SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_111607157.1 NCBI__GCF_003259225.1:WP_111607157.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

3nojA The structure of hmg/cha aldolase from the protocatechuate degradation pathway of pseudomonas putida (see paper)
58% identity, 100% coverage: 2:225/225 of query aligns to 6:229/235 of 3nojA

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A5W059 4-hydroxy-4-methyl-2-oxoglutarate aldolase/4-carboxy-4-hydroxy-2-oxoadipate aldolase; HMG/CHA aldolase; Oxaloacetate decarboxylase; OAA decarboxylase; EC 4.1.3.17; EC 4.1.1.112 from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) (see paper)
58% identity, 100% coverage: 2:225/225 of query aligns to 7:230/238 of A5W059

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1nxjA Structure of rv3853 from mycobacterium tuberculosis (see paper)
31% identity, 49% coverage: 65:175/225 of query aligns to 46:156/156 of 1nxjA

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1nxjA
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Sites not aligning to the query:

Query Sequence

>WP_111607157.1 NCBI__GCF_003259225.1:WP_111607157.1
MRNNVVVQNIERADQAVIDGLAECGVATIHESQGRVGLLAHYMRPIQMDKTIAGSAITIS
GAAGDNWMMHVAIEQCKAGDVMVFAPTSPSNHGFFGDLLATSAQSRGVVGLVIDGGVRDT
RDLRQMNFPVWSKAVFAEGTIKETVGSVNVPIVCADALVNPGDVIVADDDGVVVVRRENA
AEVLELARKRMANEEAKRVRMANGELGLDIYEMRPRLAEKGLKYV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory