SitesBLAST
Comparing WP_110752454.1 NCBI__GCF_003217235.1:WP_110752454.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8cqxA Ribokinase from t.Sp mutant a92g
42% identity, 99% coverage: 1:292/294 of query aligns to 1:294/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N178), T217 (= T214), G219 (= G216), A220 (= A217), G222 (= G219), F250 (= F248), N272 (≠ A270), G275 (≠ A273), A276 (≠ S274), T279 (≠ C277)
- binding magnesium ion: D242 (= D240), T244 (≠ V242), A278 (= A276), S287 (≠ A285)
6znxC Ribokinase from thermus species
40% identity, 99% coverage: 1:292/294 of query aligns to 1:259/265 of 6znxC
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 99% coverage: 2:292/294 of query aligns to 7:301/309 of P0A9J6
1gqtB Activation of ribokinase by monovalent cations (see paper)
40% identity, 99% coverage: 2:292/294 of query aligns to 6:300/307 of 1gqtB
- active site: A251 (≠ G243), A252 (= A244), G253 (= G245), D254 (= D246)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N178), T222 (= T214), G224 (= G216), S225 (≠ A217), A252 (= A244), G253 (= G245), H278 (≠ A270), A281 (= A273)
- binding cesium ion: D248 (= D240), I250 (≠ V242), A284 (= A276), R287 (≠ K279), S293 (≠ A285)
- binding alpha-D-ribofuranose: N13 (= N9), D15 (= D11), G41 (= G37), N45 (= N41), E142 (= E134), D254 (= D246)
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
40% identity, 99% coverage: 2:292/294 of query aligns to 4:298/305 of 1rk2A
- active site: A249 (≠ G243), A250 (= A244), G251 (= G245), D252 (= D246)
- binding adenosine-5'-diphosphate: T220 (= T214), G222 (= G216), S223 (≠ A217), A250 (= A244), G251 (= G245), H276 (≠ A270), A279 (= A273)
- binding tetrafluoroaluminate ion: G213 (= G208), R215 (= R209)
- binding magnesium ion: D246 (= D240), A282 (= A276), R285 (≠ K279), S291 (≠ A285)
- binding alpha-D-ribofuranose: N11 (= N9), D13 (= D11), G38 (= G36), G39 (= G37), K40 (= K38), N43 (= N41), E140 (= E134), D252 (= D246)
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
35% identity, 99% coverage: 2:292/294 of query aligns to 4:298/306 of 4xckA
- active site: A249 (≠ G243), A250 (= A244), G251 (= G245), D252 (= D246)
- binding adenosine-5'-diphosphate: T220 (= T214), G222 (= G216), S223 (≠ A217), V242 (= V236), T247 (= T241), A250 (= A244), F254 (= F248), H276 (≠ A270), A279 (= A273), V283 (≠ C277)
- binding alpha-D-ribofuranose: N11 (= N9), D13 (= D11), G39 (= G37), K40 (= K38), N43 (= N41), A95 (= A92), I107 (= I104), I109 (≠ V106), E140 (= E134), T248 (≠ V242), D252 (= D246)
6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
35% identity, 99% coverage: 2:293/294 of query aligns to 5:305/311 of 6wk0B
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N178), T222 (= T214), G224 (= G216), A225 (= A217), G227 (= G219), T243 (≠ A233), V246 (= V236), A254 (= A244), G255 (= G245), N282 (≠ A270), A285 (= A273), A286 (≠ S274), V289 (≠ C277)
- binding alpha-D-ribofuranose: D14 (= D11), G40 (= G37), K41 (= K38), N44 (= N41), A96 (= A92), E141 (= E134), D256 (= D246)
5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
35% identity, 99% coverage: 2:293/294 of query aligns to 5:305/317 of 5c41A
- active site: G253 (= G243), A254 (= A244), G255 (= G245), D256 (= D246)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N178), T222 (= T214), G224 (= G216), A225 (= A217), G227 (= G219), V246 (= V236), G255 (= G245), N282 (≠ A270), A285 (= A273), A286 (≠ S274)
6wjzA Crystal structure of human ribokinase in complex with ampcp
35% identity, 99% coverage: 2:293/294 of query aligns to 5:305/315 of 6wjzA
- binding phosphomethylphosphonic acid adenosyl ester: N186 (= N178), T222 (= T214), G224 (= G216), A225 (= A217), G227 (= G219), T243 (≠ A233), V246 (= V236), A254 (= A244), G255 (= G245), N282 (≠ A270), A285 (= A273), A286 (≠ S274), V289 (≠ C277)
5c3yA Structure of human ribokinase crystallized with amppnp
35% identity, 99% coverage: 2:293/294 of query aligns to 4:304/306 of 5c3yA
- active site: G252 (= G243), A253 (= A244), G254 (= G245), D255 (= D246)
- binding amp phosphoramidate: T221 (= T214), G223 (= G216), V245 (= V236), T250 (= T241), G254 (= G245), N281 (≠ A270), A284 (= A273), A285 (≠ S274)
2fv7A Crystal structure of human ribokinase
35% identity, 99% coverage: 2:293/294 of query aligns to 4:304/308 of 2fv7A
- active site: G252 (= G243), A253 (= A244), G254 (= G245), D255 (= D246)
- binding adenosine-5'-diphosphate: N185 (= N178), T221 (= T214), G223 (= G216), G226 (= G219), T242 (≠ A233), V245 (= V236), A253 (= A244), G254 (= G245), N281 (≠ A270), A284 (= A273), A285 (≠ S274), V288 (≠ C277)
6a8cA Ribokinase from leishmania donovani with adp (see paper)
33% identity, 99% coverage: 2:293/294 of query aligns to 16:323/327 of 6a8cA
- binding adenosine-5'-diphosphate: G245 (= G216), A246 (= A217), T271 (= T241), A274 (= A244), G275 (= G245), N300 (≠ A270), A303 (= A273)
- binding glycerol: D25 (= D11), S42 (≠ G28), S44 (≠ D30), G50 (= G36), G51 (= G37), N55 (= N41)
6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
33% identity, 99% coverage: 2:293/294 of query aligns to 16:323/327 of 6a8bA
- binding phosphomethylphosphonic acid adenylate ester: G245 (= G216), A246 (= A217), T271 (= T241), A274 (= A244), G275 (= G245), N300 (≠ A270), A303 (= A273), V307 (≠ C277)
- binding glycerol: D25 (= D11), G50 (= G36), G51 (= G37), N55 (= N41), N157 (≠ H133), I159 (= I135), E190 (≠ W161)
6a8aA Ribokinase from leishmania donovani with atp (see paper)
33% identity, 99% coverage: 2:293/294 of query aligns to 16:323/327 of 6a8aA
- binding adenosine-5'-triphosphate: N207 (= N178), T243 (= T214), G245 (= G216), A246 (= A217), G248 (= G219), T271 (= T241), G273 (= G243), A274 (= A244), G275 (= G245), N300 (≠ A270), A303 (= A273), V307 (≠ C277)
- binding glycerol: D25 (= D11), G50 (= G36), G51 (= G37), N55 (= N41)
5byfA Crystal structure of human ribokinase in complex with amp
35% identity, 98% coverage: 6:293/294 of query aligns to 10:306/313 of 5byfA
Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
35% identity, 98% coverage: 6:293/294 of query aligns to 22:318/322 of Q9H477
P0DX97 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhi (see paper)
33% identity, 99% coverage: 2:292/294 of query aligns to 3:298/306 of P0DX97
- M10 (≠ N9) Important for substrate specificity; mutation to N: 2.5-fold decrease in Vmax for deoxyribokinase activity and 70-fold increase in KM for deoxyribose. 2-fold increase in KM for ribose.
6xk2A Crystal structure of ribokinase from cryptococcus neoformans var. Grubii serotype a in complex with adp
36% identity, 97% coverage: 4:287/294 of query aligns to 8:311/320 of 6xk2A
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
36% identity, 97% coverage: 2:287/294 of query aligns to 5:300/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N178), K225 (≠ T214), G227 (= G216), I246 (≠ M231), A248 (= A233), A257 (= A244), G258 (= G245), F261 (= F248), A286 (= A273), S287 (= S274)
- binding alpha-D-ribofuranose: N12 (= N9), D14 (= D11), G40 (= G37), K41 (= K38), N44 (= N41), E144 (= E134), D259 (= D246)
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 97% coverage: 2:287/294 of query aligns to 71:366/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
Query Sequence
>WP_110752454.1 NCBI__GCF_003217235.1:WP_110752454.1
MITVIGSINLDLIARTMHLPKPGETVSGSDFQSAPGGKGANQALAAARAGARVRMVGAIG
NDAFGGESMALLDEGGVDLSLVRKAEVATGIALITVEASGENVIVVVPGANGTVSLGDVK
AAGLSGGHLLLQHEIPLATVGAALKAARENGTISILNIAPWRDEAADLLPLADIVIANET
EFDLAASRLKLEGADRESRMADFAAKTGRTVIVTLGADGLCAATPQEAFAMPAYPVKPVD
TVGAGDTFCGYLAAGLDMGLALKAACRQAAIAASLACLKPGAQPAIPFADELPT
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory