SitesBLAST
Comparing WP_111392228.1 NCBI__GCF_003253485.1:WP_111392228.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
50% identity, 99% coverage: 1:257/260 of query aligns to 11:267/267 of Q9LBG2
- 17:42 (vs. 7:32, 54% identical) binding NAD(+)
- E103 (= E93) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
1iy8A Crystal structure of levodione reductase (see paper)
50% identity, 99% coverage: 1:257/260 of query aligns to 2:258/258 of 1iy8A
- active site: G15 (= G14), S143 (= S142), Q153 (= Q152), Y156 (= Y155), K160 (= K159)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), S14 (≠ M13), G15 (= G14), L16 (= L15), D35 (= D34), V36 (≠ Y35), A62 (= A61), D63 (= D62), V64 (= V63), N90 (= N89), G92 (= G91), I93 (= I92), T141 (≠ V140), S143 (= S142), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (= G186), T191 (= T190), P192 (= P191), M193 (= M192)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
38% identity, 97% coverage: 1:252/260 of query aligns to 4:256/260 of 6zzqA
- active site: G17 (= G14), S142 (= S142), Y155 (= Y155)
- binding acetoacetic acid: Q94 (≠ E93), S142 (= S142), K152 (≠ Q152), Y155 (= Y155), Q196 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G13 (= G10), S16 (≠ M13), G17 (= G14), I18 (≠ L15), D37 (= D34), M38 (≠ Y35), D63 (= D62), V64 (= V63), N90 (= N89), A91 (= A90), G92 (= G91), M140 (≠ V140), A141 (= A141), S142 (= S142), Y155 (= Y155), K159 (= K159), Y187 (≠ A187), V188 (≠ I188), T190 (= T190)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
38% identity, 97% coverage: 1:252/260 of query aligns to 5:257/261 of 6zzsD
- active site: G18 (= G14), S143 (= S142), Y156 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G14 (= G10), S17 (≠ M13), I19 (≠ L15), D38 (= D34), M39 (≠ Y35), D64 (= D62), V65 (= V63), N91 (= N89), A92 (= A90), G93 (= G91), M141 (≠ V140), A142 (= A141), S143 (= S142), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (= G186), V189 (≠ I188), T191 (= T190), L193 (≠ M192)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ E93), S143 (= S142), N145 (≠ G144), K153 (≠ Q152), Y156 (= Y155), Q197 (vs. gap)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
39% identity, 97% coverage: 1:252/260 of query aligns to 3:244/248 of 6ixmC
- active site: G16 (= G14), S142 (= S142), Y155 (= Y155), K159 (= K159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G10), S15 (≠ M13), G16 (= G14), I17 (≠ L15), D36 (= D34), I37 (≠ Y35), A61 (= A61), D62 (= D62), T63 (≠ V63), N89 (= N89), A90 (= A90), M140 (≠ V140), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), A186 (≠ G186), Y187 (≠ A187), I188 (= I188), L192 (≠ M192)
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
37% identity, 98% coverage: 1:254/260 of query aligns to 2:258/260 of 5b4tA
- active site: G15 (= G14), N114 (= N114), S142 (= S142), Y155 (= Y155), K159 (= K159), I200 (≠ F197)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ E93), S142 (= S142), H144 (≠ G144), K152 (≠ Q152), Y155 (= Y155), W187 (≠ A187), Q196 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ A12), G15 (= G14), I16 (≠ L15), F36 (≠ Y35), D63 (= D62), L64 (≠ V63), N90 (= N89), G92 (= G91), L113 (≠ I113), I140 (≠ V140), Y155 (= Y155), K159 (= K159), P185 (= P185), G186 (= G186), W187 (≠ A187), V188 (≠ I188), T190 (= T190), L192 (≠ M192), V193 (= V193)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
37% identity, 98% coverage: 1:254/260 of query aligns to 2:258/260 of 3w8dA
- active site: G15 (= G14), N114 (= N114), S142 (= S142), Y155 (= Y155), K159 (= K159), I200 (≠ F197)
- binding methylmalonic acid: Q94 (≠ E93), S142 (= S142), H144 (≠ G144), K152 (≠ Q152), Y155 (= Y155), W187 (≠ A187), Q196 (vs. gap), W257 (≠ E253)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ A12), S14 (≠ M13), G15 (= G14), I16 (≠ L15), F36 (≠ Y35), A62 (= A61), D63 (= D62), L64 (≠ V63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I113), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), G186 (= G186), W187 (≠ A187), V188 (≠ I188), T190 (= T190), L192 (≠ M192), V193 (= V193)
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
37% identity, 98% coverage: 1:254/260 of query aligns to 2:258/260 of 3vdrA
- active site: G15 (= G14), N114 (= N114), S142 (= S142), Y155 (= Y155), K159 (= K159), I200 (≠ F197)
- binding (3R)-3-hydroxybutanoic acid: Q94 (≠ E93), H144 (≠ G144), K152 (≠ Q152), Y155 (= Y155), W187 (≠ A187), Q196 (vs. gap), W257 (≠ E253)
- binding acetoacetic acid: Q94 (≠ E93), H144 (≠ G144), K152 (≠ Q152), Y155 (= Y155), W187 (≠ A187), Q196 (vs. gap), W257 (≠ E253)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (≠ A12), I16 (≠ L15), F36 (≠ Y35), D63 (= D62), L64 (≠ V63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I113), K159 (= K159), G186 (= G186), V188 (≠ I188), T190 (= T190), L192 (≠ M192), V193 (= V193)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G10), T13 (≠ A12), I16 (≠ L15), F36 (≠ Y35), D63 (= D62), L64 (≠ V63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I113), S142 (= S142), Y155 (= Y155), K159 (= K159), G186 (= G186), V188 (≠ I188), T190 (= T190), L192 (≠ M192), V193 (= V193)
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
37% identity, 98% coverage: 1:254/260 of query aligns to 2:258/260 of 3vdqA
- active site: G15 (= G14), N114 (= N114), S142 (= S142), Y155 (= Y155), K159 (= K159), I200 (≠ F197)
- binding acetate ion: Q94 (≠ E93), H144 (≠ G144), K152 (≠ Q152), W187 (≠ A187), L192 (≠ M192), Q196 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), S14 (≠ M13), I16 (≠ L15), F36 (≠ Y35), D63 (= D62), L64 (≠ V63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I113), I140 (≠ V140), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), G186 (= G186), W187 (≠ A187), V188 (≠ I188), T190 (= T190), L192 (≠ M192), V193 (= V193)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
40% identity, 97% coverage: 1:252/260 of query aligns to 5:241/244 of 4nbuB
- active site: G18 (= G14), N111 (= N114), S139 (= S142), Q149 (= Q152), Y152 (= Y155), K156 (= K159)
- binding acetoacetyl-coenzyme a: D93 (≠ Q96), K98 (≠ E101), S139 (= S142), N146 (≠ V149), V147 (≠ L150), Q149 (= Q152), Y152 (= Y155), F184 (≠ A187), M189 (= M192), K200 (≠ E211)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G10), N17 (≠ M13), G18 (= G14), I19 (≠ L15), D38 (= D34), F39 (≠ Y35), V59 (= V59), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), I90 (= I92), T137 (≠ V140), S139 (= S142), Y152 (= Y155), K156 (= K159), P182 (= P185), F184 (≠ A187), T185 (≠ I188), T187 (= T190), M189 (= M192)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
36% identity, 98% coverage: 1:254/260 of query aligns to 2:258/260 of 2ztlA
- active site: G15 (= G14), N114 (= N114), S142 (= S142), Y155 (= Y155), K159 (= K159), L200 (≠ F197)
- binding (3s)-3-hydroxybutanoic acid: Q94 (≠ E93), S142 (= S142), H144 (≠ G144), K152 (≠ Q152), Y155 (= Y155), Q196 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), G15 (= G14), I16 (≠ L15), F36 (≠ Y35), L64 (≠ V63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ I113), Y155 (= Y155), K159 (= K159), P185 (= P185), W187 (≠ A187), V188 (≠ I188), T190 (= T190), V193 (= V193)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
36% identity, 98% coverage: 1:254/260 of query aligns to 2:258/260 of 1wmbA
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme
39% identity, 97% coverage: 1:252/260 of query aligns to 4:245/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (= G10), S16 (≠ M13), G17 (= G14), I18 (≠ L15), D37 (= D34), I38 (≠ Y35), A62 (= A61), D63 (= D62), S64 (≠ V63), N90 (= N89), M141 (≠ V140), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (= G186), Y188 (≠ A187), I189 (= I188), L193 (≠ M192)
4fn4A Short-chain NAD(h)-dependent dehydrogenase/reductase from sulfolobus acidocaldarius (see paper)
36% identity, 97% coverage: 1:252/260 of query aligns to 5:250/254 of 4fn4A
- active site: G18 (= G14), S144 (= S142), Y157 (= Y155), K161 (= K159), S202 (= S210)
- binding nicotinamide-adenine-dinucleotide: G14 (= G10), S17 (≠ M13), G18 (= G14), I19 (≠ L15), E38 (≠ D34), L39 (≠ Y35), R43 (≠ A43), A63 (= A61), D64 (= D62), V65 (= V63), N91 (= N89), G93 (= G91), I94 (= I92), T142 (≠ V140), S144 (= S142), Y157 (= Y155), K161 (= K159), P187 (= P185), V190 (≠ I188), T192 (= T190), N193 (= N201), I194 (≠ P202)
1zemA Crystal structure of NAD+-bound xylitol dehydrogenase (see paper)
35% identity, 96% coverage: 4:252/260 of query aligns to 6:260/260 of 1zemA
- active site: N16 (≠ G14), S142 (= S142), Y155 (= Y155), K159 (= K159), D212 (= D204)
- binding nicotinamide-adenine-dinucleotide: G12 (= G10), G15 (≠ M13), N16 (≠ G14), I17 (≠ L15), D36 (= D34), M37 (≠ Y35), D62 (= D62), V63 (= V63), N89 (= N89), A90 (= A90), G91 (= G91), T140 (≠ V140), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), M188 (≠ I188)
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
36% identity, 99% coverage: 1:258/260 of query aligns to 2:247/247 of 7pcsB