SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_111391826.1 NCBI__GCF_003253485.1:WP_111391826.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
33% identity, 65% coverage: 26:238/326 of query aligns to 24:236/265 of P07821

query
sites
P07821

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K50 (= K52) mutation to Q: Lack of activity.
D172 (= D174) mutation to E: Lack of activity.
E173 (= E175) mutation to A: Lack of activity.

5d3mA Folate ecf transporter: amppnp bound state (see paper)
33% identity, 67% coverage: 19:237/326 of query aligns to 13:230/280 of 5d3mA

query
sites
5d3mA

Y
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binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y19), N42 (= N48), G43 (= G49), K46 (= K52), S47 (= S53)

8bmpA Cryo-em structure of the folate-specific ecf transporter complex in msp2n2 lipid nanodiscs bound to atp and adp (see paper)
34% identity, 64% coverage: 29:237/326 of query aligns to 20:227/278 of 8bmpA

query
sites
8bmpA

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binding adenosine-5'-triphosphate: N39 (= N48), G42 (= G51), K43 (= K52), S44 (= S53), T45 (= T54)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13

O65934 ABC transporter ATP-binding/permease protein Rv1747; EC 7.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
33% identity, 62% coverage: 26:228/326 of query aligns to 332:537/865 of O65934

query
sites
O65934

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E479 (= E175) mutation to Q: Loss of ATPase activity.

Sites not aligning to the query:

33 R→A: Strong decrease in phosphorylation.
47 S→A: Strong decrease in phosphorylation. Lack of interaction with PknF. Attenuates growth in macrophages.
69 N→A: Strong decrease in phosphorylation.
152 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-210.
210 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-152.
234 R→A: Strong decrease in phosphorylation.
248 S→A: Strong decrease in phosphorylation. Decreases interaction with PknF.
270 N→A: Strong decrease in phosphorylation.

8bmsA Cryo-em structure of the mutant solitary ecf module 2eq in msp2n2 lipid nanodiscs in the atpase closed and atp-bound conformation (see paper)
34% identity, 64% coverage: 29:237/326 of query aligns to 20:227/278 of 8bmsA

query
sites
8bmsA

L

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binding adenosine-5'-triphosphate: N39 (= N48), G40 (= G49), S41 (≠ V50), G42 (= G51), K43 (= K52), S44 (= S53), T45 (= T54), Q88 (≠ T94), Y133 (≠ L142), N140 (≠ E149), S142 (= S151), G143 (≠ D152), G144 (= G153), Q145 (= Q154), Q166 (≠ E175), H199 (= H208)
binding magnesium ion: S44 (= S53), Q88 (≠ T94)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

5x40A Structure of a cbio dimer bound with amppcp (see paper)
34% identity, 64% coverage: 29:238/326 of query aligns to 21:228/280 of 5x40A

query
sites
5x40A

L

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V

A

S

L

F

G

G

R

P

T

L

P

-

H

N

T

L

G

G

W

L

S

S

G

-

K

-

L

-

N

E

P

A

A

E

D

A

R

R

D

A

V

R

V

V

E

E

K

E

A

A

L

L

S

A

D

A

T

L

K

S

I

I

T

S

Y

D

L

L

R

R

D

D

E

R

R

P

L

T

S
x
H

E
x
M

I
x
L

S
|
S

D

G

G
|
G

Q
|
Q

R

K

Q

R

K

R

A

V

M

A

I

I

A

A

R

G

A

A

L

V

A

A

Q

M

D

R

G

P

Q

E

V

V

M

L

I

L

L

L

D

D

E
x
Q

P

P

T

T

A

A

H

G

L

L

D

D

L

L

V

A

N

G

R

T

F

E

E

Q

I

L

M

L

S

T

L

L

R

R

D

G

I

L

A

-

H

R

A

A

Q

A

R

G

K

M

A

T

I

L

L

V

V

F

V

S

T

T

H
|
H

D

D

L

V

D

E

I

L

A

A

I

A

E

A

T

L

A

A

D

D

R

R

F

V

W

A

L

L

L

F

N

R

C

T

G

G

T

R

P

V

L

L

I

A

S

E

G

G

K

A

P

A

E

E

D

A

L

V

I

L

binding phosphomethylphosphonic acid adenylate ester: N40 (= N48), G41 (= G49), G43 (= G51), K44 (= K52), S45 (= S53), T46 (= T54), Q88 (≠ T94), H139 (≠ S148), M140 (≠ E149), L141 (≠ I150), S142 (= S151), G144 (= G153), Q145 (= Q154), Q166 (≠ E175), H198 (= H208)
binding magnesium ion: S45 (= S53), Q88 (≠ T94)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 14, 18, 20

8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
31% identity, 64% coverage: 19:226/326 of query aligns to 14:214/257 of 8wm7D

query
sites
8wm7D

Y
|
Y

F

P

H

T

Q

P

K

E

A

G

K

P

K
x
Y

E

T

I
x
V

L

L

S

D

N

G

L

I

D

D

F

L

T

K

L

V

F

R

S

E

G

G

E

E

L

F

T

V

C

C

L

L

L

I

G

G

P

H

N
x
S

G
|
G

V
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

N

A

M

I

I

L

S

G

G

Q

F

I

N

H

T

P

P

C

S

K

E

G

G

S

V

I

V

Q

L

L

L

D

Q

Q

D

R

K

S

P

I

I

T

T

D

-

Y

-

S

-

I

-

E

E

E

P

L

G

A

P

K

D

R

R

I

M

A

M

V

V

V

F

L

Q

T

N

D

Y

P

C

V

L

F

L

P

P

G

W

N

-

M

L

T

N

V

V

G

F

Q

E

L

N

V

V

A

Y

L

L

G

A

R

V

T

-

P

-

H

D

T

A

G

V

W

F

S

P

G

N

K

K

L

P

N

Q

P

A

A

E

D

K

R

R

D

A

V

I

V

V

E

R

K

E

A

H

L

L

S

A

D

M

T

V

K

G

I

L

T

T

Y

E

L

A

R

A

D

E

E

K

R

K

L

P

S

S

E

Q

I

I

S

S

D

G

G

G

Q

M

R

K

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

S

Q

I

D

R

G

P

Q

Q

V

V

M

L

I

I

L

L

D

D

E

E

P

P

T

F

A

G

H

A

L

L

D

D

L

A

V

I

N

T

R

K

F

E

E

E

I

L

M

Q

S

E

L

E

L

L

R

L

D

Q

I

I

A

W

H

S

A

D

Q

H

R

Q

K

V

A

T

I

V

L

L

V

M

V

I

T

T

H

H

D

D

L

I

D

D

I

E

A

A

I

L

E

F

T

L

A

A

D

D

R

R

F

V

W

V

L

M

N

T

C

N

G

G

binding adenosine-5'-diphosphate: Y14 (= Y19), Y21 (≠ K26), V23 (≠ I28), S43 (≠ N48), G44 (= G49), C45 (≠ V50), G46 (= G51), K47 (= K52), S48 (= S53), T49 (= T54)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
31% identity, 64% coverage: 28:237/326 of query aligns to 17:223/241 of 4u00A

query
sites
4u00A

I
x
V

L

L

S

K

N

G

L

I

D

H

F

L

T

E

L

V

F

A

S

P

G

G

E

E

L

K

T

L

C

V

L

I

G

G

P

P

N
x
S

G
|
G

V
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

A

T

I

I

L

N

G

R

Q

L

I

E

H

D

P

F

C

Q

K

E

G

G

S

E

I

V

Q

V

L

V

D

D

Q

G

R

L

S

S

I

V

T

K

D

D

-

D

Y

R

S

A

I

L

E

R

E

E

L

I

A

R

K

R

R

E

I

V

A

G

V

M

V

V

L

F

T

Q

D

Q

-

F

P

N

V

L

F

F

P

P

G

-

N

H

M

M

T

T

V

V

G

L

Q

E

L

N

V

V

A

T

L

L

G

A

R

P

T

M

P

R

H

V

T

R

G

R

W

W

S

P

G

R

K

E

L

-

N

-

P

K

A

A

D

E

R

K

D

K

V

A

V

L

E

E

K

-

A

L

L

L

S

E

D

R

T

V

K

G

I

I

T

L

Y

D

L

Q

R

A

D

R

E

K

R

Y

L

P

S

A

E

Q

I

L

S

S

D

G

G

G

Q

Q

R

Q

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

M

D

E

G

P

Q

K

V

I

M

M

I

L

L

F

D

D

E

E

P

P

T

T

A

S

H

A

L

L

D

D

L

P

V

E

N

M

R

V

F

G

E

E

I

V

M

L

S

D

L

V

L

M

R

R

D

D

I

L

A

A

H

Q

A

G

Q

G

R

-

K

M

A

T

I

M

L

V

V

V

T

T

H

H

D

E

L

M

D

G

I

F

A

A

I

R

E

E

T

V

A

A

D

D

R

R

F

V

W

V

L

F

L

M

N

D

C

G

G

G

T

Q

P

I

L

V

I

E

S

E

G

G

K

R

P

P

E

E

D

E

L

I

binding adenosine-5'-diphosphate: V17 (≠ I28), S37 (≠ N48), G38 (= G49), S39 (≠ V50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

5eg1B Antibacterial peptide abc transporter mcjd with a resolved lipid (see paper)
30% identity, 74% coverage: 3:242/326 of query aligns to 335:567/576 of 5eg1B

query
sites
5eg1B

R

N

I

M

Q

E

K

R

H

K

I

L

A

N

L

L

S

S

G

I

K

R

E

E

L

L

A

S

L

F

G

S

Y
|
Y

F

-

H

-

Q

-

K

S

A

D

K

D

K

K

E

K

I

I

L

L

S

N

N

S

L

V

D

S

F

L

T

D

L

L

F

F

S

T

G

G

E

K

L

M

T

Y

C

S

L

L

L

T

G

G

P

P

N
x
S

G
|
G

V
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

V

K

K

A

I

I

I

L

S

G

G

Q

Y

I

Y

H

K

P

N

C

Y

K

F

G

G

S

D

I

I

Q

Y

L

L

D

N

Q

D

R

I

S

S

I

L

T

R

D

N

Y

I

S

S

I

D

E

E

E

D

L

L

A

N

K

D

R

A

I

I

A

Y

V

Y

V

L

L

T

T
x
Q

D

D

P

D

-

Y

V

I

F

F

P

M

G

D

N

T

M

L

T

R

V

F

G

N

Q

L

L

R

V

L

A

A

L

N

G

Y

R

D

T

A

P

S

H

E

T

N

G

E

W

I

S

F

G

K

K

V

L

L

N

K

P

L

A

A

D

N

R

L

D

S

V

V

-

N

E

E

K

P

A

V

L

S

S

L

D

D

T

T

K

H

I

L

T

I

Y

-

L

-

R

-

D

-

E

N

R

R

L

G

S
x
N

E
x
N

I

Y

S
|
S

D
x
G

G
|
G

Q
|
Q

R

K

Q

Q

K

R

A

I

M

S

I

L

A

A

R

R

A

L

L

F

A

L

Q

R

D

K

G

P

Q

A

V

I

M

I

I

I

L

I

D

D

E

E

P

A

T

T

A

S

H

A

L

L

D

D

L

Y

V

I

N

N

R

E

F

S

E

E

I

I

M

L

S

S

L

S

L

I

R

R

D

-

I

-

A

T

H

H

A

F

Q

P

R

D

K

A

A

L

I

I

L

I

V

N

V

I

T

S

H

H

D

R

L

I

D

N

I

L

A

-

I

L

E

E

T

C

A

S

D

D

R

C

F

V

W

Y

L

V

L

L

N

N

C

E

G

G

T

N

P

I

L

V

I

A

S

S

G

G

K

H

P

F

E

R

D

D

L

L

I

M

L

V

S

S

G

N

Q

E

binding phosphoaminophosphonic acid-adenylate ester: Y351 (= Y19), S377 (≠ N48), G378 (= G49), S379 (≠ V50), G380 (= G51), K381 (= K52), S382 (= S53), T383 (= T54), Q423 (≠ T94), N476 (≠ S148), N477 (≠ E149), S479 (= S151), G480 (≠ D152), G481 (= G153), Q482 (= Q154)
binding magnesium ion: S382 (= S53), Q423 (≠ T94)

Sites not aligning to the query:

binding (2s)-3-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-2-[(6e)-hexadec-6-enoyloxy]propyl (8e)-octadec-8-enoate: 152, 155, 183

5ofrA Structure of the antibacterial peptide abc transporter mcjd in a high energy outward occluded intermediate state (see paper)
30% identity, 74% coverage: 3:242/326 of query aligns to 330:562/571 of 5ofrA

query
sites
5ofrA

R

N

I

M

Q

E

K

R

H

K

I

L

A

N

L

L

S

S

G

I

K

R

E

E

L

L

A

S

L

F

G

S

Y
|
Y

F

-

H

-

Q

-

K

S

A

D

K

D

K

K

E

K

I
|
I

L

L

S

N

N

S

L

V

D

S

F

L

T

D

L

L

F

F

S

T

G

G

E

K

L

M

T

Y

C

S

L

L

L

T

G

G

P

P

N
x
S

G
|
G

V
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

V

K

K

A

I

I

I

L

S

G

G

Q

Y

I

Y

H

K

P

N

C

Y

K

F

G

G

S

D

I

I

Q

Y

L

L

D

N

Q

D

R

I

S

S

I

L

T

R

D

N

Y

I

S

S

I

D

E

E

E

D

L

L

A

N

K

D

R

A

I

I

A

Y

V

Y

V

L

L

T

T
x
Q

D

D

P

D

-

Y

V

I

F

F

P

M

G

D

N

T

M

L

T

R

V

F

G

N

Q

L

L

R

V

L

A

A

L

N

G

Y

R

D

T

A

P

S

H

E

T

N

G

E

W

I

S

F

G

K

K

V

L

L

N

K

P

L

A

A

D

N

R

L

D

S

V

V

-
x
N

-

N

E

E

K

P

A

V

L

S

S

L

D

D

T

T

K

H

I

L

T

I

Y

-

L

-

R

-

D

-

E

N

R

R

L

G

S
x
N

E
x
N

I

Y

S
|
S

D

G

G

G

Q
|
Q

R

K

Q

Q

K

R

A

I

M

S

I

L

A

A

R

R

A

L

L

F

A

L

Q

R

D

K

G

P

Q

A

V

I

M

I

I

I

L

I

D

D

E

E

P

A

T

T

A

S

H

A

L

L

D

D

L

Y

V

I

N

N

R

E

F

S

E

E

I

I

M

L

S

S

L

S

L

I

R

R

D

-

I

-

A

T

H

H

A

F

Q

P

R

D

K

A

A

L

I

I

L

I

V

N

V

I

T

S

H

H

D

R

L

I

D

N

I

L

A

-

I

L

E

E

T

C

A

S

D

D

R

C

F

V

W

Y

L

V

L

L

N

N

C

E

G

G

T

N

P

I

L

V

I

A

S

S

G

G

K

H

P

F

E

R

D

D

L

L

I

M

L

V

S

S

G

N

Q

E

binding adenosine-5'-diphosphate: Y346 (= Y19), I352 (= I28), S372 (≠ N48), G373 (= G49), S374 (≠ V50), G375 (= G51), K376 (= K52), S377 (= S53), T378 (= T54), N456 (vs. gap), N471 (≠ S148), N472 (≠ E149), S474 (= S151), Q477 (= Q154)
binding magnesium ion: S377 (= S53), Q418 (≠ T94)

Q00748 Multidrug resistance protein homolog 65; P-glycoprotein 65; EC 7.6.2.2 from Drosophila melanogaster (Fruit fly) (see paper)
30% identity, 89% coverage: 19:308/326 of query aligns to 411:716/1302 of Q00748

query
sites
Q00748

Y

F

F

F

H

R

Q

Y

K

P

A

S

K

R

K

P

E

E

I

V

L

I

-

V

-

H

S

R

N

G

L

L

D

N

F

I

T

R

L

I

F

R

S

A

G

G

E

Q

L

T

T

V

C

A

L

L

L

V

G

G

P

S

N

S

G

G

V

C

G

G

K

K

S

S

T

T

L

C

I

V

K

Q

A

L

I

L

L

Q

G

R

Q

F

I

Y

H

D

P

P

C

V

K

F

G

G

S

S

I

V

Q

L

L

L

D

D

Q

D

R

L

S

D

I

I

T

R

D

K

Y

Y

S

N

I

I

E

Q

E

W

L

L

A

R

K

S

R

N

I

I

A

A

V

V

L

G

T

Q

D

E

P

P

V

V

-

L

F

F

P

L

G

G

N

-

M

-

T

T

V

I

G

A

Q

Q

L

N

V

I

A

S

L

Y

G

G

R

K

T

-

P

P

H

G

T

A

G

T

W

Q

S

K

G

E

K

I

L

E

N

A

P

A

A

A

D

T

R

Q

D

A

V

G

V

A

E

H

K

E

A

F

L

I

S

T

D

N

T

L

K

P

I

E

T

S

Y

Y

-

R

-

S

L

M

R

I

D

G

E

E

R

R

L

G

S

S

E

Q

I

L

S

S

D

G

G

G

Q

Q

R

K

Q

Q

K

R

A

I

M

A

I

I

A

A

R

R

A

A

L

L

A

I

Q

Q

D

N

G

P

Q

K

V

I

M

L

I

L

L

L

D

D

E

E

P

A

T

T

A

S

H

A

L

L

D

D

L

Y

V

Q

N

S

R

E

F

K

E

Q

I

V

M

Q

S

Q

L

A

L

L

R

-

D

D

I

L

A

A

H

S

A

K

Q

G

R

R

K

T

A

T

I

I

L

-

V

V

T

S

H

H

D

R

L

L

D

S

I

-

A

A

I

I

E

R

T

G

A

A

D

D

R

K

F

I

W

V

L

F

L

I

N

H

C

D

G

G

T

K

P

V

L

L

I

E

S

E

G

G

K

S

P

H

E

D

D

D

L

L

-

M

-

A

-

L

-

E

-

G

-

A

-

Y

-

N

-

M

I

V

L

R

S

A

G

G

Q

D

I

I

N

N

Q

-

L

-

L

M

P

P

N

D

-

E

-

V

-

E

-

K

-

E

-

D

-

S

-

I

-

E

N

D

T

T

Y

K

R

Q

F

K

S

S

L

L

S

A

R

L

G

F

R

E

M

K

E

S

A

F

E

E

E

T

P

S

D

P

L

L

N

N

F

F

K

-

I

-

E

E

G

K

P

G

Q

Q

E

K

L

N

I

S

F

V

W

Q

V

F

E

E

K

E

A

P

L

I

I

I

K

K

A

A

G

L

I

I

K

K

-

D

-

T

N

N

L

A

D

Q

S

S

T

A

I

E

S

A

V

P

S

P

H

E

K

K

P

P

F

N

L

F

I

F

R

R

Sites not aligning to the query:

103 modified: carbohydrate, N-linked (GlcNAc...) asparagine

8w9mD Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
31% identity, 64% coverage: 19:226/326 of query aligns to 12:212/256 of 8w9mD

query
sites
8w9mD

Y
|
Y

F

P

H

T

Q

P

K

E

A

G

K

P

K

Y

E

T

I

V

L

L

S

D

N

G

L

I

D

D

F

L

T

K

L

V

F

R

S

E

G

G

E

E

L

F

T

V

C

C

L

L

L

I

G

G

P
x
H

N
x
S

G
|
G

V

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

N

A

M

I

I

L

S

G

G

Q

F

I

N

H

T

P

P

C

S

K

E

G

G

S

V

I

V

Q

L

L

L

D

Q

Q

D

R

K

S

P

I

I

T

T

D

-

Y

-

S

-

I

-

E

E

E

P

L

G

A

P

K

D

R

R

I

M

A

M

V

V

V

F

L
x
Q

T

N

D

Y

P

C

V

L

F

L

P

P

G

W

N

-

M

L

T

N

V

V

G

F

Q

E

L

N

V

V

A

Y

L

L

G

A

R

V

T

-

P

-

H

D

T

A

G

V

W

F

S

P

G

N

K

K

L

P

N

Q

P

A

A

E

D

K

R

R

D

A

V

I

V

V

E

R

K

E

A

H

L

L

S

A

D

M

T

V

K

G

I

L

T

T

Y

E

L

A

R

A

D

E

E

K

R

K

L

P

S

S

E
x
Q

I

I

S
|
S

D

G

G
|
G

Q
x
M

R

K

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

S

Q

I

D

R

G

P

Q

Q

V

V

M

L

I

I

L

L

D

D

E

Q

P

P

T

F

A

G

H

A

L

L

D

D

L

A

V

I

N

T

R

K

F

E

E

E

I

L

M

Q

S

E

L

E

L

L

R

L

D

Q

I

I

A

W

H

S

A

D

Q

H

R

Q

K

V

A

T

I

V

L

L

V

M

V

I

T

T

H
|
H

D

D

L

I

D

D

I

E

A

A

I

L

E

F

T

L

A

A

D

D

R

R

F

V

W

V

L

M

N

T

C

N

G

G

binding adenosine-5'-triphosphate: Y12 (= Y19), H40 (≠ P47), S41 (≠ N48), G42 (= G49), G44 (= G51), K45 (= K52), S46 (= S53), T47 (= T54), Q82 (≠ L93), Q135 (≠ E149), S137 (= S151), G139 (= G153), M140 (≠ Q154), H194 (= H208)
binding magnesium ion: S46 (= S53), Q82 (≠ L93)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
33% identity, 64% coverage: 27:235/326 of query aligns to 22:227/650 of 5ws4A

query
sites
5ws4A

E

Q

I

I

L

L

S

K

N

G

L

I

D

D

F

L

T

T

L

I

F

Y

S

E

G

G

E

E

L

L

T

V

C

A

L

I

L

V

G

G

P

Q

N
x
S

G
|
G

V

S

G

G

K

K

S
|
S

T

T

L

L

I

M

K

N

A

-

I

I

L

L

G

G

Q

C

I

L

-

D

H

R

P

P

C

T

K

S

G

G

S

S

I

Y

Q

K

L

V

D

N

Q

G

R

Q

S

E

I

T

T

G

D

K

Y

L

S

E

I

P

E

D

E

Q

L

L

A

A

K

Q

R

-

I

L

A

R

V

R

V

E

L

Y

T

F

D

G

P

F

V

I

F

F

P

Q

G

R

N

Y

M

H

T

L

V

L

G

G

Q

D

L

L

V

S

A

A

L

E

G

G

R

N

T

V

P

E

H

V

T

P

G

A

W

V

S

Y

G

A

K

G

L

V

N

T

P

P

A

A

D

D

R

R

D

K

V

Q

V

R

E

A

K

T

A

A

L

L

-

L

S

T

D

E

T

L

K

G

I

L

T

G

Y

T

L

K

R

T

D

Q

E

N

R

R

L

P

S

S

E

Q

I

L

S

S

D

G

G

G

Q

Q

R

Q

Q

Q

K

R

A

V

M

S

I

I

A

A

R

R

A

A

L

L

A

M

Q

N

D

G

G

G

Q

D

V

V

M

I

I

L

L

A

D

D

E

E

P

P

T

T

A

G

H

A

L

L

D

D

L

S

V

H

N

S

R

G

F

V

E

E

I

V

M

M

S

R

L

I

L

L

R

R

D

E

I

L

A

-

H

N

A

A

Q

A

R

G

K

H

A

T

I

I

L

I

V

L

V

V

T

T

H

H

D

D

L

M

D

Q

I

V

A

A

I

-

E

K

T

N

A

A

D

T

R

R

F

I

W

I

L

E

L

I

N

S

C

D

G

G

T

-

P

E

L

I

I

I

S

S

G

D

K

R

P

P

E

N

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (≠ N48), G44 (= G49), S48 (= S53)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 78% coverage: 26:278/326 of query aligns to 30:290/378 of P69874

query
sites
P69874

K

K

E

E

I

V

L

I

S

P

N

Q

L

L

D

D

F

L

T

T

L

I

F

N

S

N

G

G

E

E

L
x
F

T

L

C

T

L

L

G

G

P

P

N

S

G

G

V
x
C

G

G

K

K

S

T

T

T

L

V

I
x
L

K

R

A

L

I

I

L

A

G

G

Q

L

I

E

H

T

P

V

C

D

K

S

G

G

S

R

I

I

Q

M

L
|
L

D

D

Q

N

R

E

S

D

I

I

T

T

D

H

Y

V

S

P

I

A

E

E

E

N

L

-

A

-

K

R

R

Y

I

V

A

N

V

T

V

V

L

F

T

Q

D

S

P

Y

V

A

F

L

P

F

G

P

N

H

M

M

T

T

V

V

G

F

Q

E

L

N

V

V

A

A

L

F

G

G

R

L

T

R

P

M

H

Q

T

K

G

T

W

P

S

A

G

A

K

E

L

I

N

T

P

P

A

R

D

-

R

-

D

-

V

-

V

V

E

M

K

E

A

A

L

L

S

R

D

M

T
x
V

K

Q

I

L

T

E

Y

T

L

F

R

A

D

Q

E

R

R

K

L

P

S

H

E

Q

I

L

S

S

D

G

G

G

Q

Q

R

Q

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

V

A

V

Q

N

D

K

G

P

Q

R

V

L

M

L

I

L

L

L

D
|
D

E

E

P

S

T

L

A

S

H

A

L

L

D

D

L

Y

V

K

N

L

R

R

F

K

E

Q

I

M

M

Q

S

N

L

E

L

L

R

K

D

A

I

L

A

Q

H

R

A

K

Q

L

R

G

K

I

A

T

I

F

L

V

V

F

V

V

T

T

H

H

D

D

L

Q

D

E

I

E

A

A

I

L

E

T

T

M

A

S

D

D

R

R

F

I

W

V

L

V

L

M

-

R

-

D

-

G

-

R

-

I

-

E

N

Q

C

D

G

G

T

T

P

P

L

R

-

E

I

I

S

Y

G

E

K

E

P

P

E

K

D

N

L

L

I

F

L

V

S

A

-

G

-

F

-

I

G

G

Q

E

I

I

N

N

Q

M

L

F

L

N

P

A

N

T

N

V

T

I

Y

E

R

R

F

L

S

D

L

E

S

Q

R

R

G

V

R

R

M

A

E

N

A

V

E

E

E

G

P

R

D

E

-
x
C

-

N

-

I

-

Y

L

V

N

N

F

F

K

A

I

V

E

E

G

P

P

G

Q

Q

E

K

L

L

F45 (≠ L41) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ V50) mutation to T: Loss of ATPase activity and transport.
L60 (≠ I56) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L72) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ T137) mutation to M: Loss of ATPase activity and transport.
D172 (= D174) mutation to N: Loss of ATPase activity and transport.
C276 (vs. gap) mutation to A: Lower ATPase activity and transport efficiency.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

P9WQK5 Uncharacterized ABC transporter ATP-binding protein Rv0073 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
30% identity, 64% coverage: 10:219/326 of query aligns to 4:211/330 of P9WQK5

query
sites
P9WQK5

L

L

S

S

G

I

K

Q

E

N

L

L

A

V

L

V

G

E

Y

Y

F

Y

H

S

Q

G

K

G

A

Y

K

A

K

L

E

R

I

P

L

I

S

N

N

G

L

L

D

N

F

L

T

D

L

V

F

A

S

A

G

G

E

S

L

L

T

V

C

M

L

L

G

G

P

P

N

S

G

G

V

C

G

G

K

K

S

T

T

T

L

L

I

L

K

S

A

C

I

L

L

G

G

G

Q

I

I

L

H

R

P

P

C

K

K

S

G

G

S

A

I

I

Q
x
K

L

F

D

D

Q

E

R

V

S

D

I

I

T

T

D

T

Y

L

S

Q

I

G

E

A

E

E

L

L

A

A

K

N

-

Y

-

R

-

N

R

K

I

V

A

G

V

I

V

V

L

F

T

Q

D

A

-

F

P

N

V

L

F

V

P

P

G

S

N

L

M

T

T

A

V

V

G

E

Q

N

L

V

V

M

A

V

L

P

G

L

R

R

T

S

P

-

H

-

T

-

G

-

W

-

S

A

G

G

K

M

L

S

N

R

P

R

A

A

D

S

R

R

D

R

V

R

V

A

E

E

K

E

A

L

L

L

S

A

D

R

T

V

K

N

I

L

T

A

Y

E

L

R

R

M

D

N

E

H

R

R

L

P

S

G

E

D

I

L

S

S

D

G

G

G

Q

Q

R

Q

Q

Q

K

R

A

V

M

A

I

V

A

A

R

R

A

A

L

I

A

A

Q

L

D

D

G

P

Q

P

V

L

M

I

I

L

L

A

D

D

E

E

P

P

T

T

A

A

H

H

L

L

D

D

L

F

V

I

N

Q

R

V

F

E

E

E

I

V

M

L

S

R

L

L

L

I

R

R

D

E

I

L

A

A

H

D

A

G

Q

E

R

R

K

-

A

V

I

V

L

V

V

V

V

A

T

T

H

H

D

D

L

S

D

R

I

M

A

-

I

L

E

P

T

M

A

A

D

D

R

R

K65 (≠ Q71) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8t1pD Heterodimeric abc transporter bmrcd in the occluded conformation bound to adpvi: bmrcd_oc-adpvi (see paper)
32% identity, 72% coverage: 12:246/326 of query aligns to 421:660/665 of 8t1pD

query
sites
8t1pD

G

A

K

K

E

E

L

R

A

A

L

L

G

G

-

R

-

V

-

E

-

F

-

R

-

D

-

V

Y

S

F

F

H

A

Q
x
Y

K

Q

A

E

K

G

K

E

E

E

I
x
V

L

L

S

K

N

H

L

I

D

S

F

F

T

T

L

A

F

Q

S

K

G

G

E

E

L

T

T

V

C

A

L

L

L

V

G

G

P

H

N
x
T

G
|
G

V

S

G
|
G

K
|
K

S
|
S

T
x
S

L

I

I

L

K

N

A

L

I

L

L

F

G

R

Q

F

I

Y

H

D

P

A

C

Q

K

K

G

G

S

D

I

V

Q

L

L

I

D

D

Q

G

R

K

S

S

I

I

T

Y

D

N

Y

M

S

S

I

R

E

Q

E

E

L

L

A

R

K

S

R

H

I

M

A

G

V

I

V

V

L

L

T
x
Q

D

D

P

P

-

Y

V

L

F

F

P

S

G

G

N

-

M

-

T

T

V

I

G

G

Q

S

L

N

V

V

A

S

L

L

G

D

R

D

T

E

P

R

H

M

T

T

G

-

W

-

S

E

G

E

K

E

L

I

N

K

P

N

A

A

D

L

R

R

D

Q

V

V

-

G

-

A

-

E

-

P

-

L

-

K

V

L

E

P

K

K

A

G

L

I

S

N

D

E

T

P

K

V

I

I

T

-

Y

-

L

-

R

-

D

-

E

E

R

K

L

G

S

S

E
x
T

I

L

S
|
S

D

S

G
|
G

Q
x
E

R

R

Q

Q

K

L

A

I

M

S

I

F

A

A

R

R

A

A

L

L

A

A

Q

F

D

D

G

P

Q

A

V

I

M

L

I

I

L

L

D

D

E
|
E

P

A

T

T

A

A

H

H

L

I

D

D

L

T

V

E

N

T

R

E

F

A

E

V

I

I

M

Q

S

K

L

A

L

L

R

-

D

D

I

V

A

V

H

K

A

-

Q

Q

R

G

K

R

A

T

I

T

L

F

V

V

V

I

T

A

H
|
H

D

R

L

L

D

S

I

-

A

T

I

I

E

R

T

N

A

A

D

D

R

Q

F

I

W

L

L

V

L

L

N

D

C

K

G

G

T

E

P

I

L

V

I

E

S

R

G

G

K

N

P

H

E

E

D

E

L

L

I

M

-

A

L

L

S

E

G

G

Q

Q

I

Y

N

Y

Q

Q

L

M

binding adp orthovanadate: Y438 (≠ Q22), V444 (≠ I28), T464 (≠ N48), G465 (= G49), G467 (= G51), K468 (= K52), S470 (≠ T54), E591 (= E175), H622 (= H208)
binding adenosine-5'-triphosphate: T565 (≠ E149), S567 (= S151), G569 (= G153), E570 (≠ Q154)
binding magnesium ion: S469 (= S53), Q510 (≠ T94)

1jj7A Crystal structure of thE C-terminal atpase domain of human tap1 (see paper)
29% identity, 61% coverage: 28:227/326 of query aligns to 29:228/251 of 1jj7A

query
sites
1jj7A

I
x
V

L

L

S

Q

N

G

L

L

D

T

F

F

T

T

L

L

F

R

S

P

G

G

E

E

L

V

T

T

C

A

L

L

L

V

G

G

P

P

N
|
N

G
|
G

V
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

I

A

K

A

A

L

I

L

L

Q

G

N

Q

L

I

Y

H

Q

P

P

C

T

K

G

G

G

S

Q

I

L

Q

L

L

L

D

D

Q

G

R

K

S

P

I

L

T

P

D

Q

Y

Y

S

E

I

H

E

R

E

Y

L

L

A

H

K

R

R

Q

I

V

A

A

V

A

V

V

L

G

T

Q

D

E

P

P

V

Q

F

V

P

F

G

G

N

R

M

-

T

S

V

L

G

Q

Q

E

L

N

V

I

A

A

L

Y

G

G

R

L

T

T

P

Q

H

-

T

-

G

-

W

-

S

-

G

-

K

-

L

-

N

K

P

P

A

T

D

M

R

E

D

E

V

I

V

T

E

A

K

A

A

A

L

V

S

K

D

S

T

G

K

A

I

H

T

S

Y

F

L

I

R

S

-

G

-

L

-

P

-

Q

-

G

-

Y

-

D

-

T

-

E

-

V

D

D

E

E

R

A

L

G

S

S

E

Q

I

L

S

S

D

G

G

G

Q

Q

R

R

Q

Q

K

A

A

V

M

A

I

L

A

A

R

R

A

A

L

L

A

I

Q

R

D

K

G

P

Q

C

V

V

M

L

I

I

L

L

D

D

E

D

P

A

T

T

A

S

H

A

L

L

D

D

L

A

V

N

N

S

R

Q

F

L

E

Q

I

V

M

E

S

Q

L

L

R

Y

D

E

I

S

A

P

H

E

A

R

Q

Y

R

S

K

R

A

S

I

V

L

L

V

L

V

I

T

T

H

Q

D

H

L

L

D

S

I

L

A

-

I

V

E

E

T

Q

A

A

D

D

R

H

F

I

W

L

L

F

L

L

N

E

C

G

G

G

T

A

binding adenosine-5'-diphosphate: V29 (≠ I28), N49 (= N48), G50 (= G49), S51 (≠ V50), G52 (= G51), K53 (= K52), S54 (= S53), T55 (= T54)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 21

6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
27% identity, 66% coverage: 23:237/326 of query aligns to 12:226/253 of 6z5uK

query
sites
6z5uK

K
x
R

A

G

K

E

K

R

E

V

I

I

L

Y

S

D

N

N

L

I

D

S

F

L

T

N

L

I

F

R

S

R

G

G

E

Q

L

I

T

T

C

A

L

I

L

M

G

G

P

P

N
x
S

G
|
G

V

T

G
|
G

K
|
K

S
x
T

T
|
T

L

L

I

L

K

R

A

L

I

I

L

G

G

G

Q

Q

I

L

H

V

P

P

C

D

K

Q

G

G

S

E

I

V

Q

L

L

L

D

D

Q

G

R

K

S

D

I

I

T

A

D

Q

Y

M

S

S

I

R

E

Q

E

E

L

L

-

F

-

A

A

R

K

A

R

R

I

M

A

G

V

M

V

L

L

F

T
x
Q

D

S

P

G

V

A

F

L

P

F

G

T

N

D

M

M

T

S

V

V

G

Y

Q

E

L

N

V

V

A

A

L

F

G

P

R

I

T

R

P

A

H

H

T

T

G

K

W

L

S

S

G

E

K

N

L

L

N

I

P

A

A

-

D

-

R

-

D

E

V

L

V

V

E

A

K

L

A

K

L

L

S

E

D

S

T

V

K

G

I

L

T

R

Y

G

L

T

R

E

D

Q

E

L

R

M

L

P

S

T

E

E

I

L

S

S

D

G

G

G

Q

M

R

N

Q

R

K

R

A

V

M

A

I

L

A

A

R

R

A

A

L

I

A

A

Q

L

D

D

G

P

Q

D

V

L

M

I

I

M

L

Y

D

D

E
|
E

P

P

T

F

A

A

H

G

L

Q

D

D

L

P

V

I

N

V

R

K

F

G

E

V

I

L

M

T

S

R

L

L

L

I

R

R

D

S

I

L

A

R

H

E

A

A

Q

L

R

D

K

L

A

T

I

T

L

I

V

I

V

V

T

S

H
|
H

D

D

L

V

D

P

I

E

A

T

I

L

E

S

T

I

A

A

D

D

R

Y

F

I

W

Y

L

V

N

A

C

E

G

G

T

K

P

I

L

Q

I

G

S

E

G

G

K

T

P

P

E

E

D

E

L

L

binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ K23), S37 (≠ N48), G38 (= G49), G40 (= G51), K41 (= K52), T42 (≠ S53), T43 (= T54), Q86 (≠ T94), E164 (= E175), H197 (= H208)
binding magnesium ion: Q86 (≠ T94), E164 (= E175)

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
27% identity, 66% coverage: 23:237/326 of query aligns to 14:228/263 of 7d0aB

query
sites
7d0aB

K

R

A

G

K

E

K

R

E

V

I

I

L

Y

S

D

N

N

L

I

D

S

F

L

T

N

L

I

F

R

S

R

G

G

E

Q

L

I

T

T

C

A

L

I

L

M

G

G

P

P

N
x
S

G

G

V
x
T

G
|
G

K
|
K

S

T

T

T

L

L

I

L

K

R

A

L

I

I

L

G

G

G

Q

Q

I

L

H

V

P

P

C

D

K

Q

G

G

S

E

I

V

Q

L

L

L

D

D

Q

G

R

K

S

D

I

I

T

A

D

Q

Y

M

S

S

I

R

E

Q

E

E

L

L

-

F

-

A

A

R

K

A

R

R

I

M

A

G

V

M

V

L

L

F

T

Q

D

S

P

G

V

A

F

L

P

F

G

T

N

D

M

M

T

S

V

V

G

Y

Q

E

L

N

V

V

A

A

L

F

G

P

R

I

T

R

P

A

H

H

T

T

G

K

W

L

S

S

G

E

K

N

L

L

N

I

P

A

A

-

D

-

R

-

D

E

V

L

V

V

E

A

K

L

A

K

L

L

S

E

D

S

T

V

K

G

I

L

T

R

Y

G

L

T

R

E

D

Q

E

L

R

M

L

P

S

T

E
|
E

I

L

S
|
S

D

G

G

G

Q
x
M

R

N

Q

R

K

R

A

V

M

A

I

L

A

A

R

R

A

A

L

I

A

A

Q

L

D

D

G

P

Q

D

V

L

M

I

I

M

L

Y

D

D

E

E

P

P

T

F

A

A

H

G

L

Q

D

D

L

P

V

I

N

V

R

K

F

G

E

V

I

L

M

T

S

R

L

L

L

I

R

R

D

S

I

L

A

R

H

E

A

A

Q

L

R

D

K

L

A

T

I

T

L

I

V

I

V

V

T

S

H

H

D

D

L

V

D

P

I

E

A

T

I

L

E

S

T

I

A

A

D

D

R

Y

F

I

W

Y

L

V

N

A

C

E

G

G

T

K

P

I

L

Q

I

G

S

E

G

G

K

T

P

P

E

E

D

E

L

L

binding adenosine-5'-diphosphate: S39 (≠ N48), T41 (≠ V50), G42 (= G51), K43 (= K52), E140 (= E149), S142 (= S151), M145 (≠ Q154)

7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
27% identity, 66% coverage: 23:237/326 of query aligns to 14:228/263 of 7d08B

query
sites
7d08B

K
x
R

A

G

K

E

K
x
R

E

V

I

I

L

Y

S

D

N

N

L

I

D

S

F

L

T

N

L

I

F

R

S

R

G

G

E

Q

L

I

T

T

C

A

L

I

L

M

G

G

P

P

N

S

G
|
G

V
x
T

G

G

K
|
K

S
x
T

T
|
T

L

L

I

L

K

R

A

L

I

I

L

G

G

G

Q

Q

I

L

H

V

P

P

C

D

K

Q

G

G

S

E

I

V

Q

L

L

L

D

D

Q

G

R

K

S

D

I

I

T

A

D

Q

Y

M

S

S

I

R

E

Q

E

E

L

L

-

F

-

A

A

R

K

A

R

R

I

M

A

G

V

M

V

L

L

F

T

Q

D

S

P

G

V

A

F

L

P

F

G

T

N

D

M

M

T

S

V

V

G

Y

Q

E

L

N

V

V

A

A

L

F

G

P

R

I

T

R

P

A

H

H

T

T

G

K

W

L

S

S

G

E

K

N

L

L

N

I

P

A

A

-

D

-

R

-

D

E

V

L

V

V

E

A<