SitesBLAST
Comparing WP_110208085.1 NCBI__GCF_003194585.1:WP_110208085.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6lpiB Crystal structure of ahas holo-enzyme (see paper)
30% identity, 98% coverage: 7:529/533 of query aligns to 7:518/539 of 6lpiB
- active site: I27 (= I27), G29 (= G29), G30 (≠ T30), S31 (≠ H31), I32 (≠ N32), E53 (= E52), C76 (≠ T75), F115 (≠ L116), Q116 (≠ H117), E117 (= E118), K165 (≠ L167), M256 (≠ V255), A283 (≠ W283), V375 (≠ Q382), G401 (= G409), M403 (≠ L411), D428 (= D436), N455 (= N463), A457 (≠ G465), L458 (≠ Y466), L460 (≠ E468), V461 (≠ I469), Q464 (≠ E472)
- binding flavin-adenine dinucleotide: R155 (= R157), G212 (= G212), G213 (= G213), G214 (= G214), T236 (≠ S236), L237 (≠ A237), M238 (≠ N238), L254 (vs. gap), M256 (≠ V255), H257 (= H256), G276 (= G274), A277 (≠ T275), R278 (≠ E276), D280 (≠ A278), R282 (≠ W282), A283 (≠ W283), D300 (= D296), I301 (≠ V297), D319 (= D315), V320 (≠ A316), M380 (≠ Y387), G398 (≠ A406)
- binding magnesium ion: D428 (= D436), N455 (= N463)
- binding thiamine diphosphate: E53 (= E52), C76 (≠ T75), P79 (= P78), G376 (≠ S383), Q377 (≠ M384), H378 (≠ S385), G401 (= G409), M403 (≠ L411), G427 (= G435), D428 (= D436), G429 (= G437), S430 (≠ G438), M433 (≠ F441), N455 (= N463), A457 (≠ G465), L458 (≠ Y466), G459 (= G467), L460 (≠ E468), V461 (≠ I469)
3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
31% identity, 88% coverage: 7:476/533 of query aligns to 12:491/582 of 3ea4A
- active site: Y32 (≠ I27), G34 (= G29), G35 (≠ T30), A36 (≠ H31), S37 (≠ N32), E58 (= E52), T81 (= T75), F120 (≠ L116), Q121 (≠ H117), E122 (= E118), K170 (≠ L167), M265 (≠ V255), V292 (≠ W283), V399 (≠ Q382), G425 (= G409), M427 (≠ L411), D452 (= D436), N479 (= N463), H481 (≠ G465), L482 (≠ Y466), M484 (≠ E468), V485 (≠ I469), W488 (≠ M473)
- binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D281), R291 (≠ W282), W488 (≠ M473)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R157), G221 (= G212), G222 (= G213), G223 (= G214), T245 (≠ S236), L246 (≠ A237), M247 (≠ N238), L263 (vs. gap), G264 (= G254), M265 (≠ V255), H266 (= H256), G285 (= G274), R287 (≠ E276), D289 (≠ A280), R291 (≠ W282), D309 (= D296), I310 (≠ V297), G327 (= G314), D328 (= D315), V329 (≠ A316), M404 (≠ Y387), G422 (≠ A406)
- binding magnesium ion: D452 (= D436), N479 (= N463), H481 (≠ G465)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ Q382), G400 (≠ S383), Q401 (≠ M384), H402 (≠ S385), M427 (≠ L411), G451 (= G435), D452 (= D436), G453 (= G437), S454 (≠ G438), N479 (= N463), H481 (≠ G465), L482 (≠ Y466), G483 (= G467), M484 (≠ E468), V485 (≠ I469)
Sites not aligning to the query:
3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
31% identity, 88% coverage: 7:476/533 of query aligns to 12:491/582 of 3e9yA
- active site: Y32 (≠ I27), G34 (= G29), G35 (≠ T30), A36 (≠ H31), S37 (≠ N32), E58 (= E52), T81 (= T75), F120 (≠ L116), Q121 (≠ H117), E122 (= E118), K170 (≠ L167), M265 (≠ V255), V292 (≠ W283), V399 (≠ Q382), G425 (= G409), M427 (≠ L411), D452 (= D436), N479 (= N463), H481 (≠ G465), L482 (≠ Y466), M484 (≠ E468), V485 (≠ I469), W488 (≠ M473)
- binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D281), R291 (≠ W282), W488 (≠ M473)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R157), G221 (= G212), G222 (= G213), G223 (= G214), T245 (≠ S236), L246 (≠ A237), M247 (≠ N238), L263 (vs. gap), G285 (= G274), R287 (≠ E276), D289 (≠ A280), R291 (≠ W282), D309 (= D296), I310 (≠ V297), G327 (= G314), D328 (= D315), V329 (≠ A316), M404 (≠ Y387), G422 (≠ A406)
- binding magnesium ion: D452 (= D436), N479 (= N463), H481 (≠ G465)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ Q382), G400 (≠ S383), Q401 (≠ M384), H402 (≠ S385), M427 (≠ L411), G451 (= G435), G453 (= G437), S454 (≠ G438), N479 (= N463), H481 (≠ G465), L482 (≠ Y466), G483 (= G467), M484 (≠ E468), V485 (≠ I469)
Sites not aligning to the query:
P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
31% identity, 88% coverage: 7:476/533 of query aligns to 98:577/670 of P17597
- A122 (≠ H31) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- M124 (≠ L33) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
- E144 (= E52) binding thiamine diphosphate
- S186 (= S94) binding FAD
- P197 (= P105) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
- R199 (≠ Q107) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- Q207 (≠ H117) binding thiamine diphosphate
- K220 (≠ A131) binding (R)-imazaquin
- R246 (= R157) binding (R)-imazaquin; binding FAD
- K256 (≠ L167) binding chlorimuron-ethyl
- G308 (= G213) binding FAD
- TL 331:332 (≠ SA 236:237) binding FAD
- C340 (≠ E245) modified: Cysteine sulfinic acid (-SO2H)
- LGMH 349:352 (≠ -GVH 254:256) binding FAD
- GVRF--D 371:375 (≠ GTELAPA 274:280) binding FAD
- DR 376:377 (≠ DW 281:282) binding chlorimuron-ethyl
- DI 395:396 (≠ DV 296:297) binding FAD
- DV 414:415 (≠ DA 315:316) binding FAD
- QH 487:488 (≠ MS 384:385) binding thiamine diphosphate
- GG 508:509 (≠ AG 406:407) binding FAD
- GAM 511:513 (≠ GTL 409:411) binding thiamine diphosphate
- D538 (= D436) binding Mg(2+)
- DGS 538:540 (≠ DGG 436:438) binding thiamine diphosphate
- N565 (= N463) binding Mg(2+)
- NQHLGM 565:570 (≠ NGGYGE 463:468) binding thiamine diphosphate
- H567 (≠ G465) binding Mg(2+)
- W574 (≠ M473) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
Sites not aligning to the query:
- 653 binding chlorimuron-ethyl; S→A: No effect on catalytic activity or sensitivity to herbicides.; S→F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; S→N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; S→T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
9c4rA Acetolactate synthase, chloroplastic (see paper)
31% identity, 88% coverage: 7:476/533 of query aligns to 13:492/582 of 9c4rA
- binding 2-(2-chloroethoxy)-N-[(4-methoxy-6-methylpyrimidin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (≠ V255), R292 (≠ W282), W489 (≠ M473)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ Q382), G401 (≠ S383), Q402 (≠ M384), H403 (≠ S385), G426 (= G409), M428 (≠ L411), G452 (= G435), G454 (= G437), S455 (≠ G438), M458 (≠ F441), N480 (= N463), H482 (≠ G465), L483 (≠ Y466), G484 (= G467), M485 (≠ E468), V486 (≠ I469)
- binding flavin-adenine dinucleotide: R161 (= R157), G222 (= G212), G223 (= G213), G224 (= G214), T246 (≠ S236), L247 (≠ A237), M248 (≠ N238), L264 (vs. gap), M266 (≠ V255), H267 (= H256), G286 (= G274), V287 (≠ T275), R288 (≠ E276), D290 (≠ A280), V293 (≠ W283), D310 (= D296), I311 (≠ V297), D329 (= D315), V330 (≠ A316), M405 (≠ Y387), G423 (≠ A406)
- binding magnesium ion: D453 (= D436), N480 (= N463), H482 (≠ G465)
Sites not aligning to the query:
9c4qA Acetolactate synthase, chloroplastic (see paper)
31% identity, 88% coverage: 7:476/533 of query aligns to 13:492/582 of 9c4qA
- binding 2-(2-fluoroethoxy)-N-[(4-methoxy-6-methylpyrimidin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (≠ V255), D291 (= D281), R292 (≠ W282), W489 (≠ M473)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ Q382), G401 (≠ S383), Q402 (≠ M384), H403 (≠ S385), G426 (= G409), M428 (≠ L411), G452 (= G435), D453 (= D436), G454 (= G437), S455 (≠ G438), N480 (= N463), H482 (≠ G465), L483 (≠ Y466), G484 (= G467), M485 (≠ E468), V486 (≠ I469)
- binding flavin-adenine dinucleotide: R161 (= R157), G222 (= G212), G223 (= G213), G224 (= G214), T246 (≠ S236), L247 (≠ A237), M248 (≠ N238), M263 (≠ A253), L264 (vs. gap), M266 (≠ V255), H267 (= H256), G286 (= G274), V287 (≠ T275), R288 (≠ E276), D290 (≠ A280), R292 (≠ W282), V293 (≠ W283), D310 (= D296), I311 (≠ V297), D329 (= D315), V330 (≠ A316), M405 (≠ Y387), G423 (≠ A406)
- binding magnesium ion: Y381 (vs. gap), D453 (= D436), N480 (= N463), H482 (≠ G465)
- binding oxygen molecule: M458 (≠ F441), Q461 (≠ A444)
Sites not aligning to the query:
9c4pA Acetolactate synthase, chloroplastic (see paper)
31% identity, 88% coverage: 7:476/533 of query aligns to 13:492/582 of 9c4pA
- binding 2-(2-chloroethoxy)-N-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (≠ V255), D291 (= D281), R292 (≠ W282), M485 (≠ E468), W489 (≠ M473)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ Q382), G401 (≠ S383), Q402 (≠ M384), H403 (≠ S385), G426 (= G409), M428 (≠ L411), G452 (= G435), D453 (= D436), G454 (= G437), S455 (≠ G438), M458 (≠ F441), N480 (= N463), H482 (≠ G465), L483 (≠ Y466), G484 (= G467), M485 (≠ E468), V486 (≠ I469)
- binding flavin-adenine dinucleotide: R161 (= R157), G222 (= G212), G223 (= G213), G224 (= G214), T246 (≠ S236), L247 (≠ A237), M248 (≠ N238), M263 (≠ A253), L264 (vs. gap), M266 (≠ V255), H267 (= H256), G286 (= G274), V287 (≠ T275), R288 (≠ E276), D290 (≠ A280), R292 (≠ W282), V293 (≠ W283), D310 (= D296), I311 (≠ V297), D329 (= D315), V330 (≠ A316), M405 (≠ Y387), G423 (≠ A406), G424 (= G407)
- binding magnesium ion: D453 (= D436), N480 (= N463), H482 (≠ G465)
Sites not aligning to the query:
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
31% identity, 88% coverage: 7:476/533 of query aligns to 13:492/582 of 8et4A