SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_109942363.1 NCBI__GCF_003173335.1:WP_109942363.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
45% identity, 94% coverage: 8:317/329 of query aligns to 9:285/285 of 4yerA

query
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4yerA

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binding adenosine-5'-diphosphate: F14 (= F13), F17 (≠ L16), N39 (= N38), G40 (= G39), G42 (= G41), K43 (= K42), T44 (≠ S43), T45 (= T44), T135 (= T134), F136 (≠ Y135), S137 (= S136)

Q9BZC7 ATP-binding cassette sub-family A member 2; ATP-binding cassette transporter 2; ATP-binding cassette 2; EC 7.6.2.- from Homo sapiens (Human) (see paper)
41% identity, 63% coverage: 18:225/329 of query aligns to 1006:1209/2435 of Q9BZC7

query
sites
Q9BZC7

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Sites not aligning to the query:

271 modified: N5-methylglutamine; Q→R: Abolishes methylation by N6AMT1.

Q99758 Phospholipid-transporting ATPase ABCA3; ABC-C transporter; ATP-binding cassette sub-family A member 3; ATP-binding cassette transporter 3; ATP-binding cassette 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Homo sapiens (Human) (see 15 papers)
40% identity, 65% coverage: 16:228/329 of query aligns to 1396:1605/1704 of Q99758

query
sites
Q99758

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L1553 (≠ I173) to P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs121909183
L1580 (= L203) to P: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; affects the intracellular vesicles development; decreases phosphatidylcholine transport; mutation to A: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; mutation to F: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; mutation to V: Decreases ATP hydrolysis activity of 56% compared to the wild-type.
Q1591 (= Q214) to P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs28936691

Sites not aligning to the query:

43 R → L: in SMDP3; uncertain significance
53 N→Q: Does not affect N-glycosylation. Does not affect protein expression. Does not affect lamellar body membrane location.
101 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; dbSNP:rs121909182
124 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-140. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
140 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → H: in dbSNP:rs45447801; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-124. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
173:174 LK→AA: Loss of proteolytic processing.
174:175 Cleavage; by CTSL
215 Q → K: in SMDP3; loss of lamellar bodies membrane location; loss of proteolytic cleavage; increases cellular free cholesterol and phosphatidylcholine transport; loss of vesicles formation; increases free cholesterol induced cell death; loss of protein oligomerization; dbSNP:rs879159551
280 R → C: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs201299260
288 R → K: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs117603931
290 L → M: in a breast cancer sample; somatic mutation
292 E → V: in SMDP3; uncertain significance; does not affect lamellar bodies membrane location; does not affect proteolytic cleavage; affects lamellar bodies formation; does not affect cholesterol and phosphatidylcholine transport; decreases vesicles formation; does not affect free cholesterol induced cell death; dbSNP:rs149989682
568 N → D: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; does not affect protein expression; does not affect multivesicular bodies and lamellar bodies location; affects multivesicular bodies and lamellar bodies development; loss of phosphatidylcholine transport; does not affect cholesterol transport; dbSNP:rs121909184
579 L → P: in SMDP3; uncertain significance
605 R → Q: in SMDP3; uncertain significance; dbSNP:rs760006956
693 S→L: Does not affect protein oligomerization.
766 P → S: in dbSNP:rs45592239
801 E → D: in a breast cancer sample; somatic mutation
945 N→Q: Does not affect lamellar body membrane location. Does not affect protein expression. Does not affect proteolytic processing.
982 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs1402761450
1069 H → Q: in a breast cancer sample; somatic mutation
1076 N → K: in SMDP3; uncertain significance; dbSNP:rs2093663770
1221 G → S: in SMDP3; does not affect intracellular vesicle membrane location; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; G→A: Decreases ATP hydrolysis activity of 15% compared to the wild-type.; G→T: Decreases ATP hydrolysis activity of 36% compared to the wild-type.; G→V: Decreases ATP hydrolysis activity of 18% compared to the wild-type.
1302 G → E: in SMDP3; uncertain significance; dbSNP:rs2093657978
1388 K → N: in SMDP3; decreases phosphatidylcholine transport; increases protein abundance; does not affect folding in the endoplasmic reticulum; decreases proteolytic processing; affects lamellar bodies development; reduces free cholesterol transport

F1MWM0 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM protein; RmP; Retinal-specific ATP-binding cassette transporter; EC 7.6.2.1 from Bos taurus (Bovine) (see 2 papers)
35% identity, 89% coverage: 18:309/329 of query aligns to 1952:2250/2281 of F1MWM0

query
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F1MWM0

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Sites not aligning to the query:

415 modified: carbohydrate, N-linked (Hex...) asparagine
504 modified: carbohydrate, N-linked (Hex...) asparagine
901 modified: Phosphothreonine; T→A: Decreases expression level. Affects subcellular location.
1185 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1309 Cleavage; by trypsin
1313 modified: Phosphothreonine; T→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1317 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Affects both the basal and stimulated ATPase activity.
1319 modified: Phosphoserine
1455 modified: carbohydrate, N-linked (Hex...) asparagine
1527 modified: carbohydrate, N-linked (Hex...) asparagine
1660 modified: carbohydrate, N-linked (Hex...) asparagine

Q8R420 Phospholipid-transporting ATPase ABCA3; ATP-binding cassette sub-family A member 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Mus musculus (Mouse) (see paper)
39% identity, 66% coverage: 12:228/329 of query aligns to 1392:1605/1704 of Q8R420

query
sites
Q8R420

Q

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Sites not aligning to the query:

292 E→V: Knockin new born mice are healthy and survive into adulthood without overt signs of respiratory distress. Knockin mice show a severe lung phenotype that begins with alveolar inflammatory cell infiltration at the early stage of the mouse life followed by aberrant lung remodeling with characteristics of diffuse parenchymal lung disease (DPLD)- and emphysema-like alveolar disruption in older mice.

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 43 papers)
34% identity, 93% coverage: 4:309/329 of query aligns to 1938:2252/2273 of P78363

query
sites
P78363

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L1940 (≠ T6) to P: in STGD1 and FFM; dbSNP:rs61753033
E1942 (≠ N8) to Q: in STGD1; uncertain significance; dbSNP:rs760353830
P1948 (vs. gap) to L: in dbSNP:rs56142141; natural variant: P -> S
G1961 (≠ A25) to E: in STGD1, FFM and CORD3; risk factor for ARMD2; also found in patients with cone dystrophy and with macular dystrophy; inhibition of ATP hydrolysis by retinal; dbSNP:rs1800553; to R: in STGD1; uncertain significance; dbSNP:rs142253670
L1970 (= L34) to F: in ARMD2, FFM and STGD1; also found in a patient with cone dystrophy; dbSNP:rs28938473
L1971 (= L35) to R: in FFM; highly reduced ATP-binding capacity; abolishes basal and retinal-stimulated ATP hydrolysis; dbSNP:rs61753034
1972:1980 (vs. 36:44, 78% identical) binding ATP
G1975 (= G39) mutation to D: Inhibition of retinal-stimulated ATP hydrolysis.
G1977 (= G41) to S: in STGD1 and ARMD2; highly reduced ATP-binding capacity; inhibition of ATP hydrolysis by retinal; dbSNP:rs61750639
K1978 (= K42) mutation to M: Inhibits ATPase activity; when associated with M-969. Decreases translocase activity; when associated with M-969. Does not affect protein subcellular localization in endoplasmic reticulum; when associated with M-969. Loss of ATP-dependent all-trans-retinal transport; when associated with M-1978. Loss in N-retinylidene-PE transfer activity. Inhibits ATPase activity with increasing retinal concentration. Does not affect ATP-independent N-retinylidene-PE binding. Does not affect ATP-dependent of N-retinylidene-PE release. Significantly reduces PE flippase activity. Inhibition of retinal-stimulated ATP hydrolysis.
C2017 (≠ V81) to Y: in STGD1; uncertain significance
I2023 (= I87) to T: in STGD1; uncertain significance; dbSNP:rs150633517
L2027 (= L91) to F: in STGD1 and FFM; also found in a patient with chorioretinal atrophy; highly reduced ATP-binding capacity; dbSNP:rs61751408
R2030 (= R94) to Q: in STGD1 and FFM; dbSNP:rs61750641
H2032 (≠ N96) to R: in STGD1; uncertain significance; dbSNP:rs1242866408
L2033 (= L97) to R: in STGD1; uncertain significance; dbSNP:rs1553186896
R2038 (≠ M102) to W: in STGD1; highly reduced ATP-binding capacity; dbSNP:rs61750643
R2040 (≠ Y104) to Q: in STGD1; uncertain significance; dbSNP:rs148460146
V2042 (= V106) to G: in STGD1; uncertain significance
P2043 (= P107) to S: in CORD3; uncertain significance; dbSNP:rs763230559
V2050 (≠ R114) to L: in STGD1 and CORD3; may act as a modifier of macular dystrophy in patients who also have a Trp-172 mutation in PRPH2; dbSNP:rs41292677
G2059 (≠ E123) natural variant: G -> A
A2064 (= A128) to T: in STGD1; uncertain significance; dbSNP:rs61753040
GG 2073:2074 (= GG 137:138) binding ATP
G2074 (= G138) to V: in STGD1; uncertain significance; dbSNP:rs367839100
R2077 (= R141) to W: in STGD1; highly reduced ATP-binding capacity; decreases solubility at 50 %; loss of intracellular vesicle localization; drastically reduced basal activity with little or no substrate stimulation; dbSNP:rs61750645
K2078 (≠ R142) to E: in STGD1; uncertain significance
E2096 (= E160) to K: in STGD1; inhibition of ATP hydrolysis by retinal; dbSNP:rs61750646; mutation to Q: Does not affect protein folding; when associated with Q-1087. Loss of ATPase activity; when associated with Q-1087.
P2097 (= P161) to S: in STGD1; uncertain significance; dbSNP:rs1166357291
R2106 (= R170) to C: in STGD1 and FFM; reduced ATP-binding capacity; dbSNP:rs61750648
R2107 (≠ E171) to C: in STGD1; uncertain significance; dbSNP:rs2297669; to H: in STGD1 and CORD3; may predispose to develop retinal toxicity after treatment with chloroquine and hydroxychloroquine; dbSNP:rs62642564; mutation to P: Highly decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
E2131 (≠ D198) to K: in STGD1; uncertain significance; dbSNP:rs61750652
L2140 (≠ V207) to Q: in STGD1; uncertain significance; dbSNP:rs774475956
C2150 (≠ V217) to Y: in STGD1 and CORD3; dbSNP:rs61751384
D2177 (≠ E241) to N: in CORD3, ARMD2 and STGD1; uncertain significance; increased retinal-stimulated ATP hydrolysis; dbSNP:rs1800555
P2180 (≠ A244) mutation to L: Does not affect protein abundance. Does not affect ATPase activity. Moderately decreased phospholipid translocase activity.
F2188 (vs. gap) to S: in STGD1; uncertain significance; dbSNP:rs61750658
A2216 (≠ Q275) to V: in dbSNP:rs886044763
L2221 (vs. gap) to P: in STGD1; uncertain significance; dbSNP:rs1367504380
T2237 (≠ H294) to P: in STGD1; uncertain significance; dbSNP:rs1659051890
VFVNFA 2244:2249 (≠ VFLHYT 301:306) Essential for ATP binding and ATPase activity

Sites not aligning to the query:

14 N → K: in STGD1; uncertain significance
18 R → P: in STGD1; uncertain significance; dbSNP:rs868543294
21:2273 natural variant: Missing (in STGD1; uncertain significance)
24 R → H: in STGD1; uncertain significance; dbSNP:rs62645958
53:2273 natural variant: Missing (in CORD3; uncertain significance; dbSNP:rs764744217)
54 modified: Disulfide link with 81
55 H → R: in CORD3; uncertain significance
63 S → P: in CORD3; uncertain significance
65 G → E: in STGD1 and CORD3; dbSNP:rs62654395
72 G → R: in STGD1; does not affect intracellular vesicle localization; does not affect solubility; significantly reduces N-Ret-PE binding; drastically reduces basal ATPase activity with little or no all trans retinal stimulation; dbSNP:rs61751412; G → V: in STGD1; uncertain significance; dbSNP:rs2101162548
75 modified: Disulfide link with 324
81 modified: Disulfide link with 54
89:2273 natural variant: Missing (in STGD1; uncertain significance)
96 N → H: in STGD1; uncertain significance; dbSNP:rs61748529; N → K: in STGD1; uncertain significance; dbSNP:rs886039297
97 Y → C: in STGD1; uncertain significance; dbSNP:rs755691060
98 modified: carbohydrate, N-linked (GlcNAc...) asparagine
100 S → P: in STGD1; highly decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs61748530
107:2273 natural variant: Missing (in CORD3; uncertain significance; dbSNP:rs765429911)
108 D → V: in STGD1; uncertain significance; dbSNP:rs1662508600
143 P → L: in STGD1; uncertain significance; dbSNP:rs62646860
172 G → S: in STGD1; uncertain significance; dbSNP:rs61748532
184 S → F: in STGD1; uncertain significance; S → R: in STGD1; uncertain significance
185:2273 natural variant: Missing (in STGD1; uncertain significance)
206 S → R: in STGD1; reduced basal and retinal-stimulated ATP-hydrolysis; dbSNP:rs61748536
212 R → C: in STGD1 and CORD3; common mutation in southern Europe; reduced ATP-binding capacity; dbSNP:rs61750200; R → H: in dbSNP:rs6657239
218:2273 natural variant: Missing (in CORD3; uncertain significance)
219:2273 natural variant: Missing (found in a patient with chorioretinal atrophy; uncertain significance)
223 K → Q: in STGD1; uncertain significance; dbSNP:rs147619585
224 T → M: in a breast cancer sample; somatic mutation; dbSNP:rs373540612
240 I → R: in STGD1; uncertain significance; dbSNP:rs1553195472
241 E → D: in STGD1; uncertain significance
245:2273 natural variant: Missing (in STGD1; uncertain significance)
246 A → T: in STGD1; uncertain significance; dbSNP:rs1662207986
290 R → W: in STGD1; uncertain significance; dbSNP:rs781716640
291 P → L: in STGD1; uncertain significance; dbSNP:rs190540405
320 S → C: in CORD3; uncertain significance
324 modified: Disulfide link with 75
326:2273 natural variant: Missing (in STGD1; uncertain significance; dbSNP:rs747540967)
339:2273 natural variant: Missing (in CORD3; uncertain significance)
345 Y → S: in STGD1; uncertain significance; dbSNP:rs1417184535
370 modified: Disulfide link with 519
407 A → V: in STGD1 and CORD3; dbSNP:rs61751264
410 I → T: in STGD1; uncertain significance
415 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → K: in STGD1; uncertain significance; dbSNP:rs1661605073
418 F → S: in STGD1; uncertain significance; dbSNP:rs794726979
423 H → R: in dbSNP:rs3112831
424 V → A: in STGD1 and RP19; uncertain significance; dbSNP:rs1661603358
431:2273 natural variant: Missing (in STGD1; uncertain significance)
440 Y → C: in CORD3; uncertain significance; dbSNP:rs770439859
444 modified: carbohydrate, N-linked (GlcNAc...) asparagine
455 L → M: in RP19; uncertain significance; dbSNP:rs764170051
471 E → K: in ARMD2 and STGD1; uncertain significance; ATP-binding capacity and retinal stimulation as in wild-type; dbSNP:rs1800548
498 D → E: in STGD1; uncertain significance; dbSNP:rs1661575300
504 modified: carbohydrate, N-linked (GlcNAc...) asparagine
508 R → C: in STGD1; uncertain significance; dbSNP:rs138157885
511 R → C: in STGD1; uncertain significance; dbSNP:rs752786160
519 modified: Disulfide link with 370; C → R: in STGD1; uncertain significance; dbSNP:rs1224959251
533:2273 natural variant: Missing (in STGD1; uncertain significance)
541 L → P: in STGD1, FFM and CORD3; reduced ATP-binding capacity; abolishes retinal-stimulated ATP hydrolysis; does not affect solubility; does not affect intracellular vesicle localization; significantly reduces substrate binding; drastically reduces basal ATPase activity with little or no substrate stimulation; dbSNP:rs61751392
548 W → R: in STGD1; uncertain significance
552 V → I: in RP19; uncertain significance; dbSNP:rs145525174
572:2273 natural variant: Missing (in STGD1; uncertain significance)
576 D → H: found in a patient with pattern dystrophy; uncertain significance; dbSNP:rs374224955
593 P → L: in STGD1; uncertain significance
603 Y → C: in STGD1; uncertain significance
605:2273 natural variant: Missing (in CORD3; uncertain significance)
608 F → I: in STGD1; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs61752398
616 E → K: in STGD1; uncertain significance; dbSNP:rs1557787473
636 Q → K: in CORD3; uncertain significance
639:2273 natural variant: Missing (in STGD1; uncertain significance)
640 P → L: in STGD1; uncertain significance; dbSNP:rs760790294
641 modified: Disulfide link with 1490, Interchain; C → S: in STGD1; uncertain significance; dbSNP:rs61749416
643 V → G: in CORD3; uncertain significance; dbSNP:rs61754024
653 R → C: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; very low substrate binding; dbSNP:rs61749420; R → H: in STGD1; uncertain significance; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; very low substrate binding; dbSNP:rs141823837
661 L → R: in CORD3; uncertain significance; severely decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; very low substrate binding
681:2273 natural variant: Missing (found in a patient with macular dystrophy; uncertain significance)
686 L → S: in STGD1; severely decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; very low substrate binding; dbSNP:rs61752402
690 G → V: in STGD1; uncertain significance; severely decreases solubility; loss of cytoplasmic vesicle localization; very low substrate binding; dbSNP:rs942734318
700:2273 natural variant: Missing (in STGD1; uncertain significance)
716 T → M: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; decreases N-Ret-PE binding in the range of 40-70%; dbSNP:rs61749426
754 F → S: in STGD1; uncertain significance
764 C → Y: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; decreases N-Ret-PE binding in the range of 40-70%; dbSNP:rs61749428
765 S → N: in STGD1; severely decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; very low substrate binding; dbSNP:rs61749429; S → R: in STGD1; severely decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; very low substrate binding; dbSNP:rs61752404
767 V → D: in STGD1; also found in a patient with macular dystrophy; severely decreases solubility; loss of cytoplasmic vesicle localization; decreases basal ATPase activity below 50%; severely decreases N-Ret-PE-stimulated ATPase activity; very low substrate binding; dbSNP:rs61751395
779:2273 natural variant: Missing (in STGD1; uncertain significance)
782:2273 natural variant: Missing (in STGD1; uncertain significance)
797 L → P: in STGD1; severely decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; very low substrate binding; dbSNP:rs61749432
808:2273 natural variant: Missing (in STGD1; uncertain significance)
816 G → V: in STGD1; uncertain significance
818 G → E: in STGD1; reduced ATP-binding capacity; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; very low substrate binding; dbSNP:rs61750202
821 W → R: in STGD1; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; very low substrate binding; dbSNP:rs61749433
824 I → T: in STGD1; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; very low substrate binding; dbSNP:rs1660942697
840 M → R: in STGD1; uncertain significance; severely decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; very low substrate binding
846 D → H: severely decreases solubility; loss of cytoplasmic vesicle localization; decreases basal ATPase activity below 50%; severely decreases N-Ret-PE-stimulated ATPase activity; very low substrate binding; dbSNP:rs61754027
849 V → A: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; decreases N-Ret-PE binding in the range of 40-70%; dbSNP:rs61749435
851 G → D: in STGD1; highly reduced ATP-binding capacity; severely decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; very low substrate binding; dbSNP:rs61749436
854 A → T: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; decreases N-Ret-PE binding in the range of 40-70%; dbSNP:rs61749437
863 G → A: in STGD1, FFM and CORD3; also found in a patient with bull's eye maculopathy; mild alteration probably leading to disease phenotype only in combination with a more severe allele; frequent mutation in northern Europe in linkage disequilibrium with the polymorphic variant Q-943; reduced ATP-binding capacity and retinal-stimulated ATP hydrolysis; significantly attenuates 11-cis-retinal binding; decreases about 80% the N-retinylidene-phosphatidylethanolamine transport activity; stimulates modestely the retinal-stimulated ATPase activity; does not affect ATP-independent N-retinylidene-phosphatidylethanolamine binding. Does not affect ATP-dependent release of N-retinylidene-phosphatidylethanolamine; significantly reduces phosphatidylethanolamine flippase activity; dbSNP:rs76157638; natural variant: Missing (in STGD1 and CORD3; reduced ATP-binding capacity and retinal-stimulated ATP hydrolysis)
876:2273 natural variant: Missing (in STGD1; uncertain significance)
901 T → A: in dbSNP:rs61754030
914 natural variant: H -> R
940 P→R: Decreases 11-cis-Retinal binding affinity by 50%.
943 R → Q: risk factor for STGD1; risk factor for ARMD2; decreases 11-cis-Retinal binding affinity by 100-fold; dbSNP:rs1801581; R → W: in STGD1 and FFM; dbSNP:rs61749446
954 Y → D: in STGD1; uncertain significance; dbSNP:rs61749447
959 T → I: in STGD1; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs61752409
963:970 binding ATP
965 N → S: in STGD1; reduced retinal-stimulated ATP hydrolysis; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; decreases about 60% the N-retinylidene-phosphatidylethanolamine transfer activity; stimulates modestly the retinal-stimulated ATPase activity; does not affect ATP-independent N-retinylidene-phosphatidylethanolamine binding; does not affect ATP-dependent release of N-retinylidene-phosphatidylethanolamine; significantly reduces phosphatidylethanolamine flippase activity; dbSNP:rs201471607; N → Y: in STGD1; uncertain significance; dbSNP:rs61749449
966 G→D: Abolishes basal and retinal-stimulated ATP hydrolysis.
969 K→M: Abolishes basal and retinal-stimulated ATP hydrolysis.; K→M: Inhibits ATPase activity; when associated with M-1978. Decreases translocase activity; when associated with M-1978. Does not affect protein subcellular localization in endoplasmic reticulum; when associated with M-1978. Loss of ATP-dependent all-trans-retinal transport; when associated with M-1978. Loss in N-retinylidene-PE transfer activity. Inhibits ATPase activity with increasing retinal concentration. Does not affect N-retinylidene-PE binding. Impairs ATP-dependent release of N-retinylidene-PE. Significantly reduces PE flippase activity.
970 T → P: in STGD1; uncertain significance; dbSNP:rs1570377849
971 T → N: in STGD1; highly reduced ATP-binding capacity; abolishes retinal-stimulated ATP hydrolysis; dbSNP:rs61749450
972 T → N: in STGD1; uncertain significance; dbSNP:rs61749451
973 L → S: in STGD1; uncertain significance
977 T → P: in STGD1; uncertain significance; dbSNP:rs1660625648
978 G → D: in STGD1; uncertain significance; dbSNP:rs61749453
991 G → R: in FFM and STGD1; dbSNP:rs61749455
1022 E → G: in STGD1; uncertain significance; E → K: in STGD1; uncertain significance; dbSNP:rs61749459
1029:2273 natural variant: Missing (in STGD1; uncertain significance)
1038 A → V: in STGD1, FFM and CORD3; frequent mutation; reduced ATP-binding and retinal-stimulated ATP hydrolysis; decreases solubility at 70%; does not affect intracellular vesicle localization; significantly reduces substrate binding in the absence of ATP; reduces basal ATPase activity; dbSNP:rs61751374
1050 G → D: in STGD1; uncertain significance; dbSNP:rs61750062
1054 binding ATP
1071 S → L: in STGD1; reduced ATP-binding capacity; dbSNP:rs61750065
1074 I → L: in STGD1; uncertain significance
1078 G → E: in STGD1; uncertain significance; dbSNP:rs1660532440
1087 E→Q: Does not affect protein folding; when associated with Q-2096. Loss of ATPase activity; when associated with Q-2096.
1091 G → E: in FFM and STGD1; decreases solubilized at 70%; does not affect intracellular vesicle localization; does not affect substrate binding; drastically reduces basal ATPase activity with little or no substrate stimulation; dbSNP:rs61752417
1094 P → T: in STGD1; uncertain significance
1097 R → S: in STGD1; uncertain significance; dbSNP:rs61750118
1098 R → C: in STGD1; uncertain significance; dbSNP:rs756840095
1099 S → P: in STGD1; uncertain significance; dbSNP:rs61750119
1099:2273 natural variant: Missing (in STGD1; uncertain significance)
1102 D → Y: in dbSNP:rs138641544
1108 R → C: in STGD1 and FFM; reduced ATP-binding capacity; dbSNP:rs61750120
1122 E → K: in STGD1 and CORD3; dbSNP:rs61751399
1129 R → C: in STGD1; may predispose to develop retinal toxicity after treatment with chloroquine and hydroxychloroquine; dbSNP:rs779426136; R → L: in ARMD2 and STGD1; also found in patients with fundus flavimaculatus; reduced ATP-binding capacity; dbSNP:rs1801269
1130 I → T: in STGD1; uncertain significance; dbSNP:rs1064793010
1140 C → W: in STGD1; uncertain significance; dbSNP:rs2101048569
1145 L → H: in CORD3; uncertain significance
1148 natural variant: K -> T
1159 L → S: in STGD1; uncertain significance; dbSNP:rs1340749727
1161 R → H: in STGD1; uncertain significance; dbSNP:rs768278935
1177:2273 natural variant: Missing (in STGD1; uncertain significance)
1183 G → C: in CORD3; uncertain significance; dbSNP:rs75267647
1201 L → R: in STGD1; may predispose to develop retinal toxicity after treatment with chloroquine and hydroxychloroquine; dbSNP:rs61750126
1203 G → E: in CORD3; uncertain significance; dbSNP:rs146786552; G → R: in STGD1; uncertain significance
1204 D → N: in STGD1; uncertain significance; dbSNP:rs61750127
1209 M → T: in dbSNP:rs76258939
1253 T → M: in FFM; uncertain significance; dbSNP:rs61752424
1300 R → Q: in STGD1; uncertain significance; dbSNP:rs61750129
1300:2273 natural variant: Missing (in STGD1; uncertain significance; dbSNP:rs61752427)
1314 P → T: in dbSNP:rs61754041
1332:2273 natural variant: Missing (in STGD1; uncertain significance)
1357 A→T: Decreases solubility at 50%. Loss of intracellular vesicle localization. Does not affect substrate binding. Reduces basal ATPase activity.
1368 R → C: in CORD3; uncertain significance; dbSNP:rs1183074086
1371 K → N: in STGD1; uncertain significance
1380 P → L: in STGD1; also found in a patient with chorioretinal atrophy; reduced ATP-binding capacity; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; dbSNP:rs61750130
1399 E → K: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; increases N-Ret-PE binding; dbSNP:rs62642573
1408 W → L: in STGD1; does not affect secondary structure; decreases structural flexibility; significantly decreases all-trans-retinal binding; dbSNP:rs61750134; W → R: in STGD1; reduced retinal-stimulated ATP hydrolysis; dbSNP:rs61750135
1408:2273 natural variant: Missing (in STGD1; uncertain significance)
1416 natural variant: Missing (in STGD1; uncertain significance)
1428 T → M: in dbSNP:rs1800549
1442 N → K: in STGD1; uncertain significance; dbSNP:rs762150575
1443 R → H: in STGD1; loss of the majority of alpha-helical secondary structure; does not bind all-trans-retinal; does not affect conformational change; dbSNP:rs61750142
1444 modified: Disulfide link with 1455
1453:2273 natural variant: Missing (in STGD1; uncertain significance)
1455 modified: Disulfide link with 1444
1461:2273 natural variant: Missing (in STGD1; uncertain significance)
1469 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1479:2273 natural variant: Missing (in STGD1 and CORD3; uncertain significance; dbSNP:rs61752434)
1484 P → S: in STGD1; uncertain significance; dbSNP:rs1660139125
1488 modified: Disulfide link with 1502; C → R: in STGD1 and FFM; also found in a patient with chorioretinal atrophy; reduced retinal-stimulated ATP hydrolysis; does not affect secondary structure; oss of structural flexibility; significantly decreases all-trans-retinal binding; dbSNP:rs61750146
1490 modified: Disulfide link with 641, Interchain; C → Y: in STGD1 and CORD3; reduced retinal-stimulated ATP hydrolysis; dbSNP:rs61751402
1502 modified: Disulfide link with 1488; C→R: Moderately decreased protein abundance. Moderately decreased ATPase activity. Moderately decreased phospholipid translocase activity.
1503 P → L: in STGD1; uncertain significance
1511 P → H: in STGD1; uncertain significance; dbSNP:rs886046564
1512 P → R: in STGD1; uncertain significance; dbSNP:rs61750150
1526 T → M: in STGD1; also found in a patient with chorioretinal atrophy; reduced retinal-stimulated ATP hydrolysis; dbSNP:rs61750152
1529 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1537 T → M: in STGD1; moderately decreased protein abundance; moderately decreased ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs62642575
1551 natural variant: Missing (in STGD1; uncertain significance)
1556 R → T: in STGD1; uncertain significance; dbSNP:rs1385119665
1557 Y → C: found in a patient with chorioretinal atrophy; uncertain significance; dbSNP:rs1401716074
1562 I → T: in STGD1, FFM, ARMD2 and CORD3; uncertain significance; dbSNP:rs1762111
1572 T → M: in dbSNP:rs185093512
1588 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1591 G → R: in STGD1; uncertain significance; also found in a patient with macular dystrophy; uncertain significance; dbSNP:rs113106943
1598 A → D: in CORD3 and STGD1; uncertain significance; dbSNP:rs61750155
1618:2273 natural variant: Missing
1623 G → V: in dbSNP:rs1571257969
1637 A → T: in dbSNP:rs61754056
1640 R → Q: in STGD1, FFM and CORD3; dbSNP:rs61751403; R → W: in STGD1 and CORD3; dbSNP:rs61751404
1650:2273 natural variant: Missing (in CORD3; uncertain significance)
1652:2273 natural variant: Missing (in STGD1 and FFM; uncertain significance)
1662 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1681:1685 natural variant: Missing (in STGD1; highly reduced ATP-binding capacity)
1696 S → N: in STGD1; uncertain significance; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; increases N-Ret-PE binding; dbSNP:rs61750564
1703 Q → E: in STGD1; uncertain significance; does not affect solubility; does not affect location in cytoplasmic vesicle;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; dbSNP:rs61750565; Q → K: in STGD1; moderately decreases solubility; loss of cytoplasmic vesicle localization;decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity
1705 R → L: in STGD1; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; dbSNP:rs61753021; R → Q: in STGD1; uncertain significance; dbSNP:rs61753021
1724:2273 natural variant: Missing (in STGD1; uncertain significance)
1754 Y → D: in STGD1; uncertain significance
1761:1763 natural variant: Missing (in STGD1; highly reduced ATP-binding capacity)
1762 A → D: in STGD1; uncertain significance; dbSNP:rs121909206
1773 A → E: found in a patient with chorioretinal atrophy; uncertain significance; severely decreases solubility; loss of cytoplasmic vesicle localization; decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; dbSNP:rs760549861; A → V: in STGD1; uncertain significance; severely decreases solubility; loss of cytoplasmic vesicle localization; decreases basal ATPase activity below 50%; loss of N-Ret-PE-induced stimulation in ATPase activity; dbSNP:rs760549861
1775 I → N: in STGD1; uncertain significance; dbSNP:rs771742619
1779 Y → H: in STGD1; uncertain significance
1779:2273 natural variant: Missing (in STGD1; uncertain significance)
1794 A → D: in STGD1; also found in a patient with bull's eye maculopathy; uncertain significance; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases basal ATPase activity below 50%; severely decreases N-Ret-PE-stimulated ATPase activity; dbSNP:rs61751406; A → P: in STGD1; uncertain significance; loss of cytoplasmic vesicle localization; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; decreases solubility below 50%; significantly reduces N-Ret-PE binding in the absence of ATP; dbSNP:rs1571252997
1805 N → D: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; dbSNP:rs61753029
1817 E → D: in dbSNP:rs1029950404
1838 H → D: in STGD1; uncertain significance; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases basal ATPase activity below 50%; decreases modestly N-Ret-PE-stimulated ATPase; dbSNP:rs62642562; H → N: in STGD1; uncertain significance; does not affect solubility; does not affect location in cytoplasmic vesicle; decreases basal ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; dbSNP:rs62642562; H → Y: in STGD1; moderately decreases solubility; loss of cytoplasmic vesicle localization; decreases basal ATPase activity below 50%; severely decreases N-Ret-PE-stimulated ATPase activity; dbSNP:rs62642562; H→R: Severely decreases solubility. Loss of cytoplasmic vesicle localization. Decreases basal ATPase activity below 50%. Loss of N-Ret-PE-induced stimulation in ATPase activity.
1843 R → W: in STGD1; does not affect solubility; does not affect location in cytoplasmic vesicle; decreases ATPase activity between 50% and 80%; decreases modestly N-Ret-PE-stimulated ATPase; does not affect N-Ret-PE binding; dbSNP:rs62642576
1868 N → I: risk factor for STGD1; slightly reduced retinal-stimulated ATP hydrolysis; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; does not affect N-Ret-PE binding; dbSNP:rs1801466
1882 M → I: in CORD3; uncertain significance; dbSNP:rs752160946
1886 G → E: in STGD1; highly reduced ATP-binding capacity; dbSNP:rs62642579
1898 R → C: does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; dbSNP:rs201357151; R → H: in STGD1 and ARMD2; does not affect solubility; does not affect location in cytoplasmic vesicle; does not affect both basal and N-Ret-PE-stimulated ATPase activity; Increases N-Ret-PE binding; dbSNP:rs1800552
1921 V → G: in STGD1; uncertain significance
2030:2273 natural variant: Missing (in STGD1 and CORD3; uncertain significance; dbSNP:rs61751383)
2040:2273 natural variant: Missing (in STGD1; uncertain significance; also found in a patient with chorioretinal atrophy; uncertain significance; dbSNP:rs61753038)
2255 S → I: in dbSNP:rs6666652

7w02A Cryo-em structure of atp-bound abca3 (see paper)
40% identity, 64% coverage: 17:226/329 of query aligns to 512:717/1566 of 7w02A

query
sites
7w02A

T

A

A

A

V

V

N

R

S

D

L

L

N

N

L

L

A

N

I

L

H

Y

K

E

G

G

T

Q

I

I

F

T

G

V

L

L

G

G

P

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

S

S

M

M

L

L

C

T

T

G

I

L

L

F

N

P

P

P

T

T

S

S

G

G

T

R

A

A

T

Y

V

I

N

S

G

G

Y

Y

D

E

I

I

L

S

T

Q

E

D

S

M

E

V

K

Q

V

I

R

R

Q

K

S

S

I

L

G

G

I

L

V

C

F

P

Q
|
Q

S

H

I

D

S

I

I

L

D

F

D

D

R

N

L

L

T

T

G

V

R

A

E

E

N

H

L

L

K

Y

F

F

H

Y

A

A

M

Q

L

L

Y

K

N

G

V

L

P

S

E

R

D

Q

K

K

I

C

A

P

G

E

R

E

I

V

D

K

E

Q

V

M

L

L

A

H

L

I

E

G

L

L

S

E

D

D

R

K

A

W

D

N

D

S

L

R

V

S

R

R

T

F

Y
x
L

S
|
S

G

G

M

M

I

R

R

R

R

K

L

L

E

S

M

I

A

G

R

I

G

A

L

L

L

I

H

A

H

G

P

S

H

K

I

V

L

L

F

I

L

L

D

D

E

Q

P

P

T

T

I

S

G

G

L

M

D

D

P

A

Q

I

T

S

R

R

E

R

H

A

I

I

W

W

S

-

Y

-

I

-

Q

-

E

D

L

L

T

L

R

Q

K

R

K

Q

K

K

G

S

D

D

L

R

T

T

V

I

L

V

L

L

T

T

H

H

Y

F

M

M

D

D

E

E

A

A

D

D

L

L

C

G

D

D

E

R

V

I

A

A

I

I

I

M

D

A

K

K

G

G

Q

E

I

L

I

Q

V

C

R

C

D

G

T

S

P

S

G

L

N

F

L

L

K

K

Q

Q

binding adenosine-5'-triphosphate: N533 (= N38), G534 (= G39), G536 (= G41), K537 (= K42), T538 (≠ S43), T539 (= T44), Q578 (= Q83), L630 (≠ Y135), S631 (= S136)
binding magnesium ion: T538 (≠ S43), Q578 (= Q83)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 504, 1277, 1305, 1306, 1307, 1308, 1309, 1310, 1344, 1395, 1398
binding magnesium ion: 1310, 1344

8f5bA Human abca4 structure in complex with amp-pnp (see paper)
34% identity, 89% coverage: 18:309/329 of query aligns to 1632:1923/1924 of 8f5bA

query
sites
8f5bA

A

A

V

V

N

D

S

R

L

L

N

C

L

V

A

G

I

V

H

R

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

S
x
T

T

T

L

T

L

F

S

K

M

M

L

L

C

T

T

G

I

D

L

T

N

T

P

V

T

T

S

S

G

G

T

D

A

A

T

T

V

V

N

A

G

G

Y

K

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S

I

I

L

L

T

T

E

N

S

I

E

S

K

E

V

V

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Q

Q

S

N

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G

G

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Q

S

F

I

D

S

A

I

I

D

D

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

Y

F

L

H

Y

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

A

D

E

K

E

I

I

A

E

G

K

R

V

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A

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N

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W

V

S

L

I

A

K

L

S

L

L

E

G

L

L

S

T

D

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R

Y

A

A

D

D

D

C

L

L

V

A

R

G

T
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T

Y

Y

S
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S

G

G

M

N

I

K

R

R

R

K

L

L

E

S

M

T

A

A

R

I

G

A

L

L

L

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H

G

H

C

P

P

H

P

I

L

L

V

F

L

L

L

D

D

E

E

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

E

R

H

M

I

L

W

W

S

N

Y

V

I

I

Q

V

E

S

L

I

T

I

R

R

K

E

K

G

K

R

G

A

D

-

L

-

T

-

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

T

E

R

V

L

A

A

I

I

I

M

D

V

K

K

G

G

Q

A

I

F

I

R

V

C

R

M

D

G

T

T

P

I

G

Q

N

H

L

L

K

K

Q

S

D

K

L

F

-

G

Q

D

G

G

E

Y

K

I

I

V

S

T

F

M

N

K

M

I

K

K

S

S

P

D

-

L

-

N

E

P

I

V

L

E

A

Q

S

F

L

F

L

Q

S

G

D

N

N

F

P

P

R

G

I

S

C

V

K

Q

V

R

S

E

T

R

E

H

S

Y

G

N

L

M

L

L

E

Q

V

F

Q

Q

V

V

I

-

H

-

N

S

G

S

N

S

I

S

V

L

Q

A

E

R

I

I

F

F

D

Q

-

L

I

L

A

S

R

H

E

K

A

D

G

S

I

L

E

L

I

I

D

E

H

E

I

Y

S

S

I

V

H

T

E

Q

S

T

M

L

H

D

D

Q

V

V

F

F

L

V

H

N

Y

F

T

A

G

K

K

Q

E

Q

binding phosphoaminophosphonic acid-adenylate ester: N1652 (= N38), G1653 (= G39), A1654 (= A40), G1655 (= G41), K1656 (= K42), T1657 (≠ S43), Q1697 (= Q83), T1748 (= T134), S1750 (= S136)
binding magnesium ion: T1657 (≠ S43), Q1697 (= Q83)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 757, 758, 760, 761, 762, 763, 802, 853
binding magnesium ion: 762, 802

7lkzA Structure of atp-bound human abca4 (see paper)
34% identity, 89% coverage: 18:309/329 of query aligns to 1626:1920/1920 of 7lkzA

query
sites
7lkzA

A

A

V

V

N

D

S

R

L

L

N

C

L

V

A

G

I

V

H

R

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

S

K

M

M

L

L

C

T

T

G

I

D

L

T

N

T

P

V

T

T

S

S

G

G

T

D

A

A

T

T

V

V

N

A

G

G

Y

K

D

S

I

I

L

L

T

T

E

N

S

I

E

S

K

E

V

V

R

H

Q

Q

S

N

I

M

G

G

I

Y

V

C

F

P

Q

Q

S

F

I

D

S

A

I

I

D

D

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

Y

F

L

H

Y

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

A

D

E

K

E

I

I

A

E

G

K

R

V

I

A

D

N

E

W

V

S

L

I

A

K

L

S

L

L

E

G

L

L

S

T

D

V

R

Y

A

A

D

D

D

C

L

L

V

A

R

G

T
|
T

Y

Y

S
|
S

G
|
G

G

G

M

N

I

K

R

R

R

K

L

L

E

S

M

T

A

A

R

I

G

A

L

L

L

I

H

G

H

C

P

P

H

P

I

L

L

V

F

L

L

L

D

D

E

Q

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

E

R

H

M

I

L

W

W

S

N

Y

V

I

I

Q

V

E

S

L

I

T

I

R

R

K

E

K

G

K

R

G

A

D

-

L

-

T

-

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

T

E

R

V

L

A

A

I

I

I

M

D

V

K

K

G

G

Q

A

I

F

I

R

V

C

R

M

D

G

T

T

P

I

G

Q

N

H

L

L

K

K

Q

S

D

K

L

F

Q

-

G

G

E

D

K

G

I

Y

S

I

F

V

N

T

M

M

K

K

S

I

P

K

E

S

I

L

L

L

A

P

S

D

L

L

-

N

-

P

-

V

-

E

-

Q

-

F

L

Q

S

G

D

N

N

F

P

P

R

G

I

S

C

V

K

Q

V

R

S

E

T

R

E

H

S

Y

G

N

L

M

L

L

E

Q

V

F

Q

Q

V

V

I

-

H

-

N

S

G

S

N

S

I

S

V

L

Q

A

E

R

I

I

F

F

D

Q

-

L

I

L

A

S

R

H

E

K

A

D

G

S

I

L

E

L

I

I

D

E

H

E

I

Y

S

S

I

V

H

T

E

Q

S

T

M

L

H

D

D

Q

V

V

F

F

L

V

H

N

Y

F

T

A

G

K

K

Q

E

Q

binding adenosine-5'-triphosphate: N1646 (= N38), G1647 (= G39), G1649 (= G41), K1650 (= K42), T1651 (≠ S43), T1652 (= T44), T1742 (= T134), S1744 (= S136), G1745 (= G137)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 22, 465, 566, 567, 568, 572, 592, 593, 595, 1076, 1355, 1362, 1470, 1471
binding adenosine-5'-triphosphate: 718, 725, 745, 746, 748, 749, 750, 834, 841, 843, 1619
binding magnesium ion: 750, 790

7e7qA Cryo-em structure of human abca4 in atp-bound state (see paper)
34% identity, 89% coverage: 18:309/329 of query aligns to 1678:1958/1958 of 7e7qA

query
sites
7e7qA

A

A

V

V

N

D

S

R

L

L

N

C

L

V

A

G

I

V

H

R

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

S

K

M

M

L

L

C

T

T

G

I

D

L

T

N

T

P

V

T

T

S

S

G

G

T

D

A

A

T

T

V

V

N

A

G

G

Y

K

D

S

I

I

L

L

T

T

E

N

S

I

E

S

K

E

V

V

R

H

Q

Q

S

N

I

M

G

G

I

Y

V

C

F

P

Q
|
Q

S

F

I

D

S

A

I

I

D

D

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

Y

F

L

H

Y

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

A

D

E

K

E

I

I

A

E

G

K

R

V

I

A

D

N

E

W

V

S

L

I

A

K

L

S

L

L

E

G

L

L

S

T

D

V

R

Y

A

A

D

D

D

C

L

L

V

A

R

G

T
|
T

Y

Y

S
|
S

G

G

M

N

I

K

R

R

R

K

L

L

E

S

M

T

A

A

R

I

G

A

L

L

L

I

H

G

H

C

P

P

H

P

I

L

L

V

F

L

L

L

D

D

E

Q

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

E

R

H

M

I

L

W

W

S

N

Y

V

I

I

Q

V

E

S

L

I

T

I

R

R

K

E

K

G

K

R

G

A

D

-

L

-

T

-

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

T

E

R

V

L

A

A

I

I

I

M

D

V

K

K

G

G

Q

A

I

F

I

R

V

C

R

M

D

G

T

T

P

I

G

Q

N

H

L

L

K

K

Q

S

D

K

L

F

Q

-

G

G

E

D

K

G

I

Y

S

I

F

V

N

T

M

M

K

K

S

I

P

N

E

P

I

V

L

E

A

Q

S

F

L

F

L

Q

S

G

D

N

N

F

P

P

R

G

I

S

C

V

K

Q

V

R

S

E

T

R

E

H

S

Y

G

N

L

M

L

L

E

Q

V

F

Q

Q

V

V

I

-

H

-

N

S

G

S

N

S

I

S

V

L

Q

A

E

R

I

I

F

F

D

Q

I

L

A

L

R

L

E

S

A

-

G

-

I

-

E

-

I

-

D

E

H

E

I

Y

S

S

I

V

H

T

E

Q

S

T

M

L

H

D

D

Q

V

V

F

F

L

V

H

N

Y

F

T

A

G

K

K

Q

E

Q

binding adenosine-5'-triphosphate: N1698 (= N38), G1701 (= G41), K1702 (= K42), T1703 (≠ S43), T1704 (= T44), Q1743 (= Q83), T1794 (= T134), S1796 (= S136)
binding magnesium ion: T1703 (≠ S43), Q1743 (= Q83)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 801, 804, 808, 828, 831, 832, 833, 834, 873, 926, 928, 1671
binding magnesium ion: 833, 873

7lkpA Structure of atp-free human abca4 (see paper)
34% identity, 89% coverage: 18:309/329 of query aligns to 1647:1940/1941 of 7lkpA

query
sites
7lkpA

A

A

V

V

N

D

S

R

L

L

N

C

L

V

A

G

I

V

H

R

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

L

F

S

K

M

M

L

L

C

T

T

G

I

D

L

T

N

T

P

V

T

T

S

S

G

G

T

D

A

A

T

T

V

V

N

A

G

G

Y

K

D

S

I

I

L

L

T

T

E

N

S

I

E

S

K

E

V

V

R

H

Q

Q

S

N

I

M

G

G

I

Y

V

C

F

P

Q

Q

S

F

I

D

S

A

I

I

D

D

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

Y

F

L

H

Y

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

A

D

E

K

E

I

I

A

E

G

K

R

V

I

A

D

N

E

W

V

S

L

I

A

K

L

S

L

L

E

G

L

L

S

T

D

V

R

Y

A

A

D

D

D

C

L

L

V

A

R

G

T

T

Y

Y

S

S

G

G

M

N

I

K

R

R

R

K

L

L

E

S

M

T

A

A

R

I

G

A

L

L

L

I

H

G

H

C

P

P

H

P

I

L

L

V

F

L

L

L

D

D

E

E

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

E

R

H

M

I

L

W

W

S

N

Y

V

I

I

Q

V

E

S

L

I

T

I

R

R

K

E

K

G

K

R

G

A

D

-

L

-

T

-

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

T

E

R

V

L

A

A

I

I

I

M

D

V

K

K

G

G

Q

A

I

F

I

R

V

C

R

M

D

G

T

T

P

I

G

Q

N

H

L

L

K

K

Q

S

D

K

L

F

-

G

Q

D

G

G

E

Y

K

I

I

V

S

T

F

M

N

K

M

I

K

K

-

L

S

L

P

P

E

D

I

L

-

N

-

P

L

V

A

E

S

Q

L

F

S

Q

D

G

N

N

-

F

P

P

R

G

I

S

C

V

K

Q

V

R

S

E

T

R

E

H

S

Y

G

N

L

M

L

L

E

Q

V

F

Q

Q

V

V

I

-

H

-

N

S

G

S

N

S

I

S

V

L

Q

A

E

R

I

I

F

F

D

Q

-

L

I

L

A

S

R

H

E

K

A

D

G

S

I

L

E

L

I

I

D

E

H

E

I

Y

S

S

I

V

H

T

E

Q

S

T

M

L

H

D

D

Q

V

V

F

F

L

V

H

N

Y

F

T

A

G

K

K

Q

E

Q

Sites not aligning to the query:

binding Digitonin: 522, 526, 530, 533, 616, 819, 863
binding cholesterol: 9, 10, 13, 617, 1073, 1074, 1076, 1402, 1436, 1487, 1492, 1495, 1496, 1499, 1603

7e7oA Cryo-em structure of human abca4 in nrpe-bound state (see paper)
41% identity, 64% coverage: 18:228/329 of query aligns to 1719:1926/2003 of 7e7oA

query
sites
7e7oA

A

A

V

V

N

D

S

R

L

L

N

C

L

V

A

G

I

V

H

R

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

L

F

S

K

M

M

L

L

C

T

T

G

I

D

L

T

N

T

P

V

T

T

S

S

G

G

T

D

A

A

T

T

V

V

N

A

G

G

Y

K

D

S

I

I

L

L

T

T

E

N

S

I

E

S

K

E

V

V

R

H

Q

Q

S

N

I

M

G

G

I

Y

V

C

F

P

Q

Q

S

F

I

D

S

A

I

I

D

D

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

Y

F

L

H

Y

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

A

D

E

K

E

I

I

A

E

G

K

R

V

I

A

D

N

E

W

V

S

L

I

A

K

L

S

L

L

E

G

L

L

S

T

D

V

R

Y

A

A

D

D

D

C

L

L

V

A

R

G

T

T

Y

Y

S

S

G

G

M

N

I

K

R

R

R

K

L

L

E

S

M

T

A

A

R

I

G

A

L

L

L

I

H

G

H

C

P

P

H

P

I

L

L

V

F

L

L

L

D

D

E

E

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

E

R

H

M

I

L

W

W

S

N

Y

V

I

I

Q

V

E

S

L

I

T

I

R

R

K

E

K

G

K

R

G

A

D

-

L

-

T

-

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

T

E

R

V

L

A

A

I

I

I

M

D

V

K

K

G

G

Q

A

I

F

I

R

V

C

R

M

D

G

T

T

P

I

G

Q

N

H

L

L

K

K

Q

S

D

K

L

F

Sites not aligning to the query:

binding [(2S)-3-[2-[(E)-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenylidene]amino]ethoxy-oxidanyl-phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxy-propyl] (Z)-octadec-9-enoate: 285, 290, 526, 527, 589, 592, 1454

8ee6A Cryo-em structure of human abca7 in pe/ch nanodiscs (see paper)
33% identity, 91% coverage: 8:306/329 of query aligns to 1502:1806/1808 of 8ee6A

query
sites
8ee6A

N

N

L

L

T

T

K

K

Q

V

F
x
Y

-

R

-

G

G

Q

T

R

L

M

T

P

A
|
A

V

V

N

D

S

R

L

L

N

C

L

L

A

G

I

I

H

P

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N

N

G
|
G

A
|
A

G
|
G

K
|
K

S
x
T

T

S

L

T

L

F

S

R

M

M

L

V

C

T

T

G

I

D

L

T

N

L

P

A

T

S

S

R

G

G

T

E

A

A

T

V

V

L

N

A

G

G

Y

H

D

S

I

V

L

A

T

R

E

E

S

P

E

S

K

A

V

A

R

H

Q

L

S

S

I

M

G

G

I

Y

V

C

F

P

Q

Q

S

S

I

D

S

A

I

I

D

F

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

E

F

L

H

L

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

E

D

A

K

Q

I

V

A

A

G

Q

R

T

I

A

D

G

E

S

V

G

L

L

A

A

L

R

L

L

E

G

L

L

S

S

D

W

R

Y

A

A

D

D

D

R

L

P

V

A

R

G

T

T

Y

Y

S

S

G

G

M

N

I

K

R

R

R

K

L

L

E

A

M

T

A

A

R

L

G

A

L

L

L

V

H

G

H

D

P

P

H

A

I

V

L

V

F

F

L

L

D

D

E

E

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

S

T

A

R

R

E

R

H

F

I

L

W

W

S

N

Y

S

I

L

Q

L

E

A

L

V

T

V

R

R

K

E

K

G

K

R

G

-

D

-

L

-

T

S

V

V

L

M

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

S

E

R

V

L

A

A

I

I

I

M

D

V

K

N

G

G

Q

R

I

F

I

R

V

C

R

L

D

G

T

S

P

P

G

Q

N

H

L

L

K

K

Q

G

D

R

L

F

-

A

Q

A

G

G

E

H

K

T

I

L

S

T

F

L

N

R

M

V

-

P

-

A

K

R

S

S

P

Q

E

P

I

A

L

A

A

A

S

F

L

V

L

A

S

A

D

E

N

F

P

P

R

G

I

A

C

E

K

L

V

R

S

E

T

A

E

H

S

G

G

G

L

R

L

L

E

R

V

F

Q

Q

V

L

I

P

H

P

N

G

G

G

-

R

-

C

-

A

-

L

-

A

N

R

I

V

V

F

Q

G

E

E

I

L

F

A

D

V

I

H

A

G

R

A

E

E

A

H

G

G

I

V

E

E

I

-

D

-

H

D

I

F

S

S

I

V

H

S

E

Q

S

T

S

M

M

L

H

E

D

E

V

V

F

F

L

L

H

Y

Y

F

T

S

binding phosphothiophosphoric acid-adenylate ester: Y1507 (≠ F13), A1514 (= A18), G1535 (= G39), A1536 (= A40), G1537 (= G41), K1538 (= K42), T1539 (≠ S43)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 607, 608, 633, 640, 660, 661, 663, 664, 665, 666

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 66% coverage: 4:220/329 of query aligns to 2:224/343 of P30750

query
sites
P30750

I

I

Q

K

T

L

Y

S

N

N

L

I

T

T

K

K

Q

V

F

F

G

H

-

Q

-

G

-

T

-

R

T

T

L

I

T

Q

A

A

V

L

N

N

S

N

L

V

N

S

L

L

A

H

I

V

H

P

K

A

G

G

T

Q

I

I

F

Y

G

G

L

V

L

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

S

R

M

C

L

V

C

N

T

L

I

L

L

E

N

R

P

P

T

T

S

E

G

G

T

S

A

V

T

L

V

V

N

D

G

G

Y

Q

D

E

I

L

L

T

T

T

E

L

S

S

E

E

-

S

-

E

-

L

-

T

K

K

V

A

R

R

Q

R

S

Q

I

I

G

G

I

M

V

I

F

F

Q

Q

S

H

I

F

S

N

I

L

D

L

D

S

R

S

L

R

T

T

G

V

R

F

E

G

N

N

L

V

K

A

F

L

H

P

A

L

M

E

L

L

Y

D

N

N

V

T

P

P

E

K

D

D

K

E

I

V

A

K

G

R

R

R

I

V

D

T

E

E

V

L

L

L

A

S

L

L

L

V

E

G

L

L

S

G

D

D

R

K

A

H

D

D

D

S

L

Y

V

P

R

S

T

N

Y

L

S

S

G

G

M

Q

I

K

R

Q

R

R

L

V

E

A

M

I

A

A

R

R

G

A

L

L

L

A

H

S

H

N

P

P

H

K

I

V

L

L

F

L

L

C

D

D

E
|
E

P

A

T

T

I

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

E

R

H

S

I

I

W

L

S

E

Y

L

I

L

Q

K

E

D

L

I

T

N

R

R

K

R

K

L

K

-

G

-

D

G

L

L

T

T

V

I

L

L

T

I

T

T

H

H

Y

E

M

M

D

D

E

V

A

V

D

K

L

R

L

I

C

C

D

D

E

C

V

V

A

A

I

V

I

I

D

S

K

N

G

G

Q

E

I

L

I

I

V

E

R

Q

D

D

T

T

40:46 (vs. 38:44, 86% identical) binding ATP
E166 (= E160) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
36% identity, 66% coverage: 4:220/329 of query aligns to 3:225/344 of 6cvlD

query
sites
6cvlD

I

I

Q

K

T

L

Y

S

N

N

L

I

T

T

K

K

Q

V

F
|
F

G

H

-
x
Q

-

G

-

T

-

R

T

T

L
x
I

T

Q

A

A

V

L

N

N

S

N

L

V

N

S

L

L

A

H

I

V

H

P

K

A

G

G

T

Q

I

I

F

Y

G

G

L

V

L

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

S

R

M

C

L

V

C

N

T

L

I

L

L

E

N

R

P

P

T

T

S

E

G

G

T

S

A

V

T

L

V

V

N

D

G

G

Y

Q

D

E

I

L

L

T

T

T

E

L

S

S

E

E

-

S

-

E

-

L

-

T

K

K

V

A

R

R

Q

R

S

Q

I

I

G

G

I

M

V

I

F

F

Q

Q

S

H

I

F

S

N

I

L

D

L

D

S

R

S

L

R

T

T

G

V

R

F

E

G

N

N

L

V

K

A

F

L

H

P

A

L

M

E

L

L

Y

D

N

N

V

T

P

P

E

K

D

D

K

E

I

V

A

K

G

R

R

R

I

V

D

T

E

E

V

L

L

L

A

S

L

L

L

V

E

G

L

L

S

G

D

D

R

K

A

H

D

D

D

S

L

Y

V

P

R

S

T
x
N

Y

L

S
|
S

G

G

M
x
Q

I

K

R

Q

R

R

L

V

E

A

M

I

A

A

R

R

G

A

L

L

L

A

H

S

H

N

P

P

H

K

I

V

L

L

F

L

L

C

D

D

E

Q

P

A

T

T

I

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

E

R

H

S

I

I

W

L

S

E

Y

L

I

L

Q

K

E

D

L

I

T

N

R

R

K

R

K

L

K

-

G

-

D

G

L

L

T

T

V

I

L

L

T

I

T

T

H
|
H

Y

E

M

M

D

D

E

V

A

V

D

K

L

R

L

I

C

C

D

D

E

C

V

V

A

A

I

V

I

I

D

S

K

N

G

G

Q

E

I

L

I

I

V

E

R

Q

D

D

T

T

binding phosphothiophosphoric acid-adenylate ester: F12 (= F13), Q14 (vs. gap), I19 (≠ L16), S41 (≠ N38), G42 (= G39), A43 (= A40), G44 (= G41), K45 (= K42), S46 (= S43), T47 (= T44), N141 (≠ T134), S143 (= S136), Q146 (≠ M139), H200 (= H195)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
36% identity, 66% coverage: 4:220/329 of query aligns to 3:225/344 of 3tuzC

query
sites
3tuzC

I

I

Q

K

T

L

Y

S

N

N

L

I

T

T

K

K

Q

V

F
|
F

G

H

-
x
Q

-

G

-

T

-

R

T

T

L

I

T

Q

A

A

V

L

N

N

S

N

L

V

N

S

L

L

A

H

I

V

H

P

K

A

G

G

T

Q

I

I

F

Y

G

G

L

V

L

I

G

G

P

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

S

R

M

C

L

V

C

N

T

L

I

L

L

E

N

R

P

P

T

T

S

E

G

G

T

S

A

V

T

L

V

V

N

D

G

G

Y

Q

D

E

I

L

L

T

T

T

E

L

S

S

E

E

-

S

-

E

-

L

-

T

K

K

V

A

R

R

Q

R

S

Q

I

I

G

G

I

M

V

I

F

F

Q

Q

S

H

I

F

S

N

I

L

D

L

D

S

R

S

L

R

T

T

G

V

R

F

E

G

N

N

L

V

K

A

F

L

H

P

A

L

M

E

L

L

Y

D

N

N

V

T

P

P

E

K

D

D

K

E

I

V

A

K

G

R

R

R

I

V

D

T

E

E

V

L

L

L

A

S

L

L

L

V

E

G

L

L

S

G

D

D

R

K

A

H

D

D

D

S

L

Y

V

P

R

S

T

N

Y

L

S

S

G

G

M

Q

I

K

R

Q

R

R

L

V

E

A

M

I

A

A

R

R

G

A

L

L

L

A

H

S

H

N

P

P

H

K

I

V

L

L

F

L

L

C

D

D

E

Q

P

A

T

T

I

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

E

R

H

S

I

I

W

L

S

E

Y

L

I

L

Q

K

E

D

L

I

T

N

R

R

K

R

K

L

K

-

G

-

D

G

L

L

T

T

V

I

L

L

T

I

T

T

H

H

Y

E

M

M

D

D

E

V

A

V

D

K

L

R

L

I

C

C

D

D

E

C

V

V

A

A

I

V

I

I

D

S

K

N

G

G

Q

E

I

L

I

I

V

E

R

Q

D

D

T

T

binding adenosine-5'-diphosphate: F12 (= F13), Q14 (vs. gap), G42 (= G39), G44 (= G41), K45 (= K42), S46 (= S43), T47 (= T44)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
36% identity, 66% coverage: 4:220/329 of query aligns to 3:225/344 of 3tuiC

query
sites
3tuiC

I

I

Q

K

T

L

Y

S

N

N

L

I

T

T

K

K

Q

V

F
|
F

G

H

-

Q

-

G

-

T

-

R

T

T

L

I

T

Q

A

A

V

L

N

N

S

N

L

V

N

S

L

L

A

H

I

V

H

P

K

A

G

G

T

Q

I

I

F

Y

G

G

L

V

L

I

G

G

P

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

S

R

M

C

L

V

C

N

T

L

I

L

L

E

N

R

P

P

T

T

S

E

G

G

T

S

A

V

T

L

V

V

N

D

G

G

Y

Q

D

E

I

L

L

T

T

T

E

L

S

S

E

E

-

S

-

E

-

L

-

T

K

K

V

A

R

R

Q

R

S

Q

I

I

G

G

I

M

V

I

F

F

Q

Q

S

H

I

F

S

N

I

L

D

L

D

S

R

S

L

R

T

T

G

V

R

F

E

G

N

N

L

V

K

A

F

L

H

P

A

L

M

E

L

L

Y

D

N

N

V

T

P

P

E

K

D

D

K

E

I

V

A

K

G

R

R

R

I

V

D

T

E

E

V

L

L

L

A

S

L

L

L

V

E

G

L

L

S

G

D

D

R

K

A

H

D

D

D

S

L

Y

V

P

R

S

T

N

Y

L

S

S

G

G

M

Q

I

K

R

Q

R

R

L

V

E

A

M

I

A

A

R

R

G

A

L

L

L

A

H

S

H

N

P

P

H

K

I

V

L

L

F

L

L

C

D

D

E

Q

P

A

T

T

I

S

G

A

L

L

D

D

P

P

Q

A

T

T

R

T

E

R

H

S

I

I

W

L

S

E

Y

L

I

L

Q

K

E

D

L

I

T

N

R

R

K

R

K

L

K

-

G

-

D

G

L

L

T

T

V

I

L

L

T

I

T

T

H

H

Y

E

M

M

D

D

E

V

A

V

D

K

L

R

L

I

C

C

D

D

E

C

V

V

A

A

I

V

I

I

D

S

K

N

G

G

Q

E

I

L

I

I

V

E

R

Q

D

D

T

T

binding adenosine-5'-diphosphate: F12 (= F13), G42 (= G39), A43 (= A40), G44 (= G41), K45 (= K42), S46 (= S43), T47 (= T44)

8eopA Cryo-em structure of nanodisc reconstituted human abca7 eq mutant in atp bound closed state (see paper)
38% identity, 66% coverage: 8:225/329 of query aligns to 1456:1672/1687 of 8eopA

query
sites
8eopA

N

N

L

L

T

T

K

K

Q

V

F
x
Y

-

R

-

G

G

Q

T

R

L

M

T

P

A

A

V

V

N

D

S

R

L

L

N

C

L

L

A

G

I

I

H

P

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
x
S

L

T

L

F

S

R

M

M

L

V

C

T

T

G

I

D

L

T

N

L

P

A

T

S

S

R

G

G

T

E

A

A

T

V

V

L

N

A

G

G

Y

H

D

S

I

V

L

A

T

R

E

E

S

P

E

S

K

A

V

A

R

H

Q

L

S

S

I

M

G

G

I

Y

V

C

F

P

Q
|
Q

S

S

I

D

S

A

I

I

D

F

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

E

F

L

H

L

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

E

D

A

K

Q

I

V

A

A

G

Q

R

T

I

A

D

G

E

S

V

G

L

L

A

A

L

R

L

L

E

G

L

L

S

S

D

W

R

Y

A

A

D

D

D

R

L

P

V

A

R

G

T
|
T

Y

Y

S
|
S

G

G

M

N

I

K

R

R

R

K

L

L

E

A

M

T

A

A

R

L

G

A

L

L

L

V

H

G

H

D

P

P

H

A

I

V

L

V

F

F

L

L

D

D

E

Q

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

S

T

A

R

R

E

R

H

F

I

L

W

W

S

N

Y

S

I

L

Q

L

E

A

L

V

T

V

R

R

K

E

K

G

K

R

G

-

D

-

L

-

T

S

V

V

L

M

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

S

E

R

V

L

A

A

I

I

I

M

D

V

K

N

G

G

Q

R

I

F

I

R

V

C

R

L

D

G

T

S

P

P

G

Q

N

H

L

L

K

K

binding adenosine-5'-triphosphate: Y1461 (≠ F13), N1488 (= N38), G1489 (= G39), G1491 (= G41), K1492 (= K42), S1494 (≠ T44), Q1533 (= Q83), T1584 (= T134), S1586 (= S136)
binding magnesium ion: T1493 (≠ S43), Q1533 (= Q83)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 659, 662, 666, 686, 687, 689, 690, 691, 692, 782, 784

7m1qA Human abca4 structure in complex with n-ret-pe (see paper)
39% identity, 64% coverage: 26:237/329 of query aligns to 1659:1877/1911 of 7m1qA

query
sites
7m1qA

I

V

H

R

K

P

G

G

T

E

I

C

F

F

G

G

L

L

G

G

P

V

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

L

F

S

K

M

M

L

L

C

T

T

G

I

D

L

T

N

T

P

V

T

T

S

S

G

G

T

D

A

A

T

T

V

V

N

A

G

G

Y

K

D

S

I

I

L

L

T

T

E

N

S

I

E

S

K

E

V

V

R

H

Q

Q

S

N

I

M

G

G

I

Y

V

C

F

P

Q

Q

S

F

I

D

S

A

I

I

D

D

D

E

R

L

L

L

T

T

G

G

R

R

E

E

N

H

L

L

K

Y

F

L

H

Y

A

A

M

R

L

L

Y

R

N

G

V

V

P

P

E

A

D

E

K

E

I

I

A

E

G

K

R

V

I

A

D

N

E

W

V

S

L

I

A

K

L

S

L

L

E

G

L

L

S

T

D

V

R

Y

A

A

D

D

D

C

L

L

V

A

R

G

T

T

Y

Y

S

S

G

G

M

N

I

K

R

R

R

K

L

L

E

S

M

T

A

A

R

I

G

A

L

L

L

I

H

G

H

C

P

P

H

P

I

L

L

V

F

L

L

L

D

D

E

E

P

P

T

T

I

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

E

R

H

M

I

L

W

W

S

N

Y

V

I

I

Q

V

E

S

L

I

T

I

R

R

K

E

K

G

K

R

G

A

D

-

L

-

T

-

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

D

E

E

E

A

C

D

E

L

A

L

L

C

C

D

T

E

R

V

L

A

A

I

I

-

T

-

I

-

Q

-

H

-

L

-

K

-

S

-

K

I

F

D

G

K

D

G

G

Q

Y

I

I

I

V

V

T

R

M

D

L

T

L

P

P

-

D

-

L

G

N

N

P

L

V

K

E

Q

Q

D

F

L

F

Q

Q

G

G

E

N

K

F

I

R

S

H

F

Y

N

N

M

M

Sites not aligning to the query:

7roqA Alternative structure of human abca1
31% identity, 95% coverage: 16:329/329 of query aligns to 784:1103/1831 of 7roqA

query
sites
7roqA

L

L

T

V

A

K

V

V

N

D

S

G

L

L

N

A

L

L

A

N

I

F

H

Y

K

E

G

G

T

Q

I

I

F

T

G

S

L

F

L

L

G

G

P

H

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

L

M

S

S

M

I

L

L

C

T

T

G

I

L

L

F

N

P

P

P

T

T

S

S

G

G

T

T

A

A

T

Y

V

I

N

L

G

G

Y

K

D

D

I

I

L

R

T

S

E

E

S

M

E

S

K

T

V

I

R

R

Q

Q

S

N

I

L

G

G

I

V

V

C

F

P

Q

Q

S

H

I

N

S

V

I

L

D

F

D

D

R

M

L

L

T

T

G

V

R

E

E

E

N

H

L

I

K

W

F

F

H

Y

A

A

M

R

L

L

Y

K

N

G

V

L

P

S

E

E

D

K

K

H

I

V

A

K

G

A

R

E

I

M

D

E

E

Q

V

M

L

A

A

L

L

D

L

V

E

G

L

L

S

S

D

S

R

K

A

L

D

K

D

S

L

K

V

T

R

S

T

Q

Y

L

S

S

G

G

M

M

I

Q

R

R

R

K

L

L

E

S

M

V

A

A

R

L

G

A

L

F

L

V

H

G

P

S

H

K

I

V

L

V

F

I

L

L

D

D

E

E

P

P

T

T

I

A

G

G

L

V

D

D

P

P

Q

Y

T

S

R

R

E

R

H

G

I

I

W

W

S

-

Y

-

I

-

Q

-

E

E

L

L

T

L

R

L

K

K

K

Y

K

R

G

Q

D

G

L

R

T

T

V

I

L

I

L

L

T

S

T

T

H

H

Y

H

M

M

D

D

E

E

A

A

D

D

L

V

L

L

C

G

D

D

E

R

V

I

A

A

I

I

D

S

K

H

G

G

Q

K

I

L

I

C

V

C

R

V

D

G

T

S

P

S

G

L

N

F

L

L

K

K

Q

N

D

Q

L

L

Q

G

G

T

E

G

K

Y

I

Y

S

L

F

T

N

L

M

V

K

K

S

K

P

L

E

T

I

I

L

D

A

V

S

S

L

A

L

I

S

S

D

N

N

L

P

I

R

R

I

-

C

-

K

K

V

H

S

V

T

S

E

E

S

A

G

R

L

L

L

V

E

E

-

D

-

I

-

G

-

H

-

E

-

L

-

T

-

Y

-

V

-

L

-

P

V

Y

Q

E

V

A

I

A

H

K

N

E

G

G

N

A

I

F

V

V

Q

E

E

L

I

F

F

H

D

E

I

I

-

D

A

D

R

R

E

L

A

S

G

D

I

L

E

G

I

I

D

S

H

S

I

Y

S

G

I

I

H

S

E

E

S

T

M

L

H

E

D

E

V

I

F

F

L

L

H

K

Y

V

T

A

G

W

K

K

E

L

I

T

R

Q

K

Q

E

Q

E

F

D

V

D

A

N

L

F

L

T

W

S

K

Q

R

Y

L

R

L

K

I

S

A

R

R

R

S

R

R

Sites not aligning to the query:

binding cholesterol: 688, 1106, 1426, 1526, 1527

Query Sequence

>WP_109942363.1 NCBI__GCF_003173335.1:WP_109942363.1
MVAIQTYNLTKQFGTLTAVNSLNLAIHKGTIFGLLGPNGAGKSTLLSMLCTILNPTSGTA
TVNGYDILTESEKVRQSIGIVFQSISIDDRLTGRENLKFHAMLYNVPEDKIAGRIDEVLA
LLELSDRADDLVRTYSGGMIRRLEMARGLLHHPHILFLDEPTIGLDPQTREHIWSYIQEL
TRKKKGDLTVLLTTHYMDEADLLCDEVAIIDKGQIIVRDTPGNLKQDLQGEKISFNMKSP
EILASLLSDNPRICKVSTESGLLEVQVIHNGNIVQEIFDIAREAGIEIDHISIHESSMHD
VFLHYTGKEIRKEEDDNFTSQYRKSRRRR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory