SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_109969686.1 NCBI__GCF_003173355.1:WP_109969686.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
42% identity, 85% coverage: 15:254/281 of query aligns to 11:259/304 of Q96XT4

query
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Q96XT4

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C12 (= C16) binding [4Fe-4S] cluster
C15 (= C19) binding [4Fe-4S] cluster
IGCS 44:47 (≠ IGCH 51:54) binding thiamine diphosphate
C46 (= C53) binding [4Fe-4S] cluster
D90 (= D95) binding Mg(2+)
GD 91:92 (= GD 96:97) binding thiamine diphosphate
N118 (= N123) binding Mg(2+)
V120 (= V125) binding Mg(2+)
GL 122:123 (= GL 127:128) binding thiamine diphosphate
C197 (= C202) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
42% identity, 85% coverage: 15:254/281 of query aligns to 8:256/301 of 5b46B

query
sites
5b46B

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binding magnesium ion: D87 (= D95), N115 (= N123), V117 (= V125)
binding iron/sulfur cluster: W8 (= W15), C9 (= C16), C12 (= C19), C43 (= C53), C194 (= C202), T196 (≠ S204), Y197 (≠ F205)
binding thiamine diphosphate: I41 (= I51), G42 (= G52), C43 (= C53), S44 (≠ H54), H62 (= H70), G86 (= G94), G88 (= G96), D89 (= D97), N115 (= N123), V117 (= V125), Y118 (= Y126), G119 (= G127), L120 (= L128), T121 (= T129)

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
41% identity, 91% coverage: 9:264/281 of query aligns to 5:269/305 of P72579

query
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P72579

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K49 (= K56) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L128) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
41% identity, 90% coverage: 13:264/281 of query aligns to 9:269/305 of Q96Y68

query
sites
Q96Y68

N

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C12 (= C16) binding [4Fe-4S] cluster
C15 (= C19) binding [4Fe-4S] cluster
C46 (= C53) binding [4Fe-4S] cluster
K49 (= K56) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D95) binding Mg(2+)
GD 91:92 (= GD 96:97) binding thiamine diphosphate
N118 (= N123) binding Mg(2+)
V120 (= V125) binding Mg(2+)
GL 122:123 (= GL 127:128) binding thiamine diphosphate
K125 (≠ T130) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ P178) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C202) binding [4Fe-4S] cluster

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
39% identity, 85% coverage: 15:254/281 of query aligns to 4:230/275 of 5b47B

query
sites
5b47B

W

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V

F

A

A

T

T

G

G

I

I

K

K

M

L

A

S

N

N

P

P

D

S

L

L

K

E

V

V

I

I

C

V

F

N

A

V

G

G

D
|
D

G
|
G

D
|
D

L

G

Y

L

A

G

E

I

G

G

L

M

D

G

H

H

L

F

I

V

H

H

A

L

A

G

K

R

R

R

N

N

I

I

D

D

I

I

T

A

V

V

I

L

C

V

H

H

N

N

N
|
N

R

G

V
|
V

Y
|
Y

G

G

L
|
L

T

Q

T

-

G

-

Q

-

Y

-

T

-

P

-

T

A

S

S

P

P

Y

T

G

L

Y

H

K

R

G

G

K

E

S

N

T

I

P

M

Q

D

G

A

L

-

H

-

E

-

Y

-

P

-

L

V

N

N

V

P

I

L

E

A

L

V

L

A

L

L

G

A

S

A

G

G

A

Y

T

T

F

F

V

V

S

A

R

R

A

G

Y

Y

T

A

R

Y

R

D

L

V

P

M

H

H

L

L

R

K

R

E

I

L

F

I

R

K

E

K

A

A

I

I

M

L

H

H

P

K

G

G

F

S

A

A

H

L

V

V

D

D

V

I

L

L

Q

Q

I

P

C
|
C

A

P

S

T

F

Y

F

R

-

V

-

Y

-

K

-

L

-

D

N

N

M

V

T

P

T

G

W

W

Y

-

D

-

E

D

R

P

I

V

Y

V

E

R

T

K

S

E

E

E

S

E

N

A

A

Q

G

K

L

K

F

F

E

E

F

Q

A

A

C

I

R

M

T

K

A

S

Q

Y

Q

E

W

W

N

G

Y

-

N

-

D

-

A

E

K

K

I

I

P

P

I

I

G

G

I

I

F

F

F

Y

K

Q

T

N

K

E

binding magnesium ion: D83 (= D95), N111 (= N123)
binding iron/sulfur cluster: C5 (= C16), C8 (= C19), C39 (= C53), C179 (= C202)
binding thiamine diphosphate: I37 (= I51), G38 (= G52), C39 (= C53), S40 (≠ H54), H58 (= H70), G84 (= G96), D85 (= D97), N111 (= N123), V113 (= V125), Y114 (= Y126), L116 (= L128)

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
39% identity, 91% coverage: 9:264/281 of query aligns to 1:251/286 of 5b48B

query
sites
5b48B

T

T

Q

P

A

Q

Q

W

N

N

T

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

N

N

F

F

A

G

I

I

Q

L

H

N

A

A

M

E

K

Q

F

Q

V

A

I

I

Q

V

E

E

L

L

E

-

A

-

E

-

G

G

I

V

S

D

R

T

D

K

Q

N

F

V

V

V

L

V

V

V

S

S

G

G

I
|
I

G
|
G

C
|
C

H
x
S

A

G

K

K

I

I

A

P

D

H

Y

F

F

T

Q

P

L

I

N

S

S

G

F

V

Y

H

S

T

L

L

H
|
H

G

G

R

R

T

A

I

I

P

A

V

F

A

A

T

T

G

G

I

I

K

K

M

L

A

S

N

N

P

P

D

D

L

L

K

V

V

V

I

I

C

V

F

N

A

G

G

G

D
|
D

G
|
G

D
|
D

L

L

Y

L

A

G

E

I

G

G

L

A

D

G

H

H

L

F

I

V

H

A

A

A

A

G

K

R

R

R

N

N

I

V

D

D

I

M

T

V

V

V

I

I

C

L

H

H

N

D

N
|
N

R

G

V
|
V

Y
|
Y

G
|
G

L
|
L

T

T

T

K

G

G

Q

Q

Y

A

T

S

P

P

T

T

S

L

P

K

Y

R

G

G

Y

E

K

N

G

I

K

N

S

D

T

A

P

-

Q

-

G

-

L

-

H

-

E

-

Y

-

P

-

L

V

N

N

V

P

I

I

E

A

L

L

L

A

L

I

G

S

S

S

G

G

A

Y

T

T

F

F

V

V

S

A

R

R

A

G

Y

Y

T

A

R

Y

R

D

L

V

P

K

H

H

L

L

R

K

R

E

I

L

F

I

R

K

E

S

A

A

I

I

M

K

H

H

P

K

G

G

F

L

A

A

H

L

V

I

D

D

V

V

L

L

Q

Q

I

P

C
|
C

A

P

S
x
T

F

Y

F

N

N

D

M

I

T

N

T

T

-

K

-

E

W

W

-

R

-

I

-

Y

-

K

-

L

-

D

-

T

-

L

-

P

-

D

Y

W

D

D

E

P

R

V

I

V

Y

K

E

K

T

P

S

E

E

E

S

V

N

N

A

E

G

K

L

I

F

-

E

K

F

R

A

A

C

I

R

D

T

K

A

S

Q

L

Q

E

W

W

N

-

Y

-

N

-

D

-

A

G

K

D

I

I

P

P

I

I

G

G

I

I

F

F

F

Y

K

Q

T

N

K

E

K

L

P

V

V

P

L

S

P

Y

E

E

K

E

K

R

I

I

K

K

binding magnesium ion: D84 (= D95), N112 (= N123), V114 (= V125)
binding iron/sulfur cluster: C8 (= C16), C11 (= C19), C42 (= C53), C183 (= C202), T185 (≠ S204)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I51), G41 (= G52), C42 (= C53), S43 (≠ H54), H59 (= H70), G85 (= G96), D86 (= D97), N112 (= N123), V114 (= V125), Y115 (= Y126), G116 (= G127), L117 (= L128)

6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
34% identity, 91% coverage: 9:264/281 of query aligns to 18:269/291 of 6n2oD

query
sites
6n2oD

T

S

Q

E

A

V

Q

P

N

T

T

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N

H

F

F

A

G

I

I

Q

L

H

N

A

G

M

V

K

Y

F

R

V

A

I

M

Q

A

E

E

L

L

E

-

A

-

E

-

G

G

I

I

S

D

R

S

D

T

Q

K

F

F

V

A

L

A

V

I

S

S

G

G

I
|
I

G
|
G

C
|
C

H
x
S

A

S

K
x
R

I

M

A

P

D

Y

Y

F

F

V

Q

D

L

S

N

Y

S

K

F

M

Y

H

S

T

L

L

H
|
H

G

G

R

R

T

A

I

G

P

A

V

V

A

A

T

T

G

G

I

T

K

Q

M

V

A

A

N

R

P

P

D

D

L

L

K

C

V

V

I

V

C

V

F

A

A

G

G

G

D
|
D

G
|
G

D
|
D

L

G

Y

F

A

S

E

I

G

G

L

G

D

G

H

H

L

M

I

P

H

H

A

M

A

A

K

R

R

K

N

N

I

V

D

N

I

M

T

T

V

Y

I

V

C

L

H

M

N

D

N
|
N

R

G

V
x
I

Y

Y

G
|
G

L
|
L

T
|
T

T
x
K

G

G

Q

Q

Y

Y

T

S

P

P

T

T

S

S

P

R

Y

P

G

E

Y

M

K

T

G

A

K

Y

S

T

T

T

P

P

Q

Y

G

G

L

G

H

P

E

E

Y

N

P

P

L

M

N

N

V

P

I

L

E

L

L

Y

L

M

L

L

G

T

S

Y

G

G

A

A

T

T

F

Y

V

V

S

A

R

Q

A

A

Y

F

T

A

R

G

R

K

L

P

P

K

H

D

L

C

R

A

R

E

I

L

F

I

R

K

E

G

A

A

I

M

M

E

H

H

P

E

G

G

F

F

A

A

H

Y

V

V

D

N

V

I

L

F

Q

S

I

Q

C
|
C

A

P

S
x
T

F
|
F

F

N

N

K

M

I

T

D

T

T

-

V

-

D

W

F

Y

Y

D

R

E

D

R

L

I

V

-

E

-

P

-

I

-

P

-

E

-

D

Y

H

E

D

T

T

S

S

E

D

S

L

N

G

A

A

G

A

L

M

F

-

E

E

F

L

A

A

C

R

R

R

T

P

A

G

Q

-

W

-

N

-

Y

-

N

-

D

-

A

G

K

K

I

A

P

P

I

T

G

G

I

L

F

L

F

Y

K

K

T

T

K

S

K

A

P

P

V

T

L

L

P

D

E

Q

K

N

K

L

I

A

K

K

binding magnesium ion: D101 (= D95), N129 (= N123), I131 (≠ V125)
binding succinyl-coenzyme a: I57 (= I51), R62 (≠ K56), L134 (= L128), K136 (≠ T130)
binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), C59 (= C53), C208 (= C202), T210 (≠ S204), F211 (= F205)
binding thiamine diphosphate: I57 (= I51), G58 (= G52), C59 (= C53), S60 (≠ H54), H76 (= H70), G102 (= G96), D103 (= D97), N129 (= N123), I131 (≠ V125), G133 (= G127), L134 (= L128), T135 (= T129)

6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
34% identity, 91% coverage: 9:264/281 of query aligns to 18:269/291 of 6n2oB

query
sites
6n2oB

T

S

Q

E

A

V

Q

P

N

T

T

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N

H

F

F

A

G

I

I

Q

L

H

N

A

G

M

V

K

Y

F

R

V

A

I

M

Q

A

E

E

L

L

E

-

A

-

E

-

G

G

I

I

S

D

R

S

D

T

Q

K

F

F

V

A

L

A

V

I

S

S

G

G

I
|
I

G
|
G

C
|
C

H
x
S

A

S

K
x
R

I

M

A

P

D

Y

Y

F

F

V

Q

D

L

S

N

Y

S

K

F

M

Y

H

S

T

L

L

H
|
H

G

G

R

R

T

A

I

G

P

A

V

V

A

A

T

T

G

G

I

T

K

Q

M

V

A

A

N

R

P

P

D

D

L

L

K

C

V

V

I

V

C

V

F

A

A

G

G

G

D
|
D

G
|
G

D
|
D

L

G

Y

F

A

S

E

I

G

G

L

G

D

G

H

H

L

M

I

P

H

H

A

M

A

A

K

R

R

K

N

N

I

V

D

N

I

M

T

T

V

Y

I

V

C

L

H

M

N

D

N
|
N

R

G

V
x
I

Y
|
Y

G
|
G

L
|
L

T
|
T

T
x
K

G

G

Q

Q

Y

Y

T

S

P

P

T

T

S

S

P

R

Y

P

G

E

Y

M

K

T

G

A

K
x
Y

S

T

T

T

P

P

Q

Y

G

G

L

G

H

P

E

E

Y

N

P

P

L

M

N

N

V

P

I

L

E

L

L

Y

L

M

L

L

G

T

S

Y

G

G

A

A

T

T

F

Y

V

V

S

A

R

Q

A

A

Y

F

T

A

R

G

R

K

L

P

P

K

H

D

L

C

R

A

R

E

I

L

F

I

R

K

E

G

A

A

I

M

M

E

H

H

P

E

G

G

F

F

A

A

H

Y

V

V

D

N

V

I

L

F

Q

S

I

Q

C
|
C

A

P

S
x
T

F
|
F

F

N

N

K

M

I

T

D

T

T

-

V

-

D

W

F

Y

Y

D

R

E

D

R

L

I

V

-

E

-

P

-

I

-

P

-

E

-

D

Y

H

E

D

T

T

S

S

E

D

S

L

N

G

A

A

G

A

L

M

F

-

E

E

F

L

A

A

C

R

R

R

T

P

A

G

Q

-

W

-

N

-

Y

-

N

-

D

-

A

G

K

K

I

A

P

P

I

T

G

G

I

L

F

L

F

Y

K

K

T

T

K

S

K

A

P

P

V

T

L

L

P

D

E

Q

K

N

K

L

I

A

K

K

binding 2-oxoglutaric acid: R62 (≠ K56), L134 (= L128)
binding coenzyme a: K136 (≠ T130), Y150 (≠ K144)
binding magnesium ion: D101 (= D95), N129 (= N123), I131 (≠ V125)
binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), C59 (= C53), C208 (= C202), T210 (≠ S204), F211 (= F205)
binding thiamine diphosphate: I57 (= I51), G58 (= G52), C59 (= C53), S60 (≠ H54), H76 (= H70), G102 (= G96), D103 (= D97), N129 (= N123), I131 (≠ V125), Y132 (= Y126), G133 (= G127), L134 (= L128), T135 (= T129)

6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
34% identity, 91% coverage: 9:264/281 of query aligns to 18:269/291 of 6n2nB

query
sites
6n2nB

T

S

Q

E

A

V

Q

P

N

T

T

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N
x
H

F

F

A

G

I

I

Q

L

H

N

A

G

M

V

K

Y

F

R

V

A

I

M

Q

A

E

E

L

L

E

-

A

-

E

-

G

G

I

I

S

D

R

S

D

T

Q

K

F

F

V

A

L

A

V

I

S

S

G

G

I
|
I

G
|
G

C
|
C

H
x
S

A

S

K

R

I

M

A

P

D

Y

Y

F

F

V

Q

D

L

S

N

Y

S

K

F

M

Y

H

S

T

L

L

H
|
H

G

G

R

R

T

A

I

G

P

A

V

V

A

A

T

T

G

G

I

T

K

Q

M

V

A

A

N

R

P

P

D

D

L

L

K

C

V

V

I

V

C

V

F

A

A

G

G
|
G

D
|
D

G
|
G

D
|
D

L

G

Y

F

A

S

E

I

G

G

L

G

D

G

H

H

L

M

I

P

H

H

A

M

A

A

K

R

R

K

N

N

I

V

D

N

I

M

T

T

V

Y

I

V

C

L

H

M

N

D

N
|
N

R

G

V
x
I

Y
|
Y

G
|
G

L
|
L

T
|
T

T

K

G

G

Q

Q

Y

Y

T

S

P

P

T

T

S

S

P

R

Y

P

G

E

Y

M

K

T

G

A

K

Y

S

T

T

T

P

P

Q

Y

G

G

L

G

H

P

E

E

Y

N

P

P

L

M

N

N

V

P

I

L

E

L

L

Y

L

M

L

L

G

T

S

Y

G

G

A

A

T

T

F

Y

V

V

S

A

R

Q

A

A

Y

F

T

A

R

G

R

K

L

P

P

K

H

D

L

C

R

A

R

E

I

L

F

I

R

K

E

G

A

A

I

M

M

E

H

H

P

E

G

G

F

F

A

A

H

Y

V

V

D

N

V

I

L

F

Q

S

I

Q

C
|
C

A

P

S
x
T

F
|
F

F

N

N

K

M

I

T

D

T

T

-

V

-

D

W

F

Y

Y

D

R

E

D

R

L

I

V

-

E

-

P

-

I

-

P

-

E

-

D

Y

H

E

D

T

T

S

S

E

D

S

L

N

G

A

A

G

A

L

M

F

-

E

E

F

L

A

A

C

R

R

R

T

P

A

G

Q

-

W

-

N

-

Y

-

N

-

D

-

A

G

K

K

I

A

P

P

I

T

G

G

I

L

F

L

F

Y

K

K

T

T

K

S

K

A

P

P

V

T

L

L

P

D

E

Q

K

N

K

L

I

A

K

K

binding magnesium ion: D101 (= D95), N129 (= N123), I131 (≠ V125)
binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), H30 (≠ N21), C59 (= C53), C208 (= C202), T210 (≠ S204), F211 (= F205)
binding thiamine diphosphate: I57 (= I51), G58 (= G52), C59 (= C53), S60 (≠ H54), H76 (= H70), G100 (= G94), D101 (= D95), G102 (= G96), D103 (= D97), N129 (= N123), I131 (≠ V125), Y132 (= Y126), G133 (= G127), L134 (= L128), T135 (= T129)

6lpiB Crystal structure of ahas holo-enzyme (see paper)
38% identity, 23% coverage: 71:134/281 of query aligns to 404:466/539 of 6lpiB

query
sites
6lpiB

G

G

R

F

T

G

I

L

P

P

V

A

A

A

T

I

G

G

I

A

K

A

M

L

A

A

N

N

P

P

D

D

L

R

K

K

V

V

I

L

C

C

F

F

A

S

G
|
G

D
|
D

G
|
G

D
x
S

L

L

Y

M

A
x
M

E

N

G

-

L

I

D

Q

H

E

L

M

I

A

H

T

A

A

A

S

K

E

R

N

N

Q

I

L

D

D

I

V

T

K

V

I

I

I

C

L

H

M

N

N

N
|
N

R

E

V
x
A

Y
x
L

G
|
G

L
|
L

T
x
V

T

H

G

Q

Q
|
Q

Y

Q

T

S

active site: D428 (= D95), N455 (= N123), A457 (≠ V125), L458 (≠ Y126), L460 (= L128), V461 (≠ T129), Q464 (= Q132)
binding magnesium ion: D428 (= D95), N455 (= N123)
binding thiamine diphosphate: G427 (= G94), D428 (= D95), G429 (= G96), S430 (≠ D97), M433 (≠ A100), N455 (= N123), A457 (≠ V125), L458 (≠ Y126), G459 (= G127), L460 (= L128), V461 (≠ T129)

Sites not aligning to the query:

active site: 27, 29, 30, 31, 32, 53, 76, 115, 116, 117, 165, 256, 283, 375, 401, 403
binding flavin-adenine dinucleotide: 155, 212, 213, 214, 236, 237, 238, 254, 256, 257, 276, 277, 278, 280, 282, 283, 300, 301, 319, 320, 380, 398
binding thiamine diphosphate: 53, 76, 79, 376, 377, 378, 401, 403

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
39% identity, 22% coverage: 89:149/281 of query aligns to 104:164/314 of 5exeC

query
sites
5exeC

V

I

I

I

C

V

F

M

A

A

G
|
G

D
|
D

G
|
G

D

G

L

A

Y

V

A

D

E

I

G

G

L

L

D

Q

H

A

L

L

I

S

H

A

A

M

A

L

K

Y

R

R

N

G

I

H

D
|
D

I

V

T

L

V

F

I

I

C

C

H
x
Y

N

D

N
|
N

R

E

V
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

T

G

G

I

Q

Q

Y

T

T

S

P

P

T

T

S

T

P

P

Y

Y

G

G

Y

A

K

N

G

T

K

T

S

F

T

T

P

P

Q

P

G

G

active site: N143 (≠ L128)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: G109 (= G94), D110 (= D95), G111 (= G96), Y136 (≠ H121), N138 (= N123), S140 (≠ V125), Y141 (= Y126), A142 (≠ G127), N143 (≠ L128), T144 (= T129)
binding magnesium ion: D110 (= D95), D130 (= D115), N138 (= N123), S140 (≠ V125)

Sites not aligning to the query:

5c4iC Structure of an oxalate oxidoreductase (see paper)
39% identity, 22% coverage: 89:149/281 of query aligns to 104:164/312 of 5c4iC

query
sites
5c4iC

V

I

I

I

C

V

F

M

A

A

G
|
G

D
|
D

G
|
G

D

G

L

A

Y

V

A

D

E

I

G

G

L

L

D

Q

H

A

L

L

I

S

H

A

A

M

A

L

K

Y

R

R

N

G

I

H

D

D

I

V

T

L

V

F

I

I

C

C

H
x
Y

N

D

N
|
N

R

E

V
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

T

G

G

I

Q

Q

Y

T

T

S

P

P

T

T

S

T

P

P

Y

Y

G

G

Y

A

K

N

G

T

K

T

S

F

T

T

P

P

Q

P

G

G

active site: N143 (≠ L128)
binding magnesium ion: D110 (= D95), N138 (= N123), S140 (≠ V125)
binding iron/sulfur cluster: A142 (≠ G127)
binding thiamine diphosphate: G109 (= G94), D110 (= D95), G111 (= G96), Y136 (≠ H121), N138 (= N123), S140 (≠ V125), Y141 (= Y126), A142 (≠ G127), N143 (≠ L128), T144 (= T129)

Sites not aligning to the query:

binding iron/sulfur cluster: 24, 27, 29, 52, 225, 226, 227
binding thiamine diphosphate: 50, 51, 52, 74

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
39% identity, 22% coverage: 89:149/281 of query aligns to 104:164/310 of 5exdF

query
sites
5exdF

V

I

I

I

C

V

F

M

A

A

G

G

D
|
D

G
|
G

D

G

L

A

Y

V

A

D

E

I

G

G

L

L

D

Q

H

A

L

L

I

S

H

A

A

M

A

L

K

Y

R

R

N

G

I

H

D

D

I

V

T

L

V

F

I

I

C

C

H
x
Y

N

D

N
|
N

R

E

V
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

T

G

G

I

Q

Q

Y

T

T

S

P

P

T

T

S

T

P

P

Y

Y

G

G

Y

A

K

N

G

T

K

T

S

F

T

T

P

P

Q

P

G

G

active site: N143 (≠ L128)
binding magnesium ion: D110 (= D95), N138 (= N123), S140 (≠ V125)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: D110 (= D95), G111 (= G96), Y136 (≠ H121), N138 (= N123), S140 (≠ V125), Y141 (= Y126), A142 (≠ G127), N143 (≠ L128), T144 (= T129)
binding iron/sulfur cluster: A142 (≠ G127)

Sites not aligning to the query:

Query Sequence

>WP_109969686.1 NCBI__GCF_003173355.1:WP_109969686.1
MTSEESLVTQAQNTWCPGCGNFAIQHAMKFVIQELEAEGISRDQFVLVSGIGCHAKIADY
FQLNSFYSLHGRTIPVATGIKMANPDLKVICFAGDGDLYAEGLDHLIHAAKRNIDITVIC
HNNRVYGLTTGQYTPTSPYGYKGKSTPQGLHEYPLNVIELLLGSGATFVSRAYTRRLPHL
RRIFREAIMHPGFAHVDVLQICASFFNMTTWYDERIYETSESNAGLFEFACRTAQQWNYN
DDAKIPIGIFFKTKKPVLPEKKIKKEVNRSNLVKTILDRAT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory