SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_108402144.1 NCBI__GCF_003063475.1:WP_108402144.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 91% coverage: 19:472/500 of query aligns to 7:463/467 of 7qh2C

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binding flavin-adenine dinucleotide: V73 (≠ A85), G75 (= G87), S76 (≠ A88), G77 (= G89), T78 (= T90), G79 (= G91), L80 (= L92), A83 (≠ G95), C84 (≠ A96), P137 (= P149), G138 (≠ S150), E139 (≠ S151), A142 (≠ C155), T143 (= T156), G146 (= G159), N147 (= N160), S149 (≠ A162), T150 (≠ E163), A152 (≠ S165), G153 (= G166), E203 (= E215), G204 (= G216), I209 (≠ T221), E422 (= E431), H423 (= H432)
binding fe (iii) ion: H377 (= H387), H384 (= H394), E422 (= E431)

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 87% coverage: 39:473/500 of query aligns to 19:456/459 of P9WIT1

query
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P9WIT1

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K354 (≠ D366) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:456/456 of 8jdsA

query
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L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

N

D

D

D

P

A

D

E

Q

E

M

Q

H

R

R

R

C

V

E

K

Q

A

F

F

G

A

A

E

D

N

I

L

L

G

E

R

T

R

S

A

V

L

A

A

M

L

G

G

T

T

V

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

V

R

N

Q

S

L

M

L

C

Q

V

E

Q

E

F

V

S

G

A

P

E

V

E

G

N

V

E

E

Q

T

M

M

F

R

G

Q

V

L

K

K

R

N

A

T

F

L

D

D

S

P

K

R

G

G

L

L

L

M

N
|
N

P

P

G

G

K

K

V

V

I

L

binding flavin-adenine dinucleotide: P68 (≠ A85), G70 (= G87), T71 (≠ A88), G72 (= G89), T73 (= T90), G74 (= G91), G78 (= G95), V79 (≠ A96), L90 (= L107), P132 (= P149), G133 (≠ S150), A134 (≠ S151), G140 (= G159), M141 (≠ N160), A143 (= A162), T144 (≠ E163), A146 (≠ S165), S147 (≠ G166), E200 (= E215), G201 (= G216), I206 (≠ T221), W323 (≠ F342), E414 (= E431), H415 (= H432), N451 (= N468)
binding manganese (ii) ion: H370 (= H387), H377 (= H394), E414 (= E431)
binding pyruvic acid: R319 (= R338), H370 (= H387), H377 (= H394), H415 (= H432)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:455/455 of 8jdeA

query
sites
8jdeA

P

P

M

D

C

A

V

V

A

V

L

W

P

P

E

Q

T

N

V

V

D

D

Q

Q

V

V

Q

S

A

R

V

V

L

A

K

S

A

L

C

C

Y

Y

A

N

L

Q

D

G

V

V

P

P

V

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

M

C

P

A

H

V

T

Q

M

G

G

G

V

V

T

C

L

I

S

N

L
|
L

A

T

K

H

F

M

N

D

Q

Q

I

I

L

T

N

E

I

L

D

N

A

T

T

E

A

D

R

F

T

S

A

V

V

V

Q

E

C

P

G

G

V

V

R

T

N

R

L

K

A

A

I

L

S

N

E

T

A

H

V

L

S

R

Q

D

Y

S

G

G

L

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

I

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

V

T

H

N

C

A

L

V

K

R

Y

Y

G

G

L

T

T

M

V

R

H

D

N

N

V

V

L

I

K

N

V

L

K

E

G

V

F

V

T

L

I

P

E

D

G

G

E

R

P

L

I

L

E

H

F

T

G

A

S

G

D

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

T

A

P

A

G

G

L

Y

D

N

L

L

L

T

P

G

V

L

V

F

V

V

G

G

S

S

E
|
E

G
|
G

M

T

L

L

A

G

V

I

T
x
I

T

T

E

S

V

T

T

T

V

L

K

R

L

L

V

H

P

P

K

A

P

P

Q

E

L

A

A

T

R

V

C

A

I

A

M

T

A

C

S

A

F

F

D

P

D

S

V

V

R

Q

K

A

A

A

G

V

D

D

A

S

V

T

A

V

A

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

A

A

G

R

L

I

E

E

M

F

M

L

D

D

G

D

P

V

M

M

T

M

A

D

A

A

V

C

E

N

D

R

F

-

V

-

H

H

A

S

D

K

Y

L

D

N

L

C

S

P

A

V

A

A

A

P

I

T

L

L

L

F

C

L

E

E

S

F

D

H

G

G

T

S

P

Q

E

Q

E

T

V

L

E

A

E

E

E

Q

I

L

G

Q

R

R

M

T

S

E

E

A

V

I

L

T

R

Q

G

D

C

N

G

G

A

G

T

S

A

H

I

F

T

S

V

W

S

A

R

-

D

-

E

-

A

A

Q

E

R

K

M

N

K

E

F

L

W

W

S

A

G

A

R
|
R

K

H

N

N

A

A

F
x
W

P

Y

A

A

S

A

G

L

R

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

M

Y

C

S

M

T

D

D

S

V

T

C

I

V

P

P

R

I

K

S

R

R

L

L

A

P

D

E

I

I

L

L

E

V

A

E

I

T

S

K

E

E

M

E

E

I

K

K

K

A

Y

S

Q

K

L

L

R

T

C

G

L

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

N

D

D

D

P

A

D

E

Q

E

M

Q

H

R

R

R

C

V

E

K

Q

A

F

F

G

A

A

E

D

N

I

L

L

G

E

R

T

R

S

A

V

L

A

A

M

L

G

G

T

T

V

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

V

R

N

Q

S

L

M

L

C

Q

V

E

Q

E

F

V

S

G

A

P

E

V

E

G

N

V

E

E

Q

T

M

M

F

R

G

Q

V

L

K

K

R

N

A

T

F

L

D

D

S

P

K

R

G

G

L

L

L

M

N
|
N

P

P

G

G

K

K

V

V

I

L

binding flavin-adenine dinucleotide: P68 (≠ A85), G70 (= G87), T71 (≠ A88), G72 (= G89), T73 (= T90), G74 (= G91), G78 (= G95), V79 (≠ A96), L90 (= L107), P132 (= P149), G133 (≠ S150), A134 (≠ S151), G140 (= G159), M141 (≠ N160), A143 (= A162), T144 (≠ E163), A146 (≠ S165), S147 (≠ G166), E200 (= E215), G201 (= G216), I206 (≠ T221), W322 (≠ F342), E413 (= E431), H414 (= H432), N450 (= N468)
binding lactic acid: R318 (= R338), H369 (= H387), H376 (= H394), H414 (= H432)
binding manganese (ii) ion: H369 (= H387), H376 (= H394), E413 (= E431)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:455/455 of 8jdtA

query
sites
8jdtA

P

P

M

D

C

A

V

V

A

V

L

W

P

P

E

Q

T

N

V

V

D

D

Q

Q

V

V

Q

S

A

R

V

V

L

A

K

S

A

L

C

C

Y

Y

A

N

L

Q

D

G

V

V

P

P

V

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

M

C

P

A

H

V

T

Q

M

G

G

G

V

V

T

C

L

I

S

N

L
|
L

A

T

K

H

F

M

N

D

Q

Q

I

I

L

T

N

E

I

L

D

N

A

T

T

E

A

D

R

F

T

S

A

V

V

V

Q

E

C

P

G

G

V

V

R

T

N

R

L

K

A

A

I

L

S

N

E

T

A

H

V

L

S

R

Q

D

Y

S

G

G

L

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

I

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

V

T

H

N

C

A

L

V

K

R

Y

Y

G

G

L

T

T

M

V

R

H

D

N

N

V

V

L

I

K

N

V

L

K

E

G

V

F

V

T

L

I

P

E

D

G

G

E

R

P

L

I

L

E

H

F

T

G

A

S

G

D

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

T

A

P

A

G

G

L

Y

D

N

L

L

L

T

P

G

V

L

V

F

V

V

G

G

S

S

E
|
E

G
|
G

M

T

L

L

A

G

V

I

T
x
I

T

T

E

S

V

T

T

T

V

L

K

R

L

L

V

H

P

P

K

A

P

P

Q

E

L

A

A

T

R

V

C

A

I

A

M

T

A

C

S

A

F

F

D

P

D

S

V

V

R

Q

K

A

A

A

G

V

D

D

A

S

V

T

A

V

A

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

A

A

G

R

L

I

E

E

M

F

M

L

D

D

G

D

P

V

M

M

T

M

A

D

A

A

V

C

E

N

D

R

F

-

V

-

H

H

A

S

D

K

Y

L

D

N

L

C

S

P

A

V

A

A

A

P

I

T

L

L

L

F

C

L

E

E

S

F

D

H

G

G

T

S

P

Q

E

Q

E

T

V

L

E

A

E

E

E

Q

I

L

G

Q

R

R

M

T

S

E

E

A

V

I

L

T

R

Q

G

D

C

N

G

G

A

G

T

S

A

H

I

F

T

S

V

W

S

A

R

E

D

-

E

-

A

-

Q

K

R

R

M

N

K

E

F

L

W

W

S

A

G

A

R
|
R

K

H

N

N

A

A

F
x
W

P

Y

A

A

S

A

G

L

R

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

M

Y

C

S

M

T

D

D

S

V

T

C

I

V

P

P

R

I

K

S

R

R

L

L

A

P

D

E

I

I

L

L

E

V

A

E

I

T

S

K

E

E

M

E

E

I

K

K

K

A

Y

S

Q

K

L

L

R

T

C

G

L

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

N

D

D

D

P

A

D

E

Q

E

M

Q

H

R

R

R

C

V

E

K

Q

A

F

F

G

A

A

E

D

N

I

L

L

G

E

R

T

R

S

A

V

L

A

A

M

L

G

G

T

T

V

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

V

R

N

Q

S

L

M

L

C

Q

V

E

Q

E

F

V

S

G

A

P

E

V

E

G

N

V

E

E

Q

T

M

M

F

R

G

Q

V

L

K

K

R

N

A

T

F

L

D

D

S

P

K

R

G

G

L

L

L

M

N
|
N

P

P

G

G

K

K

V

V

I

L

binding 2-ketobutyric acid: R318 (= R338), H369 (= H387), H376 (= H394), H414 (= H432)
binding flavin-adenine dinucleotide: P68 (≠ A85), G70 (= G87), T71 (≠ A88), G72 (= G89), T73 (= T90), G74 (= G91), G78 (= G95), V79 (≠ A96), L90 (= L107), P132 (= P149), G133 (≠ S150), A134 (≠ S151), G140 (= G159), M141 (≠ N160), A143 (= A162), T144 (≠ E163), A146 (≠ S165), S147 (≠ G166), E200 (= E215), G201 (= G216), I206 (≠ T221), W322 (≠ F342), E413 (= E431), H414 (= H432), N450 (= N468)
binding manganese (ii) ion: H369 (= H387), H376 (= H394), E413 (= E431)

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:454/454 of 8jdvA

query
sites
8jdvA

P

P

M

D

C

A

V

V

A

V

L

W

P

P

E

Q

T

N

V

V

D

D

Q

Q

V

V

Q

S

A

R

V

V

L

A

K

S

A

L

C

C

Y

Y

A

N

L

Q

D

G

V

V

P

P

V

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L
x
V

S

E

G

G

G
|
G

A
x
V

M

C

P

A

H

V

T

Q

M

G

G

G

V

V

T

C

L

I

S

N

L
|
L

A

T

K

H

F

M

N

D

Q

Q

I

I

L

T

N

E

I

L

D

N

A

T

T

E

A

D

R

F

T

S

A

V

V

V

Q

E

C

P

G

G

V

V

R

T

N

R

L

K

A

A

I

L

S

N

E

T

A

H

V

L

S

R

Q

D

Y

S

G

G

L

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

I

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

V

T

H

N

C

A

L

V

K

R

Y

Y

G

G

L

T

T

M

V

R

H

D

N

N

V

V

L

I

K

N

V

L

K

E

G

V

F

V

T

L

I

P

E

D

G

G

E

R

P

L

I

L

E

H

F

T

G

A

S

G

D

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

T

A

P

A

G

G

L

Y

D

N

L

L

L

T

P

G

V

L

V

F

V

V

G

G

S

S

E
|
E

G
|
G

M

T

L

L

A

G

V

I

T
x
I

T

T

E

S

V

T

T

T

V

L

K

R

L

L

V

H

P

P

K

A

P

P

Q

E

L

A

A

T

R

V

C

A

I

A

M

T

A

C

S

A

F

F

D

P

D

S

V

V

R

Q

K

A

A

A

G

V

D

D

A

S

V

T

A

V

A

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

A

A

G

R

L

I

E

E

M

F

M

L

D

D

G

D

P

V

M

M

T

M

A

D

A

A

V

C

E

N

D

R

F

-

V

-

H

H

A

S

D

K

Y

L

D

N

L

C

S

P

A

V

A

A

A

P

I

T

L

L

L

F

C

L

E

E

S

F

D

H

G

G

T

S

P

Q

E

Q

E

T

V

L

E

A

E

E

E

Q

I

L

G

Q

R

R

M

T

S

E

E

A

V

I

L

T

R

Q

G

D

C

N

G

G

A

G

T

S

A

H

I

F

T

S

V

A

S

E

R

K

D

-

E

-

A

-

Q

-

R

R

M

N

K

E

F

L

W

W

S

A

G

A

R
|
R

K

H

N

N

A

A

F
x
W

P
x
Y

A

A

S

A

G

L

R

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

M

Y

C

S

M

T

D

D

S

V

T

C

I

V

P

P

R

I

K

S

R

R

L

L

A

P

D

E

I

I

L

L

E

V

A

E

I

T

S

K

E

E

M

E

E

I

K

K

K

A

Y

S

Q

K

L

L

R

T

C

G

L

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

N

D

D

D

P

A

D

E

Q

E

M

Q

H

R

R

R

C

V

E

K

Q

A

F

F

G

A

A

E

D

N

I

L

L

G

E

R

T

R

S

A

V

L

A

A

M

L

G

G

T

T

V

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

V

R

N

Q

S

L

M

L

C

Q

V

E

Q

E

F

V

S

G

A

P

E

V

E

G

N

V

E

E

Q

T

M

M

F

R

G

Q

V

L

K

K

R

N

A

T

F

L

D

D

S

P

K

R

G

G

L

L

L

M

N
|
N

P

P

G

G

K

K

V

V

I

L

binding 2-Ketohexanoic acid: V75 (≠ L92), R317 (= R338), W321 (≠ F342), H368 (= H387), H375 (= H394), H413 (= H432)
binding flavin-adenine dinucleotide: P68 (≠ A85), G70 (= G87), T71 (≠ A88), G72 (= G89), T73 (= T90), G74 (= G91), G78 (= G95), V79 (≠ A96), L90 (= L107), P132 (= P149), G133 (≠ S150), A134 (≠ S151), G140 (= G159), M141 (≠ N160), A143 (= A162), T144 (≠ E163), A146 (≠ S165), S147 (≠ G166), E200 (= E215), G201 (= G216), I206 (≠ T221), W321 (≠ F342), Y322 (≠ P343), E412 (= E431), H413 (= H432), N449 (= N468)
binding manganese (ii) ion: H368 (= H387), H375 (= H394), E412 (= E431)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:455/455 of 8jduA

query
sites
8jduA

P

P

M

D

C

A

V

V

A

V

L

W

P

P

E

Q

T

N

V

V

D

D

Q

Q

V

V

Q

S

A

R

V

V

L

A

K

S

A

L

C

C

Y

Y

A

N

L

Q

D

G

V

V

P

P

V

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

M

C

P

A

H

V

T

Q

M

G

G

G

V

V

T

C

L

I

S

N

L
|
L

A

T

K

H

F

M

N

D

Q

Q

I

I

L

T

N

E

I

L

D

N

A

T

T

E

A

D

R

F

T

S

A

V

V

V

Q

E

C

P

G

G

V

V

R

T

N

R

L

K

A

A

I

L

S

N

E

T

A

H

V

L

S

R

Q

D

Y

S

G

G

L

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

I

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

V

T

H

N

C

A

L

V

K

R

Y

Y

G

G

L

T

T

M

V

R

H

D

N

N

V

V

L

I

K

N

V

L

K

E

G

V

F

V

T

L

I

P

E

D

G

G

E

R

P

L

I

L

E

H

F

T

G

A

S

G

D

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

T

A

P

A

G

G

L

Y

D

N

L

L

L

T

P

G

V

L

V

F

V

V

G

G

S

S

E
|
E

G
|
G

M

T

L

L

A

G

V

I

T
x
I

T

T

E

S

V

T

T

T

V

L

K

R

L

L

V

H

P

P

K

A

P

P

Q

E

L

A

A

T

R

V

C

A

I

A

M

T

A

C

S

A

F

F

D

P

D

S

V

V

R

Q

K

A

A

A

G

V

D

D

A

S

V

T

A

V

A

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

A

A

G

R

L

I

E

E

M

F

M

L

D

D

G

D

P

V

M

M

T

M

A

D

A

A

V

C

E

N

D

R

F

-

V

-

H

H

A

S

D

K

Y

L

D

N

L

C

S

P

A

V

A

A

A

P

I

T

L

L

L

F

C

L

E

E

S

F

D

H

G

G

T

S

P

Q

E

Q

E

T

V

L

E

A

E

E

E

Q

I

L

G

Q

R

R

M

T

S

E

E

A

V

I

L

T

R

Q

G

N

C

G

G

G

A

S

T

H

A

-

I

-

T

-

V

F

S

S

R

W

D

A

E

A

A

E

Q

K

R

R

M

N

K

E

F

L

W

W

S

A

G

A

R
|
R

K

H

N

N

A

A

F
x
W

P

Y

A

A

S

A

G

L

R

A

I

L

S

S

P

P

-

G

-

S

D

K

Y

A

M

Y

C

S

M

T

D

D

S

V

T

C

I

V

P

P

R

I

K

S

R

R

L

L

A

P

D

E

I

I

L

L

E

V

A

E

I

T

S

K

E

E

M

E

E

I

K

K

K

A

Y

S

Q

K

L

L

R

T

C

G

L

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H
|
H

P

C

L

I

I

L

L

L

F

V

D

D

A

P

N

D

D

D

P

A

D

E

Q

E

M

Q

H

R

R

R

C

V

E

K

Q

A

F

F

G

A

A

E

D

N

I

L

L

G

E

R

T

R

S

A

V

L

A

A

M

L

G

G

T

T

V

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

V

R

N

Q

S

L

M

L

C

Q

V

E

Q

E

F

V

S

G

A

P

E

V

E

G

N

V

E

E

Q

T

M

M

F

R

G

Q

V

L

K

K

R

N

A

T

F

L

D

D

S

P

K

R

G

G

L

L

L

M

N
|
N

P

P

G

G

K

K

V

V

I

L

binding 2-oxopentanoic acid: R318 (= R338), W322 (≠ F342), H369 (= H387), H376 (= H394), H414 (= H432)
binding flavin-adenine dinucleotide: P68 (≠ A85), G70 (= G87), T71 (≠ A88), G72 (= G89), T73 (= T90), G74 (= G91), G78 (= G95), V79 (≠ A96), L90 (= L107), P132 (= P149), G133 (≠ S150), A134 (≠ S151), G140 (= G159), M141 (≠ N160), A143 (= A162), T144 (≠ E163), A146 (≠ S165), S147 (≠ G166), E200 (= E215), G201 (= G216), I206 (≠ T221), W322 (≠ F342), E413 (= E431), N450 (= N468)
binding manganese (ii) ion: H369 (= H387), H376 (= H394), E413 (= E431)

8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:455/455 of 8jdpA

query
sites
8jdpA

P

P

M

D

C

A

V

V

A

V

L

W

P

P

E

Q

T

N

V

V

D

D

Q

Q

V

V

Q

S

A

R

V

V

L

A

K

S

A

L

C

C

Y

Y

A

N

L

Q

D

G

V

V

P

P

V

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

M

C

P

A

H

V

T

Q

M

G

G

G

V

V

T

C

L

I

S

N

L
|
L

A

T

K

H

F

M

N

D

Q

Q

I

I

L

T

N

E

I

L

D

N

A

T

T

E

A

D

R

F

T

S

A

V

V

V

Q

E

C

P

G

G

V

V

R

T

N

R

L

K

A

A

I

L

S

N

E

T

A

H

V

L

S

R

Q

D

Y

S

G

G

L

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

I

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

V

T

H

N

C

A

L

V

K

R

Y

Y

G

G

L

T

T

M

V

R

H

D

N

N

V

V

L

I

K

N

V

L

K

E

G

V

F

V

T

L

I

P

E

D

G

G

E

R

P

L

I

L

E

H

F

T

G

A

S

G

D

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

T

A

P

A

G

G

L

Y

D

N

L

L

L

T

P

G

V

L

V

F

V

V

G

G

S

S

E
|
E

G
|
G

M

T

L

L

A

G

V

I

T
x
I

T

T

E

S

V

T

T

T

V

L

K

R

L

L

V

H

P

P

K

A

P

P

Q

E

L

A

A

T

R

V

C

A

I

A

M

T

A

C

S

A

F

F

D

P

D

S

V

V

R

Q

K

A

A

A

G

V

D

D

A

S

V

T

A

V

A

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

A

A

G

R

L

I

E

E

M

F

M

L

D

D

G

D

P

V

M

M

T

M

A

D

A

A

V

C

E

N

D

R

F

-

V

-

H

H

A

S

D

K

Y

L

D

N

L

C

S

P

A

V

A

A

A

P

I

T

L

L

L

F

C

L

E

E

S

F

D

H

G

G

T

S

P

Q

E

Q

E

T

V

L

E

A

E

E

E

Q

I

L

G

Q

R

R

M

T

S

E

E

A

V

I

L

T

R

Q

G

D

C

N

G

G

A

G

T

S

A

H

I

F

T

S

V

W

S

A

R

A

D

E

E

-

A

-

Q

K

R

R

M

N

K

E

F

L

W

W

S

A

G

A

R
|
R

K

H

N

N

A

A

F

W

P

Y

A

A

S

A

G

L

R

A

I

L

S

S

P

P

D

S

Y

K

-

A

M

Y

C

S

M

T

D

D

S

V

T

C

I

V

P

P

R

I

K

S

R

R

L

L

A

P

D

E

I

I

L

L

E

V

A

E

I

T

S

K

E

E

M

E

E

I

K

K

K

A

Y

S

Q

K

L

L

R

T

C

G

L

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

L

F

H

A

P

C

L

I

I

L

L

L

F

V

D

D

A

P

N

D

D

D

P

A

D

E

Q

E

M

Q

H

R

R

R

C

V

E

K

Q

A

F

F

G

A

A

E

D

N

I

L

L

G

E

R

T

R

S

A

V

L

A

A

M

L

G

G

T

T

V

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

V

R

N

Q

S

L

M

L

C

Q

V

E

Q

E

F

V

S

G

A

P

E

V

E

G

N

V

E

E

Q

T

M

M

F

R

G

Q

V

L

K

K

R

N

A

T

F

L

D

D

S

P

K

R

G

G

L

L

L

M

N
|
N

P

P

G

G

K

K

V

V

I

L

binding flavin-adenine dinucleotide: P68 (≠ A85), G70 (= G87), T71 (≠ A88), G72 (= G89), T73 (= T90), G74 (= G91), G78 (= G95), V79 (≠ A96), L90 (= L107), P132 (= P149), G133 (≠ S150), A134 (≠ S151), G140 (= G159), M141 (≠ N160), A143 (= A162), T144 (≠ E163), A146 (≠ S165), S147 (≠ G166), E200 (= E215), G201 (= G216), I206 (≠ T221), H369 (= H387), E413 (= E431), N450 (= N468)
binding deaminohydroxyvaline: R319 (= R338), H414 (= H432)

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:455/455 of 8jdxA

query
sites
8jdxA

P

P

M

D

C

A

V

V

A

V

L

W

P

P

E

Q

T

N

V

V

D

D

Q

Q

V

V

Q

S

A

R

V

V

L

A

K

S

A

L

C

C

Y

Y

A

N

L

Q

D

G

V

V

P

P

V

I

A
x
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

G
|
G

L

V

S

E

G

G

G
|
G

A
x
V

M

C

P

A

H

V

T

Q

M

G

G

G

V

V

T

C

L

I

S

N

L
|
L

A

T

K

H

F

M

N

D

Q

Q

I

I

L

T

N

E

I

L

D

N

A

T

T

E

A

D

R

F

T

S

A

V

V

V

Q

E

C

P

G

G

V

V

R

T

N

R

L

K

A

A

I

L

S

N

E

T

A

H

V

L

S

R

Q

D

Y

S

G

G

L

L

Y

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

I

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

V

A

A
|
A

E
x
T

N

G

S
x
A

G
x
S

G

G

V

T

H

N

C

A

L

V

K

R

Y

Y

G

G

L

T

T

M

V

R

H

D

N

N

V

V

L

I

K

N

V

L

K

E

G

V

F

V

T

L

I

P

E

D

G

G

E

R

P

L

I

L

E

H

F

T

G

A

S

G

D

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

T

A

P

A

G

G

L

Y

D

N

L

L

L

T

P

G

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x
I

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H

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H

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H

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binding flavin-adenine dinucleotide: P68 (≠ A85), G70 (= G87), T71 (≠ A88), G72 (= G89), T73 (= T90), G74 (= G91), G78 (= G95), V79 (≠ A96), L90 (= L107), P132 (= P149), G133 (≠ S150), A134 (≠ S151), G140 (= G159), M141 (≠ N160), A143 (= A162), T144 (≠ E163), A146 (≠ S165), S147 (≠ G166), E200 (= E215), G201 (= G216), I206 (≠ T221), W322 (≠ F342), E413 (= E431), H414 (= H432), N450 (= N468)
binding 3-methyl-2-oxobutanoic acid: R318 (= R338), H369 (= H387), H376 (= H394), H414 (= H432)
binding manganese (ii) ion: H369 (= H387), H376 (= H394), E413 (= E431)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
29% identity, 83% coverage: 57:473/500 of query aligns to 40:456/456 of 8jdrA

query
sites
8jdrA

P

P

M

D

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A

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