SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_147050548.1 NCBI__GCF_001482365.1:WP_147050548.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
31% identity, 99% coverage: 3:317/317 of query aligns to 7:279/285 of 4yerA

query
sites
4yerA

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binding adenosine-5'-diphosphate: F14 (= F10), F17 (≠ V25), N39 (= N47), G40 (= G48), G42 (= G50), K43 (= K51), T44 (= T52), T45 (= T53), T135 (≠ R138), F136 (≠ L139), S137 (= S140)

P34358 ABC transporter ced-7; Cell death protein 7 from Caenorhabditis elegans (see 2 papers)
38% identity, 62% coverage: 26:221/317 of query aligns to 561:760/1704 of P34358

query
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P34358

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K586 (= K51) mutation to R: Cell corpses not engulfed.
E639 (= E104) mutation to G: Cell corpses not engulfed.

Sites not aligning to the query:

126 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1012 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1045 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1417 K→R: Some cell corpses not engulfed.

7roqA Alternative structure of human abca1
37% identity, 61% coverage: 28:221/317 of query aligns to 787:984/1831 of 7roqA

query
sites
7roqA

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Sites not aligning to the query:

binding cholesterol: 688, 1106, 1426, 1526, 1527

8k1oB Mycobacterial efflux pump, amppnp bound state
36% identity, 70% coverage: 1:222/317 of query aligns to 6:215/215 of 8k1oB

query
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8k1oB

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binding phosphoaminophosphonic acid-adenylate ester: N40 (= N47), G43 (= G50), K44 (= K51), S45 (≠ T52), T46 (= T53), T131 (≠ R138), S133 (= S140)

8k1pB Mycobacterial efflux pump, adp+vanadate bound state
36% identity, 70% coverage: 1:222/317 of query aligns to 4:213/213 of 8k1pB

query
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8k1pB

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binding adenosine-5'-diphosphate: F13 (= F10), N38 (= N47), G39 (= G48), A40 (= A49), G41 (= G50), K42 (= K51), S43 (≠ T52), T44 (= T53)
binding adenosine-5'-triphosphate: T129 (≠ R138), S131 (= S140)

8ee6A Cryo-em structure of human abca7 in pe/ch nanodiscs (see paper)
38% identity, 70% coverage: 1:221/317 of query aligns to 624:838/1808 of 8ee6A

query
sites
8ee6A

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D

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A

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G

T

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A

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A

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A

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R

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C

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Y

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G

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-

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-

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A

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A

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-

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S

binding phosphothiophosphoric acid-adenylate ester: F633 (= F10), A640 (= A27), N660 (= N47), G661 (= G48), G663 (= G50), K664 (= K51), T665 (= T52), T666 (= T53)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 607, 608, 1507, 1514, 1535, 1536, 1537, 1538, 1539

O95477 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein; EC 7.6.2.1 from Homo sapiens (Human) (see 35 papers)
37% identity, 62% coverage: 27:221/317 of query aligns to 915:1114/2261 of O95477

query
sites
O95477

A

A

V

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D

G

G

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N

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N

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K

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x
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x
M

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S

D917 (= D29) to Y: in a colorectal cancer sample; somatic mutation
T929 (= T41) to I: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane; dbSNP:rs1832457117
N935 (= N47) to S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs28937313
K939 (= K51) mutation to M: Inhibits ATPase activity; when associated with M-1952. Decreases translocase activity; when associated with M-1952. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-1952.
S1042 (≠ A148) modified: Phosphoserine; by PKA
P1065 (= P171) natural variant: P -> S
M1091 (≠ L198) to T: in FHA1; loss of localization to plasma membrane; decreased cholesterol efflux; decreased phospholipid efflux
C1110 (≠ M217) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1111.
C1111 (≠ A218) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1110.

Sites not aligning to the query:

3 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased cholesterol efflux. Decreased phospholipid efflux. Decreased palmitoylation; when associated with S-23, S-1110 and S-1111.
23 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased palmitoylation; when associated with S-3, S-1110 and S-1111.
74 I→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.; I→K: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-371.
75 modified: Disulfide link with 309
85 P → L: in FHA1; Alabama; dbSNP:rs145183203
98 modified: carbohydrate, N-linked (GlcNAc...) asparagine
100 S→C: Highly decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
210 E → D: in a colorectal cancer sample; somatic mutation
219 R → K: in dbSNP:rs2230806
230 R → C: in dbSNP:rs9282541
244 modified: carbohydrate, N-linked (GlcNAc...) asparagine
248 P → A: in dbSNP:rs142625938
255 A → T: in TGD; deficient cellular cholesterol efflux; dbSNP:rs758100110
304 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-308.
308 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-304.
309 modified: Disulfide link with 75
364 S → C: in dbSNP:rs775035559
371 I→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane. 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-74 or C-375.; I→E: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-74.
375 L→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.
399 V → A: in dbSNP:rs9282543
401 K → Q: in dbSNP:rs138487227
496 R → W: in dbSNP:rs147675550
568 K→A: 60-65% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane.
573 Y→F: No effect on phospholipid and cholesterol efflux and on localization to cell membrane.
581 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-584 and K-585.
583 F→K: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-590.
584 E→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-585.
585 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-584.
590 W → S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs137854496; W→E: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-583.
593 F→L: Moderately decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
638 R → Q: in dbSNP:rs374190304
771 V → M: in dbSNP:rs2066718
774 T → P: in dbSNP:rs35819696; natural variant: T -> S
776 K → N: in dbSNP:rs138880920
815 E → G: in dbSNP:rs145582736
825 V → I: in dbSNP:rs2066715
883 I → M: in dbSNP:rs2066714
1172 E → D: in dbSNP:rs33918808
1181 S → F: in dbSNP:rs76881554
1216 G → V: in dbSNP:rs562403512
1341 R → T: in dbSNP:rs147743782
1376 S → G: in dbSNP:rs145689805
1379 L → F: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane; dbSNP:rs1831213945
1407 A → T: in a colorectal cancer sample; somatic mutation; dbSNP:rs189206655
1463 modified: Disulfide link with 1477
1477 modified: Disulfide link with 1463; C → R: in TGD; loss of interaction with APOE; unable to generate APOE-containing high density lipoproteins; moderately decreased protein abundance; moderately decreased ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs137854494
1512 T→M: Moderately decreased protein abundance. Does not affect ATPase activity. Moderately decreased phospholipid translocase activity.
1555 I → T: in dbSNP:rs1997618
1587 K → R: in dbSNP:rs2230808
1611 N → D: in FHA1; deficient cellular cholesterol efflux
1615 R → Q: in dbSNP:rs1251839800
1648 L → P: in dbSNP:rs1883024
1670 A → T: in dbSNP:rs1203589782
1680 R → Q: in dbSNP:rs150125857
1704 V → D: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
1731 S → C: in dbSNP:rs760507032
1897 R → W: in FHA1; uncertain significance; dbSNP:rs760768125
1925 R → Q: in Scott syndrome; shows impaired trafficking of the mutant protein to the plasma membrane; dbSNP:rs142688906
1952 K→M: Inhibits ATPase activity; when associated with M-939. Decreases translocase activity; when associated with M-939. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-939.
2054 modified: Phosphoserine; by PKA
2081 R → W: in TGD; highly decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane; dbSNP:rs137854501
2109 A → T: in a colorectal cancer sample; somatic mutation
2150 P → L: in FHA1; moderately decreased protein abundance; does not affect ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs369098049
2163 natural variant: F -> S
2168 L → P: in dbSNP:rs2853577
2243 D → E: in dbSNP:rs34879708
2244 V → I: in dbSNP:rs144588452

P41233 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; EC 7.6.2.1 from Mus musculus (Mouse) (see paper)
37% identity, 62% coverage: 27:221/317 of query aligns to 915:1114/2261 of P41233

query
sites
P41233

A

A

V

V

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D

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G

L

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A

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N

A

F

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N

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A

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S

Sites not aligning to the query:

489 modified: carbohydrate, N-linked (GlcNAc...) asparagine

8eopA Cryo-em structure of nanodisc reconstituted human abca7 eq mutant in atp bound closed state (see paper)
38% identity, 70% coverage: 1:221/317 of query aligns to 650:864/1687 of 8eopA

query
sites
8eopA

M

V

S

S

I

V

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R

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S

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L

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K

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F
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F

P

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A

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R

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-

G

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A

-

G

G

D
x
S

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A
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A

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N

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G

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K

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T

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x
H

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S

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M

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S

binding adenosine-5'-triphosphate: F659 (= F10), S662 (≠ D23), A666 (= A27), N686 (= N47), G687 (= G48), G689 (= G50), K690 (= K51), T691 (= T52), T692 (= T53), H782 (≠ R138), S784 (= S140)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1461, 1488, 1489, 1491, 1492, 1494, 1533, 1584, 1586
binding magnesium ion: 1493, 1533

7tbwA The structure of atp-bound abca1 (see paper)
36% identity, 62% coverage: 27:221/317 of query aligns to 770:969/1928 of 7tbwA

query
sites
7tbwA

A

A

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V

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D

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G

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E

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F

L

L

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G

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N

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G

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A

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K

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T

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R

D

L

V

G

G

I

L

A

P

A

S

F

S

D

K

-

L

R

K

T

S

V

K

V

T

R

S

R
x
Q

L

L

S
|
S

G

G

Q

M

R

Q

Q

R

R

K

L

L

A

S

L

V

A

A

A

L

A

A

L

F

A

V

G

G

D

G

P

S

E

K

V

V

F

I

L

L

D
|
D

E
x
Q

P

P

S

T

A

A

G

G

L

V

D

D

P

P

Q

Y

S

S

R

R

H

R

I

G

V

I

F

W

D

E

L

L

I

L

G

L

Q

K

L

Y

R

-

R

R

E

Q

G

G

R

R

C

T

I

I

L

L

T

S

T

T

H
|
H

L

H

L

M

D

D

D

E

A

A

Q

D

R

V

L

L

A

G

D

D

R

R

V

I

C

A

I

I

V

I

D

S

H

H

G

G

R

K

V

L

M

C

A

C

E

V

G

G

S

S

binding adenosine-5'-triphosphate: N790 (= N47), G791 (= G48), G793 (= G50), K794 (= K51), T795 (= T52), Q835 (= Q92), Q887 (≠ R138), S889 (= S140), H944 (= H196)
binding magnesium ion: Q835 (= Q92), D912 (= D163), Q913 (≠ E164)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 763, 765, 768, 1630, 1633, 1657, 1658, 1660, 1661, 1662, 1663, 1702, 1753, 1755, 1756, 1757, 1779, 1783, 1811
binding cholesterol: 218, 219, 360, 367
binding magnesium ion: 1662, 1702

8f5bA Human abca4 structure in complex with amp-pnp (see paper)
37% identity, 62% coverage: 27:221/317 of query aligns to 737:935/1924 of 8f5bA

query
sites
8f5bA

A

A

V

V

D

D

G

R

L

L

S

N

L

I

T

T

A

F

R

Y

H

E

G

N

E

Q

V

I

T

T

A

A

L

F

L

L

G

G

P

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

T
|
T

T

T

L

L

E

S

C

I

A

L

Q

T

G

G

L

L

Q

L

R

P

P

P

D

T

A

S

G

G

T

T

V

V

R

L

L

V

L

G

G

G

Q

R

D

D

P

I

W

E

R

T

A

S

S

L

P

D

A

A

L

V

R

R

A

Q

R

S

V

L

G

G

V

M

M

C

L

P

Q
|
Q

D

H

G

N

G

I

L

L

P

F

Q

H

A

H

V

L

R

T

P

V

L

A

E

E

L

H

L

M

R

L

H

F

V

Y

C

A

A

Q

L

L

-

K

-

G

-

K

-

S

-

Q

H

E

R

E

S

A

P

Q

R

L

D

E

M

M

D

E

E

A

L

M

V

L

E

E

R

D

L

T

G

G

I

L

A

H

F

K

D

R

R

N

T

E

V

E

V

A

R

Q

R
x
D

L

L

S

S

G

G

Q

M

R

Q

Q

R

R

K

L

L

A

S

L

V

A

A

A

I

A

A

L

F

A

V

G

G

D

D

P

A

E

K

V

V

F

I

L

L

D

D

E

E

P

P

S

T

A

S

G

G

L

V

D

D

P

P

Q

Y

S

S

R

R

H

R

I

S

V

I

F

W

D

D

L

L

I

L

G

L

Q

K

L

Y

R

-

R

R

E

S

G

G

R

R

C

T

I

I

L

M

T

S

T

T

H

H

L

H

L

M

D

D

D

E

A

A

Q

D

R

L

L

L

A

G

D

D

R

R

V

I

C

A

I

I

V

I

D

A

H

Q

G

G

R

R

V

L

M

Y

A

C

E

S

G

G

S

T

binding phosphoaminophosphonic acid-adenylate ester: N757 (= N47), G758 (= G48), G760 (= G50), K761 (= K51), T762 (= T52), T763 (= T53), Q802 (= Q92), D853 (≠ R138)
binding magnesium ion: T762 (= T52), Q802 (= Q92)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 1652, 1653, 1654, 1655, 1656, 1657, 1697, 1748, 1750
binding magnesium ion: 1657, 1697

7e7oA Cryo-em structure of human abca4 in nrpe-bound state (see paper)
37% identity, 62% coverage: 27:221/317 of query aligns to 824:1022/2003 of 7e7oA

query
sites
7e7oA

A

A

V

V

D

D

G

R

L

L

S

N

L

I

T

T

A

F

R

Y

H

E

G

N

E

Q

V

I

T

T

A

A

L

F

L

L

G

G

P

H

N

N

G

G

A

A

G

G

K

K

T

T

L

L

E

S

C

I

A

L

Q

T

G

G

L

L

Q

L

R

P

P

P

D

T

A

S

G

G

T

T

V

V

R

L

L

V

L

G

G

G

Q

R

D

D

P

I

W

E

R

T

A

S

S

L

P

D

A

A

L

V

R

R

A

Q

R

S

V

L

G

G

V

M

M

C

L

P

Q

Q

D

H

G

N

G

I

L

L

P

F

Q

H

A

H

V

L

R

T

P

V

L

A

E

E

L

H

L

M

R

L

H

F

V

Y

C

A

A

Q

L

L

-

K

-

G

-

K

-

S

-

Q

H

E

R

E

S

A

P

Q

R

L

D

E

M

M

D

E

E

A

L

M

V

L

E

E

R

D

L

T

G

G

I

L

A

H

F

K

D

R

R

N

T

E

V

E

V

A

R

Q

R

D

L

L

S

S

G

G

Q

M

R

Q

Q

R

R

K

L

L

A

S

L

V

A

A

A

I

A

A

L

F

A

V

G

G

D

D

P

A

E

K

V

V

F

I

L

L

D

D

E

E

P

P

S

T

A

S

G

G

L

V

D

D

P

P

Q

Y

S

S

R

R

H

R

I

S

V

I

F

W

D

D

L

L

I

L

G

L

Q

K

L

Y

R

-

R

R

E

S

G

G

R

R

C

T

I

I

L

M

T

S

T

T

H

H

L

H

L

M

D

D

D

E

A

A

Q

D

R

L

L

L

A

G

D

D

R

R

V

I

C

A

I

I

V

I

D

A

H

Q

G

G

R

R

V

L

M

Y

A

C

E

S

G

G

S

T

Sites not aligning to the query:

binding [(2S)-3-[2-[(E)-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenylidene]amino]ethoxy-oxidanyl-phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxy-propyl] (Z)-octadec-9-enoate: 285, 290, 526, 527, 589, 592, 1454

F1MWM0 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM protein; RmP; Retinal-specific ATP-binding cassette transporter; EC 7.6.2.1 from Bos taurus (Bovine) (see 2 papers)
37% identity, 62% coverage: 27:221/317 of query aligns to 945:1143/2281 of F1MWM0

query
sites
F1MWM0

A

A

V

V

D

D

G

R

L

L

S

N

L

I

T

T

A

F

R

Y

H

E

G

S

E

Q

V

I

T

T

A

A

L

F

L

L

G

G

P

H

N

N

G

G

A

A

G

G

K

K

T

T

L

L

E

S

C

I

A

M

Q

T

G

G

L

L

Q

L

R

P

P

P

D

T

A

S

G

G

T

T

V

V

R

L

L

V

L

G

G

G

Q

K

D

D

P

I

W

E

R

T

A

N

S

L

P

D

A

A

L

I

R

R

A

Q

R

S

V

L

G

G

V

M

M

C

L

P

Q

Q

D

H

G

N

G

I

L

L

P

F

Q

H

A

H

V

L

R

T

P

V

L

A

E

E

L

H

L

I

R

L

H

F

V

Y

C

A

A

Q

L

L

H

K

-

G

-

R

-

S

-

W

-

D

R

K

S

A

P

Q

R

L

D

E

M

M

D

E

E

A

L

M

V

L

E

E

R

D

L

T

G

G

I

L

A

H

F

K

D

R

R

N

T

E

V

E

V

A

R

Q

R

D

L

L

S

S

G

G

Q

V

R

Q

Q

R

R

K

L

L

A

S

L

V

A

A

A

I

A

A

L

F

A

V

G

G

D

D

P

A

E

K

V

V

F

V

L

L

D

D

E

E

P

P

S

T

A

S

G

G

L

V

D

D

P

P

Q

Y

S

S

R

R

H

R

I

S

V

I

F

W

D

D

L

L

I

L

G

L

Q

K

L

Y

R

-

R

R

E

S

G

G

R

R

C

T

I

I

L

M

T

S

T

T

H

H

L

H

L

M

D

D

D

E

A

A

Q

D

R

I

L

L

A

G

D

D

R

R

V

I

C

A

I

I

V

I

D

S

H

Q

G

G

R

R

V

L

M

Y

A

C

E

S

G

G

S

T

Sites not aligning to the query:

415 modified: carbohydrate, N-linked (Hex...) asparagine
504 modified: carbohydrate, N-linked (Hex...) asparagine
901 modified: Phosphothreonine; T→A: Decreases expression level. Affects subcellular location.
1185 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1309 Cleavage; by trypsin
1313 modified: Phosphothreonine; T→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1317 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Affects both the basal and stimulated ATPase activity.
1319 modified: Phosphoserine
1455 modified: carbohydrate, N-linked (Hex...) asparagine
1527 modified: carbohydrate, N-linked (Hex...) asparagine
1660 modified: carbohydrate, N-linked (Hex...) asparagine

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 43 papers)
37% identity, 62% coverage: 27:221/317 of query aligns to 945:1143/2273 of P78363

query
sites
P78363

A
|
A

V
|
V

D
|
D

G
x
R

L
|
L

S
x
N

L
x
I

T
|
T

A
x
F

R
x
Y

H
x
E

G
x
N

E
x
Q

V
x
I

T
|
T

A
|
A

L
x
F

L
|
L

G
|
G

P
x
H

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

T
|
T

L
|
L

E
x
S

C
x
I

A
x
L

Q
x
T

G
|
G

L
|
L

Q
x
L

R
x
P

P
|
P

D
x
T

A
x
S

G
|
G

T
|
T

V
|
V

R
x
L

L
x
V

L
x
G

G
|
G

Q
x
R

D
|
D

P
x
I

W
x
E

R
x
T

A
x
S

S
x
L

P
x
D

A
|
A

L
x
V

R
|
R

A
x
Q

R
x
S

V
x
L

G
|
G

V
x
M

M
x
C

L
x
P

Q
|
Q

D
x
H

G
x
N