SitesBLAST
Comparing WP_108403031.1 NCBI__GCF_003063475.1:WP_108403031.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9HTQ2 Alanine racemase, catabolic; EC 5.1.1.1 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
49% identity, 98% coverage: 3:349/353 of query aligns to 2:351/357 of Q9HTQ2
- K33 (= K34) modified: N6-(pyridoxal phosphate)lysine
- K122 (= K124) modified: N6-carboxylysine
1rcqA The 1.45 a crystal structure of alanine racemase from a pathogenic bacterium, pseudomonas aeruginosa, contains both internal and external aldimine forms (see paper)
49% identity, 98% coverage: 3:349/353 of query aligns to 2:351/357 of 1rcqA
- active site: K33 (= K34), R129 (= R131), H158 (= H160), R208 (= R210), Y253 (= Y252), S300 (= S298), D302 (= D300)
- binding d-lysine: K33 (= K34), R129 (= R131), Y341 (= Y339)
- binding pyridoxal-5'-phosphate: V31 (= V32), K33 (= K34), Y37 (= Y38), H158 (= H160), N192 (= N194), S193 (= S195), R208 (= R210), G210 (= G212), I211 (= I213), Y341 (= Y339)
6a2fA Crystal structure of biosynthetic alanine racemase from pseudomonas aeruginosa (see paper)
42% identity, 97% coverage: 3:345/353 of query aligns to 2:348/358 of 6a2fA
- active site: K34 (= K34), R130 (= R131), H159 (= H160), R209 (= R210), Y254 (= Y252), S301 (= S298), D303 (= D300)
- binding acetate ion: Y254 (= Y252), R279 (= R277), L340 (≠ I337), Y342 (= Y339)
- binding d-lysine: Y254 (= Y252), Y273 (= Y271), S301 (= S298), M302 (= M299), D303 (= D300), M304 (= M301)
- binding malonate ion: K123 (= K124), R130 (= R131), L131 (= L132)
2rjhA Crystal structure of biosynthetic alaine racemase in d-cycloserine- bound form from escherichia coli (see paper)
41% identity, 96% coverage: 8:345/353 of query aligns to 7:349/359 of 2rjhA
- active site: K34 (= K34), R129 (= R131), H159 (= H160), R209 (= R210), Y255 (= Y252), A302 (≠ S298), D304 (= D300)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K34 (= K34), Y38 (= Y38), R129 (= R131), H159 (= H160), A193 (≠ N194), S194 (= S195), R209 (= R210), G211 (= G212), I212 (= I213), Y255 (= Y252), A302 (≠ S298), M303 (= M299), Y343 (= Y339)
P0A6B4 Alanine racemase, biosynthetic; EC 5.1.1.1 from Escherichia coli (strain K12) (see paper)
41% identity, 96% coverage: 8:345/353 of query aligns to 7:349/359 of P0A6B4
- K34 (= K34) modified: N6-(pyridoxal phosphate)lysine
- K122 (= K124) modified: N6-carboxylysine
- D164 (= D165) mutation to A: Slightly reduces affinity for D-Ala and L-Ala.; mutation to K: Reduces catalytic activity. Slightly reduces affinity for D-Ala and L-Ala.
- E165 (≠ V166) mutation to A: Slightly reduces affinity for D-Ala and L-Ala.; mutation to K: Reduces catalytic activity. Slightly reduces affinity for D-Ala and L-Ala.
- P219 (= P220) mutation to A: No effect on catalytic activity. No effect on affinity for D-Ala and L-Ala.
- E221 (vs. gap) mutation E->A,K,P: Slightly increases catalytic activity. Slightly increases affinity for D-Ala and L-Ala.
4xbjA Y274f alanine racemase from e. Coli inhibited by l-ala-p (see paper)
41% identity, 96% coverage: 8:345/353 of query aligns to 5:347/357 of 4xbjA
- active site: K32 (= K34), R127 (= R131), H157 (= H160), R207 (= R210), Y253 (= Y252), A300 (≠ S298), D302 (= D300)
- binding {1-[(3-hydroxy-methyl-5-phosphonooxy-methyl-pyridin-4-ylmethyl)-amino]-ethyl}-phosphonic acid: V30 (= V32), K32 (= K34), Y36 (= Y38), R127 (= R131), H157 (= H160), A191 (≠ N194), S192 (= S195), R207 (= R210), G209 (= G212), I210 (= I213), Y253 (= Y252), A300 (≠ S298), M301 (= M299), Y341 (= Y339)
3b8tA Crystal structure of escherichia coli alaine racemase mutant p219a (see paper)
41% identity, 96% coverage: 8:345/353 of query aligns to 7:349/359 of 3b8tA
- active site: K34 (= K34), R129 (= R131), H159 (= H160), R209 (= R210), Y255 (= Y252), A302 (≠ S298), D304 (= D300)
- binding pyridoxal-5'-phosphate: V32 (= V32), K34 (= K34), Y38 (= Y38), L78 (= L79), H159 (= H160), A193 (≠ N194), S194 (= S195), R209 (= R210), G211 (= G212), I212 (= I213), Y343 (= Y339)
P0A2W8 Alanine racemase; EC 5.1.1.1 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
35% identity, 97% coverage: 3:343/353 of query aligns to 7:356/367 of P0A2W8
- K129 (= K124) modified: N6-carboxylysine
6g58A Structure of the alanine racemase from staphylococcus aureus in complex with a pyridoxal 5' phosphate-derivative (see paper)
36% identity, 96% coverage: 9:347/353 of query aligns to 13:362/382 of 6g58A
- active site: K39 (= K34), R138 (= R131), H168 (= H160), R219 (= R210), Y265 (= Y252), C311 (≠ S298), D313 (= D300)
- binding (6-but-3-ynyl-4-methyl-5-oxidanyl-pyridin-3-yl)methyl dihydrogen phosphate: K39 (= K34), Y43 (= Y38), L85 (= L79), K131 (= K124), R138 (= R131), H168 (= H160), S204 (= S195), R219 (= R210), G221 (= G212), I222 (= I213), Y354 (= Y339)
6g59A Structure of the alanine racemase from staphylococcus aureus in complex with an pyridoxal-6- phosphate derivative (see paper)
36% identity, 96% coverage: 9:347/353 of query aligns to 18:367/387 of 6g59A
- active site: K44 (= K34), R143 (= R131), H173 (= H160), R224 (= R210), Y270 (= Y252), C316 (≠ S298), D318 (= D300)
- binding (6-ethynyl-4-methanoyl-5-oxidanyl-pyridin-3-yl)methyl dihydrogen phosphate: K44 (= K34), Y48 (= Y38), L90 (= L79), H173 (= H160), S209 (= S195), R224 (= R210), G226 (= G212), I227 (= I213), Y359 (= Y339)
3e5pB Crystal structure of alanine racemase from e.Faecalis (see paper)
34% identity, 98% coverage: 3:347/353 of query aligns to 7:364/371 of 3e5pB
- active site: K40 (= K34), R139 (= R131), H169 (= H160), R222 (= R210), Y267 (= Y252), C313 (≠ S298), D315 (= D300)
- binding pyridoxal-5'-phosphate: K40 (= K34), Y44 (= Y38), R139 (= R131), H169 (= H160), S207 (= S195), G224 (= G212), V225 (≠ I213), Y356 (= Y339)
3e6eA Crystal structure of alanine racemase from e.Faecalis complex with cycloserine (see paper)
34% identity, 98% coverage: 3:347/353 of query aligns to 6:363/370 of 3e6eA
- active site: K39 (= K34), R138 (= R131), H168 (= H160), R221 (= R210), Y266 (= Y252), C312 (≠ S298), D314 (= D300)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K39 (= K34), Y43 (= Y38), L85 (= L79), R138 (= R131), H168 (= H160), S206 (= S195), R221 (= R210), G223 (= G212), V224 (≠ I213), Y266 (= Y252), Y285 (= Y271), M313 (= M299), Y355 (= Y339)
7xllA Alanine racemase from lactobacillus sakei uonuma-1. (see paper)
33% identity, 97% coverage: 3:345/353 of query aligns to 7:366/378 of 7xllA