SitesBLAST

P21439 Phosphatidylcholine translocator ABCB4; ATP-binding cassette sub-family B member 4; Multidrug resistance protein 3; P-glycoprotein 3; EC 7.6.2.1 from Homo sapiens (Human) (see 22 papers)
36% identity, 42% coverage: 10:223/511 of query aligns to 389:608/1286 of P21439

query
sites
P21439

S

S

I

I

R

K

G

G

G

N

V

L

K

E

V

F

-

N

-

D

-

V

-

H

-

F

-

S

Y
|
Y

P

P

-

S

-
x
R

-

A

G

N

T

V

R

K

A

I

L

L

K

K

G

G

V

L

D

N

F

L

D

K

L

V

R

Q

M

S

G

G

A

Q

V

T

N

V

V

A

L

L

V

V

G

G

E

S

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

T

M

V

K

Q

L

L

I

I

A

Q

G

R

V

L

E

Y

D

D

M

P

T

D

E
|
E

G

G

T

T

I

I

T

N

M

I

D

D

G

G

R

Q

E
x
D

M

I

R

R

F

-

R

N

T

F

K

N

A

V

D

N

A

Y

V

L

A

R

A

E

G

I

I

I

G

G

I

V

V

V

F

S

Q
|
Q

E

E

L
x
P

N

V

L
|
L

F

F

P

S

N

T

L

-

S

T

V

I

A

A

E

E

N

N

I

I

F

C

I

Y

G

G

H

-

E

-

T

-

R

R

G

G

G

N

I

V

D

T

I

M

D

D

-

E

-

I

I

K

E

K

A

A

H

V

R

K

E

E

A

A

T

N

-
x
A

R

Y

Q

E

L

F

M

I

E

M

R

K

L

L

E

P

Q

Q

N

K

I

F

H

-

P

-

D

D

T

T

P

L

L

V

G

G

-
x
E

-

R

-

G

-

A

N

Q

L

L

R

S

I
x
G

G
|
G

Q

Q

Q

K

Q

Q

I

R

V
x
I

E

A

I

I

A

A

K

R

A
|
A

L

L

A

V

Q

R

N

N

A

P

R

K

I

I

L

L

I

L

L

L

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

-

D

-

T

-

E

S

S

A

E

A

A

E

E

V

V

E

Q

V

A

L

-

F

-

R

-

V

A

I

L

D

D

E

K

L

-

T

A

A

R

Q

E

G

G

V

R

G

T

I

T

V

I

Y

V

I

I

S

A

H
|
H

R
|
R

L

L

E

S

E

T

L

-

I

V

R

R

V

N

G

A

D

D

Y

V

I

I

T

A

V

G

L

F

R

E

D

D

G

G

V

V

I

I

Y403 (= Y18) to H: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol; dbSNP:rs121918443
R406 (vs. gap) binding ATP; to Q: found in patients with cholangitis; uncertain significance; dbSNP:rs763807769
GCGKST 432:437 (≠ GAGKST 44:49) binding ATP
K435 (= K47) mutation to M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
E450 (= E62) to G: in dbSNP:rs1189003716
D459 (≠ E71) to H: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression
Q477 (= Q90) binding ATP
P479 (≠ L92) to L: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs748657435
L481 (= L94) to R: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol
A511 (vs. gap) to T: in PFIC3 and GBD1; dbSNP:rs1257887155
E528 (vs. gap) to D: in GBD1; uncertain significance; moderate decrease of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs8187797
G535 (≠ I148) to D: in PFIC3; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs1810849139
G536 (= G149) binding ATP; to R: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location
I541 (≠ V154) to F: in PFIC3 and GBD1; dbSNP:rs66904256
A546 (= A159) to D: in ICP3; disruption of protein trafficking with subsequent lack of functional protein at the cell surface; dbSNP:rs121918441
E558 (= E171) mutation to Q: Loss of floppase activity. Strongly reduce the ATPase activity.
H589 (= H203) to T: in GBD1; requires 2 nucleotide substitutions
R590 (= R204) to Q: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs45575636

Sites not aligning to the query:

34 modified: Phosphothreonine; T → M: in GBD1; reduces efflux activity for PC in a phosphorylation-dependent manner; dbSNP:rs142794414; T→D: Does not inhibit efflux activity for PC.
44 T→A: Reduces efflux activity for PC. Does not alter apical membrane location.
47 R → G: in GBD1; partly retained intracellularly; reduces efflux activity for PC in a phosphorylation-dependent manner; R → Q: found in patients with cholangitis; uncertain significance; dbSNP:rs372685632
49 S→A: Reduces efflux activity for PC. Does not alter apical membrane location.
68 G → R: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression; dbSNP:rs1343667900
73 L → V: in PFIC3 and GBD1; dbSNP:rs8187788
87 natural variant: D -> E
95 P → S: in dbSNP:rs377268767
175 T → A: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs58238559
201 T → M: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs753318087
238 L → V: in dbSNP:rs45596335
263 I → V: in dbSNP:rs45547936
286 A → V: in PFIC3 and GBD1; does not alter plasma membrane location; inhibits efflux activity for PC; dbSNP:rs765478923
320 S → F: in ICP3, GBD1 and PFIC3; uncertain significance; does not alter plasma membrane location; does not inhibit efflux activity for PC; dbSNP:rs72552778
367 I → V: in dbSNP:rs1168923653
651 T → N: in dbSNP:rs45476795
652 R → G: in dbSNP:rs2230028
726 P → L: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs141677867
742 natural variant: G -> S
764 I → L: in a heterozygous patient with risperidone-induced cholestasis
775 T → M: found in patients with cholangitis; uncertain significance; dbSNP:rs148052192
788 R → Q: in GBD1; benign; dbSNP:rs8187801
934 A → T: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs61730509
953 A→D: Accumulates predominantly in intracellular compartments with only a small fraction at the plasma membrane and inhibits partially the efflux activity for PC.
964 V → T: found in patients with cholangitis; uncertain significance; requires 2 nucleotide substitutions
978 S → P: in PFIC3; alters efflux activity for PC; dbSNP:rs1051861187
985 V→M: Significantly reduces phosphatidylcholine floppase activity; when associated with Q-989 and V-990.
989 H→Q: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and V-990.
990 A→V: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and Q-989.
1046 binding ATP
1071:1077 binding ATP
1075 K→M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
1082 L → Q: in a heterozygous patient with amoxicillin/clavulanic acid-induced cholestasis; dbSNP:rs1214110864
1124 binding ATP
1125 E → K: in PFIC3; alters efflux activity for PC
1168 P → S: in GBD1; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs121918442
1183 S → L: in GBD1; severely reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
1184:1186 binding ATP
1185 G → S: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
30% identity, 40% coverage: 18:221/511 of query aligns to 13:218/344 of 6cvlD

query
sites
6cvlD

Y

H

P
x
Q

G

G

T

T

R

R

-

T

-
x
I

-

Q

A

A

L

L

K

N

G

N

V

V

D

S

F

L

D

H

L

V

R

P

M

A

G

G

A

Q

V

I

N

Y

V

G

L

V

V

I

G

G

E

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

L

C

I

V

A

N

G

L

V

L

E

E

D

R

M

P

T

T

E

E

G

G

T

S

I

V

T

L

M

V

D

D

G

G

R

Q

E

E

M

L

R

T

F

T

R

L

T

S

K

E

A

S

D

E

A

L

V

T

A

K

A

A

-

R

G

Q

I

I

G

G

I

M

V

I

F

F

Q

Q

E

H

L

F

N

N

L

L

F

L

P

S

N

S

L

R

S

T

V

V

A

F

E

G

N

N

I

V

F

A

I

L

G

-

H

-

E

-

T

-

R

-

G

-

G

P

I

L

D

E

I

L

D

D

I

N

E

T

A

P

H

K

R

D

E

E

A

V

T

K

R

R

Q

R

L

V

M

T

E

E

R

L

L

L

E

S

Q

L

-

V

-

G

-

L

-

G

N

D

I

K

H

H

P

D

D

S

T

Y

P

P

L

-

G

S

N
|
N

L

L

R
x
S

I

G

G

G

Q
|
Q

Q

K

Q

Q

I

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

A

A

Q

S

N

N

A

P

R

K

I

V

L

L

I

L

L

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

S

D

A

P

A

A

E

T

V

T

E

R

V

S

L

I

F

L

R

E

V

L

I

L

D

K

E

D

L

I

T

N

A

R

Q

R

-

L

G

G

V

L

G

T

I

I

V

L

Y

L

I

I

S

T

H
|
H

R

E

L

M

E

D

E

V

L

V

I

K

R

R

V

I

G

C

D

D

Y

C

I

V

T

A

V

V

L

I

R

S

D

N

G

G

binding phosphothiophosphoric acid-adenylate ester: Q14 (≠ P19), I19 (vs. gap), S41 (≠ N43), G42 (= G44), A43 (= A45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49), N141 (= N145), S143 (≠ R147), Q146 (= Q150), H200 (= H203)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
28% identity, 41% coverage: 15:223/511 of query aligns to 11:228/254 of 1g6hA

query
sites
1g6hA

V

V

K

K

V

Y

Y
x
F

P

G

G

E

T
x
F

R

K

A

A

L

L

K

D

G

G

V

V

D

S

F

I

D

S

L

V

R

N

M

K

G

G

A

D

V

V

N

T

V

L

L

I

V

I

G

G

E

P

N
|
N

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

N

L

V

I

I

A

T

G

G

V

F

E

L

D

K

M

A

T

D

E

E

G

G

T

R

I

V

T

Y

M

F

D

E

G

N

R

K

E

D

M

I

R

T

F

N

R

K

T

E

K

P

A

A

D

E

A

L

V

Y

A

H

A

Y

G

G

I

I

G

V

I

R

V

T

F

F

Q

Q

E

T

L

P

N

Q

L

P

F

L

P

K

N

E

L

M

S

T

V

V

A

L

E

E

N

N

I

L

F

L

I

I

G

G

H

-

E

E

T

I

T

C

R

P

G

G

G

E

I

S

D

P

I

L

D

N

I

S

E

L

A

F

H

Y

R

K

-

K

-

W

-

I

-

P

-

K

-

E

-

M

-

V

E

E

A

K

T

A

R

F

Q

K

L

I

M

L

E

E

R

F

L

L

E

K

Q

L

N

S

I

H

H

L

P

Y

D

D

T

R

P

K

L

A

G

G

N

E

L

L

R

S

I

G

G

G

Q

Q

Q

M

Q

K

I

L

V

V

E

E

I

I

A

G

K

R

A

A

L

L

A

M

Q

T

N

N

A

P

R

K

I

M

L

I

I

V

L

M

D

D

E

E

P

P

T

I

S

A

A

G

L

V

S

A

A

P

A

G

E

L

V

A

E

H

V

D

L

I

F

F

R

N

V

H

I

V

D

L

E

E

L

L

T

K

A

A

Q

K

G

G

V

I

G

T

I

F

V

L

Y

I

I

I

S

E

H

H

R

R

L

L

E

D

E

I

L

V

I

L

R

N

V

Y

G

I

D

D

Y

H

I

L

T

Y

V

V

L

M

R

F

D

N

G

G

V

Q

I

I

binding adenosine-5'-diphosphate: F14 (≠ Y18), F17 (≠ T21), N39 (= N43), G40 (= G44), G42 (= G46), K43 (= K47), S44 (= S48), T45 (= T49)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
30% identity, 40% coverage: 18:221/511 of query aligns to 13:218/344 of 3tuzC

query
sites
3tuzC

Y

H

P
x
Q

G

G

T

T

R

R

-

T

-

I

-

Q

A

A

L

L

K

N

G

N

V

V

D

S

F

L

D

H

L

V

R

P

M

A

G

G

A

Q

V

I

N

Y

V

G

L

V

V

I

G

G

E

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

L

C

I

V

A

N

G

L

V

L

E

E

D

R

M

P

T

T

E

E

G

G

T

S

I

V

T

L

M

V

D

D

G

G

R

Q

E

E

M

L

R

T

F

T

R

L

T

S

K

E

A

S

D

E

A

L

V

T

A

K

A

A

-

R

G

Q

I

I

G

G

I

M

V

I

F

F

Q

Q

E

H

L

F

N

N

L

L

F

L

P

S

N

S

L

R

S

T

V

V

A

F

E

G

N

N

I

V

F

A

I

L

G

-

H

-

E

-

T

-

R

-

G

-

G

P

I

L

D

E

I

L

D

D

I

N

E

T

A

P

H

K

R

D

E

E

A

V

T

K

R

R

Q

R

L

V

M

T

E

E

R

L

L

L

E

S

Q

L

-

V

-

G

-

L

-

G

N

D

I

K

H

H

P

D

D

S

T

Y

P

P

L

-

G

S

N

N

L

L

R

S

I

G

G

G

Q

Q

Q

K

Q

Q

I

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

A

A

Q

S

N

N

A

P

R

K

I

V

L

L

I

L

L

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

S

D

A

P

A

A

E

T

V

T

E

R

V

S

L

I

F

L

R

E

V

L

I

L

D

K

E

D

L

I

T

N

A

R

Q

R

-

L

G

G

V

L

G

T

I

I

V

L

Y

L

I

I

S

T

H

H

R

E

L

M

E

D

E

V

L

V

I

K

R

R

V

I

G

C

D

D

Y

C

I

V

T

A

V

V

L

I

R

S

D

N

G

G

binding adenosine-5'-diphosphate: Q14 (≠ P19), G42 (= G44), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
30% identity, 40% coverage: 18:221/511 of query aligns to 13:218/344 of 3tuiC

query
sites
3tuiC

Y

H

P

Q

G

G

T

T

R

R

-

T

-

I

-

Q

A

A

L

L

K

N

G

N

V

V

D

S

F

L

D

H

L

V

R

P

M

A

G

G

A

Q

V

I

N

Y

V

G

L

V

V

I

G

G

E

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

L

C

I

V

A

N

G

L

V

L

E

E

D

R

M

P

T

T

E

E

G

G

T

S

I

V

T

L

M

V

D

D

G

G

R

Q

E

E

M

L

R

T

F

T

R

L

T

S

K

E

A

S

D

E

A

L

V

T

A

K

A

A

-

R

G

Q

I

I

G

G

I

M

V

I

F

F

Q

Q

E

H

L

F

N

N

L

L

F

L

P

S

N

S

L

R

S

T

V

V

A

F

E

G

N

N

I

V

F

A

I

L

G

-

H

-

E

-

T

-

R

-

G

-

G

P

I

L

D

E

I

L

D

D

I

N

E

T

A

P

H

K

R

D

E

E

A

V

T

K

R

R

Q

R

L

V

M

T

E

E

R

L

L

L

E

S

Q

L

-

V

-

G

-

L

-

G

N

D

I

K

H

H

P

D

D

S

T

Y

P

P

L

-

G

S

N

N

L

L

R

S

I

G

G

G

Q

Q

Q

K

Q

Q

I

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

A

A

Q

S

N

N

A

P

R

K

I

V

L

L

I

L

L

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

S

D

A

P

A

A

E

T

V

T

E

R

V

S

L

I

F

L

R

E

V

L

I

L

D

K

E

D

L

I

T

N

A

R

Q

R

-

L

G

G

V

L

G

T

I

I

V

L

Y

L

I

I

S

T

H

H

R

E

L

M

E

D

E

V

L

V

I

K

R

R

V

I

G

C

D

D

Y

C

I

V

T

A

V

V

L

I

R

S

D

N

G

G

binding adenosine-5'-diphosphate: G42 (= G44), A43 (= A45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
28% identity, 41% coverage: 15:223/511 of query aligns to 11:228/253 of 1g9xB

query
sites
1g9xB

V

V

K

K

V

Y

Y
x
F

P

G

G

E

T
x
F

R

K

A

A

L

L

K

D

G

G

V

V

D

S

F

I

D

S

L

V

R

C

M

K

G

G

A

D

V

V

N

T

V

L

L

I

V

I

G

G

E

P

N

N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

N

L

V

I

I

A

T

G

G

V

F

E

L

D

K

M

A

T

D

E

E

G

G

T

R

I

V

T

Y

M

F

D

E

G

N

R

K

E

D

M

I

R

T

F

N

R

K

T

E

K

P

A

A

D

E

A

L

V

Y

A

H

A

Y

G

G

I

I

G

V

I

R

V

T

F

F

Q

Q

E

T

L

P

N

Q

L

P

F

L

P

K

N

E

L

M

S

T

V

V

A

L

E

E

N

N

I

L

F

L

I

I

G

G

H

-

E

E

T

I

T

N

R

P

G

G

G

E

I

S

D

P

I

L

D

N

I

S

E

L

A

F

H

Y

R

K

-

K

-

W

-

I

-

P

-

K

-

E

-

M

-

V

E

E

A

K

T

A

R

F

Q

K

L

I

M

L

E

E

R

F

L

L

E

K

Q

L

N

S

I

H

H

L

P

Y

D

D

T

R

P

K

L

A

G

G

N

E

L

L

R

S

I

G

G

G

Q

Q

Q

M

Q

K

I

L

V

V

E

E

I

I

A

G

K

R

A

A

L

L

A

M

Q

T

N

N

A

P

R

K

I

M

L

I

I

V

L

M

D

D

E
|
E

P

P

T

I

S

A

A

G

L

V

S

A

A

P

A

G

E

L

V

A

E

H

V

D

L

I

F

F

R

N

V

H

I

V

D

L

E

E

L

L

T

K

A

A

Q

K

G

G

V

I

G

T

I

F

V

L

Y

I

I

I

S

E

H

H

R

R

L

L

E

D

E

I

L

V

I

L

R

N

V

Y

G

I

D

D

Y

H

I

L

T

Y

V

V

L

M

R

F

D

N

G

G

V

Q

I

I

binding adenosine-5'-diphosphate: F14 (≠ Y18), F17 (≠ T21), G40 (= G44), S41 (≠ A45), G42 (= G46), K43 (= K47), S44 (= S48), T45 (= T49)
binding magnesium ion: S44 (= S48), E176 (= E171)

6s7pA Nucleotide bound abcb4 (see paper)
36% identity, 42% coverage: 10:223/511 of query aligns to 328:547/1128 of 6s7pA

query
sites
6s7pA

S

S

I

I

R

K

G

G

G

N

V

L

K

E

V

F

-

N

-

D

-

V

-

H

-

F

-

S

Y
|
Y

P

P

-
x
S

-
x
R

-

A

G

N

T

V

R

K

A

I

L

L

K

K

G

G

V

L

D

N

F

L

D

K

L

V

R

Q

M

S

G

G

A

Q

V

T

N

V

V

A

L

L

V

V

G

G

E

S

N

S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T

T

L

T

M

V

K

Q

L

L

I

I

A

Q

G

R

V

L

E

Y

D

D

M

P

T

D

E

E

G

G

T

T

I

I

T

N

M

I

D

D

G

G

R

Q

E

D

M

I

R

R

F

-

R

N

T

F

K

N

A

V

D

N

A

Y

V

L

A

R

A

E

G

I

I

I

G

G

I

V

V

V

F

S

Q
|
Q

E

E

L

P

N

V

L

L

F

F

P

S

N

T

L

-

S

T

V

I

A

A

E

E

N

N

I

I

F

C

I

Y

G

G

H

-

E

-

T

-

R

R

G

G

G

N

I

V

D

T

I

M

D

D

-

E

-

I

I

K

E

K

A

A

H

V

R

K

E

E

A

A

T

N

-

A

R

Y

Q

E

L

F

M

I

E

M

R

K

L

L

E

P

Q

Q

N

K

I

F

H

-

P

-

D

D

T

T

P

L

L

V

G

G

-

E

-

R

-

G

-

A

N
x
Q

L

L

R
x
S

I

G

G
|
G

Q
|
Q

Q

K

Q

Q

I

R

V

I

E

A

I

I

A

A

K

R

A

A

L

L

A

V

Q

R

N

N

A

P

R

K

I

I

L

L

I

L

L

L

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

-

D

-

T

-

E

S

S

A

E

A

A

E

E

V

V

E

Q

V

A

L

-

F

-

R

-

V

A

I

L

D

D

E

K

L

-

T

A

A

R

Q

E

G

G

V

R

G

T

I

T

V

I

Y

V

I

I

S

A

H

H

R

R

L

L

E

S

E

T

L

-

I

V

R

R

V

N

G

A

D

D

Y

V

I

I

T

A

V

G

L

F

R

E

D

D

G

G

V

V

I

I

binding adenosine-5'-triphosphate: Y342 (= Y18), S344 (vs. gap), R345 (vs. gap), G371 (= G44), G373 (= G46), K374 (= K47), S375 (= S48), Q416 (= Q90), Q471 (≠ N145), S473 (≠ R147), G475 (= G149), Q476 (= Q150)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 920, 922, 923, 948, 949, 951, 952, 953, 954, 994, 1033, 1053, 1054, 1056
binding cholesterol: 25, 32, 35, 65, 824, 834, 834, 838
binding magnesium ion: 953, 994

7niwA Nanodisc reconstituted human abcb4 in complex with 4b1-fab (posaconazole-bound, inward-open conformation) (see paper)
36% identity, 42% coverage: 10:223/511 of query aligns to 338:557/1140 of 7niwA

query
sites
7niwA

S

S

I

I

R

K

G

G

G

N

V

L

K

E

V

F

-

N

-

D

-

V

-

H

-

F

-

S

Y

Y

P

P

-

S

-

R

-

A

G

N

T

V

R

K

A

I

L

L

K

K

G

G

V

L

D

N

F

L

D

K

L

V

R

Q

M

S

G

G

A

Q

V

T

N

V

V

A

L

L

V

V

G

G

E

S

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

T

M

V

K

Q

L

L

I

I

A

Q

G

R

V

L

E

Y

D

D

M

P

T

D

E

E

G

G

T

T

I

I

T

N

M

I

D

D

G

G

R

Q

E

D

M

I

R

R

F

-

R

N

T

F

K

N

A

V

D

N

A

Y

V

L

A

R

A

E

G

I

I

I

G

G

I

V

V

V

F

S

Q

Q

E

E

L

P

N

V

L

L

F

F

P

S

N

T

L

-

S

T

V

I

A

A

E

E

N

N

I

I

F

C

I

Y

G

G

H

-

E

-

T

-

R

R

G

G

G

N

I

V

D

T

I

M

D

D

-

E

-

I

I

K

E

K

A

A

H

V

R

K

E

E

A

A

T

N

-

A

R

Y

Q

E

L

F

M

I

E

M

R

K

L

L

E

P

Q

Q

N

K

I

F

H

-

P

-

D

D

T

T

P

L

L

V

G

G

-

E

-

R

-

G

-

A

N

Q

L

L

R

S

I

G

G

G

Q

Q

Q

K

Q

Q

I

R

V

I

E

A

I

I

A

A

K

R

A

A

L

L

A

V

Q

R

N

N

A

P

R

K

I

I

L

L

I

L

L

L

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

-

D

-

T

-

E

S

S

A

E

A

A

E

E

V

V

E

Q

V

A

L

-

F

-

R

-

V

A

I

L

D

D

E

K

L

-

T

A

A

R

Q

E

G

G

V

R

G

T

I

T

V

I

Y

V

I

I

S

A

H

H

R

R

L

L

E

S

E

T

L

-

I

V

R

R

V

N

G

A

D

D

Y

V

I

I

T

A

V

G

L

F

R

E

D

D

G

G

V

V

I

I

Sites not aligning to the query:

binding cholesterol: 70, 152, 160, 266, 641, 645, 648, 839
binding 1,2-dilinoleoyl-sn-glycero-3-phosphocholine: 173, 183, 250, 254, 257, 261, 286, 294, 298, 617, 882
binding posaconazole: 250, 291, 294, 658, 661, 726, 841, 871, 874, 878, 882

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 43% coverage: 4:223/511 of query aligns to 9:226/378 of P69874

query
sites
P69874

K

K

H

Q

P

P

I

S

G

S

L

L

S

S

-

P

-

L

-

V

-

Q

I

L

R

A

G

G

G

I

V

R

K

K

V
x
C

Y
x
F

P

D

G

G

T

K

R

E

A

V

L

I

K

P

G

Q

V

L

D

D

F

L

D

T

L

I

R

N

M

N

G

G

A

E

V
x
F

N

L

V

T

L

L

V

L

G

G

E

P

N

S

G

G

A
x
C

G

G

K

K

S

T

T

T

L

V

M
x
L

K

R

L

L

I

I

A

A

G

G

V

L

E

E

D

T

M

V

T

D

E

S

G

G

T

R

I

I

T

M

M
x
L

D

D

G

N

R

E

E

D

M

I

R

-

F

-

R

-

T

T

K

H

A

V

D

P

A

A

V

E

A

N

A

R

G

Y

I

V

G

N

I

T

V

V

F

F

Q

Q

E

S

L

Y

N

A

L

L

F

F

P

P

N

H

L

M

S

T

V

V

A

F

E

E

N

N

I

V

F

A

I

F

G

-

H

-

E

-

T

-

R

-

G

-

G

G

I

L

D

R

I

M

D

Q

I

K

E

T

A

P

H

A

R

A

E

E

A

I

T

T

R

P

Q

R

L

V

M

M

E

E

R

A

L

L

E

R

-

M

-
x
V

-

Q

-

L

Q

E

N

T

I

F

H

A

P

Q

D

R

T

K

P

P

L

-

G

H

N

Q

L

L

R

S

I

G

G

G

Q

Q

I

R

V

V

E

A

I

I

A

A

K

R

A

A

L

V

A

V

Q

N

N

K

A

P

R

R

I

L

L

L

I

L

L

L

D
|
D

E

E

P

-

T

-

S

-

A

S

L

L

S

S

A

A

A

L

E

D

V

Y

E

K

V

L

L

R

F

K

R

Q

V

M

I

Q

D

N

E

E

L

L

T

K

A

A

-

L

-

Q

-

R

-

K

Q

L

G

G

V

I

G

T

I

F

V

V

Y

F

I

V

S

T

H

H

R

D

L

Q

E

E

L

A

I

L

R

T

V

M

G

S

D

D

Y

R

I

I

T

V

V

V

L

M

R

R

D

D

G

G

V

R

I

I

C26 (≠ V17) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y18) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ V36) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A45) mutation to T: Loss of ATPase activity and transport.
L60 (≠ M51) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ M67) mutation to P: Lower ATPase activity and transport efficiency.
V135 (vs. gap) mutation to M: Loss of ATPase activity and transport.
D172 (= D170) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8xfcD Cryo-em structure of the atp-bound mtb dppabcd with the d445a mutation of dppa
28% identity, 92% coverage: 14:482/511 of query aligns to 9:488/517 of 8xfcD

query
sites
8xfcD

G

G

V

L

K

E

V

V

Y

T

P
x
F

G

G

T

T

R

D

A

A

-

P

-

A

L

V

K

C

G

G

V

V

D

D

F

L

D

A

L

V

R

R

M

S

G

G

A

Q

V

T

N

V

V

A

L

V

V

V

G

G

E

E

N

S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

T

M

A

K

A

L

A

I

I

A

L

G

G

V

L

E

L

D

P

-

A

-

G

-

R

M

I

T

T

E

A

G

G

T

R

I

V

T

V

M

F

D

D

G

G

R

R

E

D

M

I

R

-

F

-

R

-

T

T

K

G

A

A

D

D

A

A

V

K

-

R

-

L

-

R

-

S

-

I

-

R

A

G

A

R

G

E

I

I

G

G

I

Y

V

V

F

P

Q

Q

E

D

-

P

-

M

L

T

N

N

L

L

F

N

P

P

N

V

L

W

S

K

V

V

A

G

E

-

N

-

I

-

F

F

I

Q

G

V

H

T

E

E

T

A

T

L

R

R

G

A

G

N

I

T

D

D

I

-

D

-

I

-

E

-

A

-

H

G

R

R

E

A

A

A

T

R

R

R

Q

R

L

A

M

V

E

E

R

L

L

L

E

A

Q

E

N

A

I

G

H

L

P

P

D

D

T

P

P

A

L

K

G

Q

N

A

L

G

R

R

I

Y

G

P

Q

H

Q

Q

-

L

-

S

-

G

-

M

-

C

Q

Q

I

R

V

A

E

L

I

I

A

A

K

I

A

G

L

L

A

A

Q

G

N

R

A

P

R

R

I

L

L

L

I

I

L

A

D

D

E
x
Q

P

P

T

T

S

S

A

A

L

L

S

-

A

-

E

D

V

V

E

T

V

V

L

Q

F

R

R

Q

V

V

I

L

D

D