SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_109967319.1 NCBI__GCF_003173355.1:WP_109967319.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X1K9 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
46% identity, 99% coverage: 1:287/291 of query aligns to 1:288/294 of Q9X1K9

query
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Q9X1K9

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DID 166:168 (≠ DIN 167:169) mutation to AAA: Exists as a monomer in solution. Decreased activity and substrate affinity. Reduced thermal stability.

1o5kA Crystal structure of dihydrodipicolinate synthase (tm1521) from thermotoga maritima at 1.80 a resolution
46% identity, 99% coverage: 1:287/291 of query aligns to 2:289/295 of 1o5kA

query
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1o5kA

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active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K162 (= K162), I207 (= I205)
binding calcium ion: E65 (= E67), D68 (≠ G70)

Q07607 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; Protein MosA; EC 4.3.3.7 from Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
43% identity, 99% coverage: 1:289/291 of query aligns to 1:287/292 of Q07607

query
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Q07607

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K161 (= K162) active site, Schiff-base intermediate with substrate

5ktlA Dihydrodipicolinate synthase from the industrial and evolutionarily important cyanobacteria anabaena variabilis. (see paper)
42% identity, 100% coverage: 2:291/291 of query aligns to 5:293/295 of 5ktlA

query
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5ktlA

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active site: T47 (≠ S46), Y110 (= Y109), Y137 (= Y134), R142 (= R139), K165 (= K162), V207 (≠ I205)
binding manganese (ii) ion: S51 (= S50), L54 (= L53), E59 (≠ H58), E65 (≠ V64), Q68 (≠ E67)

2atsA Dihydrodipicolinate synthase co-crystallised with (s)-lysine
42% identity, 99% coverage: 1:289/291 of query aligns to 1:288/292 of 2atsA

query
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2atsA

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G

D

G

G

K

R

V

I

P

P

V

V

L

I

A

A

G

G

T

T

G

G

S

A

N
|
N

N

A

T

T

S

A

E
|
E

A

A

I

I

R

S

F

L

T

T

K

Q

A

R

A

F

K

N

D

D

A

S

G

G

A

I

D

V

G

G

V

C

L

L

V

T

I

V

S

T

P

P

Y
|
Y

N

N

K

R

P

P

N

S

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

F

T

K

A

A

L

I

A

A

D

E

-

H

L

T

D

D

I

L

P

P

V

Q

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

G

G

Q

C

N

D

L

L

A

L

P

P

E

E

L

T

I

V

A

G

E

R

L

L

A

A

N

K

H

V

P

K

N

N

I

I

V

I

G

G

V

I

K
|
K

E

E

A

A

S

T

G

G

D

N

I

L

N

T

Q

R

I

V

S

N

S

Q

I

I

I

K

E

E

L

L

T

V

L

S

D

D

N

-

D

D

F

F

S

V

V

L

I

L

S

S

G

G

D

D

G

A

M

S

T

A

L

L

P

D

I

F

L

M

S

Q

L

L

G

G

D

H

G

G

V

V

I
|
I

S

S

V

V

A

T

A

A

N

N

I

V

V

A

P

A

K

R

P

D

M

M

I

A

E

Q

M

M

Y

C

D

K

A

L

A

A

K

A

K

E

G

G

D

H

Y

F

E

A

T

E

A

A

R

R

S

V

I

I

H

N

Y

Q

K

R

L

L

A

M

P

P

L

L

I

H

K

N

A

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

C

E

K

M

E

R

L

G

G

M

L

-

V

A

A

A

T

G

D

P

T

V

L

R

R

L

L

P

P

L

M

D

T

E

P

A

I

S

T

Q

D

T

S

T

G

V

R

E

E

K

T

L

V

Q

R

E

A

V

A

L

L

T

K

H

H

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K161 (= K162), I203 (= I205)
binding d-lysine: A49 (= A51), L51 (= L53), H53 (≠ S55), H56 (= H58), N80 (= N82), E84 (= E86), Y106 (= Y108)

P0A6L2 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Escherichia coli (strain K12) (see 7 papers)
42% identity, 99% coverage: 1:289/291 of query aligns to 1:288/292 of P0A6L2

query
sites
P0A6L2

M

M

F

F

E

T

G

G

V

S

F

I

P

V

A

A

L

I

I

V

T

T

P

P

F

M

Q

D

R

E

N

K

A

G

G

-

K

-

D

N

L

V

D

C

V

R

D

A

G

S

L

L

R

K

S

K

N

L

I

I

T

D

F

Y

L

H

L

V

Q

A

S

S

G

G

V

T

H

S

G

A

L

I

V

V

P

S

C

V

G

G

S
x
T

T
|
T

G

G

E

E

S

S

A

A

T

T

L

L

S

N

S

H

A

D

E

E

H

H

E

A

K

D

V

V

E

M

V

M

T

T

L

L

E

D

E

L

A

A

G

D

G

G

K

R

V

I

P

P

V

V

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

A

T

T

S

A

E

E

A

A

I

I

R

S

F

L

T

T

K

Q

A

R

A

F

K

N

D

D

A

S

G

G

A

I

D

V

G

G

V

C

L

L

V

T

I

V

S

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

N

S

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

F

T

K

A

A

L

I

A

A

D

E

-

H

L

T

D

D

I

L

P

P

V

Q

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

G

G

Q

C

N

D

L

L

A

L

P

P

E

E

L

T

I

V

A

G

E

R

L

L

A

A

N

K

H

V

P

K

N

N

I

I

V

I

G

G

V

I

K
|
K

E

E

A

A

S

T

G

G

D

N

I

L

N

T

Q

R

I

V

S

N

S

Q

I

I

I

K

E

E

L

L

T

V

L

S

D

D

N

-

D

D

F

F

S

V

V

L

I

L

S

S

G

G

D

D

G

A

M

S

T

A

L

L

P

D

I

F

L

M

S

Q

L
|
L

G

G

D

H

G

G

V

V

I
|
I

S

S

V

V

A

T

A

A

N

N

I

V

V

A

P

A

K

R

P

D

M

M

I

A

E

Q

M

M

Y

C

D

K

A

L

A

A

K

A

K

E

G

G

D

H

Y

F

E

A

T

E

A

A

R

R

S

V

I

I

H

N

Y

Q

K

R

L

L

A

M

P

P

L

L

I

H

K

N

A

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

C

E

K

M

E

R

L

G

G

M

L

-

V

A

A

A

T

G

D

P

T

V

L

R

R

L

L

P

P

L

M

D

T

E

P

A

I

S

T

Q

D

T

S

T

G

V

R

E

E

K

T

L

V

Q

R

E

A

V

A

L

L

T

K

H

H

T44 (≠ S46) mutation to S: 8% of wild-type activity. 4-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.; mutation to V: Reduced kcat by 99.9%.
T45 (= T47) binding pyruvate
Y107 (= Y109) mutation to F: Reduced kcat by 90%.; mutation to W: Reduced activity by 95%. Reduced affinity for both substrates. Exists as a mixture of monomer, dimer and tetramer in solution. Has significantly lower thermal stability than the wild-type enzyme.
Y133 (= Y134) mutation to F: Reduced kcat by 99.7%. Reduced affinity for both substrates.
R138 (= R139) mutation R->A,H: Strongly increased KM for L-aspartate 4-semialdehyde. No effect on KM for pyruvate. Reduced activity by 99.7%.
K161 (= K162) mutation to A: 0.1% of wild-type activity. 3-fold decrease in affinity for pyruvate, and 2-fold decrease in that for (S)-ASA.; mutation to R: 0.35% of wild-type activity. 3-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.
L197 (= L199) mutation L->Y,D: 1.4 to 2.5% of wild-type activity. Decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA. Exists as a dimer in solution.
I203 (= I205) binding pyruvate

5t25A Kinetic, spectral and structural characterization of the slow binding inhibitor acetopyruvate with dihydrodipicolinate synthase from escherichia coli.
42% identity, 99% coverage: 1:289/291 of query aligns to 2:289/293 of 5t25A

query
sites
5t25A

M

M

F

F

E

T

G

G

V

S

F

I

P

V

A

A

L

I

I

V

T

T

P

P

F

M

Q

D

R

E

N

K

A

G

G

-

K

-

D

N

L

V

D

C

V

R

D

A

G

S

L

L

R

K

S

K

N

L

I

I

T

D

F

Y

L

H

L

V

Q

A

S

S

G

G

V

T

H

S

G

A

L

I

V

V

P

S

C

V

G

G

S
x
T

T

T

G

G

E

E

S

S

A
|
A

T

T

L
|
L

S

N

S
x
H

A

D

E

E

H

H

E

A

K

D

V

V

E

M

V

M

T

T

L

L

E

D

E

L

A

A

G

D

G

G

K

R

V

I

P

P

V

V

L

I

A

A

G

G

T

T

G

G

S

A

N
|
N

N

A

T

T

S

A

E
|
E

A

A

I

I

R

S

F

L

T

T

K

Q

A

R

A

F

K

N

D

D

A

S

G

G

A

I

D

V

G

G

V

C

L

L

V

T

I

V

S

T

P

P

Y
|
Y

N

N

K

R

P

P

N

S

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

F

T

K

A

A

L

I

A

A

D

E

-

H

L

T

D

D

I

L

P

P

V

Q

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

G

G

Q

C

N

D

L

L

A

L

P

P

E

E

L

T

I

V

A

G

E

R

L

L

A

A

N

K

H

V

P

K

N

N

I

I

V

I

G

G

V

I

K
|
K

E

E

A

A

S

T

G

G

D

N

I

L

N

T

Q

R

I

V

S

N

S

Q

I

I

I

K

E

E

L

L

T

V

L

S

D

D

N

-

D

D

F

F

S

V

V

L

I

L

S

S

G

G

D

D

G

A

M

S

T

A

L

L

P

D

I

F

L

M

S

Q

L

L

G

G

D

H

G

G

V

V

I
|
I

S

S

V

V

A

T

A

A

N

N

I

V

V

A

P

A

K

R

P

D

M

M

I

A

E

Q

M

M

Y

C

D

K

A

L

A

A

K

A

K

E

G

G

D

H

Y

F

E

A

T

E

A

A

R

R

S

V

I

I

H

N

Y

Q

K

R

L

L

A

M

P

P

L

L

I

H

K

N

A

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

C

E

K

M

E

R

L

G

G

M

L

-

V

A

A

A

T

G

D

P

T

V

L

R

R

L

L

P

P

L

M

D

T

E

P

A

I

S

T

Q

D

T

S

T

G

V

R

E

E

K

T

L

V

Q

R

E

A

V

A

L

L

T

K

H

H

active site: T45 (≠ S46), Y108 (= Y109), Y134 (= Y134), R139 (= R139), K162 (= K162), I204 (= I205)
binding lysine: A50 (= A51), L52 (= L53), H54 (≠ S55), N81 (= N82), E85 (= E86), Y107 (= Y108)

3i7sA Dihydrodipicolinate synthase mutant - k161a - with the substrate pyruvate bound in the active site. (see paper)
41% identity, 99% coverage: 1:289/291 of query aligns to 1:288/292 of 3i7sA

query
sites
3i7sA

M

M

F

F

E

T

G

G

V

S

F

I

P

V

A
|
A

L

I

I

V

T

T

P

P

F

M

Q

D

R

E

N

K

A

G

G

-

K

-

D

N

L

V

D

C

V

R

D

A

G

S

L

L

R

K

S

K

N

L

I

I

T

D

F

Y

L

H

L

V

Q

A

S

S

G

G

V

T

H

S

G

A

L

I

V

V

P

S

C

V

G

G

S
x
T

T
|
T

G

G

E

E

S

S

A

A

T

T

L

L

S

N

S

H

A

D

E

E

H

H

E

A

K

D

V

V

E

M

V

M

T

T

L

L

E

D

E

L

A

A

G

D

G

G

K

R

V

I

P

P

V

V

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

A

T

T

S

A

E

E

A

A

I

I

R

S

F

L

T

T

K

Q

A

R

A

F

K

N

D

D

A

S

G

G

A

I

D

V

G

G

V

C

L

L

V

T

I

V

S

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

N

S

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

F

T

K

A

A

L

I

A

A

D

E

-

H

L

T

D

D

I

L

P

P

V

Q

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

G

G

Q

C

N

D

L

L

A

L

P

P

E

E

L

T

I

V

A

G

E

R

L

L

A

A

N

K

H

V

P

K

N

N

I

I

V

I

G

G

V

I

K
x
R

E

E

A

A

S

T

G

G

D

N

I

L

N

T

Q

R

I

V

S

N

S

Q

I

I

I

K

E

E

L

L

T

V

L

S

D

D

N

-

D

D

F

F

S

V

V

L

I

L

S

S

G

G

D

D

G

A

M

S

T

A

L

L

P

D

I

F

L

M

S

Q

L

L

G

G

D

H

G

G

V

V

I
|
I

S

S

V

V

A

T

A

A

N

N

I

V

V

A

P

A

K

R

P

D

M

M

I

A

E

Q

M

M

Y

C

D

K

A

L

A

A

K

A

K

E

G

G

D

H

Y

F

E

A

T

E

A

A

R

R

S

V

I

I

H

N

Y

Q

K

R

L

L

A

M

P

P

L

L

I

H

K

N

A

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

C

E

K

M

E

R

L

G

G

M

L

-

V

A

A

A

T

G

D

P

T

V

L

R

R

L

L

P

P

L

M

D

T

E

P

A

I

S

T

Q

D

T

S

T

G

V

R

E

E

K

T

L

V

Q

R

E

A

V

A

L

L

T

K

H

H

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), R161 (≠ K162), I203 (= I205)
binding pyruvic acid: A8 (= A8), T44 (≠ S46), T45 (= T47), Y133 (= Y134)

4pfmA Shewanella benthica dhdps with lysine and pyruvate
45% identity, 95% coverage: 1:276/291 of query aligns to 2:276/295 of 4pfmA

query
sites
4pfmA

M

M

F

I

E

N

G

G

V

S

F

I

P

V

A

A

L

L

I

I

T

T

P

P

F

L

Q

-

R

-

N

N

A

S

G

D

K

G

D

T

L

V

D

D

V

Y

D

T

G

S

L

L

R

E

S

K

N

L

I

V

T

E

F

Y

L

H

L

I

Q

T

S

E

G

G

V

T

H

D

G

A

L

I

V

V

P

A

C

V

G

G

S
x
T

T

T

G

G

E

E

S
|
S

A
|
A

T

T

L
|
L

S

P

S

I

A

S

E

E

H
|
H

E

I

K

A

V

V

E

G

V

Q

T

T

L

V

E

K

E

F

A

A

G

S

G

G

K

R

V

I

P

P

V

V

L

I

A

G

G

G

T

N

G

G

S

A

N
|
N

N

A

T

T

S

A

E
|
E

A

A

I

I

R

E

F

L

T

T

K

K

A

A

A

Q

K

N

D

K

A

L

G

G

A

V

D

A

G

A

V

M

L

L

V

G

I

V

S

T

P

P

Y
|
Y

N

N

K

K

P

P

N

S

R

P

S

K

G

G

L

L

V

I

K

A

H

H

Y

Y

T

T

A

A

L

V

-

A

A

A

D

S

L

T

D

D

I

I

P

P

V

Q

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

G

A

Q

V

N

D

L

M

A

L

P

P

E

E

L

T

I

I

A

A

E

Q

L

L

A

V

N

E

H

V

P

P

N

N

I

I

V

I

G

G

V

V

K
|
K

E

D

A

A

S

T

G

G

D

D

I

V

N

A

Q

R

I

V

S

K

S

Q

I

L

I

R

E

D

L

L

T

C

L

-

D

G

N

N

D

D

F

F

S

L

V

L

I

Y

S

S

G

G

D

D

G

A

M

T

T

A

L

R

P

E

I

F

L

L

S

T

L

L

G

G

D

D

G

G

V

V

I
|
I

S

S

V

V

A

A

A

N

N

N

I

I

V

V

P

P

K

K

P

L

M

F

I

K

E

L

M

M

Y

C

D

D

A

A

K

L

K

A

G

G

D

D

Y

T

E

Q

T

A

A

A

R

M

S

A

I

A

H

E

Y

D

K

Q

L

I

A

K

P

G

L

L

I

F

K

S

A

A

L

L

F

F

V

C

E

E

T

A

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

A

E

H

M

K

R

M

G

G

M

L

-

I

A

S

A

Q

G

G

P

D

V

I

R

R

L

L

P

P

L

L

D

T

E

E

A

L

S

S

active site: T45 (≠ S46), Y108 (= Y109), Y134 (= Y134), R139 (= R139), K162 (= K162), I204 (= I205)
binding lysine: S49 (= S50), A50 (= A51), L52 (= L53), H57 (= H58), N81 (= N82), E85 (= E86), Y107 (= Y108)

4dxvA Crystal structure of dihydrodipicolinate synthase from acinetobacter baumannii complexed with mg and cl ions at 1.80 a resolution
40% identity, 98% coverage: 2:287/291 of query aligns to 2:285/291 of 4dxvA

query
sites
4dxvA

F

I

E

Q

G

G

V

S

F

I

P

V

A

A

L

I

I

V

T

T

P

P

F

M

Q

L

R

K

N

D

A

G

G

G

K

-

D

-

L

V

D

D

V

W

D

K

G

S

L

L

R

E

S

K

N

L

I

V

T

E

F

W

L

H

L

I

Q

E

S

Q

G

G

V

T

H

N

G

S

L

I

V

V

P

A

C

V

G

G

S
x
T

T

T

G

G

E

E

S

A

A

S

T

T

L

L

S

S

S

M

A

E

E

E

H

H

E

T

K

Q

V

V

V

I

E

K

V

E

T

I

L

I

E

R

E

V

A

A

G

N

G

K

K

R

V

I

P

P

V

I

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

S

T

T

S

R

E

E

A

A

I

I

R

E

F

L

T

T

K

K

A

A

K

K

D

D

A

L

G

G

A

A

D

D

G

A

V

A

L

L

V

L

I

V

S
x
T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

N

T

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

Y

T

K

A

A

L

I

A

A

D

E

-

A

L

V

D

E

I

L

P

P

V

L

V

I

I

L

Y
|
Y

N
|
N

I
x
V

P
|
P

G

G

R
|
R

T
|
T

G

G

Q

V

N

D

L

L

A

S

P

N

E

D

L

T

I

A

A

V

E

R

L

L

A

A

N

E

H

I

P

P

N

N

I

I

V

V

G

G

V

I

K
|
K

E

D

A

A

S

T

G

G

D

D

I

V

N

P

Q

R

I

G

S

K

S

A

I

L

I

I

E

D

L

-

T

A

L

L

D

N

N

G

D

K

F

M

S

A

V

V

I

Y

S

S

G

G

D

D

G

E

M

T

T

A

L

W

P

E

I

L

L

M

S

L

L

L

G

G

G

A

D

D

G

G

V

N

I
|
I

S

S

V

V

A

T

A

A

N

N

I

I

V

A

P

P

K

K

P

A

M

M

I

S

E

E

M

V

Y

C

D

A

A

V

A

A

K

I

K

A

G

K

D

D

Y

E

E

Q

T

Q

A

A

R

K

S

T

I

L

H

N

Y

N

K

K

L

I

A

A

P

N

L

L

I

H

K

N

A

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

L

E

H

M

E

R

M

G

G

M

L

A

I

A

D

G

T

P

G

V

I

R

R

L

L

P

P

L

L

D

T

E

P

A

L

S

A

Q

E

T

Q

T

Y

V

R

E

E

K

P

L

L

Q

R

E

N

V

A

L

L

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K161 (= K162), I203 (= I205)
binding magnesium ion: T104 (≠ S106), N134 (= N135), V135 (≠ I136), P136 (= P137), T139 (= T140)

3u8gA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with oxalic acid at 1.80 a resolution
40% identity, 98% coverage: 2:287/291 of query aligns to 2:285/291 of 3u8gA

query
sites
3u8gA

F

I

E

Q

G

G

V

S

F

I

P

V

A
|
A

L

I

I

V

T

T

P

P

F

M

Q

L

R

K

N

D

A

G

G

G

K

-

D

-

L

V

D

D

V

W

D

K

G

S

L

L

R

E

S

K

N

L

I

V

T

E

F

W

L

H

L

I

Q

E

S

Q

G

G

V

T

H

N

G

S

L

I

V

V

P

A

C

V

G
|
G

S
x
T

T
|
T

G

G

E

E

S

A

A

S

T

T

L

L

S

S

S

M

A

E

E

E

H

H

E

T

K

Q

V

V

V

I

E

K

V

E

T

I

L

I

E

R

E

V

A

A

G

N

G

K

K

R

V

I

P

P

V

I

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

S

T

T

S

R

E

E

A

A

I

I

R

E

F

L

T

T

K

K

A

A

K

K

D

D

A

L

G

G

A

A

D

D

G

A

V

A

L
|
L

V

L

I

V

S

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

N

T

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

Y

T

K

A

A

L

I

A

A

D

E

-

A

L

V

D

E

I

L

P

P

V

L

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

G

G

Q

V

N

D

L

L

A

S

P

N

E

D

L

T

I

A

A

V

E

R

L

L

A

A

N

E

H

I

P

P

N

N

I

I

V

V

G

G

V

I

K
|
K

E

D

A

A

S

T

G

G

D

D

I

V

N

P

Q

R

I

G

S

K

S

A

I

L

I

I

E

D

L

-

T

A

L

L

D

N

N

G

D

K

F

M

S

A

V

V

I

Y

S

S

G

G

D

D

G

E

M

T

T

A

L

W

P

E

I

L

L

M

S

L

L

L

G

G

G

A

D

D

G

G

V

N

I
|
I

S

S

V

V

A

T

A

A

N

N

I

I

V

A

P

P

K

K

P

A

M

M

I

S

E

E

M

V

Y

C

D

A

A

V

A

A

K

I

K

A

G

K

D

D

Y

E

E

Q

T

Q

A

A

R

K

S

T

I

L

H

N

Y

N

K

K

L

I

A

A

P

N

L

L

I

H

K

N

A

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

L

E

H

M

E

R

M

G

G

M

L

A

I

A

D

G

T

P

G

V

I

R

R

L

L

P

P

L

L

D

T

E

P

A

L

S

A

Q

E

T

Q

T

Y

V

R

E

E

K

P

L

L

Q

R

E

N

V

A

L

L

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K161 (= K162), I203 (= I205)
binding oxalate ion: A8 (= A8), G43 (= G45), T44 (≠ S46), T45 (= T47), L101 (= L103), Y133 (= Y134), K161 (= K162)

3tdfA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 2-ketobutanoic acid at 1.99 a resolution
40% identity, 98% coverage: 2:287/291 of query aligns to 2:285/291 of 3tdfA

query
sites
3tdfA

F

I

E

Q

G

G

V

S

F

I

P

V

A
|
A

L

I

I

V

T

T

P

P

F

M

Q

L

R

K

N

D

A

G

G

G

K

-

D

-

L

V

D

D

V

W

D

K

G

S

L

L

R

E

S

K

N

L

I

V

T

E

F

W

L

H

L

I

Q

E

S

Q

G

G

V

T

H

N

G

S

L

I

V

V

P

A

C

V

G

G

S
x
T

T
|
T

G

G

E

E

S

A

A

S

T

T

L

L

S

S

S

M

A

E

E

E

H

H

E

T

K

Q

V

V

V

I

E

K

V

E

T

I

L

I

E

R

E

V

A

A

G

N

G

K

K

R

V

I

P

P

V

I

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

S

T

T

S

R

E

E

A

A

I

I

R

E

F

L

T

T

K

K

A

A

K

K

D

D

A

L

G

G

A

A

D

D

G

A

V

A

L

L

V

L

I

V

S

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

N

T

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

Y

T

K

A

A

L

I

A

A

D

E

-

A

L

V

D

E

I

L

P

P

V

L

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

G

G

Q

V

N

D

L

L

A

S

P

N

E

D

L

T

I

A

A

V

E

R

L

L

A

A

N

E

H

I

P

P

N

N

I

I

V

V

G

G

V

I

K
|
K

E

D

A

A

S

T

G

G

D

D

I

V

N

P

Q

R

I

G

S

K

S

A

I

L

I

I

E

D

L

-

T

A

L

L

D

N

N

G

D

K

F

M

S

A

V

V

I

Y

S

S

G

G

D

D

G

E

M

T

T

A

L

W

P

E

I

L

L

M

S

L

L

L

G

G

G

A

D

D

G

G

V

N

I
|
I

S

S

V

V

A

T

A

A

N

N

I

I

V

A

P

P

K

K

P

A

M

M

I

S

E

E

M

V

Y

C

D

A

A

V

A

A

K

I

K

A

G

K

D

D

Y

E

E

Q

T

Q

A

A

R

K

S

T

I

L

H

N

Y

N

K

K

L

I

A

A

P

N

L

L

I

H

K

N

A

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

L

E

H

M

E

R

M

G

G

M

L

A

I

A

D

G

T

P

G

V

I

R

R

L

L

P

P

L

L

D

T

E

P

A

L

S

A

Q

E

T

Q

T

Y

V

R

E

E

K

P

L

L

Q

R

E

N

V

A

L

L

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K161 (= K162), I203 (= I205)
binding 2-ketobutyric acid: A8 (= A8), T44 (≠ S46), T45 (= T47), Y133 (= Y134), K161 (= K162), I203 (= I205)

3tceA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 5-hydroxylysine at 2.6 a resolution
40% identity, 98% coverage: 2:287/291 of query aligns to 2:285/291 of 3tceA

query
sites
3tceA

F

I

E

Q

G

G

V

S

F

I

P

V

A

A

L

I

I

V

T

T

P

P

F

M

Q

L

R

K

N

D

A

G

G

G

K

-

D

-

L

V

D

D

V

W

D

K

G

S

L

L

R

E

S

K

N

L

I

V

T

E

F

W

L

H

L

I

Q

E

S

Q

G

G

V

T

H

N

G

S

L

I

V

V

P

A

C

V

G

G

S
x
T

T

T

G

G

E

E

S

A

A
x
S

T

T

L
|
L

S

S

S

M

A

E

E

E

H
|
H

E

T

K

Q

V

V

V

I

E

K

V

E

T

I

L

I

E

R

E

V

A

A

G

N

G

K

K

R

V

I

P

P

V

I

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

S

T

T

S

R

E

E

A

A

I

I

R

E

F

L

T

T

K

K

A

A

K

K

D

D

A

L

G

G

A

A

D

D

G

A

V

A

L

L

V

L

I

V

S

T

P

P

Y
|
Y

N

N

K

K

P

P

N

T

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

Y

T

K

A

A

L

I

A

A

D

E

-

A

L

V

D

E

I

L

P

P

V

L

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

G

G

Q

V

N

D

L

L

A

S

P

N

E

D

L

T

I

A

A

V

E

R

L

L

A

A

N

E

H

I

P

P

N

N

I

I

V

V

G

G

V

I

K
|
K

E

D

A

A

S

T

G

G

D

D

I

V

N

P

Q

R

I

G

S

K

S

A

I

L

I

I

E

D

L

-

T

A

L

L

D

N

N

G

D

K

F

M

S

A

V

V

I

Y

S

S

G

G

D

D

G

E

M

T

T

A

L

W

P

E

I

L

L

M

S

L

L

L

G

G

G

A

D

D

G

G

V

N

I
|
I

S

S

V

V

A

T

A

A

N

N

I

I

V

A

P

P

K

K

P

A

M

M

I

S

E

E

M

V

Y

C

D

A

A

V

A

A

K

I

K

A

G

K

D

D

Y

E

E

Q

T

Q

A

A

R

K

S

T

I

L

H

N

Y

N

K

K

L

I

A

A

P

N

L

L

I

H

K

N

A

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

L

E

H

M

E

R

M

G

G

M

L

A

I

A

D

G

T

P

G

V

I

R

R

L

L

P

P

L

L

D

T

E

P

A

L

S

A

Q

E

T

Q

T

Y

V

R

E

E

K

P

L

L

Q

R

E

N

V

A

L

L

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K161 (= K162), I203 (= I205)
binding 5-hydroxylysine: S49 (≠ A51), L51 (= L53), H56 (= H58), Y106 (= Y108)

3rk8A Crystal structure of the chloride inhibited dihydrodipicolinate synthase from acinetobacter baumannii complexed with pyruvate at 1.8 a resolution
40% identity, 98% coverage: 2:287/291 of query aligns to 2:285/291 of 3rk8A

query
sites
3rk8A

F

I

E

Q

G

G

V

S

F

I

P

V

A

A

L

I

I

V

T

T

P

P

F

M

Q

L

R

K

N

D

A

G

G

G

K

-

D

-

L

V

D

D

V

W

D

K

G

S

L

L

R

E

S

K

N

L

I

V

T

E

F

W

L

H

L

I

Q

E

S

Q

G

G

V

T

H

N

G

S

L

I

V

V

P

A

C

V

G

G

S
x
T

T

T

G

G

E

E

S

A

A

S

T

T

L

L

S

S

S

M

A

E

E

E

H

H

E

T

K

Q

V

V

V

I

E

K

V

E

T

I

L

I

E

R

E

V

A

A

G

N

G

K

K

R

V

I

P

P

V

I

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

S

T

T

S

R

E

E

A

A

I

I

R

E

F

L

T

T

K

K

A

A

K

K

D

D

A

L

G

G

A

A

D

D

G

A

V

A

L

L

V

L

I

V

S

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

N

T

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

Y

T

K

A

A

L

I

A

A

D

E

-

A

L

V

D

E

I

L

P

P

V

L

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

G

G

Q

V

N

D

L

L

A

S

P

N

E

D

L

T

I

A

A

V

E

R

L

L

A

A

N

E

H

I

P

P

N

N

I

I

V

V

G

G

V

I

K
|
K

E

D

A

A

S

T

G

G

D

D

I

V

N

P

Q

R

I

G

S

K

S

A

I

L

I

I

E

D

L

-

T

A

L

L

D

N

N

G

D

K

F

M

S

A

V

V

I

Y

S

S

G
|
G

D

D

G

E

M

T

T

A

L

W

P

E

I

L

L

M

S

L

L

L

G

G

G

A

D

D

G

G

V

N

I
|
I

S

S

V

V

A

T

A

A

N

N

I

I

V

A

P

P

K

K

P

A

M

M

I

S

E

E

M

V

Y

C

D

A

A

V

A

A

K

I

K

A

G

K

D

D

Y

E

E

Q

T

Q

A

A

R

K

S

T

I

L

H

N

Y

N

K

K

L

I

A

A

P

N

L

L

I

H

K

N

A

I

L

L

F
|
F

V

C

E

E

T

S

N

N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

L

E

H

M

E

R

M

G

G

M

L

A

I

A

D

G

T

P

G

V

I

R

R

L

L

P

P

L

L

D

T

E

P

A

L

S

A

Q

E

T

Q

T

Y

V

R

E

E

K

P

L

L

Q

R

E

N

V

A

L

L

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K161 (= K162), I203 (= I205)
binding pyruvic acid: R138 (= R139), K161 (= K162), G186 (= G188), F244 (= F246)

3pueB Crystal structure of the complex of dhydrodipicolinate synthase from acinetobacter baumannii with lysine at 2.6a resolution
40% identity, 98% coverage: 2:287/291 of query aligns to 2:285/291 of 3pueB

query
sites
3pueB

F

I

E

Q

G

G

V

S

F

I

P

V

A

A

L

I

I

V

T

T

P

P

F

M

Q

L

R

K

N

D

A

G

G

G

K

-

D

-

L

V

D

D

V

W

D

K

G

S

L

L

R

E

S

K

N

L

I

V

T

E

F

W

L

H

L

I

Q

E

S

Q

G

G

V

T

H

N

G

S

L

I

V

V

P

A

C

V

G

G

S
x
T

T

T

G

G

E

E

S

A

A

S

T

T

L

L

S

S

S

M

A

E

E

E

H

H

E

T

K

Q

V

V

V

I

E

K

V

E

T

I

L

I

E

R

E

V

A

A

G

N

G

K

K

R

V

I

P

P

V

I

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

S

T

T

S

R

E

E

A

A

I

I

R

E

F

L

T

T

K

K

A

A

K

K

D

D

A

L

G

G

A

A

D

D

G

A

V

A

L

L

V

L

I

V

S

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

N

T

R

Q

S

E

G

G

L

L

V

Y

K

Q

H

H

Y

Y

T

K

A

A

L

I

A

A

D

E

-

A

L

V

D

E

I

L

P

P

V

L

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

G

G

Q

V

N

D

L

L

A

S

P

N

E

D

L

T

I

A

A

V

E

R

L

L

A

A

N

E

H

I

P

P

N

N

I

I

V

V

G

G

V

I

K
|
K

E

D

A

A

S

T

G

G

D

D

I

V

N

P

Q

R

I

G

S

K

S

A

I

L

I

I

E

D

L

-

T

A

L

L

D

N

N

G

D

K

F

M

S

A

V

V

I

Y

S

S

G

G

D

D

G

E

M

T

T

A

L

W

P

E

I

L

L

M

S

L

L

L

G

G

G

A

D

D

G

G

V

N

I
|
I

S

S

V

V

A

T

A

A

N

N

I

I

V

A

P

P

K

K

P

A

M

M

I

S

E

E

M

V

Y

C

D

A

A

V

A

A

K

I

K

A

G

K

D

D

Y

E

E

Q

T

Q

A

A

R

K

S

T

I

L

H

N

Y

N

K

K

L

I

A

A

P

N

L

L

I

H

K

N

A

I

L

L

F
|
F

V

C

E

E

T

S

N
|
N

P

P

V

I

P

P

I

V

K

K

K

W

A

A

T

L

E

H

M

E

R

M

G

G

M

L

A

I

A

D

G

T

P

G

V

I

R

R

L

L

P

P

L

L

D

T

E

P

A

L

S

A

Q

E

T

Q

T

Y

V

R

E

E

K

P

L

L

Q

R

E

N

V

A

L

L

active site: T44 (≠ S46), Y107 (= Y109), Y133 (= Y134), R138 (= R139), K161 (= K162), I203 (= I205)
binding lysine: R138 (= R139), F244 (= F246), N248 (= N250)

3s8hA Structure of dihydrodipicolinate synthase complexed with 3- hydroxypropanoic acid(hpa)at 2.70 a resolution
43% identity, 99% coverage: 1:287/291 of query aligns to 1:285/292 of 3s8hA

query
sites
3s8hA

M

M

F

I

E

A

G

G

V

S

F

M

P

V

A
|
A

L
|
L

I

V

T

T

P

P

F

F

Q

-

R

-

N

D

A

A

G

Q

K

G

D

R

L

L

D

D

V

W

D

D

G

S

L

L

R

A

S

K

N

L

I

V

T

D

F

F

L

H

L

L

Q

Q

S

D

G

G

V

T

H

N

G

A

L

I

V
|
V

P

A

C

V

G
|
G

S
x
T

T
|
T

G
|
G

E

E

S

S

A

A

T

T

L

L

S

D

S

V

A

E

E

E

H

H

E

I

K

Q

V

V

E

R

V

R

T

V

L

V

E

D

E

Q

A

V

G

K

G

G

K

R

V

I

P

P

V

V

L

I

A

A

G

G

T

T

G

G

S

A

N

N

N

S

T

T

S

R

E

E

A

A

I

V

R

A

F

L

T

T

K

E

A

A

K

K

D

S

A

G

G

G

A

A

D

D

G

A

V

C

L

L

V

L

I

V

S

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

N

T

R

Q

S

E

G

G

L

M

V

Y

K

Q

H

H

Y

F

T

R

A

H

L

I

A

A

D

E

-

A

L

V

D

A

I

I

P

P

V

Q

V

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

G

S

Q

C

N

D

L

M

A

L

P

P

E

E

L

T

I

V

A

E

E

R

L

L

A

S

N

K

H

V

P

P

N

N

I

I

V

I

G

G

V

I

K
|
K

E

E