SitesBLAST
Comparing WP_109739131.1 NCBI__GCF_002899945.2:WP_109739131.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
29% identity, 98% coverage: 2:305/309 of query aligns to 3:299/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I145), G147 (= G146), L148 (≠ Y147), G149 (= G148), R150 (≠ N149), I151 (≠ M150), G152 (= G151), D170 (= D169), H201 (= H197), T202 (≠ I198), P203 (= P199)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
29% identity, 98% coverage: 2:305/309 of query aligns to 3:299/302 of 6rihA
7dkmA Phgdh covalently linked to oridonin (see paper)
29% identity, 98% coverage: 2:305/309 of query aligns to 4:300/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ A72), A102 (≠ V100), G148 (= G146), R151 (≠ N149), I152 (≠ M150), Y170 (≠ H168), D171 (= D169), P172 (vs. gap), I173 (vs. gap), H202 (= H197), T203 (≠ I198), P204 (= P199), T209 (= T204), C230 (≠ T225), A231 (= A226), R232 (= R227), H279 (= H284), G281 (≠ A286)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ L12), K17 (≠ E15), I18 (≠ Q16), E293 (≠ Q298)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
29% identity, 98% coverage: 2:305/309 of query aligns to 3:299/301 of 6rj5A
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
29% identity, 98% coverage: 2:305/309 of query aligns to 4:300/305 of 6plfA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
29% identity, 98% coverage: 2:305/309 of query aligns to 2:298/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N96), A100 (≠ V100), R149 (≠ N149), I150 (≠ M150), Y168 (≠ H168), D169 (= D169), P170 (vs. gap), I171 (vs. gap), H200 (= H197), T201 (≠ I198), P202 (= P199), T207 (= T204), C228 (≠ T225), A229 (= A226), R230 (= R227), H277 (= H284), G279 (≠ A286)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
29% identity, 98% coverage: 2:305/309 of query aligns to 3:299/303 of 6plgA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
29% identity, 97% coverage: 2:301/309 of query aligns to 2:294/297 of 6rj3A
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
30% identity, 98% coverage: 2:305/309 of query aligns to 8:304/533 of O43175
- T78 (≠ A72) binding NAD(+)
- R135 (= R129) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ NM 149:150) binding NAD(+)
- D175 (= D169) binding NAD(+)
- T207 (≠ I198) binding NAD(+)
- CAR 234:236 (≠ TAR 225:227) binding NAD(+)
- D260 (= D251) binding NAD(+)
- V261 (= V252) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIAG 284:287) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
2eklA Structure of st1218 protein from sulfolobus tokodaii
34% identity, 87% coverage: 31:299/309 of query aligns to 35:297/312 of 2eklA
- active site: S100 (≠ N96), R232 (= R227), D256 (= D251), E261 (≠ K257), H282 (= H284)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ A72), S100 (≠ N96), G148 (= G146), G150 (= G148), R151 (≠ N149), I152 (≠ M150), Y170 (≠ H168), D171 (= D169), I172 (= I170), L173 (= L171), H202 (= H197), V203 (≠ I198), T204 (≠ P199), I212 (≠ L207), T230 (= T225), S231 (≠ A226), D256 (= D251), G284 (≠ A286)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
29% identity, 98% coverage: 3:305/309 of query aligns to 1:296/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G148), I148 (≠ M150), Y166 (≠ H168), D167 (= D169), P168 (vs. gap), I169 (vs. gap), I170 (= I170), H198 (= H197), T199 (≠ I198), L208 (= L207), R228 (= R227)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
33% identity, 88% coverage: 33:305/309 of query aligns to 33:299/304 of 1wwkA
- active site: S96 (≠ N96), R230 (= R227), D254 (= D251), E259 (= E256), H278 (= H284)
- binding nicotinamide-adenine-dinucleotide: V100 (= V100), G146 (= G146), F147 (≠ Y147), G148 (= G148), R149 (≠ N149), I150 (≠ M150), Y168 (≠ H168), D169 (= D169), P170 (≠ I170), V201 (≠ I198), P202 (= P199), T207 (= T204), T228 (= T225), S229 (≠ A226), D254 (= D251), H278 (= H284), G280 (≠ A286)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
30% identity, 87% coverage: 38:305/309 of query aligns to 34:290/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ N149), Y160 (≠ H168), D161 (= D169), P162 (vs. gap), I164 (= I170), L179 (= L184), T193 (≠ I198), P194 (= P199), S198 (≠ E203), L202 (= L207)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 95% coverage: 1:293/309 of query aligns to 56:353/466 of P87228
- S87 (= S31) modified: Phosphoserine
- S258 (≠ T201) modified: Phosphoserine
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
30% identity, 98% coverage: 3:304/309 of query aligns to 4:297/526 of 3dc2A