SitesBLAST
Comparing WP_109969624.1 NCBI__GCF_003173355.1:WP_109969624.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2vr1A Crystal structure of biotin carboxylase from e. Coli in complex with atp analog, adpcf2p. (see paper)
53% identity, 90% coverage: 4:445/491 of query aligns to 2:441/444 of 2vr1A
- active site: K116 (= K118), K159 (= K160), D194 (≠ R197), H207 (= H210), R233 (= R236), T272 (= T275), E274 (= E277), E286 (= E289), N288 (= N291), R290 (= R293), E294 (= E297), R336 (= R339)
- binding phosphodifluoromethylphosphonic acid-adenylate ester: K159 (= K160), R165 (≠ I168), M167 (= M170), Y201 (= Y204), L202 (= L205), E274 (= E277), L276 (= L279), E286 (= E289), N288 (= N291), I435 (≠ T439)
4mv4A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and mg2 (see paper)
52% identity, 90% coverage: 4:445/491 of query aligns to 2:440/442 of 4mv4A
- active site: K116 (= K118), K159 (= K160), D193 (≠ R197), H206 (= H210), R232 (= R236), T271 (= T275), E273 (= E277), E285 (= E289), N287 (= N291), R289 (= R293), E293 (= E297), R335 (= R339)
- binding phosphomethylphosphonic acid adenylate ester: K159 (= K160), G164 (= G165), M166 (= M170), E198 (= E202), Y200 (= Y204), L201 (= L205), H233 (= H237), L275 (= L279), E285 (= E289)
- binding magnesium ion: E273 (= E277), E285 (= E289)
6oi8A Crystal structure of haemophilus influenzae biotin carboxylase complexed with 7-((1r,5s,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl)- 6-(2-chloro-6-(pyridin-3-yl)phenyl)pyrido[2,3-d]pyrimidin-2-amine (see paper)
51% identity, 90% coverage: 4:445/491 of query aligns to 2:438/440 of 6oi8A
- active site: K116 (= K118), K159 (= K160), D191 (≠ R197), H204 (= H210), R230 (= R236), T269 (= T275), E271 (= E277), E283 (= E289), N285 (= N291), R287 (= R293), E291 (= E297), R333 (= R339)
- binding 7-[(1R,5S,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl]-6-[2-chloro-6-(pyridin-3-yl)phenyl]pyrido[2,3-d]pyrimidin-2-amine: I157 (= I158), K159 (= K160), M164 (= M170), E196 (= E202), Y198 (= Y204), L199 (= L205), H204 (= H210), Q228 (= Q234), E271 (= E277), L273 (= L279), E283 (= E289), I432 (≠ T439)
4mv3A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and bicarbonate (see paper)
51% identity, 90% coverage: 4:445/491 of query aligns to 2:437/439 of 4mv3A
- active site: K116 (= K118), K159 (= K160), D190 (≠ R197), H203 (= H210), R229 (= R236), T268 (= T275), E270 (= E277), E282 (= E289), N284 (= N291), R286 (= R293), E290 (= E297), R332 (= R339)
- binding phosphomethylphosphonic acid adenylate ester: K159 (= K160), M163 (= M170), E195 (= E202), Y197 (= Y204), L198 (= L205), E270 (= E277), L272 (= L279), E282 (= E289)
- binding bicarbonate ion: R286 (= R293), Q288 (= Q295), V289 (= V296)
P24182 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Escherichia coli (strain K12) (see 3 papers)
54% identity, 90% coverage: 4:445/491 of query aligns to 2:443/449 of P24182
- R19 (= R21) mutation to E: Loss of homodimerization. No effect on ATP binding.
- E23 (= E25) mutation to R: Loss of homodimerization. No effect on ATP binding.
- K116 (= K118) binding ATP
- K159 (= K160) binding ATP
- GG 165:166 (= GG 166:167) binding ATP
- EKYL 201:204 (= EKYL 202:205) binding ATP
- H209 (= H210) binding ATP
- H236 (= H237) binding ATP
- K238 (= K239) binding hydrogencarbonate
- E276 (= E277) binding ATP; binding Mg(2+)
- E288 (= E289) binding ATP; binding Mg(2+)
- R292 (= R293) active site; binding hydrogencarbonate
- V295 (= V296) binding hydrogencarbonate
- E296 (= E297) mutation to A: Severe reduction in catalytic activity.
- R338 (= R339) binding biotin; binding hydrogencarbonate; mutation to A: Severe reduction in catalytic activity.
- F363 (≠ P365) mutation to A: Loss of homodimerization. No effect on ATP binding.
- R366 (= R368) mutation to E: Loss of homodimerization. No effect on ATP binding.
3rupA Crystal structure of e.Coli biotin carboxylase in complex with two adp and two ca ions (see paper)
54% identity, 90% coverage: 4:445/491 of query aligns to 2:443/444 of 3rupA
- active site: K116 (= K118), K159 (= K160), D196 (≠ R197), H209 (= H210), R235 (= R236), T274 (= T275), E276 (= E277), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
- binding adenosine-5'-diphosphate: Y82 (= Y84), G83 (= G85), K116 (= K118), K159 (= K160), G164 (= G165), G164 (= G165), G165 (= G166), G166 (= G167), R167 (≠ I168), M169 (= M170), F193 (= F194), E201 (= E202), K202 (= K203), Y203 (= Y204), L204 (= L205), H209 (= H210), Q233 (= Q234), H236 (= H237), K238 (= K239), L278 (= L279), E288 (= E289), R292 (= R293), V295 (= V296), E296 (= E297), R338 (= R339), D382 (= D384), I437 (≠ T439)
- binding calcium ion: E87 (= E89), E276 (= E277), E288 (= E289), E288 (= E289), N290 (= N291)
3g8cA Crystal structure of biotin carboxylase in complex with biotin, bicarbonate, adp and mg ion (see paper)
54% identity, 90% coverage: 4:445/491 of query aligns to 2:443/444 of 3g8cA
- active site: K116 (= K118), K159 (= K160), D196 (≠ R197), H209 (= H210), R235 (= R236), T274 (= T275), E276 (= E277), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
- binding adenosine-5'-diphosphate: I157 (= I158), K159 (= K160), G164 (= G165), M169 (= M170), E201 (= E202), K202 (= K203), Y203 (= Y204), L204 (= L205), Q233 (= Q234), H236 (= H237), L278 (= L279), E288 (= E289), I437 (≠ T439)
- binding bicarbonate ion: K238 (= K239), R292 (= R293), Q294 (= Q295), V295 (= V296), E296 (= E297)
- binding biotin: Y82 (= Y84), F84 (= F86), R292 (= R293), V295 (= V296), R338 (= R339), D382 (= D384)
- binding magnesium ion: E276 (= E277), E288 (= E289)
3jziA Crystal structure of biotin carboxylase from e. Coli in complex with benzimidazole series (see paper)
54% identity, 90% coverage: 4:445/491 of query aligns to 2:443/445 of 3jziA
- active site: K116 (= K118), K159 (= K160), D196 (≠ R197), H209 (= H210), R235 (= R236), T274 (= T275), E276 (= E277), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
- binding 7-amino-2-[(2-chlorobenzyl)amino]-1-{[(1S,2S)-2-hydroxycycloheptyl]methyl}-1H-benzimidazole-5-carboxamide: K116 (= K118), K159 (= K160), A160 (= A161), G164 (= G165), G165 (= G166), M169 (= M170), Y199 (≠ F200), E201 (= E202), K202 (= K203), Y203 (= Y204), H209 (= H210), Q233 (= Q234), H236 (= H237), L278 (= L279), I287 (≠ M288), E288 (= E289)
2w6oA Crystal structure of biotin carboxylase from e. Coli in complex with 4-amino-7,7-dimethyl-7,8-dihydro-quinazolinone fragment (see paper)
54% identity, 90% coverage: 4:445/491 of query aligns to 2:443/445 of 2w6oA
- active site: K116 (= K118), K159 (= K160), D196 (≠ R197), H209 (= H210), R235 (= R236), T274 (= T275), E276 (= E277), E288 (= E289), N290 (= N291), R292 (= R293), E296 (= E297), R338 (= R339)
- binding 4-amino-7,7-dimethyl-7,8-dihydroquinazolin-5(6H)-one: K159 (= K160), K202 (= K203), Y203 (= Y204), L204 (= L205), L278 (= L279), I437 (≠ T439)
2w6nA Crystal structure of biotin carboxylase from e. Coli in complex with amino-oxazole fragment series (see paper)
54% identity, 90% coverage: 4:445/491 of query aligns to 2:443/445 of 2w6nA