SitesBLAST
Comparing WP_109968238.1 NCBI__GCF_003173355.1:WP_109968238.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
49% identity, 97% coverage: 8:489/496 of query aligns to 7:488/502 of P07117
- R257 (= R263) mutation to C: Sodium-independent binding affinity for proline.
- C281 (≠ S287) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- C344 (≠ S345) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
- C349 (≠ T350) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- R376 (= R377) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.
Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
28% identity, 69% coverage: 31:370/496 of query aligns to 42:382/643 of Q92911
- A102 (≠ W91) natural variant: A -> P
- H226 (≠ A222) mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
- D237 (≠ F231) mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
- Y242 (≠ G236) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
- T243 (= T237) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
Sites not aligning to the query:
- 471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
- 525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
- 536 T → Q: requires 2 nucleotide substitutions
- 556 S → Q: requires 2 nucleotide substitutions
Q9GZV3 High affinity choline transporter 1; hCHT1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Homo sapiens (Human) (see 5 papers)
24% identity, 87% coverage: 7:438/496 of query aligns to 7:442/580 of Q9GZV3
- D48 (≠ G42) to G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039768
- G65 (= G59) to E: in CMS20; loss of choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039765
- I89 (≠ L81) to V: 40% reduction in choline transmembrane transporter activity; found in 0.06 of Ashkenazi Jews; dbSNP:rs1013940; mutation to A: Decreased choline transmembrane transporter activity, only 20% of wild-type choline uptake activity.
- P105 (≠ R97) to S: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039766
- Y111 (≠ S108) to H: in CMS20; no effect on localization at plasma membrane
- Y175 (= Y175) to C: in CMS20; uncertain significance; dbSNP:rs1331713195
- I291 (≠ V297) to T: in CMS20; uncertain significance; dbSNP:rs375397889
- V344 (≠ I339) to L: in CMS20; uncertain significance
- R361 (≠ E356) to Q: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs147656110
- F418 (≠ G414) to V: in CMS20; uncertain significance
Sites not aligning to the query:
- 446 R → G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane
- 451 E→Q: Decreased choline transmembrane transporter activity, only 5% of wild-type choline uptake activity.
- 527:532 Dileucine-like motif
- 530 I→A: No change in protein internalization. No change in choline transmembrane transporter activity.
- 531 L→A: Loss of protein internalization to vesicular structures in neurons. Increased choline transmembrane transporter activity.
- 531:532 LV→AA: Decreased protein internalization; when associated with V-538. Increased choline transmembrane transporter activity; when associated with V-538.
- 532 V→A: Decreased protein internalization. Increased choline transmembrane transporter activity.
- 538 K→V: Decreased protein internalization; when associated with 531-L-V-532. Increased choline transmembrane transporter activity; when associated with 531-L-V-532.
8j74A Human high-affinity choline transporter cht1 in the hc-3-bound outward-facing open conformation, dimeric state (see paper)
24% identity, 87% coverage: 7:438/496 of query aligns to 5:440/515 of 8j74A
- binding Lauryl Maltose Neopentyl Glycol: F203 (≠ I204), S206 (≠ G211), H207 (≠ G212), I213 (≠ D218), G214 (≠ S219), F215 (≠ I220), H219 (≠ N224), C326 (≠ F323), P327 (≠ F324)
- binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): W60 (≠ D56), Y89 (≠ L83), W139 (≠ Y141), L245 (≠ W250), W252 (≠ H259), W404 (≠ V402), Y405 (≠ S403), S408 (≠ W406)
8zqpA High affinity choline transporter 1
24% identity, 87% coverage: 7:438/496 of query aligns to 4:439/470 of 8zqpA
9bimA Cryo-em structure of human cht1 in the hc-3 bound outward-facing state
25% identity, 87% coverage: 7:438/496 of query aligns to 6:439/516 of 9bimA
- binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): W59 (≠ D56), Y77 (≠ M74), Y88 (≠ L83), W138 (≠ Y141), W251 (≠ Y254), Y404 (≠ S403), S407 (≠ W406)
Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
23% identity, 87% coverage: 9:438/496 of query aligns to 29:492/659 of Q9NY91
- E457 (vs. gap) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.
8j77A Human high-affinity choline transporter cht1 in the choline-bound inward-facing occluded conformation (see paper)
24% identity, 80% coverage: 41:438/496 of query aligns to 27:418/493 of 8j77A
P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
23% identity, 87% coverage: 9:438/496 of query aligns to 29:492/662 of P11170
- C255 (vs. gap) modified: Disulfide link with 608
- Q457 (≠ Y399) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
- T460 (≠ V402) mutation to W: Decreased affinity for glucose and phlorizin.
Sites not aligning to the query:
- 608 modified: Disulfide link with 255
Q9Y289 Sodium-dependent multivitamin transporter; Na(+)-dependent multivitamin transporter; hSMVT; Solute carrier family 5 member 6 from Homo sapiens (Human) (see 10 papers)
25% identity, 82% coverage: 31:438/496 of query aligns to 54:463/635 of Q9Y289