SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_110207905.1 NCBI__GCF_003194585.1:WP_110207905.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
63% identity, 98% coverage: 7:635/644 of query aligns to 12:648/651 of P9WQD1

query
sites
P9WQD1

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K617 (= K604) modified: N6-acetyllysine; mutation to R: Complete loss of acetyl-coenzyme A synthetase activity.

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
53% identity, 95% coverage: 21:631/644 of query aligns to 24:635/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G259) mutation to I: Loss of activity.
G266 (= G262) mutation to I: Great decrease in activity.
K269 (= K265) mutation to G: Great decrease in activity.
E414 (= E411) mutation to Q: Great decrease in activity.

8rplB Amp-forming acetyl-coa synthetase from chloroflexota bacterium with bound acetyl amp (see paper)
51% identity, 96% coverage: 3:623/644 of query aligns to 3:630/630 of 8rplB

query
sites
8rplB

E

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N

N

V

I

T

T

E

A

-

Y

-

M

-

K

-

S

-

K

-

G

-

F

-

D

-

D

-

Y

E

E

A

A

Y

F

A

Y

R

R

-

W

A

S

E

L

A

A

D

N

R

R

E

F

G

E

F

F

W

W

A

N

E

D

Q

M

A

A

E

K

R

E

L

L

D

H

W

W

G

F

Q

E

K

P

W

W

D

K

R

S

V

T

L

F

D

E

W

W

D

T

N

D

P

K

P

P

F

F

A

F

K

K

W

W

F

F

T

T

G

D

G

G

T

K

I

F

N

N

A

I

A

A

V

Y

N

N

C

C

V

L

D

D

R

R

H

Y

V

M

T

G

A

T

G

P

R

I

G

E

E

D

K

K

V

V

A

A

I

F

H

Y

W

W

V

E

G

G

E

D

P

-

E

D

D

G

D

S

T

S

R

R

D

A

I

Y

T

T

Y

Y

A

K

Q

E

L

M

Q

Y

D

V

E

L

V

T

N

N

R

R

A

V

A

A

N

K

A

V

L

L

T

Q

E

N

L

Q

G

G

V

V

A

K

K

K

G

G

D

D

R

R

V

V

A

A

I

I

Y

Y

L

M

P

P

M

M

I

I

P

P

E

E

A

M

V

A

V

A

T

S

M

V

L

L

A

A

C

C

A

A

R

R

L

L

G

G

A

A

P

P

H

H

T

M

V

V

V

V

F

F

G

G

G

G

F

F

S

A

A

A

D

S

A

S

L

L

A

R

S

D

R

R

I

M

V

N

D

D

C

C

G

D

A

A

R

K

V

V

V

L

V

I

T

T

A

A

D

D

G

G

G

G

Y

Y

R

R

R

G

G

G

A

K

P

V

S

I

A

E

L

L

K

K

P

K

A

I

V

A

D

D

E

E

A

A

V

V

A

A

K

E

A

T

D

P

G

-

L

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I

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E

H

K

V

V

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F

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Q

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G

G

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V

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D

A

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R

D

D

L

V

W

Y

W

L

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V

L

L

M

L

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N

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-

I

-

P

Q

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D

P

T

Q

V

H

V

D

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A

C

E

E

L

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H

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D

D

A

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E

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D

P

M

L

L

Y

Y

V

I

M

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

V

H

H

T

V

T

H

G

G

G

G

Y

Y

L

A

V

V

G

G

T

C

A

Y

Y

A

S

T

H

T

W

K

A

F

V

V

F

F

D

D

L

I

K

K

A

P

D

-

T

S

D

D

V

V

Y

F

W

W

C

C

T

T

A

A

D

D

I

I

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

L

T

V

I

Y

Y

G

G

P

P

L

M

A

M

N

N

G

A

A

A

T

S

Q

I

V

V

L

L

Y

F

E

E

G

G

T

I

P

P

D

T

S

Y

P

P

H

A

K

A

G

D

R

R

W

F

W

W

E

S

I

I

I

V

E

E

K

K

Y

Y

G

K

V

V

T

N

I

I

F

I

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

T

S

F

L

M

M

K

R

W

F

G

G

N

E

D

E

I

L

P

P

A

A

K

R

F

H

D

D

L

L

S

S

S

S

I

L

R

R

V

I

L

L

G

G

S

T

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

Y

W

V

M

W

W

Y

Y

R

R

S

K

T

N

I

I

G

G

G

H

D

N

R

E

A

L

P

P

V

I

V

M

D

D

T
|
T

W
|
W

W
|
W

Q
|
Q

T
|
T

E

E

T

T

G

G

Q

M

I

I

M

L

I

I

T

S

P

P

M

T

P

P

G

-

V

I

T

L

H

P

A

L

K

K

P

P

G

G

S

S

A

A

M

S

R

R

P

P

L

L

P

P

G

T

I

I

V

E

A

A

D

D

V

V

V

V

N

N

D

K

E

D

G

G

E

K

S

P

V

V

P

G

D

P

G

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S

H

G

G

G

G

Y

F

L

L

I

I

I

I

R

R

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H

P

P

W

W

P

P

A

A

M

Q

L

M

R

R

T

T

V

I

W

F

G

G

D

D

D

P

E

D

R

R

Y

Y

K

K

D

-

T

T

Y

Y

W

W

S

E

R

T

W

I

P

P

G

D

V

V

Y

Y

F

F

A

A

G

G

D
|
D

G

A

A

A

K

T

K

M

D

D

E

K

D

M

G

G

D

Y

I

F

W

R

V

I

L

Q

G

G

R
|
R

V

V

D

D

D

D

V

V

M

I

N
x
K

V

V

S

S

G

G

H

H

R
|
R

L

L

S

G

T

S

T

M

E

E

I

I

E

E

S

S

A

S

L

L

V
|
V

S

S

H
|
H

P

P

K

A

V

V

A

A

E

E

A

A

A

A

V

A

V

I

G

G

A

K

T

P

D

D

E

E

T

V

T

K

G

G

Q

E

A

H

V

V

C

K

A

V

F

F

V

V

I

I

L

L

R

R

E

N

S

G

A

V

G

-

D

E

G

P

G

T

D

E

E

S

I

L

V

A

A

V

E

E

L

L

R

K

N

R

H

H

V

V

A

R

K

T

E

L

I

V

G

G

A

P

I

L

A

A

K

T

P

P

R

D

Q

E

V

L

M

E

I

F

V

V

P

T

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K

K

I

I

M

M

R

R

R

R

L

V

L

V

R

R

D

A

V

R

A

E

E

L

N

G

R

E

E

P

I

V

G

G

D

D

V

I

T

T

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] ethanoate: G391 (= G381), E392 (= E382), P393 (= P383), T416 (= T406), W417 (= W407), W418 (= W408), Q419 (= Q409), T420 (= T410), D502 (= D494), R517 (= R509), K523 (≠ N515), R528 (= R520)
binding magnesium ion: V539 (= V531), H541 (= H533)

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
52% identity, 95% coverage: 21:631/644 of query aligns to 25:638/652 of P27550

query
sites
P27550

E

E

E

A

A

M

Y

Y

A

Q

R

Q

A

S

E

I

A

N

D

V

R

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

E

K

R

I

L

L

D

D

W

W

G

I

Q

K

K

P

W

Y

D

Q

R

K

V

V

L

-

D

-

W

-

D

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

T

E

G

D

G

G

T

T

I

L

N

N

A

L

A

A

V

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

T

Q

A

E

G

-

R

N

G

G

E

D

K

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

P

D

E

A

D

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

K

Q

E

L

L

Q

H

D

R

E

D

V

V

N

C

R

R

A

F

A

A

N

N

A

T

L

L

T

L

E

E

L

L

G

G

V

I

A

K

K

K

G

G

D

D

R

V

V

V

A

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

V

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

L

I

G

G

A

A

P

V

H

H

T

S

V

V

V

I

F

F

G

G

G

G

F

F

S

S

A

P

D

E

A

A

L

V

A

A

S

G

R

R

I

I

V

I

D

D

C

S

G

N

A

S

R

R

V

L

V

V

V

I

T

T

A

S

D

D

G

E

G

G

Y

V

R

R

R

A

G

G

A

R

P

S

S

I

A

P

L

L

K

K

P

K

A

N

V

V

D

D

E

D

A

A

V

L

A

K

K

N

A

P

D

N

-

V

G

T

L

S

V

V

E

E

H

H

V

V

V

V

V

V

V

L

Q

K

R

R

T

T

G

G

Q

G

E

K

V

I

A

D

F

W

D

Q

E

E

T

G

R

R

D

D

L

L

W

W

W

W

H

H

E

D

L

L

M

V

G

E

R

Q

Q

A

S

S

P

D

Q

Q

H

H

D

Q

A

A

E

E

L

E

H

M

D

N

A

A

E

E

H

D

P

P

L

L

Y

F

V

I

M

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

T

T

G

G

G

G

Y

Y

L

L

V

V

G

Y

T

A

A

A

Y

L

S

T

H

F

W

K

A

Y

V

V

F

F

D

D

L

Y

K

H

A

P

D

G

T

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

Q

T

V

L

L

M

Y

F

E

E

G

G

T

V

P

P

D

N

S

W

P

P

H

T

K

P

G

A

R

R

W

M

W

A

E

Q

I

V

I

V

E

D

K

K

Y

H

G

Q

V

V

T

N

I

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

D

K

I

A

P

I

A

E

K

G

F

T

D

D

L

R

S

S

S

S

I

L

R

R

V

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

Y

W

V

E

W

W

Y

Y

R

W

S

K

T

K

I

I

G

G

G

N

D

E

R

K

A

C

P

P

V

V

V

V

D

D

T

T

W

W

W

W

Q

Q

T

T

E

E

T

T

G

G

Q

G

I

F

M

M

I

I

T

T

P

P

M

L

P

P

G

G

V

A

T

T

H

E

A

L

K

K

P

A

G

G

S

S

A

A

M

T

R

R

P

P

L

F

P

F

G

G

I

V

V

Q

A

P

D

A

V

L

V

V

N

D

D

N

E

E

G

G

E

N

S

P

V

L

P

E

D

G

G

A

S

T

G

E

G

G

Y

S

L

L

I

V

I

I

R

T

E

D

P

S

W

W

P

P

A

G

M

Q

L

A

R

R

T

T

V

L

W

F

G

G

D

D

D

H

E

E

R

R

Y

F

K

E

D

Q

T

T

Y

Y

W

F

S

S

R

T

W

F

P

K

G

N

V

M

Y

Y

F

F

A

S

G

G

D

D

G

G

A

A

K

R

K

R

D

D

E

E

D

D

G

G

D

Y

I

Y

W

W

V

I

L

T

G

G

R

R

V

V

D

D

D

D

V

V

M

L

N

N

V

V

S

S

G

G

H

H

R

R

L

L

S

G

T

T

T

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

A

A

V

V

V

V

G

G

A

I

T

P

D

H

E

N

T

I

T

K

G

G

Q

Q

A

A

V

I

C

Y

A

A

F

Y

V

V

I

T

L

L

R

N

E

H

S

G

A

E

G

-

D

E

G

P

G

S

D

P

E

E

I

L

V

Y

A

A

E

E

L

V

R

R

N

N

H

W

V

V

A

R

K

K

E

E

I

I

G

G

A

P

I

L

A

A

K

T

P

P

R

D

Q

V

V

L

M

H

I

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

L

I

L

L

R

R

D

K

V

I

A

A

-

A

-

G

E

D

N

T

R

S

E

N

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

K609 (= K604) modified: N6-acetyllysine; by autocatalysis

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
51% identity, 95% coverage: 21:631/644 of query aligns to 21:632/641 of 2p20A

query
sites
2p20A

E

E

E

T

A

K

Y

Y

A

K

R

Q

A

S

E

I

A

N

D

D

R

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

E

K

R

I

L

L

D

D

W

W

G

I

Q

T

K

P

W

Y

D

Q

R

K

V

V

L

-

D

-

W

-

D

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

T

E

G

D

G

G

T

T

I

L

N

N

A

L

A

A

V

A

N

N

C

C

V

L

D

D

R

R

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H

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Q

A

E

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-

R

N

G

G

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K

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T

A

A

I

I

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I

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G

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D

E

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I

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Y

Y

A

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L

L

Q

H

D

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D

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V

N

C

R

R

A

F

A

A

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N

A

T

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L

T

L

E

D

L

L

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A

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K

K

G

G

D

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R

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A

A

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M

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E

A

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A

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L

A

A

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C

A

A

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A

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A

A

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A

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A

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G

A

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L

K

K

P

K

A

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D

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E

D

A

A

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A

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K

N

A

P

D

N

-

V

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P

P

Q

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A

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L

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D

N

A

A

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E

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D

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P

L

L

Y

F

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I

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L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

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K

G

G

I

V

L

L

H

H

T

T

T

T

G

G

G

G

Y

Y

L

L

V

V

G

Y

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A

A

A

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K

A

Y

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F

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D

L

Y

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A

P

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-

D

D

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I

Y

Y

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C

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A

A

D

D

I

V

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G

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W

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V

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T

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G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

Q

T

V

L

L

M

Y

F

E

E

G

G

T

V

P

P

D

N

S

W

P

P

H

T

K

P

G

A

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R

W

M

W

C

E

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I

V

I

V

E

D

K

K

Y

H

G

Q

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V

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N

I

I

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Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

D

K

I

A

P

I

A

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K

G

F

T

D

D

L

R

S

S

S

S

I

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

Y

W

V

E

W

W

Y

Y

R

W

S

K

T

K

I

I

G

G

G

K

D

E

R

K

A

C

P

P

V

V

V

V

D

D

T
|
T

W
|
W

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

Q

G

I

F

M

M

I

I

T

T

P

P

M

L

P

P

G

G

V

A

T

I

H

E

A

L

K

K

P

A

G

G

S

S

A

A

M

T

R

R

P

P

L

F

P

F

G

G

I

V

V

Q

A

P

D

A

V

L

V

V

N

D

D

N

E

E

G

G

E

H

S

P

V

Q

P

E

D

G

G

A

S

T

G

E

G

G

Y

N

L

L

I

V

I

I

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T

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D

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S

W

W

P

P

A

G

M

Q

L

A

R

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T

T

V

L

W

F

G

G

D

D

D

H

E

E

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R

Y

F

K

E

D

Q

T

T

Y

Y

W

F

S

S

R

T

W

F

P

K

G

N

V

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

K

R

K

R

D

D

E

E

D

D

G

G

D

Y

I

Y

W

W

V
x
I

L

T

G

G

R
|
R

V

V

D

D

D

D

V

V

M

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

L

L

S

G

T

T

T

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

A

A

V

V

V

V

G

G

A

I

T

P

D

H

E

A

T

I

T

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G

G

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Q

A

A

V

I

C

Y

A

A

F

Y

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V

I

T

L

L

R

N

E

H

S

G

A

E

G

-

D

E

G

P

G

S

D

P

E

E

I

L

V

Y

A

A

E

E

L

V

R

R

N

N

H

W

V

V

A

A

K

K

E

E

I

I

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G

A

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I

L

A

A

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D

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V

L

M

H

I

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

A

N

G

R

D

E

T

I

S

G

N

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

active site: T260 (= T257), T412 (= T410), E413 (= E411), N517 (= N515), R522 (= R520), K605 (= K604)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G381), E384 (= E382), P385 (= P383), T408 (= T406), W409 (= W407), W410 (= W408), Q411 (= Q409), T412 (= T410), D496 (= D494), I508 (≠ V506), R511 (= R509), R522 (= R520)

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
51% identity, 95% coverage: 21:631/644 of query aligns to 25:638/652 of Q8ZKF6

query
sites
Q8ZKF6

E

E

E

T

A

K

Y

Y

A

K

R

Q

A

S

E

I

A

N

D

D

R

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

E

K

R

I

L

L

D

D

W

W

G

I

Q

T

K

P

W

Y

D

Q

R

K

V

V

L

-

D

-

W

-

D

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

T

E

G

D

G

G

T

T

I

L

N

N

A

L

A

A

V

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

T

Q

A

E

G

-

R

N

G

G

E

D

K

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

P

D

E

T

D

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

Q

H

D

R

E

D

V

V

N

C

R

R

A

F

A

A

N

N

A

T

L

L

T

L

E

D

L

L

G

G

V

I

A

K

K

K

G

G

D

D

R

V

V

V

A

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

V

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

L

I

G

G

A

A

P

V

H

H

T

S

V

V

V

I

F

F

G

G

G

G

F

F

S

S

A

P

D

E

A

A

L

V

A

A

S

G

R

R

I

I

V

I

D

D

C

S

G

S

A

S

R

R

V

L

V

V

V

I

T

T

A

A

D

D

G

E

G

G

Y

V

R

R

R

A

G

G

A
x
R

P

S

S

I

A

P

L

L

K

K

P

K

A

N

V

V

D

D

E

D

A

A

V

L

A

K

K

N

A

P

D

N

-

V

G

T

L

S

V

V

E

E

H

H

V

V

V

I

V

V

V

L

Q

K

R

R

T

T

G

G

Q

S

E

D

V

I

A

D

F

W

D

Q

E

E

T

G

R

R

D

D

L

L

W

W

W

W

H

R

E

D

L

L

M

I

G

E

R

K

Q

A

S

S

P

P

Q

E

H

H

D

Q

A

P

E

E

L

A

H

M

D

N

A

A

E

E

H

D

P

P

L

L

Y

F

V

I

M

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

T

T

G

G

G

G

Y

Y

L

L

V

V

G

Y

T

A

A

A

Y

T

S

T

H

F

W

K

A

Y

V

V

F

F

D

D

L

Y

K

H

A

P

D

G

T

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

Q

T

V

L

L

M

Y

F

E

E

G

G

T

V

P

P

D
x
N

S

W

P

P

H

T

K

P

G

A

R

R

W

M

W

C

E

Q

I

V

I

V

E

D

K

K

Y

H

G

Q

V

V

T

N

I

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

D

K

I

A

P

I

A

E

K

G

F

T

D

D

L

R

S

S

S

S

I

L

R

R

V

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

Y

W

V

E

W

W

Y

Y

R

W

S

K

T

K

I

I

G

G

G

K

D

E

R

K

A

C

P

P

V

V

V

V

D

D

T

T

W

W

W

W

Q

Q

T

T

E

E

T

T

G

G

Q

G

I

F

M

M

I

I

T

T

P

P

M

L

P

P

G

G

V

A

T

I

H

E

A

L

K

K

P

A

G

G

S

S

A

A

M

T

R

R

P

P

L

F

P

F

G

G

I

V

V

Q

A

P

D

A

V

L

V

V

N

D

D

N

E

E

G

G

E

H

S

P

V

Q

P

E

D

G

G

A

S

T

G

E

G

G

Y

N

L

L

I

V

I

I

R

T

E

D

P

S

W

W

P

P

A

G

M

Q

L

A

R

R

T

T

V

L

W

F

G

G

D

D

D

H

E

E

R

R

Y

F

K

E

D

Q

T

T

Y

Y

W

F

S

S

R

T

W

F

P

K

G

N

V

M

Y

Y

F

F

A

S

G

G

D

D

G

G

A

A

K

R

K

R

D

D

E

E

D

D

G

G

D

Y

I

Y

W

W

V

I

L

T

G

G

R

R

V

V

D
|
D

D

D

V

V

M

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

L

L

S

G

T

T

T

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

A

A

V

V

V

V

G

G

A

I

T

P

D

H

E

A

T

I

T

K

G

G

Q

Q

A

A

V

I

C

Y

A

A

F

Y

V

V

I

T

L

L

R

N

E

H

S

G

A

E

G

-

D

E

G

P

G

S

D

P

E

E

I

L

V

Y

A

A

E

E

L

V

R

R

N

N

H

W

V

V

A
x
R

K

K

E

E

I

I

G

G

A

P

I

L

A

A

K

T

P

P

R

D

Q

V

V

L

M

H

I

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

L

I

L

L

R

R

D

K

V

I

A

A

-

A

-

G

E

D

N

T

R

S

E

N

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

R194 (≠ A188) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T305) binding CoA
N335 (≠ D329) binding CoA
A357 (= A351) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D511) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S517) binding CoA
G524 (= G518) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R520) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ A579) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K604) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
51% identity, 95% coverage: 21:631/644 of query aligns to 21:631/640 of 5jrhA

query
sites
5jrhA

E

E

E

T

A

K

Y

Y

A

K

R

Q

A

S

E

I

A

N

D

D

R

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

E

K

R

I

L

L

D

D

W

W

G

I

Q

T

K

P

W

Y

D

Q

R

K

V

V

L

-

D

-

W

-

D

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

T

E

G

D

G

G

T

T

I

L

N

N

A

L

A

A

V

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

T

Q

A

E

G

-

R

N

G

G

E

D

K

R

V

T

A

A

I

I

H

I

W
|
W

V

E

G

G

E

D

P

D

E

T

D

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

Q

H

D

R

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D

V

V

N

C

R

R

A

F

A

A

N

N

A

T

L

L

T

L

E

D

L

L

G

G

V

I

A

K

K

K

G

G

D

D

R

V

V

V

A

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E
|
E

A

A

V

A

V

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

L

I

G

G

A

A

P

V

H

H

T

S

V

V

V

I

F
|
F

G
|
G

G
|
G

F

F

S

S

A

P

D

E

A

A

L

V

A

A

S
x
G

R

R

I

I

V

I

D

D

C

S

G

S

A

S

R

R

V

L

V

V

V

I

T

T

A

A

D

D

G

E

G

G

Y

V

R
|
R

R

A

G

G

A

R

P

S

S

I

A

P

L

L

K

K

P

K

A

N

V

V

D

D

E

D

A

A

V

L

A

K

K

N

A

P

D

N

-

V

G

T

L

S

V

V

E

E

H

H

V

V

V

I

V

V

V

L

Q

K

R

R

T

T

G

G

Q

S

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D

V

I

A

D

F

W

D

Q

E

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G

R

R

D

D

L

L

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W

W

W

H

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D

L

L

M

I

G

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K

Q

A

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S

P

P

Q

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H

H

D

Q

A

P

E

E

L

A

H

M

D

N

A

A

E

E

H

D

P

P

L

L

Y

F

V

I

M

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

I

V

L

L

H

H

T

T

T

T

G

G

G

G

Y

Y

L

L

V

V

G

Y

T

A

A

A

Y

T

S

T

H

F

W

K

A

Y

V

V

F

F

D

D

L

Y

K

H

A

P

D

G

T

-

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

L

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

Q

T

V

L

L

M

Y

F

E

E

G

G

T

V

P

P

D

N

S

W

P

P

H

T

K

P

G

A

R

R

W

M

W

C

E

Q

I

V

I

V

E

D

K

K

Y

H

G

Q

V

V

T

N

I

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

N

D

D

K

I

A

P

I

A

E

K

G

F

T

D

D

L

R

S

S

S

S

I

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

Y

W

V

E

W

W

Y

Y

R

W

S

K

T

K

I

I

G

G

G

K

D

E

R

K

A

C

P

P

V

V

V

V

D

D

T
|
T

W
|
W

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

Q

G

I

F

M

M

I

I

T

T

P

P

M

L

P

P

G

G

V

A

T

I

H

E

A

L

K

K

P

A

G

G

S

S

A

A

M

T

R

R

P

P

L

F

P

F

G

G

I

V

V

Q

A

P

D

A

V

L

V

V

N

D

D

N

E

E

G

G

E

H

S

P

V

Q

P

E

D

G

G

A

S

T

G

E

G

G

Y

N

L

L

I

V

I

I

R

T

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D

P

S

W

W

P

P

A

G

M

Q

L

A

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V

L

W

F

G

G

D

D

D

H

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Y

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D

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T

T

Y

Y

W

F

S

S

R

T

W

F

P

K

G

N

V

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

K

R

K

R

D

D

E

E

D

D

G

G

D

Y

I

Y

W

W

V
x
I

L

T

G

G

R

R

V

V

D

D

D

D

V

V

M

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

L

L

S

G

T

T

T

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

P

P

K

K

V
x
I

A

A

E

E

A

A

A

A

V

V

V

V

G

G

A

I

T

P

D

H

E

A

T

I

T

K

G

G

Q

Q

A

A

V

I

C

Y

A

A

F

Y

V

V

I

T

L

L

R

N

E

H

S

G

A

E

G

-

D

E

G

P

G

S

D

P

E

E

I

L

V

Y

A

A

E

E

L

V

R

R

N

N

H

W

V

V

A
x
R

K

K

E

E

I

I

G

G

A
x
P

I

L

A

A

K

T

P

P

R

D

Q

V

V

L

M

H

I

W

V

T

P

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

A

N

G

R

-

E

D

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

S

G

V

V

M

V

active site: T260 (= T257), T412 (= T410), E413 (= E411), N517 (= N515), R522 (= R520), K605 (= K604)
binding (r,r)-2,3-butanediol: W93 (= W91), E140 (= E138), G169 (≠ S167), K266 (= K263), P267 (= P264)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G381), E384 (= E382), P385 (= P383), T408 (= T406), W409 (= W407), W410 (= W408), Q411 (= Q409), T412 (= T410), D496 (= D494), I508 (≠ V506), N517 (= N515), R522 (= R520)
binding coenzyme a: F159 (= F157), G160 (= G158), G161 (= G159), R187 (= R185), S519 (= S517), R580 (≠ A579), P585 (≠ A584)
binding magnesium ion: V533 (= V531), H535 (= H533), I538 (≠ V536)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
51% identity, 95% coverage: 21:631/644 of query aligns to 20:628/637 of 2p2fA

query
sites
2p2fA

E

E

E

T

A

K

Y

Y

A

K

R

Q

A

S

E

I

A

N

D

D

R

P

E

D

G

T

F

F

W

W

A

G

E

E

Q

Q

A

G

E

K

R

I

L

L

D

D

W

W

G

I

Q

T

K

P

W

Y

D

Q

R

K

V

V

L

-

D

-

W

-

D

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

T

E

G

D

G

G

T

T

I

L

N

N

A

L

A

A

V

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

T

Q

A

E

G

-

R

N

G

G

E

D

K

R

V

T

A

A

I

I

H

I

W

W

V

E

G

G

E

D

P

D

E

T

D

S

D

Q

T

S

R

K

D

H

I

I

T

S

Y

Y

A

R

Q

E

L

L

Q

H

D

R

E

D

V

V

N

C

R

R

A

F

A

A

N

N

A

T

L

L

T

L

E

D

L

L

G

G

V

I

A

K

K

K

G

G

D

D

R

V

V

V

A

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

V

V

T

A

M

M

L

L

A

A

C

C

A

A

R

R

L

I

G

G

A

A

P

V

H

H

T

S

V

V

V

I

F
|
F

G

G

G

G

F

F

S

S

A

P

D

E

A

A

L

V

A

A

S

G

R

R

I

I

V

I

D

D

C

S

G

S

A

S

R

R

V

L

V

V

V

I

T

T

A

A

D

D

G

E

G

G

Y

V

R
|
R

R

A

G

G

A

R

P

S

S

I

A

P

L

L

K

K

P

K

A

N

V

V

D

D

E

D

A

A

V

L

A

K

K

N

A

P

D

N

-

V

G

T

L

S

V

V

E

E

H

H

V

V

V

I

V

V

V

L

Q

K

R

R

T

T

G

G

Q

S

E

D

V

I

A

D

F

W

D

Q

E

E

T

G

R

R

D

D

L

L

W

W

W

W

H

R

E

D

L

L

M

I

G

E

R

K

Q

A

S

S

P

P

Q

E

H

H

D

Q

A

P

E

E

L

A

H

M

D

N

A

A

E

E

H

D

P

P

L

L

Y

F

V

I

M

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K