SitesBLAST
Comparing WP_108402835.1 NCBI__GCF_003063475.1:WP_108402835.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
42% identity, 88% coverage: 33:268/268 of query aligns to 27:261/261 of 5jbxB
- active site: A67 (≠ G74), R72 (= R79), L84 (≠ H91), R88 (≠ E95), G112 (= G119), E115 (= E122), T134 (≠ P141), E135 (= E142), I140 (= I147), P142 (= P149), G143 (= G150), A228 (≠ R235), L238 (≠ V245)
- binding coenzyme a: A28 (= A34), A65 (≠ G72), D68 (= D75), L69 (= L76), K70 (= K77), L110 (≠ Y117), G111 (= G118), T134 (≠ P141), E135 (= E142), L138 (= L145), R168 (≠ P175)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
37% identity, 97% coverage: 9:268/268 of query aligns to 3:259/259 of 5zaiC
- active site: A65 (≠ G74), F70 (≠ R79), S82 (≠ Q90), R86 (vs. gap), G110 (= G119), E113 (= E122), P132 (= P141), E133 (= E142), I138 (= I147), P140 (= P149), G141 (= G150), A226 (≠ R235), F236 (≠ V245)
- binding coenzyme a: K24 (≠ V31), L25 (≠ G32), A63 (≠ G72), G64 (= G73), A65 (≠ G74), D66 (= D75), I67 (≠ L76), P132 (= P141), R166 (≠ P175), F248 (= F257), K251 (= K260)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
37% identity, 90% coverage: 26:266/268 of query aligns to 22:258/260 of 2hw5C
- active site: A68 (≠ G74), M73 (≠ R79), S83 (≠ Q90), L87 (≠ F94), G111 (= G119), E114 (= E122), P133 (= P141), E134 (= E142), T139 (≠ I147), P141 (= P149), G142 (= G150), K227 (≠ R235), F237 (≠ V245)
- binding crotonyl coenzyme a: K26 (≠ E30), A27 (≠ V31), L28 (≠ G32), A30 (= A34), K62 (= K68), I70 (≠ L76), F109 (≠ Y117)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
36% identity, 97% coverage: 8:268/268 of query aligns to 1:256/256 of 3h81A
- active site: A64 (≠ G74), M69 (≠ R79), T79 (≠ Q90), F83 (= F94), G107 (= G119), E110 (= E122), P129 (= P141), E130 (= E142), V135 (≠ I147), P137 (= P149), G138 (= G150), L223 (≠ R235), F233 (≠ V245)
- binding calcium ion: F233 (≠ V245), Q238 (≠ R250)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
36% identity, 96% coverage: 8:265/268 of query aligns to 2:254/255 of 3q0jC
- active site: A65 (≠ G74), M70 (≠ R79), T80 (≠ Q90), F84 (= F94), G108 (= G119), E111 (= E122), P130 (= P141), E131 (= E142), V136 (≠ I147), P138 (= P149), G139 (= G150), L224 (≠ R235), F234 (≠ V245)
- binding acetoacetyl-coenzyme a: Q23 (≠ E30), A24 (≠ V31), L25 (≠ G32), A27 (= A34), A63 (≠ G72), G64 (= G73), A65 (≠ G74), D66 (= D75), I67 (≠ L76), K68 (= K77), M70 (≠ R79), F84 (= F94), G107 (= G118), G108 (= G119), E111 (= E122), P130 (= P141), E131 (= E142), P138 (= P149), G139 (= G150), M140 (= M151)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
36% identity, 96% coverage: 8:265/268 of query aligns to 2:254/255 of 3q0gC
- active site: A65 (≠ G74), M70 (≠ R79), T80 (≠ Q90), F84 (= F94), G108 (= G119), E111 (= E122), P130 (= P141), E131 (= E142), V136 (≠ I147), P138 (= P149), G139 (= G150), L224 (≠ R235), F234 (≠ V245)
- binding coenzyme a: L25 (≠ G32), A63 (≠ G72), I67 (≠ L76), K68 (= K77), Y104 (≠ H115), P130 (= P141), E131 (= E142), L134 (= L145)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
37% identity, 96% coverage: 9:266/268 of query aligns to 4:252/254 of 2dubA
- active site: A67 (≠ G74), M72 (≠ R79), S82 (≠ Q90), G105 (= G119), E108 (= E122), P127 (= P141), E128 (= E142), T133 (≠ I147), P135 (= P149), G136 (= G150), K221 (≠ R235), F231 (≠ V245)
- binding octanoyl-coenzyme a: K25 (≠ E30), A26 (≠ V31), L27 (≠ G32), A29 (= A34), A65 (≠ G72), A67 (≠ G74), D68 (= D75), I69 (≠ L76), K70 (= K77), G105 (= G119), E108 (= E122), P127 (= P141), E128 (= E142), G136 (= G150), A137 (≠ M151)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
37% identity, 96% coverage: 9:266/268 of query aligns to 5:258/260 of 1dubA
- active site: A68 (≠ G74), M73 (≠ R79), S83 (≠ Q90), L87 (≠ F94), G111 (= G119), E114 (= E122), P133 (= P141), E134 (= E142), T139 (≠ I147), P141 (= P149), G142 (= G150), K227 (≠ R235), F237 (≠ V245)
- binding acetoacetyl-coenzyme a: K26 (≠ E30), A27 (≠ V31), L28 (≠ G32), A30 (= A34), A66 (≠ G72), A68 (≠ G74), D69 (= D75), I70 (≠ L76), Y107 (≠ H115), G110 (= G118), G111 (= G119), E114 (= E122), P133 (= P141), E134 (= E142), L137 (= L145), G142 (= G150), F233 (≠ Y241), F249 (= F257)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
37% identity, 96% coverage: 9:266/268 of query aligns to 3:256/258 of 1ey3A
- active site: A66 (≠ G74), M71 (≠ R79), S81 (≠ Q90), L85 (≠ F94), G109 (= G119), E112 (= E122), P131 (= P141), E132 (= E142), T137 (≠ I147), P139 (= P149), G140 (= G150), K225 (≠ R235), F235 (≠ V245)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E30), L26 (≠ G32), A28 (= A34), A64 (≠ G72), G65 (= G73), A66 (≠ G74), D67 (= D75), I68 (≠ L76), L85 (≠ F94), W88 (= W99), G109 (= G119), P131 (= P141), L135 (= L145), G140 (= G150)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
36% identity, 96% coverage: 8:265/268 of query aligns to 1:249/250 of 3q0gD
- active site: A64 (≠ G74), M69 (= M82), T75 (≠ Q88), F79 (≠ E95), G103 (= G119), E106 (= E122), P125 (= P141), E126 (= E142), V131 (≠ I147), P133 (= P149), G134 (= G150), L219 (≠ R235), F229 (≠ V245)
- binding Butyryl Coenzyme A: F225 (≠ Y241), F241 (= F257)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
37% identity, 96% coverage: 9:266/268 of query aligns to 35:288/290 of P14604
- E144 (= E122) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E142) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
37% identity, 96% coverage: 9:266/268 of query aligns to 5:256/258 of 1mj3A
- active site: A68 (≠ G74), M73 (≠ R79), S83 (≠ E95), L85 (= L97), G109 (= G119), E112 (= E122), P131 (= P141), E132 (= E142), T137 (≠ I147), P139 (= P149), G140 (= G150), K225 (≠ R235), F235 (≠ V245)
- binding hexanoyl-coenzyme a: K26 (≠ E30), A27 (≠ V31), L28 (≠ G32), A30 (= A34), A66 (≠ G72), G67 (= G73), A68 (≠ G74), D69 (= D75), I70 (≠ L76), G109 (= G119), P131 (= P141), E132 (= E142), L135 (= L145), G140 (= G150)
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
38% identity, 93% coverage: 18:266/268 of query aligns to 18:266/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
35% identity, 93% coverage: 20:268/268 of query aligns to 17:266/266 of O53561
- K135 (≠ R137) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 137:144, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ T144) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
1ef9A The crystal structure of methylmalonyl coa decarboxylase complexed with 2s-carboxypropyl coa (see paper)
31% identity, 88% coverage: 32:268/268 of query aligns to 22:261/261 of 1ef9A
- active site: H66 (≠ G74), L71 (vs. gap), D82 (≠ Q88), R86 (≠ E95), G110 (= G119), E113 (= E122), P133 (≠ E142), V138 (≠ I147), Y140 (≠ P149), N141 (≠ G150), E228 (= E237), Y238 (vs. gap)
- binding 2-carboxypropyl-coenzyme a: A64 (≠ G72), H66 (≠ G74), D67 (= D75), I68 (≠ L76), H69 (≠ K77), W108 (≠ Y117), G110 (= G119), T132 (≠ P141), P133 (≠ E142), K253 (= K260)
P52045 Methylmalonyl-CoA decarboxylase; MMCD; Transcarboxylase; EC 4.1.1.- from Escherichia coli (strain K12) (see paper)
31% identity, 88% coverage: 32:268/268 of query aligns to 22:261/261 of P52045
- G110 (= G119) binding substrate
- T132 (≠ P141) binding substrate
- K253 (= K260) binding substrate
Q13825 Methylglutaconyl-CoA hydratase, mitochondrial; 3-MG-CoA hydratase; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding protein/enoyl-CoA hydratase; Itaconyl-CoA hydratase; EC 4.2.1.18; EC 4.2.1.56 from Homo sapiens (Human) (see 4 papers)
35% identity, 92% coverage: 21:267/268 of query aligns to 89:338/339 of Q13825