SitesBLAST
Comparing WP_110806556.1 NCBI__GCF_003217355.1:WP_110806556.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2dtxA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with d-mannose (see paper)
35% identity, 100% coverage: 1:242/243 of query aligns to 2:251/255 of 2dtxA
2dteA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with nadh (see paper)
35% identity, 100% coverage: 1:242/243 of query aligns to 2:251/255 of 2dteA
- active site: G18 (= G17), S132 (= S128), Y145 (= Y141), S148 (= S144), K149 (= K145)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), S16 (≠ R15), M17 (≠ S16), G18 (= G17), I19 (= I18), S38 (≠ T35), I39 (≠ A36), C52 (≠ A49), D53 (= D50), V54 (≠ I51), N80 (= N77), A81 (= A78), I130 (= I126), S132 (= S128), Y145 (= Y141), K149 (= K145), P174 (= P170), A175 (≠ G171), T176 (≠ W172), I177 (= I173), T179 (= T175), P180 (≠ E176), L181 (= L177), V182 (vs. gap)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
37% identity, 98% coverage: 3:239/243 of query aligns to 4:255/255 of 5itvA
- active site: G18 (= G17), S141 (= S128), Y154 (= Y141), K158 (= K145)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), S17 (= S16), G18 (= G17), I19 (= I18), D38 (≠ Q37), I39 (vs. gap), T61 (≠ A49), I63 (= I51), N89 (= N77), G91 (= G79), T139 (≠ I126), S141 (= S128), Y154 (= Y141), K158 (= K145), P184 (= P170), G185 (= G171), I186 (≠ W172), I187 (= I173)
5vt6A Crystal structure of acetoacetyl-coa reductase from burkholderia pseudomallei 1710b complexed with NADP
38% identity, 94% coverage: 7:235/243 of query aligns to 2:241/245 of 5vt6A
- active site: G12 (= G17), D102 (= D93), S138 (= S128), Y151 (= Y141), K155 (= K145)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), G11 (≠ S16), G12 (= G17), L13 (≠ I18), H32 (vs. gap), S33 (vs. gap), N36 (vs. gap), V58 (≠ A49), D59 (= D50), V60 (≠ I51), N86 (= N77), A87 (= A78), G88 (= G79), I89 (≠ V80), I136 (= I126), Y151 (= Y141), K155 (= K145), P181 (= P170), Y183 (≠ W172), L184 (≠ I173), T186 (= T175)
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
36% identity, 97% coverage: 1:235/243 of query aligns to 1:245/249 of 4bmsF
- active site: S137 (= S128), H147 (= H138), Y150 (= Y141), K154 (= K145), Q195 (≠ M185)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (≠ R15), S16 (= S16), I18 (= I18), R38 (= R38), R39 (≠ G39), A59 (= A49), D60 (= D50), V61 (≠ I51), N87 (= N77), S88 (≠ A78), G89 (= G79), V110 (≠ M100), S137 (= S128), Y150 (= Y141), K154 (= K145), G181 (= G171), I183 (= I173), T185 (= T175), I187 (≠ L177)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
35% identity, 96% coverage: 2:235/243 of query aligns to 1:236/239 of 4nbtA
- active site: G16 (= G17), S132 (= S128), Y145 (= Y141), K149 (= K145)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), K15 (≠ S16), G16 (= G17), L17 (≠ I18), D36 (≠ Q37), L37 (≠ R38), L52 (≠ A49), N53 (≠ D50), V54 (≠ I51), N80 (= N77), A81 (= A78), G82 (= G79), I130 (= I126), S132 (= S128), Y145 (= Y141), K149 (= K145), P177 (= P170), G178 (= G171), I180 (= I173), T182 (= T175)
7xqmB Indel-mutant short chain dehydrogenase bound to sah (see paper)
42% identity, 99% coverage: 1:240/243 of query aligns to 1:246/253 of 7xqmB
- binding s-adenosyl-l-homocysteine: G13 (= G13), I16 (= I18), D34 (≠ Q37), L35 (≠ R38), R36 (≠ G39), V53 (≠ A49), L55 (≠ I51), N81 (= N77), A82 (= A78), A83 (≠ G79), Y146 (= Y141)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
37% identity, 95% coverage: 7:238/243 of query aligns to 6:247/248 of 6ixmC
- active site: G16 (= G17), S142 (= S128), Y155 (= Y141), K159 (= K145)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S15 (= S16), G16 (= G17), I17 (= I18), D36 (≠ Q37), I37 (≠ R38), A61 (vs. gap), D62 (= D53), T63 (= T54), N89 (= N77), A90 (= A78), M140 (≠ I126), S142 (= S128), Y155 (= Y141), K159 (= K145), P185 (= P170), A186 (≠ G171), Y187 (≠ W172), I188 (= I173), L192 (= L177)
6ihhA Crystal structure of rasadh f12 from ralstonia.Sp in complex with NADPH and a6o
36% identity, 97% coverage: 1:235/243 of query aligns to 1:245/249 of 6ihhA
- binding (2R,3S)-2-ethyl-2-[(2E)-2-(6-methoxy-3,4-dihydro-2H-naphthalen-1-ylidene)ethyl]-3-oxidanyl-cyclopentan-1-one: S137 (= S128), H147 (= H138), Y150 (= Y141), L188 (≠ N178)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (≠ R15), S16 (= S16), G17 (= G17), I18 (= I18), R38 (= R38), R39 (≠ G39), D60 (= D50), V61 (≠ I51), N87 (= N77), S88 (≠ A78), G89 (= G79), V110 (≠ M100), T135 (≠ I126), S137 (= S128), Y150 (= Y141), K154 (= K145), P180 (= P170), G181 (= G171), A182 (≠ W172), I183 (= I173), T185 (= T175), S187 (≠ L177)
Sites not aligning to the query:
2d1yA Crystal structure of tt0321 from thermus thermophilus hb8 (see paper)
42% identity, 98% coverage: 4:240/243 of query aligns to 3:240/240 of 2d1yA
- active site: G16 (= G17), S135 (= S128), N145 (≠ H138), Y148 (= Y141), K152 (= K145)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), R15 (≠ S16), I17 (= I18), D36 (≠ Q37), L37 (≠ R38), R38 (≠ G39), V55 (≠ A49), D56 (= D50), L57 (≠ I51), N83 (= N77), A84 (= A78), A85 (≠ G79), I86 (≠ V80), V133 (≠ I126), S135 (= S128), Y148 (= Y141), K152 (= K145), P178 (= P170), G179 (= G171), I181 (= I173), T183 (= T175), A185 (= A184), V186 (≠ M185)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 98% coverage: 1:237/243 of query aligns to 2:243/244 of 4nbuB
- active site: G18 (= G17), N111 (= N101), S139 (= S128), Q149 (≠ H138), Y152 (= Y141), K156 (= K145)
- binding acetoacetyl-coenzyme a: D93 (≠ E83), K98 (≠ A88), S139 (= S128), N146 (= N135), V147 (≠ P136), Q149 (≠ H138), Y152 (= Y141), F184 (≠ W172), M189 (≠ F181), K200 (≠ A190)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ S16), G18 (= G17), I19 (= I18), D38 (≠ Q37), F39 (≠ R38), V59 (vs. gap), D60 (= D50), V61 (≠ I51), N87 (= N77), A88 (= A78), G89 (= G79), I90 (≠ V80), T137 (≠ I126), S139 (= S128), Y152 (= Y141), K156 (= K145), P182 (= P170), F184 (≠ W172), T185 (≠ I173), T187 (= T175), M189 (≠ F181)
P14697 Acetoacetyl-CoA reductase; EC 1.1.1.36 from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha) (see 2 papers)
37% identity, 94% coverage: 7:235/243 of query aligns to 4:242/246 of P14697
- GGI 13:15 (≠ SGI 16:18) binding NADP(+)
- G35 (vs. gap) binding NADP(+)
- R40 (vs. gap) binding NADP(+)
- Q47 (≠ A36) mutation to L: 2.4-fold increase in activity. 2-fold decrease in affinity for NADPH and 2.8-fold decrease in affinity for acetoacetyl-CoA.
- GNV 60:62 (≠ ADI 49:51) binding NADP(+)
- NAGIT 88:92 (≠ NAGVM 77:81) binding NADP(+)
- D94 (≠ E83) mutation to A: About 6% of wild-type activity.
- K99 (≠ A88) mutation to A: Nearly loss of activity.
- Q147 (≠ N135) mutation to A: About 30% of wild-type activity.
- F148 (≠ P136) mutation to A: About 30% of wild-type activity.
- Q150 (≠ H138) mutation to A: About 20% of wild-type activity.
- T173 (= T161) mutation to S: 3.5-fold increase in activity. 4-fold decrease in affinity for NADPH and 2.4-fold decrease in affinity for acetoacetyl-CoA.
- PGYI 183:186 (≠ PGWI 170:173) binding NADP(+)
- Y185 (≠ W172) mutation to A: Nearly loss of activity.
- R195 (≠ V182) mutation to A: Nearly loss of activity.
3vzsB Crystal structure of phab from ralstonia eutropha in complex with acetoacetyl-coa and NADP (see paper)
37% identity, 94% coverage: 7:235/243 of query aligns to 7:245/249 of 3vzsB
- active site: N115 (= N101), S143 (= S128), Y156 (= Y141), K160 (= K145)
- binding acetoacetyl-coenzyme a: D97 (≠ E83), Q150 (≠ N135), F151 (≠ P136), Q153 (≠ H138), Y156 (= Y141), G187 (= G171), Y188 (≠ W172), R198 (≠ V182)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), I18 (= I18), G38 (vs. gap), R43 (vs. gap), G63 (≠ A49), N64 (≠ D50), V65 (≠ I51), G93 (= G79), I94 (≠ V80), T95 (≠ M81), P186 (= P170), I189 (= I173), M193 (≠ L177), V194 (≠ N178)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
35% identity, 94% coverage: 7:235/243 of query aligns to 5:243/246 of 3osuA
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
35% identity, 99% coverage: 3:243/243 of query aligns to 4:250/252 of 6vspB
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
35% identity, 99% coverage: 3:243/243 of query aligns to 2:248/251 of 6xewA
- active site: G16 (= G17), S138 (= S128), Y151 (= Y141)
- binding r,3-hydroxybutan-2-one: S138 (= S128), S140 (≠ E130), Y151 (= Y141)
- binding s,3-hydroxybutan-2-one: S138 (= S128), Y151 (= Y141), S182 (≠ G171)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ S16), G16 (= G17), M17 (≠ I18), D36 (≠ Q37), W37 (≠ R38), W37 (≠ R38), A38 (≠ G39), I59 (≠ A49), D60 (= D50), V61 (≠ I51), N87 (= N77), A88 (= A78), G89 (= G79), V110 (≠ M100), T136 (≠ I126), S138 (= S128), Y151 (= Y141), K155 (= K145), S182 (≠ G171), L183 (≠ W172), V184 (≠ I173), T186 (= T175), N187 (≠ E176), M188 (≠ L177), T189 (≠ N178)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
35% identity, 99% coverage: 3:243/243 of query aligns to 2:248/251 of H9XP47