SitesBLAST
Comparing WP_110806731.1 NCBI__GCF_003217355.1:WP_110806731.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P60752 ATP-dependent lipid A-core flippase; Lipid A export ATP-binding/permease protein MsbA; Lipid flippase; EC 7.5.2.6 from Escherichia coli (strain K12) (see 7 papers)
33% identity, 95% coverage: 28:590/592 of query aligns to 10:580/582 of P60752
- C88 (≠ L100) mutation to S: Does not affect ATPase activity.
- E208 (≠ D220) mutation to A: Does not reduce substrate binding or nucleotide binding, but decreases ATP-dependent extrusion of substrates. Inhibits formation of outward-facing conformation.; mutation to C: Exhibits ATPase activity. Forms intermolecular cross-links.; mutation to Q: Improves basal ATPase activity and increases transport activity.
- K212 (≠ H224) mutation to A: Does not reduce substrate binding or nucleotide binding, but decreases ATP-dependent extrusion of substrates.
- A270 (= A282) mutation to T: Temperature-sensitive. Loss of lipid export to the outer membrane. Significantly decreases ATPase activity at 42 degrees Celsius but not at 30 degrees Celsius.
- C315 (≠ L327) mutation to S: Does not affect ATPase activity.
- E506 (= E516) mutation to Q: Lacks cell viability and does not support growth. Can still bind ATP and slowly hydrolyze ATP, but becomes locked into a closed dimer conformation.
- L511 (= L521) mutation to P: Loss of ATPase activity; ATP is still bound.
- D512 (= D522) mutation to G: Loss of ATPase activity; ATP is still bound.
- H537 (= H547) mutation to A: Lacks cell viability and does not support growth. Can still bind ATP and slowly hydrolyze ATP, but becomes locked into a closed dimer conformation.
8dmmA Structure of the vanadate-trapped msba bound to kdl (see paper)
33% identity, 95% coverage: 28:590/592 of query aligns to 6:576/576 of 8dmmA
- binding adp orthovanadate: Y347 (= Y364), R350 (≠ K367), S374 (= S389), G375 (= G390), S376 (≠ A391), G377 (= G392), K378 (= K393), S379 (= S394), T380 (≠ S395), Q420 (= Q435), L476 (≠ A490), S478 (= S492), G479 (= G493), G480 (= G494), H533 (= H547)
- binding (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-5-oxidanyl-oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid: V25 (= V41), Y79 (≠ V95), Y83 (≠ T99), R184 (= R199), R234 (= R250), K239 (= K255), I246 (= I262), I250 (≠ L266)
7mewA E. Coli msba in complex with g247 (see paper)
33% identity, 95% coverage: 28:589/592 of query aligns to 3:572/572 of 7mewA
6bppA E. Coli msba in complex with lps and inhibitor g092 (see paper)
33% identity, 95% coverage: 28:589/592 of query aligns to 7:576/576 of 6bppA
- binding (2E)-3-{6-[(1S)-1-(3-amino-2,6-dichlorophenyl)ethoxy]-4-cyclopropylquinolin-3-yl}prop-2-enoic acid: L168 (≠ A183), A172 (= A187), V175 (≠ L190), S176 (vs. gap), I179 (≠ P193), A256 (≠ T271), M288 (= M303), L291 (≠ T306), M292 (≠ F307), K296 (≠ R311)
6bplA E. Coli msba in complex with lps and inhibitor g907 (see paper)
33% identity, 95% coverage: 28:589/592 of query aligns to 7:576/576 of 6bplA
- binding (2E)-3-{6-[(1S)-1-(2-chloro-6-cyclopropylphenyl)ethoxy]-4-cyclopropylquinolin-3-yl}prop-2-enoic acid: F154 (= F169), L168 (≠ A183), V175 (≠ L190), S176 (vs. gap), I179 (≠ P193), A256 (≠ T271), M288 (= M303), L291 (≠ T306), M292 (≠ F307), L295 (= L310), K296 (≠ R311)
7bcwA Structure of msba in salipro with adp vanadate (see paper)
33% identity, 95% coverage: 28:589/592 of query aligns to 5:574/574 of 7bcwA
- binding adenosine-5'-diphosphate: Y346 (= Y364), G374 (= G390), S375 (≠ A391), K377 (= K393), S378 (= S394), T379 (≠ S395), L475 (≠ A490), S477 (= S492), Q480 (= Q495)
- binding magnesium ion: S378 (= S394), Q419 (= Q435)
- binding vanadate ion: S373 (= S389), K377 (= K393), S477 (= S492), A505 (= A520), H532 (= H547)
7ph3A Amp-pnp bound nanodisc reconstituted msba with nanobodies, spin- labeled at position a60c (see paper)
33% identity, 95% coverage: 28:589/592 of query aligns to 8:577/577 of 7ph3A
- binding phosphoaminophosphonic acid-adenylate ester: Y349 (= Y364), S376 (= S389), G377 (= G390), G379 (= G392), K380 (= K393), S381 (= S394), T382 (≠ S395), Q422 (= Q435), L478 (≠ A490), S480 (= S492), G482 (= G494), Q483 (= Q495), H535 (= H547)
- binding magnesium ion: S381 (= S394), Q422 (= Q435)
7ph2A Nanodisc reconstituted msba in complex with nanobodies, spin-labeled at position a60c (see paper)
33% identity, 94% coverage: 32:590/592 of query aligns to 2:569/569 of 7ph2A