)
5wm2A Crystal structure of cahj in complex with salicylic acid and amp (see paper)
32% identity, 95% coverage: 17:538/547 of query aligns to 20:530/536 of 5wm2A
- active site: S193 (≠ T205), N213 (= N225), H237 (= H249), A336 (≠ T347), E337 (= E348), N437 (≠ I446), K442 (≠ N451), K524 (= K532)
- binding adenosine monophosphate: G310 (= G321), S311 (≠ A322), K312 (≠ P323), V332 (≠ A343), F333 (≠ W344), G334 (= G345), M335 (= M346), A336 (≠ T347), E337 (= E348), D416 (= D425), V428 (≠ I437), K433 (= K442), K442 (≠ N451)
5wm6A Crystal structure of cahj in complex with benzoyl adenylate (see paper)
32% identity, 95% coverage: 17:538/547 of query aligns to 20:530/535 of 5wm6A
- active site: S193 (≠ T205), N213 (= N225), H237 (= H249), A336 (≠ T347), E337 (= E348), N437 (≠ I446), K442 (≠ N451), K524 (= K532)
- binding magnesium ion: S301 (≠ P312), L303 (= L314), G326 (= G337)
- binding 5'-O-[(R)-(benzoyloxy)(hydroxy)phosphoryl]adenosine: F239 (≠ T251), G310 (= G321), S311 (≠ A322), K312 (≠ P323), V332 (≠ A343), F333 (≠ W344), G334 (= G345), M335 (= M346), A336 (≠ T347), D416 (= D425), K433 (= K442), K442 (≠ N451)
5wm5A Crystal structure of cahj in complex with 5-methylsalicyl adenylate (see paper)
32% identity, 95% coverage: 17:538/547 of query aligns to 20:528/533 of 5wm5A
- active site: S193 (≠ T205), N213 (= N225), H237 (= H249), A336 (≠ T347), E337 (= E348), N437 (≠ I446), K442 (≠ N451), K522 (= K532)
- binding 9-(5-O-{(S)-hydroxy[(2-hydroxy-5-methylbenzene-1-carbonyl)oxy]phosphoryl}-alpha-L-lyxofuranosyl)-9H-purin-6-amine: H237 (= H249), N238 (≠ Q250), F239 (≠ T251), G309 (≠ A320), G310 (= G321), S311 (≠ A322), K312 (≠ P323), V332 (≠ A343), F333 (≠ W344), G334 (= G345), M335 (= M346), A336 (≠ T347), L340 (≠ V352), D416 (= D425), K433 (= K442), K442 (≠ N451)
5wm4A Crystal structure of cahj in complex with 6-methylsalicyl adenylate (see paper)
32% identity, 94% coverage: 17:532/547 of query aligns to 20:524/534 of 5wm4A
- active site: S193 (≠ T205), N213 (= N225), H237 (= H249), A336 (≠ T347), E337 (= E348), N437 (≠ I446), K442 (≠ N451), K521 (≠ P529)
- binding 9-(5-O-{(S)-hydroxy[(2-hydroxy-6-methylbenzene-1-carbonyl)oxy]phosphoryl}-alpha-L-lyxofuranosyl)-9H-purin-6-amine: N238 (≠ Q250), F239 (≠ T251), C243 (≠ Y255), G309 (≠ A320), G310 (= G321), S311 (≠ A322), K312 (≠ P323), V332 (≠ A343), F333 (≠ W344), G334 (= G345), M335 (= M346), A336 (≠ T347), L340 (≠ V352), D416 (= D425), K433 (= K442), K442 (≠ N451)
- binding magnesium ion: M453 (≠ Y462), H455 (= H464), V458 (≠ I467)
P40871 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; EC 6.2.1.71 from Bacillus subtilis (strain 168) (see paper)
30% identity, 96% coverage: 19:545/547 of query aligns to 20:532/539 of P40871
- G191 (≠ S206) binding ATP
- HN 234:235 (≠ HQ 249:250) binding substrate
- S240 (≠ Y255) binding substrate
- G307 (= G321) binding ATP
- V329 (≠ A343) binding ATP
- D413 (= D425) binding ATP
- R428 (= R440) binding ATP
- K519 (= K532) binding ATP; binding substrate
1md9A Crystal structure of dhbe in complex with dhb and amp (see paper)
30% identity, 96% coverage: 19:545/547 of query aligns to 20:532/536 of 1md9A
- active site: S190 (≠ T205), S210 (≠ N225), H234 (= H249), A333 (≠ T347), E334 (= E348), N434 (≠ I446), K439 (≠ N451), K519 (= K532)
- binding adenosine monophosphate: G191 (≠ S206), G307 (= G321), A308 (= A322), K309 (≠ P323), V329 (≠ A343), F330 (≠ W344), G331 (= G345), M332 (= M346), D413 (= D425), V425 (≠ I437), R428 (= R440), K519 (= K532)
- binding 2,3-dihydroxy-benzoic acid: H234 (= H249), N235 (≠ Q250), Y236 (≠ T251), S240 (≠ Y255), G307 (= G321), V329 (≠ A343), G331 (= G345), M332 (= M346), K519 (= K532)
6e97B Crystal structure of the aryl acid adenylating enzyme fscc from fuscachelin nrps in complex with dhb-adenylate
31% identity, 96% coverage: 19:542/547 of query aligns to 17:532/537 of 6e97B
- active site: S190 (≠ T205), S210 (≠ N225), H234 (= H249), A336 (≠ T347), E337 (= E348), N437 (≠ I446), K442 (≠ N451), K522 (= K532)
- binding 5'-O-[(S)-[(2,3-dihydroxybenzene-1-carbonyl)oxy](hydroxy)phosphoryl]adenosine: H234 (= H249), N235 (≠ Q250), F236 (≠ T251), S240 (≠ Y255), G310 (= G321), A311 (= A322), K312 (≠ P323), V332 (≠ A343), F333 (≠ W344), G334 (= G345), M335 (= M346), A336 (≠ T347), D416 (= D425), K433 (= K442), K442 (≠ N451)
7tz4A Salicylate adenylate pchd from pseudomonas aeruginosa containing 4- cyanosalicyl-ams (see paper)
31% identity, 96% coverage: 19:545/547 of query aligns to 17:530/530 of 7tz4A