SitesBLAST
Comparing WP_110205269.1 NCBI__GCF_003194585.1:WP_110205269.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
P0AGF4 D-xylose-proton symporter; D-xylose transporter from Escherichia coli (strain K12) (see paper)
23% identity, 85% coverage: 66:443/447 of query aligns to 70:488/491 of P0AGF4
- G83 (= G79) mutation to A: Abolishes xylose transport.
- R133 (= R119) mutation R->C,H,L: Abolishes xylose transport.
- E153 (= E139) mutation to A: Abolishes xylose transport.
- R160 (= R146) mutation to A: Abolishes xylose transport.
- Q168 (≠ E154) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
- Q288 (vs. gap) mutation to A: Abolishes xylose transport.
- QQ 288:289 (vs. gap) binding beta-D-xylose
- Q289 (vs. gap) mutation to A: Strongly decreases xylose transport.
- N294 (= N255) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
- Y298 (≠ S260) mutation to A: Abolishes xylose transport.
- N325 (≠ M288) mutation to A: No effect on xylose transport.
- G340 (= G303) mutation to A: Abolishes xylose transport.
- R341 (= R304) mutation R->A,W: Abolishes xylose transport.
- W392 (≠ A349) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
- E397 (≠ T354) mutation to A: Abolishes xylose transport.
- R404 (= R364) mutation to A: Strongly decreases xylose transport.
- Q415 (vs. gap) binding beta-D-xylose
- W416 (vs. gap) mutation to A: Strongly decreases xylose transport.
Sites not aligning to the query:
- 24 F→A: Decreases xylose transport.
Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
27% identity, 57% coverage: 11:267/447 of query aligns to 39:280/444 of Q8NLB7
- D54 (= D26) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- D57 (≠ G28) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- R103 (= R77) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 309 W→V: Loss of transport activity.
- 312 D→A: Loss of transport activity.
- 313 R→A: Loss of transport activity.
- 317 mutation I->H,Y: Loss of transport activity.
- 386 R→A: Loss of transport activity.
P36035 Carboxylic acid transporter protein homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 48% coverage: 62:275/447 of query aligns to 186:402/616 of P36035
- K338 (vs. gap) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 9 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
26% identity, 80% coverage: 72:428/447 of query aligns to 85:440/448 of Q51955
- G85 (= G72) mutation to V: Abolishes 4-HBA transport and chemotaxis.
- D89 (= D76) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- G92 (= G79) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
- R124 (= R119) mutation to A: Abolishes 4-HBA transport.
- E144 (= E139) mutation to A: Strong decrease in 4-HBA transport.
- H183 (≠ R186) mutation to A: Decrease in 4-HBA transport and chemotaxis.
- D323 (= D300) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- H328 (≠ K305) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
- R386 (= R364) mutation to A: Strong decrease in 4-HBA transport.
- R398 (≠ L378) mutation to A: Abolishes 4-HBA transport.
Sites not aligning to the query:
- 41 mutation D->A,N: Abolishes 4-HBA transport.; D→E: Decrease in 4-HBA transport.
- 44 mutation D->A,N: Abolishes 4-HBA transport.; D→E: Decrease in 4-HBA transport.
- 444 H→A: No change in 4-HBA transport and chemotaxis.
Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 35% coverage: 66:223/447 of query aligns to 98:262/583 of Q9Y7Q9
Sites not aligning to the query:
- 267 modified: Phosphoserine
- 269 modified: Phosphoserine
- 289 modified: Phosphoserine
- 290 modified: Phosphoserine
- 292 modified: Phosphoserine
- 330 modified: Phosphoserine
P11168 Solute carrier family 2, facilitated glucose transporter member 2; Glucose transporter type 2, liver; GLUT-2 from Homo sapiens (Human) (see 6 papers)
23% identity, 57% coverage: 66:322/447 of query aligns to 106:383/524 of P11168
- T110 (vs. gap) to I: in dbSNP:rs5400
- V197 (≠ L158) to I: in NIDDM; abolishes transport activity of the transporter expressed in Xenopus oocytes; dbSNP:rs121909741
- I322 (≠ L259) mutation to V: Reduced fructose transport.
Sites not aligning to the query:
- 68 P → L: in dbSNP:rs7637863
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
34% identity, 21% coverage: 72:165/447 of query aligns to 78:159/452 of Q5EXK5
- D82 (= D76) mutation to A: Loss of activity.
Sites not aligning to the query:
- 311 V→W: Loss of activity.
- 314 D→A: Loss of activity.
8bvtA Cryo-em structure of rat slc22a6 bound to probenecid (see paper)
28% identity, 28% coverage: 66:192/447 of query aligns to 138:252/508 of 8bvtA
Sites not aligning to the query:
8bvsA Cryo-em structure of rat slc22a6 bound to tenofovir (see paper)
28% identity, 28% coverage: 66:192/447 of query aligns to 129:243/502 of 8bvsA
Sites not aligning to the query:
8bw7A Cryo-em structure of rat slc22a6 bound to alpha-ketoglutaric acid (see paper)
28% identity, 28% coverage: 66:192/447 of query aligns to 129:243/497 of 8bw7A
Sites not aligning to the query:
Q8VC69 Solute carrier family 22 member 6; Kidney-specific transport protein; Novel kidney transcript; mNKT; Organic anion transporter 1; mOAT1; Renal organic anion transporter 1; mROAT1 from Mus musculus (Mouse) (see 2 papers)
28% identity, 28% coverage: 66:192/447 of query aligns to 141:255/545 of Q8VC69
- C183 (≠ V116) mutation to A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-122; A-172; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
Sites not aligning to the query:
- 39 N→Q: Complete loss of PAH transport activity.
- 49 C→A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
- 56 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 86 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 91 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 107 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 122 C→A: Decreased cell surface expression level and PAH transport activity. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
- 434 C→A: Decreased cell surface expression level and PAH transport activity. 80% decrease of PAH transport activity; when associated with A-49; A-122 and A-183. Complete loss of PAH transport activity; when associated with A-49; A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402 and A-427.
A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
22% identity, 80% coverage: 72:430/447 of query aligns to 63:434/446 of A0A0H2VG78
- R102 (= R119) mutation to A: Loss of transport activity.
- I105 (≠ Q122) mutation to S: Affects symport activity. May function as an uniporter.
- E122 (= E139) mutation to A: Loss of transport activity.
- Q137 (≠ E154) mutation to A: Loss of transport activity.
- Q250 (≠ L251) mutation to A: Loss of transport activity.
- Q251 (≠ N252) mutation to A: Loss of transport activity.
- N256 (≠ T257) mutation to A: Loss of transport activity.
- W357 (vs. gap) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 22 D→N: Affects symport activity. May function as an uniporter.
Q9NSA0 Solute carrier family 22 member 11; Organic anion transporter 4; OAT4; Organic anion:dicarboxylate exchanger OAT4 from Homo sapiens (Human) (see 3 papers)
25% identity, 69% coverage: 66:374/447 of query aligns to 155:471/550 of Q9NSA0
- G241 (= G164) mutation G->L,S,V: Strongly reduced cell surface expression and estrone 3-sulfate transport.
- H305 (≠ A215) mutation to A: Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-52; A-83 and A-469.
- G400 (= G296) mutation G->L,S,V: Strongly reduced cell surface expression and estrone 3-sulfate transport.
- H469 (≠ Y372) mutation to A: Slightly reduced estrone 3-sulfate transport. Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-52; A-83 and A-305.
Sites not aligning to the query:
- 39 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface location; when associated with Q-56; Q-63 and Q-99.
- 47 H→A: Reduced cell surface expression and estrone 3-sulfate transport. Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-52; A-83; A-305 and A-469.
- 52 H→A: Slightly reduced estrone 3-sulfate transport. Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-83; A-305 and A-469.
- 56 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-63 and Q-99.
- 63 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-56 and Q-99.
- 83 H→A: Reduced cell surface expression and estrone 3-sulfate transport; when associated with A-47; A-52; A-305 and A-469.
- 99 N→Q: No visible effect on N-glycosylation. Loss of N-glycosylation and of cell surface expression; when associated with Q-39; Q-56 and Q-63.
O15244 Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2 from Homo sapiens (Human) (see 8 papers)
24% identity, 39% coverage: 66:241/447 of query aligns to 162:331/555 of O15244
- M165 (= M69) to I: lower Vmax for MPP(+) transport; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177507
- Y169 (≠ P73) mutation to F: No change in TEA uptake.
- T201 (= T113) to M: in dbSNP:rs145450955
- Y241 (≠ L153) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377.
- Y257 (≠ F161) mutation to F: No change in TEA uptake.
- S270 (≠ A174) to A: decreased Ki value for TBA inhibition of MPP(+); no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs316019
- Y279 (≠ W183) mutation to F: No change in TEA uptake.
- Y280 (≠ G184) mutation to F: No change in TEA uptake.
- P284 (= P188) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
- P---ESPR 284:288 (≠ PFLLAAPL 188:195) Proline-rich sequence
- S286 (≠ A193) mutation to A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation.
- P287 (= P194) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
Sites not aligning to the query:
- 54 P → S: in dbSNP:rs8177504
- 73 Y→F: No change in TEA uptake.
- 92 Y→F: No change in TEA uptake.
- 128 Y→F: No change in TEA uptake.
- 362 Y→F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377.
- 377 Y→F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362.
- 400 R → C: lower Vmax and reduced Ki value for TBA inhibition of MPP(+); lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro); no change in transport efficiency (Vmax/Km) and clearance of salsolinol; dbSNP:rs8177516
- 432 K → Q: lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177517
- 458 Y→F: No change in TEA uptake.
- 544 Y→F: No change in TEA uptake.
Query Sequence
>WP_110205269.1 NCBI__GCF_003194585.1:WP_110205269.1
MSTPTAPAVRRAIAASAIGNMTEWLDYGLYAYGVTYISAALFPGDTEQATLFALATFAIS
FLVRPLGGMFWGPLGDRVGRKAVLAVTILLMAGATLGVGLVPTYDRIGFWAPTLLVALRL
VQGFSTGGEYGGAATFMAEYAPDRRRGFLGSFLEFGTLTGFSLGALLMLGFSLALDDTAM
QAWGWRVPFLLAAPLGVIGLWLRSRAEDSPVFEEAAEERAVDEEPAVVRLRELVADHGRP
LLLLGALVMTLNVVNYTLLSYMPTYLEAEIGLSTDQSLMVPVIGMLTMMLFLPFAGHLSD
RVGRKPLWWVSVTGLFIAAVPMFLLMATGLLGAVIGFAVLGLLYVPQLATISSTFPAMFP
TQVRFAGFAIAYNVSTSLFGGTAPALNDWLVGLTGSALVPAFYMMLACAVGALALPAVVE
TAGVSLRGTEVPGTPESERELESLETT
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory