SitesBLAST

E205 (= E230) mutation to A: Has no effect on the growth rate.
E208 (= E233) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D238) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
24% identity, 73% coverage: 91:334/336 of query aligns to 259:514/514 of P02916

query
sites
P02916

V

T

G

G

L

W

S

K

N

N

Y

F

V

T

W

R

L

V

F

F

G

T

S

D

D

E

E

G

F

I

F

Q

E

K

S

P

F

F

R

L

N

A

N

I

M

F

L

V

W

W

L

T

I

V

V

V

V

F

P

S

T

L

A

I

A

T

T

V

F

F

F

L

-

T

-

V

-

A

-

V

G

G

L

M

I

V

I

L

A

A

T

C

M

L

T

V

-

Q

-

W

D

E

R

A

I

L

W

R

W

G

G

K

N

A

I

V

A

Y

K

R

S

V

L

L

I

L

F

I

M

L

P

P

M

Y

A

A

I

V

S

P

F

S

I

F

G

I

A

S

S

I

V
x
L

I

I

W

F

K

K

F

G

I

L

Y

F

D

N

F

-

R

Q

S

S

G

F

G

G

D

E

A

I

Q

N

I

M

G

-

L

M

L

L

N

S

A

A

V

L

V

-

F

-

F

F

G

G

G

V

E

K

P

P

Q

A

N

-

W

W

I

F

T

S

I

D

P

P

F

T

W

T

N

A

N

R

F

T

F

M

L
|
L

M

I

I

I

I

V

L
x
N

V

T

W

W

I

L

Q
x
G

T

Y

G

P

F

Y

A

M

M

M

V

I

I

L

L

C

S

M

A

G

A

L

L

L

R

K

G

A

I

I

P

P

D

D

E

D

T

L

I

Y

E
|
E

A

A

A
x
S

V

A

L

M

D

D

G
|
G

A

A

N

G

G

P

L

F

Q

Q

I

N

F

F

K

K

I

I

M

T

V

L

P
|
P

Q

L

I

L

W

I

G

K

T

P

I

L

A

T

V

P

V

L

W

M

T

I

T

A

I

S

T

F

I

A

L

F

V

N

L

F

K

N

V

N

F

F

D

V

I

L

V

I

L

Q

A

L

M

L

T

T

N

N

G

G

Q

G

W

P

D

D

-

R

-

L

-

G

-

T

-

P

-

A

-

G

-

Y

T

T

Q

D

V

L

L

L

A

V

N

N

Q

Y

M

T

F

Y

D

R

W

I

M

A

F

F

R

E

G

G

-

G

-

Q

D

D

F

F

G

G

R

L

G

A

A

A

V

A

V

I

A

A

I

T

V

L

I

I

M

F

V

L

L

L

V

V

I

G

P

A

I

L

M

A

V

I

W

V

N

N

I

L

K

K

R

A

A

T

N

R

A

M

E

K

M

F

E

D

L334 (≠ V160) mutation to W: Ability to transport lactose in a saturable manner.
L372 (= L204) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ L208) mutation N->K,H: No growth on maltose.
G380 (≠ Q212) mutation G->C,S: No growth on maltose.
E401 (= E233) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A235) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G239) mutation G->A,P: No effect.
P420 (= P252) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
25% identity, 70% coverage: 91:325/336 of query aligns to 244:490/490 of 4ki0F

query
sites
4ki0F

V

T

G

G

L

W

S

K

N

N

Y

F

V

T

W

R

L

V

F

F

G

T

S

D

D

E

E

G

F

I

F

Q

E

K

S

P

F

F

R

L

N

A

N

I

M

F

L

V

W

W

L

T

I

V

V

V

V

F

P

S

T

L

A

I

A

T

T

V

F

F

F

L

-

T

-

V

-

A

-

V

G

G

L

M

I

V

I

L

A

A

T

C

M

L

T

V

-

Q

-

W

D

E

R

A

I

L

W

R

W

G

G

K

N

A

I

V

A

Y

K

R

S

V

L

L

I

L

F

I

M

L

P

P

M
x
Y

A

A

I

V

S

P

F

S

I

F

G

I

A

S

S

I

V

L

I

I

W

F

K

K

F

G

I

L

Y

F

D

N

F

-

R

Q

S

S

G

F

G

G

D

E

A

I

Q

N

I

M

G

-

L

M

L

L

N

S

A

A

V

L

V

-

F

-

F

F

G

G

G

V

E

K

P

P

Q

A

N

-

W

W

I

F

T

S

I

D

P

P

F

T

W

T

N

A

N

R

F

T

F

M

L

L

M

I

I

I

I

V

L
x
N

V

T

W

W

I

L

Q
x
G

T

Y

G

P

F
x
Y

A

M

M

M

V

I

I

L

L

C

S

M

A

G

A

L

L

L

R

K

G

A

I

I

P

P

D

D

E

D

T

L

I

Y

E

E

A

A

A

S

V

A

L

M

D

D

G

G

A

A

N

G

G

P

L

F

Q

Q

I

N

F

F

K

K

I

I

M

T

V

L

P

P

Q

L

I

L

W

I

G

K

T

P

I

L

A

T

V

P

V

L

W

M

T

I

T

A

I
x
S

T

F

I

A

L

F

V
x
N

L

F

K

N

V

N

F

F

D

V

I

L

V

I

L

Q

A

L

M

L

T

T

N

N

G

G

Q

G

W

P

D

D

-

R

-

L

-

G

-

T

-

P

-

A

-

G

-

Y

T

T

Q

D

V

L

L

L

A

V

N

N

Q

Y

M

T

F

Y

D

R

W

I

M

A

F

F

R

E

G

G

-

G

-

Q

D

D

F

F

G

G

R

L

G

A

A

A

V

A

V

I

A

A

I

T

V

L

I

I

M

F

V

L

L

L

V

V

I

G

P

A

I

L

M

A

V

I

W

V

N

N

binding alpha-D-glucopyranose: Y310 (≠ M151), N361 (≠ L208), G365 (≠ Q212), Y368 (≠ F215), S418 (≠ I265), N422 (≠ V269)

7cagA Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
26% identity, 83% coverage: 56:335/336 of query aligns to 12:285/285 of 7cagA

query
sites
7cagA

W

W

L

L

F

-

L

I

G

A

P

P

A

A

V

V

L

L

L

M

T

L

F

A

Y

V

L

T

F

A

Y

Y

P

P

V

I

I

G

D

Y

S

A

I

V

-

W

-

L

-

S

-

L

-

Q

R

R

L

Y

S

N

F

L

F

A

D

E

R

P

S

H

G

D

E

T

E

E

F

F

V

I

G

G

L

L

S

A

N

N

Y

Y

V

V

W

T

L

V

F

L

G

T

S

D

D

G

E

Y

F

W

E

T

S

A

F

F

R

A

N

V

N

T

M

-

L

L

W

G

L

I

I

T

V

V

P

S

T

V

A

A

A

I

T

E

F

F

-

A

F

L

G

G

L

L

I

A

I

L

A

A

T

L

M

V

T

M

D

H

R

R

I

T

W

I

W

F

G

G

N

K

I

G

A

A

-

V

K

R

S

T

L

A

I

I

F

L

M

I

P

P

M

Y

A

G

I

I

S

V

F

T

I

V

G

A

A

A

S

S

V

Y

I

S

W

W

K

Y

F

Y

I

A

Y

W

D

T

F

P

R

G

S

T

G

G

G

Y

D

L

A

A

Q

N

I

L

G

-

L

-

N

-

A

-

V

-

V

L

F

P

F

E

G

G

G

S

E

A

P

P

Q

-

N

-

W

-

I

L

T

T

I

D

P

Q

F

L

W

P

N

S

N

L

F

A

F

I

L

V

M

V

I

L

I

A

L
x
E

V

V

W

W

I
x
K

Q

T

T

T

G

P

F

F

A

M

M

A

V

L

I

L

L

L

S

L

A

A

A

G

L

L

R

A

G

L

I

V

P

P

D

Q

E

D

T

L

I

L

E

N

A

A

V

Q

L

V

D

D

G

G

A

A

N

G

G

P

L

W

Q

K

I

R

F

L

F

T

K

K

I

V

M

I

V

L

P

P

Q

M

I

I

W

K

G

P

T

A

I

I

A

L

V

V

V

A

W

L

T

L

T

F

I
x
R

T

T

I

L

L
x
D

V

A

L

F

K

R

V

I

F

F

D

D

I

N

V

I

L

Y

A

I

M

L

T

T

N

G

G

G

Q

S

W

N

D

D

T

T

Q

G

V

S

L

V

A

S

N

I

Q

L

M

G

F

Y

D

D

W

N

M

L

F

F

R

K

G

-

G

A

G

F

D

N

F

V

G

G

R

L

G

G

A

S

V

A

V

I

A

S

I

V

V

L

I

I

M

F

V

L

L

S

V

V

I

A

P

I

I

I

M

A

V

F

W

I

N

Y

I

I

K

K

R

I

A

F

N

G

A

A

E

A

M

A

E

P

G

G

binding alpha-D-glucopyranose: E159 (≠ L208), K162 (≠ I211), R216 (≠ I265), D219 (≠ L268)

Query Sequence

>WP_111605924.1 NCBI__GCF_003259225.1:WP_111605924.1
MAQLASALVTMILGVLGCAVYFYGSNILLDKIFPTKNRPAQQVASNLRIANSIRPWLFLG
PAVLLLTFYLFYPVIDSIRLSFFDRSGEEFVGLSNYVWLFGSDEFFESFRNNMLWLIVVP
TAATFFGLIIATMTDRIWWGNIAKSLIFMPMAISFIGASVIWKFIYDFRSGGDAQIGLLN
AVVVFFGGEPQNWITIPFWNNFFLMIILVWIQTGFAMVILSAALRGIPDETIEAAVLDGA
NGLQIFFKIMVPQIWGTIAVVWTTITILVLKVFDIVLAMTNGQWDTQVLANQMFDWMFRG
GGDFGRGAVVAIVIMVLVIPIMVWNIKRANAEMEGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory