SitesBLAST
Comparing WP_109969613.1 NCBI__GCF_003173355.1:WP_109969613.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
42% identity, 94% coverage: 14:374/384 of query aligns to 3:372/376 of O66442
- GT 96:97 (≠ GA 108:109) binding pyridoxal 5'-phosphate
- K242 (= K249) modified: N6-(pyridoxal phosphate)lysine
- T271 (= T276) binding pyridoxal 5'-phosphate
2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
42% identity, 94% coverage: 14:374/384 of query aligns to 2:371/375 of 2eh6A
- active site: F127 (= F135), E179 (= E187), D212 (= D220), Q215 (= Q223), K241 (= K249), T270 (= T276), R352 (= R355)
- binding pyridoxal-5'-phosphate: G95 (= G108), T96 (≠ A109), F127 (= F135), H128 (= H136), E179 (= E187), D212 (= D220), V214 (= V222), K241 (= K249)
8ht4B Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
43% identity, 93% coverage: 23:379/384 of query aligns to 19:390/390 of 8ht4B
Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
43% identity, 93% coverage: 22:379/384 of query aligns to 28:395/395 of Q5SHH5
- GT 113:114 (≠ GA 108:109) binding pyridoxal 5'-phosphate
- K254 (= K249) modified: N6-(pyridoxal phosphate)lysine
- T283 (= T276) binding pyridoxal 5'-phosphate
P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
40% identity, 96% coverage: 14:381/384 of query aligns to 34:428/429 of P73133
- Y39 (≠ F19) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
- S125 (= S107) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
- G126 (= G108) mutation to A: Loss of N-acetylornithine aminotransferase activity.
- A127 (= A109) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
- R163 (= R138) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
- E223 (= E192) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
- D251 (= D220) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
- Q254 (= Q223) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
- K280 (= K249) mutation to A: Loss of N-acetylornithine aminotransferase activity.
- T308 (= T276) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
- R402 (= R355) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.
1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
43% identity, 93% coverage: 22:379/384 of query aligns to 20:387/387 of 1wkhA
- active site: F132 (= F135), E184 (= E187), D217 (= D220), Q220 (= Q223), K246 (= K249), T275 (= T276), R363 (= R355)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ I48), S104 (= S107), G105 (= G108), T106 (≠ A109), F132 (= F135), S133 (≠ H136), E184 (= E187), E189 (= E192), D217 (= D220), I219 (≠ V222), K246 (= K249), R363 (= R355)
Sites not aligning to the query:
1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
43% identity, 93% coverage: 22:379/384 of query aligns to 20:387/387 of 1wkgA
- active site: F132 (= F135), E184 (= E187), D217 (= D220), Q220 (= Q223), K246 (= K249), T275 (= T276), R363 (= R355)
- binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ I48), G105 (= G108), T106 (≠ A109), F132 (= F135), S133 (≠ H136), R135 (= R138), E184 (= E187), D217 (= D220), I219 (≠ V222), Q220 (= Q223), K246 (= K249), G273 (= G274), T274 (≠ S275), T275 (= T276)
Sites not aligning to the query:
1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
43% identity, 93% coverage: 22:379/384 of query aligns to 20:387/387 of 1vefA
- active site: F132 (= F135), D217 (= D220), K246 (= K249), T275 (= T276), R363 (= R355)
- binding pyridoxal-5'-phosphate: G105 (= G108), T106 (≠ A109), F132 (= F135), S133 (≠ H136), E184 (= E187), D217 (= D220), I219 (≠ V222), K246 (= K249)
Sites not aligning to the query:
A0QYS9 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
44% identity, 96% coverage: 1:368/384 of query aligns to 1:373/390 of A0QYS9
- K304 (≠ E306) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P9WPZ7 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
45% identity, 90% coverage: 23:368/384 of query aligns to 28:383/400 of P9WPZ7
- K314 (= K296) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylthreonine; partial
7nncC Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal-5'-phosphate and 6-methoxyquinoline-3-carboxylic acid
45% identity, 90% coverage: 23:368/384 of query aligns to 22:377/391 of 7nncC
7nn4A Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal 5'-phosphate and 3-hydroxy-2-naphthoic acid.
45% identity, 90% coverage: 23:368/384 of query aligns to 22:377/391 of 7nn4A
Sites not aligning to the query:
Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
40% identity, 96% coverage: 9:375/384 of query aligns to 63:450/457 of Q9M8M7