SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_144019464.1 NCBI__GCF_900167125.1:WP_144019464.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
34% identity, 85% coverage: 6:138/157 of query aligns to 5:136/168 of 6addA

query
sites
6addA

V

V

V

V

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K

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D

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binding coenzyme a: Y24 (≠ E27), I28 (≠ L31), V75 (≠ L77), A76 (≠ V78), V77 (= V79), T82 (≠ R84), G83 (= G85), H84 (≠ R86), G85 (= G87), G87 (= G89), H88 (≠ R90), T110 (= T112), E112 (≠ T114), F115 (= F117)
binding n~2~-acetyl-l-glutamine: K27 (≠ G30), I28 (≠ L31), L30 (= L33), T74 (≠ S76), L109 (= L111)

Sites not aligning to the query:

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
34% identity, 85% coverage: 6:138/157 of query aligns to 5:136/168 of 5yo2A

query
sites
5yo2A

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A

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binding acetyl coenzyme *a: Y24 (≠ E27), I28 (≠ L31), I72 (≠ V74), R73 (= R75), T74 (≠ S76), V75 (≠ L77), V77 (= V79), T82 (≠ R84), G83 (= G85), H84 (≠ R86), G85 (= G87), G87 (= G89), H88 (≠ R90), T110 (= T112), E112 (≠ T114), F115 (= F117), F116 (= F118)
binding arginine: G26 (≠ E29), L29 (≠ V32), L30 (= L33), R73 (= R75), L109 (= L111)

Sites not aligning to the query:

binding arginine: 147

5ygeA Arga complexed with acecoa and glutamate (see paper)
34% identity, 85% coverage: 6:138/157 of query aligns to 6:137/169 of 5ygeA

query
sites
5ygeA

V

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binding acetyl coenzyme *a: Y25 (≠ E27), I73 (≠ V74), V76 (≠ L77), A77 (≠ V78), V78 (= V79), T83 (≠ R84), G84 (= G85), H85 (≠ R86), G86 (= G87), G88 (= G89), H89 (≠ R90), T111 (= T112), E113 (≠ T114), F117 (= F118)
binding glutamic acid: K33 (≠ R35), E44 (≠ D46), H64 (≠ S65), R74 (= R75)

Sites not aligning to the query:

binding acetyl coenzyme *a: 1, 2

4i49A Structure of ngnags bound with bisubstrate analog coa-NAG (see paper)
31% identity, 84% coverage: 7:138/157 of query aligns to 277:407/424 of 4i49A

query
sites
4i49A

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binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): I300 (≠ L31), L301 (≠ V32), L302 (= L33), R304 (= R35), A343 (≠ R75), C344 (≠ S76), L345 (= L77), A346 (≠ V78), V347 (= V79), Q352 (≠ R84), D353 (≠ G85), G355 (= G87), G357 (= G89), E358 (≠ R90), L379 (= L111), S380 (vs. gap), T383 (= T114), W386 (≠ F117), F387 (= F118)

Sites not aligning to the query:

binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): 413, 415

3d2mA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-glutamate (see paper)
31% identity, 84% coverage: 7:138/157 of query aligns to 277:407/424 of 3d2mA

query
sites
3d2mA

V

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binding coenzyme a: L345 (= L77), V347 (= V79), Q352 (≠ R84), D353 (≠ G85), G355 (= G87), G357 (= G89), E358 (≠ R90), S380 (vs. gap), T383 (= T114), W386 (≠ F117)
binding glutamic acid: L302 (= L33), R304 (= R35), A343 (≠ R75), C344 (≠ S76), L379 (= L111)

Sites not aligning to the query:

active site: 26
binding glutamic acid: 413, 415

3b8gA Crysta structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and n-acetyl-glutamate (see paper)
31% identity, 84% coverage: 7:138/157 of query aligns to 277:407/424 of 3b8gA

query
sites
3b8gA

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binding coenzyme a: I300 (≠ L31), L345 (= L77), V347 (= V79), Q352 (≠ R84), D353 (≠ G85), G355 (= G87), Y356 (≠ L88), G357 (= G89), E358 (≠ R90), S380 (vs. gap), T383 (= T114), E385 (= E116), W386 (≠ F117)
binding n-acetyl-l-glutamate: L302 (= L33), R304 (= R35), A343 (≠ R75), C344 (≠ S76), L379 (= L111)

Sites not aligning to the query:

active site: 26
binding n-acetyl-l-glutamate: 413, 415

2r8vA Native structure of n-acetylglutamate synthase from neisseria gonorrhoeae (see paper)
31% identity, 84% coverage: 7:138/157 of query aligns to 277:407/424 of 2r8vA

query
sites
2r8vA

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binding acetyl coenzyme *a: I300 (≠ L31), L301 (≠ V32), L345 (= L77), V347 (= V79), Q352 (≠ R84), D353 (≠ G85), G355 (= G87), G357 (= G89), E358 (≠ R90), T383 (= T114), E385 (= E116), W386 (≠ F117), F387 (= F118)

Sites not aligning to the query:

active site: 26

3d2pA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-arginine (see paper)
29% identity, 87% coverage: 7:142/157 of query aligns to 285:415/424 of 3d2pA

query
sites
3d2pA

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P

A

H

M

I

H

A

A

A

M

L

L

I

L

-

D

-

N

R

P

P

E
x
L

E

-

E

-

G

-

L

I

V

L

L

L

P

H

R

R

S

S

F

R

N

E

K

Y

L

L

Y

E

T

N

L

H

L

I

R

S

D

E

F

F

F

S

V

I

V

L

V

-

E

-

K

E

N

H

S

D

G

G

E

N

L

L

A

Y

G

G

C

C

C

A

A

A

L

L

S

K

I

T

C

F

W

A

E

E

-

A

E

D

L

C

A

G

E

E

V

I

R

A

S

C

L
|
L

V

A

V
|
V

R

S

N

P

T

Q

F

A

R
x
Q

G
x
D

R

G

G
|
G

L

Y

G
|
G

R
x
E

R

R

L

L

V

L

D

A

A

H

C

I

L

I

S

D

E

K

A

A

V

R

T

G

L

I

E

G

L

I

Y

S

K

R

V

L

F

F

V

A

L

L

-

S

T

T

N

N

T

T

P

G

E
|
E

F
x
W

F

F

A

A

H

E

L

R

G

G

F

F

R

Q

E

T

V

A

P

S

K

E

D

D

V

E

L

L

P

P

Q

E

K

T

V

R

W

R

S

K

D

D

C

Y

L

R

N

N

C

S

binding coenzyme a: L303 (≠ E27), L349 (= L77), V351 (= V79), Q356 (≠ R84), D357 (≠ G85), G359 (= G87), G361 (= G89), E362 (≠ R90), E389 (= E116), W390 (≠ F117)

Sites not aligning to the query:

active site: 26
binding arginine: 13, 197, 216, 253, 266, 267, 269, 270, 271, 274, 276

P0A6C5 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Escherichia coli (strain K12) (see paper)
29% identity, 81% coverage: 7:133/157 of query aligns to 298:423/443 of P0A6C5

query
sites
P0A6C5

V

I

R

R

K

R

A

A

R

T

I

I

S

N

D

D

V

I

K

G

A

G

M

I

H

-

A

-

M

L

L

E

L

L

D

I

N

R

P

P

-

L

E

E

E

Q

E

Q

G

G

L

I

V

L

L

V

P

R

R

R

S

S

F

R

N

E

K

Q

L

L

Y

E

T

M

L

E

L

I

R

-

D

D

F

K

F

F

V

T

V

I

V

I

E

Q

K

R

N

D

S

N

G

T

E

T

L

I

A

A

G

-

C

C

C

A

A

A

L

L

S

Y

I

P

C

F

W

P

E

E

E

E

-

K

L

I

A

G

E

E

V

M

R

A

S

C

L

V

V

A

V

V

R

H

N

P

T

D

F

Y

R

R

G

S

R

S

G

S

L

R

G

G

R

E

R

V

L

L

V

L

D

E

A

R

C

I

L

A

S

A

E

Q

A

A

V

K

T

Q

L

S

E

G

L

L

Y

S

K

K

V

L

F

F

V

V

L

L

T

T

N

T

-

R

T

S

P

I

E

H

F

W

F

F

A

Q

H

E

L

R

G

G

F

F

R

T

E

P

V

V

P

D

K

I

D

D

V

L

L

L

P

P

Q

E

Sites not aligning to the query:

15 H→Y: In EE17.
19 Y→C: In EE51.
54 S→N: In PT2M217.
58 R→H: In EE11.
287 G→S: In PT2M216.
432 Q→R: In PT2M217.

Query Sequence

>WP_144019464.1 NCBI__GCF_900167125.1:WP_144019464.1
MDHDFVVRKARISDVKAMHAMLLDNPEEEGLVLPRSFNKLYTLLRDFFVVVEKNSGELAG
CCALSICWEELAEVRSLVVRNTFRGRGLGRRLVDACLSEAVTLELYKVFVLTNTPEFFAH
LGFREVPKDVLPQKVWSDCLNCPKFPDCDEIAMMLRL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory