SitesBLAST
Comparing WP_109967711.1 NCBI__GCF_003173355.1:WP_109967711.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
44% identity, 94% coverage: 15:392/401 of query aligns to 16:390/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: I18 (= I17), R19 (= R18), G35 (= G34), G36 (= G35), G95 (= G100), S96 (= S101), Q97 (= Q102), Y121 (= Y126), N170 (= N175), D198 (= D203), Y201 (= Y206), S231 (= S232), S233 (= S234), K234 (= K235), R241 (= R242), R364 (= R366)
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
44% identity, 94% coverage: 15:392/401 of query aligns to 16:390/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G100), S96 (= S101), Q97 (= Q102), Y121 (= Y126), N170 (= N175), D198 (= D203), A200 (= A205), Y201 (= Y206), S231 (= S232), S233 (= S234), K234 (= K235), R241 (= R242)
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
44% identity, 94% coverage: 15:392/401 of query aligns to 20:394/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I22 (= I17), R23 (= R18), G39 (= G34), G40 (= G35), G99 (= G100), S100 (= S101), Q101 (= Q102), Y125 (= Y126), N174 (= N175), D202 (= D203), Y205 (= Y206), S235 (= S232), S237 (= S234), K238 (= K235), R245 (= R242), R368 (= R366)
Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
44% identity, 94% coverage: 15:392/401 of query aligns to 20:394/397 of Q72LL6
- S20 (= S15) mutation to E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
- R23 (= R18) mutation to A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; mutation to Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
- G40 (= G35) binding substrate
- Y70 (= Y65) binding pyridoxal 5'-phosphate
- N174 (= N175) binding pyridoxal 5'-phosphate; binding substrate
- R245 (= R242) binding pyridoxal 5'-phosphate
- R368 (= R366) binding substrate
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
43% identity, 94% coverage: 15:392/401 of query aligns to 17:386/389 of 2z1yA
- binding leucine: G32 (= G35), Y117 (= Y126), R360 (= R366)
- binding pyridoxal-5'-phosphate: G91 (= G100), S92 (= S101), Q93 (= Q102), Y117 (= Y126), N166 (= N175), D194 (= D203), Y197 (= Y206), S227 (= S232), S229 (= S234), K230 (= K235), R237 (= R242)
3wx9A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, gla, 4ad, 2og, glu and kya
42% identity, 97% coverage: 5:393/401 of query aligns to 10:403/404 of 3wx9A
- binding 4-(2-aminophenyl)-2,4-dioxobutanoic acid: R23 (= R18), G40 (= G35), L41 (= L36)
- binding 2-oxoglutaric acid: D213 (= D204), P214 (≠ A205), Y215 (= Y206), G216 (= G207), E217 (= E208), G241 (= G231), T242 (≠ S232), I246 (= I236)
- binding (2E)-pent-2-enedioic acid: G40 (= G35), Y130 (= Y126), N184 (= N175), R376 (= R366)
- binding glutamic acid: S20 (= S15), V22 (≠ I17), L131 (= L127), V360 (≠ L350), A364 (≠ P354), R369 (≠ G359)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (= Y206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
3av7A Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with pmp, kyn as substrates and kya as products
42% identity, 97% coverage: 5:393/401 of query aligns to 10:403/404 of 3av7A
- binding 4-hydroxyquinoline-2-carboxylic acid: S20 (= S15), V22 (≠ I17), R23 (= R18), L131 (= L127), Q135 (= Q131), A364 (≠ P354), R369 (≠ G359)
- binding (2S)-2-amino-4-(2-aminophenyl)-4-oxobutanoic acid: R23 (= R18), G40 (= G35), Y130 (= Y126), L131 (= L127), A132 (≠ G128), N184 (= N175), R376 (= R366)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), V179 (= V170), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (= Y206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
3aowC Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with akg
42% identity, 97% coverage: 5:393/401 of query aligns to 10:403/404 of 3aowC
- binding 2-oxoglutaric acid: R23 (= R18), Y70 (= Y65), Y130 (= Y126), L275 (= L265)
- binding pyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), V179 (= V170), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (= Y206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
3aovA Crystal structure of pyrococcus horikoshii kynurenine aminotransferase in complex with plp
42% identity, 97% coverage: 5:393/401 of query aligns to 10:403/404 of 3aovA
- binding pyridoxal-5'-phosphate: G104 (= G100), S105 (= S101), Q106 (= Q102), Y130 (= Y126), V179 (= V170), N184 (= N175), D212 (= D203), P214 (≠ A205), Y215 (= Y206), T242 (≠ S232), S244 (= S234), K245 (= K235), R252 (= R242)
1wstA Crystal structure of multiple substrate aminotransferase (msat) from thermococcus profundus
40% identity, 95% coverage: 15:393/401 of query aligns to 17:401/403 of 1wstA
- binding pyridoxal-5'-phosphate: G102 (= G100), S103 (= S101), Q104 (= Q102), Y128 (= Y126), V177 (= V170), N182 (= N175), D210 (= D203), P212 (≠ A205), Y213 (= Y206), T240 (≠ S232), S242 (= S234), K243 (= K235), R250 (= R242)
1vp4A Crystal structure of a putative aminotransferase (tm1131) from thermotoga maritima msb8 at 1.82 a resolution
40% identity, 96% coverage: 10:394/401 of query aligns to 21:416/420 of 1vp4A
- binding pyridoxal-5'-phosphate: G112 (= G100), S113 (= S101), Q114 (= Q102), Y138 (= Y126), N194 (= N175), D222 (= D203), P224 (≠ A205), Y225 (= Y206), T252 (≠ S232), S254 (= S234), K255 (= K235), R262 (= R242)
2zc0A Crystal structure of an archaeal alanine:glyoxylate aminotransferase (see paper)
33% identity, 91% coverage: 28:390/401 of query aligns to 35:403/405 of 2zc0A