SitesBLAST

Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
40% identity, 97% coverage: 3:150/152 of query aligns to 5:153/157 of Q0QLF3

query
sites
Q0QLF3

T

T

F

I

N

N

L

L

T

N

I

L

N

N

G

G

T

E

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A

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C
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C

G

G

A

A

C
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C

T

T

I

V

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I

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F

D

N

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G

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D

A

P

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V

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T

C
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C

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C

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M

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I

M

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N

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D

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C

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A

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Y

A

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E

C42 (= C40) binding [2Fe-2S] cluster
C47 (= C45) binding [2Fe-2S] cluster
C50 (= C48) binding [2Fe-2S] cluster
C62 (= C60) binding [2Fe-2S] cluster
C101 (= C98) binding [2Fe-2S] cluster
C104 (= C101) binding [2Fe-2S] cluster
C136 (= C133) binding [2Fe-2S] cluster
C138 (= C135) binding [2Fe-2S] cluster

1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
40% identity, 97% coverage: 6:152/152 of query aligns to 6:153/155 of 1ffvA

query
sites
1ffvA

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N

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x
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C

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x
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C

G
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G

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C

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D

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E

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M

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C

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G

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Y

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binding fe2/s2 (inorganic) cluster: I38 (≠ F38), C40 (= C40), S43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), C60 (= C60), C99 (= C98), G100 (= G99), C102 (= C101), C134 (= C133), C136 (= C135)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q97), C136 (= C135)

1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
39% identity, 98% coverage: 4:152/152 of query aligns to 5:154/156 of 1ffuD

query
sites
1ffuD

F

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N

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G

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E

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C

G

E

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T

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x
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x
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C

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G

A

A

C
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C

T

T

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C

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G

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N

N

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M

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E

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N

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P

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N

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E

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M

A

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M

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C

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binding fe2/s2 (inorganic) cluster: C41 (= C40), S44 (≠ A43), H45 (≠ Q44), C46 (= C45), G47 (= G46), C49 (= C48), C61 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C135 (= C133), C137 (= C135)

P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
41% identity, 96% coverage: 4:149/152 of query aligns to 63:224/229 of P77165

query
sites
P77165

F

L

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L

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N

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L

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K

K

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A

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Q

Q

C
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C

G
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G

A

A

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C

T

T

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L

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N

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N

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C99 (= C40) binding [2Fe-2S] cluster
C104 (= C45) binding [2Fe-2S] cluster
G105 (= G46) binding [2Fe-2S] cluster
C107 (= C48) binding [2Fe-2S] cluster
C119 (= C60) binding [2Fe-2S] cluster
C158 (= C98) binding [2Fe-2S] cluster
C161 (= C101) binding [2Fe-2S] cluster
C208 (= C133) binding [2Fe-2S] cluster
C210 (= C135) binding [2Fe-2S] cluster

5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
41% identity, 96% coverage: 4:149/152 of query aligns to 12:173/175 of 5g5gA

query
sites
5g5gA

F

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G

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K

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C

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x
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x
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C

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L

L

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N

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L

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D

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binding fe2/s2 (inorganic) cluster: G47 (= G39), C48 (= C40), D49 (≠ G41), G51 (≠ A43), C53 (= C45), G54 (= G46), C56 (= C48), C68 (= C60), C107 (= C98), G108 (= G99), C110 (= C101), C157 (= C133), C159 (= C135)

1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
44% identity, 100% coverage: 1:152/152 of query aligns to 1:156/906 of 1dgjA

query
sites
1dgjA

M

M

A

E

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T

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T

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N

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L

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K

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x
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C

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G

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K

A
x
G

Q
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Q

C
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C

G
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G

A

A

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C

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T

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Q

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C

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G

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C
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G

Q

F

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I

M

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A

A

A

K

A

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L

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D

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E

N

N

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E

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D

I

V

E

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N

D

A

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M

F

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H

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N

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C
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C
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C

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T

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Y

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A

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A

N

I

L

L

binding fe2/s2 (inorganic) cluster: V38 (≠ F38), G39 (= G39), C40 (= C40), G41 (= G41), G43 (≠ A43), Q44 (= Q44), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C137 (= C133), C139 (= C135)
binding pterin cytosine dinucleotide: Q99 (= Q97), C139 (= C135)

Sites not aligning to the query:

active site: 391, 427, 503, 507, 535, 869, 870
binding molybdenum (iv)oxide: 424, 535, 698, 869
binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869

1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
41% identity, 99% coverage: 2:152/152 of query aligns to 2:154/161 of 1n5wA

query
sites
1n5wA

A

A

T

H

F

I

N

E

L

L

T

T

I

I

N

N

G

G

T

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S

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D

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x
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G

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C

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D

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x
S

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x
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C

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G

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A

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C

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V

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L

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D

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G

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C

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A

Q

A

A

E

N

G

G

A

A

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S

I

I

T

T

I

I

E

E

G

G

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M

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A

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A

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P

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N

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E

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C

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G

Y

Y

C
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C

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M

I

I

M

M

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A

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A

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L

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E

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E

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I

binding flavin-adenine dinucleotide: S43 (≠ A43), H44 (≠ Q44)
binding fe2/s2 (inorganic) cluster: I38 (≠ F38), G39 (= G39), C40 (= C40), S43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C135 (= C133), C137 (= C135)

P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
41% identity, 99% coverage: 2:152/152 of query aligns to 4:156/166 of P19921

query
sites
P19921

A

A

T

H

F

I

N

E

L

L

T

T

I

I

N

N

G

G

T

H

S

P

K

V

Q

E

V

A

D

L

V

V

D

E

P

P

S

R

T

T

P

L

V

L

L

I

W

H

V

F

L

I

R

R

D

E

H

Q

L

Q

D

N

L

L

V

T

G

G

T

A

K

H

F

I

G

G

C
|
C

G

D

I

T

A

S

Q

H

C
|
C

G

G

A

A

C
|
C

T

T

I

V

H

D

L

L

D

D

G

G

N

M

A

S

V

V

R

K

S

S

C
|
C

Q

T

L

M

P

F

V

A

S

V

A

Q

A

A

E

N

G

G

A

A

S

S

I

I

T

T

I

I

E

E

G

G

L

M

S

A

-

A

-

P

E

D

N

G

G

T

D

L

H

S

P

A

V

L

Q

Q

K

E

A

G

W

F

M

R

E

M

H

M

D

H

V

G

P

L

Q

Q

C
|
C

G

G

Y

Y

C
|
C

Q

T

A

P

G

G

Q

M

I

I

M

M

T

R

A

S

A

H

A

R

L

L

Q

Q

Q

E

N

N

P

P

N

S

P

P

S

T

E

E

E

A

D

E

I

I

E

R

N

F

A

G

M

I

N

G

G

G

N

N

I

L

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

Q

R

N

I

I

K

V

A

K

A

A

V

I

K

Q

T

Y

A

A

Q

A

N

K

L

I

C42 (= C40) binding [2Fe-2S] cluster
C47 (= C45) binding [2Fe-2S] cluster
C50 (= C48) binding [2Fe-2S] cluster
C62 (= C60) binding [2Fe-2S] cluster
C102 (= C98) binding [2Fe-2S] cluster
C105 (= C101) binding [2Fe-2S] cluster
C137 (= C133) binding [2Fe-2S] cluster
C139 (= C135) binding [2Fe-2S] cluster

1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
41% identity, 99% coverage: 2:152/152 of query aligns to 2:154/158 of 1n5wD

query
sites
1n5wD

A

A

T

H

F

I

N

E

L

L

T

T

I

I

N

N

G

G

T

H

S

P

K

V

Q

E

V

A

D

L

V

V

D

E

P

P

S

R

T

T

P

L

V

L

L

I

W

H

V

F

L

I

R

R

D

E

H

Q

L

Q

D

N

L

L

V

T

G

G

T

A

K

H

F

I

G

G

C
|
C

G

D

I

T

A
x
S

Q
x
H

C
|
C

G
|
G

A

A

C
|
C

T

T

I

V

H

D

L

L

D

D

G

G

N

M

A

S

V

V

R

K

S

S

C
|
C

Q

T

L

M

P

F

V

A

S

V

A

Q

A

A

E

N

G

G

A

A

S

S

I

I

T

T

I

I

E

E

G

G

L

M

S

A

-

A

-

P

E

D

N

G

G

T

D

L

H

S

P

A

V

L

Q

Q

K

E

A

G

W

F

M

R

E

M

H

M

D

H

V

G

P

L

Q
|
Q

C
|
C

G
|
G

Y

Y

C
|
C

Q

T

A

P

G

G

Q

M

I

I

M

M

T

R

A

S

A

H

A

R

L

L

Q

Q

Q

E

N

N

P

P

N

S

P

P

S

T

E

E

E

A

D

E

I

I

E

R

N

F

A

G

M

I

N

G

G

G

N

N

I

L

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

Q

R

N

I

I

K

V

A

K

A

A

V

I

K

Q

T

Y

A

A

Q

A

N

K

L

I

binding flavin-adenine dinucleotide: S43 (≠ A43), H44 (≠ Q44)
binding fe2/s2 (inorganic) cluster: C40 (= C40), S43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C135 (= C133), C137 (= C135)
binding pterin cytosine dinucleotide: Q99 (= Q97), C137 (= C135)

1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
40% identity, 95% coverage: 1:145/152 of query aligns to 3:148/162 of 1t3qA

query
sites
1t3qA

M

L

A

M

T

R

F

I

N

S

L

A

T

T

I

I

N

N

G

G

T

K

S

P

K

R

Q

V

V

F

D

Y

V

V

D

E

P

P

S

R

T

M

P

H

V

L

L

A

W

D

V

A

L

L

R

R

D

E

H

V

L

V

D

G

L

L

V

T

G

G

T

T

K

K

F
x
I

G

G

C
|
C

G
x
E

I

Q

A
x
G

Q

V

C
|
C

G
|
G

A

S

C
|
C

T

T

I

I

H

L

L

I

D

D

G

G

N

A

A

P

V

M

R
|
R

S

S

C
|
C

Q

L

L

T

P

L

V

A

S

V

A

Q

A

A

E

E

G

G

A

C

S

S

I

I

T

E

T

T

I

V

E

E

G

G

L

L

S

S

E

Q

N

G

G

E

D

K

-

L

H

N

P

A

V

L

Q

Q

K

D

A

S

W

F

M

R

E

R

H

H

D

H

V

A

P

L

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

Q

T

A

A

G

G

Q

M

I

L

M

A

T

T

A

A

A

R

A

S

L

I

L

L

Q

A

Q

E

N

N

P

P

N

A

P

P

S

S

E

R

E

D

D

E

I

V

E

R

N

E

A

V

M

M

N

S

G

G

N

N

I

L

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

E

R

T

I

I

K

I

A

D

A

A

V

I

binding fe2/s2 (inorganic) cluster: I40 (≠ F38), C42 (= C40), E43 (≠ G41), G45 (≠ A43), C47 (= C45), G48 (= G46), C50 (= C48), R60 (= R58), C62 (= C60), C101 (= C98), G102 (= G99), C104 (= C101), C136 (= C133), C138 (= C135)
binding pterin cytosine dinucleotide: Q100 (= Q97), C138 (= C135)

4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
43% identity, 95% coverage: 6:149/152 of query aligns to 6:153/907 of 4usaA

query
sites
4usaA

L

I

T

T

I

V

N

N

G

G

T

I

S

E

K

Q

Q

N

V

L

D

F

V

V

D

D

P

A

S

E

T

A

P

L

V

L

L

S

W

D

V

V

L

L

R

R

D

Q

H

Q

L

L

D

G

L

L

V

T

G

G

T

V

K

K

F
x
V

G

G

C
|
C

G
x
E

I

Q

A
x
G

Q

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

I

V

H

I

L

L

D

D

G

G

N

K

A

V

V

V

R
|
R

S

A

C
|
C

Q

V

L

T

P

K

V

M

S

K

-

R

A

V

A

A

E

D

G

G

A

A

S

Q

I

I

T

T

I

I

E

E

G

G

L

V

S

G

E

Q

N

P

G

E

D

N

-

L

H

H

P

P

V

L

Q

Q

K

K

A

A

W

W

M

V

E

L

H

H

D

G

V

G

P

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

Q

S

A

P

G

G

Q

F

I

I

M

V

T

S

A

A

A

K

A

G

L

L

Q

D

Q

T

N

N

P

A

N

D

P

P

S

S

E

R

E

E

D

D

I

V

E

R

N

D

A

W

M

F

-

Q

-

K

N

H

G

R

N

N

I

A

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

K

R

P

I

L

K

V

A

D

A

A

V

V

K

M

T

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ F38), C40 (= C40), E41 (≠ G41), G43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C137 (= C133), C139 (= C135)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q97), C139 (= C135)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 531, 532, 535, 539
binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
43% identity, 95% coverage: 6:149/152 of query aligns to 6:153/907 of 4us9A

query
sites
4us9A

L

I

T

T

I

V

N

N

G

G

T

I

S

E

K

Q

Q

N

V

L

D

F

V

V

D

D

P

A

S

E

T

A

P

L

V

L

L

S

W

D

V

V

L

L

R

R

D

Q

H

Q

L

L

D

G

L

L

V

T

G

G

T

V

K

K

F
x
V

G

G

C
|
C

G
x
E

I

Q

A
x
G

Q

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

I

V

H

I

L

L

D

D

G

G

N

K

A

V

V

V

R
|
R

S

A

C
|
C

Q

V

L

T

P

K

V

M

S

K

-

R

A

V

A

A

E

D

G

G

A

A

S

Q

I

I

T

T

I

I

E

E

G

G

L

V

S

G

E

Q

N

P

G

E

D

N

-

L

H

H

P

P

V

L

Q

Q

K

K

A

A

W

W

M

V

E

L

H

H

D

G

V

G

P

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

Q

S

A

P

G

G

Q

F

I

I

M

V

T

S

A

A

A

K

A

G

L

L

Q

D

Q

T

N

N

P

A

N

D

P

P

S

S

E

R

E

E

D

D

I

V

E

R

N

D

A

W

M

F

-

Q

-

K

N

H

G

R

N

N

I

A

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

K

R

P

I

L

K

V

A

D

A

A

V

V

K

M

T

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ F38), C40 (= C40), E41 (≠ G41), G43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C137 (= C133), C139 (= C135)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q97), C139 (= C135)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding 3-phenylpropanal: 255, 257, 258, 752
binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
43% identity, 95% coverage: 6:149/152 of query aligns to 6:153/907 of 4us8A

query
sites
4us8A

L

I

T

T

I

V

N

N

G

G

T

I

S

E

K

Q

Q

N

V

L

D

F

V

V

D

D

P

A

S

E

T

A

P

L

V

L

L

S

W

D

V

V

L

L

R

R

D

Q

H

Q

L

L

D

G

L

L

V

T

G

G

T

V

K

K

F
x
V

G

G

C
|
C

G
x
E

I

Q

A
x
G

Q

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

I

V

H

I

L

L

D

D

G

G

N

K

A

V

V

V

R
|
R

S

A

C
|
C

Q

V

L

T

P

K

V

M

S

K

-

R

A

V

A

A

E

D

G

G

A

A

S

Q

I

I

T

T

I

I

E

E

G

G

L

V

S

G

E

Q

N

P

G

E

D

N

-

L

H

H

P

P

V

L

Q

Q

K

K

A

A

W

W

M

V

E

L

H

H

D

G

V

G

P

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

Q

S

A

P

G

G

Q

F

I

I

M

V

T

S

A

A

A

K

A

G

L

L

Q

D

Q

T

N

N

P

A

N

D

P

P

S

S

E

R

E

E

D

D

I

V

E

R

N

D

A

W

M

F

-

Q

-

K

N

H

G

R

N

N

I

A

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

K

R

P

I

L

K

V

A

D

A

A

V

V

K

M

T

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ F38), C40 (= C40), E41 (≠ G41), G43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C137 (= C133), C139 (= C135)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q97), C139 (= C135)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 498, 531, 532, 535, 539
binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
43% identity, 95% coverage: 6:149/152 of query aligns to 6:153/907 of 4c7yA

query
sites
4c7yA

L

I

T

T

I

V

N

N

G

G

T

I

S

E

K

Q

Q

N

V

L

D

F

V

V

D

D

P

A

S

E

T

A

P

L

V

L

L

S

W

D

V

V

L

L

R

R

D

Q

H

Q

L

L

D

G

L

L

V

T

G

G

T

V

K

K

F

V

G

G

C
|
C

G
x
E

I

Q

A
x
G

Q

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

I

V

H

I

L

L

D

D

G

G

N

K

A

V

V

V

R
|
R

S

A

C
|
C

Q

V

L

T

P

K

V

M

S

K

-

R

A

V

A

A

E

D

G

G

A

A

S

Q

I

I

T

T

I

I

E

E

G

G

L

V

S

G

E

Q

N

P

G

E

D

N

-

L

H

H

P

P

V

L

Q

Q

K

K

A

A

W

W

M

V

E

L

H

H

D

G

V

G

P

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

Q

S

A

P

G

G

Q

F

I

I

M

V

T

S

A

A

A

K

A

G

L

L

Q

D

Q

T

N

N

P

A

N

D

P

P

S

S

E

R

E

E

D

D

I

V

E

R

N

D

A

W

M

F

-

Q

-

K

N

H

G

R

N

N

I

A

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

K

R

P

I

L

K

V

A

D

A

A

V

V

K

M

T

D

A

A

binding fe2/s2 (inorganic) cluster: C40 (= C40), E41 (≠ G41), G43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C137 (= C133), C139 (= C135)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q97), C139 (= C135)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding bicarbonate ion: 460, 498, 531, 535, 539
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
binding hydrogen peroxide: 696, 697, 869

3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
43% identity, 95% coverage: 6:149/152 of query aligns to 6:153/907 of 3fc4A

query
sites
3fc4A

L

I

T

T

I

V

N

N

G

G

T

I

S

E

K

Q

Q

N

V

L

D

F

V

V

D

D

P

A

S

E

T

A

P

L

V

L

L

S

W

D

V

V

L

L

R

R

D

Q

H

Q

L

L

D

G

L

L

V

T

G

G

T

V

K

K

F
x
V

G

G

C
|
C

G
x
E

I

Q

A
x
G

Q

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

I

V

H

I

L

L

D

D

G

G

N

K

A

V

V

V

R
|
R

S

A

C
|
C

Q

V

L

T

P

K

V

M

S

K

-

R

A

V

A

A

E

D

G

G

A

A

S

Q

I

I

T

T

I

I

E

E

G

G

L

V

S

G

E

Q

N

P

G

E

D

N

-

L

H

H

P

P

V

L

Q

Q

K

K

A

A

W

W

M

V

E

L

H

H

D

G

V

G

P

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

Q

S

A

P

G

G

Q

F

I

I

M

V

T

S

A

A

A

K

A

G

L

L

Q

D

Q

T

N

N

P

A

N

D

P

P

S

S

E

R

E

E

D

D

I

V

E

R

N

D

A

W

M

F

-

Q

-

K

N

H

G

R

N

N

I

A

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

K

R

P

I

L

K

V

A

D

A

A

V

V

K

M

T

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ F38), C40 (= C40), E41 (≠ G41), G43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C137 (= C133), C139 (= C135)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q97), C139 (= C135)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding 1,2-ethanediol: 535, 622, 696, 697, 869
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
43% identity, 95% coverage: 6:149/152 of query aligns to 6:153/907 of 3fahA

query
sites
3fahA

L

I

T

T

I

V

N

N

G

G

T

I

S

E

K

Q

Q

N

V

L

D

F

V

V

D

D

P

A

S

E

T

A

P

L

V

L

L

S

W

D

V

V

L

L

R

R

D

Q

H

Q

L

L

D

G

L

L

V

T

G

G

T

V

K

K

F
x
V

G

G

C
|
C

G
x
E

I

Q

A
x
G

Q

Q

C
|
C

G
|
G

A

A

C
|
C

T

S

I

V

H

I

L

L

D

D

G

G

N

K

A

V

V

V

R
|
R

S

A

C
|
C

Q

V

L

T

P

K

V

M

S

K

-

R

A

V

A

A

E

D

G

G

A

A

S

Q

I

I

T

T

I

I

E

E

G

G

L

V

S

G

E

Q

N

P

G

E

D

N

-

L

H

H

P

P

V

L

Q

Q

K

K

A

A

W

W

M

V

E

L

H

H

D

G

V

G

P

A

Q
|
Q

C
|
C

G
|
G

Y

F

C
|
C

Q

S

A

P

G

G

Q

F

I

I

M

V

T

S

A

A

A

K

A

G

L

L

Q

D

Q

T

N

N

P

A

N

D

P

P

S

S

E

R

E

E

D

D

I

V

E

R

N

D

A

W

M

F

-

Q

-

K

N

H

G

R

N

N

I

A

C
|
C

R

R

C
|
C

G

T

T

G

Y

Y

L

K

R

P

I

L

K

V

A

D

A

A

V

V

K

M

T

D

A

A

binding fe2/s2 (inorganic) cluster: V38 (≠ F38), C40 (= C40), E41 (≠ G41), G43 (≠ A43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C98), G101 (= G99), C103 (= C101), C137 (= C133), C139 (= C135)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q97), C139 (= C135)

Sites not aligning to the query:

active site: 390, 425, 501, 505, 533, 869, 870
binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
binding magnesium ion: 899, 903
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869

Query Sequence

>WP_111393333.1 NCBI__GCF_003253485.1:WP_111393333.1
MATFNLTINGTSKQVDVDPSTPVLWVLRDHLDLVGTKFGCGIAQCGACTIHLDGNAVRSC
QLPVSAAEGASITTIEGLSENGDHPVQKAWMEHDVPQCGYCQAGQIMTAAALLQQNPNPS
EEDIENAMNGNICRCGTYLRIKAAVKTAAQNL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory