SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_109968633.1 NCBI__GCF_003173355.1:WP_109968633.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
58% identity, 98% coverage: 7:253/253 of query aligns to 11:257/257 of P0AAH0

query
sites
P0AAH0

I

I

N

Q

V

V

R

R

N

N

L

L

N

N

L

F

W

Y

Y

Y

G

G

E

K

T

F

Q

H

A

A

L

L

R

K

N

N

I

I

S

N

M

L

P

D

I

I

A

A

H

K

Q

N

K

Q

V

V

T

T

S

A

L

F

I

I

G

G

P

P

S

S

G

G

C

C

G
|
G

K
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K

S

S

T

T

L

L

I

L

R

R

C

T

F

F

N

N

R

K

M

M

N

F

D

E

L

L

V

Y

S

P

G

E

C

Q

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R

I

A

E

E

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E

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D

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A

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D

A

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D

L

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M

A

Q

K

I

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G

G

M

M

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F

F

Q

Q

K

K

P

P

N

T

P

P

F

F

P

P

K

M

T

S

I

I

Y

Y

E

D

N

N

I

I

A

A

Y

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G

P

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E

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D

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R

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S

A

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A

A

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W

E

N

E

E

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T

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K

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H

D

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Y

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A

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A

A

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C

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S

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D

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H

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T

A

A

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M

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Y

L

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E

L

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G

G

G48 (= G44) mutation to I: Loss of phosphate transport.
K49 (= K45) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
37% identity, 96% coverage: 6:248/253 of query aligns to 2:236/241 of 4u00A

query
sites
4u00A

I

I

N

R

V

I

R

R

N

N

L

L

N

H

L

K

W

W

Y
x
F

G

G

E

P

T

L

Q

H

A
x
V

L

L

R

K

N

G

I

I

S

H

M

L

P

E

I

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A

A

H

P

Q

G

K

E

V

K

T

L

S

V

L

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

R

R

C

T

F

I

N

N

R

R

M

L

N

E

D

D

L

F

V

Q

S

-

G

-

C

-

R

-

I

-

E

E

G

G

S

E

L

V

T

V

F

V

H

D

G

G

R

L

D

S

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G

D

S

D

G

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A

A

D

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A

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E

I

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R

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E

I

V

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G

M

M

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V

F

F

Q

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Q

P

F

N

N

P

L

F

F

P

P

K

H

-

M

T

T

I

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Y

L

E

E

N

N

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Y

L

G

A

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P

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M

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E

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R

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A

G

L

I

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L

E

D

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V

A

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R

T

K

R

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L

-

H

-

D

-

S

Y

A

P

L

A

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Q

L

L

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S

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G

Q

Q

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R

L

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C

A

I

I

A

A

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A

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A

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D

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Y

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M

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T

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H

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E

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A

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D

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A

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F

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M

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G

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P

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K

K

E

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E

L

R

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R

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S

Y

F

I

L

binding adenosine-5'-diphosphate: F12 (≠ Y16), V17 (≠ A21), S37 (= S41), G38 (= G42), S39 (≠ C43), G40 (= G44), K41 (= K45), S42 (= S46), T43 (= T47)

3c4jA Abc protein artp in complex with atp-gamma-s
35% identity, 98% coverage: 6:252/253 of query aligns to 3:242/242 of 3c4jA

query
sites
3c4jA

I

M

I

I

N

D

V

V

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N

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L

L

N

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L

K

W

S

Y
x
F

G

G

E

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L

Q

E

A
x
V

L

L

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K

N

G

I

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N

M

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P

H

I

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A

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H

E

Q

G

K

E

V

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T

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S

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L

V

I

I

G

G

P

P

S
|
S

G
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G

C
x
S

G
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G

K
|
K

S
|
S

T
|
T

L

F

I

L

R

R

C

C

F

L

N

N

R

L

M

L

N

E

D

D

L

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V

-

S

-

G

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C

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R

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I

D

E

E

G

G

S

E

L

I

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I

F

I

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D

G

G

R

I

D

N

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Y

K

G

A

S

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G

D

A

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D

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A

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M

E

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E

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G

M

M

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F

F

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R

P

F

N

N

P

L

F

F

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K

H

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M

T

T

I

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Y

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E

N

N

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A

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A

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K

A

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G

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E

-

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K

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A

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L

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C

A

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I

A

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A

K

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D

E

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Y

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M

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H

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A

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D

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Y

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G

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D

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R

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H

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T

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F

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G

K

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F

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y16), V18 (≠ A21), S38 (= S41), G39 (= G42), S40 (≠ C43), G41 (= G44), K42 (= K45), S43 (= S46), T44 (= T47)

3c41J Abc protein artp in complex with amp-pnp/mg2+
35% identity, 98% coverage: 6:252/253 of query aligns to 3:242/242 of 3c41J

query
sites
3c41J

I

M

I

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N

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W

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Y
x
F

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x
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G

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G

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K

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T

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C

F

L

N

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D

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E

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G

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V

F

F

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Q

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R

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F

N

N

P

L

F

F

P

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K

H

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M

T

T

I

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Y

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E

N

N

N

I

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A

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D

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K

A

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E

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V

-

K

K

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A

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A

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A

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G

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A

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Q

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C

A

I

I

A

A

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L

A

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N

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E

K

I

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F

D
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D

E

E

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C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

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A

G

K

E

I

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L

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A

K

N

D

E

-

G

Y

M

C

T

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M

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I

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T

H

H

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M

S

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Q

F

A

A

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G

D

D

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A

L

F

F

M

M

Y

D

L

G

G

G

D

Y

L

I

I

I

E

E

F

E

G

G

E

K

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P

R

E

Q

D

I

L

F

F

E

D

N

R

P

P

K

Q

K

H

E

E

L

R

T

T

E

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y16), V18 (≠ A21), S38 (= S41), G39 (= G42), G41 (= G44), K42 (= K45), S43 (= S46), T44 (= T47)
binding magnesium ion: S43 (= S46), D163 (= D174)

2olkA Abc protein artp in complex with adp-beta-s
35% identity, 98% coverage: 6:252/253 of query aligns to 3:242/242 of 2olkA

query
sites
2olkA

I

M

I

I

N

D

V

V

R

H

N

Q

L

L

N

K

L

K

W

S

Y
x
F

G

G

E

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L

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A
x
V

L

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R

K

N

G

I

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P

H

I

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H

E

Q

G

K

E

V

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S

V

L

V

I

I

G

G

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P

S
|
S

G
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G

C
x
S

G
|
G

K
|
K

S
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S

T
|
T

L

F

I

L

R

R

C

C

F

L

N

N

R

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M

L

N

E

D

D

L

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S

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I

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D

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G

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I

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N

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K

G

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K

G

D

A

T

D

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A

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N

D

K

I

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M

E

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E

I

V

G

G

M

M

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V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

T

T

I

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Y

L

E

N

N

N

I

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A

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E

K

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S

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D

K

K

A

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G

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W

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E

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-

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K

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K

L

A

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H

D

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Y

A

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G

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A

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Q

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C

A

I

I

A

A

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A

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L

A

A

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M

N

E

P

P

E

K

I

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F

D

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S

A

A

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D

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P

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A

G

K

E

I

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E

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K

E

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L

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K

A

K

N

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E

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M

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T

H

H

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M

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F

A

A

A

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D

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F

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M

Y

D

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Y

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G

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T

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K

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E

E

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K

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F

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K

R

V

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y16), V18 (≠ A21), S38 (= S41), G39 (= G42), S40 (≠ C43), G41 (= G44), K42 (= K45), S43 (= S46), T44 (= T47)

2oljA Abc protein artp in complex with adp/mg2+
35% identity, 98% coverage: 6:252/253 of query aligns to 3:242/242 of 2oljA

query
sites
2oljA

I

M

I

I

N

D

V

V

R

H

N

Q

L

L

N

K

L

K

W

S

Y
x
F

G

G

E

S

T

L

Q

E

A
x
V

L

L

R

K

N

G

I

I

S

N

M

V

P

H

I

I

A

R

H

E

Q

G

K

E

V

V

T

V

S

V

L

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

R

R

C

C

F

L

N

N

R

L

M

L

N

E

D

D

L

F

V

-

S

-

G

-

C

-

R

-

I

D

E

E

G

G

S

E

L

I

T

I

F

I

H

D

G

G

R

I

D

N

I

L

Y

K

G

A

S

K

G

D

A

T

D

N

A

L

V

N

D

K

I

V

R

R

M

E

Q

E

I

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

T

T

I

V

Y

L

E

N

N

N

I

I

A

T

Y

L

G

A

P

P

R

M

I

K

H

V

G

R

I

K

R

W

D

P

R

R

A

E

E

K

L

A

D

E

R

A

I

K

V

A

E

M

S

E

S

L

L

L

K

D

K

K

A

V

A

G

L

L

W

-

E

-

V

-

K

K

T

D

R

K

L

A

H

H

D

A

S

Y

A

P

L

D

G

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

T

A

L

L

A

A

V

M

N

E

P

P

E

K

I

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

K

E

I

V

E

L

D

S

L

V

I

M

D

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

C

T

V

M

V

V

I

V

V

V

T

T

H

H

S

E

M

M

S

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

F

R

T

V

A

L

F

F

M

M

Y

D

L

G

G

G

D

Y

L

I

I

I

E

E

F

E

G

G

E

K

T

P

R

E

Q

D

I

L

F

F

E

D

N

R

P

P

K

Q

K

H

E

E

L

R

T

T

E

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding adenosine-5'-diphosphate: F13 (≠ Y16), V18 (≠ A21), S38 (= S41), G39 (= G42), S40 (≠ C43), G41 (= G44), K42 (= K45), S43 (= S46), T44 (= T47)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
34% identity, 92% coverage: 16:249/253 of query aligns to 11:237/240 of 4ymuJ

query
sites
4ymuJ

Y
x
F

G

G

E

S

T

L

Q

E

A
x
V

L

L

R

K

N

G

I

V

S

T

M

L

P

K

I

V

A

N

H

K

Q

G

K

E

V

V

T

V

S

V

L

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

R

R

C

C

F

I

N

N

R

L

M

L

N

E

D

E

L

-

V

-

S

-

G

-

C

-

R

P

I

T

E

K

G

G

S

E

L

V

T

F

F

I

H

D

G

G

R

V

D

K

I

I

Y

N

G

N

S

G

G

K

A

V

D

N

A

I

V

N

D

K

I

V

R

R

M

Q

Q

K

I

V

G

G

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

F

P

P

K

H

-

L

T

T

I

A

Y

I

E

E

N

N

I

I

A

T

Y

L

G

A

P

P

R

V

I

K

H

V

G

K

I

K

R

M

D

N

R

K

A

K

E

E

L

A

D

E

R

E

I

L

V

A

E

V

S

D

S

L

L

L

K

A

K

K

A

V

A

G

L

L

W

L

E

D

E

-

V

-

K

-

T

-

R

K

L

K

H

D

D

Q

S

Y

A

P

L

I

G

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

C

A

I

I

A

A

R

R

T

A

L

L

A

A

V

M

N

Q

P

P

E

E

I

V

I

M

L

L

M

F

D

D

E
|
E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

K

K

E

I

V

E

L

D

N

L

V

I

M

D

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

C

T

V

M

V

V

I

V

V

V

T

T

H
|
H

S

E

M

M

S

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

F

R

T

V

A

I

F

F

M

M

Y

D

L

D

G

G

D

V

L

I

I

V

E

E

F

E

G

G

E

T

T

P

R

E

Q

E

I

I

F

F

E

Y

N

R

P

A

K

K

K

N

E

E

L

R

T

T

E

R

N

E

Y

F

I

L

T

S

binding adenosine-5'-triphosphate: F11 (≠ Y16), V16 (≠ A21), S36 (= S41), G37 (= G42), S38 (≠ C43), G39 (= G44), K40 (= K45), S41 (= S46), T42 (= T47), E162 (= E175), H194 (= H206)
binding magnesium ion: S41 (= S46), E162 (= E175)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 91% coverage: 20:248/253 of query aligns to 19:241/343 of P30750

query
sites
P30750

Q

Q

A

A

L

L

R

N

N

N

I

V

S

S

M

L

P

H

I

V

A

P

H

A

Q

G

K

Q

V

I

T

Y

S

G

L

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

R

R

C

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

V

-

S

-

G

-

C

-

R

P

I

T

E

E

G

G

S

S

L

V

T

L

F

V

H

D

G

G

R

Q

D

E

I

L

Y

T

G

T

-

L

S

S

G

E

A

S

D

E

A

L

V

T

D

K

I

A

R

R

M

R

Q

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

T

T

I

V

Y

F

E

G

N

N

I

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

I

T

R

-

D

P

R

K

A

D

E

E

L

V

D

K

R

R

I

R

V

V

E

T

S

E

S

L

L

L

K

S

K

L

A

V

A

G

L

L

W

G

E

D

E

K

V

-

K

-

T

-

R

-

L

-

H

H

D

D

S

S

-

Y

A

P

L

S

G

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

T

A

L

L

A

A

V

S

N

N

P

P

E

K

I

V

I

L

L

L

M

C

D

D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

D

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

C

T

V

I

V

L

I

L

V

I

T

T

H

H

S

E

M

M

S

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

F

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

D

E

L

L

I

I

E

E

F

Q

G

D

E

T

T

V

R

S

Q

E

I

V

F

F

E

S

N

H

P

P

K

K

K

T

E

P

L

L

T

A

E

Q

N

K

Y

F

I

I

40:46 (vs. 41:47, 86% identical) binding ATP
E166 (= E175) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
35% identity, 91% coverage: 20:248/253 of query aligns to 20:242/344 of 3tuzC

query
sites
3tuzC

Q

Q

A

A

L

L

R

N

N

N

I

V

S

S

M

L

P

H

I

V

A

P

H

A

Q

G

K

Q

V

I

T

Y

S

G

L

V

I

I

G

G

P

A

S

S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

R

R

C

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

V

-

S

-

G

-

C

-

R

P

I

T

E

E

G

G

S

S

L

V

T

L

F

V

H

D

G

G

R

Q

D

E

I

L

Y

T

G

T

-

L

S

S

G

E

A

S

D

E

A

L

V

T

D

K

I

A

R

R

M

R

Q

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

T

T

I

V

Y

F

E

G

N

N

I

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

I

T

R

-

D

P

R

K

A

D

E

E

L

V

D

K

R

R

I

R

V

V

E

T

S

E

S

L

L

L

K

S

K

L

A

V

A

G

L

L

W

G

E

D

E

K

V

-

K

-

T

-

R

-

L

-

H

H

D

D

S

S

-

Y

A

P

L

S

G

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

T

A

L

L

A

A

V

S

N

N

P

P

E

K

I

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

D

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

C

T

V

I

V

L

I

L

V

I

T

T

H

H

S

E

M

M

S

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

F

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

D

E

L

L

I

I

E

E

F

Q

G

D

E

T

T

V

R

S

Q

E

I

V

F

F

E

S

N

H

P

P

K

K

K

T

E

P

L

L

T

A

E

Q

N

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G42), G44 (= G44), K45 (= K45), S46 (= S46), T47 (= T47)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
35% identity, 91% coverage: 20:248/253 of query aligns to 20:242/344 of 3tuiC

query
sites
3tuiC

Q

Q

A

A

L

L

R

N

N

N

I

V

S

S

M

L

P

H

I

V

A

P

H

A

Q

G

K

Q

V

I

T

Y

S

G

L

V

I

I

G

G

P

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

R

R

C

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

V

-

S

-

G

-

C

-

R

P

I

T

E

E

G

G

S

S

L

V

T

L

F

V

H

D

G

G

R

Q

D

E

I

L

Y

T

G

T

-

L

S

S

G

E

A

S

D

E

A

L

V

T

D

K

I

A

R

R

M

R

Q

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

T

T

I

V

Y

F

E

G

N

N

I

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

I

T

R

-

D

P

R

K

A

D

E

E

L

V

D

K

R

R

I

R

V

V

E

T

S

E

S

L

L

L

K

S

K

L

A

V

A

G

L

L

W

G

E

D

E

K

V

-

K

-

T

-

R

-

L

-

H

H

D

D

S

S

-

Y

A

P

L

S

G

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

T

A

L

L

A

A

V

S

N

N

P

P

E

K

I

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

D

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

C

T

V

I

V

L

I

L

V

I

T

T

H

H

S

E

M

M

S

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

F

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

D

E

L

L

I

I

E

E

F

Q

G

D

E

T

T

V

R

S

Q

E

I

V

F

F

E

S

N

H

P

P

K

K

K

T

E

P

L

L

T

A

E

Q

N

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G42), A43 (≠ C43), G44 (= G44), K45 (= K45), S46 (= S46), T47 (= T47)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
35% identity, 91% coverage: 20:248/253 of query aligns to 20:242/344 of 6cvlD

query
sites
6cvlD

Q

Q

A

A

L

L

R

N

N

N

I

V

S

S

M

L

P

H

I

V

A

P

H

A

Q

G

K

Q

V

I

T

Y

S

G

L

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

R

R

C

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

V

-

S

-

G

-

C

-

R

P

I

T

E

E

G

G

S

S

L

V

T

L

F

V

H

D

G

G

R

Q

D

E

I

L

Y

T

G

T

-

L

S

S

G

E

A

S

D

E

A

L

V

T

D

K

I

A

R

R

M

R

Q

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

T

T

I

V

Y

F

E

G

N

N

I

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

I

T

R

-

D

P

R

K

A

D

E

E

L

V

D

K

R

R

I

R

V

V

E

T

S

E

S

L

L

L

K

S

K

L

A

V

A

G

L

L

W

G

E

D

E

K

V

-

K

-

T

-

R

-

L

-

H

H

D

D

S

S

-

Y

A

P

L

S

G
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

T

A

L

L

A

A

V

S

N

N

P

P

E

K

I

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

D

E

L

L

I

L

D

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

C

T

V

I

V

L

I

L

V

I

T

T

H
|
H

S

E

M

M

S

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

F

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

D

E

L

L

I

I

E

E

F

Q

G

D

E

T

T

V

R

S

Q

E

I

V

F

F

E

S

N

H

P

P

K

K

K

T

E

P

L

L

T

A

E

Q

N

K

Y

F

I

I

binding phosphothiophosphoric acid-adenylate ester: S41 (= S41), G42 (= G42), A43 (≠ C43), G44 (= G44), K45 (= K45), S46 (= S46), T47 (= T47), N141 (≠ G149), S143 (= S151), Q146 (= Q154), H200 (= H206)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14, 19

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
32% identity, 92% coverage: 17:248/253 of query aligns to 37:263/382 of 7ahhC

query
sites
7ahhC

G

G

E

A

T
|
T

Q
x
V

A
x
G

L

V

R

Y

N

D

I

T

S

N

M

F

P

E

I

I

A

N

H

E

Q

G

K

E

V

I

T

F

S

V

L

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

L

L

I

L

R

R

C

L

F

L

N

N

R

R

M

L

N

I

D

E

L

P

V

T

S

S

G

G

C

-

R

-

I

-

E

-

G

-

S

K

L

I

T

F

F

I

H

D

G

N

R

Q

D

D

I

V

Y

A

G

T

-

L

S

N

G

K

A

E

D

D

A

L

V

L

D

Q

I

V

R

R

M

R

Q

K

-

T

I

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

T

T

I

I

Y

L

E

E

N

N

I

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

I

V

R

-

D

P

R

K

A

E

E

E

L

R

D

R

R

K

I

R

V

A

E

E

S

K

S

A

L

L

K

D

K

N

A

A

A

N

L

L

W

L

E

D

E

-

V

-

K

-

T

-

R

-

L

F

H

K

D

D

S

Q

-

Y

A

P

L

K

G

Q

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

T

A

L

L

A

A

V

N

N

D

P

P

E

E

I

I

I

L

L

L

M

M

D
|
D

E
|
E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

K

E

I

M

E

Q

D

D

L

E

I

L

D

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

C

T

V

I

I

F

V

V

T

S

H

H

S

D

M

L

S

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

F

R

T

I

A

A

F

I

M

M

Y

K

L

D

G

G

D

K

L

I

I

M

E

Q

F

I

G

G

E

T

T

G

R

E

Q

E

I

I

F

L

E

T

N

N

P

P

K

A

K

N

E

D

L

Y

T

V

E

K

N

T

Y

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: T39 (= T19), V40 (≠ Q20), G41 (≠ A21), G62 (= G42), G64 (= G44), K65 (= K45), D187 (= D174), E188 (= E175)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
32% identity, 92% coverage: 17:248/253 of query aligns to 37:263/382 of 7aheC

query
sites
7aheC

G

G

E

A

T

T

Q

V

A

G

L

V

R

Y

N

D

I

T

S

N

M

F

P

E

I

I

A

N

H

E

Q

G

K

E

V

I

T

F

S

V

L

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

L

L

I

L

R

R

C

L

F

L

N

N

R

R

M

L

N

I

D

E

L

P

V

T

S

S

G

G

C

-

R

-

I

-

E

-

G

-

S

K

L

I

T

F

F

I

H

D

G

N

R

Q

D

D

I

V

Y

A

G

T

-

L

S

N

G

K

A

E

D

D

A

L

V

L

D

Q

I

V

R

R

M

R

Q

K

-

T

I

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

T

T

I

I

Y

L

E

E

N

N

I

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

I

V

R

-

D

P

R

K

A

E

E

E

L

R

D

R

R

K

I

R

V

A

E

E

S

K

S

A

L

L

K

D

K

N

A

A

A

N

L

L

W

L

E

D

E

-

V

-

K

-

T

-

R

-

L

F

H

K

D

D

S

Q

-

Y

A

P

L

K

G

Q

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

T

A

L

L

A

A

V

N

N

D

P

P

E

E

I

I

I

L

L

L

M

M

D

D

E

E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

K

E

I

M

E

Q

D

D

L

E

I

L

D

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

C

T

V

I

I

F

V

V

T

S

H

H

S

D

M

L

S

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

F

R

T

I

A

A

F

I

M

M

Y

K

L

D

G

G

D

K

L

I

I

M

E

Q

F

I

G

G

E

T

T

G

R

E

Q

E

I

I

F

L

E

T

N

N

P

P

K

A

K

N

E

D

L

Y

T

V

E

K

N

T

Y

F

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
31% identity, 92% coverage: 7:239/253 of query aligns to 339:569/571 of 4ayxA

query
sites
4ayxA

I

L

N

E

V

F

R

K

N

N

L

V

N

H

L

F

W

A

Y
|
Y

G

P

-

A

-

R

-

P

E

E

T

V

Q

P

A

I

L

F

R

Q

N

D

I

F

S

S

M

L

P

S

I

I

A

P

H

S

Q

G

K

S

V

V

T

T

S

A

L

L

I

V

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

I

L

R

S

C

L

F

L

N

L

R

R

M

L

N

Y

D

D

L

P

V

A

S

S

G

G

C

-

R

-

I

-

E

-

G

-

S

T

L

I

T

S

F

L

H

D

G

G

R

H

D

D

I

I

Y

-

G

-

S

R

G

Q

A

L

D

N

A

P

V

V

D

W

I

L

R

R

M

S

Q

K

I

I

G

G

M

T

V

V

F

S

Q
|
Q

K

E

P

P

N

I

P

L

F

F

P

S

K

C

T

S

I

I

Y

A

E

E

N

N

I

I

A

A

Y

Y

G

G

P

A

R

D

I

D

H

P

G

S

I

S

R

V

D

T

R

A

A

E

E

E

L

I

D

Q

R

R

I

V

V

A

E

E

S

V

S

A

L

N

K

A

K

V

A

A

A

F

L

I

W

R

-

N

-

F

-

P

E

Q

E

G

V

F

K

N

T

T

R

V

L

V

H

G

D

E

S

K

A

G

L

V

G

L

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

T

A

L

L

A

L

V

K

N

N

P

P

E

K

I

I

I

L

L

L

M

L

D

D

E

E

P

A

C

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

A

Y

K

L

I

V

E

Q

D

E

L

A

I

L

D

D

E

R

L

L

K

M

K

D

D

G

Y

R

C

T

V

V

V

L

I

V

V

I

T

A

H

H

S

R

M

L

S

S

Q

-

A

T

A

I

R

K

V

N

S

A

D

N

F

M

T

V

A

A

F

V

M

L

Y

D

L

Q

G

G

D

K

L

I

I

T

E

E

F

Y

G

G

E

K

T

H

R

E

Q

E

I

L

F

L

E

S

N

K

P

P

binding phosphomethylphosphonic acid adenylate ester: Y348 (= Y16), S376 (= S41), G377 (= G42), G379 (= G44), K380 (= K45), S381 (= S46), T382 (= T47)
binding magnesium ion: S381 (= S46), Q422 (= Q94)

Sites not aligning to the query:

binding glycine: 17, 81

Q9NRK6 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2 from Homo sapiens (Human) (see 5 papers)
32% identity, 88% coverage: 18:239/253 of query aligns to 506:722/738 of Q9NRK6

query
sites
Q9NRK6

E

E

T

V

Q

P

A

I

L

F

R

Q

N

D

I

F

S

S

M

L

P

S

I

I

A

P

H

S

Q

G

K

S

V

V

T

T

S

A

L

L

I

V

G

G

P

P

S

S

G

G

C

S

G

G

K
|
K

S

S

T

T

L

V

I

L

R

S

C

L

F

L

N

L

R

R

M

L

N

Y

D
|
D

L

P

V

A

S

S

G

G

C

-

R

-

I

-

E

-

G

-

S

T

L

I

T

S

F

L

H

D

G

G

R

H

D

D

I

I

Y

-

G

-

S

R

G

Q

A

L

D

N

A

P

V

V

D

W

I

L

R

R

M

S

Q

K

I

I

G

G

M

T

V

V

F

S

Q

Q

K

E

P

P

N

I

P

L

F

F

P

S

K
x
C

T

S

I

I

Y

A

E

E

N

N

I

I

A

A

Y

Y

G

G

P

A

R

D

I

D

H

P

G

S

I

S

R

V

D

T

R

A

A

E

E

E

L

I

D

Q

R

R

I

V

V

A

E

E

S

V

S

A

L

N

K

A

K

V

A

A

A

F

L

I

W

R

-

N

-

F

-

P

E

Q

E

G

V

F

K

N

T

T

R

V

L

V

H

G

D

E

S

K

A

G

L

V

G

L

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

T

A

L

L

A

L

V

K

N

N

P

P

E

K

I

I

I

L

L

L

M

L

D
|
D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

A

Y

K

L

I

V

E

Q

D

E

L

A

I

L

D

D

E

R

L

L

K

M

K

D

D

G

Y

R

C

T

V

V

V

L

I

V

V

I

T

A

H

H

S

R

M

L

S

S

Q

T

A

-

A

I

R

K

V

N

S

A

D

N

F

M

T

V

A

A

F

V

M

L

Y

D

L

Q

G

G

D

K

L

I

I

T

E

E

F

Y

G

G

E

K

T

H

R

E

Q

E

I

L

F

L

E

S

N

K

P

P

K533 (= K45) mutation to E: Increases hemoglobin biosynthetic process.
D545 (= D57) to N: in dbSNP:rs35698797
C582 (≠ K101) mutation to G: Does not affect ATPase activity; when associated with S-215 and L-224. Activated by Zn (II) mesoporphyrin; when associated with S-215 and L-224.
S635 (= S151) mutation to R: Does not rescue hemoglobin and heme biosynthetic process.
Q638 (= Q154) mutation to H: Does not rescue hemoglobin and heme biosynthetic process.
D658 (= D174) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.
E659 (= E175) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.

Sites not aligning to the query:

150 A → S: in dbSNP:rs4148756
215 C→S: Does not affect ATPase activity; when associated with L-224 and G-582. Activated by Zn (II) mesoporphyrin; when associated with L-224 and G-582.
224 C→L: Does not affect ATPase activity; when associated withS-215 and G-582. Activated by Zn (II) mesoporphyrin; when associated with S-215 and G-582.
471 R → T: in a breast cancer sample; somatic mutation

6qexA Nanodisc reconstituted human abcb1 in complex with uic2 fab and taxol (see paper)
32% identity, 89% coverage: 7:230/253 of query aligns to 361:579/1182 of 6qexA

query
sites
6qexA

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Sites not aligning to the query:

binding cholesterol: 15, 18, 36, 43, 81, 86, 89, 189, 281, 745, 749, 751, 755, 757, 761, 762, 765, 769, 772, 775, 854, 857, 861, 864, 865
binding taxol: 38, 276, 279, 305, 312, 313, 316, 631, 852, 859, 889, 892, 896

7o9wA Encequidar-bound human p-glycoprotein in complex with uic2-fab (see paper)
32% identity, 89% coverage: 7:230/253 of query aligns to 348:566/1169 of 7o9wA

query
sites
7o9wA

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Sites not aligning to the query:

binding ~{N}-[2-[2-[4-[2-(6,7-dimethoxy-3,4-dihydro-1~{H}-isoquinolin-2-yl)ethyl]phenyl]-1,2,3,4-tetrazol-5-yl]-4,5-dimethoxy-phenyl]-4-oxidanylidene-2,3-dihydrochromene-2-carboxamide: 34, 38, 188, 195, 259, 262, 266, 292, 299, 303, 618, 625, 768, 839, 842, 846, 872, 876, 879, 883, 887

7a6fA Nanodisc reconstituted human abcb1 in complex with mrk16 fab and zosuquidar (see paper)
32% identity, 89% coverage: 7:230/253 of query aligns to 343:561/1164 of 7a6fA

query
sites
7a6fA

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Sites not aligning to the query:

binding cholesterol: 259, 266, 607, 615, 618, 646, 727, 731, 744, 747, 748
binding Zosuquidar: 34, 34, 254, 257, 287, 290, 294, 613, 764, 767, 838, 841, 874, 878, 879

7a6eA Nanodisc reconstituted human abcb1 in complex with mrk16 fab and tariquidar (see paper)
32% identity, 89% coverage: 7:230/253 of query aligns to 343:561/1164 of 7a6eA

query
sites
7a6eA

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Sites not aligning to the query:

binding cholesterol: 43, 68, 72, 153, 160, 167, 168, 171, 172, 259, 266, 267, 645, 727, 731, 732, 736, 843, 846
binding tariquidar: 34, 41, 183, 254, 257, 258, 261, 287, 298, 613, 616, 763, 837, 841, 866, 867, 871, 874, 878

7a6cA Nanodisc reconstituted human abcb1 in complex with mrk16 fab and elacridar (see paper)
32% identity, 89% coverage: 7:230/253 of query aligns to 343:561/1164 of 7a6cA

query
sites
7a6cA

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