SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_109967727.1 NCBI__GCF_003173355.1:WP_109967727.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
73% identity, 100% coverage: 1:288/288 of query aligns to 2:289/290 of 5mr0D

query
sites
5mr0D

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active site: F32 (= F31), G34 (= G33), K150 (= K149), E183 (= E182), L206 (= L205)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R50), G100 (= G99), L101 (= L100), K150 (= K149), Y154 (= Y153), E183 (= E182), G186 (= G185), D187 (= D186), L206 (= L205), I209 (= I208), T210 (= T209), G245 (= G244), T246 (= T245)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
71% identity, 100% coverage: 1:288/288 of query aligns to 3:290/290 of 5e25A

query
sites
5e25A

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active site: F33 (= F31), G35 (= G33), K151 (= K149), E184 (= E182), L207 (= L205)
binding 2-oxoglutaric acid: Y88 (= Y86), K151 (= K149), T247 (= T245), A248 (= A246)
binding pyridoxal-5'-phosphate: R52 (= R50), K151 (= K149), Y155 (= Y153), E184 (= E182), G187 (= G185), D188 (= D186), L207 (= L205), G209 (= G207), I210 (= I208), T211 (= T209), G246 (= G244), T247 (= T245)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
43% identity, 95% coverage: 3:276/288 of query aligns to 7:286/305 of 2ej0B

query
sites
2ej0B

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active site: F35 (= F31), G37 (= G33), K158 (= K149), E192 (= E182), L215 (= L205)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R50), Y163 (= Y153), E192 (= E182), G195 (= G185), E196 (≠ D186), L215 (= L205), G217 (= G207), I218 (= I208), T219 (= T209), G254 (= G244), T255 (= T245)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
42% identity, 95% coverage: 3:276/288 of query aligns to 7:278/297 of 2ej3A

query
sites
2ej3A

L

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active site: F35 (= F31), G37 (= G33), K150 (= K149), E184 (= E182), L207 (= L205)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G185), G246 (= G244), T247 (= T245), A248 (= A246)
binding pyridoxal-5'-phosphate: R58 (= R50), K150 (= K149), Y155 (= Y153), E184 (= E182), G187 (= G185), L207 (= L205), G209 (= G207), I210 (= I208), T211 (= T209), G246 (= G244), T247 (= T245)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
42% identity, 95% coverage: 3:276/288 of query aligns to 7:278/297 of 2eiyA

query
sites
2eiyA

L

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K

E

E

H

H

L

V

D

K

R
|
R

L

F

Y

Y

D

N

S

S

A

A

K

K

T

V

I

L

D

R

L

M

Q

E

I

I

P

P

L

F

S

A

K

P

E

E

M

L

T

E

E

E

A

A

I

I

L

K

E

E

T

V

C

V

R

R

K

R

N

N

N

G

L

Y

K

R

D

S

A

C

Y
|
Y

I

I

R

R

P

P

L

L

V

A

S

W

R

M

G

G

V

A

G

K

D

A

L

L

G

G

L

V

D

N

P

P

R

L

K

P

C

N

E

N

K

P

P

A

T

E

V

V

V

M

V

V

F

A

A

A

V

W

E

E

W

W

G

-

A

-

M

-

Y

-

G

-

D

-

L

-

Y

V

E

R

K

K

G

G

L

A

K

R

A

L

I

I

S

T

V

S

S

S

I

W

R

A

R

R

N

F

P

P

A

A

E

N

S

V

L

M

P

P

P

G

N

K

V

A

K
|
K

-

V

S

G

L

G

N

N

Y
|
Y

L

V

N

N

N

S

I

A

L

L

A

A

K

K

I

M

E

E

A

A

N

V

Y

A

K

A

G

G

G

A

D

D

E

E

A

A

I

L

F

L

D

D

T

E

N

E

G

G

Y

Y

V

V

S

A

E
|
E

G

G

S

S

G
|
G

D
x
E

N

N

I

L

F

F

V

F

V

V

K

R

N

D

G

G

S

V

I

I

S

Y

T

A

P

L

P

E

T

H

I

S

N

V

N

N

L
|
L

R

E

G
|
G

I
|
I

T
|
T

R

R

L

D

V

S

G

V

I

I

E

R

I

I

A

A

N

K

E

D

L

L

G

G

I

Y

N

E

I

V

S

Q

E

V

R

V

N

R

M

A

G

T

Y

R

Y

D

D

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

V

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

A

T

P

P

I

V

T

S

L

M

I

I

D

D

G

W

R

R

V

P

I

I

G

G

N

K

G

G

K

T

P

A

G

G

A

P

I

V

T

A

R

L

Q

R

M

L

M

R

E

E

K

V

F

Y

active site: F35 (= F31), G37 (= G33), K150 (= K149), E184 (= E182), L207 (= L205)
binding 4-methyl valeric acid: F35 (= F31), Y94 (= Y86), T247 (= T245), A248 (= A246)
binding pyridoxal-5'-phosphate: R58 (= R50), K150 (= K149), Y155 (= Y153), E184 (= E182), G187 (= G185), E188 (≠ D186), L207 (= L205), G209 (= G207), I210 (= I208), T211 (= T209), G246 (= G244), T247 (= T245)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
42% identity, 95% coverage: 3:276/288 of query aligns to 7:278/297 of 1wrvA

query
sites
1wrvA

L

I

Y

W

I

M

D

N

G

G

E

A

F

F

Y

V

S

P

R

Q

E

E

D

E

A

A

K

K

I

T

S

S

P

V

F

L

D

S

H

H

G

A

F

L

L

H

Y

Y

G

G

D

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

S

E

G

T

R

A

-

K

-

G

-

P

-

A

V

I

F

F

R

R

L

L

S

K

E

E

H

H

L

V

D

K

R
|
R

L

F

Y

Y

D

N

S

S

A

A

K

K

T

V

I

L

D

R

L

M

Q

E

I

I

P

P

L

F

S

A

K

P

E

E

M

L

T

E

E

E

A

A

I

I

L

K

E

E

T

V

C

V

R

R

K

R

N

N

N

G

L

Y

K

R

D

S

A

C

Y

Y

I

I

R

R

P

P

L

L

V

A

S

W

R

M

G

G

V

A

G

K

D

A

L

L

G

G

L

V

D

N

P

P

R

L

K

P

C

N

E

N

K

P

P

A

T

E

V

V

V

M

V

V

F

A

A

A

V

W

E

E

W

W

G

-

A

-

M

-

Y

-

G

-

D

-

L

-

Y

V

E

R

K

K

G

G

L

A

K

R

A

L

I

I

S

T

V

S

S

S

I

W

R

A

R

R

N

F

P

P

A

A

E

N

S

V

L

M

P

P

P

G

N

K

V

A

K
|
K

-

V

S

G

L

G

N

N

Y
|
Y

L

V

N

N

N

S

I

A

L

L

A

A

K

K

I

M

E

E

A

A

N

V

Y

A

K

A

G

G

G

A

D

D

E

E

A

A

I

L

F

L

D

D

T

E

N

E

G

G

Y

Y

V

V

S

A

E
|
E

G

G

S

S

G
|
G

D

E

N

N

I

L

F

F

V

F

V

V

K

R

N

D

G

G

S

V

I

I

S

Y

T

A

P

L

P

E

T

H

I

S

N

V

N

N

L
|
L

R

E

G
|
G

I
|
I

T
|
T

R

R

L

D

V

S

G

V

I

I

E

R

I

I

A

A

N

K

E

D

L

L

G

G

I

Y

N

E

I

V

S

Q

E

V

R

V

N

R

M

A

G

T

Y

R

Y

D

D

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

V

M

T

T

G

G

T
|
T

A

A

E

E

I

V

A

T

P

P

I

V

T

S

L

M

I

I

D

D

G

W

R

R

V

P

I

I

G

G

N

K

G

G

K

T

P

A

G

G

A

P

I

V

T

A

R

L

Q

R

M

L

M

R

E

E

K

V

F

Y

active site: F35 (= F31), G37 (= G33), K150 (= K149), E184 (= E182), L207 (= L205)
binding pyridoxal-5'-phosphate: R58 (= R50), K150 (= K149), Y155 (= Y153), E184 (= E182), G187 (= G185), L207 (= L205), G209 (= G207), I210 (= I208), T211 (= T209), T247 (= T245)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
42% identity, 95% coverage: 3:276/288 of query aligns to 7:275/294 of 2ej2A

query
sites
2ej2A

L

I

Y

W

I

M

D

N

G

G

E

A

F

F

Y

V

S

P

R

Q

E

E

D

E

A

A

K

K

I

T

S

S

P

V

F

L

D

S

H

H

G

A

F

L

L

H

Y

Y

G

G

D

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

S

E

G

T

R

A

-

K

-

G

-

P

-

A

V

I

F

F

R

R

L

L

S

K

E

E

H

H

L

V

D

K

R
|
R

L

F

Y

Y

D

N

S

S

A

A

K

K

T

V

I

L

D

R

L

M

Q

E

I

I

P

P

L

F

S

A

K

P

E

E

M

L

T

E

E

E

A

A

I

I

L

K

E

E

T

V

C

V

R

R

K

R

N

N

N

G

L

Y

K

R

D

S

A

C

Y
|
Y

I

I

R

R

P

P

L

L

V

A

S

W

R

M

G

G

V

A

G

K

D

A

L

L

G

G

L

V

D

N

P

P

R

L

K

P

C

N

E

N

K

P

P

A

T

E

V

V

V

M

V

V

F

A

A

A

V

W

E

E

W

W

G

G

A

A

M

-

Y

-

G

-

D

-

L

-

Y

-

E

-

K

-

G

-

L

-

K

R

A

L

I

I

S

T

V

S

S

S

I

W

R

A

R

R

N

F

P

P

A

A

E

N

S

V

L

M

P

P

P

G

N

K

V

A

K
|
K

-

V

S

G

L

G

N

N

Y
|
Y

L

V

N

N

N

S

I

A

L

L

A

A

K

K

I

M

E

E

A

A

N

V

Y

A

K

A

G

G

G

A

D

D

E

E

A

A

I

L

F

L

D

D

T

E

N

E

G

G

Y

Y

V

V

S

A

E
|
E

G

G

S

S

G
|
G

D
x
E

N

N

I

L

F

F

V

F

V

V

K

R

N

D

G

G

S

V

I

I

S

Y

T

A

P

L

P

E

T

H

I

S

N

V

N

N

L
|
L

R

E

G
|
G

I
|
I

T
|
T

R

R

L

D

V

S

G

V

I

I

E

R

I

I

A

A

N

K

E

D

L

L

G

G

I

Y

N

E

I

V

S

Q

E

V

R

V

N

R

M

A

G

T

Y

R

Y

D

D

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

V

M

T

T

G

G

T
|
T

A
|
A

A

A

E

E

I

V

A

T

P

P

I

V

T

S

L

M

I

I

D

D

G

W

R

R

V

P

I

I

G

G

N

K

G

G

K

T

P

A

G

G

A

P

I

V

T

A

R

L

Q

R

M

L

M

R

E

E

K

V

F

Y

active site: F35 (= F31), G37 (= G33), K147 (= K149), E181 (= E182), L204 (= L205)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R50), Y94 (= Y86), Y152 (= Y153), E181 (= E182), G184 (= G185), E185 (≠ D186), L204 (= L205), G206 (= G207), I207 (= I208), T208 (= T209), T244 (= T245), A245 (= A246)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
42% identity, 95% coverage: 3:276/288 of query aligns to 5:285/304 of 1iyeA

query
sites
1iyeA

L

I

Y

W

I

F

D

N

G

G

E

E

F

M

Y

V

S

R

R

W

E

E

D

D

A

A

K

K

I

V

S

H

P

V

F

M

D

S

H

H

G

A

F

L

L

H

Y

Y

G

G

D

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

C

Y

Y

S

D

G

S

R

H

-

K

-

G

-

P

-

V

V

V

F

F

R

R

L

H

S

R

E

E

H

H

L

M

D

Q

R
|
R

L

L

Y

H

D

D

S

S

A

A

K

K

T

I

I

Y

D

R

L

F

Q

P

I

V

P

S

L

Q

S

S

K

I

E

D

E

E

M

L

T

M

E

E

A

A

I

C

L

R

E

D

T

V

C

I

R

R

K

K

N

N

L

L

K

T

D

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

V

I

S

F

R

V

G

G

V

D

G

V

D

G

L

M

G

G

L

V

D

N

P

P

R

P

K

A

C

G

E

Y

K

S

P

T

T

D

V

V

V

I

F

A

A

A

V

F

E

P

W

W

G

G

A

A

M
x
Y

Y

L

G

G

-

A

D

E

L

A

Y

L

E

E

K

Q

G

G

L

I

K

D

A

A

I

M

S

V

V

S

S

S

I

W

R

N

R

R

N

A

P

A

A

P

E

N

S

T

L

I

P

P

P

T

N

A

V

A

K
|
K

S
x
A

L

G

-

G

N

N

Y
|
Y

L

L

N

S

I

L

L

L

A

V

K

G

I

S

E

E

A

A

N

R

Y

R

K

H

G

G

G

Y

D

Q

E

E

A

G

I

I

F

A

F

L

D

D

T

V

N

N

G

G

Y

Y

V

I

S

S

E
|
E

G

G

S

A

G
|
G

D
x
E

N
|
N

I

L

F

F

V

E

V

V

K

K

N

D

G

G

S

V

I

L

S

F

T

T

P

P

-

F

T

T

I

S

N

S

N

A

L
|
L

R

P

G
|
G

I
|
I

T
|
T

R

R

L

D

V

A

G

I

I

I

E

K

I

L

A

A

N

K

E

E

L

L

G

G

I

I

N

E

I

V

S

R

E

E

R

Q

N

V

M

L

G

S

Y

R

Y

E

D

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

V

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

A

T

P

P

I

V

T

R

L

S

I

V

D

D

G

G

R

I

V

Q

I

V

G

G

N

E

G

G

K

R

P

C

G

G

A

P

I

V

T

T

R

K

Q

R

M

I

M

Q

E

Q

K

A

F

F

active site: F33 (= F31), G35 (= G33), K156 (= K149), A157 (≠ S150), E190 (= E182), L214 (= L205)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R50), Y92 (= Y86), Y126 (≠ M120), K156 (= K149), Y161 (= Y153), E190 (= E182), G193 (= G185), E194 (≠ D186), N195 (= N187), L214 (= L205), G216 (= G207), I217 (= I208), T218 (= T209), G253 (= G244), T254 (= T245), A255 (= A246)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
42% identity, 95% coverage: 3:276/288 of query aligns to 5:285/304 of 1iydA

query
sites
1iydA

L

I

Y

W

I

F

D

N

G

G

E

E

F

M

Y

V

S

R

R

W

E

E

D

D

A

A

K

K

I

V

S

H

P

V

F

M

D

S

H

H

G

A

F

L

L

H

Y

Y

G

G

D

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

C

Y

Y

S

D

G

S

R

H

-

K

-

G

-

P

-

V

V

V

F

F

R

R

L

H

S

R

E

E

H

H

L

M

D

Q

R
|
R

L

L

Y

H

D

D

S

S

A

A

K

K

T

I

I

Y

D

R

L

F

Q

P

I

V

P

S

L

Q

S

S

K

I

E

D

E

E

M

L

T

M

E

E

A

A

I

C

L

R

E

D

T

V

C

I

R

R

K

K

N

N

L

L

K

T

D

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

V

I

S

F

R

V

G

G

V

D

G

V

D

G

L

M

G

G

L

V

D

N

P

P

R

P

K

A

C

G

E

Y

K

S

P

T

T

D

V

V

V

I

F

A

A

A

V

F

E

P

W

W

G

G

A

A

M
x
Y

Y

L

G

G

-

A

D

E

L

A

Y

L

E

E

K

Q

G

G

L

I

K

D

A

A

I

M

S

V

V

S

S

S

I

W

R

N

R

R

N

A

P

A

A

P

E

N

S

T

L

I

P

P

P

T

N

A

V

A

K
|
K

S
x
A

L

G

-

G

N

N

Y
|
Y

L

L

N

S

I

L

L

L

A

V

K

G

I

S

E

E

A

A

N

R

Y

R

K

H

G

G

G

Y

D

Q

E

E

A

G

I

I

F

A

F

L

D

D

T

V

N

N

G

G

Y

Y

V

I

S

S

E
|
E

G

G

S

A

G
|
G

D
x
E

N

N

I

L

F

F

V

E

V

V

K

K

N

D

G

G

S

V

I

L

S

F

T

T

P

P

-

F

T

T

I

S

N

S

N

A

L
|
L

R

P

G
|
G

I
|
I

T
|
T

R

R

L

D

V

A

G

I

I

I

E

K

I

L

A

A

N

K

E

E

L

L

G

G

I

I

N

E

I

V

S

R

E

E

R

Q

N

V

M

L

G

S

Y

R

Y

E

D

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

V

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

I

I

A

T

P

P

I

V

T

R

L

S

I

V

D

D

G

G

R

I

V

Q

I

V

G

G

N

E

G

G

K

R

P

C

G

G

A

P

I

V

T

T

R

K

Q

R

M

I

M

Q

E

Q

K

A

F

F

active site: F33 (= F31), G35 (= G33), K156 (= K149), A157 (≠ S150), E190 (= E182), L214 (= L205)
binding glutaric acid: Y92 (= Y86), Y126 (≠ M120), A255 (= A246)
binding pyridoxal-5'-phosphate: R56 (= R50), K156 (= K149), Y161 (= Y153), E190 (= E182), G193 (= G185), E194 (≠ D186), L214 (= L205), G216 (= G207), I217 (= I208), T218 (= T209), T254 (= T245)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
42% identity, 95% coverage: 3:276/288 of query aligns to 5:285/304 of 1i1mA

query
sites
1i1mA

L

I

Y

W

I

F

D

N

G

G

E

E

F

M

Y

V

S

R

R

W

E

E

D

D

A

A

K

K

I

V

S

H

P

V

F

M

D

S

H

H

G

A

F

L

L

H

Y

Y

G

G

D

T

G

S

V

V

F

F

E

E

G

G

I

I

R

R

A

C

Y

Y

S

D

G

S

R

H

-

K

-

G

-

P

-

V

V

V

F

F

R

R

L

H

S

R

E

E

H

H

L

M

D

Q

R
|
R

L

L

Y

H

D

D

S

S

A

A

K

K

T

I

I

Y

D

R

L

F

Q

P

I

V

P

S

L

Q

S

S

K

I

E

D

E

E

M

L

T

M

E

E

A

A

I

C

L

R

E

D

T

V

C

I

R

R

K

K

N

N

L

L

K

T

D

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

V

I

S

F

R

V

G

G

V

D

G

V

D

G

L

M

G

G

L

V

D

N

P

P

R

P

K

A

C

G

E

Y

K

S

P

T

T

D

V

V

V

I

F

A

A

A

V

F

E

P

W

W

G

G

A

A

M

Y

Y

L

G

G

-

A

D

E

L

A

Y

L

E

E

K

Q

G

G

L

I

K

D

A

A

I

M

S

V

V

S

S

S

I

W

R

N

R

R

N

A

P

A

A

P

E

N

S

T

L

I

P

P

P

T

N

A

V

A

K
|
K

S

A

L

G

-

G

N

N

Y
|
Y

L

L

N

S

I

L

L

L

A

V

K

G

I

S

E

E

A

A

N

R

Y

R

K

H

G

G

G

Y

D

Q

E

E

A

G

I

I

F

A

F

L

D

D

T

V

N

N

G

G

Y

Y

V

I

S

S

E
|
E

G

G

S

A

G
|
G

D
x
E

N

N

I

L

F

F

V

E

V

V

K

K

N

D

G

G

S

V

I

L

S

F

T

T

P

P

-

F

T

T

I

S

N

S

N

A

L
|
L

R

P

G
|
G

I
|
I

T
|
T

R

R

L

D

V

A

G

I

I

I

E

K

I

L

A

A

N

K

E

E

L

L

G

G

I

I

N

E

I

V

S

R

E

E

R

Q

N

V

M

L

G

S

Y

R

Y

E

D

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

V

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

A

T

P

P

I

V

T

R

L

S

I

V

D

D

G

G

R

I

V

Q

I

V

G

G

N

E

G

G

K

R

P

C

G

G

A

P

I

V

T

T

R

K

Q

R

M

I

M

Q

E

Q

K

A

F

F

active site: K156 (= K149)
binding 4-methyl valeric acid: Y92 (= Y86), K156 (= K149), T254 (= T245), A255 (= A246)
binding pyridoxal-5'-phosphate: R56 (= R50), K156 (= K149), Y161 (= Y153), E190 (= E182), G193 (= G185), E194 (≠ D186), L214 (= L205), G216 (= G207), I217 (= I208), T218 (= T209), G253 (= G244), T254 (= T245)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
42% identity, 95% coverage: 3:276/288 of query aligns to 5:285/304 of 1i1lA

query
sites
1i1lA

L

I

Y

W

I

F

D

N

G

G

E

E

F

M

Y

V

S

R

R

W

E

E

D

D

A

A

K

K

I

V

S

H

P

V

F

M

D

S

H

H

G

A

F

L

L

H

Y

Y

G

G

D

T

G

S

V

V

F

F

E

E

G

G

I

I

R

R

A

C

Y

Y

S

D

G

S

R

H

-

K

-

G

-

P

-

V

V

V

F

F

R

R

L

H

S

R

E

E

H

H

L

M

D

Q

R
|
R

L

L

Y

H

D

D

S

S

A

A

K

K

T

I

I

Y

D

R

L

F

Q

P

I

V

P

S

L

Q

S

S

K

I

E

D

E

E

M

L

T

M

E

E

A

A

I

C

L

R

E

D

T

V

C

I

R

R

K

K

N

N

L

L

K

T

D

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

V

I

S

F

R

V

G

G

V

D

G

V

D

G

L

M

G

G

L

V

D

N

P

P

R

P

K

A

C

G

E

Y

K

S

P

T

T

D

V

V

V

I

F

A

A

A

V

F

E

P

W

W

G

G

A

A

M

Y

Y

L

G

G

-

A

D

E

L

A

Y

L

E

E

K

Q

G

G

L

I

K

D

A

A

I

M

S

V

V

S

S

S

I

W

R

N

R

R

N

A

P

A

A

P

E

N

S

T

L

I

P

P

P

T

N

A

V

A

K
|
K

S

A

L

G

-

G

N

N

Y
|
Y

L

L

N

S

I

L

L

L

A

V

K

G

I

S

E

E

A

A

N

R

Y

R

K

H

G

G

G

Y

D

Q

E

E

A

G

I

I

F

A

F

L

D

D

T

V

N

N

G

G

Y

Y

V

I

S

S

E
|
E

G

G

S

A

G
|
G

D

E

N

N

I

L

F

F

V

E

V

V

K

K

N

D

G

G

S

V

I

L

S

F

T

T

P

P

-

F

T

T

I

S

N

S

N

A

L

L

R

P

G
|
G

I
|
I

T
|
T

R

R

L

D

V

A

G

I

I

I

E

K

I

L

A

A

N

K

E

E

L

L

G

G

I

I

N

E

I

V

S

R

E

E

R

Q

N

V

M

L

G

S

Y

R

Y

E

D

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

V

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

I

I

A

T

P

P

I

V

T

R

L

S

I

V

D

D

G

G

R

I

V

Q

I

V

G

G

N

E

G

G

K

R

P

C

G

G

A

P

I

V

T

T

R

K

Q

R

M

I

M

Q

E

Q

K

A

F

F

active site: K156 (= K149)
binding 2-methylleucine: Y92 (= Y86), K156 (= K149), T254 (= T245), A255 (= A246)
binding pyridoxal-5'-phosphate: R56 (= R50), K156 (= K149), Y161 (= Y153), E190 (= E182), G193 (= G185), G216 (= G207), I217 (= I208), T218 (= T209), T254 (= T245)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
41% identity, 100% coverage: 1:288/288 of query aligns to 7:301/301 of 6thqB

query
sites
6thqB

M

M

Q

K

L

V

Y

W

I

L

D

D

G

G

E

R

F

L

Y

V

S

D

R

E

E

E

D

E

A

A

K

K

I

V

S

T

P

V

F

L

D

S

H

P

G

S

F

L

L

N

Y

Y

G

G

D

F

G

G

V

V

F
|
F

E

E

G

G

I

I

R

R

A

A

Y

Y

S

W

G

N

-

G

-

E

-

N

-

L

R

Y

V

V

F

F

R

R

L

L

S

R

E

D

H

H

L

M

D

E

R
|
R

L

L

Y

L

D

R

S

S

A

A

K

K

T

I

I

I

D

G

L

L

Q

D

I

V

P

P

L

Y

S

T

K

A

E

E

M

L

T

S

E

K

A

A

I

V

L

V

E

E

T

T

C

V

R

R

K

A

N

N

N

G

L

F

K

K

-

E

D

D

A

L

Y
|
Y

I

I

R

R

P

P

L

V

V

A

S

Y

R

I

G

S

V

K

G

P

D

Q

L

I

G

S

L

L

D

D

P

V

R

R

K

G

C

L

E

Q

K

A

P

-

T

S

V

V

V

A

V

I

F

A

A

A

V

I

E

P

W

F

G

G

A

K

M

Y

Y

-

G

-

D

-

L

L

Y

K

E

V

K

E

G

G

L

V

K

R

A

A

I

A

S

V

V

V

S

S

I

W

R

R

N

V

P

H

A

T

E

S

S

M

L

M

P

P

P

V

N

M

V

A

K
|
K

S

A

L

T

N

G

-

I

Y
|
Y

L

L

N

N

N

S

I

I

L

M

A

A

K

A

I

V

E

E

A

A

N

R

Y

A

K

R

G

G

G

Y

D

D

E

E

A

A

I

I

F

M

F

L

D

N

T

A

N

E

G

G

Y

K

V

V

S

V

E
|
E

G

G

S

S

G
|
G

D
x
E

N
|
N

I

I

F

F

V

I

V

V

K

R

N

R

G

G

S

V

I

L

S

M

T

T

P

P

T

L

I

E

N

D

N

G

-

I

L
|
L

R

E

G
|
G

I
|
I

T
|
T

R

R

L

E

V

T

G

V

I

I

E

S

I

I

A

A

N

G

E

D

L

L

G

G

I

I

N

P

I

L

S

L

E

E

R

K

N

S

M

I

G

T

Y

R

Y

E

D

E

L

L

Y

Y

S

A

A

A

D

D

E

E

V

A

F

F

V

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

A

T

P

P

I

I

T

I

L

E

I

I

D

D

G

G

R

R

V

V

I

L

G

-

N

-

G

-

K

Q

P

R

G

G

A

P

I

I

T

T

R

Q

Q

K

M

I

M

A

E

E

K

T

F

Y

K

R

I

R

V

I

V

V

Q

L

N

G

E

K

G

E

-

E

-

K

-

Y

-

L

-

P

-

W

-

L

T

T

P

P

I

V

Y

Y

active site: F37 (= F31), K156 (= K149), E190 (= E182), L214 (= L205)
binding pyridoxal-5'-phosphate: R60 (= R50), K156 (= K149), Y161 (= Y153), E190 (= E182), N195 (= N187), L214 (= L205), G216 (= G207), I217 (= I208), T218 (= T209), T254 (= T245)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R50), Y97 (= Y86), K156 (= K149), Y161 (= Y153), E190 (= E182), G193 (= G185), E194 (≠ D186), N195 (= N187), G216 (= G207), I217 (= I208), T218 (= T209), G253 (= G244), T254 (= T245), A255 (= A246)

7p3tB Transaminase of gamma-proteobacterium (see paper)
39% identity, 99% coverage: 3:287/288 of query aligns to 6:292/299 of 7p3tB

query
sites
7p3tB

L

I

Y

Y

I

I

D

N

G

G

E

D

F

Y

Y

L

S

P

R

L

E

S

D

Q

A

A

K

R

I

V

S

S

P

P

F

V

D

D

H

Q

G

G

F

F

L

L

Y

L

G

G

D

D

G

G

V

V

F

F

E

D

G

V

I

V

R

S

A

A

Y

W

S

K

G

G

R

N

V

I

F

F

R

K

L

L

S

D

E

A

H

H

L

L

D

D

R
|
R

L

F

Y

F

D

D

S

S

A

I

K

Q

T

A

I

A

D

R

L

L

Q

N

I

H

P

D

L

M

S

S

K

R

E

D

E

A

M

W

T

K

E

E

A

A

I

I

L

I

E

E

T

T

C

T

R

R

K

R

N

N

N

G

L

L

K

D

D

D

A

A

Y

S

I

I

R

R

P

F

L

I

V

V

S

T

R

R

G

G

V

E

G

P

D

K

-

G

L

V

G

V

L

A

D

D

P

P

R

R

K

D

C

F

E

-

K

K

P

P

T

T

V

C

V

I

V

V

F

-

A

-

V

-

E

-

W

W

G

V

A

A

M

P

Y

Y

G

I

D

F

L

L

Y

A

E

D

K

E

-

E

-

K

-

R

-

N

G

G

L

I

K

R

A

L

I

M

S

I

V

S

S

A

I

T

R

R

R

G

N

F

P

P

A

A

E

D

S

T

L

L

P

D

P

P

N

R

V

Y

K
|
K

S

C

L

L

N

D

Y
x
R

L

L

N

H

N

S

I

Q

L

L

A

I

K

R

I

L

E

E

A

A

N

L

Y

E

K

A

G

G

G

Y

D

D

E

D

A

A

I

L

F

W

F

L

D

D

T

H

N

S

G

G

Y

H

V

V

S

S

E
|
E

G
x
S

S
x
A

G
x
A

D
x
S

N

N

I

L

F

F

V

I

V

V

K

K

N

N

G

G

S

V

I

L

S

Y

T

T

P

-

P

P

T

S

I

A

N

G

N

I

L

L

R

R

G
|
G

I
|
I

T
|
T

R

R

L

D

V

T

G

I

I

L

E

E

I

L

A

A

N

T

E

E

L

L

G

D

I

I

N

P

I

W

S

K

E

E

R

R

N

Q

M

L

G

S

Y

A

Y

F

D

D

L

V

Y

Y

S

I

A

A

D

D

E

E

V

V

F

F

V

T

T

C

G

S

T
|
T

A

A

A

G

E

G

I

A

A

L

P

P

I

V

T

R

L

E

I

V

D

A

G

G

R

R

V

T

I

I

G

R

N

G

G

T

K

T

P

P

G

G

A

P

I

I

T

T

R

Q

Q

A

M

I

M

D

E

N

K

A

F

Y

K

W

I

A

V

M

V

R

Q

E

N

T

E

D

-

R

-

Y

G

A

T

T

P

P

I

L

binding pyridoxal-5'-phosphate: R53 (= R50), K153 (= K149), R157 (≠ Y153), E186 (= E182), S187 (≠ G183), A188 (≠ S184), A189 (≠ G185), S190 (≠ D186), G210 (= G207), I211 (= I208), T212 (= T209), T248 (= T245)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
40% identity, 97% coverage: 2:280/288 of query aligns to 8:292/306 of 4whxA

query
sites
4whxA

Q

K

L

I

Y

W

I

M

D

D

G

G

E

K

F

L

Y

I

S

E

R

W

E

R

D

D

A

A

K

K

I

I

S

H

P

V

F

L

D

T

H

H

G

T

F

L

L

H

Y

Y

G

G

D

M

G

G

V

V

F
|
F

E

E

G

G

I

V

R

R

A

A

Y

Y

S

K

G

T

R

A

-

D

-

G

-

T

-

A

V

I

F

F

R

R

L

L

S

K

E

E

H

H

L

T

D

K

R

R

L

L

Y

L

D

N

S

S

A

A

K

K

T

I

I

F

D

Q

L

M

Q

D

I

V

P

P

L

F

S

D

K

Q

E

E

E

T

M

L

T

E

E

A

A

A

I

Q

L

R

E

D

T

V

C

V

R

R

K

E

N

N

N

K

L

L

K

E

D

S

A

C

Y
|
Y

I

L

R

R

P

P

L

I

V

I

S

W

R

I

G

G

V

S

G

E

D

K

L

L

G

G

L

V

D

S

P

A

R

-

K

K

C

G

E

N

K

T

P

I

T

H

V

V

V

A

V

I

F

A

A

A

V

W

E

P

W

W

G

G

A

A

M

Y

Y

L

G

G

D

E

L

E

-

G

Y

L

E

A

K

K

G

G

L

I

K

R

A

V

I

K

S

T

V

S

S

S

I

F

R

T

R

R

N

H

P

H

A

V

E

N

-

V

S

S

L

M

P

V

P

R

N

A

V
x
K

K

A

S

S

L

G

N

W

Y

Y

L

V

N

N

N

S

I

I

L

L

A

A

K

N

I

Q

E

E

A

A

N

T

Y

A

K

D

G

G

G

Y

D

D

E

E

A

A

I

L

F

L

D

D

T

V

N

D

G

G

Y

Y

V

V

S

S

E
|
E

G

G

S

S

G

G

D

E

N

N

I

F

F

F

V

L

V

V

K

N

N

R

G

G

S

K

I

L

S

Y

T

T

P

P

P

D

T

L

I

A

N

S

N

C

L
|
L

R

D

G

G

I

I

T

T

R

R

L

D

V

T

G

V

I

I

E

T

I

L

A

A

N

K

E

E

L

A

G

G

I

I

N

E

I

V

S

I

E

E

R

K

N

R

M

I

G

T

Y

R

Y

D

D

E

L

V

Y

Y

S

T

A

A

D

D

E

E

V

A

F

F

V

F

T

T

G

G

T

T

A
|
A

A

A

E

E

I

V

A

T

P

P

I

I

T

R

L

E

I

L

D

D

G

N

R

R

V

T

I

I

G

G

N

G

G

G

K

A

P

R

G

G

A

P

I

I

T

T

R

E

Q

K

M

L

M

Q

E

S

K

A

F

F

K

F

I

D

V

V

active site: F37 (= F31), K160 (≠ V148), E194 (= E182), L217 (= L205)
binding alanine: Y97 (= Y86), K160 (≠ V148), A258 (= A246)

7z79B Crystal structure of aminotransferase-like protein from variovorax paradoxus
35% identity, 90% coverage: 3:260/288 of query aligns to 18:277/306 of 7z79B

query
sites
7z79B

L

V

Y

Y

I

V

D

N

G

G

E

Q

F

F

Y

V

S

P

R

R

E

H

D

Q

A

A

K

V

I

V

S

S

P

V

F

F

D

D

H

A

G

G

F

Y

L

V

Y

C

G

G

D

D

G

G

V

V

F
x
W

E

E

G

G

I

V

R

R

A

L

Y

V

S

D

G

G

R

R

V

I

F

V

R

S

L

F

S

D

E

A

H

H

L

I

D

D

R
|
R

L

M

Y

Y

D

E

S

G

A

A

K

K

T

S

I

I

D

A

L

L

Q

D

I

I

P

G

L

M

S

T

K

R

E

A

E

Q

M

T

T

K

E

Q

A

V

I

V

L

V

E

D

T

T

C

F

R

L

K

R

N

N

N

G

L

M

K

R

D

D

-

G

A

A

Y

H

I

A

R

R

P

L

L

M

V

V

S

T

R

R

G

G

V

V

G

K

D

K

L

T

-

P

G

N

L

Q

D

D

P

P

R

R

K

F

C

I

E

I

K

G

P

G

T

A

V

T

V

V

F

C

A

V

V

A

E

E

W

H

G

K

A

V

M

V

Y

T

G

P

D

E

L

A

Y

K

E

R

K

N

G

G

L

L

K

K

A

L

I

F

S

T

V

S

S

T

I

L

R

R

R

C

N

S

P

G

A

P

E

D

S

V

L

F

P

D

P

L

N

R

V

L

K
x
N

S

S

L

H

N

S

Y

R

L

L

N

N

N

L

I

I

L

Q

A

A

K

L

I

I

E

Q

A

A

N

I

Y

Q

K

A

G

G

G

A

D

D

E

E

A

A

I

L

F

M

F

L

D

D

T

P

N

N

G

G

Y

F

V

V

S

S

E

S

G

C

S

N

G
x
S

D
x
T

N

N

I

F

F

F

V

A

V

V

K

R

N

N

G

G

S

A

I

L

S

W

T

T

P

S

T

G

I

R

N

Y

N

C

L
x
F

R

N

G
|
G

I
|
I

T
|
T

R

R

L

A

V

T

G

V

I

V

E

R

I

L

A

A

N

R

E

E

L

A

G

G

I

I

N

P

I

V

S

H

E

E

R

G

N

D

M

F

G

T

Y

L

Y

A

D

E

L

V

Y

Y

S

A

A

A

D

D

E

E

V

A

F

F

V

V

T

T

G
|
G

T
|
T

A

L

A

A

E

G

I

L

A

T

P

P

I

V

T

S

L

S

I

V

D

D

G

G

R

R

V

A

I

L

binding pyridoxal-5'-phosphate: W46 (≠ F31), R65 (= R50), N166 (≠ K149), S202 (≠ G185), T203 (≠ D186), F222 (≠ L205), G224 (= G207), I225 (= I208), T226 (= T209), G261 (= G244), T262 (= T245)

P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
39% identity, 92% coverage: 13:277/288 of query aligns to 14:274/283 of P19938

query
sites
P19938

E

E

D

E

A

V

K

K

I

I

S

D

P

K

F

E

D

D

H

R

G

G

F

Y

L

Q

Y

F

G

G

D

D

G

G

V

V

F
x
Y

E

E

G

V

I

V

R

K