SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_111605592.1 NCBI__GCF_003259225.1:WP_111605592.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P31120 Phosphoglucosamine mutase; EC 5.4.2.10 from Escherichia coli (strain K12) (see 3 papers)
60% identity, 98% coverage: 2:441/448 of query aligns to 4:445/445 of P31120

query
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P31120

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S100 (= S97) mutation to A: 2% of wild-type activity.; mutation to T: 20-fold increase in the non-specific phosphoglucomutase activity towards glucose-phosphate substrates (non aminated).
S102 (= S99) active site, Phosphoserine intermediate; modified: Phosphoserine; by autocatalysis; mutation to A: Loss of activity in the absence or presence of glucosamine-1,6-diP.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7omlA Bacillus subtilis phosphoglucomutase glmm (metal bound) (see paper)
43% identity, 98% coverage: 3:440/448 of query aligns to 2:442/445 of 7omlA

query
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7omlA

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binding magnesium ion: S99 (= S99), D239 (= D238), D241 (= D240), D243 (= D242)

7ojrA Bacillus subtilis phosphoglucomutase glmm (phosphate bound) (see paper)
43% identity, 98% coverage: 3:440/448 of query aligns to 2:442/445 of 7ojrA

query
sites
7ojrA

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A

S

V

E

A

V

N

E

E

K

R

E

L

M

N

N

G

G

L

D

G

G

R

R

V

I

L

L

L

V

R
|
R

A

P

S
|
S

G

G

T
|
T

E

E

P

P

L

L

I

V

R
|
R

V

V

M

M

V

A

E

E

G

A

R

K

D

T

D

K

Q

E

T

L

V

C

D

D

E

E

L

Y

V

V

D

N

Y

R

L

I

V

V

E

E

E

V

V

V

N

R

S

S

binding phosphate ion: R409 (= R407), S411 (= S409), T413 (= T411), R418 (= R416)

3i3wA Structure of a phosphoglucosamine mutase from francisella tularensis
43% identity, 97% coverage: 3:438/448 of query aligns to 1:436/441 of 3i3wA

query
sites
3i3wA

K

K

Y

Y

F

F

G

G

T

T

D

D

G

G

I

I

R
|
R

G

G

K

E

V

V

G

A

T

N

M

S

P

T

I

I

T

T

P

V

E

E

F

F

M

T

L

Q

K

K

L

L

G

G

W

N

A

A

A

V

G

G

Q

S

V

L

F

I

K

N

E

Q

N

K

D

N

-

Y

-

P

K

K

K

F

I

V

L

I

I

V

G

G

K

Q

D

D

T

T

R

R

I

S

S

S

G

G

Y

G

M

F

F

L

E

K

S

F

A

A

L

L

E

V

S

S

G

G

I

L

V

N

A

A

A

A

G

G

A

I

D

D

V

V

R

L

L

D

V

L

G

G

P

V

M

V

P

P

T

T

P

P

A

V

I

V

A

A

Y

F

L

M

T

T

R

V

T

K

F

H

R

R

A

A

S

A

A

A

G

G

I

F

V

V

I

I

S

T

A

A

S
|
S

H
|
H

N

N

P

K

Y

F

T

T

D

D

N

N

G

G

I

I

K
|
K

F

L

F

F

S

S

A

S

E

N

G

G

G

F

K

K

I

L

S

D

D

D

E

A

I

L

E

E

E

E

R

E

I

V

E

E

Y

D

F

M

L

I

E

D

Q

G

P

D

M

F

V

I

V

Y

V

Q

E

P

S

Q

S

F

L

K

I

F

G

G

R

S

A

Y

K

K

R

I

I

L

D

A

D

N

A

A

A

I

G

D

R

E

Y

Y

I

I

E

E

Y

S

C

I

K

Y

G

S

T

R

F

F

P

A

I

K

G

F

L

V

Q

N

L

Y

S

K

G

G

L

-

K

K

I

V

V

V

V

V

D

D

C

C

A

A

D

H

G

G

A

A

T

A

Y

S

H

H

V

N

A

F

P

E

R

A

V

L

F

L

S

D

E

K

L

F

G

G

A

I

E

N

V

Y

I

V

S

S

I

I

G

A

V

S

N

N

P

P

D

D

G

G

L

L

N

N

I

I

N

N

E

V

F

G

S

C

G

G

A

A

T

T

K

C

P

V

E

S

L

N

L

I

R

K

K

K

N

A

V

V

L

K

A

E

E

Q

E

K

A

A

D

D

I

L

G

G

I

I

A

S

L

L

D
|
D

G

G

D
|
D

G

A

D
|
D

R
|
R

L

I

I

I

L

I

V

V

D

D

R

E

H

N

G

G

E

Q

I

E

R

I

D

D

G

G

D

D

D

G

I

I

L

L

Y

N

I

I

I

L

A

A

N

Q

H

Y

L

S

M

D

R

I

T

C

G

G

R

G

F

-

S

T

G

N

G

G

V

I

V

V

G

G

T

T

L

Q

M

M

S

T

N

N

F

M

G

S

L

Y

E

E

L

N

A

H

F

Y

S

R

E

A

T

N

G

K

I

I

G

P

F

F

S

I

R

R

A

S

A

K

V

V

G

G

D

D

R

R

Y

Y

V

V

N

L

E

E

K

D

L

L

M

V

Q

K

H

Y

G

G

W

Y

V

K

L

I

G

G

G

G

E

E

P

S

S

S

G

G

H
|
H

I

V

V

I

C

N

R

L

S

N

I

F

T

G

T

T

T

T

G

G

D

D

G

G

I

L

I

F

A

T

A

A

L

I

Q

Q

V

L

L

L

R

A

A

I

M

F

V

S

E

Q

E

A

G

D

K

K

S

P

L

V

D

S

E

E

L

F

L

K

V

L

G

Q

L

G

V

E

K

L

F

M

P

Q

Q

Q

K

T

L

L

K

I

N

N

I

V

R

P

V

L

A

T

K

K

R

K

F

-

V

V

P

A

N

R

E

E

E

D

P

-

A

-

L

L

Q

Q

K

K

A

V

I

A

A

S

V

D

A

V

N

N

E

D

-

V

-

E

-

K

R

R

L

L

N

G

G

N

L

R

G

G

R

R

V

V

L

L

L

L

R

R

A

P

S

S

G

G

T

T

E

E

P

P

L

V

I

L

R

R

V

V

M

M

V

V

E

E

G

A

R

D

D

D

D

K

Q

S

T

L

V

A

D

T

E

N

L

E

V

A

D

E

Y

Y

L

L

V

V

E

E

E

K

V

V

active site: R9 (= R11), S99 (= S99), H100 (= H100), K109 (= K109), D237 (= D238), D239 (= D240), D241 (= D242), R242 (= R243), H324 (= H326)
binding zinc ion: S99 (= S99), D237 (= D238), D239 (= D240), D241 (= D242)

1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
32% identity, 99% coverage: 1:443/448 of query aligns to 1:455/455 of 1wqaA

query
sites
1wqaA

M

M

R

G

K

K

Y

L

F

F

G

G

T

T

D

F

G

G

I

V

R
|
R

G

G

K

-

V

I

G

A

T

N

M

E

P

K

I

I

T

T

P

P

E

E

F

F

M

A

L

M

K

K

L

I

G

G

W

M

A

A

A

F

G

G

Q

T

V

L

F

L

K

K

E

R

N

E

D

G

K

R

K

K

-

K

-

P

-

L

I

V

L

V

I

V

G

G

K

R

D

D

T

T

R

R

I

V

S

S

G

G

Y

E

M

M

F

L

E

K

S

E

A

A

L

L

E

I

S

S

G

G

I

L

V

L

A

S

A

V

G

G

A

C

D

D

V

V

R

I

L

D

V

V

G

G

P

I

M

A

P

P

T

T

P

P

A

A

I

V

A

Q

Y

W

L

A

T

T

R

K

T

H

F

F

R

N

A

A

S

D

A

G

G

G

I

A

V

V

I

I

S

T

A

A

S
|
S

H
|
H

N

N

P

P

Y

P

T

E

D

Y

N

N

G

G

I

I

K
|
K

F

L

F

L

S

E

A

P

E

N

G

G

G

M

K

G

I

L

S

K

D

K

E

E

I

R

E

E

E

A

R

I

I

V

-

E

E

E

Y

L

F

F

L

F

E

K

Q

E

P

D

M

F

V

D

V

R

V

A

E

K

S

W

S

Y

L

E

I

I

G

G

R

E

A

V

K

R

R

R

I

-

D

E

D

D

A

I

A

I

G

K

R

P

Y

Y

I

I

E

E

Y

A

C

I

K

K

G

S

T

K

F

V

P

D

I

V

-

E

G

A

L

I

Q

K

L

K

S

R

G

K

L

P

K

F

I

V

V

V

V

V

D

D

C

T

A

S

D

N

G

G

A

A

T

G

Y

S

H

L

V

T

A

L

P

P

R

Y

V

L

F

L

S

R

E

E

L

L

G

G

A

C

E

K

V

V

I

I

S

T

I

V

G

N

V

A

N

Q

P

P

D

D

G

G

L

-

N

-

I

-

N

-

E

-

F

Y

S

F

G

P

A

A

T

R

K

N

P

P

E

E

L

P

L

N

R

E

K

E

N

N

-

L

-

K

-

E

-

F

-

M

-

E

-

I

V

V

L

K

A

A

E

L

E

G

A

A

D

D

I

F

G

G

I

V

A

A

L

Q

D
|
D

G

G

D
|
D

G

A

D
|
D

R
|
R

L

A

I

V

L

F

V

I

D

D

R

E

H

N

G

G

E

R

I

F

R

I

D

Q

G

G

D

D

D

K

I

T

L

F

Y

A

I

L

I

V

A

A

N

D

H

A

L

V

M

L

R

K

T

E

G

-

R

K

F

G

S

G

G

G

G

L

V

L

V

V

G

T

T

T

L

V

M

A

S

T

N

S

F

N

G

L

L

L

E

D

L

D

A

I

F

A

S

K

E

K

T

H

G

G

I

A

G

K

F

V

S

M

R

R

A

T

A

K

V

V

G

G

D

D

R

L

Y

I

V

V

N

A

E

R

K

A

L

L

M

Y

Q

E

H

N

G

N

W

G

V

T

L

I

G

G

G

G

E

E

P

E

S

N

G

G

H
x
G

I

V

V

I

C

F

R

P

S

E

I

H

T

V

T

L

T

G

G
x
R

D

D

G

G

I

A

I

M

A

T

A

V

L

A

Q

K

V

V

L

V

R

E

A

I

M

F

V

A

E

K

E

S

G

G

K

K

S

K

L

F

D

S

E

E

L

L

L

I

V

D

G

E

L

L

V

P

K

K

F

Y

P

Y

Q

Q

K

-

L

-

K

-

N

-

I

I

R

K

V

T

A

K

K

R

R

H

F

V

V

E

P

G

N

D

E

R

E

H

P

A

A

I

L

V

Q

N

K

K

A

V

I

A

A

E

V

M

A

A

N

R

E

E

R

R

-

G

-

Y

-

T

-

V

-

D

-

T

L

T

N

D

G

G

L

A

-

K

-

I

-

I

-

F

-

E

-

D

G

G

R

W

V

V

L

L

L

V

R

R

A

A

S

S

G

G

T

T

E

E

P

P

L

I

I

I

R

R

V

I

M

F

V

S

E

E

G

A

R

K

D

S

D

K

Q

E

T

K

V

A

D

Q

E

E

L

Y

V

L

D

N

Y

L

L

G

V

I

E

E

E

L

V

L

N

E

S

K

V

A

V

L

S

S

active site: R11 (= R11), S101 (= S99), H102 (= H100), K111 (= K109), D243 (= D238), D245 (= D240), D247 (= D242), R248 (= R243), G330 (≠ H326), R340 (≠ G336)
binding magnesium ion: S101 (= S99), D243 (= D238), D245 (= D240), D247 (= D242)

1pcjX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
26% identity, 97% coverage: 10:444/448 of query aligns to 14:453/458 of 1pcjX

query
sites
1pcjX

I

I

R
|
R

G

G

K

V

V

V

G

G

T

D

M

T

P

-

I

L

T

T

P

A

E

E

F

T

M

A

L

Y

K

W

L

I

G

G

W

R

A

A

A

I

G

G

-

S

Q

E

V

S

F

L

K

A

E

R

N

G

D

E

K

P

K

C

I

V

L

A

I

V

G

G

K

R

D

D

T

G

R

R

I

L

S

S

G

G

Y

P

M

E

F

L

E

V

S

K

A

Q

L

L

E

I

S

Q

G

G

I

L

V

V

A

D

A

C

G

G

A

C

D

Q

V

V

R

S

L

D

V

V

G

G

P

M

M

V

P

P

T

T

P

P

A

V

I

L

A

Y

Y

Y

L

A

T

A

R

N

T

V

F

L

R

E

A

G

S

K

A

S

G

G

I

V

V

M

I

L

S

T

A

G

S
|
S

H
|
H

N

N

P

P

Y

P

T

D

D

Y

N

N

G

G

I

F

K
|
K

F

I

F

V

S

V

A

A

E

G

G

E

G

T

K

L

I

A

S

N

D

E

E

Q

I

I

E

Q

E

A

R

L

I

R

E

E

Y

R

F

I

L

E

E

K

Q

N

P

D

M

L

V

-

V

-

V

-

E

-

S

A

S

S

L

G

I

V

G

G

R

S

A

V

K

E

R

Q

I

V

D

-

D

D

A

I

A

L

G

P

R

R

Y

Y

I

F

E

K

Y

Q

C

I

K

R

G

D

T

D

F

I

P

A

I

M

G

A

L

-

Q

-

L

-

S

K

G

P

L

M

K

K

I

V

V

V

V

V

D

D

C

C

A

G

D

N

G

G

A

V

T

A

Y

G

H

V

V

I

A

A

P

P

R

Q

V

L

F

I

S

E

E

A

L

L

G

G

A

C

E

S

V

V

I

I

S

P

I

L

G

Y

V

C

N

E

P

V

D

D

G

G

L

N

N

F

I

P

N

N

E

H

F

H

S

P

G

D

A

P

T

G

K

K

P

P

E

E

L

N

L

L

R

K

K

D

-

L

-

I

-

A

N

K

V

V

L

K

A

A

E

E

E

N

A

A

D

D

I

L

G

G

I

L

A

A

L

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

I

G

L

V

V

V

D

T

R

N

H

T

G

G

E

T

I

I

R

I

D

Y

G

P

D

D

D

R

I

L

L

L

Y

M

I

L

I

F

A

A

N

K

H

D

L

V

M

V

R

-

T

-

G

S

R

R

F

N

S

P

G

G

G

A

-

D

V

I

V

I

G

F

T

D

L

V

M

K

S

C

N

T

F

R

G

R

L

L

E

I

L

A

A

L

F

I

S

S

E

G

T

Y

G

G

I

G

G

R

F

P

S

V

R

M

A

W

A

K

V
x
T

G
|
G

D

H

R

S

Y

L

V

I

N

K

E

K

K

K

L

M

M

K

Q

E

H

T

G

G

W

A

V

L

L

L

G

A

G

G

E
|
E

P

M

S
|
S

G

G

H
|
H

I

V

V

F

C

F

R

K

S

E

-

R

I

W

T

F

T

G

T

F

G
x
D

D

D

G

G

I

I

I

Y

A

S

A

A

L

A

Q

R

V

L

L

L

R

E

A

I

M

L

V

S

E

Q

E

D

G

Q

K

R

S

D

L

S

D

E

E

H

L

V

L

F

V

S

G

A

L

-

V

-

K

-

F

F

P

P

Q

S

K

D

L

I

K

S

-

T

-

P

-

E

-

I

N

N

I

I

R

T

V

V

A

T

K

E

-

D

-

S

R

K

F

F

V

A

P

I

N

I

E

E

E

-

P

-

A

A

L

L

Q

Q

K

R

A

D

I

A

A

Q

V

W

A

G

N

E

E

G

R

N

L

I

N

T

G

T

L

L

-

D

-

G

-

V

-

R

-

V

-

D

-

Y

-

P

-

K

G

G

R

W

V

G

L

L

L

V

R
|
R

A

A

S
|
S

G
x
N

T
|
T

E

T

P

P

L

V

I

L

R

V

V

L

M

R

V

F

E

E

G

A

R

D

D

T

D

E

Q

E

T

E

V

L

D

E

E

R

L

I

V

K

D

T

Y

V

L

F

V

R

E

N

E

Q

V

L

N

K

S

A

V

V

V

D

S

S

A

S

active site: R15 (= R11), S103 (= S99), H104 (= H100), K113 (= K109), D237 (= D238), D239 (= D240), D241 (= D242), R242 (= R243), H324 (= H326), D335 (≠ G336)
binding 1-O-phosphono-alpha-D-mannopyranose: S103 (= S99), T301 (≠ V303), G302 (= G304), E320 (= E322), S322 (= S324), H324 (= H326), R416 (= R407), S418 (= S409), N419 (≠ G410), T420 (= T411)
binding zinc ion: S103 (= S99), D237 (= D238), D239 (= D240), D241 (= D242)

Sites not aligning to the query:

binding 1-O-phosphono-alpha-D-mannopyranose: 12

2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
27% identity, 97% coverage: 10:444/448 of query aligns to 11:450/455 of 2h5aX

query
sites
2h5aX

I

I

R

R

G

G

K

V

V

V

G

G

T

D

M

T

P

-

I

L

T

T

P

A

E

E

F

T

M

A

L

Y

K

W

L

I

G

G

W

R

A

A

A

I

G

G

-

S

Q

E

V

S

F

L

K

A

E

R

N

G

D

E

K

P

K

C

I

V

L

A

I

V

G

G

K

R

D

D

T

G

R

R

I

L

S

S

G

G

Y

P

M

E

F

L

E

V

S

K

A

Q

L

L

E

I

S

Q

G

G

I

L

V

V

A

D

A

C

G

G

A

C

D

Q

V

V

R

S

L

D

V

V

G

G

P

M

M

V

P

P

T

T

P

P

A

V

I

L

A

Y

Y

Y

L

A

T

A

R

N

T

V

F

L

R

E

A

G

S

K

A

S

G

G

I

V

V

M

I

L

S

T

A

G

S
|
S

H
|
H

N

N

P

P

Y

P

T

D

D

Y

N

N

G

G

I

F

K

K

F

I

F

V

S

V

A

A

E

-

G

G

G

E

K

T

I

L

S

A

D

N

E

E

I

Q

E

I

E

Q

R

A

I

L

E

R

Y

E

F

R

L

I

E

E

Q

K

P

N

M

D

V

L

V

-

V

-

E

-

S

A

S

S

L

G

I

V

G

G

R

S

A

V

K

E

R

Q

I

V

D

-

D

D

A

I

A

L

G

P

R

R

Y

Y

I

F

E

K

Y

Q

C

I

K

R

G

D

T

D

F

I

P

A

I

M

G

A

L

-

Q

-

L

-

S

K

G

P

L

M

K

K

I

V

V

V

V

V

D

D

C

C

A

G

D

N

G

G

A

V

T

A

Y

G

H

V

V

I

A

A

P

P

R

Q

V

L

F

I

S

E

E

A

L

L

G

G

A

C

E

S

V

V

I

I

S

P

I

L

G

Y

V

C

N

E

P

V

D

D

G

G

L

N

N

F

I

P

N

N

E

H

F

H

S

P

G

D

A

P

T

G

K

K

P

P

E

E

L

N

L

L

R

K

K

D

-

L

-

I

-

A

N

K

V

V

L

K

A

A

E

E

E

N

A

A

D

D

I

L

G

G

I

L

A

A

L

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

I

G

L

V

V

V

D

T

R

N

H

T

G

G

E

T

I

I

R

I

D

Y

G

P

D

D

D

R

I

L

L

L

Y

M

I

L

I

F

A

A

N

K

H

D

L

V

M

V

R

-

T

-

G

S

R

R

F

N

S

P

G

G

G

A

-

D

V

I

V

I

G

F

T

D

L

V

M

K

S

C

N

T

F

R

G

R

L

L

E

I

L

A

A

L

F

I

S

S

E

G

T

Y

G

G

I

G

G

R

F

P

S

V

R

M

A

W

A

K

V
x
T

G
|
G

D
x
H

R

S

Y

L

V

I

N

K

E

K

K

K

L

M

M

K

Q

E

H

T

G

G

W

A

V

L

L

L

G

A

G

G

E
|
E

P

M

S
|
S

G

G

H
|
H

I

V

V

F

C

F

R

K

S

E

-

R

I

W

T

F

T

G

T

F

G
x
D

D

D

G

G

I

I

I

Y

A

S

A

A

L

A

Q

R

V

L

L

L

R

E

A

I

M

L

V

S

E

Q

E

D

G

Q

K

R

S

D

L

S

D

E

E

H

L

V

L

F

V

S

G

A

L

-

V

-

K

-

F

F

P

P

Q

S

K

D

L

I

K

S

-

T

-

P

-

E

-

I

N

N

I

I

R

T

V

V

A

T

K

E

-

D

-

S

R

K

F

F

V

A

P

I

N

I

E

E

E

-

P

-

A

A

L

L

Q

Q

K

R

A

D

I

A

A

Q

V

W

A

G

N

E

E

G

R

N

L

I

N

T

G

T

L

L

-

D

-

G

-

V

-

R

-

V

-

D

-

Y

-

P

-

K

G

G

R

W

V

G

L

L

L

V

R
|
R

A

A

S
|
S

G
x
N

T
|
T

E

T

P

P

L

V

I

L

R

V

V

L

M

R

V

F

E

E

G

A

R

D

D

T

D

E

Q

E

T

E

V

L

D

E

E

R

L

I

V

K

D

T

Y

V

L

F

V

R

E

N

E

Q

V

L

N

K

S

A

V

V

V

D

S

S

A

S

active site: H101 (= H100), D234 (= D238), D236 (= D240), D238 (= D242), R239 (= R243), D332 (≠ G336)
binding 1-O-phosphono-alpha-D-xylopyranose: T298 (≠ V303), G299 (= G304), H300 (≠ D305), E317 (= E322), S319 (= S324), H321 (= H326), R413 (= R407), S415 (= S409), N416 (≠ G410), T417 (= T411)
binding zinc ion: S100 (= S99), D234 (= D238), D236 (= D240), D238 (= D242)

Sites not aligning to the query:

binding 1-O-phosphono-alpha-D-xylopyranose: 9

2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
27% identity, 97% coverage: 10:444/448 of query aligns to 11:450/455 of 2h4lX

query
sites
2h4lX

I

I

R
|
R

G

G

K

V

V

V

G

G

T

D

M

T

P

-

I

L

T

T

P

A

E

E

F

T

M

A

L

Y

K

W

L

I

G

G

W

R

A

A

A

I

G

G

-

S

Q

E

V

S

F

L

K

A

E

R

N

G

D

E

K

P

K

C

I

V

L

A

I

V

G

G

K

R

D

D

T

G

R

R

I

L

S

S

G

G

Y

P

M

E

F

L

E

V

S

K

A

Q

L

L

E

I

S

Q

G

G

I

L

V

V

A

D

A

C

G

G

A

C

D

Q

V

V

R

S

L

D

V

V

G

G

P

M

M

V

P

P

T

T

P

P

A

V

I

L

A

Y

Y

Y

L

A

T

A

R

N

T

V

F

L

R

E

A

G

S

K

A

S

G

G

I

V

V

M

I

L

S

T

A

G

S
|
S

H
|
H

N

N

P

P

Y

P

T

D

D

Y

N

N

G

G

I

F

K

K

F

I

F

V

S

V

A

A

E

-

G

G

G

E

K

T

I

L

S

A

D

N

E

E

I

Q

E

I

E

Q

R

A

I

L

E

R

Y

E

F

R

L

I

E

E

Q

K

P

N

M

D

V

L

V

-

V

-

E

-

S

A

S

S

L

G

I

V

G

G

R

S

A

V

K

E

R

Q

I

V

D

-

D

D

A

I

A

L

G

P

R

R

Y

Y

I

F

E

K

Y

Q

C

I

K

R

G

D

T

D

F

I

P

A

I

M

G

A

L

-

Q

-

L

-

S

K

G

P

L

M

K

K

I

V

V

V

V

V

D

D

C

C

A

G

D

N

G

G

A

V

T

A

Y

G

H

V

V

I

A

A

P

P

R

Q

V

L

F

I

S

E

E

A

L

L

G

G

A

C

E

S

V

V

I

I

S

P

I

L

G

Y

V

C

N

E

P

V

D

D

G

G

L

N

N

F

I

P

N

N

E

H

F

H

S

P

G

D

A

P

T

G

K

K

P

P

E

E

L

N

L

L

R

K

K

D

-

L

-

I

-

A

N

K

V

V

L

K

A

A

E

E

E

N

A

A

D

D

I

L

G

G

I

L

A

A

L

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

I

G

L

V

V

V

D

T

R

N

H

T

G

G

E

T

I

I

R

I

D

Y

G

P

D

D

D

R

I

L

L

L

Y

M

I

L

I

F

A

A

N

K

H

D

L

V

M

V

R

-

T

-

G

S

R

R

F

N

S

P

G

G

G

A

-

D

V

I

V

I

G

F

T

D

L

V

M

K

S

C

N

T

F

R

G

R

L

L

E

I

L

A

A

L

F

I

S

S

E

G

T

Y

G

G

I

G

G

R

F

P

S

V

R

M

A

W

A

K

V
x
T

G

G

D

H

R

S

Y

L

V

I

N

K

E

K

K

K

L

M

M

K

Q

E

H

T

G

G

W

A

V

L

L

L

G

A

G

G

E
|
E

P

M

S

S

G

G

H

H

I

V

V

F

C

F

R

K

S

E

-

R

I

W

T

F

T

G

T

F

G
x
D

D

D

G

G

I

I

I

Y

A

S

A

A

L

A

Q

R

V

L

L

L

R

E

A

I

M

L

V

S

E

Q

E

D

G

Q

K

R

S

D

L

S

D

E

E

H

L

V

L

F

V

S

G

A

L

-

V

-

K

-

F

F

P

P

Q

S

K

D

L

I

K

S

-

T

-

P

-

E

-

I

N

N

I

I

R

T

V

V

A

T

K

E

-

D

-

S

R

K

F

F

V

A

P

I

N

I

E

E

E

-

P

-

A

A

L

L

Q

Q

K

R

A

D

I

A

A

Q

V

W

A

G

N

E

E

G

R

N

L

I

N

T

G

T

L

L

-

D

-

G

-

V

-

R

-

V

-

D

-

Y

-

P

-

K

G

G

R

W

V

G

L

L

L

V

R
|
R

A

A

S
|
S

G
x
N

T
|
T

E

T

P

P

L

V

I

L

R

V

V

L

M

R

V

F

E

E

G

A

R

D

D

T

D

E

Q

E

T

E

V

L

D

E

E

R

L

I

V

K

D

T

Y

V

L

F

V

R

E

N

E

Q

V

L

N

K

S

A

V

V

V

D

S

S

A

S

active site: H101 (= H100), D234 (= D238), D236 (= D240), D238 (= D242), R239 (= R243), D332 (≠ G336)
binding 1-O-phosphono-alpha-D-ribofuranose: R12 (= R11), S100 (= S99), T298 (≠ V303), E317 (= E322), R413 (= R407), S415 (= S409), N416 (≠ G410), T417 (= T411)
binding zinc ion: S100 (= S99), D234 (= D238), D236 (= D240), D238 (= D242)

Sites not aligning to the query:

binding 1-O-phosphono-alpha-D-ribofuranose: 9

2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
27% identity, 97% coverage: 10:444/448 of query aligns to 11:450/455 of 2fkfA

query
sites
2fkfA

I

I

R
|
R

G

G

K

V

V

V

G

G

T

D

M

T

P

-

I

L

T

T

P

A

E

E

F

T

M

A

L

Y

K

W

L

I

G

G

W

R

A

A

A

I

G

G

-

S

Q

E

V

S

F

L

K

A

E

R

N

G

D

E

K

P

K

C

I

V

L

A

I

V

G

G

K

R

D

D

T

G

R

R

I

L

S

S

G

G

Y

P

M

E

F

L

E

V

S

K

A

Q

L

L

E

I

S

Q

G

G

I

L

V

V

A

D

A

C

G

G

A

C

D

Q

V

V

R

S

L

D

V

V

G

G

P

M

M

V

P

P

T

T

P

P

A

V

I

L

A

Y

Y

Y

L

A

T

A

R

N

T

V

F

L

R

E

A

G

S

K

A

S

G

G

I

V

V

M

I

L

S

T

A

G

S
|
S

H
|
H

N

N

P

P

Y

P

T

D

D

Y

N

N

G

G

I

F

K
|
K

F

I

F

V

S

V

A

A

E

-

G

G

G

E

K

T

I

L

S

A

D

N

E

E

I

Q

E

I

E

Q

R

A

I

L

E

R

Y

E

F

R

L

I

E

E

Q

K

P

N

M

D

V

L

V

-

V

-

E

-

S

A

S

S

L

G

I

V

G

G

R

S

A

V

K

E

R

Q

I

V

D

-

D

D

A

I

A

L

G

P

R

R

Y

Y

I

F

E

K

Y

Q

C

I

K

R

G

D

T

D

F

I

P

A

I

M

G

A

L

-

Q

-

L

-

S

K

G

P

L

M

K

K

I

V

V

V

V

V

D

D

C

C

A

G

D

N

G

G

A

V

T

A

Y

G

H

V

V

I

A

A

P

P

R

Q

V

L

F

I

S

E

E

A

L

L

G

G

A

C

E

S

V

V

I

I

S

P

I

L

G

Y

V

C

N

E

P

V

D

D

G

G

L

N

N

F

I

P

N

N

E

H

F

H

S

P

G

D

A

P

T

G

K

K

P

P

E

E

L

N

L

L

R

K

K

D

-

L

-

I

-

A

N

K

V

V

L

K

A

A

E

E

E

N

A

A

D

D

I

L

G

G

I

L

A

A

L

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

I

G

L

V

V

V

D

T

R

N

H

T

G

G

E

T

I

I

R

I

D

Y

G

P

D

D

D

R

I

L

L

L

Y

M

I

L

I

F

A

A

N

K

H

D

L

V

M

V

R

-

T

-

G

S

R

R

F

N

S

P

G

G

G

A

-

D

V

I

V

I

G

F

T

D

L

V

M

K

S

C

N

T

F

R

G

R

L

L

E

I

L

A

A

L

F

I

S

S

E

G

T

Y

G

G

I

G

G

R

F

P

S

V

R

M

A

W

A

K

V

T

G

G

D

H

R

S

Y

L

V

I

N

K

E

K

K

K

L

M

M

K

Q

E

H

T

G

G

W

A

V

L

L

L

G

A

G

G

E

E

P

M

S
|
S

G

G

H
|
H

I

V

V

F

C

F

R

K

S

E

-

R

I

W

T

F

T

G

T

F

G
x
D

D

D

G

G

I

I

I

Y

A

S

A

A

L

A

Q

R

V

L

L

L

R

E

A

I

M

L

V

S

E

Q

E

D

G

Q

K

R

S

D

L

S

D

E

E

H

L

V

L

F

V

S

G

A

L

-

V

-

K

-

F

F

P

P

Q

S

K

D

L

I

K

S

-

T

-

P

-

E

-

I

N

N

I

I

R

T

V

V

A

T

K

E

-

D

-

S

R

K

F

F

V

A

P

I

N

I

E

E

E

-

P

-

A

A

L

L

Q

Q

K

R

A

D

I

A

A

Q

V

W

A

G

N

E

E

G

R

N

L

I

N

T

G

T

L

L

-

D

-

G

-

V

-

R

-

V

-

D

-

Y

-

P

-

K

G

G

R

W

V

G

L

L

L

V

R
|
R

A

A

S
|
S

G
x
N

T
|
T

E

T

P

P

L

V

I

L

R

V

V

L

M

R

V

F

E

E

G

A

R

D

D

T

D

E

Q

E

T

E

V

L

D

E

E

R

L

I

V

K

D

T

Y

V

L

F

V

R

E

N

E

Q

V

L

N

K

S

A

V

V

V

D

S

S

A

S

active site: R12 (= R11), S100 (= S99), H101 (= H100), K110 (= K109), D234 (= D238), D236 (= D240), D238 (= D242), R239 (= R243), H321 (= H326), D332 (≠ G336)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: H101 (= H100), S319 (= S324), R413 (= R407), S415 (= S409), N416 (≠ G410), T417 (= T411)
binding zinc ion: S100 (= S99), D234 (= D238), D236 (= D240), D238 (= D242)

Sites not aligning to the query:

binding 1,6-di-O-phosphono-alpha-D-glucopyranose: 7

1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
27% identity, 97% coverage: 10:444/448 of query aligns to 11:450/455 of 1pcmX

query
sites
1pcmX

I

I

R
|
R

G

G

K

V

V

V

G

G

T

D

M

T

P

-

I

L

T

T

P

A

E

E

F

T

M

A

L

Y

K

W

L

I

G

G

W

R

A

A

A

I

G

G

-

S

Q

E

V

S

F

L

K

A

E

R

N

G

D

E

K

P

K

C

I

V

L

A

I

V

G

G

K

R

D

D

T

G

R

R

I

L

S

S

G

G

Y

P

M

E

F

L

E

V

S

K

A

Q

L

L

E

I

S

Q

G

G

I

L

V

V

A

D

A

C

G

G

A

C

D

Q

V

V

R

S

L

D

V

V

G

G

P

M

M

V

P

P

T

T

P

P

A

V

I

L

A

Y

Y

Y

L

A

T

A

R

N

T

V

F

L

R

E

A

G

S

K

A

S

G

G

I

V

V

M

I

L

S

T

A

G

S
|
S

H
|
H

N

N

P

P

Y

P

T

D

D

Y

N

N

G

G

I

F

K
|
K

F

I

F

V

S

V

A

A

E

-

G

G

G

E

K

T

I

L

S

A

D

N

E

E

I

Q

E

I

E

Q

R

A

I

L

E

R

Y

E

F

R

L

I

E

E

Q

K

P

N

M

D

V

L

V

-

V

-

E

-

S

A

S

S

L

G

I

V

G

G

R

S

A

V

K

E

R

Q

I

V

D

-

D

D

A

I

A

L

G

P

R

R

Y

Y

I

F

E

K

Y

Q

C

I

K

R

G

D

T

D

F

I

P

A

I

M

G

A

L

-

Q

-

L

-

S

K

G

P

L

M

K

K

I

V

V

V

V

V

D

D

C

C

A

G

D

N

G

G

A

V

T

A

Y

G

H

V

V

I

A

A

P

P

R

Q

V

L

F

I

S

E

E

A

L

L

G

G

A

C

E

S

V

V

I

I

S

P

I

L

G

Y

V

C

N

E

P

V

D

D

G

G

L

N

N

F

I

P

N

N

E

H

F

H

S

P

G

D

A

P

T

G

K

K

P

P

E

E

L

N

L

L

R

K

K

D

-

L

-

I

-

A

N

K

V

V

L

K

A

A

E

E

E

N

A

A

D

D

I

L

G

G

I

L

A

A

L

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

L

V

I

G

L

V

V

V

D

T

R

N

H

T

G

G

E

T

I

I

R

I

D

Y

G

P

D

D

D

R

I

L

L

L

Y

M

I

L

I

F

A

A

N

K

H

D

L

V

M

V

R

-

T

-

G

S

R

R

F

N

S

P

G

G

G

A

-

D

V

I

V

I

G

F

T

D

L

V

M

K

S

C

N

T

F

R

G

R

L

L

E

I

L

A

A

L

F

I

S

S

E

G

T

Y

G

G

I

G

G

R

F

P

S

V

R

M

A

W

A

K

V
x
T

G
|
G

D
x
H

R

S

Y

L

V

I

N

K

E

K

K

K

L

M

M

K

Q

E

H

T

G

G

W

A

V

L

L

L

G

A

G

G

E
|
E

P

M

S
|
S

G

G

H
|
H

I

V

V

F

C

F

R

K

S

E

-

R

I

W

T

F

T

G

T

F

G
x
D

D

D

G

G

I

I

I

Y

A

S

A

A

L

A

Q

R

V

L

L

L

R

E

A

I

M

L

V

S

E

Q

E

D

G

Q

K

R

S

D

L

S

D

E

E

H

L

V

L

F

V

S

G

A

L

-

V

-

K

-

F

F

P

P

Q

S

K

D

L

I

K

S

-

T

-

P

-

E

-

I

N

N

I

I

R

T

V

V

A

T

K

E

-

D

-

S

R

K

F

F

V

A

P

I

N

I

E

E

E

-

P

-

A

A

L

L

Q

Q

K

R

A

D

I

A

A

Q

V

W

A

G

N

E

E

G

R

N

L

I

N

T

G

T

L

L

-

D

-

G

-

V

-

R

-

V

-

D

-

Y

-

P

-

K

G

G

R

W

V

G

L

L

L

V

R
|
R

A

A

S
|
S

G

N

T

T

E

T

P

P

L

V

I

L

R

V

V

L

M

R

V

F

E

E

G

A

R

D

D

T

D

E

Q

E

T

E

V

L

D

E

E

R

L

I

V

K

D

T

Y

V

L

F

V

R

E

N

E

Q

V

L

N

K

S

A

V

V

V

D

S

S

A

S

active site: R12 (= R11), S100 (= S99), H101 (= H100), K110 (= K109), D234 (= D238), D236 (= D240), D238 (= D242), R239 (= R243), H321 (= H326), D332 (≠ G336)
binding 6-O-phosphono-alpha-D-mannopyranose: S100 (= S99), T298 (≠ V303), G299 (= G304), H300 (≠ D305), E317 (= E322), S319 (= S324), H321 (= H326), R413 (= R407), S415 (= S409)
binding zinc ion: S100 (= S99), D234 (= D238), D236 (= D240), D238 (= D242)

Sites not aligning to the query:

binding 6-O-phosphono-alpha-D-mannopyranose: 9

1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
27% identity, 97% coverage: 10:444/448 of query aligns to 11:450/455 of 1p5gX

query
sites
1p5gX

I

I

R
|
R

G

G

K

V

V

V

G

G

T

D

M

T

P

-

I

L

T

T

P

A

E

E

F

T

M

A

L

Y

K

W

L

I

G

G

W

R

A

A

A

I

G

G

-

S

Q

E

V

S

F

L

K

A

E

R

N

G

D

E

K

P

K

C

I

V

L

A

I

V

G

G

K

R

D

D

T

G

R

R

I

L

S

S

G

G

Y