SitesBLAST

7ysmA Crystal structure of udp-glucose 4-epimerase (rv3634c) co-crystallized with udp-n-acetylglucosamine from mycobacterium tuberculosis
35% identity, 66% coverage: 1:207/312 of query aligns to 1:212/311 of 7ysmA

query
sites
7ysmA

M

M

R

R

S

A

I

L

V

V

T

T

G
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G

G

A

A

A

G
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G

F
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F

I
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I

G

G

S

S

H

T

I

L

V

V

D

D

H

R

L

L

V

L

A

A

Q

D

G

G

D

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E

S

V

V

V

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I

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D

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x
N

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F

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G

D

R

L

A

R

T

F

N

I

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E

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H

H

L

L

A

D

D

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G

S

A

A

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K

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F

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A

A

N
x
D

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x
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P

A

G

D

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L

Q

H

D

A

V

I

C

L

A

E

G

Q

-

H

-

R

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D

E

R

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V

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W

F

H

H

I
x
L

A
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A

D

Q

P
x
I

D

D

V

V

R
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R

E

R

S

S

A

V

V

A

T

D

P

P

D

Q

A

F

Q

D

L

A

N

A

N

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T

N

I

V

I

I

A

G

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T

H

V

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V

L

L

A

E

E

A

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I

A

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G

I

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R

E

K

I

I

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V

F

H

T
|
T

S

S

T
x
S

-

G

S
x
G

T
x
S

V

I

Y

Y

G

G

E

T

A

P

T

P

V

E

I

Y

P

P

T

T

P

P

E

E

T

T

Y

-

T

A

P

P

M

T

E

D

P

P

V

A

S

S

V

P

Y
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Y

G

A

A

A

A

G

K
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K

L

V

A

A

C

G

E

E

A

I

L

Y

I

L

S

N

A

T

Y

F

C

R

H

H

S

L

F

Y

G

G

V

L

R

D

G

C

Y

S

I

H

F

I

R

A

F
x
P

A
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A

N
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N

I
x
V

I

Y

G

G

P

P

R

R

S

Q

G

D

H

P

-

H

-

G

-

E

-

A

G
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G

V
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V

I

V

T

A

D

I

F
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F

I

A

K

Q

K

A

L

L

R

L

A

S

N

G

P

-

K

K

E

P

L

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E
x
R

I
x
V

L
x
F

G

G

D

D

G

G

K

T

Q
x
N

T

T

binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), N32 (≠ A32), T35 (≠ A35), G36 (= G36), D56 (≠ N56), I57 (≠ L57), L77 (≠ I75), A78 (= A76), A79 (= A77), I81 (≠ P79), T119 (= T117), Y146 (= Y144), K150 (= K148), P173 (≠ F171), N175 (= N173), V176 (≠ I174)
binding uridine-diphosphate-n-acetylgalactosamine: I81 (≠ P79), R84 (= R82), S121 (≠ T119), G123 (≠ S120), S124 (≠ T121), Y146 (= Y144), A174 (= A172), N175 (= N173), G188 (= G182), V189 (= V183), F193 (= F187), R204 (≠ E199), V205 (≠ I200), F206 (≠ L201), N211 (≠ Q206)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylgalactosamine: 213, 248, 271

7ystA Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chain b from mycobacterium tuberculosis
35% identity, 66% coverage: 1:207/312 of query aligns to 1:212/312 of 7ystA

query
sites
7ystA

M

M

R

R

S

A

I

L

V

V

T

T

G
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G

G

A

A

A

G
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G

F
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F

I
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I

G

G

S

S

H

T

I

L

V

V

D

D

H

R

L

L

V

L

A

A

Q

D

G

G

D

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E

S

V

V

V

G

I

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D
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D

A
x
N

L

F

L

A

A
x
T

G
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G

D

R

L

A

R

T

F

N

I

L

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E

R

H

H

L

L

A

D

D

S

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G

S

A

A

V

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F

I

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E

A

A

N
x
D

L
x
I

L

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T

P

A

G

D

W

L

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D

A

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C

L

A

E

G

Q

-

H

-

R

A

P

D

E

R

V

V

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W

F

H

H

I
x
L

A
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A

D

Q

P
x
I

D

D

V

V

R
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R

E

R

S

S

A

V

V

A

T

D

P

P

D

Q

A

F

Q

D

L

A

N

A

N
x
V

T

N

I

V

I

I

A

G

T

T

H

V

R

R

V

L

L

A

E

E

A

A

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T

K

G

I

V

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R

E

K

I

I

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V

F

H

T
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T

S

S

T
x
S

-

G

S
x
G

T

S

V

I

Y

Y

G

G

E

T

A

P

T

P

V

E

I

Y

P

P

T

T

P

P

E

E

T

T

Y

-

T

A

P

P

M

T

E

D

P

P

V

A

S

S

V

P

Y
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Y

G

A

A

A

A

G

K
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K

L

V

A

A

C

G

E

E

A

I

L

Y

I

L

S

N

A

T

Y

F

C

R

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H

S

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F

Y

G

G

V

L

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S

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H

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x
P

A
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A

N
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N

I
x
V

I

Y

G

G

P

P

R

R

S

Q

G

D

H

P

-

H

-

G

-

E

-
x
A

G
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G

V
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V

I

V

T

A

D

I

F
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F

I

A

K

Q

K

A

L

L

R

L

A

S

N

G

P

-

K

K

E

P

L

T

E
x
R

I
x
V

L
x
F

G

G

D

D

G

G

K

T

Q

N

T

T

binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), N32 (≠ A32), T35 (≠ A35), G36 (= G36), D56 (≠ N56), I57 (≠ L57), L77 (≠ I75), A78 (= A76), A79 (= A77), I81 (≠ P79), V96 (≠ N94), T119 (= T117), Y146 (= Y144), K150 (= K148), P173 (≠ F171), A174 (= A172), N175 (= N173), V176 (≠ I174)
binding uridine-5'-diphosphate-glucose: I81 (≠ P79), R84 (= R82), S121 (≠ T119), G123 (≠ S120), Y146 (= Y144), A174 (= A172), N175 (= N173), A187 (vs. gap), G188 (= G182), V189 (= V183), F193 (= F187), R204 (≠ E199), V205 (≠ I200), F206 (≠ L201)

Sites not aligning to the query:

binding uridine-5'-diphosphate-glucose: 213, 248, 271

7ys9A Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chaina from mycobacterium tuberculosis
35% identity, 66% coverage: 1:207/312 of query aligns to 1:212/310 of 7ys9A

query
sites
7ys9A

M

M

R

R

S

A

I

L

V

V

T

T

G
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G

G

A

A

A

G
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G

F
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F

I
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I

G

G

S

S

H

T

I

L

V

V

D

D

H

R

L

L

V

L

A

A

Q

D

G

G

D

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D

A
x
N

L

F

L
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A

A
x
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G

D

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A

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F

N

I

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R

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H

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L

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D

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G

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A

A

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K

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F

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A

N
x
D

L
x
I

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P

A

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W

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G

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F

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H

I
x
L

A
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A

D

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x
I

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R

E

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S

A

V

V

A

T

D

P

P

D

Q

A

F

Q

D

L

A

N

A

N

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N

I

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I

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G

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H

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L

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K

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H

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T

S

S

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x
S

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G

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x
G

T

S

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I

Y

Y

G

G

E

T

A

P

T

P

V

E

I

Y

P

P

T

T

P

P

E

E

T

T

Y

-

T

A

P

P

M

T

E

D

P

P

V

A

S

S

V

P

Y
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Y

G

A

A

A

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K
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K

L

V

A

A

C

G

E

E

A

I

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Y

I

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N

A

T

Y

F

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F

Y

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G

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F
x
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A
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A

N
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N

I
x
V

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Y

G

G

P

P

R

R

S

Q

G

D

H

P

-

H

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G

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E

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x
A

G
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G

V
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V

I

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T

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D

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F
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F

I

A

K

Q

K

A

L

L

R

L

A

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N

G

P

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K

K

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E
x
R

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x
F

G

G

D

D

G

G

K

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x
N

T

T

binding galactose-uridine-5'-diphosphate: I81 (≠ P79), R84 (= R82), S121 (≠ T119), G123 (≠ S120), Y146 (= Y144), A174 (= A172), N175 (= N173), A187 (vs. gap), G188 (= G182), V189 (= V183), F193 (= F187), R204 (≠ E199), F206 (≠ L201), N211 (≠ Q206)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), N32 (≠ A32), A34 (≠ L34), T35 (≠ A35), G36 (= G36), D56 (≠ N56), I57 (≠ L57), L77 (≠ I75), A78 (= A76), A79 (= A77), I81 (≠ P79), T119 (= T117), Y146 (= Y144), K150 (= K148), P173 (≠ F171), A174 (= A172), V176 (≠ I174)
binding uridine-5'-diphosphate-glucose: I81 (≠ P79), R84 (= R82), S121 (≠ T119), G123 (≠ S120), Y146 (= Y144), A174 (= A172), N175 (= N173), A187 (vs. gap), G188 (= G182), V189 (= V183), F193 (= F187), R204 (≠ E199), F206 (≠ L201), N211 (≠ Q206)

Sites not aligning to the query:

2p5uA Crystal structure of thermus thermophilus hb8 udp-glucose 4-epimerase complex with NAD
31% identity, 97% coverage: 1:304/312 of query aligns to 1:304/311 of 2p5uA

query
sites
2p5uA

M

M

R

R

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V

T

T

G

G

A

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G

F
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F

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I

G

G

S

S

H

H

I

I

V

V

D

E

H

D

L

L

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A

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G

D

L

E

E

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V

V

A

V

V

I

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D
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D

A
x
N

L
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L

L
x
A

A
x
T

G
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G

D

K

L

R

R

E

F

N

I

V

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P

R

K

H

-

L

-

D

-

S

-

G

-

A

G

V

V

K

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F

F

I

F

R

R

A

V

N
x
D

L
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L

-

R

-

D

-

K

-

E

-

G

L

V

E

E

P

R

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F

C

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A

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G

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A

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H

V

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W

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H

H

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x
Q

A
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A

D

Q

P
x
A

D

S

V

V

R

K

E

V

S

S

A

V

V

E

T

D

P

P

D

V

A

L

Q

D

L

F

N

E

N

V

T

N

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T

G

H

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L

L

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A

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V

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S

T
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T

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G

S
x
G

T
x
A

V

I

Y

Y

G

G

E

E

A

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V

E

I

G

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Y

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P

M

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E

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S

S

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Y
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Y

G

A

A

A

A

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K
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K

L

A

A

A

C

F

E

E

A

H

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Y

I

L

S

S

A

V

Y

Y

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Y

G

G

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R

K

G

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Y

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F

L

R

R

F

Y

A

G

N

N

I
x
V

I

Y

G

G

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P

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R

S

Q

G

D

H

P

-
x
H

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G

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E

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A

G
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G

V

V

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V

T

A

D

I

F

F

I

A

K

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K

R

-

V

L

L

R

K

A

G

N

L

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P

K

V

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T

L

L

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Y

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A

-

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P

G

G

D

D

G

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K

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Y

Y

L

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Y

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G

A

D

C

V

V

A

A

E

A

A

I

H

S

A

Y

L

V

A

I

L

A

F

H

S

A

L

N

E

D

-

Q

-

V

-

N

G

T

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Y

Y

N

N

I

V

G

G

S

T

E

G

D

E

W

G

I

-

D

-

V

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T

H

T

T

I

T

A

R

D

E

I

V

L

L

V

M

K

A

E

V

A

A

G

E

L

A

A

A

G

G

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S

A

Y

P

K

E

Y

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Q

G

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G

A

S

P

R

P

G

-

W

R

V

P

G

G

D

D

V

L

P

E

K

R

M

S

Q

V

L

L

S

S

V

P

E

L

K

K

L

L

K

M

S

A

L

H

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G

Y

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active site: T117 (= T119), G119 (≠ S120), A120 (≠ T121), Y143 (= Y144), K147 (= K148), H181 (vs. gap), G185 (= G182)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), N32 (≠ A32), L33 (= L33), A34 (≠ L34), T35 (≠ A35), G36 (= G36), D51 (≠ N56), L52 (= L57), Q73 (≠ I75), A74 (= A76), A75 (= A77), A77 (≠ P79), S116 (= S118), Y143 (= Y144), K147 (= K148), V173 (≠ I174)

6zllA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-galacturonic acid and NAD (see paper)
32% identity, 82% coverage: 1:256/312 of query aligns to 1:262/321 of 6zllA

query
sites
6zllA

M

M

R

K

S

I

I

L

V

V

T

T

G
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G

G

A

A

A

G
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G

F
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F

I
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I

G

G

S

S

H

H

I

L

V

C

D

Q

H

A

L

L

V

L

A

K

Q

N

G

S

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A

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Y

E

H

V

V

V

G

I

I

D
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D

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x
H

-
x
F

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x
I

-

G

-

P

-

T

-

P

-

A

A

T

L

L

L
x
K

A

T

G

G

D

N

L

I

R

Q

F

-

I

-

S

S

R

L

H

E

L

L

D

N

S

S

G

-

A

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K

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F

F

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R

R

A

E

N
x
D

L
x
I

L

L

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N

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D

K

V

L

C

L

A

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D

A

I

D

D

R

V

V

V

W

Y

H

H

I
x
L

A
|
A

D

I

P
|
P

D

G

V
|
V

R
|
R

E

T

S

S

A

W

V

-

T

G

P

K

D

D

A

F

Q

Q

-

P

-

Y

L

V

N

T

N

N

T

N

I

I

I

M

A

V

T

T

H

Q

R

Q

V

L

L

L

E

E

A

A

I

C

R

K

L

H

H

I

K

K

I

L

P

D

E

K

I

F

V

I

F

H

T
x
I

S

S

T
|
T

S
|
S

T
x
S

V

V

Y

Y

G

G

E

E

A

K

T

S

V

G

I

-

P

A

T

V

P

S

E

E

T

D

Y

L

T

L

P

P

M

I

E

-

P

P

V

L

S

S

V

P

Y
|
Y

G

G

A

V

A

T

K
|
K

L

L

A

S

C

G

E

E

A

H

L

L

I

C

S

H

A

V

Y

Y

C

H

H

K

S

N

F

F

G

H

V

I

R

P

G

I

Y

V

I

I

F

L

R

R

F
x
Y

A

F

N
x
T

I
x
V

I

Y

G

G

P

P

R

R

S

Q

G
x
R

H

P

G

D

V
x
M

I
x
A

T

F

D

H

F
x
R

I

L

K

I

K

K

L

Q

R

M

A

L

N

E

P

D

K

K

E

P

L

L

E
x
T

I

I

L
x
F

G

G

D

D

G

G

K

T

Q
|
Q

T

T

K
x
R

S

D

Y

F

L

T

E

Y

V

I

T

D

A

D

C

C

V

I

-

R

A

G

A

T

I

V

S

A

Y

A

V

L

I

E

A

T

H

K

A

K

N

N

D

I

Q

I

V

G

N

E

T

V

Y

I

N

N

I

I

G

G

S

G

E

K

D

E

W

Q

I

A

D

S

V
x
I

T

L

T

D

I

I

A

I

D

S

I

M

L

L

V

E

K

K

E

I

A

S

G

G

active site: T126 (= T119), S127 (= S120), S128 (≠ T121), Y149 (= Y144), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (vs. gap), F34 (vs. gap), I35 (vs. gap), K43 (≠ L34), D62 (≠ N56), I63 (≠ L57), L81 (≠ I75), A82 (= A76), A83 (= A77), I124 (≠ T117), T126 (= T119), Y149 (= Y144), K153 (= K148), Y176 (≠ F171), V179 (≠ I174), R185 (≠ G180), M188 (≠ V183)
binding (2S,3R,4S,5R,6R)-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4,5-trihydroxy-oxane-2-carboxylic acid: P85 (= P79), V87 (= V81), R88 (= R82), T126 (= T119), S127 (= S120), Y149 (= Y144), T178 (≠ N173), R185 (≠ G180), A189 (≠ I184), R192 (≠ F187), T204 (≠ E199), F206 (≠ L201), Q211 (= Q206), R213 (≠ K208), I250 (≠ V244)

Sites not aligning to the query:

binding (2S,3R,4S,5R,6R)-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4,5-trihydroxy-oxane-2-carboxylic acid: 276

6zldA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-glucuronic acid and NAD (see paper)
32% identity, 82% coverage: 1:256/312 of query aligns to 1:262/314 of 6zldA

query
sites
6zldA

M

M

R

K

S

I

I

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

I

L

V

C

D

Q

H

A

L

L

V

L

A

K

Q

N

G

S

D

A

-

Y

E

H

V

V

V

G

I

I

D
|
D

-
x
H

-
x
F

-
x
I

-

G

-

P

-

T

-

P

-

A

A

T

L

L

L
x
K

A

T

G

G

D

N

L

I

R

Q

F

-

I

-

S

S

R

L

H

E

L

L

D

N

S

S

G

-

A

R

V

F

K

Q

F

F

I

I

R

R

A

E

N
x
D

L
x
I

L

L

E

N

P

T

G

D

W

L

Q

S

D

K

V

L

C

L

A

Q

G

D

A

I

D

D

R

V

V

V

W

Y

H

H

I
x
L

A
|
A

D

I

P
|
P

D

G

V

V

R
|
R

E

T

S

S

A

W

V

-

T

G

P

K

D

D

A

F

Q

Q

-

P

-

Y

L

V

N

T

N

N

T

N

I

I

I

M

A

V

T

T

H

Q

R

Q

V

L

L

L

E

E

A

A

I

C

R

K

L

H

H

I

K

K

I

L

P

D

E

K

I

F

V

I

F

H

T
x
I

S

S

T
|
T

S
|
S

T
x
S

V

V

Y

Y

G

G

E

E

A

K

T

S

V

G

I

-

P

A

T

V

P

S

E

E

T

D

Y

L

T

L

P

P

M

I

E

-

P

P

V

L

S

S

V

P

Y
|
Y

G

G

A

V

A

T

K
|
K

L

L

A

S

C

G

E

E

A

H

L

L

I

C

S

H

A

V

Y

Y

C

H

H

K

S

N

F

F

G

H

V

I

R

P

G

I

Y

V

I

I

F

L

R

R

F
x
Y

A
x
F

N
x
T

I

V

I

Y

G

G

P

P

R

R

S

Q

G
x
R

H

P

G

D

V
x
M

I
x
A

T

F

D

H

F
x
R

I

L

K

I

K

K

L

Q

R

M

A

L

N

E

P

D

K

K

E

P

L

L

E
x
T

I

I

L
x
F

G

G

D

D

G

G

K

T

Q
|
Q

T

T

K
x
R

S

D

Y

F

L

T

E

Y

V

I

T

D

A

D

C

C

V

I

-

R

A

G

A

T

I

V

S

A

Y

A

V

L

I

E

A

T

H

K

A

K

N

N

D

I

Q

I

V

G

N

E

T

V

Y

I

N

N

I

I

G

G

S

G

E

K

D

E

W

Q

I

A

D

S

V
x
I

T

L

T

D

I

I

A

I

D

S

I

M

L

L

V

E

K

K

E

I

A

S

G

G

active site: T126 (= T119), S127 (= S120), S128 (≠ T121), Y149 (= Y144), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (vs. gap), F34 (vs. gap), I35 (vs. gap), K43 (≠ L34), D62 (≠ N56), I63 (≠ L57), L81 (≠ I75), A82 (= A76), A83 (= A77), I124 (≠ T117), T126 (= T119), K153 (= K148), Y176 (≠ F171), T178 (≠ N173), R185 (≠ G180), M188 (≠ V183)
binding uridine-5'-diphosphate-glucuronic acid: P85 (= P79), R88 (= R82), T126 (= T119), S127 (= S120), S128 (≠ T121), Y149 (= Y144), F177 (≠ A172), T178 (≠ N173), R185 (≠ G180), M188 (≠ V183), A189 (≠ I184), R192 (≠ F187), T204 (≠ E199), F206 (≠ L201), Q211 (= Q206), R213 (≠ K208), I250 (≠ V244)

Sites not aligning to the query:

binding uridine-5'-diphosphate-glucuronic acid: 276

6zl6A Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp and NAD (see paper)
32% identity, 82% coverage: 1:256/312 of query aligns to 1:262/314 of 6zl6A

query
sites
6zl6A

M

M

R

K

S

I

I

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

I

L

V

C

D

Q

H

A

L

L

V

L

A

K

Q

N

G

S

D

A

-

Y

E

H

V

V

V

G

I

I

D
|
D

-
x
H

-
x
F

-
x
I

-

G

-

P

-

T

-

P

-

A

A

T

L

L

L
x
K

A

T

G

G

D

N

L

I

R

Q

F

-

I

-

S

S

R

L

H

E

L

L

D

N

S

S

G

-

A

R

V

F

K

Q

F

F

I

I

R

R

A

E

N
x
D

L
x
I

L

L

E

N

P

T

G

D

W

L

Q

S

D

K

V

L

C

L

A

Q

G

D

A

I

D

D

R

V

V

V

W

Y

H

H

I
x
L

A
|
A

D

I

P

P

D

G

V

V

R

R

E

T

S

S

A

W

V

-

T

G

P

K

D

D

A

F

Q

Q

-

P

-

Y

L

V

N

T

N

N

T

N

I

I

I

M

A

V

T

T

H

Q

R

Q

V

L

L

L

E

E

A

A

I

C

R

K

L

H

H

I

K

K

I

L

P

D

E

K

I

F

V

I

F

H

T
x
I

S

S

T
|
T

S
|
S

T
x
S

V

V

Y

Y

G

G

E

E

A

K

T

S

V

G

I

-

P

A

T

V

P

S

E

E

T

D

Y

L

T

L

P

P

M

I

E

-

P

P

V

L

S

S

V

P

Y
|
Y

G

G

A

V

A

T

K
|
K

L

L

A

S

C

G

E

E

A

H

L

L

I

C

S

H

A

V

Y

Y

C

H

H

K

S

N

F

F

G

H

V

I

R

P

G

I

Y

V

I

I

F

L

R

R

F
x
Y

A

F

N
x
T

I
x
V

I

Y

G

G

P

P

R

R

S

Q

G
x
R

H

P

G

D

V
x
M

I
x
A

T

F

D

H

F
x
R

I

L

K

I

K

K

L

Q

R

M

A

L

N

E

P

D

K

K

E

P

L

L

E
x
T

I

I

L
x
F

G

G

D

D

G

G

K

T

Q
|
Q

T

T

K
x
R

S

D

Y

F

L

T

E

Y

V

I

T

D

A

D

C

C

V

I

-

R

A

G

A

T

I

V

S

A

Y

A

V

L

I

E

A

T

H

K

A

K

N

N

D

I

Q

I

V

G

N

E

T

V

Y

I

N

N

I

I

G

G

S

G

E

K

D

E

W

Q

I

A

D

S

V
x
I

T

L

T

D

I

I

A

I

D

S

I

M

L

L

V

E

K

K

E

I

A

S

G

G

active site: T126 (= T119), S127 (= S120), S128 (≠ T121), Y149 (= Y144), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (vs. gap), F34 (vs. gap), I35 (vs. gap), K43 (≠ L34), D62 (≠ N56), I63 (≠ L57), L81 (≠ I75), A82 (= A76), A83 (= A77), I124 (≠ T117), T126 (= T119), K153 (= K148), Y176 (≠ F171), T178 (≠ N173), V179 (≠ I174), R185 (≠ G180), M188 (≠ V183)
binding uridine-5'-diphosphate: T178 (≠ N173), A189 (≠ I184), R192 (≠ F187), T204 (≠ E199), F206 (≠ L201), Q211 (= Q206), R213 (≠ K208), I250 (≠ V244)

Sites not aligning to the query:

binding uridine-5'-diphosphate: 276

3vpsA Structure of a novel NAD dependent-ndp-hexosamine 5,6-dehydratase, tuna, involved in tunicamycin biosynthesis
33% identity, 84% coverage: 2:263/312 of query aligns to 3:255/303 of 3vpsA

query
sites
3vpsA

R

R

S

I

I

L

V

I

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
|
I

G

G

S

G

H

H

I

L

V

A

D

R

H

A

L

L

V

V

A

A

Q

S

G

G

D

E

E

E

V

V

V

T

V

V

I

L

D
|
D

A

-

L

-

A

-

G

-

D
|
D

L

L

R
|
R

F
x
V

I

P

S

P

R

M

H

I

L

P

D

P

S

E

G

G

A

T

V

G

K

K

F

F

I

L

R

E

A

K

N
x
P

L
x
V

L

L

E

E

P

L

G

E

W

E

Q

R

D

D

V

L

C

-

A

S

G

D

A

V

D

R

R

L

V

V

W

Y

H

H

I
x
L

A
|
A

A
x
S

D

H

P
x
K

D

S

V

V

R

P

E

R

S

S

A

F

V

K

T

Q

P

P

D

L

A

D

Q

Y

L

L

N

D

N

N

T

V

I

D

I

S

A

G

T

R

H

H

R

-

V

L

L

L

E

A

A

L

I

C

R

T

L

S

H

V

K

G

I

V

P

P

E

K

I

V

V

V

F

V

T

G

S

S

T
|
T

S
x
C

T
x
E

V

V

Y

Y

G

G

E

Q

A

A

T

D

V

T

I

L

P

P

T

T

P

P

E

E

T

D

Y

-

T

S

P

P

M

L

E

S

P

P

V

R

S

S

V

P

Y
|
Y

G

A

A

A

A

S

K
|
K

L

V

A

G

C

L

E

E

A

M

L

V

I

A

S

G

A

A

Y

H

C

Q

H

R

-

A

S

S

F

V

G

A

V

P

R

E

G

V

Y

G

I

I

F

V

R

R

F
|
F

A

F

N
|
N

I
x
V

I

Y

G

G

P

P

R

-

S

-

G

-

H

-

G

G

V

E

I

R

T

P

D
|
D

-
x
A

F
x
L

I

V

K

P

K
x
R

L

L

R

C

A

A

N

N

-

L

-

T

P

R

K

N

E

E

L

L

E
x
P

I
x
V

L
x
E

G

G

D

D

G

G

K

E

Q
|
Q

T

R

K
x
R

S

D

Y

F

L

T

E

Y

V

I

T

T

A

D

C

V

V

V

A

-

I

-

S

D

Y

K

V

L

I

V

A

A

H

L

A

A

N

N

D

R

Q

P

V

L

-

P

N

S

T

V

Y

V

N

N

I

F

G

G

S

S

E

G

D

Q

W

S

I

L

D

S

V
|
V

T

N

T

D

I

V

A

I

D

R

I

I

L

L

V

Q

K

A

E

T

A

S

G

P

L

A

A

A

G

E

V

V

S

A

Y

R

K

K

active site: T113 (= T119), C114 (≠ S120), E115 (≠ T121), Y137 (= Y144), K141 (= K148), D176 (= D186)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), I13 (= I12), D32 (= D31), D33 (= D37), R35 (= R39), V36 (≠ F40), P52 (≠ N56), V53 (≠ L57), L70 (≠ I75), A71 (= A76), S72 (≠ A77), K74 (≠ P79), T113 (= T119), K141 (= K148), F165 (= F171), V168 (≠ I174)
binding uridine-diphosphate-n-acetylglucosamine: K74 (≠ P79), T113 (= T119), C114 (≠ S120), E115 (≠ T121), Y137 (= Y144), N167 (= N173), D176 (= D186), A177 (vs. gap), L178 (≠ F187), R181 (≠ K190), P193 (≠ E199), V194 (≠ I200), E195 (≠ L201), Q200 (= Q206), R202 (≠ K208), V236 (= V244)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylglucosamine: 258, 261

2b69A Crystal structure of human udp-glucoronic acid decarboxylase
27% identity, 97% coverage: 2:304/312 of query aligns to 3:301/312 of 2b69A

query
sites
2b69A

R

R

S

I

I

L

V

I

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
x
V

G

G

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active site: T115 (= T119), S116 (= S120), E117 (≠ T121), Y144 (= Y144), K148 (= K148), R185 (vs. gap)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (= F11), V13 (≠ I12), D32 (= D31), N33 (≠ A32), T36 (≠ A35), G37 (= G36), D57 (≠ N56), V58 (≠ L57), L72 (≠ I75), A73 (= A76), S74 (≠ A77), A76 (≠ P79), T91 (≠ N94), T115 (= T119), Y144 (= Y144), K148 (= K148), I171 (≠ F171), N173 (= N173), R185 (vs. gap)
binding uridine-5'-diphosphate: P61 (= P60), L62 (≠ G61), Y63 (≠ W62), P78 (≠ V81), N98 (≠ R101), G101 (≠ A105), L102 (≠ I106), K104 (≠ L108), R105 (≠ H109), Y158 (= Y158), N173 (= N173), R185 (vs. gap), V186 (= V183), N189 (≠ D186), T201 (≠ E199), Y203 (≠ L201), Q208 (= Q206), R210 (≠ K208), I244 (≠ V244), D270 (= D274)

Q8NBZ7 UDP-glucuronic acid decarboxylase 1; UDP-glucuronate decarboxylase 1; UGD; UXS-1; hUXS; hUXS1; EC 4.1.1.35 from Homo sapiens (Human) (see 4 papers)
27% identity, 97% coverage: 2:304/312 of query aligns to 90:388/420 of Q8NBZ7

query
sites
Q8NBZ7

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G98 (= G10) binding NAD(+)
F99 (= F11) binding NAD(+)
V100 (≠ I12) binding NAD(+)
D119 (= D31) binding NAD(+)
N120 (≠ A32) binding NAD(+)
F122 (≠ L34) binding NAD(+)
T123 (≠ A35) binding NAD(+)
G124 (= G36) binding NAD(+)
D144 (≠ N56) binding NAD(+)
V145 (≠ L57) binding NAD(+)
L149 (≠ G61) binding UDP-alpha-D-glucuronate
Y150 (≠ W62) binding UDP-alpha-D-glucuronate
L159 (≠ I75) binding NAD(+)
S161 (≠ A77) binding NAD(+)
K177 (≠ N93) binding UDP-alpha-D-glucuronate
T178 (≠ N94) binding NAD(+)
N185 (≠ R101) binding UDP-alpha-D-glucuronate
G188 (≠ A105) binding UDP-alpha-D-glucuronate
K191 (≠ L108) binding UDP-alpha-D-glucuronate
R192 (≠ H109) binding UDP-alpha-D-glucuronate
A200 (≠ T117) binding NAD(+)
E204 (≠ T121) mutation to A: Reduced UDP-glucuronic acid decarboxylase activity.
Y231 (= Y144) active site, Proton acceptor; binding NAD(+); mutation to F: Abolished UDP-glucuronic acid decarboxylase activity.
K235 (= K148) binding NAD(+)
R236 (≠ L149) mutation to H: Strongly reduced UDP-glucuronic acid decarboxylase activity, caused by a local unfolding of the active site that allows for a rotation of the dimer interface, leading to the formation of a homohexamer.
Y245 (= Y158) binding UDP-alpha-D-glucuronate
Q248 (≠ S161) binding UDP-alpha-D-glucuronate
E249 (≠ F162) binding UDP-alpha-D-glucuronate
T261 (≠ I174) binding NAD(+)
H267 (≠ G180) binding NAD(+)
R272 (vs. gap) binding NAD(+)
R361 (≠ M278) mutation to Q: Strongly reduced UDP-glucuronic acid decarboxylase activity.

Sites not aligning to the query:

1 modified: N-acetylmethionine

6zljA Crystal structure of udp-glucuronic acid 4-epimerase y149f mutant from bacillus cereus in complex with udp-4-deoxy-4-fluoro-glucuronic acid and NAD (see paper)
31% identity, 82% coverage: 1:256/312 of query aligns to 1:262/314 of 6zljA

query
sites
6zljA

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active site: T126 (= T119), S127 (= S120), S128 (≠ T121), F149 (≠ Y144), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D31), H33 (vs. gap), F34 (vs. gap), I35 (vs. gap), K43 (≠ L34), D62 (≠ N56), I63 (≠ L57), L81 (≠ I75), A82 (= A76), A83 (= A77), I124 (≠ T117), T126 (= T119), K153 (= K148), Y176 (≠ F171), T178 (≠ N173), V179 (≠ I174), R185 (≠ G180), M188 (≠ V183)
binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: P85 (= P79), R88 (= R82), T126 (= T119), S127 (= S120), S128 (≠ T121), F149 (≠ Y144), F177 (≠ A172), T178 (≠ N173), R185 (≠ G180), M188 (≠ V183), A189 (≠ I184), R192 (≠ F187), T204 (≠ E199), F206 (≠ L201), Q211 (= Q206), R213 (≠ K208), I250 (≠ V244)

Sites not aligning to the query:

binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: 276

6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
32% identity, 80% coverage: 1:251/312 of query aligns to 1:257/313 of 6bwlA

query
sites
6bwlA

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Y

K

D

I

V

P

-

E

K

I

M

V

V

F

F

T
x
M

S

S

T
|
T

S
x
C

T
x
M

V

V

Y

Y

G

D

E

K

A

A

T

T

V

N

I

I

P

Q

T

G

P

I

E

S

T

E

Y

L

T

D

P

P

M

I

E

K

P

P

V

A

S

S

V

P

Y
|
Y

G

A

A

G

A

S

K
|
K

L

I

A

A

C

A

E

E

A

N

L

M

I

V

S

L

A

S

Y

Y

C

Y

H

Y

S

A

F

Y

G

K

V

L

R

P

G

V

Y

V

I

V

F

I

R

R

F

P

A

F

N
|
N

I
x
T

I

Y

G

G

P

P

-

F

-

Q

R

K

S

T

G

G

-

G

-

E

H

G

G
|
G

V
|
V

I

V

T

A

D

I

F

F

I

I

-

N

K

N

K

K

L

L

R

D

A

N

N

V

P

P

K

-

E

-

L

L

E
x
N

I
|
I

L
x
Y

G

G

D

D

G

G

K

K

Q
|
Q

T

T

K
x
R

S

D

Y

L

L

L

E

Y

V

V

T

E

A

D

C

C

-

A

-

D

-

F

V

V

A

V

A

A

I

A

S

G

Y

Y

V

S

I

-

A

A

H

K

A

A

N

N

D

G

Q

H

V

I

N

-

T

-

Y

I

N

N

I

A

G

G

S

T

E

G

D

Q

W

D

I

I

D

S

V
x
I

T

N

T

K

I

L

A

A

D

E

I

L

L

I

active site: T122 (= T119), C123 (≠ S120), M124 (≠ T121), Y147 (= Y144), K151 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), N32 (≠ A32), L33 (= L33), N35 (vs. gap), S36 (vs. gap), D57 (≠ A49), I58 (≠ V50), L79 (≠ I75), A80 (= A76), A81 (= A77), I83 (≠ P79), M120 (≠ T117), K151 (= K148), N176 (= N173), T177 (≠ I174)
binding uridine-5'-diphosphate: N176 (= N173), G189 (= G182), V190 (= V183), N205 (≠ E199), I206 (= I200), Y207 (≠ L201), Q212 (= Q206), R214 (≠ K208), I250 (≠ V244)

Sites not aligning to the query:

binding uridine-5'-diphosphate: 275

6kvcA Moee5 in complex with udp-glucose and NAD (see paper)
30% identity, 95% coverage: 4:300/312 of query aligns to 4:287/299 of 6kvcA

query
sites
6kvcA

I

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

I

L

V

V

D

T

H

E

L

L

V

R

A

N

Q

S

G

G

D

R

E

N

V

V

V

A

I

V

D
|
D

A

-

L

-

A

-

G

-

D

-

L

-

R

-

F

-

I

-

S
x
R

R
|
R

H

P

L

L

D

P

S

T

G

G

A

S

V

L

K

R

F

E

I

I

R

R

A

G

N
x
D

L
|
L

L

N

E

S

P

L

G

N

W

L

Q

V

D

D

V

C

C

L

A

K

G

N

A

I

D

S

R

T

V

V

W

F

H

H

I
x
L

A
|
A

D

L

P
|
P

D

G

V

V

R
|
R

E

P

S

S

A

W

V

T

T

Q

P

F

D

P

A

E

Q

Y

L

L

N

R

N

C

T

N

I

V

I

L

A

A

T

T

H

Q

R

R

V

L

L

M

E

E

A

A

I

C

R

V

L

Q

H

A

K

G

I

V

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E

E

R

I

V

V

V

F

V

T
x
A

S

S

T
x
S

S
|
S

T
x
S

V

V

Y

Y

G

G

E

G

A

A

T

D

V

G

I

V

P

M

T

S

P

E

E

D

T

D

Y

L

T

P

P

-

M

-

E

R

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P

V

L

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S

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Y
|
Y

G

G

A

V

A

T

K
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K

L

L

A

A

C

A

E

E

A

R

L

L

I

A

S

L

A

A

Y

-

C

-

H

-

S

-

F

F

G

A

V

A

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R

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G

Y

D

-

A

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L

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G

I

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|
F

A

F

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x
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x
V

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Y

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-

G

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x
R

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x
M

V
x
F

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x
R

F

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-

A

K

T

L

L

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R

A

G

N

E

P

P

K

-

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-

L

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E

I
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I

L
x
Y

G

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D

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Q

T

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K
x
R

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D

Y

F

L

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H

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A

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C

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A

R

A

A

I

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M

Y

L

V

T

I

A

A

S

H

V

A

R

N

D

D

R

Q

G

V

S

N

A

T

V

Y

L

N

N

I

I

G

G

S

T

E

G

D

S

W

A

I

V

D

S

V

V

T

N

T

E

I

V

A

V

D

S

I

M

L

T

V

A

K

E

E

L

A

T

G

G

L

L

A

R

G

P

V

C

S

T

Y

A

K

Y

Y

-

T

-

G

G

G

S

S

A

R

R

G

-

W

-

V

I

G

G

D
|
D

V

V

P

R

K

S

M

T

Q

T

L

A

S

D

V

V

E

R

K

Q

L

A

K

Q

S

S

-

V

L

L

G

G

Y

F

T

T

P

A

T

R

I

T

G

G

S

L

H

R

E

E

S

G

V

L

active site: S109 (≠ T119), S110 (= S120), S111 (≠ T121), Y132 (= Y144), K136 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), R32 (≠ S42), R33 (= R43), D46 (≠ N56), L47 (= L57), L65 (≠ I75), A66 (= A76), A67 (= A77), P69 (= P79), A107 (≠ T117), S109 (≠ T119), K136 (= K148), F161 (= F171), T163 (≠ N173), V164 (≠ I174), R170 (≠ S179), M173 (≠ G182)
binding uridine-5'-diphosphate-glucose: P69 (= P79), R72 (= R82), S109 (≠ T119), S110 (= S120), Y132 (= Y144), T163 (≠ N173), M173 (≠ G182), F174 (≠ V183), R177 (≠ D186), E189 (= E199), I190 (= I200), Y191 (≠ L201), Q196 (= Q206), R198 (≠ K208), D260 (= D274)

6kv9A Moee5 in complex with udp-glucuronic acid and NAD (see paper)
31% identity, 95% coverage: 4:300/312 of query aligns to 4:288/299 of 6kv9A

query
sites
6kv9A

I

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

I

L

V

V

D

T

H

E

L

L

V

R

A

N

Q

S

G

G

D

R

E

N

V

V

V

A

I

V

D
|
D

A

-

L

-

A

-

G

-

D

-

L

-

R

-

F

-

I

-

S
x
R

R
|
R

H

P

L

L

-

P

D

D

S

T

G

G

A

S

V

L

K

R

F

E

I

I

R

R

A

G

N
x
D

L
|
L

L

N

E

S

P

L

G

N

W

L

Q

V

D

D

V

C

C

L

A

K

G

N

A

I

D

S

R

T

V

V

W

F

H

H

I
x
L

A
|
A

D

L

P
|
P

D

G

V

V

R
|
R

E

P

S

S

A

W

V

T

T

Q

P

F

D

P

A

E

Q

Y

L

L

N

R

N
x
C

T

N

I

V

I

L

A

A

T

T

H

Q

R

R

V

L

L

M

E

E

A

A

I

C

R

V

L

Q

H

A

K

G

I

V

P

E

E

R

I

V

V

V

F

V

T
x
A

S
|
S

T
x
S

S
|
S

T
x
S

V

V

Y

Y

G

G

E

G

A

A

T

D

V

G

I

V

P

M

T

S

P

E

E

D

T

D

Y

L

T

P

P

-

M

-

E

R

P

P

V

L

S

S

V

P

Y
|
Y

G

G

A

V

A

T

K
|
K

L

L

A

A

C

A

E

E

A

R

L

L

I

A

S

L

A

A

Y

-

C

-

H

-

S

-

F

F

G

A

V

A

R

R

G

G

Y

D

-

A

-

E

-

L

-

S

-

V

-

G

I

A

F

L

R

R

F
|
F

A

F

N
x
T

I
x
V

I

Y

G

G

P

P

-

G

R

Q

S
x
R

G

P

H

D

G
x
M

V
x
F

I

I

T

S

D
x
R

F

L

I

I

K

R

-

A

K

T

L

L

R

R

A

G

N

E

P

P

K

-

E

-

L

V

E
|
E

I
|
I

L
x
Y

G

G

D

D

G

G

K

T

Q
|
Q

T

L

K
x
R

S

D

Y

F

L

T

E

H

V

V

T

S

A

D

C

V

V

V

A

R

A

A

I

L

S

M

Y

L

V

T

I

A

A

S

H

V

A

R

N

D

D

R

Q

G

V

S

N

A

T

V

Y

L

N

N

I

I

G

G

S

T

E

G

D

S

W

A

I

V

D

S

V
|
V

T

N

T

E

I

V

A

V

D

S

I

M

L

T

V

A

K

E

E

L

A

T

G

G

L

L

A

R

G

P

V

C

S

T

Y

A

K

Y

Y

-

T

-

G

G

G

S

S

A

R

R

G

-

W

-

V

I

G

G

D
|
D

V

V

P

R

K

S

M

T

Q

T

L

A

S

D

V

V

E

R

K

Q

L

A

K

Q

S

S

-

V

L

L

G

G

Y

F

T

T

P

A

T

R

I

T

G

G

S

L

H

R

E

E

S

G

V

L

active site: S110 (≠ T119), S111 (= S120), S112 (≠ T121), Y133 (= Y144), K137 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (= I12), D31 (= D31), R32 (≠ S42), R33 (= R43), D47 (≠ N56), L48 (= L57), L66 (≠ I75), A67 (= A76), A68 (= A77), P70 (= P79), C85 (≠ N94), A108 (≠ T117), S109 (= S118), K137 (= K148), F162 (= F171), T164 (≠ N173), V165 (≠ I174), R171 (≠ S179), M174 (≠ G182)
binding uridine-5'-diphosphate-glucuronic acid: P70 (= P79), R73 (= R82), S110 (≠ T119), S111 (= S120), Y133 (= Y144), T164 (≠ N173), R171 (≠ S179), M174 (≠ G182), F175 (≠ V183), R178 (≠ D186), E190 (= E199), I191 (= I200), Y192 (≠ L201), Q197 (= Q206), R199 (≠ K208), V235 (= V244), D261 (= D274)

Query Sequence

>WP_109967995.1 NCBI__GCF_003173355.1:WP_109967995.1
MRSIVTGGAGFIGSHIVDHLVAQGDEVVVIDALLAGDLRFISRHLDSGAVKFIRANLLEP
GWQDVCAGADRVWHIAADPDVRESAVTPDAQLNNTIIATHRVLEAIRLHKIPEIVFTSTS
TVYGEATVIPTPETYTPMEPVSVYGAAKLACEALISAYCHSFGVRGYIFRFANIIGPRSG
HGVITDFIKKLRANPKELEILGDGKQTKSYLEVTACVAAISYVIAHANDQVNTYNIGSED
WIDVTTIADILVKEAGLAGVSYKYTGGSRGWVGDVPKMQLSVEKLKSLGYTPTIGSHESV
RLAVRAALSGQE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory