Comparing WP_110805530.1 NCBI__GCF_003217355.1:WP_110805530.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 13 hits to proteins with known functional sites (download)
3klcB Crystal structure of hyperthermophilic nitrilase (see paper)
32% identity, 65% coverage: 89:283/300 of query aligns to 79:257/261 of 3klcB
Sites not aligning to the query:
3klcA Crystal structure of hyperthermophilic nitrilase (see paper)
32% identity, 65% coverage: 89:283/300 of query aligns to 79:257/261 of 3klcA
Sites not aligning to the query:
7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide (see paper)
39% identity, 44% coverage: 84:214/300 of query aligns to 76:200/263 of 7ovgA
Sites not aligning to the query:
6ypaB The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
39% identity, 44% coverage: 84:214/300 of query aligns to 82:206/269 of 6ypaB
Sites not aligning to the query:
Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see paper)
32% identity, 65% coverage: 89:283/300 of query aligns to 80:258/262 of Q9UYV8
Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 2 papers)
25% identity, 64% coverage: 84:275/300 of query aligns to 72:261/276 of Q9NQR4
Sites not aligning to the query:
P46011 Bifunctional nitrilase/nitrile hydratase NIT4; Cyanoalanine nitrilase; Nitrilase 4; EC 3.5.5.1; EC 3.5.5.4; EC 4.2.1.65 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 67% coverage: 100:300/300 of query aligns to 139:341/355 of P46011
4hg5A Structural insights into yeast nit2: wild-type yeast nit2 in complex with oxaloacetate (see paper)
34% identity, 28% coverage: 131:215/300 of query aligns to 139:224/304 of 4hg5A
Sites not aligning to the query:
4hg3A Structural insights into yeast nit2: wild-type yeast nit2 in complex with alpha-ketoglutarate (see paper)
34% identity, 28% coverage: 131:215/300 of query aligns to 139:224/304 of 4hg3A
Sites not aligning to the query:
P47016 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
34% identity, 28% coverage: 131:215/300 of query aligns to 142:227/307 of P47016
Q94JV5 Deaminated glutathione amidase, chloroplastic/cytosolic; dGSH amidase; Nitrilase-like protein 2; Protein nitrilase 1 homolog; AtNit1; Protein Nit1 homolog; EC 3.5.1.128 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 70% coverage: 5:214/300 of query aligns to 36:246/307 of Q94JV5
Sites not aligning to the query:
Q9UBR1 Beta-ureidopropionase; BUP-1; Beta-alanine synthase; N-carbamoyl-beta-alanine amidohydrolase; EC 3.5.1.6 from Homo sapiens (Human) (see 4 papers)
24% identity, 83% coverage: 16:263/300 of query aligns to 90:345/384 of Q9UBR1
Sites not aligning to the query:
4hgdA Structural insights into yeast nit2: c169s mutant of yeast nit2 in complex with an endogenous peptide-like ligand (see paper)
28% identity, 44% coverage: 83:215/300 of query aligns to 81:222/299 of 4hgdA
Sites not aligning to the query:
>WP_110805530.1 NCBI__GCF_003217355.1:WP_110805530.1
MRHVTIAAAAYPLDWAEDWAAYEAKTRAWVQMAALKQADLLVFPEYGAMELAALGGAAVA
ADLTLAVEEVLRHRAAMERHFEGLAIEFGLYILAPSGPAQDGLELRNRAVLFGPSGRIGH
QDKAIMTRFEREDWNIGPGRGLTLFDTGIGKIGIVICYDSEFPLLSRGLCEAGAEILLAP
SCTDAAAGYNRVRIGSQARALENQCVTVQAPVVGLAPWCPAVDENHGAAGIYGPPDEGWP
DDGVIAKGVYDEPGWVVATVDLDRVTHSRRDGSVLLFQHWSEQPLQAVQIRTVAESPRKS
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory