SitesBLAST
Comparing WP_108402938.1 NCBI__GCF_003063475.1:WP_108402938.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
42% identity, 94% coverage: 8:648/682 of query aligns to 3:652/692 of 6iunB
- active site: A60 (= A64), F65 (= F69), E73 (≠ G75), H77 (≠ L79), G101 (= G103), E104 (= E106), E124 (= E126), G132 (= G134), K248 (≠ V246), S407 (= S404), H428 (= H425), E440 (= E437), N478 (= N475)
- binding nicotinamide-adenine-dinucleotide: G300 (= G297), T301 (= T298), M302 (= M299), E321 (= E318), T322 (≠ Q319), Y365 (≠ W362), A377 (= A374), V378 (= V375), E380 (= E377), V384 (= V381), V388 (= V385), N405 (= N402), S407 (= S404)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
40% identity, 92% coverage: 17:646/682 of query aligns to 13:667/723 of Q08426
- V40 (= V40) to G: in dbSNP:rs1062551
- I41 (≠ N41) to R: in dbSNP:rs1062552
- T75 (≠ V77) to I: in dbSNP:rs1062553
- K165 (≠ H167) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ S173) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (= A270) to T: in dbSNP:rs2302819
- A325 (≠ L316) to G: in dbSNP:rs1062555
- K346 (= K337) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (≠ N564) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ Q577) to T: in dbSNP:rs1042437
- T606 (≠ R585) to P: in dbSNP:rs1042438
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
40% identity, 92% coverage: 17:646/682 of query aligns to 16:669/723 of 3zw9A
- active site: A64 (= A64), F69 (= F69), G79 (≠ L79), G103 (= G103), E106 (= E106), P125 (= P125), E126 (= E126), P133 (= P133), G134 (= G134), K252 (≠ V246), S413 (= S404), H434 (= H425), E446 (= E437), N484 (= N475)
- binding nicotinamide-adenine-dinucleotide: L305 (≠ I294), G306 (= G295), G308 (= G297), T309 (= T298), M310 (= M299), E329 (= E318), Q334 (≠ A323), A383 (= A374), V384 (= V375), F385 (≠ Y376), E386 (= E377), N411 (= N402), S413 (= S404), H434 (= H425)
- binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (= V25), A62 (= A62), G63 (= G63), A64 (= A64), I66 (= I66), G102 (= G102), G103 (= G103), E106 (= E106), E126 (= E126), P133 (= P133), Y159 (≠ R159)
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
40% identity, 92% coverage: 17:646/682 of query aligns to 18:671/725 of 5omoA
- active site: A66 (= A64), F71 (= F69), G81 (≠ L79), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (≠ V246), S415 (= S404), H436 (= H425), E448 (= E437), N486 (= N475)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P24), V26 (= V25), A28 (= A27), P31 (≠ I30), A64 (= A62), A66 (= A64), D67 (= D65), I68 (= I66), L103 (= L101), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), Y161 (≠ R159), F260 (= F252), K280 (= K272)
- binding 3-keto-decanoyl-coa: S415 (= S404), N486 (= N475), K519 (≠ A508), M520 (= M509), V525 (= V514), Y658 (= Y633)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
40% identity, 92% coverage: 17:646/682 of query aligns to 18:671/725 of 5mgbA
- active site: A66 (= A64), F71 (= F69), G81 (≠ L79), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (≠ V246), S415 (= S404), H436 (= H425), E448 (= E437), N486 (= N475)
- binding acetoacetyl-coenzyme a: P25 (= P24), V26 (= V25), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I66), G105 (= G103), E128 (= E126), Y161 (≠ R159)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ I294), G308 (= G295), G310 (= G297), T311 (= T298), M312 (= M299), E331 (= E318), S332 (≠ Q319), Q336 (≠ A323), V386 (= V375), F387 (≠ Y376), E388 (= E377), N413 (= N402), S415 (= S404), H436 (= H425)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
40% identity, 92% coverage: 17:646/682 of query aligns to 18:671/725 of 3zwcA
- active site: A66 (= A64), F71 (= F69), G81 (≠ L79), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (≠ V246), S415 (= S404), H436 (= H425), E448 (= E437), N486 (= N475)
- binding (s)-3-hydroxydecanoyl-coa: V26 (= V25), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I66), G77 (= G75), L78 (≠ P76), L80 (= L78), V101 (≠ A99), G104 (= G102), G105 (= G103), E108 (= E106), E128 (= E126), F260 (= F252)
- binding nicotinamide-adenine-dinucleotide: G308 (= G295), G310 (= G297), T311 (= T298), M312 (= M299), E331 (= E318), Q336 (≠ A323), A385 (= A374), V386 (= V375), F387 (≠ Y376), E388 (= E377), K393 (= K382), N413 (= N402), S415 (= S404), H436 (= H425)
2x58A The crystal structure of mfe1 liganded with coa (see paper)
40% identity, 92% coverage: 17:646/682 of query aligns to 18:671/725 of 2x58A