SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_108402015.1 NCBI__GCF_003063475.1:WP_108402015.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

5uhsA Structure of a semisweet d57a mutant (see paper)
45% identity, 82% coverage: 7:81/91 of query aligns to 5:79/81 of 5uhsA

query
sites
5uhsA

I

I

G

G

Y

Y

A

V

A

A

A

A

S

F

L

L

T

T

T

T

A

V

S

S

F

F

V

L

P

P

Q

Q

A

V

W

L

L

R

T

V

F

V

K

M

T

T

R

K

D

Q

V

T

S

R

G

D

I

I

S

S

L

R

G

N

M

M

Y

Y

S

I

A

M

F
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F

T

F

L

L

G

G

I

V

A

V

L

L

W
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W

L

F

A

V

Y

Y

G

G

L

I

L

L

I

R

E

S

A

A

W

L

P

P

V

I

V

I

I

L

A

A

N

N

I

V

I

V

T

T

L

L

V

F

L

F

A

V

A

T

S

I

I

I

L

L

A

Y

M

Y

R

K

L

L

binding beta-D-glucopyranose: F41 (= F43), W48 (= W50)

B0SR19 Sugar transporter SemiSWEET from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (see paper)
44% identity, 82% coverage: 7:81/91 of query aligns to 5:79/85 of B0SR19

query
sites
B0SR19

I

I

G

G

Y

Y

A

V

A

A

A

A

S

F

L

L

T

T

T

T

A

V

S

S

F

F

V

L

P

P

Q

Q

A

V

W

L

L

R

T

V

F

V

K

M

T

T

R

K

D

Q

V

T

S

R

G

D

I

I

S

S

L

R

G

N

M

M

Y

Y

S

I

A

M

F

F

T

F

L

L

G

G

I

V

A

V

L

L

W
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W

L

F

A

V

Y

Y

G

G

L

I

L

L

I

R

E

S

A

D

W

L

P

P

V

I

V

I

I

L

A

A

N
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N

I

V

I

V

T

T

L

L

V

F

L

F

A

V

A

T

S

I

I

I

L

L

A

Y

M

Y

R

K

L

L

W48 (= W50) mutation to A: Impairs glucose transport.
N64 (= N66) mutation to A: Impairs glucose transport.

P0DMV3 Sugar transporter SemiSWEET from Escherichia coli (strain UMEA 3162-1) (see paper)
43% identity, 76% coverage: 21:89/91 of query aligns to 21:89/89 of P0DMV3

query
sites
P0DMV3

P
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P

Q
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Q

A

S

W

I

L

K

T

T

F

I

K

R

T

T

R

R

D

N

V

T

S

E

G

G

I

I

S

S

L

V

G

V

M
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M

Y
|
Y

S

I

A

M

F

F

T

L

L

T

G

G

I

V

A

I

L

S

W
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W

L

I

A

A

Y
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Y

G

G

L

I

L

M

I
x
R

E

S

A

D

W

F

P

A

V

V

V

L

I

I

A

A

N
|
N

I

I

I

V

T

T

L

L

V

F

L

L

A

A

A

A

S

P

I

V

L

L

A

V

M

I

R

T

L

L

R

I

F

N

A

R

R

R

K

K

A

K

S

H

A

V

P21 (= P21) mutation to A: Strongly decreases sucrose transporter activity.
Q22 (= Q22) mutation to A: Slightly decreases sucrose transporter activity.
M39 (= M39) mutation to A: No effect on sucrose transporter activity.
Y40 (= Y40) mutation to A: Decreases sucrose transporter activity.
W50 (= W50) mutation to A: Increases sucrose transporter activity.; mutation to F: No effect on sucrose transporter activity.
Y53 (= Y53) mutation to F: Increases sucrose transporter activity.
R57 (≠ I57) mutation to A: Increases sucrose transporter activity.
N66 (= N66) mutation to A: Impairs sucrose transporter activity.

Sites not aligning to the query:

19 F→A: Decreases sucrose transporter activity.

Query Sequence

>WP_108402015.1 NCBI__GCF_003063475.1:WP_108402015.1
MNYTEWIGYAAASLTTASFVPQAWLTFKTRDVSGISLGMYSAFTLGIALWLAYGLLIEAW
PVVIANIITLVLAASILAMRLRFARKASAPN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory